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Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) is a human-oncogenic herpesvirus. Cells from KSHV-associated tumors, such as Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL), are of endothelial and B-cell origin, respectively. KSHV persists indefinitely in these cell lineages during latent infection. Indeed, cellular latency is a hallmark of all herpesviruses that is intimately linked to their pathogenesis. We previously characterized the promoter for the KSHV latency-associated nuclear antigen LANA/orf73. LANA is required for latent episome maintenance and has also been implicated in oncogenesis. Hence, regulation of LANA expression is critical to KSHV persistence. We find that a region extending to bp -1299 upstream of the LANA transcription start site is able to drive lacZ-reporter gene expression in several lines of transgenic mice. In agreement with KSHV's natural tropism, we detected reporter gene expression in CD19-positive B cells but not in CD3-positive T cells. We also detected expression in the kidney and, at a lower level, in the liver. In contrast to KS tumors, transgene expression was localized to kidney tubular epithelium rather than vascular endothelial cells. This suggests that our promoter fragment contains all cis-regulatory elements sufficient for B-cell specificity but not those required for endothelial specificity. Alternatively, while the trans-acting factors required for LANA expression in B cells are evolutionarily conserved, those that regulate endothelial cell-specific expression are unique to humans. Our in vivo studies address a conundrum in KSHV biology: in culture, KSHV is able to infect a variety of cell types indiscriminately, while in healthy latent carriers KSHV is found in B lymphocytes. The transgenic-mouse experiments reported here suggest that tissue-restricted LANA gene expression could explain B-cell-specific viral persistence.

A risk assessment comparing the acute effects of mineral oil and PCB-askarel dielectric fluids in two transformer sites was performed. The first site has the installation characteristics for a PCB-askarel-filled transformer with sprinkler fire protection. A risk comparison is made between two types of transformers (PCB-askarel-filled and mineral oil-filled) for this site. The second site (a vault) has the installation characteristics for a mineral oil-filled transformer, and a risk comparison is made in a fashion similar to the first site. Risk is expressed in terms of frequencies of one or more acute injuries or fatalities per transformer year.

Although the quality of observational data is generally evaluated by observer agreement, measures of both observer agreement and accuracy were available in the present study. Videotapes with a criterion protocol were coded by 16 observers. All observers calculated agreement scores both on their own and their partner's data and on a contrived data set misrepresented as data collected by other observers. Compared with agreement scores calculated by the experimenter, observers erroneously inflated their own agreement scores and deflated the agreement scores on the contrived data. Half of the observers (n = 8) had been given instructions emphasizing the importance of accuracy during observation while the other half had been given instructions emphasizing interobserver agreement. Accuracy exceeded agreement for the former group, whereas agreement exceeded accuracy for the latter group. The implications are that agreement should be calculated by the experimenter and that the accuracy-agreement relationship can be altered by differential observer instructions.