93 results on '"C. Polese"'
Search Results
52. Non-destructive Approach to Multilayer Objects: XRF Analysis of Gilt and Enamelled Metals of the Medieval Cross of Rosciolo
- Author
-
Irene Sabatini, Luigi Campanella, Marco Ferretti, C. Polese, and Clodoaldo Roldán García
- Subjects
Materials science ,Chemistry(all) ,Enamel paint ,enamels ,fundamental parameter ,gilt silver ,medieval processional cross ,multilayered materials ,x-ray fluorescence ,Metallurgy ,Gilding ,X-ray fluorescence ,General Medicine ,Fundamental parameter ,Enamels ,Gilt silver ,Multilayered materials ,visual_art ,Non destructive ,Chemical Engineering(all) ,Forensic engineering ,visual_art.visual_art_medium ,fundamental parameters ,Medieval processional cross - Abstract
The penetration of X-rays is usually a limitation to the application of XRF analysis to multilayered materials, however it is possible, by software tools based on the fundamental parameters method, to overcome this problem and estimate thickness and composition of each layer. The cross of Rosciolo is a significant example of multilayer object: it is made of a wood core with attached gilt silver sheets and decorated with enamels. Measurements were carried out in situ and the PyMCA software was employed. The values of gilding thickness, Au/Hg ratios and Cu content in the silver sheets, and enamel's composition are discussed.
- Full Text
- View/download PDF
53. Characterisation of fatigue and crack propagation in laser shock peened open hole 7075-T73 aluminium specimens
- Author
-
Ivan Meneghin, Vjola Ristori, Goran Ivetic, Enrico Troiani, Andrew M. Venter, Agostino Lanciotti, José Luis Ocaña, C. Polese, Miguel Morales, Gianluca Molinari, Juan Antonio Porro, G. Ivetic, E. Troiani, I. Meneghin, G. Molinari, J.L. Ocaña, M. Morale, J. Porro, A. Lanciotti, V. Ristori, C. Polese, and A. Venter
- Subjects
Laser power density ,Materials science ,Metallurgy ,fungi ,chemistry.chemical_element ,Peening ,Fracture mechanics ,Laser ,FATIGUE ,Shock (mechanics) ,law.invention ,OPEN HOLE ,chemistry ,Residual stress ,Aluminium ,law ,CRACK PROPAGATION ,parasitic diseases ,Composite material ,Open hole ,LASER SHOCK PEENING - Abstract
The goal of this research activity is to evaluate the capability of Laser Shock Peening (LSP) technology to improve fatigue life in open-hole aluminium specimens. Thin, dog-bone specimens were LSP treated in direct ablation mode and subsequently tested. The obtained results have not proven the advantage of LSP technology over traditional residual stress insertion techniques around open-holes, such as cold working. Therefore, the focus of the activity was moved towards understanding the causes of the observed fatigue life reduction.
54. [IgA-IgG-IgM serum levels in blood donors. Examination of some variables]
- Author
-
A, Ghessi, F, Azzario, C, Marinig, L, Mancini, T C, Polese, and V, Pozzessere
- Subjects
Adult ,Male ,Immunodiffusion ,Body Weight ,Age Factors ,Blood Donors ,Middle Aged ,Immunoglobulin A ,Sex Factors ,Immunoglobulin M ,Immunoglobulin G ,Humans ,Female ,Aged - Abstract
IgA, IgG and IgM serum levels in 603 normal blood donors (510 males and 93 females) have been calculated by the method of single radial immunodiffusion. In every immunoglobulin class the normal values and other important statistical parameters have been determined. Several statistical examinations have been executed to test the influence of some factors as sex, weight, age and number of blood donations on IgA, IgG and IgM serum levels.
- Published
- 1976
55. Relationship between residual stresses and welding rates in friction stir welded AA6056-T4
- Author
-
L.A. Cornish, C. Polese, and Oluwaseun John Dada
- Subjects
Materials science ,chemistry.chemical_element ,Welding ,Indentation hardness ,law.invention ,Transverse plane ,chemistry ,law ,Residual stress ,Aluminium ,Ultimate tensile strength ,Friction stir welding ,Composite material ,Strain gauge - Abstract
This paper reports the relationship between residual stress distribution and key friction stir welding parameters, transverse and rotational speeds, on 6056-T4 aeronautical aluminum alloy. Standard electric strain gauge based destructive technique was used to measure residual stresses at the top of the weld in the longitudinal direction. Residual stresses across the weld had the “M” distribution with asymmetry across the advancing and retreating sides. Location of retreating side tensile residual stress maximum was the weakest zone and microhardness minimum across the weld. Tensile longitudinal residual stress minimum and maximum at the retreating side of the weld reduced with increased translational speed and advance per revolution.
56. Professional development and well-being: promoting the practice of statistics in Amstat News
- Author
-
Zollo Sole Alba, Pennarola C., Polese V. and Zollo S.A., and Zollo, SOLE ALBA
- Subjects
Professional well-being, human development, promoting statistics - Abstract
The paper emphasises the need for job satisfaction as a major ingredient of people’s well-being and also suggests that different jobs require different job skills (for example, statisticians resort to creativity and problem-solving). By investigating a sample of Amstat News issues, the American Statistical Association’s monthly magazine, the linguistic analysis has foregrounded the persuasive strategies employed by the organisation to promote the practice and profession of statistics by eradicating some misconstructions about statistics and prioritizing work-life balance and well-being.
- Published
- 2021
57. Telling food and wine stories: storytelling strategies from linguistic and marketing perspectives
- Author
-
D'Avanzo S., Garofano A., D'Avanzo, S., Garofano, A., Pennarola, C., Polese, V., Zollo, S.A., D'Avanzo, S., and Garofano, A.
- Published
- 2021
58. Identifying patterns in Big Data Analytics to enhance value co-creation
- Author
-
KELLER B., MÖHRING M., CARRUBBO L., CAPUTO F., SCHMIDT R., Gummesson E., Mele, C., Polese, F., Keller, B., Möhring, M., Carrubbo, L., Caputo, F., and Schmidt, R.
- Published
- 2021
59. Introduction
- Author
-
Zollo Sole Alba, Sole Alba Zollo, Pennarola C., Polese V. and Zollo S.A., and Zollo, SOLE ALBA
- Subjects
Well-being, human development, cross-disciplinary methodologies - Abstract
This volume brings together different kinds of expertise and disciplinary approaches to human development and well-being, crucial issues in today’s world threatened by such diverse problems as climate change, unequal distribution of wealth and economic exploitation of developing countries, uncontrolled technological progress, systematic violations of human rights, discrimination and racism, health emergencies. The language analysis toolkit ̶ e.g., cross-cultural pragmatics, corpus linguistics, Critical Discourse Analysis, Systemic Functional Linguistics ̶ has been enriched by the analytical tools and frameworks volunteered by our colleagues specialists in demography, economics, international relations, law and political geography. The analysis of the specialized discourses of well-being and human development has meant to investigate to what extent different communities of practice share approaches and methodologies around these current issues.
- Published
- 2021
60. Open Innovation and Industry 4.0: the new frontiers for value co-creation?
- Author
-
Saviano M., Del Giudice M., Pironti M., Caputo F., Gummesson E. Mele C. Polese F., Saviano, M., Del Giudice, M., Pironti, M., and Caputo, F.
- Subjects
Open Innovation ,Value co-creation ,Service perspective ,Industry 4.0 ,Systems perspective - Abstract
Purpose – Innovation and Information and Communications Technologies (ICTs) are new levers on which act for redesigning social and economic relations and structures. The technology shifts produce disruptive effects on business models, having revolutionary impacts on internal growth strategies for the future of manufacturing. Joining the vibrant debate about the role of Innovation and ICTs in ensuring companies’ viable survival, the paper aims at investigating Open Innovation and Industry 4.0 using an interpretative framework rooted in Service and Systems research highlighting new opportunities to advance knowledge about value co-creation. Methodology/approach – A brief literature review on Open Innovation and Industry 4.0 is conducted using the interpretative lens provided by the Service and Systems perspectives. A deductive approach is adopted for interpreting key processes of Open Innovation and Industry 4.0 as drivers for enhancing value co-creation. Findings – The findings direct to identify Technology Readiness, Cognitive Alignment, Collaborative Orientation, and Shared Strong Beliefs as possible drivers that enhance the value co-creation potential created by Open Innovation and Industry 4.0. A framework of synthesis is outlined that can represent a useful reference for exploiting the value co-creation opportunities of Open Innovation and Industry 4.0. Research implications/limitations – The paper contributes to the multi- and inter- disciplinary research stream aimed at overcoming the limitations of a still dominant reductionist view by adopting systems approaches. Analyzing Open Innovation and Industry 4.0 as potential paths for addressing future evolutions in value co-creation studies, the paper enriches previous managerial researches. Nevertheless, the work is currently a preliminary study mainly directed to share ideas and views within the multi-perspective context of the Naples Forum on Service. Practical implications – Valorizing the opportunities for value co-creation linked to Open Innovation and Industry 4.0 practices, the paper provides decision makers with a road map for better understanding and managing critical drivers for effectively implementing value co-creation logics. Originality/value – The paper enriches the ongoing debate about Open Innovation and Industry 4.0 providing new insights through the interpretative lens of the Service and Systems perspectives that highlight the great potential for value co-creation linked to these new technologies.
- Published
- 2019
61. A systems based interpretative framework for approaching exaptation and bricolage in decision making and value co-creation
- Author
-
Barile, S., Saviano, M., DI NAUTA, Primiano, Caputo, F., Lettieri, M., Gummesson E. Mele C. Polese F., Barile, S., Saviano, M., DI NAUTA, Primiano, Caputo, F., and Lettieri, M.
- Subjects
Value co-creation ,Exaptation ,Bricolage ,Systems studies ,Complexity ,Flexibility ,Ecosystems ,Systems studie - Abstract
Purpose – The increasing variety and variability of social and economic dynamics is pushing both researchers and practitioners in developing approaches, models, and tools for supporting decision makers in observing, interpreting, understanding and managing emergent dynamics. For contributing to the ongoing debate, the paper aims at discussing possible paths for facing the challenges of the increasing complexity through flexible decisional and behavioral approaches. Methodology/approach – The paper adopts a qualitative approach for systematizing and refreshing the interpretative role of existing literature within managerial and organizational fields. Through the interpretative contributions provided by the systems thinking, a multi-disciplinarity literature review is conducted for identifying possible constructs through which addressing the development of flexible managerial and organizational models. Findings – Managerial and organizational fields are enriched through the concepts of exaptation and bricolage as explicative domains able to provide useful indications to decision makers interested in building more flexible decisional and behavioral approaches. A possible path for managing the increasing social and economic complexity is depicting in the light of systems perspective, and a system based interpretative framework is defined for explaining actors’ reciprocal adaptation as a way for supporting value co-creation. Research implications – The paper provides possible pillars on which acting to build managerial and organizational approaches able to enhance actors’ contributions through the definition of shared processes. The concepts of exaptation and bricolage enrich previous managerial and organizational literature promoting research on value co-creation as a driver for ensuring systems’ viability in dynamic ecosystems. Practical implications – The paper calls the attention of decision makers on the need for the systems’ flexibility trough the enhancement of actors’ contributions for facing the challenges of increasing social and economic complexity. Acting on exaptation and bricolage, a possible model is provided for clarifying managerial and organizational paths for increasing the systems’ flexibility and for supporting practitioners in defining approaches able to support actors’ collaboration as a required condition for value co-creation. Originality/value – Adopting a multi-disciplinary perspective the paper enriches previous managerial and organizational literature trough the definition of concepts, processes, and approaches able to support both researchers and practitioners for better understanding and managing increasing social and economic complexity by acting on exaptation and bricolage. Thanks to the interpretative lens provided by the systems studies, a conceptual model for addressing systems’ decisions and behaviors in the light of value co-creation is proposed.
- Published
- 2019
62. Changing role of suppliers-customers and smart technologies: a systematic review on energy management
- Author
-
Amitrano Cristina Caterina, Bifulco Francesco, Gummesson E., Mele C., Polese F., Amitrano, CRISTINA CATERINA, and Bifulco, Francesco
- Subjects
energy management ,prosumer ,Smart technologie ,S-D logic - Abstract
The aim of this research is to adopt the Service-Dominant (S-D) logic lens (Vargo et al., 2008; Akaka & Vargo, 2014) to analyse a particular service industry, namely energy, in order to identify the ongoing and future trajectories. The main empirical contexts analysed in service scholars’ studies are usually retail (Willems et al., 2016; Balaji & Roy, 2017), healthcare (McColl- Kennedy et al., 2017), and tourism (Carlson et al., 2016) while energy is mainly studied by engineers with research published in non-service journals. However, energy management is a field of study strictly related to the changing role of suppliers and customers where the concept of prosumer (Chandler & Chen, 2015) is widely adopted (Espe et al., 2018; Zafar et al., 2018). So, the S-D logic can be very useful in order to better understand this phenomenon, stimulating service scholars’ attention to energy management as a particular context of analysis for empirical research.
- Published
- 2019
63. An omnichannel approach for value propositions
- Author
-
GARGIULO, ROBERTA, Bifulco Francesco, Gummesson E., Mele C., Polese F., Gargiulo, Roberta, and Bifulco, Francesco
- Subjects
Omnichannel, value, engagement, S-D logic, cultural heritage - Abstract
Service marketing literature suggests that omnichannel approach (Verhoef et al., 2015) can influence engagement metrics such as, among others, awareness, conversions, cross-channel, customer retention, customer lifetime value (Ailawadi and Farris, 2017; Broussard 2016) and can generate a positive word-of-mouth (advocacy) and a higher revisit rate (Sopadjieva et al., 2017). Starting from the point that “a customer is always a co-creator of value” (Vargo and Lusch 2006), firms are trying to combine physical and digital touch points (Verhoef et al., 2015) with the aim of providing the customer a seamless experience with no barriers between channels (Rodríguez-Torrico et al., 2017 Ailawadi and Farris, 2017; Lemon and Verhoef, 2016; Beck and Rygl, 2015) which lead to greater engagement and value co-creation (Payne et al., 2017; Nordgren and Johansson, 2017). The aim of this work is to analyse how the omnichannel strategy, as a communication proposition, can influence customers’ value through the lens of S-D Logic.
- Published
- 2019
64. Combining the pillars of the Naples forum
- Author
-
Caputo, Francesco, Walletzky, Leonard, Mouzhi, Ge, Carrubbo, Luca, Gummesson E. Mele C. Polese F., Caputo, Francesco, Walletzky, Leonard, Mouzhi, Ge, and Carrubbo, Luca
- Subjects
Service Dominant Logic ,Service Science ,Network and Systems Theory ,Recommender system ,IBM Bluemix - Abstract
Purpose – The paper aims to enrich conceptual frameworks of Service Dominant Logic, Service Science, and Network and Systems Theory by integrating their pillars in a common interpretative approach and providing a tool to support a better understanding of service functioning as well as design in Complex Adaptive Systems (CAS). Design/Methodology/approach – The paper proposes an integrated literature review in order to link Service Dominant Logic, Service Science, and Network and Systems Theory in a common trans-disciplinary conceptual framework. Building upon this, the practical approach of recommender system and the modelling functionalities of IBM Bluemix are used to define an application overview of service design in CAS. Finally, theoretical and empirical reflections herein are donated to the context of Smart City as an example of CAS. Findings – The paper proposes a multi-dimensional constructive tool for service and systems analysis in order to better understand the interactions among users, services, and infrastructures in a shared environment. Research limitations/implications – Although it is challenging to bring the Service Dominant Logic, Service Science, and Network and Systems Theory together, we have initially taken a theoretical lens to view the previous studies from Service Dominant Logic, Service Science, and Network and Systems Theory. This intends to connect their fundamental pillars in order to support a wider understanding of their dimensions and dynamics in CAS. Practical implications – We have deployed the proposed framework in IBM Bluemix, which is a real-world shared environment. This finding outlines the effective interactions between users, services, and infrastructures, which indicate that more alternatives and outcomes can be obtained by designing service and systems interactions. Therefore, for researchers and practitioners, this paper implicates that designing the services and related interactions is critical to CAS and it is important to focus on the design of services in Smart City. Originality/value – The paper has contributed to the state of knowledge in the domains of Service Dominant Logic, Service Science, as well as Network and Systems Theory by providing an effective application of a shared conceptual framework. Both theoretical and practical contributions can be used to build an effective application of multi- and trans- disciplinary models in order to manage networks and service systems.
- Published
- 2017
65. T-Shaped People for addressing the Global Challenge of Sustainability
- Author
-
Barile, Sergio, Saviano, Marialuisa, Polese, Francesco, Caputo, Francesco, Gummesson E. Mele C. Polese F., Barile, Sergio, Saviano, Marialuisa, Polese, Francesco, and Caputo, Francesco
- Subjects
Viable Systems Approach ,T-shaped Professionals, Sustainability Science ,Education for Sustainable Development ,Systems thinking ,T-shaped Professionals ,Sustainability Science ,T-shaped Professional - Abstract
Purpose – Poverty, hunger, inequalities, diseases, unsustainable use of resources, etc., in spite of advances of the last decades, still result unsolved worldwide issues. All relate to the challenges of sustainability and sustainable development that now call for urgent answers. Education is one of the key drivers of a really transformative change. To address this change, however, a profound re-thinking of education programs is required, as has emerged at the end of the UNESCO’s Decade of Education for Sustainable Development. The purpose of this paper is to explore how the scientific community of the Naples Forum on Service responds to this call. Design/Methodology/approach – By adopting a “3Pillars-Based” integrated perspective, key assumptions and findings from the three scientific communities of the Forum are identified to find foundational elements of a lifelong education process, targeted to address the multiple challenges of sustainability and sustainable development. Findings – Through the interpretative lens of the Viable Systems Approach, convergence between the three scientific proposals of Network and Systems Theory, Service-Dominant logic and Service Science, relevant to the building of a common framework for re-thinking education, are discussed. Findings indicate the skills with which decision makers must be endowed to face the challenges of transformative change toward sustainability and sustainable development. Boundary crossing and systems thinking capabilities are specifically identified as key skills to be developed. These findings suggest the opportunity to consider the “T-Shape” model as a general reference for re-thinking education methodologies and programs, as it implies an effective integration of soft and hard skills. A trans-disciplinary systems thinking based body of theoretical and practical knowledge is required as fostered by Sustainability Science. Education of T-shaped People may be a solution. Research implications - An integrated effort of scientific communities engaged in research that can contribute to the global call for a more sustainable and inclusive world, is essential. Practical implications – Trans-disciplinarity implies the involvement of people from the business and social real world in the education process, to test and put in practice advances by adopting real problem solving approach. Originality/value – This paper represents a call for engaging the Naples Forum on Service scientific and professional communities in worldwide collaboration to contribute to address the global challenge of a more sustainable and inclusive world by leveraging on education. Paper type – Conceptual paper
- Published
- 2015
66. Consumer Brand Engagement Positioning in Marketing Theory under a Service Dominant Logic Perspective
- Author
-
CANTONE, LUIGI, TESTA, PIERPAOLO, Marrone T., Gummesson, E., Mele, C., Polese, F., Cantone, Luigi, Testa, Pierpaolo, and Marrone, T.
- Subjects
Consumer Brand Engagement, Customer Engagement, SDL - Abstract
The paper is a theoretical contribution on the role of consumer brand engagement (CBE) in marketing theory and moves within the Service Dominant Logic paradigm. The paper aims to identify the distinctive positioning of consumer brand engagement topic in marketing literature filling a literature gap and contributing to a better definition of the concept useful also for a good managerial practice. Several Authors have investigated the interface between CBE and SDL (Merz and Vargo, 2009; Hollebeek 2013, Brodie, et al. 2006). However, still remain in marketing literature a certain confusion and overlapping among several research topics that probably derive from an unclear systematization of brand theory in particular that related to brand feelings (brand love, brand attachment, brand engagement) and consumer active role in Brand Equity Creation. The literature contribution on CBE suffer this limitations. As a consequence of that, it is very difficult to identify an effective scale measurement that measures CBE and not something else (brand experience, brand image, brand perceptions, consumer brand resistance, brand antagonism, and so on). This probably because CBE topic is still under-conceptualized and, consequently, under-investigated. Methodology/Research design/Approach for empirical investigation. The methodology of empirical investigation is qualitative (Gummesson, 2005; Cantone and Testa, 2011) and quantitative based on Multiple Components Analysis (MCA) protocol (Stewart, 1981)
- Published
- 2015
67. Value in use and in (social) context. Analysing how social actors co-create value
- Author
-
Stampacchia, Paolo, Edvardsson, Robert, COLURCIO, MARIA, COPPOLA, Mariarosaria, Gummesson, E., Mele, C., Polese, F., Stampacchia, Paolo, Colurcio, Maria, Edvardsson, Robert, and Coppola, Mariarosaria
- Published
- 2015
68. Innovation in multiple contexts: searching in the jungle of innovation conceptualisations
- Author
-
Tregua M., Russo Spena T., Bifulco F., Gummesson E., Mele C., Polese F., Tregua, M., Russo Spena, T., and Bifulco, F.
- Published
- 2015
69. The three pillars of the ‘the 2013 Naples Forum on Service’
- Author
-
Gummesson E., Polese F., MELE, CRISTINA, Gummesson E., Mele C., Polese F., Gummesson, E., Mele, Cristina, and Polese, F.
- Published
- 2013
70. Co-innovating: the practices of collaborative innovation
- Author
-
MELE, CRISTINA, RUSSO SPENA, TIZIANA, Gummesson E., Mele C., Polese F., Mele, Cristina, and RUSSO SPENA, Tiziana
- Published
- 2013
71. Understanding market plasticity: the dialectic dynamics between stability and fluidity
- Author
-
Nenonen S., Cheung L., Kjellberg H., Lindeman S., Pels J., Sajtos L., Storbacka K., MELE, CRISTINA, Gummesson E., Mele C., Polese F., Nenonen, S., Cheung, L., Kjellberg, H., Lindeman, S., Mele, Cristina, Pels, J., Sajtos, L., and Storbacka, K.
- Published
- 2013
72. Towards a value co-creation based Healthcare System
- Author
-
Calabrese, Mario, Iandolo, Francesca, Antonucci, Emanuela, Caputo, Francesco, Gummesson E. Mele C. Polese F., Calabrese, Mario, Iandolo, Francesca, Antonucci, Emanuela, and Caputo, Francesco
- Subjects
Viable Systems Approach ,Public-private report ,Value co-creation ,Healthcare System ,Information Variety Model ,Public-private reports - Published
- 2013
73. Integrating the three pillars: S-D Logic, Network & Systems theory and Service Science: Integrating the perspectives for a new research agenda
- Author
-
MELE, CRISTINA, Gummesson E., Mele C., Polese F., and Mele, Cristina
- Published
- 2011
74. Productions of excellence as territory attraction’s factors
- Author
-
DELLA CORTE, VALENTINA, SAVASTANO, IRIS, Gummesson E., Mele C., Polese F., DELLA CORTE, Valentina, and Savastano, Iris
- Subjects
"Luxury" ,"attractive factors" ,"networking" - Abstract
The paper evaluates the potential development of a territory’s productions of excellence, which have to express territory’s identity and have to represent a call for luxury’s tourism. Enterprises of luxury’s products seem not to be affected by the global crisis as they keep having niche consumers and they invest in quality and innovation. The research intends: to analyze the concept of luxury from a request and offer’s point of view; to verify the operative proposals to realize specific luxury tourism chain; to evaluate this kind of productions as attractive factors to increase territory tourism’s flows in the areas where companies are placed, considering consumers as co-producers. The draft starts from the literature of the luxury’s concept, considered either in the S-D Logic (Vargo, Della Corte, Mele) and in the RBT. This method brings to a complex overview of a more difficult value creation process, produced by the link of several actors who cooperate to have a systemic offer of a territory productions of excellence. The potential researches intend to verify: hp1) Which is the running and perspective role of the luxury customer? hp2) which is the luxury production’s configuration? hp3) are there systemic features to evaluate/enforce territorial productions of excellence? The empiric research has been made through the analysis of the main production field of excellence in Naples area, elaborating several companies examples and territory study.
- Published
- 2011
75. Quality in Marketing Process and a Systemic Value Co-creation
- Author
-
DELLA CORTE, VALENTINA, DI TARANTO, ENRICO, MICERA, ROBERTO, Gummesson E., Mele C., Polese F., DELLA CORTE, Valentina, DI TARANTO, Enrico, and Micera, Roberto
- Subjects
quality ,network ,resource ,value co-creation ,destination image - Abstract
The purpose at the basis of this research is to demonstrate that the network strategy influences the complex tourist product quality and if the firms pursue a co-planning value strategy, this latter affects marketing choices of tourist product.We propose a theoretical model which represents an application of service-dominant logic according to resource-based theory, with specific attention to a particular branch and precisely how the “relational view” leads to value co-creation and that takes into account the central issue of tourism and in particular hotel quality management. We demonstrate the usefulness of this framework through a structured questionnaire, submitted to a sample of hotels, helpful to understand the club’s implications on the quality co-creation process. According to the high quality hotels’ offering, Campania’s hotels have to improve network relationships with local authorities and private firms, as well as regional offer forms, creating adequate conditions of competitiveness on global tourist market.
- Published
- 2011
76. Co's in innovating: co-creation within a practice based view
- Author
-
MELE, CRISTINA, RUSSO SPENA, TIZIANA, Gummesson E., Mele C., Polese F., Mele, Cristina, and RUSSO SPENA, Tiziana
- Abstract
Purpose Despite many studies about co-creation in innovation, a comprehensive understanding of all the elements that shape the process of co-creating innovation is still lacking. We aim to begin filling this gap. This paper is designed to frame innovation as a process of co-creation according to a practice-based view. Methodology/approach First, we draw from the work of Frow Payne and Storbacka (2010) and Frow, Brodie, Little, Payne (2010) and conceptualise co-creation as a series of “co.” Second, we adopt a practice-based perspective and conceptualise innovation as a collection of practices. We outline two research propositions which guide the empirical research carried out on ten companies. We focus our analysis on the encounter process as it involves practices of interaction. Moreover, we analysed a specific encounter contest, “the web sites”. Findings Companies developed specific web sites to foster innovation by a network of actors. This practice is a new way to innovate and extends through the Internet. We present the findings according to two dimensions:1 ) the “co‟s” for innovation and 2) the elements of practices. The “co‟s” for innovation can be seen as different phases of innovation processes in which actors interact, collaborate and integrate resources. We identified the following “co‟s”: co-generation of ideas, co-evaluation of ideas, co-design and co-launch. Within each “co,” we identified practices and elements of practices, namely actors, actions, tools, and images. Practical Implications This work addressed the need to frame innovation in terms of a group of interactions within a constellation of practices. From this perspective, co-creation in innovation shifts its focus from customer collaboration to the creation of new value proposals by several actors with the focal firm‟s engagement in the creation of practices supporting other actors‟ value-creating processes. Originality/value In finding a fresh conceptualization of innovation, the authors move from the outcome to the process, that is, from innovation (as a new artefact) to innovating (as a set of co-creation practices). This works can stimulate the debate about innovation and co-creation.
- Published
- 2011
77. A Second Chance at Life? Analyzing Customer Value in the Medical Industry
- Author
-
AGUIARI, Roberto, PALADINO A., Gummesson E., Mele C., Polese F., Aguiari, Roberto, and Paladino, A.
- Subjects
market orientation, innovation performance, financial performance, medical innovations, resource-based view, resource orientation - Abstract
Purpose This case reviews how a specialist medical organization in Europe has been able to build sustained positive market value by simultaneously focusing on a market orientation as well as a resource orientation. Design/methodology/approach The case study draws on market orientation, the resource-based view and customer value theories and uses interviews, internal company documentation and secondary research. Findings The case provides an analysis of how an organization has adopted elements of market and customer strategies to survive in an intensely competitive knowledge-driven industry. Findings show that effective differentiation for medical equipment is required to increase client adoption levels and the likelihood of purchase (by medical specialists and patients). From the case, it is apparent that the success of specialist medical devices depends on attention to customer value, as well as product and service quality. These are each driven by facets of resource and market orientations. Originality/value This study provides support for emerging research which demonstrates that a dual focus on the resource composition of an organization, as well as the focus on the provision of customer value are able to drive innovation and financial performance. It shows that organizations cannot limit their focus on one strategic orientation to be successful.
- Published
- 2010
78. Co-create value innovation as a resource integration process
- Author
-
MELE, CRISTINA, RUSSO SPENA, TIZIANA, COLURCIO M., Gummesson E., Mele C., Polese F., Mele, Cristina, RUSSO SPENA, Tiziana, and Colurcio, M.
- Subjects
resource integration ,network ,Innovation - Abstract
Purpose. S-D logic and service science are reframing the conceptual landscape of business. From these perspectives, performing business means for firms to act as resource facilitators and integrators, connecting service systems via value proposals. The aim of the paper is to analyse innovation as a value-creating process occurring through many-to-many resource integration. The article will argue for a merging of innovation, S-D logic, and network approaches. Methodology. A case study has been carried out. First, we selected a highly innovative firm and focused on conception of and approach to innovations. In detail, we investigated six innovation projects within three main research streams. Then we identified the innovation projects’ networks. The problem of network boundaries (who and where) was handled by focusing the study on the main actors involved in the innovation projects. By studying the interaction and integration process that takes place between the network’s partners, we have been able to highlight innovative patterns, resource contribution to innovation, and the value co-creation process. Findings. Innovation is a network affair, carried out through interaction and with an integration process in which several actors participate as advocated S-D logic. This changes the traditional vision of innovation, in which the supplier is the innovator and the customer a user or source of innovation. Customers and other stakeholders become the real co-innovators, and the innovation process is developed by continuous interactions among stakeholders through which they develop, exchange and integrate resources and co-create value. Partners and networks are seen as a set of potential resources that are to be matched through new value proposals and that need actualisation to create new value in use. Practical implications. A company or a service system should develop the innovation process, managing the contribution of the network’s members and considering both B2B and B2C/C2B (as well as C2C) interactions part of an integrated, complex context (many-to-many). Innovation development should be framed as an open process in which all of the network’s actors can mobilise resources and be co-innovators, co-producing value innovation as a step toward creating value for themselves and for the others. To develop open innovation, managers should draw on the potential of resources, competencies, and talent—not only inside the firm, but above all, from the residents of its ecosystem. Research implications. There is a clear need to develop further researches where innovation is framed as a resource-integrating process. Studies could widen the unit of analysis from firm to network and from dyadic to multiple actors, and enlighten us further as to the antecedents and implications of the interaction and integration process. Originality/Value Framing innovation with S-D logic allows companies and other service systems to move the locus of innovation from product to value, supporting service flow and transforming their understanding of value from a vision based on units of the firm’s output to one based on processes that integrate resources. Innovation is a key theme in service science. This paper offers a wide perspective on innovation to frame the phenomenon using a service-dominant logic, highlighting the role of the network in developing value-creating innovation (processes and results) as instrumental to increasing stakeholder value.
- Published
- 2009
79. Alternative Logics for Innovation: a call for service innovation research
- Author
-
MELE, CRISTINA, RUSSO SPENA, TIZIANA, COLURCIO M., Gummesson E., Mele C., Polese F., Mele, Cristina, Colurcio, M., and RUSSO SPENA, Tiziana
- Subjects
iNNOVATION ,network ,service - Abstract
The aim of this paper is to frame innovation within S-D Logic and Service Science and propose a framework in order to launch a call for service innovation research. Methodology. Through a review of the literature, we analyse innovation using different approaches such as goods-dominant logic, S-D logic, and cognitive-relational studies as a transitional view. Findings. We outline the main elements of each approach and develop a framework with a focus on their differences in terms of drivers, outcomes, processes, and actors‘ roles. Innovation within goods-dominant logic is analysed in terms of new product development and new service development. In both of these research streams, innovation is seen as an output (a new good or a new service), coming out from an organisational internal process where the firm is the main actor, protecting its knowledge with an owner and secret approach. The cognitive-relational approach provides a different perspective on innovation in which the drivers of the process are knowledge, competencies, and relationships. The firm is still the main innovator, with the key users and partners acting as sources of knowledge that is used to produce superior value for the recipients. Recognising the role of resource-based view, S-D logic moves the focus to value-creating innovation. This is an ‗open‘ innovation process in which all actors in the network can mobilise their resources to become co-innovators and co-producers of value. Research implications Our theoretical findings represent a good basis upon which further studies of innovation can be undertaken. We call for the development of models and innovation patterns within S-D logic and Service Science. Originality/Value Innovation is a key theme in service systems and service science. However, studies are widely based on a goods-dominant logic, even when the offering is itself a service. This paper offers a new and wider perspective on innovation to frame the phenomenon in S-D logic as a basis for further studies.
- Published
- 2009
80. Service Science, S-D Logic and network theory: Integratig the perspectives for a new research agenda
- Author
-
GUMMESSON E., POLESE F., MELE, CRISTINA, Gummesson E., Mele C., Polese F., Gummesson, E., Mele, Cristina, and Polese, F.
- Subjects
Service ,Network ,system - Published
- 2009
81. Performance and bone characteristics of broilers fed diets supplemented with vitamin A at different concentrations.
- Author
-
Savaris VDL, Pozza PC, Polese C, de Vargas JG, Pavlak MSD, Wachholz L, Vieira BS, Tesser GLS, de Oliveira Carvalho PL, Eyng C, and Nunes RV
- Subjects
- Animals, Animal Feed analysis, Animal Nutritional Physiological Phenomena, Diet veterinary, Dietary Supplements analysis, Nutritional Status, Chickens physiology, Vitamin A pharmacology
- Abstract
This work evaluated the influence of vitamin A on performance, organ weight, and bone and skin characteristics in broilers (Cobb 500) at 21 and 42 days of age. A total of 1920 chickens were distributed in a randomised design, considering six vitamin A supplementation levels (0, 6000, 16,000, 26,000, 36,000, and 46,000 IU kg
-1 ), with 16 replicates and 20 chickens per experimental unit, established due to rising the range of vitamin levels observed in the literature to evaluate the effect of vitamin A on broilers. At 22 days, half of the replicates from each treatment continued receiving the initial diet, and the other eight repetitions received diets without vitamin A (0 IU kg-1 ) until 42 days. The level of vitamin A influenced feed intake (FI) and body weight gain (BWG) until 21 days for all treatments. Broilers at 21 days of age had a more significant BWG at a vitamin A supplementation level of 28,209 IU kg-1 . At 42 days, vitamin A influenced the BWG and FI of broilers at treatments that were not supplemented after 21 days. Treatments supplemented up to 42 days showed quadratic responses to vitamin A for BWG, FI, and feed conversion. The vitamin A levels influenced the relative weights of the small intestine, pancreas, gizzard, abdominal fat, Seedor index, and breaking strength at 42 days, where the adequate supplementation of vitamin A improved these characteristics in broilers. Vitamin A supplementation from 22 to 42 days old did not affect broiler performance. An increased BWG was obtained when vitamin A supplementation occurred until 42 days, with supplementation of 29,375 IU kg-1 and a lower response of feed conversion with the addition of 27,775 IU kg-1 ., (© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.)- Published
- 2024
- Full Text
- View/download PDF
82. Zeolite and corn with different compositions in broiler chickens feeding.
- Author
-
Pavlak MSD, Kaufmann C, Eyng C, Carvalho PLO, Pozza PC, Vieites FM, Rohloff Junior N, Avila AS, Polese C, and Nunes RV
- Subjects
- Animals, Male, Zea mays, Animal Nutritional Physiological Phenomena, Diet veterinary, Animal Feed analysis, Dietary Supplements, Chickens, Zeolites
- Abstract
The objective of this work was to evaluate the behavior of zeolite against different types of corn in broiler chickens' diets. 1,200 male broiler chickens were assigned to 6 diets in a completely randomized design and a 2 × 3 factorial arrangement, consisting of 2 types of corn (higher or lower mycotoxin contamination) and the inclusion of zeolite (0; 5,000 and 10,000 g ton
-1 ). In the period from d 1 to 21, there was an interaction (P = 0.0040) between types of corn and the inclusion of zeolite for feed conversion ratio (FCR). In the phase from 1 to 42 d, there was an interaction (P = 0.0322) on the serum levels of creatinine (CREA) and digestible gross energy (dGE); corn with lower mycotoxin level contamination (LMLC), caused a reduction in body weight gain (BWG) (P = 0.0046) and increase in the relative weight of abdominal fat (P = 0.0256). Inclusion rates of zeolite promoted an increase in the digestible CP (P = 0.0477) and digestible ash (P < 0.0001), as well as an increase (P < 0.0001) in hot carcass yield (P = 0.0433). The results indicate that the inclusion of zeolite, in the amounts used, did not alter the performance, serum levels, intestinal development and litter quality of the birds at 42 d of age. However, it was responsible for the improvement in the percentage of digestible nutrients (CP, GE, and MM)., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
83. Performance, metabolism, and meat quality of broilers fed dry brewery residue.
- Author
-
Tesser GLS, de Avila AS, Broch J, de Souza C, Polese C, Kaufmann C, Eyng C, Savaris VDL, Junior NR, Bruno LDG, Cella PS, and Nunes RV
- Subjects
- Animals, Male, Albumins, Eating, Medroxyprogesterone Acetate, Chickens, Meat
- Abstract
A study was conducted to assess the chemical composition and apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and their respective coefficients of dry brewery residue (DBR) for broilers. A second study was conducted to evaluate the increasing inclusions of DBR in broiler diets and its effects on growth performance, carcass traits, blood metabolites, and meat quality. In the first trial (metabolism assay), a total of 250 male broilers were assigned to 5 diets (0, 100, 200, 300, and 400 g DBR/kg) composed by 5 replications with 10 animals per cage in a completely randomized design in the period from days 11 to 21. There was an increasing linear effect for AME, AMEn, metabolizable coefficient of gross energy (MCGE), and MCGE corrected for N (MCGEn). In the second experiment, a total of 840 one-day-old male broiler chickens were assigned to 6 treatments (0, 20, 40, 60, 80, and 100 g DBR/kg) with 7 replications and 20 animals per pen, from days 0 to 42. Growth performance was determined at days 21 and 42. There was an increasing linear effect for weight gain in the period from days 0 to 21 and for feed intake from days 0 to 42. A linear decreasing effect was observed at day 21 for cholesterol and quadratic effect for uric acid (UA), aspartate aminotransferase, and albumin. At day 42, there was a quadratic effect for UA and creatinine. At day 21, an increasing linear effect was observed for protein deposition rate. In conclusion, the inclusion of DBR up to 100 g/kg improved WG in the starter phase and did not negatively affect the productive parameters of broilers from days 0 to 42., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
- Published
- 2022
- Full Text
- View/download PDF
84. Effects of vitamin A on carcass and meat quality of broilers.
- Author
-
Savaris VDL, Broch J, de Souza C, Rohloff Junior N, de Avila AS, Polese C, Kaufmann C, de Oliveira Carvalho PL, Pozza PC, Vieites FM, and Nunes RV
- Subjects
- Animal Feed analysis, Animals, Diet veterinary, Meat analysis, Pectoralis Muscles, Chickens, Vitamin A
- Abstract
This study evaluated the level and length of time of vitamin A supplementation and its effects on carcass and cuts yield, meat quality, and myopathies in 42-day-old broilers. A total of 1,920 birds were divided into 6 groups, and each group received a different level of vitamin A: 0; 6,000; 16,000; 26,000; 36,000 and 46,000 IU/ kg. From d 1 to 21, the treatments were distributed among 16 replicates with 20 birds. From the 22nd d on, 8 repetitions remained with the initial treatment and the others received diets with no vitamin A supplementation. Twelve birds were slaughtered per treatment to evaluate carcass and cuts yield, shear force, cooking loss, water holding capacity, and the presence of substances reactive to thiobarbituric acid. The remaining birds were slaughtered and evaluated in loco for Wooden Breast (WB) and White Striping (WS). Wings weight was affected by vitamin A levels. The duration of the vitamin A supplementation process had effects on the weight of breast, legs with a dorsal portion, and meat color in the yellow intensity (b*). Incidence of WB had higher scores in birds supplemented until 42 d of age. WS showed a quadratic response and a lower response with supplementation of 29,700 IU/ kg. Even for WS, a higher occurrence of the normal score was found in birds supplemented until 21 d of age. Minimal quadratic responses were obtained for normal, moderate, and severe scores, in supplementations of 29,301; 29,959, and 29,827 IU/ kg, respectively. WB had lower occurrence rates in birds supplemented until 21 d of age. Consequently, the severe score was more frequent when supplementation was provided until 42 d of age. The level of vitamin A and the length of time during which this supplementation was provided had influence on cuts yield, meat color and the incidence of WB and WS of the 42-day-old birds., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
85. Interactions between lipid source and vitamin A on broiler performance, blood parameters, fat and protein deposition rate, and bone development.
- Author
-
Savaris VDL, Souza C, Wachholz L, Broch J, Polese C, Carvalho PLO, Pozza PC, Eyng C, and Nunes RV
- Subjects
- Animals, Chickens blood, Chickens growth & development, Chickens metabolism, Diet veterinary, Male, Random Allocation, Vitamins pharmacology, Animal Feed analysis, Animal Nutritional Physiological Phenomena, Bone Development drug effects, Dietary Fats, Unsaturated pharmacology, Dietary Supplements, Vitamin A pharmacology
- Abstract
A total of 2622 male broilers were distributed in a 2 × 5 factorial design, using 2 lipid sources (soybean oil and palm fat), 5 levels of vitamin A supplementation (0, 3,000, 6,000, 12,000, and 24,000 IU kg
-1 ), with 10 replicates, and also 1 control diet (CD) for each lipid source used (7 replicates), each experimental unit being composed of 23 birds. During the first 21 d (how were the birds fed) and from 22 to 42 d of age, a redistribution of the treatments was carried out in a 2 × 2 × 5 factorial design: half of the repetitions of each treatment received the diet of the initial treatment, and the others received the CD with its type of lipid source. In the phase from 1 to 21 d of age, the effect of lipid source on feed intake (FI) and feed conversion ratio (FCR), and the effect of vitamin supplementation on FI and weight gain (WG) were observed, with a quadratic response for both variables. At 42 d of age, the lipid source and vitamin A level influenced the FI, whereas the WG and FCR showed interactions between period and the level of vitamin A supplementation. Neither lipid source resulted in blood parameters out of the typical pattern for birds, and the same was observed in relation to dietary vitamin A supplementation. From 1 to 21 d of age, a vitamin A supplementation of 15,585 IU kg-1 was estimated, and at 42 d, 15,527 IU kg-1 and 15,148 IU kg-1 were estimated for the periods 1 to 21 d and 1 to 42 d, respectively., (Copyright © 2020. Published by Elsevier Inc.)- Published
- 2021
- Full Text
- View/download PDF
86. Metabolic inhibitors accentuate the anti-tumoral effect of HDAC5 inhibition.
- Author
-
Hendrick E, Peixoto P, Blomme A, Polese C, Matheus N, Cimino J, Frère A, Mouithys-Mickalad A, Serteyn D, Bettendorff L, Elmoualij B, De Tullio P, Eppe G, Dequiedt F, Castronovo V, and Mottet D
- Subjects
- Apoptosis drug effects, Cell Line, Tumor, Glucose metabolism, Glutamine metabolism, Humans, Mitochondria drug effects, Mitochondria metabolism, Oxidative Stress drug effects, Reactive Oxygen Species metabolism, Antineoplastic Agents pharmacology, Histone Deacetylase Inhibitors pharmacology, Histone Deacetylases metabolism
- Abstract
The US FDA approval of broad-spectrum histone deacetylase (HDAC) inhibitors has firmly laid the cancer community to explore HDAC inhibition as a therapeutic approach for cancer treatment. Hitting one HDAC member could yield clinical benefit but this required a complete understanding of the functions of the different HDAC members. Here we explored the consequences of specific HDAC5 inhibition in cancer cells. We demonstrated that HDAC5 inhibition induces an iron-dependent reactive oxygen species (ROS) production, ultimately leading to apoptotic cell death as well as mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). Interestingly, adaptation of HDAC5-depleted cells to oxidative stress passes through reprogramming of metabolic pathways towards glucose and glutamine. Therefore, interference with both glucose and glutamine supply in HDAC5-inhibited cancer cells significantly increases apoptotic cell death and reduces tumour growth in vivo; providing insight into a valuable clinical strategy combining the selective inhibition of HDAC5 with various inhibitors of metabolism as a new therapy to kill cancer cells.
- Published
- 2017
- Full Text
- View/download PDF
87. Zinc triggers a complex transcriptional and post-transcriptional regulation of the metal homeostasis gene FRD3 in Arabidopsis relatives.
- Author
-
Charlier JB, Polese C, Nouet C, Carnol M, Bosman B, Krämer U, Motte P, and Hanikenne M
- Subjects
- 5' Untranslated Regions genetics, Arabidopsis drug effects, Arabidopsis Proteins metabolism, Gene Expression Profiling, Genotype, Homeostasis drug effects, Membrane Transport Proteins metabolism, Protein Biosynthesis drug effects, RNA Stability drug effects, RNA Stability genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Transcription Initiation Site, Arabidopsis genetics, Arabidopsis Proteins genetics, Gene Expression Regulation, Plant drug effects, Genes, Plant, Homeostasis genetics, Membrane Transport Proteins genetics, Transcription, Genetic drug effects, Zinc pharmacology
- Abstract
In Arabidopsis thaliana, FRD3 (FERRIC CHELATE REDUCTASE DEFECTIVE 3) plays a central role in metal homeostasis. FRD3 is among a set of metal homeostasis genes that are constitutively highly expressed in roots and shoots of Arabidopsis halleri, a zinc hyperaccumulating and hypertolerant species. Here, we examined the regulation of FRD3 by zinc in both species to shed light on the evolutionary processes underlying the evolution of hyperaccumulation in A. halleri. We combined gene expression studies with the use of β-glucuronidase and green fluorescent protein reporter constructs to compare the expression profile and transcriptional and post-transcriptional regulation of FRD3 in both species. The AtFRD3 and AhFRD3 genes displayed a conserved expression profile. In A. thaliana, alternative transcription initiation sites from two promoters determined transcript variants that were differentially regulated by zinc supply in roots and shoots to favour the most highly translated variant under zinc-excess conditions. In A. halleri, a single transcript variant with higher transcript stability and enhanced translation has been maintained. The FRD3 gene thus undergoes complex transcriptional and post-transcriptional regulation in Arabidopsis relatives. Our study reveals that a diverse set of mechanisms underlie increased gene dosage in the A. halleri lineage and illustrates how an environmental challenge can alter gene regulation., (© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Published
- 2015
- Full Text
- View/download PDF
88. [HDAC5 inhibition: a tool to stop cancer cell immortality].
- Author
-
Polese C and Mottet D
- Subjects
- Cell Survival drug effects, Chromatin drug effects, Chromatin metabolism, Histone Deacetylases genetics, Humans, Neoplasms pathology, Telomere Homeostasis drug effects, Telomere Homeostasis genetics, Apoptosis drug effects, Histone Deacetylase Inhibitors therapeutic use, Histone Deacetylases metabolism, Neoplasms drug therapy
- Published
- 2014
- Full Text
- View/download PDF
89. A new role for histone deacetylase 5 in the maintenance of long telomeres.
- Author
-
Novo CL, Polese C, Matheus N, Decottignies A, Londono-Vallejo A, Castronovo V, and Mottet D
- Subjects
- Apoptosis genetics, Blotting, Western, Electrophoresis, Gel, Two-Dimensional, Fluorescent Antibody Technique, Histone Deacetylases genetics, Human Umbilical Vein Endothelial Cells enzymology, Humans, In Situ Hybridization, Fluorescence, Recombination, Genetic genetics, Telomere genetics, Apoptosis physiology, Histone Deacetylases metabolism, Human Umbilical Vein Endothelial Cells metabolism, Telomere metabolism
- Abstract
Telomeres are major regulators of genome stability and cell proliferation. A detailed understanding of the mechanisms involved in their maintenance is of foremost importance. Of those, telomere chromatin remodeling is probably the least studied; thus, we intended to explore the role of a specific histone deacetylase on telomere maintenance. We uncovered a new role for histone deacetylase 5 (HDAC5) in telomere biology. We report that HDAC5 is recruited to the long telomeres of osteosarcoma- and fibrosarcoma-derived cell lines, where it ensures proper maintenance of these repetitive regions. Indeed, depletion of HDAC5 by RNAi resulted in the shortening of longer telomeres and homogenization of telomere length in cells that use either telomerase or an alternative mechanism of telomere maintenance. Furthermore, we present evidence for the activation of telomere recombination on depletion of HDAC5 in fibrosarcoma telomerase-positive cancer cells. Of potential importance, we also found that depletion of HDAC5 sensitizes cancer cells with long telomeres to chemotherapeutic drugs. Cells with shorter telomeres were used to control the specificity of HDAC5 role in the maintenance of long telomeres. HDAC5 is essential for the length maintenance of long telomeres and its depletion is required for sensitization of cancer cells with long telomeres to chemotherapy.
- Published
- 2013
- Full Text
- View/download PDF
90. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells.
- Author
-
Peixoto P, Castronovo V, Matheus N, Polese C, Peulen O, Gonzalez A, Boxus M, Verdin E, Thiry M, Dequiedt F, and Mottet D
- Subjects
- Apoptosis, Autophagy, Cell Cycle Checkpoints, Cell Line, Tumor, DNA Damage, HeLa Cells, Histone Deacetylases chemistry, Histone Deacetylases genetics, Humans, RNA Interference, RNA, Small Interfering metabolism, S Phase, Heterochromatin metabolism, Histone Deacetylases metabolism
- Abstract
Histone deacetylases (HDACs) form a family of enzymes, which have fundamental roles in the epigenetic regulation of gene expression and contribute to the growth, differentiation, and apoptosis of cancer cells. In this study, we further investigated the biological function of HDAC5 in cancer cells. We found HDAC5 is associated with actively replicating pericentric heterochromatin during late S phase. We demonstrated that specific depletion of HDAC5 by RNA interference resulted in profound changes in the heterochromatin structure and slowed down ongoing replication forks. This defect in heterochromatin maintenance and assembly are sensed by DNA damage checkpoint pathways, which triggered cancer cells to autophagy and apoptosis, and arrested their growth both in vitro and in vivo. Finally, we also demonstrated that HDAC5 depletion led to enhanced sensitivity of DNA to DNA-damaging agents, suggesting that heterochromatin de-condensation induced by histone HDAC5 silencing may enhance the efficacy of cytotoxic agents that act by targeting DNA in vitro. Together, these results highlighted for the first time an unrecognized link between HDAC5 and the maintenance/assembly of heterochromatin structure, and demonstrated that its specific inhibition might contribute to increase the efficacy of DNA alteration-based cancer therapies in clinic.
- Published
- 2012
- Full Text
- View/download PDF
91. Urinary neopterin and kynurenine in patients submitted to surgical stress with different inhalational anesthetics (halothane or isoflurane).
- Author
-
Marfella A, Bilancio A, Polese C, Iodice F, Edmondo C, Cerasuolo D, Esposito G, Zannoni V, and Beneduce G
- Subjects
- Adult, Anesthetics, Inhalation therapeutic use, Female, Halothane adverse effects, Halothane therapeutic use, Humans, Hysterectomy adverse effects, Immunosuppressive Agents adverse effects, Immunosuppressive Agents therapeutic use, Isoflurane therapeutic use, Leiomyoma immunology, Leiomyoma surgery, Middle Aged, Stress, Physiological etiology, Stress, Physiological immunology, Uterine Neoplasms immunology, Uterine Neoplasms surgery, Anesthesia, General adverse effects, Anesthetics, Inhalation adverse effects, Isoflurane adverse effects, Kynurenine urine, Neopterin urine, Stress, Physiological urine
- Abstract
Measurements were taken of urinary levels of neopterin (NPT) and kynurenine (KYN), using an HPLC method for their simultaneous analysis in patients submitted to anesthetical surgical stress with two different inhalational anesthetics (halothane and isoflurane). We studied twenty-one women affected by uterine fibromyomatosis and submitted to total hysterectomy (mean age of 42.7+/-5.4 years). They showed the same pre-operative evaluation (ASA-1), and underwent the same i.v. anesthetic treatment. Our patients were randomized in two groups: Group A: 11 patients had halothane as an inhalational anesthetic drug for the maintenance of the anesthetic induction (mean time= 1 h). Group B: 10 patients had isoflurane. A significant decrease in urinary NPT and KYN, parallel to serum-NPT, was found 4 h after anesthetic induction. Raised NPT levels appeared 24 h after A.I. with significant increased levels after 7 days. A strong correlation between urinary and serum NPT levels was seen (Rs= 0.74; p < 0.001). Significantly low KYN levels were observed both 4 h and 24 h after A.I.. In addition to the delayed increase of the excretory KYN levels, significantly raised KYN levels in Group B (isoflurane) 48 h after A.I. (10.59+/-14.31 vs 5.99+/-7.17 micromol/mol creat.; p < 0.01) were shown, whereas in Group A (halothane) we observed a progressive increase as compared to the pre-surgery values starting from 72 h after surgery. Our data seem to show that: (a) it is possible to have a biochemical and non invasive monitoring of the anesthetical-surgical stress on MM "priming" activity; (b) the activation of the phagocyte compartment is one of the earlier immunological events after surgery (NPT), but the efficiency of this "priming" appears to be delayed (KYN); (c) isoflurane appears to induce an earlier recovery in MM activation.
- Published
- 1999
- Full Text
- View/download PDF
92. An HPLC method for the simultaneous analysis of urinary neopterin and kynurenine.
- Author
-
Marfella A, Polese C, d'Alessio P, Beneduce G, Rossi F, and Perna M
- Subjects
- Biopterins urine, Chromatography, High Pressure Liquid, Creatinine urine, Humans, Macrophages drug effects, Neopterin, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Biopterins analogs & derivatives, Kynurenine urine
- Published
- 1992
- Full Text
- View/download PDF
93. Urinary neopterin and kynurenine in herpes gestationis.
- Author
-
Marfella A, Satriano RA, Polese C, Perna M, and Pisani M
- Subjects
- Biopterins urine, Female, Humans, Neopterin, Pregnancy, Biopterins analogs & derivatives, Kynurenine urine, Pemphigoid Gestationis urine
- Published
- 1991
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.