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54 results on '"Changku Jia"'

51. [The effect of an antioxidant tea polyphenols on cell apoptosis in rat model of cyclosporine-induced chronic nephrotoxicity]

Author

Shaohua, Shi, Shusen, Zheng, Changku, Jia, Youfa, Zhu, and Haiyang, Xie

Subjects
Flavonoids, Male, Tea, Caspase 3, Polymers, Polyphenols, Apoptosis, Kidney, Antioxidants, Rats, Rats, Sprague-Dawley, Phenols, Caspases, Cyclosporine, In Situ Nick-End Labeling, Animals, RNA, Messenger, Immunosuppressive Agents
Abstract

To investigate the effect of tea polyphenols on cell apoptosis in rat model of cyclosporine-induced chronic nephrotoxicity.Four groups of animals in rat model of cyclosporine-induced chronic nephrotoxicity were respectively treated by olive oil (n = 6), tea polyphenols (TP, n = 6), cyclosporine A (CsA, n = 8) and TP plus CsA (n = 8). At the end of 28th day of treatment, all animals were sacrificed and blood was analyzed for blood serum creatinine and creatinine clearance, kidney tissue for pathologic analysis. The TUNEL assay, caspase-3 mRNA expression detected by reverse transcription-polymerase chain reaction (RT-PCR) and caspase-3 activity were used for the analysis of cell apoptosis.CsA plus TP ameliorated the CsA-induced decrease of renal function and interstitial fibrosis. There was a significant increase in the number of apoptosis-positive cells in the CsA-vs-CsA plus TP-treated group at four weeks (18.9 +/- 3.3 vs. 7.7 +/- 1.4, P0.05). The expression of caspase-3 mRNA and caspase-3 activity of CsA-treated group was significantly higher than that of CsA plus TP-treated group (P0.05).These results indicate that antioxidant tea polyphenols significantly inhibit apoptosis of tubular and interstitial cells in rat model of chronic cyclosporine-induced nephrotoxicity, and suggest that the decrease of cell apoptosis exerted by tea polyphenols may be one of mechanisms to protect renal function and tissue structure.

Published
2002

53. Effect of 1,25-dihydroxyvitamin D3 on preventing allograft from acute rejection following rat orthotoic liver transplantation

Author

Shusen Zheng, Changku Jia, Yan Wang, and Ai-Bin Zhang

Subjects
Graft Rejection, Male, Calcitriol, medicine.medical_treatment, Gene Expression, chemistry.chemical_element, Liver transplantation, Pharmacology, Calcium, Rats sprague dawley, Rats, Sprague-Dawley, Interferon-gamma, Th2 Cells, Adjuvants, Immunologic, Gene expression, polycyclic compounds, Animals, Transplantation, Homologous, Medicine, Interferon gamma, RNA, Messenger, Rats, Wistar, Graft rejection, business.industry, Gastroenterology, General Medicine, Interleukin-10, Liver Transplantation, Rats, Interleukin 10, Basic Research, surgical procedures, operative, chemistry, Acute Disease, Immunology, lipids (amino acids, peptides, and proteins), business, medicine.drug
Abstract

To study the mechanism and the preventive role of 1,25-dihydroxyvitamin D3 in acute rejection following orthotopic liver transplantation.Rats were randomly divided as donors or recipients for orthotopic liver allotransplantation model. Four groups were designed in the study, Group I: syngenic control (Wistar to Wistar); Group II: acute rejection (SD to Wistar); Group III: acute rejection treated with cyclosporine A, and Group IV: acute rejection treated with 1,25-(OH)2D3. Liver function, rejection activity index and mRNA of IFN-gamma, IL-10 in intragraft in recipients were measured in on day 1, 5, 7, 15, 30 posttransplant for assessing graft function, severity of acute rejection and immune state of recipients.Survival time of recipients in Group VI was significantly prolonged (over 100 days) in comparison with other groups (vs Group II, P0.001; vs Group III, P0.05). After treatment with 1,25-(OH)2 D3, mean value of all the assay tested on each experimental time was compared, liver function in group IV was significantly improved (AST 127+/-41 U/L-360+/-104 U/L, BIL 13+/-5 mmol/l-38+/-11 mmol/l; vs Group II, P0.05; vs Group III, P0.05. Rejection activity index was significantly decreased (0-3.3+/-1.6; vs Group II, P0.05; vs Group III, P0.05). Level of hepatic IFN-gamma mRNA in group IV was decreased, while level of hepatic IL-10 mRNA was increased (vs Group II, P0.05; vs Group III, P0.05).Our results indicated that 1,25-(OH)2D3 induced the secretion of cytokine toward to Th2 type, which would alleviate acute rejection, protect liver function and prolong survival of recipient after orthotopic liver transplantation.

Published
2003

54. Upper gastric tract bleeding due to cholecystoduodenal fistula: a case report.

Author

Changku J, Shaohua S, Zhicheng Z, and Shusen Z

Subjects
Chronic Disease, Gastroscopy, Hemostasis, Surgical, Humans, Intestinal Fistula diagnosis, Male, Melena etiology, Middle Aged, Cholecystitis complications, Gastrointestinal Hemorrhage etiology, Intestinal Fistula complications
Abstract

A 50-year-old man was admitted to our hospital because of a single episode of hematemesis followed by melena for about 14 hours. On gastroendoscopy a 1.2 x 1.4 cm2 deep ulcer was visualized in the back wall of the first part of the duodenum with active bleeding. Digital subtracted angiography showed an obvious leakage of contrast agents from the gallbladder artery. So hemobilia and cholecystoduodenal fistula with upper gastric tract bleeding were diagnosed. Cholecystectomy was performed after the duodenal fistula was resected and repaired. Common bile duct was intubated with a T-tube after no bleeding and stones were verified inside. A pathologic examination confirmed the presence of chronic cholecystitis with the formation of suppurate granulomatosis. The postoperative course was uneventful. He has been doing well without any recurrence of bleeding for the last 7 months.

Published
2005

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