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Contamination of Pacific oysters, Crassostrea gigas, by human norovirus (HuNoV) is a major constraint to sustainable shellfish farming in coastal waters of the Northeast Pacific. HuNoV is not a marine virus and must originate from a human source. A barrier to effective management is a paucity of data regarding HuNoV dispersal in the marine environment. The main objective of this study was to identify the spatial distribution and persistence of HuNoV in an active shellfish farming region in the Northeast Pacific. Market-size C. gigas were sequentially deployed for two-week intervals at 12 sites during the 2020 winter risk period from January to April. Detection of HuNoV quantification was performed by reverse transcription real-time PCR (RTqPCR) according to method ISO 15216-1:2017, with modifications. RTqPCR did not detect GI HuNoV. The estimated prevalence of GII HuNoV in oyster digestive tissue was 0.8 ± 0.2%. Spatiotemporal analysis revealed that contamination of oysters with GII HuNoV changed through time and space during the surveillance period. A single cluster of oysters contaminated with GII.2 HuNoV was detected in a small craft harbor on 23 April. There was no significant increase in the proportion of positive pools in the next nearest sampling station, indicating that HuNoV is likely to disperse less than 7 km from this non-point source of contamination. Results from this study indicate that HuNoV contamination of coastal waters from non-point sources, such as small craft harbors and urban settings, can pose a significant localised risk to shellfish farming operations in the region.

Do chronic fluoxetine treatments reduced footshock-induced posttraumatic stress disorder (PTSD) symptoms, including fear and comorbid depression, in the situational reminder phase? Moreover, are the subareas of the medial prefrontal cortex (mPFC), including the cingulate cortex 1 (Cg1), prelimbic cortex (PrL), infralimbic cortex (IL), and basolateral amygdala (BLA), involved in the fluoxetine amelioration of PTSD symptoms? These two crucial issues were addressed in the present study. All mice were injected with chronic fluoxetine or normal saline treatments for the adaptation (14 days), footshock fear conditioning (1 day), and situational reminder (3 days) phases. After adaptation, the mice were subjected to footshock (2 mA, 10 seconds) or nonfootshock and stayed 2 min in a footshock box for 2 min for fear conditioning. Later, they were placed in the footshock box for 2 min in the situational reminder phase. In the final session of the situational reminder phase, a forced swimming test (FST) and immunohistochemical staining were conducted. The results indicated that footshock induced fear and comorbid depression. Meanwhile, chronic fluoxetine treatments reduced fear and depression behaviors. The Cg1, PrL, IL, and BLA were seemingly to increase c-Fos expression after footshock-induced PTSD symptoms in the situational reminder phase. The fluoxetine treatments reduced only the BLA’s c-Fos expression. The findings suggest that BLA contributes to the fluoxetine amelioration of PTSD symptoms; however, the mPFC, including the Cg1, PrL, and IL, did not mediate PTSD symptoms’ amelioration stemming from fluoxetine. The present data might help us to further understand the neural mechanism of fluoxetine treatments in PTSD symptoms.

Abstract Activated T cells have been shown to be able to recirculate into the thymus from the periphery. The present study was aimed to elucidate the functional consequences of thymic homing of activated T cells upon developing thymocytes and thymic epithelial cells (TEC). In the presence of activated T cells, especially CD4+ T cells, T cell development was found to be inhibited in thymic organ cultures with markedly reduced cellularity. Thymic transplantation demonstrated that the inhibitory effect was most likely due to a defective microenvironment. As the major component of the thymic stroma, the TEC compartment was severely disturbed after prolonged exposure to the activated T cells. In addition to reduced cell proliferation, TEC differentiation was heavily skewed to the mTEC lineage. Furthermore, we demonstrated that RANKL highly expressed by activated CD4+ T cells was primarily responsible for the detrimental effects. Presumably, excessive RANK signaling drove overproduction of mTECs and possibly exhaustion of epithelial progenitors, thereby facilitating the deterioration of the epithelial structures. These findings not only reveal a novel activity of activated T cells re-entering the thymus, but also provide a new perspective for understanding the mechanism underlying thymic involution.

Zipper hydraulic fracturing in multiple wells with long horizontal sections is a primary solution means to increase the shale gas production rate and efficiency and to reduce the cost in Southern Sichuan Basin. Microseismic based fracturing monitoring can be used for real-time imaging of hydraulic fractures, so it has been widely used to evaluate the fracturing effect of shale gas reservoirs and to direct the optimization and adjustment of fracturing parameters. In China, however, the microseismic fracturing monitoring on fracturing of shale gas reservoirs cannot be used to evaluate the fracturing results until the fracturing operation in the pad wells is completed according to the parameters which are designed prior to the fracturing monitoring. Its evaluation results can merely provide a guidance for the fracturing parameters of the next pad wells instead of the wells in operation. As a result, the real-time effect of microseismic fracturing monitoring is out of work. In view of this, the fractures induced by zipper hydraulic fracturing in multiple shale gas wells with long horizontal sections in the southern Sichuan Basin, was real-time imaged by using the combined technology of radially arranged microseismic surface monitoring and microseismic well monitoring on the basis of real-time positioning method. The fracturing results were assessed and used in real time for the optimization of prepad fluid parameter, perforation and temporary plugging additive releasing time, so as to effectively avoid repeated fracturing and uneven fracturing effects and improve fracturing stimulation effects. This method is applied in two well groups. It is shown that the average shale gas production rate is increased by 2–5 times. Furthermore, microseismic fracturing real-time monitoring plays a vital role in real-time evaluation of fracturing effect and real-time optimization of fracturing parameters, so it can be used as the reference and should be popularized further.

While all reversible receptor-targeting radioligands for positron emission tomography (PET) can be displaced by competition with an antagonist at the receptor, many radiotracers show limited occupancies using agonists even at high doses. [11C]Raclopride, a D2/D3 receptor radiotracer with rapid kinetics, can identify the direction of changes in the neurotransmitter dopamine, but quantitative interpretation of the relationship between dopamine levels and radiotracer binding has proven elusive. Agonist-induced receptor desensitization and internalization, a homeostatic mechanism to downregulate neurotransmitter-mediated function, can shift radioligand-receptor binding affinity and confound PET interpretations of receptor occupancy. In this study, we compared occupancies induced by amphetamine (AMP) in drug-naive wild-type (WT) and internalization-compromised β-arrestin-2 knockout (KO) mice using a within-scan drug infusion to modulate the kinetics of [11C]raclopride. We additionally performed studies at 3 h following AMP pretreatment, with the hypothesis that receptor internalization should markedly attenuate occupancy on the second challenge, because dopamine cannot access internalized receptors. Without prior AMP treatment, WT mice exhibited somewhat larger binding potential than KO mice but similar AMP-induced occupancy. At 3 h after AMP treatment, WT mice exhibited binding potentials that were 15 % lower than KO mice. At this time point, occupancy was preserved in KO mice but suppressed by 60 % in WT animals, consistent with a model in which most receptors contributing to binding potential in WT animals were not functional. These results demonstrate that arrestin-mediated receptor desensitization and internalization produce large effects in PET [11C]raclopride occupancy studies using agonist challenges.

Social Sciences and Humanities research is divided across a wide array of disciplines, sub- disciplines and languages. While this specialisation makes it possible to investigate the extensive variety of SSH topics, it also leads to a fragmentation that prevents SSH research from reaching its full potential. Use and reuse of research is suboptimal, interdisciplinary collaboration possibilities are often missed partially because of missing standards and referential keys between disciplines. Often, the reuse of data may paradoxically complicate a relevant sorting of data and a trust relationship between researchers. As a result, societal, economic and academic impacts are limited. Conceptually, there is a wealth of transdisciplinary collaborations, but in practice there is a need to help researchers and research institutions to connect them and support them, to prepare the research data for these overarching approaches and to make them findable and usable. The TRIPLE (Targeting Researchers through Innovative Practices and Linked Exploration) project is a practical answer to the above issues, as it aims at designing and developing the European discovery platform dedicated to Social Sciences and Humanities resources. Funded under the European Commission program INFRAEOSC-02-2019 “Prototyping new innovative services”, thanks to a consortium of 19 partners, TRIPLE will develop a full multilingual and multicultural solution for the discovery and the reuse of Social Sciences and Humanities resources. The project started in October 2019 for a duration of 42 months thanks to European funding of 5.6 million €.

Objective: Establishing a practical procedure to generate induced pluripotent stem cells (iPSCs) and induced neural stem cells (iNSCs) from human urine cells (UCs). In this report, we optimized a non-integrative protocol to generate patient-specific iPSC and iNSC lines with high reprogramming efficiency.Methods: UCs were electroporated with the pEP4-EO2S-ET2K and pEP4-M2L plasmids containing the OCT4, SOX2, KLF4, SV40LT, c-MYC, and LIN28 genes, and then cultured with N2B27 medium plus four small molecule compounds (A83-01, PD0325901, Thiazovivin, and CHIR99021). When iPSC or iNSC clones emerged, the medium was replaced with mTeSR1 or neural growth medium. Morphological changes were seen at day 4–7. After day 10, the clones were picked up when the clone diameter exceeded 1 mm.Results: iPSCs and iNSCs were successfully derived from UCs with up to 80 clones/well. These iPSCs and iNSCs showed typical hESC or NSC morphology and were self-renewable. The iPSCs had pluripotency to differentiate into the three germinal layers and displayed high levels of expression of pluripotency markers SOX2, NANOG, OCT4, SSEA-4, TRA-1-60, TRA-1-81, and alkaline phosphatase (AP). They maintained normal karyotype and had no transgene expression or genomic integration. The iNSCs were positive for NSC markers NESTIN, PAX6, SOX2, and OLIG2.Conclusion: The optimized protocol is an easy and fast procedure to yield both iPSC and iNSC lines from a convenient source of human urine in a single experiment.

Taiwan has been promoting its national health insurance (NHI), which provides people with appropriate medical resources; however, health insurance education is missing from schools, and teachers lack relevant teaching abilities. This study describes the development of the health insurance education empowerment program (HIEEP) to promote effective teaching on educators. A two-group parallel-controlled trial was conducted with health education teachers, in which participants learned to discuss its importance through demonstration and practice. The questionnaire measured their teaching effectiveness in HIEEP using a baseline measurement and a one-week follow-up measurement. Of 196 people that started the trial, 186 completed it. The intervention effects were evaluated using the Johnson-Neyman method. The results showed that in the experimental group, the program raised all teachers' variable scores, which evaluated health insurance meaning, teaching competence, self-determination, and impact. The empowerment program can provide teachers will allow for more effective health insurance education.

Existing air purifiers are mostly indoor air circulation, and most of them cannot achieve indoor and outdoor air circulation and provide fresh air to room. At the same time, the current air purifier filter on the market after absorbing a large amount of dust and other pollutants, if not replaced in time, is likely to cause secondary pollution. In response to these problems, this paper carried out an exploratory research that can achieve indoor and outdoor air purification device. According to the clean air volume of HEPA filter, the pressure of fan and the cross-sectional area of filter are determined, meanwhile, the air resistance of indoor and outdoor circulating air purifier, the effect of air blower on air purification efficiency and filter CADR are discussed in detail. According to the filtering characteristics of single-layer filter, a kind of indoor and outdoor circulating air purifier-single-layer filter type air purifier was designed. Among the air purifier, the PLC control circuit is used to achieve single-layer filter air purifier filter automatically replace.