Search

The use of illicit substances continues to pose a substantial threat to global health, affecting millions of individuals annually. Evidence suggests the existence of a 'brain–gut axis' as the involving connection between the central nervous system and gut microbiome (GM). Dysbiosis of the GM has been associated with the pathogenesis of various chronic diseases, including metabolic, malignant, and inflammatory conditions. However, little is currently known about the involvement of this axis in modulating the GM in response to psychoactive substances. In this study, we investigated the effect of MDMA (3,4-methylenedioxymethamphetamine, "Ecstasy")-dependence on the behavioral and biochemical responses, and the diversity and abundance of the gut microbiome in rats post-treated (or not) with aqueous extract of Anacyclus pyrethrum (AEAP), which has been reported to exhibit anticonvulsant activity. The dependency was validated using the conditioned place preference (CPP) paradigm, behavioral, and biochemical tests, while the gut microbiota was identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). The CPP and behavioral tests confirmed the presence of MDMA withdrawal syndrome. Interestingly, treatment with AEAP led to a compositional shift in the GM compared to the MDMA-treated rats. Specifically, the AEAP group yielded a higher relative abundance of Lactobacillus and Bifidobacter, while animals receiving MDMA had higher levels of E. coli. These findings suggest that A. pyrethrum therapy may directly modulate the gut microbiome, highlighting a potential target for regulating and treating substance use disorders. [ABSTRACT FROM AUTHOR]

Cutaneous leishmaniasis is a neglected skin disease that poses a serious health problem in many countries including Morocco. Although treatment is free of charge at state health facilities, there is under-utilization of health services by patients. The objective is to know and understand the obstacles faced by people with cutaneous leishmaniasis when seeking anti-Leishmania treatment. Qualitative study was conducted among people affected by cutaneous leishmaniasis in central Morocco in rural communes in the province of El Hajeb. In-depth individual interviews were conducted and transcribed verbatim. A thematic analysis was used to process and present these data. In total, fourteen people with cutaneous leishmaniasis were recruited with an average age of 45 years, 78.6 % of whom lived in rural areas. Four main themes emerged from the data analysis: (1) Perception of the disease and trivialization of skin lesions; (2) Popular treatmentseeking behavior; (3) Gender and decision to seek treatment; (4) Therapeutic preferences and refusal of injection. This research showed that patients did not seek medical treatment for their disease until the lesions persisted despite the application of traditional remedies. Socio-cultural aspects were the most important factors preventing patients from seeking and receiving timely medical care. The female gender was a determinant for seeking health care from caregivers. The trivialization of skin lesions and the use of traditional practices were socio-cultural factors limiting the use of medical treatment by those affected. These factors, together with the fear of pain caused by the injection, were the decisive point for abandoning anti-Leishmania treatment. [ABSTRACT FROM AUTHOR]

Sand flies are the exclusive vectors of leishmaniasis. This group of parasitic diseases is a serious public health problem in Morocco. The aim of this study was to investigate the sand fly fauna, mainly the species composition, biodiversity, and seasonal activity of sand flies in El Hajeb in central Morocco. A total of six stations (Aït Naaman, Aït Rbaa, Aït Brahim, Ain Taoujdate, Sidi Mbarek, and Aït Oufella) were studied, five of which had recently recorded cases of leishmaniasis. Sand fly bimonthly captures were carried out using a sticky paper trap in different biotopes from March to December 2019. A total of 14590 adult sand flies were collected. The activity of the sand fly started in April and declined in November. The periods of high abundance were July, September, and November. Morphological identification of sand flies shows the presence of twelve species: Phlebotomus papatasi, P. longicuspis, P. perniciosus, P. sergenti, P. bergeroti, P. alexandri, P. dreyfussi, P. ariasi, Sergentomyia fallax, S. minuta, S. schwetzi, and S. antennata. The analysis showed that species belonging to the genus Phlebotomus were the most dominant (93.3%) and the risk periods were spread during the summer and autumn seasons. The present study provides for the first time information on the species of sand flies in El Hajeb. It, therefore, provides decision makers with an important tool to conduct vector control actions during peak periods in order to limit the transmission of leishmaniasis. A preprint was made available by the research square in the following link: "https://assets.researchsquare.com/files/rs-1409330/v1/dfef7013-0327-4a54-897f-214924a2d950.pdf?c=1646838874." [ABSTRACT FROM AUTHOR]

Since the introduction of active teaching methods, health science education has undergone considerable transformation. These methods help students become more motivated and develop communication, analytical and critical thinking skills, which influence students' learning practices.The objective of this study is to evaluate effects of role-play simulation-based case studies on motivation and learning strategies among health sciences.One hundred sixty-two students participated in this randomized trial. Students in the control group (n = 86) attended traditional interactive lectures, whereas students in the experimental group (n = 76) participated in role-play simulation-based case studies. The Motivated Strategies for Learning Questionnaire (MSLQ) was administered both before and after educational intervention for both groups.Compared to the control group, the scores of the experimental group are higher for Intrinsic goal orientation, Task value, Self-efficacy for learning and performance, Metacognitive self-regulation, Elaboration and critical thinking, Peer learning and help seeking (p< 0,001). In addition, we observed a positive association between role-play simulation-based case studies and Post-test sore of MLSQ for Task value, Self-efficacy for learning and performance, and Peer learning and help seeking (p < 0.05).The study findings support the use of role-play simulation-based case studies in improving the motivation significantly and changing learning strategies for health care students. This active method can be recommended as a promising method for teaching health science. [ABSTRACT FROM AUTHOR]

Introduction: there is a high proportion of novice midwives assigned to healthcare centers located in rural area difficult to access geographically. To improve professional communication and professionalization of these novice midwives, e-mentoring is proposed as a solution to consolidate support of the novice midwives. The aim of our research is to explore the feasibility and the acceptability of an e-mentoring professional coaching model of midwives in our geographic and professional context. Methods: an exploratory study targeted the Newly Recruited Midwives (NRMW) having less than two years of practice (N = 31) in northern Morocco. The model is inserted in a "Slack" free software where two mentors admitted the NRMW who consented to participate (N=21) in our study. The mentors ensured a synchronous and asynchronous assistance to the participants in their workplace through this platform. A questionnaire was self-administered by the participants in the first meeting. It assessed the professional skills of the participants and their coaching and training needs. The mentors shared documents and set activities in the channels of the platform. Public channels and forums have been launched. The model lasted from April 22 to May 22, 2019. Results: participation rate was 67.7% (21/31). The interactivity with the platform was uninterrupted through the period test. A high level of digital familiarization was noted. A proportion of 95% expressed the willingness to participate in an e-mentoring professional coaching model associated with a remote mentor permanently. Conclusion: the continuous interactivity of novice midwives with the e-mentoring model showed that it is feasible and acceptable in our context. Such model will solve the problems experienced by midwives in their workplace without delay or additive fees. [ABSTRACT FROM AUTHOR]

It is commonly established that warm blooded animals are extremely vulnerable to the infectious coccidian parasite, Toxoplasma gondii. T. gondii can be transmitted to poultry and livestock through the ingestion of oocyst (fecal-oral), and placenta (congenital toxoplasmosis) in livestock. Cattle, sheep, and rabbits are some of the most consumed livestock in Palestine. As for poultry, backyard chicken, caged chicken, and caged turkeys are some of the most consumed birds. The aim of the present study is to detect the seroprevalence of Anti-T. gondii antibodies (IgG/IgM) among the frequently consumed livestock and poultry, which is collected from farms and slaughterhouses in Gaza, Palestine. For this specific study, blood samples from 213 cattle, 100 sheep, and 99 rabbits were collected. On the other hand, 50 blood samples from each type of poultry mentioned above were also collected. The criteria of samples collection based on a quick survey was performed among animal breeders and slaughterhouses owners in the study area, under the guidance of the colleagues in the Palestinian Ministry of Agriculture (veterinary department), as well as with the support of documented references as the targeted animal groups are the most suitable for meat production regardless of age and gender. The collected samples were analyzed through the use of the ELISA technique and Rapid Cassette Test. The results of the present study showed a high seroprevalence of Anti-T. gondii antibodies among sheep, which was 64.00 % and 13.14% among cattle. On the other hand, the results among backyard chicken and caged chicken were 26.00% and 2.00%, respectively. However, no antibodies were detected in the serum of rabbits and caged turkeys. [ABSTRACT FROM AUTHOR]

Sand flies are the exclusive vectors of leishmaniasis. This group of parasitic diseases is a serious public health problem in Morocco. The aim of this study was to investigate the sand fly fauna, mainly the species composition, biodiversity, and seasonal activity of sand flies in El Hajeb in central Morocco. A total of six stations (Aït Naaman, Aït Rbaa, Aït Brahim, Ain Taoujdate, Sidi Mbarek, and Aït Oufella) were studied, five of which had recently recorded cases of leishmaniasis. Sand fly bimonthly captures were carried out using a sticky paper trap in different biotopes from March to December 2019. A total of 14590 adult sand flies were collected. The activity of the sand fly started in April and declined in November. The periods of high abundance were July, September, and November. Morphological identification of sand flies shows the presence of twelve species: Phlebotomus papatasi , P. longicuspis , P. perniciosus , P. sergenti , P. bergeroti , P. alexandri , P. dreyfussi , P. ariasi , Sergentomyia fallax , S. minuta , S. schwetzi , and S. antennata . The analysis showed that species belonging to the genus Phlebotomus were the most dominant (93.3%) and the risk periods were spread during the summer and autumn seasons. The present study provides for the first time information on the species of sand flies in El Hajeb. It, therefore, provides decision makers with an important tool to conduct vector control actions during peak periods in order to limit the transmission of leishmaniasis. A preprint was made available by the research square in the following link: "https://assets.researchsquare.com/files/rs-1409330/v1/dfef7013-0327-4a54-897f-214924a2d950.pdf?c=1646838874.", Competing Interests: The authors declare that there are no conflicts of interest., (Copyright © 2023 Karima El-Mouhdi et al.)