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WOS: 000398862500009, PubMed ID: 28394499, ObjectivesTo examine the possible protective effect of quercetin (QT), which is well known for its antioxidant and protective effects in circumstances of oxidative stress, on urinary bladder tissue in a rat model of ischemia/reperfusion (I/R) injury, which is a known factor for the development of lower urinary tract dysfunction partly mediated by the generation of free radicals causing oxidative damage. MethodsThirty male Sprague-Dawley rats were subjected to I/R injury through clamping the abdominal aorta for 30 min and then allowing reperfusion for the next 60 min. Quercetin (20 mg/kg; subcutaneously) or vehicle were given before ischemia and just before reperfusion. Findings of the isometric contraction studies in the organ bath and of the histological examinations along with oxidative stress markers were evaluated in bladder tissues. ResultsIncreased malondialdehyde (MDA) levels and myeloperoxidase (MPO) activities and decreased glutathione (GSH) levels and superoxide dismutase (SOD) activities in the I/R group were reduced by QT treatment. In the I/R group, pro-apoptotic marker caspase-3 was increased and anti-apoptotic bcl-2 protein was decreased, while QT treatment significantly reversed these parameters. In the I/R group contractile responses of the bladder strips to carbachol were significantly lower than those of the control group, which were reversed by QT treatment. ConclusionQuercetin treatment protects bladder tissue contractility against acute I/R injury by decreasing oxidative stress and apoptosis induced by I/R.

Amaç: Kapasite olmuş spermin fertilizasyon kapasitesininzamanla üstsel olarak düştüğü bilinmesine rağmen bunun nedenihala araştırılmaktadır. Çalışmada amaç; uzamış inkübasyonunve sıcaklığın sperm fertilizasyon kapasitesi üzerinde etkisinihareketlilik ve oosit aktivasyon faktörü olarak kabul edilenfosfolipaz C-zeta (PLCZ) ile değerlendirmektir.Gereç ve Yöntem: On altı infertil erkek hastadan, ciddioligoasthenospermisi olmayan 11’inin semenlerine dansitegradyan yıkaması uygulandı. Ardından örnekler bölünerek 3 günoda sıcaklığında veya 37°C’de kültüre edildi. Örnekler hareketlilik,PLCZ boyanma yüzdesi ve yoğunluğu açısından günlük olarakdeğerlendirildi.Bulgular: Hem oda sıcaklığı, hem de vücut sıcaklığında herüç parametrede de zamanla azalma gözlendi. Özellikle günler arasıhareketlilikteki değişim ile PLCZ boyanan sperm yüzdesindekideğişim arasında güçlü bir korelasyon bulundu, ancak bukorelasyon zamanla azalmakta idi.Sonuç: İnkübasyon süresinin uzamasıyla, PLCZ boyanmaparametreleri ve hareketlilik arasında korelasyon görülmektedir.PLCZ boyanmasının semen analizi ile rutinde kullanımı, özellikleaçıklanamayan infertilite ve fertilizasyon başarısızlığı vakalarınınspermlerinin fertilizasyon kapasitesinin belirlenmesinde yararsağlayabilecek ve yardımcı üreme teknileri (YÜT) sikluslarındakibaşarıyı artırabilecektir., Objective: Fertilization capacity of capacitated sperm decreasesexponentially over time, but the reason is still under investigation.The aim of the study was to analyze the effect of prolongedincubation and temperature on sperm fertilization capacity withmotility and staining parameters of phospholipase C-zeta (PLCZ),which is considered to be the oocyte activation factor.Materials and Methods: Density gradient washingwas applied to semen of 11 infertile patients without severeoligoasthenospermia out of 16 patients. The samples were dividedand cultured either at room temperature or at 37°C for 3 days. Thespermatozoons were evaluated for motility, PLCZ staining andintensity daily.Results: All parameters decreased both at room and bodytemperature with increased incubation time. There was a strongcorrelation between the change in motility and in the percentageof PLCZ stained sperms, but this correlation decreased withincubation time.Conclusion: Prolonged incubation results show the correlationbetween PLCZ staining parameters and motility. Routine use ofPLCZ staining together with semen analysis, will be useful topredict fertilization capacity of the sperm especially for unexplainedinfertility and fertilization failure cases, and also can increase thesuccess of assisted reproductive technologies (ART) cycles.

WOS: 000337658400027, PubMed ID: 24967205, AIM: To compare bacterial biofilm colonization in lacrimal stents following external dacryocystorhinostomy (EX DCR), endoscopic dacryocystorhinostomy (EN-DCR), and transcanalicular dacryocystorhinostomy (TC-DCR) with multidiode laser. METHODS: This prospective study included 30 consecutive patients with nasolacrimal duct obstruction who underwent EXT-, EN-, or TC-DCR. Thirty removed lacrimal stent fragments and conjunctival samples were cultured. The lacrimal stent biofilms were examined by scanning electron microscopy (SEM). RESULTS: ElevEN-(36.7%) of the 30 lacrimal stent cultures were positive for aerobic bacteria (most commonly Staphylococcus spia'arrniokS and Pseudomonas aeruginosa). However anaerobic bacteria and fungi were not identified in the lacrimal stent cultures. Twenty sevEN-(90%) patients had biofilm positive lacrimal stents. The conjunctival culture positivity after the DCR, biofilm positivity on stents, the grade of biofilm colonization, and the presence of mucus and coccoid and rod shaped organisms did not significantly differ betweEN-any of the groups (P>0.05). However, a significant difference was found whEN-the SEM results were compared to the results of the lacrimal stent and conjunctival cultures (P, Institutional Review Board of Bagcilar Education and Research Hospital, Istanbul, TurkeyIstanbul Bagcilar Training & Research Hospital [1852], Supported by Institutional Review Board of Bagcilar Education and Research Hospital, Istanbul, Turkey (No.1852)