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98 results on '"CHIGUSA, Yuichi"'

52. Utilization of ELISA Using Thioredoxin Peroxidase-1 and Tandem Repeat Proteins for Diagnosis of Schistosoma japonicum Infection among Water Buffaloes

Author

Angeles, Jose Ma. M., primary, Goto, Yasuyuki, additional, Kirinoki, Masashi, additional, Asada, Masahito, additional, Leonardo, Lydia R., additional, Rivera, Pilarita T., additional, Villacorte, Elena A., additional, Inoue, Noboru, additional, Chigusa, Yuichi, additional, and Kawazu, Shin-ichiro, additional

Published
2012
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59. Fungi can be a useful forensic tool

Author

Hitosugi, Masahito, primary, Ishii, Kiyoshi, additional, Yaguchi, Takashi, additional, Chigusa, Yuichi, additional, Kurosu, Akira, additional, Kido, Masahito, additional, Nagai, Toshiaki, additional, and Tokudome, Shogo, additional

Published
2006
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92. Molecular characterization of sympatrically distributed Neotricula aperta-like snails in the Mekong River, Kratie, Cambodia.

Author

Wang C, Saijuntha W, Kirinoki M, Hayashi N, Chigusa Y, Muth S, Meng CC, Ai Y, and Agatsuma T

Subjects
Animals, Cambodia, Rivers, Electron Transport Complex IV genetics, Haplotypes, Phylogeny, Snails classification, Snails genetics
Abstract

Fifty-six samples of Neotricula aperta-like snails were collected from six locations in Cambodia. Their mitochondrial cytochrome c oxidase subunit 1 (cox1) sequences were examined using haplotype network and neighbor-joining (NJ) tree analysis. Twenty-seven haplotypes (H1-H27) were observed and were divided into two different groups/lineages. Of 27, 17 haplotypes (H11-H27) were clustered with the reference samples of the γ-race N. aperta. The remaining 10 haplotypes (H1-H10) were clustered in a separate group/lineage, differing from the reference samples of the α-, β-, and γ-race N. aperta, suggesting a new lineage belonging the genus Neotricula. Our results show that both the γ-race and a new lineage were sympatrically present approximately 60 km upstream of the Mekong River near the Kratie port, Cambodia. Further morphological and molecular studies are required to confirm the taxonomic status of this new, unidentified lineage.

Published
2016
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94. Efficacy of sodium metaperiodate (SMP)-ELISA for the serodiagnosis of schistosomiasis mekongi.

Author

Kirinoki M, Chigusa Y, Ohmae H, Sinuon M, Socheat D, Matsumoto J, Kitikoon V, and Matsuda H

Subjects
Animals, Antigens, Helminth chemistry, Cambodia epidemiology, Endemic Diseases, Enzyme-Linked Immunosorbent Assay, Feces parasitology, Humans, Schistosomiasis drug therapy, Schistosomiasis epidemiology, Schistosomiasis prevention & control, Sensitivity and Specificity, Serologic Tests methods, Periodic Acid chemistry, Schistosoma isolation & purification, Schistosomiasis diagnosis
Abstract

Schistosomiasis mekongi is an important public health issue in endemic countries. In this study, we evaluated an indirect immunodiagnostic ELISA method using Schistosoma mekongi soluble egg antigen. Sodium metaperiodate (SMP)-ELISA was utilized in order to remove the glycosylated epitopes responsible for false positive reactions and the results using this method were compared with those using conventional ELISA (conv-ELISA). Forty-two serum samples from schistosomiasis mekongi egg-positive patients and 100 serum samples from schistosomiasis-negative Cambodian subjects were tested using both ELISA methods. The ranges of ELISA values for positive and negative sera were distinct on SMP-ELISA, but the ranges of the two groups of sera overlapped on conv-ELISA. Therefore, diagnostic criteria may be established based on the highest ELISA value on negative sera and the lowest ELISA value on positive sera. In the present study, both the sensitivity and specificity of SMP-ELISA reached 100% using the criteria in which an ELISA value > or = 0.2 was positive.

Published
2011

95. Development of the rapid and simple elisa (whole blood-ELISA) using coconut water-tween as a wash solution for whole blood sample from Schistosoma japonicum-infected rabbit and human.

Author

Hirose Y, Lipayon IL, Kirinoki M, Chigusa Y, and Matsuda H

Subjects
Animals, Humans, In Vitro Techniques, Mass Screening methods, Rabbits, Schistosoma japonicum enzymology, Schistosomiasis blood, Schistosomiasis enzymology, Serologic Tests methods, Solutions, Cocos, Enzyme-Linked Immunosorbent Assay methods, Schistosoma japonicum isolation & purification, Schistosomiasis diagnosis
Abstract

PBS-Tween as a wash solution, prepared with distilled water, is used in ELISA. In areas where schistosomiasis is endemic, however, distilled water is hard to come by. We have modified a WHOLE BLOOD-ELISA test to use coconut water-Tween as a wash solution, because coconut water is easy to come by and cheap in the tropics. We applied the test to whole blood samples from rabbits and humans infected with Schistosoma japonicum. This modified WHOLE BLOOD-ELISA was confirmed to be a rapid, simple, and cost-effective method.

Published
2005

96. A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits.

Author

Hirose Y, Kirinoki M, Chigusa Y, and Matsuda H

Subjects
Animals, Mass Screening methods, Rabbits, Time Factors, Antigens, Helminth blood, Enzyme-Linked Immunosorbent Assay methods, Schistosoma japonicum immunology, Schistosomiasis diagnosis
Abstract

A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits was compared with a conventional ELISA. This whole-blood ELISA has advantages. The volume of crude egg antigens, whole blood samples, and conjugates was only 0.05 ml. The incubation time was shortened to 5 minutes. Wells were washed three to five times with PBS-Tween after each procedure. Optical density values were measured in 10 minutes after transfer of 0.1 ml of substrate. Constant temperature was not necessary. The entire procedure took only 20-30 minutes.

Published
2005

97. Development of the rapid and simplified ELISA (WHOLE BLOOD-ELISA) using samples of Schistosoma japonicum-infected human whole blood.

Author

Hirose Y, Kirinoki M, Lipayon IL, Blas BL, Chigusa Y, and Matsuda H

Subjects
Adolescent, Adult, Aged, Animals, Child, Child, Preschool, Female, Humans, Male, Mass Screening, Middle Aged, Schistosomiasis blood, Antigens, Helminth blood, Enzyme-Linked Immunosorbent Assay methods, Schistosoma japonicum immunology, Schistosomiasis diagnosis
Abstract

An ELISA technique was developed using samples of Schistosoma japonicum-infected human whole blood based on the conventional ELISA. In this study, the following were demonstrated. 1) Whole blood samples could be used. 2) The volume of whole blood and conjugate could be reduced to 0.05 ml. 3) The incubation time was shortened to 5 minutes. 4) The optical density could be measured at 10 minutes after transferring the substrate and the volume was reduced to 0.1 ml. 5) It did not require a fixed temperature setting. 6) The operation time was as short as 20 to 30 minutes. 7) The optical density values were almost the same as the conventional ELISA and were not influenced by other common intestinal helminthic infections. 8) The observed variations from day to day including effects of sampling in stool examination were negated by the results of this ELISA technique. 9) Based on correlation with stool examination results, criteria can be formulated in which optical density values of 0.3 and above as positive, 0.1 to less than 0.3 as doubtful, and less than 0.1 as negative. Whenever an immunological field survey is necessary, before and after a selective or a mass treatment control program, this WHOLE BLOOD-ELISA, which was shown to be rapid and simple, is recommended.

Published
2005

98. Immunoblot analysis of Schistosoma japonicum egg antigens with sera from patients with acute and chronic schistosomiasis japonica.

Author

Kirinoki M, Hu M, Yokoi H, Chigusa Y, and Matsuda H

Subjects
Acute Disease, Adolescent, Adult, Animals, Antibodies, Helminth blood, Child, China, Chronic Disease, Humans, Immunoglobulin G blood, Middle Aged, Ovum immunology, Schistosomiasis japonica blood, Schistosomiasis japonica immunology, Sensitivity and Specificity, Antigens, Helminth, Immunoblotting methods, Schistosoma japonicum immunology, Schistosomiasis japonica diagnosis
Abstract

Humoral immune responses of IgG, IgM, IgA, IgE and IgG subclass antibodies to Schistosoma japonicum egg antigens were determined by immunoblotting with serum samples from individuals in China with acute (n=24) or chronic (n=35) schistosomiasis. In general, IgM, IgA, and IgE in sera from acute patients exhibited strong binding to antigens but binding was much weaker in chronic cases. Reaction of IgG4 of chronic cases was stronger than that of IgG4 of acute cases. The recognition profile of each antibody isotype in sera was analyzed for 11 major antigen molecules (antigens with apparent molecular weights of 82, 76, 61, 57, 53, 46, 40, 32, 27, 10 and less than 6.5 kDa). Except for the 10 kDa molecule, they were well-recognized by IgA and IgE in sera of acute cases. In other combinations of antibody class and clinical phase, recognition patterns against these molecules differed among individuals. Notably, the 10 kDa molecule was specifically recognized by total IgG and IgG4 in sera from most of the chronic patients, but in sera from only one acute case. This result suggests that the 10 kDa molecule is one of the major target antigens of IgG4 and may be useful as a marker antigen to characterize the clinical phases of S. japonicum infection.

Published
2003

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