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Fibrils of alpha-synuclein are significant components of cellular inclusions associated with several neuropathological disorders including Parkinson’s disease, multiple system atrophy and dementia with Lewy bodies. In recent years, technological advances in the field of transmission electron microscopy and image processing have made it possible to solve the structure of alpha-synuclein fibrils at high resolution. This review discusses the results of structural studies using cryo-electron microscopy, which revealed that in-vitro produced fibrils vary in diameter from 5 nm for single-protofilament fibrils, to 10 nm for two-protofilament fibrils. In addition, the atomic models hint at contributions of the familial Parkinson’s disease mutation sites to inter-protofilament interaction and the locations where post-translational modifications take place. Here, we propose a nomenclature system that allows identifying the existing alpha-synuclein polymorphs and that will allow to incorporate additional high-resolution structures determined in the future.

The mechanisms of rotavirus entry into the target cell are described as a multi-step event in which the virions are bound to sialic acid (SA), followed by interaction with heat shock cognate protein 70 (Hsc70), some integrins and protein disulfide isomerase (PDI). However, the cell surface receptor molecules facilitating the entry of tumor cell-adapted rotavirus are not completely characterized. Using infection blocking assays with antibodies to some heat shock proteins (HSPs) and also some inhibitors of these cellular proteins, we were able to identify the cell surface Hsp90, Hsp70, Hsc70, Hsp60, Hsp40, PDI and integrin β3 as receptors of tumor cell-adapted rotavirus in Reh cells. Furthermore, the results also indicated that these rotavirus receptors are associated with lipid microdomains (rafts). Our findings provide evidence that rotavirus tropism for these human acute lymphocytic leukemia cells is explained by the relatively high expression of some HSPs in rafts. The results shown here encourage further research aim at evaluating the potential use of rotaviruses as an oncolytic agent for the treatment of some cancers. Keywords: heat shock proteins; rotavirus; cell receptor; cancer; oncolytic virus.

The 235U(n,f) cross section was measured in a wide energy range (18 meV–170 keV) at the n_TOF facility at CERN, relative to 6Li(n,t) and 10B(n,α) standard reactions, with high resolution and accuracy, with a setup based on a stack of six samples and six silicon detectors placed in the neutron beam. In this paper we report on the results in the region between 18 meV and 10 keV neutron energy. A resonance analysis has been performed up to 200 eV, with the code SAMMY. The resulting fission kernels are compared with the ones extracted on the basis of the resonance parameters of the most recent major evaluated data libraries.Acomparison of the n_TOF datawith the evaluated cross sections is also performed from thermal to 10 keV neutron energy for the energy-averaged cross section in energy groups of suitably chosen width. A good agreement, within 0.5%, is found on average between the new results and the latest evaluated data files ENDF/B-VIII.0 and JEFF-3.3, as well as with respect to the broad group average fission cross section established in the framework of the standard working group of IAEA (the so-called reference file). However, some discrepancies, of up to 4%, are still present in some specific energy regions. The newdataset here presented, characterized by a unique combination of high resolution and accuracy, low background and wide energy range, can help to improve the evaluations from the Resolved Resonance Region up to 10 keV, also reducing the uncertainties that affect this region., CERN (European Organization for Nuclear Research)

Unraveling the processes involved in the origin of a substantial fraction of biodiversity can be a particularly difficult task in groups of similar, and often convergent, morphologies. The genus Eriosyce (Cactaceae) might present a greater specific diversity since much of its species richness might be hidden in morphological species complexes. The aim of this study was to investigate species delimitation using the molecular data of the globose cacti “E. curvispina”, which harbor several populations of unclear evolutionary relationships. We ran phylogenetic inferences on 87 taxa of Eriosyce, including nine E. curvispina populations, and by analyzing three plastid noncoding introns, one plastid and one nuclear gene. Additionally, we developed 12 new pairs of nuclear microsatellites to evaluate the population-level genetic structure. We identified four groups that originated in independent cladogenetic events occurring at different temporal depths; these groups presented high genetic diversity, and their populations were genetically structured. These results suggest a complex evolutionary history in the origin of globular cacti, with independent speciation events occurring at different time spans. This cryptic richness is underestimated in the Mediterranean flora of central Chile, and thus unique evolutionary diversity could be overlooked in conservation and management actions.

We report on a hybrid in-beam PET and prompt-gamma Compton imaging system aimed at quasi real-time ion-range verification in proton-therapy treatments. Proof-of-concept experiments were carried out at the radiobiology beam line of the CNA cyclotron facility using a set of two synchronous Compton imagers and different target materials. The time structure of the 18 MeV proton beam was shaped with a series of beam-on and beam-off intervals, thereby mimicking a pulsed proton beam on a long time scale. During beam-on intervals, Compton imaging was performed utilizing the high energy γ-rays promptly emitted from the nuclear reactions occurring in the targets. In the course of the beam-off intervals in situ positron-emission tomography was accomplished with the same imagers using the β decay of activated nuclei. The targets used were stacks of different materials covering also various proton ranges and energies. A systematic study on the performance of these two complementary imaging techniques is reported and the experimental results interpreted on the basis of Monte Carlo calculations. The results demonstrate the possibility to combine both imaging techniques in a concomitant way, where high-efficiency prompt-gamma imaging is complemented with the high spatial accuracy of PET. Empowered by these results we suggest that a pulsed beam with a suitable duty cycle, in conjunction with in situ Compton- and PET-imaging may help to attain complementary information and quasi real-time range monitoring with high accuracy., Open Access funding provided thanks to the CRUE-CSIC agreement with springer nature. This work has been carried out in the framework of a project funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC Consolidator Grant project HYMNS, with grant agreement No.~681740). The authors acknowledge support from the Spanish Ministerio de Ciencia e Innovación under grants PID2019-104714GB-C21, FPA2017-83946-C2-1-P, FIS2015-71688-ERC, FPA2016-77689-C2-1-R, RTI2018-098117-B-C21, CSIC for funding PIE-201750I26 and from the European H2020-847552 (SANDA). This work was partially supported by Generalitat Valenciana PROMETEO/2019/007. J. Balibrea-Correa is supported by grant ICJ220-045122-I funded by MCIN/AEI/10.13039/501100011033 and by European Union NextGenerationEU/PRTR. J. Lerendegui-Marco is supported by grant FJC2020-044688-I funded by MCIN/AEI/10.13039/501100011033 and by European UnionNextGenerationEU/PRTR.

Nanomedicine and the pharmaceutical industry demand the investigation of new biomaterials to improve drug therapies. Combinations of lipids, proteins, and polymers represent innovative platforms for drug delivery. However, little is known about the interactions between such compounds and this knowledge is key to prepare successful drug delivery systems.Biophysical properties of biohybrid vesicles (BhVs) composed of phospholipids, proteins, and amphiphilic block copolymers, assembled without using organic solvents, were investigated by differential scanning calorimetry and dynamic light scattering. We studied four biohybrid systems; two of them included the effect of incorporating tetracaine. Thermal changes of phospholipids and proteins when interacting with the amphiphilic block copolymers and tetracaine were analyzed.Lysozyme and the copolymers adsorb onto the lipid bilayer modifying the phase transition temperature, enthalpy change, and cooperativity. Dynamic light scattering investigations revealed relevant changes in the size and zeta potential of the BhVs. Interestingly, tetracaine, a membrane-active drug, can fluidize or rigidize BhVs.We conclude that positively charged regions of lysozyme are necessary to incorporate the block copolymer chains into the lipid membrane, turning the bilayer into a more rigid system. Electrostatic properties and the hydrophilic-lipophilic balance are determinant for the stability of biohybrid membranes.This investigation provides fundamental information associated with the performance of biohybrid drug delivery systems and can be of practical significance for designing more efficient drug nanocarriers.

Accurate neutron capture cross section data for minor actinides (MAs) are required to estimate the production and transmutation rates of MAs in light water reactors, critical fast reactors like Gen-IV systems, and other innovative reactor systems such as accelerator driven systems (ADS). In particular, 244Cm, 246Cm and 248Cm play a role in the transport, storage and transmutation of the nuclear waste of the current nuclear reactors, due to the contribution of these isotopes to the radiotoxicity, neutron emission, and decay heat in the spent nuclear fuel. Also, capture reactions in these Cm isotopes open the path for the formation of heavier elements. In this work, the results of the capture cross section measurement on 244Cm, 246Cm and 248Cm performed at the CERN n_TOF facility are presented. It is important to notice that the Cm samples used in the experiment at n_TOF have been used previously in an experiment at J-PARC, this experiment and the previous one done in the 70s with a nuclear explosion were the only previous capture experiments for these isotopes. At n_TOF, the capture cross section measurements of 244Cm, 246Cm and 248Cm were performed at the 20 m vertical flight path (EAR2) with three C6D6 total energy detectors. In addition, the cross section of 244Cm was measured at the 185 m flight path (EAR1) with a Total Absorption Calorimeter (TAC). The combination of measurements in EAR1 and EAR2 has contributed to controlling and reducing the systematic uncertainties in the results. The compatibility of the different measurements performed and the techniques to obtain the results are presented in this paper as well as the procedure to obtain the resonance parameters.

The n_TOF neutron time-of-flight facility at CERN is used for nuclear data measurements. The n_TOF Collaboration works closely with the Nuclear Reaction Data Centres (NRDC) network to disseminate the experimental data through the international EXFOR library. In addition, the Collaboration helps integrate the results in the evaluated library projects. The present contribution describes the dissemination status of n_TOF results, their impact on evaluated libraries and ongoing efforts to provide n_TOF resonance parameters in ENDF-6 format for further use by evaluation projects.

The neutron capture cross section of 241Am is an important quantity for nuclear energy production and fuel cycle scenarios. Several measurements have been performed in recent years with the aim to reduce existing uncertainties in evaluated data. Two previous measurements, performed at the 185 m flight-path station EAR1 of the neutron time-of-flight facility n_TOF at CERN, have permitted to substantially extend the resolved resonance region, but suffered in the near-thermal energy range from the unfavorable signal-to-background ratio resulting from the combination of the high radioactivity of 241Am and the rather low thermal neutron flux. The here presented 241Am(n,γ) measurement, performed with C6D6 liquid scintillator gamma detectors at the 20 m flight-path station EAR2 of the n_TOF facility, took advantage of the much higher neutron flux. The current status of the analysis of the data, focussed on the low-energy region, will be described here.

The slow neutron capture process (s-process) is responsible for producing about half of the elemental abundances heavier than iron in the universe. Neutron capture cross sections on stable isotopes are a key nuclear physics input for s-process studies. The 72Ge(n, γ) Maxwellian-Averaged Cross Section (MACS) has an important influence on the production of isotopes between Ge and Zr in the weak s-process in massive stars and so far only theoretical estimations are available. An experiment was carried out at the neutron time-of-flight facility n_TOF at CERN to measure the 72Ge(n, γ) reaction for the first time at stellar neutron energies. The capture measurement was performed using an enriched 72GeO2 sample at a flight path length of 184 m, which provided high neutron energy resolution. The prompt gamma rays produced after neutron capture were detected with a set of liquid scintillation detectors (C6D6). The neutron capture yield is derived from the counting spectra taking into account the neutron flux and the gamma-ray detection efficiency using the Pulse Height Weighting Technique. Over 70 new neutron resonances were identified, providing an improved resolved reaction cross section to calculate experimental MACS values for the first time. The experiment, data analysis and the new MACS results will be presented including their impact on stellar nucleosynthesis, which was investigated using the post-processing nucleosynthesis code mppnp for a 25 solar mass model.

A new measurement of the 235U(n,f) fission cross section was carried out at n_TOF. The experiment covered the neutron energy range from 10 MeV up to 500 MeV, and it used the 1H(n,n) cross section as normalization for the neutron fluence measurement. In this contribution, the measurements and the characterization of the detectors covering the incident energy range up to 150 MeV are discussed.

The measurement of the 235U(n,f) reaction cross section in the neutron energy region 10 MeV to 500 MeV was carried out at the CERN n_TOF facility. The experimental campaign, completed in 2018, provided precise and accurate data on the fission reaction relative to neutron-proton elastic scattering. A description and characterization of the used setup for the simultaneous measurement of fission fragments and neutron flux is reported here.

The M4F project brings together the fusion and fission materials communities working on the prediction of radiation damage production and evolution and their effects on the mechanical behaviour of irradiated ferritic/martensitic (F/M) steels. It is a multidisciplinary project in which several different experimental and computational materials science tools are integrated to understand and model the complex phenomena associated with the formation and evolution of irradiation induced defects and their effects on the macroscopic behaviour of the target materials. In particular the project focuses on two specific aspects: (1) To develop physical understanding and predictive models of the origin and consequences of localised deformation under irradiation in F/M steels; (2) To develop good practices and possibly advance towards the definition of protocols for the use of ion irradiation as a tool to evaluate radiation effects on materials. Nineteen modelling codes across different scales are being used and developed and an experimental validation programme based on the examination of materials irradiated with neutrons and ions is being carried out. The project enters now its 4th year and is close to delivering high-quality results. This paper overviews the work performed so far within the project, highlighting its impact for fission and fusion materials science. Peer Reviewed L. Malerba a,*, M.J. Caturla b, E. Gaganidze c, C. Kaden d, M.J. Konstantinovi´c e, P. Olsson f, C. Robertson g, D. Rodney h, A.M. Ruiz-Moreno i, M. Serrano a, J. Aktaa c, N. Anento j, S. Austin i, A. Bakaev e, J.P. Balbuena b, F. Bergner d, F. Boioli k, M. Boleininger l, G. Bonny e, N. Castin e, J.B. J. Chapman l, P. Chekhonin d, M. Clozel m, B. Devincre k, L. Dupuy g, G. Diego a, S.L. Dudarev l, C.-C. Fu g, R. Gatti k, L. G´el´ebart g, B. G´omez-Ferrer n, D. Gonçalves g, C. Guerrero a, P.M. Gueye n, P. H¨ahner i, S.P. Hannula o, Q. Hayat p, M. Hern´andez-Mayoral a, J. Jagielski m, N. Jennett p, F. Jim´enez a, G. Kapoor d, A. Kraych h, T. Khvan e,q, L. Kurpaska m, A. Kuronen r, N. Kvashin j, O. Libera s, P.-W. Ma l, T. Manninen o, M.-C. Marinica g, S. Merino a, E. Meslin g, F. Mompiou t, F. Mota a, H. Namburi s, C.J. Ortiz a, C. Pareige n, M. Prester u, R.R. Rajakrishnan t, M. Sauzay g, A. Serra j, I. Simonovski i, F. Soisson g, P. Sp¨atig v, D. Tanguy h, D. Terentyev e, M. Trebala o, M. Trochet g, A. Ulbricht d, M.Vallet g, K. Vogel d, T. Yalcinkaya w, J. Zhao r a Centro de Investigaciones Energ´eticas, Medioambientales y Tecnol´ogicas (CIEMAT), Madrid, Spain b Universidad de Alicante, San Vicente del Raspeig, Spain c Karlsruher Institut für Technologie (KIT), Karlsruhe, Germany d Helmholtz-Zentrum Dresden-Rossendorf Ev (HZDR), Rossendorf, Germany e Studiecentrum voor Kernenergie / Centre d’´Etude de l’´Energie Nucl´eaire (SCK CEN), Mol, Belgium f KTH Royal Institute of Technology, Stockholm, Sweden g Universit´e Paris-Saclay, Commissariat `a l’´Energie Atomique et aux ´Energies Alternatives (CEA), Gif-sur-Yvette, France h Institut Lumi`ere Mati`ere (ILM), Centre National de la Recherche Scientifique, Lyon, France i Joint Research Centre (JRC)- European Commission, Petten, the Netherlands j Universitat Polit`ecnica de Catalunya, Barcelona, Spain k Laboratoire d’Etude des Microstructures (LEM), Centre National de la Recherche Scientifique, Chˆatillon, France l United Kingdom Atomic Energy Authority (UKAEA), Culham, UK m Narodowe Centrum Badan Jadrowych (NCBJ), Swierk, Poland n Normandie Univ, UNIROUEN, INSA Rouen, CNRS, Groupe de Physique des Mat´eriaux, 76000 Rouen, France o Aalto University, Espoo, Finland p Coventry University, UK q Universit´e de Li`ege, Belgium r Helsingin Yliopisto, Helsinki, Finland s Centrum Vyzkumu ˇReˇz S.R.O. (CVR), ˇReˇz, Czech Republic t Centre pour l’´Elaboration Elaboration de Mat´eriaux et pour l’´Etude des Structures (CEMES), Centre National de la Recherche Scientifique, Toulouse, France u Institut za Fiziku, Zagreb, Croatia v Paul Scherrer Institut (PSI), Villingen, Switzerland w Middle East Technical University (METU), Ankara, Turkey

This paper is the first of three that overview the main mechanisms that drive the microstructure evolution in Fe alloys under irradiation. It focuses on pure a-Fe and compiles the parameters that describe quantitatively the mobility and stability of point-defects and especially their clusters, including possible reactions and criteria to decide when they should react. These parameters are the result of several years of calculations and application in microstructure evolution models. They are mainly collected from the literature and the parameter choice tries to reconcile different sets of values that, while being in general qualitatively similar, are often quantitatively not coincident. A few calculation results are presented here for the first time to support specific approximations concerning defect properties or features. Since calculations cannot cover all possible defect configurations, the definition of these parameters often requires educated guesses to fill knowledge gaps. These guesses are here listed and discussed whenever relevant. This is therefore a “hands-on” paper that: (i) collects in a single report most microstructure evolution parameters that are found in the literature for irradiated a-Fe, including a discussion of the most important mechanisms at play based on current knowledge; (ii) selects a ready-to-use set that can be employed in microstructure evolution models, such as those based on object kinetic Monte Carlo (OKMC) methods. This work also identifies parameters that are needed, but not known, hopefully prompting corresponding calculations in the future. This work has received funding from the Euratom research and training programme 2014-2018 under grant agreement No. 755039 (M4F project). This research also contributes to the Joint Programme on Nuclear Materials of the European Energy Research Alliance (EERA-JPNM).

High fidelity during protein synthesis is accomplished by aminoacyl-tRNA synthetases (aaRSs). These enzymes ligate an amino acid to a cognate tRNA and have proofreading and editing capabilities that ensure high fidelity. Phenylalanyl-tRNA synthetase (PheRS) preferentially ligates a phenylalanine to a tRNAPhe over the chemically similar tyrosine, which differs from phenylalanine by a single hydroxyl group. In bacteria that undergo exposure to oxidative stress such as Salmonella enterica serovar Typhimurium, tyrosine isomer levels increase due to phenylalanine oxidation. Several residues are oxidized in PheRS and contribute to hyperactive editing, including against mischarged Tyr-tRNAPhe, despite these oxidized residues not being directly implicated in PheRS activity. Here, we solve a 3.6 Å cryo-electron microscopy structure of oxidized S. Typhimurium PheRS. We find that oxidation results in widespread structural rearrangements in the β-subunit editing domain and enlargement of its editing domain. Oxidization also enlarges the phenylalanyl-adenylate binding pocket but to a lesser extent. Together, these changes likely explain why oxidation leads to hyperaccurate editing and decreased misincorporation of tyrosine. Taken together, these results help increase our understanding of the survival of S. Typhimurium during human infection.

La protección del Patrimonio y el Paisaje es una materia de gran relevancia en toda Europa, puesto que en sus múltiples formas encarna la identidad, cultura y saberes de toda su historia. En España, para precisar sus mecanismos, se ha consolidado un completo entramado de normas y regulaciones nacionales, autonómicas y municipales que articulan y gestionan su operar acorde a sus competencias y escala territorial. Sin embargo, y a pesar de este macizo tejido normativo, se ha identificado en Andalucía múltiples incumplimientos y omisiones que ponen a diversos elementos del Patrimonio y el Paisaje en grave riesgo frente al crecimiento urbano. Para evidenciar esto, el presente artículo realiza una revisión de los Planes Generales de Ordenamiento Urbano (PGOU) de tres municipios pertenecientes al área metropolitana de Sevilla; Alcalá del Río, Santiponce y La Rinconada, con el fin de reconocer incumplimientos a la normativa territorial, ambiental y urbanística, y analizar los motivos y riesgos que esto implica. Para ello fue necesario, primero, la revisión de la legislación concerniente, para contrastarla con los últimos PGOU de los casos escogidos, utilizando SIG. Su análisis detallado permitió evidenciar numerosas omisiones e incumplimientos, producto de una deficiente aplicación de las normas y una exigua fiscalización. Situación que genera un riesgo de afección a diversos elementos patrimoniales y paisajísticos, de gran valor para toda España.

Myocilin, a modular multidomain protein, is expressed broadly in the human body but is best known for its presence in the trabecular meshwork extracellular matrix, and myocilin misfolding is associated with glaucoma. Despite progress in comprehending the structure and misfolding of the myocilin olfactomedin domain, the structure and function of full-length myocilin, and contextual changes in glaucoma, remain unknown. Here we expressed and purified milligram-scale quantities of full-length myocilin from suspension mammalian cell culture (Expi293F), enabling molecular characterization in detail not previously accessible. We systematically characterized disulfide-dependent and -independent oligomerization as well as confirmed glycosylation and susceptibility to proteolysis. We identified oligomeric states with glycosylation sites that are inaccessible to enzymatic removal. Low-resolution single particle 2D class averaging from conventional transmission electron microscopy imaging confirms an extended arrangement of tetramers, truncated products consistent with dimers, and a higher-ordered state consistent with octamer. Taken together, our study reveals new myocilin misfolded states and layers of intrinsic heterogeneity, expands our knowledge of olfactomedin-family proteins and lays the foundation for a better molecular understanding of myocilin structure and its still enigmatic biological function.

Mycotoxins have several toxicological implications. In the present study, we evaluate the presence of aflatoxin B1 (AFB1), ochratoxin A (OTA), and fumonisin (FB1) in paddy rice, polished rice, and maize from the fields and markets in Nayarit State (Mexico). The results indicated the presence of AFB1 in 21.21% of paddy rice samples and 11.11% of market maize samples. OTA was present in only 3.03% (one sample) of paddy rice samples. FB1 was detected in 87.50% and 88.88% of maize samples from field and market, respectively. The estimated human exposure was calculated for FB1 using the probable daily intake (PDI), which suggested that FB1 could contribute to the development of diseases through the consumption of contaminated maize. Positive samples indicated that some rice and maize samples were not suitable for human consumption. Further efforts are needed to continue monitoring mycotoxins and update national legislation on mycotoxins accordingly This work was supported by the Consejo Nacional de Ciencia y Tecnolgía (CONACYT) under Grant code SALUD-2017-2-289726.

An overall hydromorphological assessment of the middle to lower Guadalquivir River (southern Spain) was conducted using the Morphological Quality Index (MQI) and a novel extension, the Hydro-Morpho...

The lower Guadalquivir River has been subjected to a series of channel adjustments over the last 250 years, mainly in response to human factors and disturbances. From 1778 to 1918, the results show an oscillation in the sinuosity along the upstream segment and a reduction along the downstream segment in response to artificial cut-offs. The first phase of incision started in approximately the 1840s–1850s, following a previous phase of predominant aggradation probably related to abundant sediment supply caused by deforestation in the catchment. During approximately the last 100 years, notable incision and progressive channel narrowing occurred in both segments, although secondary aggradation (upstream of the dams) and widening (in a portion of the downstream segment) were also observed. The first phase of bed incision migrating upstream is interpreted as a consequence of artificial meander cut-offs, whereas most of the adjustments that occurred during the last 100 years are interpreted as a consequence of the large number of dams in the catchment and along the studied area, which caused a notable deficit in bedload.

The loss of individuals in disturbed sites may be buffered by the presence of small natural features (SNFs), sites acting as ecological refuges preventing the loss of phenotypic variability and genetic diversity of plants. An important group of SNFs is rocky outcrops, singular geological formations that stand out from the surrounding matrix which could host a greater species diversity. We tested the value of rocky outcrops as SNFs of a Tertiary relict tree, the Chilean lucuma Pouteria splendens (Sapotaceae). We identified vegetation patches with P. splendens and characterized their landscape properties in sites associated with rocky outcrops and clearings (plains). Then, we contrasted the genetic diversity, phenotypic variability and sapling abundance of P. splendens inhabiting rocky outcrops and plains. We observed that rocky outcrops sustained subtle higher genetic diversity compared with plains, but non-significant genetic structuration was detected between habitat types. Independently of the habitat type, P. splendens have a significant role in the sapling recruitment: their number was higher underneath of conspecifics than in open spaces. Besides, plants in the rocky outcrops produced smaller and ovoid-shaped fruits, as a possible result of selective pressures generated by frugivorous. We propose that rocky outcrops are regeneration sites for P. splendens. However, subtle differences in genetic diversity between habitats and the lacking of genetic structuration suggest that conservation strategies should be focused on the protection of all habitat types as a manner of maintaining ecosystem processes in this singular vegetation remnant.

Eriosyce is one of most species‐rich genera within Notocacteae (Cactaceae) harboring a variety of stem and flower morphologies, and fruits with basal abscission. The lack of a well‐sampled molecular phylogeny contributes to the current taxonomic instability of the genus, where its circumscription and infrageneric classification has been questioned. Specimens of Eriosyce (63 taxa) plus 19 outgroups were analyzed through sequencing three plastid noncoding introns (rpl32‐trnL, trnL‐trnF, trnH‐psbA), one plastid gene (ycf1), and one nuclear gene (PHYC). Individual markers and concatenated matrices were analyzed using maximum likelihood and Bayesian approaches. Phylogenetic analyses strongly support the monophyly of Eriosyce s.l. Furthermore, seven clades within Eriosyce s.l. were defined based on supported branches, although one of them was weakly supported. Our results suggest that some past taxonomic proposals have low phylogenetic support and should no longer be used, e.g., based on their scattered positions in the phylogenetic reconstruction, several infraspecific taxa appear unrelated to the typical form of the species in which they had been placed. We present a phylogeny‐informed infrageneric classification of the genus Eriosyce, and new combinations are proposed to update the nomenclature of species and sections.

Electronic health records are valuable for clinical and translational research. Institutions must protect patient privacy and comply with applicable regulations while allowing appropriate access to clinical data for research. The processes that investigators must follow to access clinical data can be substantially different at different institutions. In this paper, we describe the process developed at our institution that has been active for 5 years and was used to satisfy over 200 requests for access to identified clinical data, usually within 1 day for internal requests and 3 days for visiting researchers.

Delayed flowering due to later snowmelt and colder temperatures at higher elevations in the alpine are expected to lead to flowering phenological adjustment to prevent decoupling of peak flowering from the warmest time of the year, thereby favoring pollination. However, even if flowering is brought forward in the season at higher elevations, an elevational temperature gap is likely to remain between the high- and low-elevation populations of a species at the time these reach peak flowering on account of the atmospheric reduction in temperature with increasing elevation. The negative effect of this temperature gap on pollination could be compensated by plastically-prolonged flower life spans at higher elevations, increasing the probability of pollination. In a tightly temperature-controlled study, the flowering phenology adjustment and flower longevity compensation hypotheses were investigated in an alpine species in the Andes of central Chile. The snow free period varied from 7 to 8.2 months over 810 m elevation. Temperatures were suitable for growth on 82–98% of the snow free days. Flowering onset was temporally displaced at the rate of 4.6 d per 100 m increase in elevation and flowering was more synchronous at higher elevations. Flowering phenology was adjusted over elevation. The latter was manifest in thermal sums tending to decrease with elevation for population flowering onset, 50% flowering, and peak flowering when the lower thermal limit for growth (TBASE) was held constant over elevation. For TBASE graded over elevation so as to reflect the growing season temperature decline, thermal sums did not vary with elevation, opening the door to a possible elevational decline in the thermal temperature threshold for growth. Potential flower longevity was reduced by passive warming and was more prolonged in natural populations when temperatures were lower, indicating a plastic trait. Pollination rates, as evaluated with the Relative Pollination Rate index (RPR), when weighted for differences in floral abundance over the flowering season, declined with elevation as did fruit set. Contrary to expectation, the life-spans of flowers at higher elevations were not more prolonged and failed to compensate for the elevational decrease in pollination rates. Although strong evidence for phenological adjustment was forthcoming, flower longevity compensation did not occur over Oxalis squamata´s elevational range. Thus, flower longevity compensation is not applicable in all alpine species. Comparison with work conducted several decades ago on the same species in the same area provides valuable clues regarding the effects of climate change on flowering phenology and fitness in the central Chilean alpine where temperatures have been increasing and winter snow accumulation has been declining.

Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments. C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption. It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion. Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption. Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency. Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO. To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament. We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans. The possibility of interplay between these modifications and ϕCbK adsorption is discussed. We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 A. Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level. IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria. Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages. This study characterizes the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK. We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK. We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.

This data paper presents a largely phylogeny-based online taxonomic backbone for the Cactaceae compiled from literature and online sources using the tools of the EDIT Platform for Cybertaxonomy. The data will form a contribution of the Caryophyllales Network for the World Flora Online and serve as the base for further integration of research results from the systematic research community. The final aim is to treat all effectively published scientific names in the family. The checklist includes 150 accepted genera, 1851 accepted species, 91 hybrids, 746 infraspecific taxa (458 heterotypic, 288 with autonyms), 17,932 synonyms of accepted taxa, 16 definitely excluded names, 389 names of uncertain application, 672 unresolved names and 454 names belonging to (probably artificial) named hybrids, totalling 22,275 names. The process of compiling this database is described and further editorial rules for the compilation of the taxonomic backbone for the Caryophyllales Network are proposed. A checklist depicting the current state of the taxonomic backbone is provided as supplemental material. All results are also available online on the website of the Caryophyllales Network and will be constantly updated and expanded in the future. Citation: Korotkova N., Aquino D., Arias S., Eggli U., Franck A., Gomez-Hinostrosa C., Guerrero P. C., Hernandez H. M., Kohlbecker A., Kohler M., Luther K., Majure L. C., Muller A., Metzing D., Nyffeler R., Sanchez D., Schlumpberger B. & Berendsohn W. G. 2021: Cactaceae at Caryophyllales.org – a dynamic online species-level taxonomic backbone for the family. – Willdenowia 51: 251–270. Version of record first published online on 31 August 2021 ahead of inclusion in August 2021 issue. Data published through: http://caryophyllales.org/cactaceae/Checklist

The increasing demand of natural products for the control of plant diseases has led to the search for biological control agents, namely fungi, often isolated from composts of organic residues. By composting two different mixes of agro-industrial residues, P1 and P2 composts were obtained, from where a known antagonist, Trichoderma atroviride, was isolated. Later, a second composting process was performed and when the composting mix reached room temperature, the previously isolated T. atroviride was inoculated in both composts to enrich its population (E) and named as P1E and P2E. The suppressive capacity of these two composts against Sclerotium rolfsii, Clarireedia spp. and Rhizoctonia solani was tested two weeks after T. atroviride inoculation, in 100 mL pots with turfgrass seeded with Agrostis stolonifera. The tested treatments were: composts P1 and P2 without any treatment; thermally treated P1 and P2 (P1t, P2t); P1 and P2 enriched without and with previous thermic treatment (P1E, P2E, P1tE, P2tE), and a commercial peat-based substrate, natural (P) and thermally treated (Pt), enriched (PE) and enriched after thermal treatment (PtE). Enriched composts achieved the highest disease control. P1E was more effective in controlling Clarireedia spp.; pots with infected plants grown on P1E, showed 53.5% less affected area compared to P1t. P2E was more effective against R. solani; results showed 69.3% less affected area compared to P2t and both were effective in S. rolfsii control, with a reduction of 38.5% in P1E and 43.5% in P2E. The larger population of Trichoderma spp. observed in the enriched substrates associated to the greater enzyme activity, namely and N-acetyl-β-glucosaminidase, standing out in P2E, will have contributed strongly to the biocontrol of the studied diseases. An increase in some macro and micronutrients was observed in the enriched heat-treated substrates. FCT: SFRH/BD/109218/2015 info:eu-repo/semantics/publishedVersion

Se estudia la distribución espacial de los usos del territorio y de pérdida de suelo en la subcuenca hidrográfica del Oued Ourtza (Pre-Rif Oriental, Marruecos) entre los años 2000 y 2014. Para ello, se ha recurrido a información extraída de imágenes de satélite, el empleo de la ecuación universal de pérdida de suelo en su versión adaptada a las condiciones mediterráneas (RUSLE) y el correspondiente tratamiento mediante herramientas SIG. Las cartografías resultantes denotan la sustitución de los cultivos tradicionales de cereal por otros arbóreos y un importante abandono de las tierras (36,8%). Las pérdidas de suelo son bastante altas (40 t·ha-1·año-1), muy por encima de su tolerancia, aunque están cercanas a las tasas de ablación registradas en otras áreas del entorno mediterráneo y el Rif marroquí. Estas pérdidas se distribuyen desigualmente, correspondiendo las tasas más elevadas con las tierras no cultivadas o abandonadas (>80 t·ha-1 ·año-1) cuya extensión sigue aumentando, sobre todo, en los tramos medio y bajo de la subcuenca donde dominan los materiales lábiles. The spatial distribution of land use and soil loss in the hydrographic sub-basin of the Oued Ourtza (Eastern Prerif, Morocco) between 2000 and 2014 is the subject of this study. We have extracted the thematic information from satellite imagery for the mapping of land occupation and then used geographic information systems for the spatialisation of water erosion according to the universal equation for soil loss in its version adapted to Mediterranean conditions. The resulting maps show an increase in the areas of arboriculture and a significant abandonment of land (36.8 %). The soil losses are quite high (40 t·ha-1·yr-1) and well above the tolerance threshold; although they are similar to ablation rates in some areas of the Mediterranean environment and the Moroccan Rif. These losses are unevenly distributed, with the highest rates corresponding to uncultivated or abandoned land (>80 t·ha-1 ·yr-1) whose extension continues to increase, especially in the middle and lower reaches of the sub-basin where fragile materials dominate.

In this paper, we present sufficient conditions for the stochastic stability (SS) of a Markov jump linear singular system (MJLSS) with partially known transition probabilities. To handle this problem, we give two different approaches: the first one is based on the Dynamics decomposition (DD) of the system and the second one on the Weierstrass decomposition (WD). We show the relationship between these two approaches and provide a numerical example. By using the MATLAB Econometrics Toolbox, a simulation is run in order to validate our results.

Background Self-care is a crucial factor in the education of patients with heart failure (HF) and directly impacts in the progression of the disease. However, little is published about its major clinical implications as admission or mortality in patients with HF. Aims and methods The aim of the study was to analyze time to admission due to acute heart failure and mortality associated with poor self-care in patients with chronic HF. We prospectively recruited consecutive patients with stable chronic HF referred to a nurse-led HF programme. Selfcare was evaluated at baseline with the 9 item European Heart Failure Self-Care Behavior Scale. Scores were standardized and reversed from 0 (worst selfcare) to 100 (better self care). For the purpose of this study we analyzed the associations of worse self-care (defined as scores below the lower tertile of the scale) with demographic, disease-related (clinical) and psychosocial factors in all patients at baseline. Results We included 1123 patients, mean age 72±11, 639 (60%) were male, mean LVEF 45±17 and 454 (40,4%) were in NYHA class III or IV. Mean score of the 9-item ESCBE was 69±28. Score below 55 (lower tertile) defined impaired selfcare behaviour. Those patients with worse self-care had more ischaemic heart disease, more COPD, and they achieved less distance in the 6 minute walking test. Regarding psychosocial items patients in lower tertile of self-care needed a caregiver more frequently, they present more cognitive impairment, depressive symptoms and worse score in terms of health self-perception. Multivariate Cox Models showed that a score below 55 points in 9-item ESCBE was independently associated with higher readmission due to acute heart failure [HR 1.26 (1.02–1.57), p value=0.034] and with mortality [HR 1.24 CI95% (1.02–1.50), p value=0.028] Conclusion Poor self-care measured with the modified 9-item ESCBE was associated with higher risk of admission due to acute decompensation and higher risk of mortality in patients with chronic heart failure. These results highlight the importance of assessing self-care and provide measures to improve them. Funding Acknowledgement Type of funding source: Public hospital(s). Main funding source(s): Hospital Univesitario de Bellvitge

Background Reduction of readmissions in heart failure (HF) patients is a main goal of HF programmes. Establishing a discharge planning for the patient and coordinating it with primary care teams are key aspects for their success. Purpose Evaluate whether a double check discharge planning based on adding face-to-face joint weekly sessions with primary care managers to the conventional electronic communication of care plan reduces 6-month readmission and 6-month mortality. Methods We evaluated all patients discharged from hospital with HF as primary diagnosis between September 2017 and January 2019. We compared outcomes between patients discharged during Period #1 (single check; September 2017 - April 2018) and those discharged during Period #2 (double check; May 2018 - January 2019). Primary endpoint was the combined endpoint of all-cause death or all-cause hospitalization 6 months after discharge from the index hospitalization. Results The study enrolled 317 patients: 182 in Period #1 and 135 in Period #2. Mean age was 76±9 years. There was a higher proportion of patients with diabetes and COPD in Period #1, with no differences in other baseline characteristics. The combined endpoint of all cause-death and all-cause hospitalization at 6 months was significantly reduced in patients in the double check discharge planning group (27% vs. 16%, p 0.021). Conclusions In a HF programme, the addition of a double check discharge planning based on having joint weekly sessions with primary care managers on top of the conventional electronic communication of care plan reduces 6-month readmission and 6-month mortality. Funding Acknowledgement Type of funding source: Public hospital(s). Main funding source(s): Hospital Universitari de Bellvitge

Background Self care is a crucial factor in the education of patients with heart failure (HF) and directly impacts in the progression of the disease. Beyond heart failure related factors, the role of psychosocial determinants and its interaction with clinical aspects has not been fully explored. Aims The aim of the study was to analyze both clinical and psychosocial factors associated with poor self care in patients with chronic HF. Methods Self care was evaluated at baseline with the 9 item European HFR Self Care Behaviour Scale (9 item ESCBS). Scores were standardized and reversed from 0 (worst self care) to 100 (better self care). This study we analysed the associations between poor self care (defined as scores in the lower tertile of the 9 item ESCBS) with demographic, HF-related (clinical) and psychosocial factors in all patients at baseline. Results We included 1123 patients: mean age 72±11, 639 (60%) were male, mean LVEF 45±17 and 454 (40%) were in NYHA class III or IV. Mean score of the 9-item ESCBS was 69±28. In the clinical multivariate analyses HF-related factors associated with poor self-care were serum albumins level, ckd level and previous admission due to heart failure. In the psychosocial multivariate analyses poor social support, depressive symptoms and needing a caregiver were independently associated with poor self care. In combined models, only psychosocial factors were independently associated with poor self care whereas no clinical factors remain in the model. Conclusion Our study showed that psychosocial conditions are the main factors independently associated with poor self-care in patients with chronic heart failure Funding Acknowledgement Type of funding source: Public hospital(s). Main funding source(s): Hospital del Bellvitge

Background Reduction of 30-day readmission in heart failure (HF) patients is a main goal of health-care systems. Programmes to decrease 30-day readmission have successfully reduced it but have failed to neither maintain benefit afterwards nor decrease mortality. Moreover, in many cases the price of reducing 30-day readmission is a mortality increase. Purpose Evaluate whether the impact of a fully nurse-led HF programme directed to reduce 30-day readmission and mortality extends to longer periods of time, including 90 days and 180 days after discharge. Methods We evaluated all patients discharged from hospital with HF as primary diagnosis between January 2017 and January 2019. We compared outcomes between patients discharged during Period #1 (pre-programme; Jan 2017 - Aug 2017) and those discharged during Period #2 (HF programme; Sept 2017 - Jan 2019). Primary endpoint was the combined endpoint of all-cause death or all-cause hospitalization 90 days and 180 days after discharge from the index hospitalization. Results The study enrolled 440 patients: 123 in Period #1 and 317 in Period #2. Mean age was 75±9 years. There was a higher proportion of female patients in Period #2 (38.2% vs 26.8%, p=0.025), with no differences in other baseline characteristics. The combined endpoint of all cause-death and all-cause hospitalization was significantly reduced in patients in the HF programme group, both at 90 days [OR 0.37 (0.22–0.63), p There were no differences between groups in mortality [OR 0.96 (0.18–5.00), p=0.293]. Conclusions A fully nurse-led HF programme reduces the combined endpoint of all-cause death and all-cause hospitalization both at 90 days and 180 days after an index discharge for HF. Such a decrease is driven by a reduction of CV and HF hospitalization, which are maintained over time. There were no differences between groups in mortality. Funding Acknowledgement Type of funding source: Public hospital(s). Main funding source(s): Hospital Universitari de Bellvitge

Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments. C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption. It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion. Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption. Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency. Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO. To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament. We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans. The possibility of interplay between these modifications and ϕCbK adsorption is discussed. We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å. Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level. IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria. Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages. This study characterizes the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK. We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK. We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.

The use of pig deep-litter (PDL) in pasture fertilization can be an important alternative from an environmental and economic point of view. This study was conducted to evaluate the fertilization with PDL exclusively or in association with mineral fertilizers on the quality and productivity of Panicum maximum cv. Mombasa. The experiment was carried out in Ipameri, GO, Brazil. The design was a randomized block design in a 2 x 5 factorial scheme with four repetitions, in plots of 24 m². The doses of PDL (0; 5; 10; 15 and 20 Mg ha-1) were tested, with or without fertilization with NPK (50 kg ha-1 of P2O5, 10 kg ha-1 of K2O and 10 kg ha-1 of N). The organic fertilizer was applied broadcast at the planting of the pasture, and the mineral fertilizers were split and applied at planting and as topdressing. Dry mass, crude protein, acid detergent fiber and neutral detergent fiber were evaluated. PDL doses associated with mineral fertilization led to linear increases from 52 to 282% in the analyzed variables and better bromatological composition. PDL application is beneficial to the production of pastures, and increasing applications up to the PDL dose of 20 Mg ha-1 associated with mineral fertilization promoted better quantitative and qualitative results. With exclusive fertilization with PDL, the gains in quality and productivity were more modest. RESUMO O uso de cama sobreposta de suínos (CSS) na adubação de pastagens pode ser uma importante alternativa do ponto de vista ambiental e econômico. Com o objetivo de avaliar a adubação com CSS de forma exclusiva ou associada a fertilizantes minerais, na qualidade e produtividade de Panicum maximum cv. Mombaça, foi conduzido um experimento em condições de campo, em Ipameri, GO. O delineamento foi em blocos ao acaso em esquema fatorial 2 x 5 com quatro repetições, em parcelas de 24 m². Foram testadas as doses de CSS (0; 5; 10; 15 e 20 Mg ha-1), com ou sem adubação com NPK (50 kg ha-1 de P2O5, 10 kg ha-1 de K2O e 10 kg ha-1 de N). O adubo orgânico foi aplicado a lanço na implantação da pastagem, e os fertilizantes minerais parcelados no plantio e em cobertura. Foram avaliadas a massa seca, proteína bruta, fibra em detergente ácido e fibra em detergente neutro. As doses de CSS associadas à adubação mineral apresentaram incrementos lineares de 52 a 282% nas variáveis avaliadas, e melhor composição bromatológica. A aplicação de CSS é benéfica à produção de pastagens e as aplicações crescentes até à dose de 20 Mg ha-1 de CSS associadas à adubação mineral apresentaram melhores resultados quantitativos e qualitativos. Com a adubação exclusiva com CSS os ganhos de qualidade e produtividade do capim Mombaça são menores.

Algarve region (Portugal) has nearly 40 golf courses with a significant economic impact. Summer surveys on 10 golf courses detected an unknown disease on one course in 2009 and on another course in 2012 and 2013 at 29 to 30°C daily average. The second course had symptoms on about 25% of the turf of two putting greens. Diseased bentgrass (Agrostis stolonifera L.) had a green dappled pattern with irregular patches of turfgrass on yellowed leaves. Prior to decaying, affected leaves turned brown and then gray. Crown and leaf sheath infections resulted in dark brown dry rot. No lesions were observed on the roots. Leaves were surface disinfected with 5% commercial bleach (0.225% sodium hypochlorite) and cultured on potato dextrose agar (PDA). Ten fungal colonies grew from the leaf tissue, and brown mycelia, conidiophores, and conidia were observed under a microscope. Conidia were ventricose pyriform, mostly abruptly curved, 20 to 36 µm (30 µm, SD = 4) × 7 to 12 µm (10.5 µm, SD = 1.3) (n = 50), predominantly three-septate, with a prominent hilum and enlarged and darkened central cells. Colonies grown on PDA were black-brown with a black reverse side. Conidia differed in size, 15.4 to 24.6 µm (19.99 µm, SD = 3.00) × 6 to 11 μm (8.68 µm, SD = 1.54) (n = 50) and morphology (cylindrical or slightly curved). These characteristics were consistent with Curvularia trifolii (Kauffm.) Boedijn. (Ellis 1971; Falloon 1976; Khadka 2016). Species identification of the representative isolate A2 1.12 was confirmed by analysis of nucleotide sequences of the ITS1-5.8S-ITS2 region using primers ITS1 and ITS4 (White et al. 1990) and GPDH gene region with primer set gpd (Koike et al. 2013). BLAST searches of GenBank showed a high similarity of the isolate ITS sequence (MG029439) to the reference sequence JN712458 of C. trifolii (99% identity) and GPDH sequence (MK570108) with LT715803.1 (97.88% identity). The maximum likelihood phylogenetic tree showed that our isolate clustered with C. trifolii. The pathogenicity assay of this isolate was conducted in greenhouse on A. stolonifera ‘Penncross’. The isolate was grown on PDA (25°C, 10 days). Five pots (100 ml) were filled with a sand and peat mix (9:1 v/v) with 0.06 g of seeds per pot, covered with a fine sand layer. Turfgrass was cut once a week beginning 2 weeks after seeding and was fertigated with 0.5 g/liter of Peter’s foliar feed (27 + 15 + 12; N + P2O5 + K2O; and micronutrients; Scotts, Heerlen, The Netherlands). To obtain a conidial suspension for inoculation, cultured plates were scraped with a sterilized spreader and water. The suspension was filtered through a sterile gauze. Conidia were counted under a microscope (400×) with a hemocytometer. The suspension was adjusted to 8 × 103 conidia/ml, and 10 ml was sprayed per pot. Pots maintained humidity for 2 days under microtunnels. The first disease symptoms appeared 3 days after inoculation. Bentgrass from the five pots developed Curvularia blight and rotted crown symptoms. Control plants (five pots treated with water) did not display symptoms. This trial was repeated once. On PDA, C. trifolii was reisolated from leaf lesions and morphologically identified, confirming Koch’s postulates. Ellis (1971) referred to the presence of C. trifolii in Portugal, but no region, symptom description, or grass species was detailed. Sivanesan (1987) reported C. trifolii in Portugal only on Lolium multiflorum. Therefore, this is the first report of C. trifolii in Algarve, affecting A. stolonifera. This disease can increase maintenance costs in greens in this area. info:eu-repo/semantics/publishedVersion

CartujaQanat represents the necessity to rethink the way our cities are conceived as time passes by and the world evolves. Current and future needs demand up-to-date solutions that combine the knowledge obtained from experience and tradition with innovative research. Cities have the responsibility to contribute to a better environment for their inhabitants while minimizing resource consumption. Passive techniques and bioclimatic solutions have significant benefits for the health and well-being of humans, making them ideal when properly adapted to their suitable climates. Keeping this in mind, CartujaQanat pursues the creation of open-air spaces that provide a comfortable environment in hot and dry weather conditions with minimal negative impacts. Strategies developed for the project utilize water as a heat transfer fluid in open and closed systems to enable the acclimatization of exterior spaces. The project includes the use of physical barriers in the form of bioclimatic lattices that act as a solar screen, enabling natural ventilation and providing a certain level of confinement to the air. These effects are enhanced with the hygrothermal capabilities of water, which is incorporated for direct and indirect evaporative cooling as well as thermally activated elements.