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PurposeThe main aim of this study is to explore the moderating role of ethical leadership on the indirect effect of work–family conflict on nurses' job satisfaction via hindrance stress.Design/methodology/approachA questionnaire was answered by 224 nursing professionals working in public and private health institutions.FindingsA mediation–moderation model was estimated, and the results thereof indicated that work–family conflict has an indirect negative effect on satisfaction through the mediation mechanism of hindrance stress. Results also show that ethical leadership enhances this mediated relationship.Research limitations/implicationsThe cross-sectional data limited the generalization of results. Future research should develop longitudinal studies, which will allow for conclusions to be drawn with regard to inferences of causality.Practical implicationsIt is recommended that health organizations should develop ethical training for their leaders. By doing so, they will address two issues: the conciliation between work and family as well as the reduction of job stress, which will subsequently improve job satisfaction.Originality/valueThe originality of this empirical study lies in the exploration of the moderating role of ethical leadership in the indirect effect of work–family conflict, which, in turn, impacts on job satisfaction via the hindrance stress experienced by nurses. The study is innovative in the sense that it seeks to gain a greater understanding of the moderation–mediation mechanisms of the variables under study.

Rationale: Obstructive sleep apnea is recurrent upper airway obstruction caused by a loss of upper airway muscle tone during sleep. The main goal of our study was to determine if designer receptors exclusively activated by designer drugs (DREADD) could be used to activate the genioglossus muscle as a potential novel treatment strategy for sleep apnea. We have previously shown that the prototypical DREADD ligand clozapine-N-oxide increased pharyngeal diameter in mice expressing DREADD in the hypoglossal nucleus. However, the need for direct brainstem viral injections and clozapine-N-oxide toxicity diminished translational potential of this approach, and breathing during sleep was not examined.Objectives: Here, we took advantage of our model of sleep-disordered breathing in diet-induced obese mice, retrograde properties of the adeno-associated virus serotype 9 (AAV9) viral vector, and the novel DREADD ligand J60.Methods: We administered AAV9-hSyn-hM3(Gq)-mCherry or control AAV9 into the genioglossus muscle of diet-induced obese mice and examined the effect of J60 on genioglossus activity, pharyngeal patency, and breathing during sleep.Measurements and Main Results: Compared with control, J60 increased genioglossus tonic activity by greater than sixfold and tongue uptake of 2-deoxy-2-[18F]fluoro-d-glucose by 1.5-fold. J60 increased pharyngeal patency and relieved upper airway obstruction during non-REM sleep.Conclusions: We conclude that following intralingual administration of AAV9-DREADD, J60 can activate the genioglossus muscle and improve pharyngeal patency and breathing during sleep.

PurposeThe purpose of this paper is to explore the mediating effect of the work–family conflict in the relation between ethical leadership and job satisfaction.Design/methodology/approachA questionnaire was answered by 234 nursing professionals working in Portuguese public health institutions.FindingsRegression analyses indicate that there is a positive relationship between ethical leadership and satisfaction and a negative relationship between ethical leadership and the nurse's work–family conflict. Furthermore, it was revealed that the work–family conflict mediated (partially) the relationship between ethical leadership and job satisfaction.Research limitations/implicationsEthical leadership was measured by assessing the nurses' perceptions of their leaders' character. The cross-sectional data limited the possibility of establishing the causality of the study variables, where the generalization of results was not possible due to the fact that data were obtained in public health institutions alone.Practical implicationsConsidering that ethics precede good relations between the manager and collaborator, it is recommended that organizations develop ethical training for their leaders, more specifically in the case of head nurses.Originality/valueThe added value of this empirical study lies in the mediated role of the work–family conflict in the analysis of the relationship between ethical leadership and job satisfaction.

A cirrose hepática é condição de grande impacto na saúde mundial, e persiste entre as dez maiores causas de morte em países desenvolvidos, com 38,170 mortes atribuíveis à cirrose nos Estados Unidos no ano de 2014. Estudo brasileiro diagnosticou desnutrição em 75% de pacientes com doença hepática avançada, com prevalência de 46% entre pacientes classificados como Child-Pugh A, 84% no grupo Child-Pugh B e 95% no grupo Child-Pugh C. O mau estado nutricional na CH é fator reconhecidamente associado a pior prognóstico e maior incidência de complicações. O conceito de sarcopenia difere do de desnutrição, embora haja considerável sobreposição entre ambos. A prevalência da sarcopenia aumenta com a idade e se aproxima de 5-13% durante a sexta e a sétima década de vida. Tal síndrome está associada a maior risco de comorbidades, imobilismo, piora da qualidade de vida e mortalidade. Pacientes com CH e ascite apresentam distensão peritoneal, e frequentemente desenvolvem hérnias em áreas mais fracas da parede abdominal. Devido à frequente presença concomitante de sarcopenia, desnutrição e hérnias abdominais em pacientes cirróticos, o estudo avaliou a frequência dessas condições em amostra de pacientes ambulatoriais e se há presença de relação estatisticamente significativa entre status nutricional e hérnia. O estudo encontrou frequência de 19% de sarcopenia e 46% de desnutrição, sem evidenciar relação significativa do ponto de vista estatístico entre essas condições clínicas e hérnia abdominal Hepatic cirrhosis is a major global health condition and persists among the ten leading causes of death in developed countries, with 38,170 deaths attributable to cirrhosis in the United States in 2014. A Brazilian study diagnosed malnutrition in 75% of patients with advanced liver disease, with a prevalence of 46% among patients classified as Child-Pugh A, 84% in the Child-Pugh B group and 95% in the Child-Pugh C group. Poor nutritional status in CH is a known factor associated with worse prognosis and higher incidence of complications. The concept of sarcopenia differs from that of malnutrition, although there is considerable overlap between the two. The prevalence of sarcopenia increases with age and approaches 5-13% during the sixth and seventh decade of life. This syndrome is associated with an increased risk of comorbidities, immobility, worsening of quality of life and mortality. Patients with HC and ascites may present peritoneal distension, and often develop hernias in weaker areas of the abdominal wall. Due to the frequent concomitant presence of sarcopenia, malnutrition and abdominal hernias in cirrhotic patients, the study evaluated the frequency of these conditions in a sample of outpatients and whether there is a statistically significant relationship between nutritional status and hernia. The study found a frequency of 19% sarcopenia and 46% of malnutrition, without showing a significant statistical relationship between these clinical conditions and abdominal hernia

604501-EPP-1-2018-1-ES-EPPKA3-IPI-SOC-IN In this paper, we draw on a conceptual framework of five key categories of risk to Early Leaving (EL) to analyse and compare ‘Structural Factors’ (SF) and ‘Institutional Factors’ (IF) of risk in a region in England and in Portugal. We draw on data from an EU-funded project, which explored risks and support strategies to EL across five European countries. Our two-country comparison involved analysis of 59 semi-structured interviews and focus groups with 209 educational stakeholders across 18 educational settings. We discuss key similarities and differences across the data, reflecting on plausible factors these are attributed to. SF was the most discussed risk category in England but not in Portugal, albeit largely for the same reason: that they are difficult to address, relating to rigid and entrenched educational systems and policies, socio-economic disadvantage and inequalities. Educators were cognisant of how SF hinders educational institutions and stakeholders from addressing EL, given the lack of freedom and flexibility they engender. Young people focused more on how the IF of schooling are impacting EL. We explore the implications of our findings for EL risk and offer recommendations that will be of interest to a range of educational stakeholders, including policymakers, educators and employers. info:eu-repo/semantics/publishedVersion

Obstructive sleep apnea (OSA) is recurrent obstruction of the upper airway due to the loss of upper airway muscle tone during sleep. OSA is highly prevalent, especially in obesity. There is no pharmacotherapy for OSA. Previous studies have demonstrated the role of leptin, an adipose-tissue-produced hormone, as a potent respiratory stimulant. Leptin signaling via a long functional isoform of leptin receptor, LEPRb, in the nucleus of the solitary tract (NTS), has been implicated in control of breathing. We hypothesized that leptin acts on LEPRb positive neurons in the NTS to increase ventilation and maintain upper airway patency during sleep in obese mice. We expressed designer receptors exclusively activated by designer drugs (DREADD) selectively in the LEPRb positive neurons of the NTS of Leprb-Cre-GFP mice with diet-induced obesity (DIO) and examined the effect of DREADD ligand, J60, on tongue muscle activity and breathing during sleep. J60 was a potent activator of LEPRb positive NTS neurons, but did not stimulate breathing or upper airway muscles during NREM and REM sleep. We conclude that, in DIO mice, the stimulating effects of leptin on breathing during sleep are independent of LEPRb signaling in the NTS.

Rationale: North America is currently facing an opioid epidemics. Over 130 Americans die every day from an opioid overdose and the COVID-19 pandemic has increased opioid usage and opioid-related deaths. Respiratory depression is the main cause of morbidity and mortality from opioids. We have previously shown that leptin acts as respiratory stimulant and prevents opioid-induced respiratory depression and upper airway obstruction without reducing analgesia. In this study, we aimed to examine if intranasal (IN) administration of leptin effectively delivers leptin to the brain and prevents opioid-related deaths. Methods: To determine leptin delivery to the brain male C57BL/6J mice, were treated with IN leptin at 1.2 mg/kg in 1% BSA (n=5) or 1% BSA (n=5) and sacrificed 20 min later. Brains were harvested, the olfactory bulbs, medullas and hypotalami were isolated, quick frozen and stored at-80°C. For leptin level measurements, brain tissue was homogenized, protease inhibitors and protein concentrations were determined and ELISA was performed. To determine the survival probability, male C57BL/6J mice, 12 weeks of age, received IN leptin (n=26) or vehicle (n=25) at 1.2 mg/kg and 30 minutes later received a bolus of intraperitoneal (IP) morphine at 400mg/kg. Mice were video monitored for 24 hours and survival time after morphine injection was recorded Results: IN leptin significantly increased leptin levels in the olfactory bulb (from 0 ng/mL to 7.53 ng/mL, p = 0.004) and medulla (from 0.008 ng/mL to 0.107 ng/mL) and presented a trend in the hypothalamus (from 0.018 ng/mL to 0.291 ng/mL, p = 0.1). Mice that received IN leptin had a higher survival rate when compared to mice that received vehicle (69% vs 92%, p = 0.044, Figure). Conclusion: We demonstrated that the intranasal route is effective for the delivery of leptin to the brain and that leptin reduced opioid-induced mortality. (Table Presented).

The main aim of this study is to analyze the impact of green human resource management on organizational citizenship behavior through the mediating role of organizational identification and job satisfaction in Portuguese companies certified by ISO 14001. A survey was applied to a sample of 120 collaborators who work in ISO 14001 certified Portuguese industrial companies. Results indicate that there is a mediation model, which uses the effect of job satisfaction in the relationship between green human resource management and its impact on organizational citizenship behavior. This study proves to be important when understanding the individual effects caused by a green human resource management system.

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The use of the Internet and social networks have increased dramatically during the COVID quarantine mainly because several activities were moved online. In education, numerous stakeholders stayed at home and their academic plans were modified and adapted to an entire virtual environment. This was the case of a live event (Science Cafe) whose purpose was to disseminate knowledge through Facebook and YouTube. Thus, this study aimed at verifying if there was knowledge construction in social networks through user interactions by using 1,083 comments posted by the audience. Comments were coded according to validated frameworks for language taxonomy and collaborative knowledge construction. Results show that the predominant interaction is that in which viewers pose questions to speakers. Our analyses also revealed that attendees hardly reached the highest levels of knowledge construction through unguided interaction. Often, user interactions went beyond emotional expressions towards evaluation and therefore, could reach a higher level of knowledge construction. This study shows that social networks may offer informal spaces for deliberation and collaborative interaction with the potential to support learning if guided properly. This research aims to contribute empirical evidence to the growing body of literature that online interactions in informal environments may provide productive learning.

We live in a changing world, where the role of educational institutions is being transformed. The available technologies allow new conditions to access learning; however, as they evolve very fast, sometimes it is challenging to track this evolution regarding the changes that entail to all of us, which may contribute to a digital divide if it is not well addressed. This chapter intends to present distance education as a reliable modality to include students in higher education. For that, it presents the universal design for learning and its crucial role to make inclusive virtual learning environments. Like every modality that exists, there are some challenges that need to be addressed, as well as some benefits that allow to see the potential of this modality to learning, overcoming some adversities that may occur, like the lack of time to go to face-to-face education or even the need of social isolation by risk of getting a disease. info:eu-repo/semantics/publishedVersion

Study Objectives Obesity leads to obstructive sleep apnea (OSA), which is recurrent upper airway obstruction during sleep, and obesity hypoventilation syndrome (OHS), hypoventilation during sleep resulting in daytime hypercapnia. Impaired leptin signaling in the brain was implicated in both conditions, but mechanisms are unknown. We have previously shown that leptin stimulates breathing and treats OSA and OHS in leptin-deficient ob/ob mice and leptin-resistant diet-induced obese mice and that leptin’s respiratory effects may occur in the dorsomedial hypothalamus (DMH). We hypothesized that leptin receptor LepRb-deficient db/db mice have obesity hypoventilation and that restoration of leptin signaling in the DMH will increase ventilation during sleep in these animals. Methods We measured arterial blood gas in unanesthetized awake db/db mice. We subsequently infected these animals with Ad-LepRb or control Ad-mCherry virus into the DMH and measured ventilation during sleep as well as CO2 production after intracerebroventricular (ICV) infusions of phosphate-buffered saline or leptin. Results Awake db/db mice had elevated CO2 levels in the arterial blood. Ad-LepRb infection resulted in LepRb expression in the DMH neurons in a similar fashion to wildtype mice. In LepRb-DMH db/db mice, ICV leptin shortened REM sleep and increased inspiratory flow, tidal volume, and minute ventilation during NREM sleep without any effect on the quality of NREM sleep or CO2 production. Leptin had no effect on upper airway obstruction in these animals. Conclusion Leptin stimulates breathing and treats obesity hypoventilation acting on LepRb-positive neurons in the DMH.

Obesity can lead to recurrent upper airway obstruction (obstructive sleep apnea, OSA) during sleep as well as alveolar hypoventilation. We have previously shown that leptin stimulates breathing and treats OSA in leptin-deficientob/obmice and leptin-resistant diet-induced obese mice. Our previous data also suggest that leptin’s respiratory effects may occur in the dorsomedial hypothalamus (DMH). We selectively expressed leptin receptorLepRbin the DMH neurons of obeseLepRb-deficientdb/dbmice (LepRb-DMH mice), which hypoventilate at baseline, and showed that intracerebroventricular injection of leptin in these animals increased inspiratory flow, tidal volume and minute ventilation during NREM sleep without any effect on the quality of NREM sleep or CO2production. Leptin had no effect on upper airway obstruction inLepRb-DMHanimals. We conclude that leptin stimulates breathing and treats obesity related hypoventilation acting on LepRb-positive neurons in the DMH.

Respiratory depression is the main cause of morbidity and mortality associated with opioids. Obesity increases opioid-related mortality, which is mostly related to comorbid obstructive sleep apnea. Naloxone, a μ-opioid receptor blocker, is an effective antidote, but it reverses analgesia. Like humans with obesity, mice with diet-induced obesity hypoventilate during sleep and develop obstructive sleep apnea, which can be treated with intranasal leptin. We hypothesized that intranasal leptin reverses opioid-induced sleep-disordered breathing in obese mice without decreasing analgesia. To test this hypothesis, mice with diet-induced obesity were treated with morphine at 10 mg/kg subcutaneously and with leptin or placebo intranasally. Sleep and breathing were recorded by barometric plethysmography, and pain sensitivity was measured by the tail-flick test. Excitatory postsynaptic currents were recorded in vitro from hypoglossal motor neurons after the application of the μ-opioid receptor agonist [D-Ala(2), N-MePhe(4), Gly-ol]-enkephalin and leptin. Morphine dramatically increased the frequency of apneas and greatly increased the severity of hypoventilation and obstructive sleep apnea. Leptin decreased the frequency of apneas, improved obstructive sleep apnea, and completely reversed hypoventilation, whereas morphine analgesia was enhanced. Our in vitro studies demonstrated that [D-Ala(2), N-MePhe(4), Gly-ol]-enkephalin reduced the frequency of excitatory postsynaptic currents in hypoglossal motoneurons and that application of leptin restored excitatory synaptic neurotransmission. Our findings suggest that intranasal leptin may prevent opioid respiratory depression during sleep in patients with obesity receiving opioids without reducing analgesia.

O abandono escolar precoce, mais expressivo nos últimos anos da escolaridade obrigatória, é um dos desafios das agendas políticas europeias, afetando a inclusão educacional e a vida social e profissional do aluno. Este artigo expõe alguns resultados do projeto Europeu Orienta4YEL, nomeadamente os que decorrem do contexto português. Através de uma investigação qualitativa, procurou-se conhecer e interpretar o abandono escolar precoce e, em particular, as possibilidades de promoção do reingresso de jovens que frequentam cursos profissionais. Os dados foram recolhidos através de entrevistas, individuais e grupais, realizadas em sete escolas. As entrevistas individuais foram aplicadas aos elementos dos órgãos diretivos, e os grupos focais a 42 professores e 68 alunos. Através da análise de conteúdo, os resultados evidenciam a existência de fatores de ordem estrutural, institucional, social, familiar e pessoal que podem estimular o desejo dos jovens reingressarem no ensino. Das perspetivas dos participantes, destaca-se o papel atribuído à prevenção do abandono escolar precoce, a necessidade de adaptar os percursos formativos de acordo com as especificidades e necessidades dos ex-alunos, promover uma maior aproximação à escola através de diálogo e apoio individualizado e fomentar o envolvimento da família no processo de tomada de decisão de retorno dos jovens à vida escolar.

Introduction Obstructive sleep apnea (OSA) is characterized by recurrent periods of upper airway obstruction. The prevalence of OSA exceeds 50% in obese individuals and in 10–20% of obese patients OSA coexists with obesity hypoventilation syndrome (OHS) defined as daytime hypercapnia and hypoventilation during sleep attributed to the depressed control of breathing. There is no effective pharmacotherapy for OSA and OHS. Leptin is a potent respiratory stimulant and a potential therapeutic candidate. However, diet-induced obesity (DIO) results in reduced permeability of the blood-brain barrier (BBB) for leptin. Previous studies have shown that the BBB can be penetrated by exosomes, natural nanoparticles that can be used as drug delivery vehicles. In this study, we aimed to determine if exosomes overcome the BBB and treat OSA and OHS in DIO mice. Methods o examine the ability of exosomes to cross the BBB, male, lean (n=5) and DIO (n=5) C57BL/6J mice were injected with fluorescent exosomes or saline into the lateral tail vein. After 4h fluorescent exosomes biodistribution was evaluated by an in vitro imaging system (IVIS). Saline injected mice images were used for background adjustment. A separate subgroup of male, DIO (n=10) and lean (n=10) mice were headmounted with EEG and nuchal EMG leads. Sleep studies were performed in a plethysmography chamber and mice received saline, empty exosomes, free leptin, or leptin-loaded exosomes in a crossover manner. Results Exosomes were successfully delivered to the brain and the transport across the BBB was more efficient in DIO mice with 2-times greater relative fluorescence units measured in DIO when compared to lean mice (p Conclusion We demonstrated that exosomes overcome the BBB and that leptin-loaded exosomes treat OSA and OHS in DIO mice. Support (if any) R01HL 128970, R01HL 138932, R61 HL156240, U18 DA052301, FAPESP 2018/08758-3

The purpose of this study is to analyse how the access to structures of empowerment by teachers in primary and secondary education impacts on their trust of the headmaster of the school management board. Using the theoretical framework of empowerment and trust in the context of companies, one adapted the constructs of these scales to the reality of primary and secondary schools. A questionnaire was presented to 112 teachers in order to evaluate the impact of empowerment structures on their trust of the leader. The results obtained indicate that a context of empowering work is significantly predictive of trust in the school’s headmaster. The teaching staff who realize that they have access to empowerment structures (power, resources, opportunities, support and information) and feel that they can exercise some influence on the organization of their workplace are in a better position to trust the headmaster. The results are discussed and the contributions of the study to theory and to school administration are presented.

BjcuL is a C-type lectin isolated from Bothrops jararacussu snake venom with specificity for binding β-d-galactose units. BjcuL is not toxic to human peripheral blood mononuclear cells (PBMCs), but it inhibits PBMC proliferation and stimulates these cells to produce superoxide anions and hydrogen peroxide primarily via lymphocyte stimulation; it does not stimulate the production of nitric oxide and PGE

O ProLearn4ALL – Maletas Pedagógicas para TODOS é um projeto que tem como principal propósito a criação de recursos lúdico-pedagógicos acessíveis a crianças do 1º Ciclo do Ensino Básico, com o intuito de as sensibilizar para a diferença e, em particular, para os quatro domínios da deficiência (intelectual, motora, auditiva e visual). Este tem a particularidade de incluir, na sua equipa de trabalho, diversos elementos, não só docentes e investigadores, mas também estudantes de diferentes níveis do Ensino Superior (Cursos Técnicos Superiores Profissionais, Licenciaturas e Mestrados) que são confrontados com uma sequência de questões para as quais têm de encontrar respostas e soluções válidas. A aprendizagem e investigação baseada em problemas tornaram-se, portanto, as estratégias seguidas ao longo deste trabalho, estratégias estas que implicam uma avaliação sistemática. A avaliação de todo o processo, desde a fase de planificação à implementação, encontra-se descrita neste artigo, refletindo-se sobre as opções tomadas ao nível dos instrumentos e técnicas a aplicar. Estando este projeto em desenvolvimento, espera-se que os resultados desta avaliação permitam compreender as repercussões do projeto nos conhecimentos e atitudes dos estudantes do Ensino Superior, mas também o impacte do mesmo em turmas do Ensino Básico, que incluem crianças com e sem deficiência, e dos seus respetivos professores. info:eu-repo/semantics/publishedVersion

The present disclosure generally relates to compositions and methods of treating opioid-induced respiratory depression in a subject in need thereof, the method comprising administering leptin.

BjcuL is a C-type lectin with specificity for the binding of β-D-galactose units isolated from Bothrops jararacussu venom. It triggers cellular infiltration in post capillary venules, increases edema and vascular permeability inmurine models, contributes to in vitro neutrophil activation and modulates macrophage functional activation towards an M1 state. The purpose of this study was to investigate the effect of BjcuL on human peripheral blood mononuclear cells (PBMCs) activation with a focus on PBMCs proliferation and inflammatory mediators release. Results showed that BjcuL is not toxic to PBMCs, that BjcuL inhibits PBMCs proliferation and that it stimulates PBMCs to produce superoxide anion and hydrogen peroxide, primarily via lymphocyte stimulation, but does not stimulate the production of nitric oxide and PGE2. These results demonstrate that BjcuL has an immunomodulatory effect on PBMCs. Further studies are needed to confirm the immunomodulatory effect of BjcuL, to elucidate the molecular mechanisms of action responsible for its effects and to determine its potential application as an immunopharmacological and biotechnological tool.

The main aim of this study is to explore the underlying factors of the subordinate-supervisor trust relationship and to analyze its impact on the three components of organizational commitment. Data was collected from 192 R&D professionals working at 10 different ICT Portuguese industrial companies. The study confirms the three factors of integrity, benevolence and ability as the basis for the perceived trustworthiness of supervisors by subordinates. In addition, results show that the trust a team member places on his/her leader depends predominantly on his/her perception of integrity. The authors conclude that even in the technological field, where the prevalence of a technical competence-based trust relationship was expected, integrity emerged as important element in the subordinate's trust of his/her supervisor. Integrity was found to correlate significantly with affective, normative and calculative commitment. The authors' findings suggest that organizations can “manage” commitment in ICT industrial enterprises through fostering the perception of integrity by subordinates.

Obstructive sleep apnea (OSA) is a highly prevalent disease characterized by recurrent closure of the upper airway during sleep. It has a complex pathophysiology involving four main phenotypes. An abnormal upper airway anatomy is the key factor that predisposes to sleep-related collapse of the pharynx, but it may not be sufficient for OSA development. Non-anatomical traits, including (1) a compromised neuromuscular response of the upper airway to obstruction, (2) an unstable respiratory control (high loop gain), and (3) a low arousal threshold, predict the development of OSA in association with anatomical abnormalities. Current therapies for OSA, such as continuous positive airway pressure (CPAP) and oral appliances, have poor adherence or variable efficacy among patients. The search for novel therapeutic approaches for OSA, including pharmacological agents, has been pursued over the past years. New insights into OSA pharmacotherapy have been provided by preclinical studies, which highlight the importance of appropriate use of animal models of OSA, their applicability, and limitations. In the present review, we discuss potential pharmacological targets for OSA discovered using animal models.