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In recent years, residue-to-energy utilization has become an important energy source that can alleviate the reliability of fossil fuel, and agricultural residue (AR) transportation has great potential for optimization in the AR supply chain. However, various methods presented make their correct implementation difficult for sustainable optimization. Therefore, four key issues of the AR transportation for bioenergy production have been thoroughly reviewed: (i) distribution of AR in cropland, (ii) estimation of transportation distance, (iii) variability of vehicle arrangements, and (iv) (de)centralized transportation patterns. This review has several findings. To begin with, land cover classification method with remote sensing technology is suggested to locate the distribution of AR, thereby being helpful for facilitates decision-making optimization. Second, electronic navigator applications (Google Maps or Baidu Maps) are recommended for transportation distance estimation, and the implementation of manipulating Baidu Maps with R is provided. Third, the vehicle selection bias would bring estimation gaps, so a vehicle database is required to satisfy the local needs. And finally, transportation distance, the requirement of AR pretreatments and brokers’ participation can affect the decision of (de)centralized transportation patterns. The findings and technical details discussed are especially useful for researchers and bioenergy project investors who intend to reduce the AR transportation cost in the future, especially for developing counties.

Objectives: Intracranial atherosclerotic stenosis (ICAS) is one of the most common causes of stroke worldwide and, in particular, has been implicated as a leading cause of recurrent ischemic stroke. We adapted a rat model of atherosclerosis to study brain intracranial atherosclerosis, and further investigated the effect of omega-3 fatty acids (O3FA) in attenuating development of ICAS. Materials and methods: Adult male Sprague–Dawley rats were divided into control normal-cholesterol or high-cholesterol diet groups with or without O3FA for up to 6 weeks. During the first 2 weeks, NG-nitro- l -arginine methyl ester ( l -NAME, 3 mg/mL) was added to the drinking water of the high-cholesterol groups. The rats received supplementation with O3FA (5 mg/kg/day) by gavages. Blood lipids including low density lipoprotein (LDL), cholesterol (CHO), triglycerides (TG) and high density lipoprotein (HDL) were measured at 3 and 6 weeks. The lumen of middle cerebral artery (MCA) and the thickness of the vessel wall were assessed. Inflammatory molecular markers were assessed by Western blot. Results: A high-cholesterol diet exhibited a significant increase in the classic blood markers (LDL, CHO, and TG) for atherosclerosis, as well as a decrease in HDL. These markers were found to be progressively more severe with time. Lumen stenosis and intimal thickening were increased in MCA. O3FA showed attenuation of blood lipids with an absence of morphological changes. O3FA significantly reduced the inflammatory marker CD68 in MCA and prevented monocyte chemotactic protein (MCP-1) and interferon-γ (IFN-γ) expression in the brain. O3FA similarly decreased inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), and interleukin 6 (IL-6), markers affiliated with monocyte activity in atherosclerosis. Furthermore, O3FA significantly inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1), a marker for endothelial activation. Lastly, O3FA increased ATP-binding cassette transporter A1 (ABCA1) protein expression via silent information regulator 1 (SIRT1) activation, thus increasing cholesterol efflux from macrophages to HDL. Conclusions: Long-term O3FA dietary supplementation prevents the development of intracranial atherosclerosis. This O3FA effect appears to be mediated by its prevention of macrophage infiltration into the vessel wall, therefore reducing inflammation and intimal thickening. While similar effects in humans need to be determined, O3FA dietary supplement shows promising results in the prevention of ICAS.

Intracranial atherosclerotic stenosis (ICAS) is one of the most common causes of stroke worldwide and, in particular, has been implicated as a leading cause of recurrent ischemic stroke. We developed a new rat model to study intracranial atherosclerosis.Twelve-week-old male Sprague-Dawley rats were divided into a control (on a maintain diet) and a high-cholesterol group (on a daily 1% cholesterol diet) for up to 6 weeks. During the first two weeks, NG-nitro-L-arginine methylester (L-NAME, 3 mg/mL) was added to the drinking water in the high-cholesterol group to induce intimal changes making the rats susceptible to atherosclerosis. Blood lipids, including low-density lipoprotein (LDL), cholesterol (CHO), triglycerides (TG), and high-density lipoprotein (HDL), were measured after 3 and 6 weeks. Histological sections of the brains, including internal carotid artery (ICA), middle cerebral artery (MCA), and basilar artery (BA), were prepared to study intracranial artery morphometry and intimal thickening. The levels of CD68, an inflammatory marker, within the vessel walls as determined by immunohistochemistry were also measured.The high-cholesterol diet increased the levels of classic blood markers of atherosclerosis, LDL, CHO, and TG as well as decreased HDL, which became progressively more intensive with time. Rats showed increased intimal thickening in the ICA, MCA, and BA. This protocol also increased the levels of CD68 immunoreactivity within the vessel walls.A rat model of intracranial atherosclerosis was effectively developed by high-cholesterol diet and L-NAME administration. This clinically relevant model would be beneficial for studying ICAS.

A preliminary conceptual framework is proposed for the estimation of the costs of desertification. This framework consists of assessment of the extent and severity of desertification, identification of various impacts and valuation of the direct and indirect costs of desertification. Based on this framework, three well-cited studies of the costs of desertification in China were critically reviewed. Limitations in these studies include double counting, confusion of short-term and long-term costs and the lack of necessary adjustment of price parameters. Refinement of the estimates indicates that these limitations have caused serious overestimation or underestimation of the costs of desertification. To achieve more reliable and useful estimates, future studies need to overcome the limitations mentioned in the preceding texts and use the latest available data on desertification and its various impacts. A more comprehensive conceptual framework that includes the valuation of dryland ecosystem services could also be developed to guide future studies. Copyright © 2016 John Wiley & Sons, Ltd.

The Green Manure Planting Program (GMP), which is a typical case of payment for farmland ecosystem services, has come into play on conserving paddy fields in China. This paper analyses farmers’ willingness to accept (WTA) for adopting GMP by employing the contingent valuation method (CVM) and Double-hurdle (D-H) model on the basis of a sample data set containing information on 1217 farmers in five provinces in southern paddy fields region (SPF) of China. The results show that farmers’ WTA for adopting GMP was 3323 CNY/ha per year, which is much higher than the current compensation standards (com-standards) in the study region. This result explains why the current policy does invalidly stimulate farmers for adopting GMP. Farmers’ WTA is not only influenced by the cost-benefit effect of GMP adoption, but also impacted by their perceived environmental value in improving the quality of paddy fields as well as the demographic and socioeconomic information such as farmers’ age, political identities, income and paddy field area. It is costly if the government compensates all farmers who adopt GMP, letting farmers aware of more environmental value of GMP and maximally promoting the transformation of these environmental values into economic benefits is an effective measure to encourage them to adopt GMP.The results of this study can guide policy makers in determining the budget for GMP compensation and designing systematic compensation mechanism hence improve the sustainability of paddy fields conservation.

In the highest soil erosion regions of arid and semiarid northwest China, water resource deficits and farmland misuse have further exacerbated soil degradation. Therefore, understanding how farmers in diverse agroclimatic zones perceive and respond to different conservation practices is important to the implementation of sustainable agriculture practices (SAPs). To this end, this study uses a best&ndash, worst scaling approach to examine the adoption preferences for nine SAPs among grain and cash crop farmers and investigates the influence of farm and climatic characteristics on adoption preferences based on a face to face survey of 554 households in Gansu province, which is classified as an arid and semiarid area in northwest China. Both grain and cash crop farmers had stronger preferences for the practices of using organic instead of chemical fertilizers and of improving irrigation practices. In addition, while cash crop farmers also had strong preferences for cover crop-related practices, they preferred long-term fallows least. Household income, livestock, and precipitation influence the potential perceived importance of SAPs. The different perceived importance of these practices suggests new possible combinations or packages for a sustainable agriculture program during the cropping structure adjustment in Gansu.

The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size (e.g., length and width) when breeding novel rice varieties. It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines (RILs) and reciprocal single chromosomal segment substitution lines (SSSLs) derived from the progeny of a japonica Asominori × indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured, namely length and flag leaf, length and flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for marker-assisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice.

Urban households account for most of the milk consumption in China, but their consumption is hampered by safety concerns. Using survey data collected in Beijing and Harbin in 2010, this paper simultaneously analyses urban households' milk consumption using a multiple linear model and their willingness-to-pay for milk safety using an ordered choice model. The results of this study show that as income increases, urban households consume more milk and are willing to pay a higher premium for milk safety. Modern food marketing channels play a positive role in stimulating milk consumption and building consumers' confidence in milk safety. The growth in the elderly population influences milk consumption positively, but their demand for milk safety is negatively affected by higher price. The combined analysis of households' demand for milk quantity and safety may be useful to the Chinese government in promoting the development of the domestic milk industry and to dairy firms in exploring the milk market in China.

Planting green manure in fallow croplands in winter can bring various economic and environmental benefits, including increased food production, carbon capture and sequestration, soil retention, sandstorm prevention, water retention, and provision of habitat for biodiversity. However, the increased production cost of planting green manure reduces farmers&rsquo, willingness to adopt this approach, which is unfavorable for its sustainability. This research aims to investigate the influence of instrumental variables on farmers&rsquo, perceptions of sustainable agriculture practices, especially the use of rotation fallow, and tries to understand the relationship between farmers&rsquo, perceptions of using rotation fallow and planting green manure under incentive measures adopted by local authorities in Guangxi Province, China. Using simultaneous equation models, the results show that subsidies and planting training were the most important drivers for restoring green manure planting in the target region. These incentive measures could be further enhanced as a priority to restore green manure planting. The study also finds that socioeconomic factors such as farmer&rsquo, s income, area of farmland, and labor for agricultural production have a certain influence on planting green manure planting and on farmers&rsquo, perceptions of using rotation fallow as a form of sustainable agriculture practice.

Exploitation of heterosis has brought significant advance in plant breeding and agricultural production, although its genetic basis is still poorly understood. In this study, a total of 66 chromosome segment substitution (CSS) lines, derived from a cross between japonica rice inbred line Asominori (as the recurrent parent) and indica rice inbred line IR24 (as the donor parent), were used to investigate the genetic basis of heterosis in indica × japonica inter-subspecific rice hybrids. Each CSS line was crossed with the background parent Asominori, and the heterosis of F(1) hybrids was estimated by comparing the F(1) performance with its two parental lines. Field experiments were carried out across six different environments to evaluate yield and yield-related traits in the 66 CSS lines and their 66 corresponding F(1) hybrids. Quantitative trait loci (QTL) analyses were conducted using a likelihood ratio test based on the stepwise regression. Thirty-six QTL were identified with significant effects in CSSL, 21 with significant effects in hybrids and 13 with significant effects in both. On the basis of average dominance degree, of all the 70 QTL affecting yield-related agronomic traits, 28.6% (20) showed an overdominance, 35.7% (25) a partial dominance and 30% (21) an additive effect, indicating that all effects contribute to trait variation in japonica-indica rice hybrids. Effects of these QTL were examined to identify Indica rice chromosome segments of interest for the improvement of japonica inbred lines and hybrids.

Key message This article used seven characters from the 2D image analysis to dissect the genetic architecture underlying rice grain shape in onejaponica × indicapopulation consisting of 215 recombinant inbred lines. Abstract Two-dimensional (2D) digital image analysis is efficient for investigating the rice grain shape characters in large genetic and breeding populations. In this study, we used 2D image analysis to investigate seven characters, i.e., grain length (GL), grain width (GW), length-to-width ratio (LW), grain area (GA), grain circumference (GC), grain diameter (GD), and grain roundness (GR), in one japonica × indica genetic population consisting of 215 recombinant inbred lines. GL and GW can be recorded manually as well, and have been extensively used together with LW (i.e., GL/GW) in genetic studies on grain shape. GC and GA can be hardly measured manually, and have not been used together with GD and GR. Results indicated that the seven characters could be precisely measured by 2D image analysis, genotype by environment interaction was low, and heritability was high. Each character was controlled by a few major stable genes and multiple minor additive genes. A total of 51 QTL were detected for the seven characters across four diverse environments, 22 from GL, GW, and LW, the three traditional characters, and 29 from the other four characters. The 51 QTL were clustered in eighteen marker intervals. Comparing with previous studies and analyzing the stability of identified QTL, we found six non-reported marker intervals, one each on chromosomes 2 and 3, and two each on chromosomes 6 and 8. The newly identified loci and the large-scale phenotyping system would greatly improve our knowledge about the genetic architecture and the future rice breeding on grain shape. Electronic supplementary material The online version of this article (doi:10.1007/s00122-015-2560-7) contains supplementary material, which is available to authorized users.