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54. Apoptosis and pAKT levels in cumulus cells of patients undergoing in vitro fertilization program with specific polymorphisms of gonadotropins and their receptors: a case-control study

Author

Bosco, L., Roccheri, M., Paffoni, A., Piomboni, P., Valerio, D., Sarcina, E., Lispi, M., Ruvolo, G., Bosco, L, Roccheri, MC, Paffoni, A, Piomboni, P, Valerio, D, Sarcina, E, Lispi, M, and Ruvolo, G.

Subjects
Apoptosis, polymorphism, oocytes, Settore BIO/06 - Anatomia Comparata E Citologia
Abstract

Study question Is there a difference in oocyte competence among patients with different gonadotrophin polymorphisms, after ovarian stimulation with r-FSH? Summary Answer Higher DNA Fragmentation Index and cleaved caspase-3 related to lower level of pAKT has been observed in patients with specific gonadotrophin polymorphism (FSHR and LHB). What is known already • In our experience, the DFI, the percentage of cleaved caspase-3 and the pAKT on cumulus cells can be used as molecular markers of oocyte competence; • The polymorphic variant of LHB is characterized by an extra glycosylation signal into the β subunit. This molecular variation influences the pharmacokinetic properties of v-betaLH, showing an elevated bioactivity in vitro, but a significantly shorter half-life in circulation if compared with the wild type LH. These patients show sub-optimal ovarian response after pharmacological ovarian stimulation with r-FSH; We know that FSH receptor (FSHR) variants, Thr307/Asn680 and Ala307/Ser680, are involved in the response to ovarian stimulation. Study design, size, duration The aim of the study was to determine the apoptosis levels (in terms of percentage of DNA fragmentation and percentage of cleaved caspase-3) and the pAKT levels as potential markers of oocyte competence in the cumulus cells of individual patients with specific FSHR and LHB polymorphisms. It was a retrospective study. It was included 36 patients. The duration of the study was 12 months. Participants/materials, setting, methods Patients were normo-responder patients with a normal basic level of FSH (

Published
2016

55. RELATIONSHIP BETWEEN DNA FRAGMENTATION INDEX AND pAKT IN CUMULUS CELLS: NEW MARKERS OF OOCYTE COMPETENCE

Author

Bosco, L, Ruvolo, G, Mangiaracina, L, Picciotto, S, Roccheri, M.C, Bosco, L, Ruvolo, G, Mangiaracina, L, Picciotto, S, and Roccheri, M.C

Subjects
Apoptosis, polymorphisms of gonadotropins, cumulus cells, Settore BIO/06 - Anatomia Comparata E Citologia
Abstract

The specific LH and FSH polymorphisms could influence the growth of follicles and oocytes.Some studies have shown that certain single nucleotide polymorphisms of FSHR are associated with changes in the ovarian activity, having functional implications in human reproduction. Carriers of polymorphic variant of betaLH show sub-optimal ovarian response to the standard long GnRH-agonist down-regulation protocol, when stimulated with recombinant FSH. No studies have been designed relating the polymorphic variants of FSHR and LHB with the oocyte competence. In previous studies, we demonstrated the correlation between the apoptosis rate and the expression level of some survival pathways molecules, as pAKT, in cumulus cells, as potential markers of oocyte competence. The aim of this study is to investigate the apoptosis levels of cumulus cells pool collected from the cumulus-oocyte complex of individual patients with specific FSHR and LHB polymorphisms. Cumulus cells, obtained from 36 selected patients, were used for in situ immunocytochemistry by cleaved caspase-3, pAKT and by TUNEL assays. Genomic DNA was extracted from whole blood samples. SNPs of FSHR and LHB gene was amplified by PCR using different primers. We found the following phenotypes: - for FSHR: A/T–S/N n=18; A/A–S/S n=6; T/T–N/N n=12 - for LHB: W/W–I/I n=23; W/R– I/T n=13 Cumulus cells of patients with phenotype A/T–S/N associated with W/R–I/T (double heterozygous) showed an higher level of apoptosis in terms of DNA Fragmentation Index (DFI) and percentage of active protein caspase-3 P

Published
2016

56. Two-well quantum cascade laser optimization by non-equilibrium Green's function modelling

Author

Franckié, M., Bosco, L., Beck, M., Bonzon, C., Mavrona, E., Scalari, G., Wacker, A., Faist, J., Franckié, M., Bosco, L., Beck, M., Bonzon, C., Mavrona, E., Scalari, G., Wacker, A., and Faist, J.

Abstract

We present a two-quantum well THz intersubband laser operating up to 192 K. The structure has been optimized using non-equilibrium Green's function model. The result of this optimization was confirmed experimentally by growing, processing, and measuring a number of proposed designs. At high temperature (T > 200 K), the simulations indicate that lasing fails due to a combination of electron-electron scattering, thermal backfilling, and, most importantly, re-absorption coming from broadened states.

Published
2018

60. MITOCHONDRIAL MASS, DISTRIBUTION AND ACTIVITY DURING SEA URCHIN OOGENESIS

Author

Agnello, M., Rinaldi, A. M., Roberto Chiarelli, Bosco, L., Morici, G., Roccheri, M. C., Agnello, M, Rinaldi, A, Chiarelli, R, Bosco, L, Morici, G, Roccheri, MC, Agnello, M., Rinaldi, A., Chiarelli, R., Bosco, L., Morici, G., and Roccheri, M.

Subjects
Mitochondrial mass, sea urchin, oogenesis, MITOCHONDRIAL MASS, SEA URCHIN, OOGENESIS, Settore BIO/06 - Anatomia Comparata E Citologia
Abstract

The sea urchin egg is a favourite model for studies of the molecular biology and physiology of fertilization and early development, yet we know sparingly little of its oocytes and of mitochondria behaviour during oogenesis. The process of oogenesis in most echinoderms is asynchronous so each ovary lobe has hundreds of oocytes at all stages of development. At the beginning of oogenesis, the oocyte is about 10 µm in diameter. During the vitellogenic phase of oogenesis, the oocyte accumulate yolk proteins and grow to ten times their original size to 80 to 100 µm in sea urchins. The oocyte, arrested at the prophase of the first meiotic division, is apparent with its large nucleus, the germinal vesicle (GV), containing a prominent nucleolus. Echinoid (such as sea urchin) and Holothurian oocytes complete meiotic maturation prior to fertilization, distinct from other echinoderms and almost all others animals. As maturation progresses, it occurs the GV breaks down (GVBD). These eggs may then be stored for weeks to months within the female before they are spawned, and the proportion of eggs in the ovary increases from early to late season, as the numbers of oocytes decline [1]. Mitochondria, generally known as the powerhouses of eukaryotic cells, play a primary role in cellular energetic metabolism, homeostasis and death. These organelles, with their multicopy genome maternally inherited, are directly involved at several levels in the reproductive process since their functional status influences the quality of oocytes and contributes to the process of fertilization and embryonic development. It has been demonstrated that the number of maternal mitochondria is sufficient to support development until late stages without new synthesis of mitochondrial DNA or production of new organelles [2]. During embryogenesis mitochondrial mass does not change, whereas mitochondrial respiration increases [3]. The behaviour of these organelles during oogenesis remains at moment unclear. In the present paper we studied, by Confocal Laser Scanning Microscopy tecnologies (CLSM), the mass and distribution, the activity and the DNA content of sea urchin Paracentrotus lividus mitochondria during oogenesis, by in vivo incubating oocytes of different size with cell-permeant probes specific for mitochondria and for DNA and by immunodetection of hsp60 chaperonine, a well known mitochondrial marker. In particular the oocytes were grouped in six classes: < 10, 20/30, 40/50, 60/70, 80/90 µm, and 90 µm ovulated egg, on the base of diameters. Microscopic observations were performed capturing 2 µm thick layers of oocytes. Of the several thousands oocytes we observed, 20 for each different oogenesis stage were analyzed and processed. In order to interpret results and to draw unequivocal conclusions, we measured by IMAGE J software analysis the intensity values of fluorescent signals, as suggested in Agnello et al 2008 [4]. The mitochondria of oocytes with a diameter between 20 and 70 µm, appeared to give rise to clusters that disappear in that of 80 µm. In the oocytes between 60 and 90 µm the red fluorescence seems to be more evident around the germinal vesicle (the merge tends to red), suggesting an increasing oxidative phosphorylation activity. In the ovulated eggs, red and green fluorescence are uniformly distributed suggesting that mitochondria are dispersed in the cytoplasm. In addition the merge of green and red colours shows that the whole mitochondrial population is consuming oxygen at the same level (the resulting colours tends to yellow), figure 1. In order to calculate the total mitochondrial mass and activity we integrated the values of pixel intensities for all captured sections and used the arithmetic means to draw a statistical analysis. Results suggest a parallel rise of mitochondrial mass and activity, suggesting that the amount and activity of organelles change remarkably during oogenesis. Figure 1. shows the distribution of hsp60 protein, detected by immunofluorescence analysis (A), the mitochondrial and genomic DNA, after in vivo incubation with PicoGreen probe (B) and the merge of green and red fluorescence signal, respectively due to mitochondrial mass and activity, after in vivo incubation with Mitotraker Green and Orange (C). The size of the oocytes reported is 80 µm. Results suggest that mitochondria are actively duplicating and that mitochondrial DNA is replicating during the different oogenesis phases. It is noteworthy that around the germinal vesicle, especially in the larger oocytes, next to the germinal vesicle breakdown, the organelles are more active in oxygen consumption, probably due to the major energetic needing in this key moment of gametogenesis. [1] Wessel G.M., Voronina E., and Brooks J.M. (2004) Obtaining and handling echinoderm oocytes. In “Methods in Cell Biology”, Elsevier. Vol.74, Chapter 5, pp. 87-114. [2] Matsumoto L., Kasamatsu H., Pik´o L. and Vinograd J. (1974) Mitochondrial DNA replication in sea urchin oocytes. J. Cell Biol. 63: 146–159. [3] Morici G., Agnello M., Spagnolo F., Roccheri M.C., Di Liegro C.M. and Rinaldi A.M. (2007) Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development. Journal of Microscopy. 228: 165-173. [4] Agnello M., Morici G., Rinaldi A.M. (2008) A method for measuring mitochondrial mass and activity . Cytotechnology. 56: 145-149. Maria Carmela Roccheri: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Italy; tel: 09123897414; e-mail: maria.roccheri@unipa.it

Published
2013

63. The known knowns and known unknowns of peacekeeping data

Author

Clayton, G., Kathman, J., Beardsley, K., Gizelis, T. -I, Olsson, L., Bove, V., Ruggeri, A., Zwetsloot, R., van der Lijn, J., Smit, T., Hultman, Lisa, Dorussen, H., Diehl, P. F., Bosco, L., Goodness, C., Clayton, G., Kathman, J., Beardsley, K., Gizelis, T. -I, Olsson, L., Bove, V., Ruggeri, A., Zwetsloot, R., van der Lijn, J., Smit, T., Hultman, Lisa, Dorussen, H., Diehl, P. F., Bosco, L., and Goodness, C.

Published
2017
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65. Small size quasi-fractal microstrip antenna M1 EBG-GP

Author

Adaildo G. D'Assuncao, João Bosco L. Oliviera, Alciney Miranda Cabral, and Elder Eldervitch Carneiro de Oliveira

Subjects
Patch antenna, Engineering, Coaxial antenna, business.industry, Mechanical Engineering, Antenna factor, Condensed Matter Physics, Antenna tuner, Fractal antenna, Electronic, Optical and Magnetic Materials, Microstrip antenna, Optics, Mechanics of Materials, Electrical and Electronic Engineering, Antenna (radio), business, Monopole antenna, Computer Science::Information Theory
Abstract

A new patch antenna using the Minkowski fractal curve and Electromagnetic Bandgap structure on plane ground is proposed. The design was based on HFSS and a prototype was built and measured in order to validate the numerical results. A better impedance matching for the device was obtained using the inset-fed technique. The measured return loss for the proposed antenna at the resonant frequency is lower than �15 dB. The M1 EBG-GP antenna resonant frequency is lower than that of a corresponding rectangular inset-fed patch antenna. This results in a reduction of 35.6% for this antenna resonating at 2.45 GHz (ISM band).

Published
2012

67. A patch-array antenna single-mode low electrical dissipation continuous wave terahertz quantum cascade laser

Author

Bosco, L., Bonzon, C., Ohtani, K., Justen, M., Beck, M., Faist, J., Bosco, L., Bonzon, C., Ohtani, K., Justen, M., Beck, M., and Faist, J.

Abstract

We introduce a double metal terahertz quantum cascade laser meant for astrophysical heterodyne measurements. The laser ridge is embedded in benzocyclobutene, and the device exhibits single mode, continuous wave operation around 4.745 THz with a peak power of almost 1.8 mW at 10K and a power consumption of approximate to 1.6 W. Moreover, thanks to the integration of a top metal contact with a patch array antenna for light out-coupling the beam of the emitted light has a low-divergence single-lobe profile and an FWHM of approximate to 30 degrees. Published by AIP Publishing.

Published
2016

69. Strategies in experimental models for evaluating apoptosis

Author

Bosco, L, Agnello, M, Filosto, S, Pinsino, A, Ruvolo, G, Roccheri, MC, Bosco, L, Agnello, M, Filosto, S, Pinsino, A, Ruvolo, G, and Roccheri, MC

Subjects
sea urchin, APOPTOSIS, human, Settore BIO/06 - Anatomia Comparata E Citologia
Published
2008

70. Thermochemical parameters of complexes of di-n-propyldithiocarbamate of phosphorus-group elements

Author

Lucildes Pita M Neves, Antonio G. Souza, Claudio Airoldi, and João Bosco L de Oliveira

Subjects
Standard molar entropy, Chemistry, Enthalpy, Inorganic chemistry, Calorimetry, Condensed Matter Physics, Heat capacity, Heterolysis, Standard enthalpy of formation, Differential scanning calorimetry, Physical chemistry, Sublimation (phase transition), Physical and Theoretical Chemistry, Instrumentation
Abstract

The standard molar enthalpy of formation of crystalline di-n-propyldithiocarbamate complexes of P, As, Sb and Bi(III) was determined through reaction–solution calorimetry in acetone, at 298.15 K, giving −661.10±5.04, −305.07±5.50, −366.62±1.70 and −358.98±1.70 kJ mol−1, respectively. The corresponding standard molar enthalpies of sublimation were 127.36±4.24, 145.07±5.30, 169.45±6.08 and 185.24±5.03 kJ mol−1, respectively, estimated by means of differential scanning calorimetry. By combining the preceding values with the standard molar enthalpies of formation of the chelates in the solid phase, the standard molar enthalpies of formation in the gas phase was obtained as −533.74±7.28, −160.00±6.10, 197.17±6.31 and 173.74±5.31 kJ mol−1, respectively. From the standard molar enthalpies of formation of the gaseous chelates, the homolytic values were 217.3±3.1, 153.0±3.0, 152.5±3.0 and 139.5±2.9, and the heterolytic values were 1091.3±3.1, 969.2±3.0, 867.1±3.0 and 838.5±2.9 kJ mol−1, and the mean metal–sulphur bond-dissociation enthalpies were calculated.

Published
1999

72. The known knowns and known unknowns of peacekeeping data

Author

Clayton, G., primary, Kathman, J., additional, Beardsley, K., additional, Gizelis, T.-I, additional, Olsson, L., additional, Bove, V., additional, Ruggeri, A., additional, Zwetsloot, R., additional, van der Lijn, J., additional, Smit, T., additional, Hultman, L., additional, Dorussen, H., additional, Diehl, P.F., additional, Bosco, L., additional, and Goodness, C., additional

Published
2016
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76. The Role of Autophagy and Apoptosis During Embryo Development

Author

Agnello, M., Bosco, L., Chiarelli, R., Martino, C., Roccheri, M. C., Agnello, M., Bosco, L., Chiarelli, R., Martino, C., and Roccheri, M. C.

Abstract

Programmed cell death (PCD) and cell survival are two sides of the same coin. Autophagy and apoptosis are crucial processes during embryo development of Invertebrates and Vertebrates organisms, as they are necessary for the formation of a new organism, starting from a fertilized egg. Fertilization triggers cell remodeling from each gamete to a totipotent zygote. During embryogenesis, the cells undergo various processes, thus allowing the transformation of the embryo into an adult organism. In particular, cells require the appropriate tools to suddenly modify their morphology and protein content in order to respond to intrinsic and external stimuli. Autophagy and apoptosis are involved in cell proliferation, differentiation and morphogenesis. Programmed cell death is a key physiological mechanism that ensures the correct development and the maintenance of tissues and organs homeostasis in multicellular organisms. PCD has been classified into three types, according to the morphology that the dying cells acquire and the molecular machinery involved: PCD type I or apoptosis; PCD type II or autophagy and PCD type III or necrosis (not involved in physiological development). These different types of cell death have specific features that can be used to be identified and characterized. Apoptosis is a highly conserved, genetically-controlled process through which certain cells destroy themselves. Autophagy is an evolutionarily conserved pathway used by eukaryotes for degrading and recycling various cellular constituents, such as long-lived proteins and entire organelles, that was mainly detected in those tissues where abundant cell death is required. Both autophagy and apoptosis are induced under stress conditions as an adaptive response against stress. Usually, environmental stress cause severe effects on embryonic development. Embryos of different species, exposed to different types of physical or chemical stress, temporarily suspend their development and activate several

Published
2015
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77. A New Conductive Ink for Microstrip Antenna and Bioinspired FSS Designs on Glass and Fiberglass Substrates

Author

Marcelo David S. Mesquita, Adaildo Gomes D’Assunção, João Bosco L. Oliveira, and Yuri Max Vieira Batista

Subjects
Conductive ink, FSS, microstrip antenna, nitrocellulose, silver ink, Electrical engineering. Electronics. Nuclear engineering, TK1-9971
Abstract

Abstract This paper describes an investigation of the possibility of using a new conductive ink, insted of copper clad laminates, in the manufacturing of microstrip patch antennas on glass and fiberglass substrates and of bioinspired frequency selective surfaces (FSSs) on fiberglass substrates for wireless communication systems. The new conductive ink is developed using synthesized nitrocellulose which, in ethyl acetate solution, works as a bonding agent and carrier for the formation of a conductive film. The load used in the fabrication process is silver metallic powder. Simulation and analysis are performed using Ansoft Designer and HFSS softwares. Microstrip antenna and FSS prototypes are fabricated and measured for comparison purpose. An agreement is observed between simulation and measurements results.

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78. Tomato spotted wilt virus (TSWV) Isolates Encoding Truncated NSs Proteins Are not Transmitted by its thrips vector Frankliniella occidentalis and Are Severely Impaired in Accumulation in Adults but not in 2nd-Instar Larvae

Author

Turina M., Vallino M., Ciuffo M., Mautino G.C., Bosco L., Tavella L., and Margaria P.

Subjects
fungi, food and beverages, Transmission, Thrips, TSWV
Abstract

Tomato spotted wilt virus (TSWV) (Tospovirus, Bunyaviridae) is one of the most agronomically important viruses worldwide. The virus is transmitted between plants by insects in the genera Thrips and Frankliniella (Thysanoptera, Thripidae), in a propagative mode. Lack of reverse genetic systems hampers the functional analysis of TSWV genome, but thanks to the selective pressure of the Tsw resistance gene in pepper, we obtained a number of TSWV isolates, carrying a deletion in the NSs coding region. Such deletions caused a recovery phenotype on Capsicum spp. and Datura stramonium, linked to lack of suppression of silencing activity for the resulting truncated NSs, but in N. benthamiana some of the NSs recovery isolates accumulated to wild type level. We show that Frankliniella occidentalis, its most efficient vector, fails to transmit TSWV coding for truncated NSs protein. Quantitative RT-PCR and immuno-staining of individual insects detected the mutant virus in 2nd-instar larvae and adults, demonstrating that insects can acquire and accumulate NSs-defective mutants. Nevertheless adults carried a significantly lower viral load, showing for the first time a specific role of NSs in maintaining a high titer of virus in adult thrips. Sequence analyses of reassortant isolates revealed a strict association of lack of transmission to the mutation in the NSs-coding sequence.

Published
2013

80. Principal component analysis of the methylene chloride polar tensors

Author

Harley Paiva Martins Fo, Joao Bosco L. de Oliveira, and Roy E. Bruns

Subjects
Chemistry, Thermodynamics, Condensed Matter Physics, Biochemistry, Effective nuclear charge, Electronegativity, Dipole, Computational chemistry, Principal component analysis, Polar, Tensor, Physical and Theoretical Chemistry, Wave function, Basis set
Abstract

Principal component analysis has been applied to polar tensor data corresponding to all the possible sign alternatives of the ∂p∂Qi of CH2C12 and CD2C12. The isotopic invariance criterion and comparison of the above-mentioned values with those calculated from 6-31G(d) and 6-31++G(3d,3p) basis set wavefunctions at the Moller-Plesset 2 level permit a selection of preferred polar tensor element values for these molecules. Mean dipole derivative and effective charge values obtained from these preferred tensor element values are in excellent agreement with values estimated using electronegativity model equations considering the simplicity of the model and the errors in the measured intensities.

Published
1995

81. Oropouche fever outbreak, Manaus, Brazil, 2007-2008

Author

Mourao, Maria Paula G., Bastos, Michelle S., Gimaque, Joao Bosco L., Mota, Bruno Rafaelle, Souza, Giselle S., Grimmer, Gustavo Henrique N., Galusso, Elizabeth S., Arruda, Eurico, and Figueiredo, Luiz Tadeu M.

Subjects
Arboviruses -- Health aspects -- Distribution -- Research -- Usage, Enzyme-linked immunosorbent assay -- Usage -- Health aspects -- Research, Disease transmission -- Health aspects -- Control -- Research, Company distribution practices, Health
Abstract

To the Editor: Oropouche virus (OROV) is an arbovirus, Orthobunyavirus, transmitted among sloths, marsupials, primates, and birds by the mosquitoes Aedes serratus and Culex quinquefasciatus. Notably, this virus has adapted [...]

Published
2009
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82. Saldo de radiação diurno em dosséis de batata como função da radiação solar global

Author

HELDWEIN, A. B., MALDANER, I. C., BOSCO, L. C., TRENTIN, G., GRIMM, E. L., RADONS, S. Z., LUCAS, D. D. P., Arno Bernardo Heldwein, UFSM, Ivan Carlos Maldaner, INSTITUTO FEDERAL FARROUPILHA, Leosane Cristina Bosco, POS-GRADUANDO UFRGS, GUSTAVO TRENTIN, CPPSUL, Edenir Luis Grimm, INSTITUTO FEDERAL FARROUPILHA, Sidinei Zwick Radons, UNIVERSIDADE FEDERAL DA FRONTEIRA SUL, and Dionéia Daiane Pitol Lucas, POS-GRADUANDA UFSM.

Subjects
Radiação Solar
Published
2012

83. Disponibilidades climáticas para macieira na região de Vacaria, RS

Author

CARDOSO, L. S., BERGAMASCHI, H., BOSCO, L. C., PAULA, V. A. de, MARODIN, G. A. B., CASAMALI, B., NACHTIGALL, G. R., LOANA SILVEIRA CARDOSO, FEPAGRO, HOMERO BERGAMASCHI, UFRGS, LEOSANE CRISTINA BOSCO, UFRGS, VIVIANE AIRES DE PAULA, UFRGS, GILMAR ARDUÍNO BETTIO MARODIN, UFRGS, BRUNO CASAMALI, UFRGS, and GILMAR RIBEIRO NACHTIGALL, CNPUV.

Subjects
Fruticultura, Clima, Maçã
Abstract

O objetivo deste trabalho foi avaliar a variabilidade e as tendências temporais das principais variáveis climáticas - temperaturas máxima, mínima e média do ar, horas de frio (abaixo de 7°C), precipitação pluvial e umidade relativa do ar - no período 1983-2009 e confrontá-las com as necessidades de macieiras dos grupos Gala e Fuji, na região de Vacaria, RS. Foram utilizados dados diários de estação meteorológica da Embrapa, do período de 1983 a 2009, e comparados com as normais 1931-1960 e 1961-1990, para análise de tendência temporal. A precipitação pluvial apresentou diferenças significativas entre a média do período 1983-2009 e as normais, com aumentos de 479 e 256mm, respectivamente. Não houve diferenças significativas na temperatura do ar mensal entre os períodos analisados. A precipitação pluvial da primavera e a temperatura mínima do ar mensal apresentaram tendência significativa de aumento. O número médio de horas de frio foi de 657 de maio-agosto. O número mínimo de horas de frio delimitado no zoneamento agroclimático é atingido, na maioria dos anos, na região de Vacaria, no entanto, não significa a superação natural da dormência de gemas de macieiras. Além disso, o número de horas de frio apresentou tendência temporal de redução. Made available in DSpace on 2012-12-12T12:46:08Z (GMT). No. of bitstreams: 1 CARDODOCiRuralv42n11p19602012.pdf: 932726 bytes, checksum: 8acd1268d37a10b2de099f6ce9775a8d (MD5) Previous issue date: 2012-12-07

Published
2012

92. Expression of substance P, neurokinin 1 receptors(NK1) and neurokinin 3 receptors in the developing mouse retina and in the retina of NK1 knockout mice

Author

Catalani, Elisabetta, Dal Monte, M, Gangitano, Carlo, Lucattelli, M, Fineschi, S, Bosco, L, Bagnoli, P, Casini, G., Catalani, Elisabetta, Dal Monte, M, Gangitano, Carlo, Lucattelli, M, Fineschi, S, Bosco, L, Bagnoli, P, and Casini, G.

Abstract

—To complete a series of studies on the expression of substance P and neurokinin receptors in mammalian retinas, we investigated the occurrence of these molecules in developing mouse retinas and in retinas of mice with genetic deletion of the neurokinin 1 receptor, the preferred substance P receptor. Using semi-quantitative reverse transcription– polymerase chain reaction, we measured detectable levels of the isoform of preprotachykinin A (a substance P precursor) mRNA at postnatal day 4. Neurokinin 1 receptor and neurokinin 3 receptor mRNAs were also detected at postnatal day 4. While preprotachykinin A and neurokinin 1 receptor mRNA levels significantly increased up to eye opening (postnatal day 11), neurokinin 3 receptor mRNA levels remained constant throughout development. Substance P, neurokinin 1 receptor and neurokinin 3 receptor immunoreactivities were present at postnatal day 5. Substance P was in amacrine cells, neurokinin 1 receptor in developing amacrine and bipolar cells and neurokinin 3 receptor in OFF-type cone bipolar cells. Interestingly, a transient increase in the density of neurokinin 1 receptor immunoreactive processes was observed at eye opening in lamina 3 of the inner plexiform layer, suggesting a role of substance P and neurokinin 1 receptor in this developmental phase. However, in neurokinin 1 receptor knockout retinas, besides a significant increase of the preprotachykinin A mRNA levels, no major changes were detected: neurokinin 3 receptor mRNA levels as well as substance P and neurokinin 3 receptor immunostainings were similar to wild types. Together with previous studies, these observations indicate that there are major differences in neurokinin 1 receptor expression patterns among developing mammalian retinas. The observations in neurokinin 1 receptor knockout mice may not be applicable to rats or rabbits, and substance P and neurokinin 1 receptor may play different developmental roles in different species.

Published
2006

97. ANDROLOGY

Author

Hu, J. C. Y., primary, Seo, B. K., additional, Neri, Q. V., additional, Rozenwaks, Z., additional, Palermo, G. D., additional, Fields, T., additional, Monahan, D., additional, Rosenwaks, Z., additional, Szkodziak, P., additional, Plewka, K., additional, Wozniak, S., additional, Czuczwar, P., additional, Mroczkowski, A., additional, Lorenzo Leon, C., additional, Hernandez, J., additional, Chinea Mendez, E., additional, Concepcion Lorenzo, C., additional, Sanabria Perez, V., additional, Puopolo, M., additional, Palumbo, A., additional, Toth, B., additional, Franz, C., additional, Montag, M., additional, Boing, A., additional, Strowitzki, T., additional, Nieuwland, R., additional, Griesinger, G., additional, Schultze-Mosgau, A., additional, Cordes, T., additional, Depenbusch, M., additional, Diedrich, K., additional, Vloeberghs, V., additional, Verheyen, G., additional, Camus, M., additional, Van de Velde, H., additional, Goossens, A., additional, Tournaye, H., additional, Coppola, G., additional, Di Caprio, G., additional, Wilding, M., additional, Ferraro, P., additional, Esposito, G., additional, Di Matteo, L., additional, Dale, R., additional, Dale, B., additional, Daoud, S., additional, Auger, J., additional, Wolf, J. P., additional, Dulioust, E., additional, Lafuente, R., additional, Lopez, G., additional, Brassesco, M., additional, Hamad, M., additional, Montenarh, M., additional, Hammadeh, M., additional, Robles, F., additional, Magli, M. C., additional, Crippa, A., additional, Pescatori, E., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Zahiri, M., additional, Movahedin, M., additional, Mowla, S. J., additional, Noruzinia, M., additional, Crivello, A. M., additional, Sermondade, N., additional, Dupont, C., additional, Hafhouf, E., additional, Cedrin-Durnerin, I., additional, Poncelet, C., additional, Benzacken, B., additional, Levy, R., additional, Sifer, C., additional, Ferfouri, F., additional, Boitrelle, F., additional, Clement, P., additional, Molina Gomes, D., additional, Bailly, M., additional, Selva, J., additional, Vialard, F., additional, Yaprak, E., additional, Basar, M., additional, Guzel, E., additional, Arda, O., additional, Irez, T., additional, Norambuena, P., additional, Krenkova, P., additional, Tuettelmann, F., additional, Kliesch, S., additional, Paulasova, P., additional, Stambergova, A., additional, Macek, M., additional, Rivera, R., additional, Garrido-Gomez, T., additional, Galletero, S., additional, Meseguer, M., additional, Dominguez, F., additional, Garrido, N., additional, Mallidis, C., additional, Sanchez, V., additional, Weigeng, L., additional, Redmann, K., additional, Wistuba, J., additional, Gross, P., additional, Wuebbelling, F., additional, Fallnich, C., additional, Burger, M., additional, Schlatt, S., additional, San Celestino Carchenilla, M., additional, Pacheco Castro, A., additional, Simon Sanjurjo, P., additional, Molinero Ballesteros, A., additional, Rubio Garcia, S., additional, Garcia Velasco, J. A., additional, Macanovic, B., additional, Otasevic, V., additional, Korac, A., additional, Vucetic, M., additional, Garalejic, E., additional, Ivanovic Burmazovic, I., additional, Filipovic, M. 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G., additional, Ocal, P., additional, Sahmay, S., additional, Karahuseyinoglu, S., additional, Usta, T., additional, Repping, S., additional, Silber, S., additional, Van Wely, M., additional, Datta, A., additional, Nayini, K., additional, Eapen, A., additional, Barlow, S., additional, Lockwood, G., additional, Tavares, R., additional, Baptista, M., additional, Publicover, S. J., additional, Ramalho-Santos, J., additional, Vaamonde, D., additional, Rodriguez, I., additional, Diaz, A., additional, Darr, C., additional, Chow, V., additional, Ma, S., additional, Smith, R., additional, Jeria, F., additional, Rivera, J., additional, Gabler, F., additional, Nicolai, H., additional, Cunha, M., additional, Viana, P., additional, Goncalves, A., additional, Silva, J., additional, Oliveira, C., additional, Teixeira da Silva, J., additional, Ferraz, L., additional, Madureira, C., additional, Doria, S., additional, Sousa, M., additional, Barros, A., additional, Herrero, M. B., additional, Delbes, G., additional, Troueng, E., additional, Chan, P. T. K., additional, Vingris, L., additional, Setti, A. S., additional, Braga, D. P. A. F., additional, Figueira, R. C. S., additional, Iaconelli, A., additional, Borges, E., additional, Sargin Oruc, A., additional, Gulerman, C., additional, Zeyrek, T., additional, Yilmaz, N., additional, Tuzcuoglu, D., additional, Cicek, N., additional, Scarselli, F., additional, Terribile, M., additional, Franco, G., additional, Zavaglia, D., additional, Dente, D., additional, Zazzaro, V., additional, Riccio, T., additional, Minasi, M. G., additional, Greco, E., additional, Cejudo-Roman, A., additional, Ravina, C. G., additional, Candenas, L., additional, Gallardo-Castro, M., additional, Martin-Lozano, D., additional, Fernandez-Sanchez, M., additional, Pinto, F. M., additional, Balasuriya, A., additional, Serhal, P., additional, Doshi, A., additional, Harper, J., additional, Romany, L., additional, Fernandez, J. L., additional, Pellicer, A., additional, Ribas-Maynou, J., additional, Garcia-Peiro, A., additional, Fernandez-Encinas, A., additional, Prada, E., additional, Jorda, I., additional, Cortes, P., additional, Llagostera, M., additional, Navarro, J., additional, Benet, J., additional, Kesici, H., additional, Cayli, S., additional, Erdemir, F., additional, Karaca, Z., additional, Aslan, H., additional, Ocakli, S., additional, Tas, U., additional, Ozdemir, A. A., additional, Aktas, R. G., additional, Tok, O. E., additional, Li, S., additional, Lu, C., additional, Hwu, Y., additional, Lee, R. K., additional, Landaburu, I., additional, Gonzalvo, M. C., additional, Clavero, A., additional, Ramirez, J. P., additional, Pedrinaci, S., additional, Serrano, M., additional, Montero, L., additional, Carrillo, S., additional, Weiss, J., additional, Ortiz, A. P., additional, Castilla, J. A., additional, Sahin, O., additional, Bakircioglu, E., additional, Serdarogullari, M., additional, Bayram, A., additional, Yayla, S., additional, Ulug, U., additional, Tosun, S. B., additional, Bahceci, M., additional, Yoon, S. Y., additional, Shin, D. H., additional, Shin, T. E., additional, Park, E. A., additional, Won, H. J., additional, Kim, Y. S., additional, Lee, W. S., additional, Yoon, T. K., additional, Lee, D. R., additional, Hattori, H., additional, Nakajo, Y., additional, Kyoya, T., additional, Kuchiki, M., additional, Kanto, S., additional, Kyono, K., additional, Park, M., additional, Park, M. R., additional, Lim, E. J., additional, Choi, Y., additional, Mitra, A., additional, Bhattacharya, J., additional, Kundu, A., additional, Mukhopadhaya, D., additional, Pal, M., additional, Enciso, M., additional, Alfarawati, S., additional, Wells, D., additional, Abad, C., additional, Amengual, M. J., additional, Esmaeili, V., additional, Safiri, M., additional, Shahverdi, A. H., additional, Alizadeh, A. R., additional, Ebrahimi, B., additional, Brucculeri, A. M., additional, Ruvolo, G., additional, Giovannelli, L., additional, Schillaci, R., additional, Cittadini, E., additional, Scaravelli, G., additional, Perino, A., additional, Cortes Gallego, S., additional, Gabriel Segovia, A., additional, Nunez Calonge, R., additional, Guijarro Ponce, A., additional, Ortega Lopez, L., additional, Caballero Peregrin, P., additional, Heindryckx, B., additional, Kashir, J., additional, Jones, C., additional, Mounce, G., additional, Ramadan, W. M., additional, Lemmon, B., additional, De Sutter, P., additional, Parrington, J., additional, Turner, K., additional, Child, T., additional, McVeigh, E., additional, Coward, K., additional, Tosun, S., additional, Ciray, N., additional, Saeidi, S., additional, Shapouri, F., additional, Hoseinifar, H., additional, Sabbaghian, M., additional, Pacey, A., additional, Aflatoonian, R., additional, Bosco, L., additional, Carrillo, L., additional, Pane, A., additional, Manno, M., additional, Roccheri, M. C., additional, Selles, E., additional, Garcia-Herrero, S., additional, Martinez, J. A., additional, Munoz, M., additional, Durmaz, A., additional, Dikmen, N., additional, Gunduz, C., additional, Tavmergen Goker, E., additional, Tavmergen, E., additional, Gozuacik, D., additional, Vatansever, H. S., additional, Kara, B., additional, Calimlioglu, N., additional, Yasar, P., additional, Semerci, B., additional, Baka, M., additional, Ozbilgin, K., additional, Karabulut, A., additional, Tekin, A., additional, Sabah, B., additional, Cottin, V., additional, Kottelat, D., additional, Fellmann, M., additional, Halm, S., additional, Rosenthaler, E., additional, Kisida, T., additional, Kojima, F., additional, Sakamoto, T., additional, Makutina, V. A., additional, Balezin, S. L., additional, Rosly, O. F., additional, Slishkina, T. V., additional, Hatzi, E., additional, Lazaros, L., additional, Xita, N., additional, Makrydimas, G., additional, Sofikitis, N., additional, Kaponis, A., additional, Stefos, T., additional, Zikopoulos, K., additional, Georgiou, I., additional, Hibi, H., additional, Ohori, T., additional, Sumitomo, M., additional, Anarte, C., additional, Calvo, I., additional, Domingo, A., additional, Presilla, N., additional, Aleman, M., additional, Bou, R., additional, Guardiola, F., additional, Agirregoikoa, J. A., additional, De Pablo, J. L., additional, Barrenetxea, G., additional, Zhylkova, I., additional, Feskov, O., additional, Feskova, I., additional, Zozulina, O., additional, Somova, O., additional, Nabi, A., additional, Khalili, M. A., additional, Roudbari, F., additional, Parmegiani, L., additional, Cognigni, G. E., additional, Bernardi, S., additional, Taraborrelli, S., additional, Troilo, E., additional, Ciampaglia, W., additional, Pocognoli, P., additional, Infante, F. E., additional, Tabarelli de fatis, C., additional, Arnone, A., additional, Maccarini, A. M., additional, Filicori, M., additional, Silva, L., additional, Oliveira, J. B. A., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Cavagna, M., additional, Baruffi, R. L. R., additional, Franco, J. G., additional, Fujii, Y., additional, Endou, Y., additional, Mtoyama, H., additional, Shokri, S., additional, and Aitken, R. J., additional

Published
2012
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98. POSTER VIEWING SESSION - ANDROLOGY

Author

Dul, E. C., primary, van Ravenswaaij-Arts, C. M. A., additional, Groen, H., additional, van Echten-Arends, J., additional, Land, J. A., additional, Tyulenev, Y., additional, Naumenko, V., additional, Kurilo, L., additional, Shileiko, L., additional, Segal, A., additional, Klimova, R., additional, Kushch, A., additional, Ribas-Maynou, J., additional, Garcia-Peiro, A., additional, Abad, C., additional, Amengual, M. J., additional, Benet, J., additional, Navarro, J., additional, Colasante, A., additional, Lobascio, A. M., additional, Scarselli, F., additional, Minasi, M. G., additional, Alviggi, E., additional, Rubino, P., additional, Casciani, V., additional, Pena, R., additional, Varricchio, M. T., additional, Litwicka, K., additional, Ferrero, S., additional, Zavaglia, D., additional, Franco, G., additional, Nagy, Z. P., additional, Greco, E., additional, Romany, L., additional, Meseguer, M., additional, Garcia-Herrero, S., additional, Pellicer, A., additional, Garrido, N., additional, Dam, A., additional, Pijnenburg, A., additional, Hendriks, J. C., additional, Westphal, J. R., additional, Ramos, L., additional, Kremer, J. A. M., additional, Eertmans, F., additional, Bogaert, V., additional, Puype, B., additional, Geisler, W., additional, Clusmann, C., additional, Klopsch, I., additional, Strowitzki, T., additional, Eggert-Kruse, W., additional, Maettner, R., additional, Isachenko, E., additional, Isachenko, V., additional, Strehler, E., additional, Sterzik, K., additional, Band, G., additional, Madgar, I., additional, Brietbart, H., additional, Naor, Z., additional, Cunha-Filho, J. S., additional, Souza, C. A., additional, Krebs, V. G., additional, Santos, K. D., additional, Koff, W. J., additional, Stein, A., additional, Hammoud, I., additional, Albert, M., additional, Bergere, M., additional, Bailly, M., additional, Boitrelle, F., additional, Vialard, F., additional, Wainer, R., additional, Izard, V., additional, Selva, J., additional, Cohen - Bacrie, P., additional, Belloc, S., additional, de mouzon, J., additional, Cohen-Bacrie, M., additional, Alvarez, S., additional, Junca, A. M., additional, Dumont, M., additional, Douard, S., additional, Prisant, N., additional, Tomita, K., additional, Hashimoto, S., additional, Akamatsu, Y., additional, Satoh, M., additional, Mori, R., additional, Inoue, T., additional, Ohnishi, Y., additional, Ito, K., additional, Nakaoka, Y., additional, Morimoto, Y., additional, Smith, V. J. H., additional, Ahuja, K. K., additional, Atig, F., additional, Raffa, M., additional, Sfar, M. T., additional, Saad, A., additional, Ajina, M., additional, Braga, D. P. A. F., additional, Halpern, G., additional, Figueira, R. C. S., additional, Setti, A. S., additional, Iaconelli Jr., A., additional, Borges Jr., E., additional, Medeiros, G. S., additional, Pasqualotto, E. B., additional, Pasqualotto, F. F., additional, Nadalini, M., additional, Tarozzi, N., additional, Di Santo, M., additional, Borini, A., additional, Lopez-Fernandez, C., additional, Arroyo, F., additional, Caballero, P., additional, Nunez-Calonge, R., additional, Fernandez, J. L., additional, Gosalvez, J., additional, Gosalbez, A., additional, Cortes, S., additional, Zikopoulos, K., additional, Lazaros, L., additional, Vartholomatos, G., additional, Kaponis, A., additional, Makrydimas, G., additional, Plachouras, N., additional, Sofikitis, N., additional, Kalantaridou, S., additional, Hatzi, E., additional, Georgiou, I., additional, de Mouzon, J., additional, Amar, E., additional, Cohen-Bacrie, P., additional, Vuillaume, M. L., additional, Brugnon, F., additional, Artonne, C., additional, Janny, L., additional, Pons-Rejraji, H., additional, Fedder, J., additional, Bosco, L., additional, Ruvolo, G., additional, Bruccoleri, A. M., additional, Manno, M., additional, Roccheri, M. C., additional, Cittadini, E., additional, Bochev, I., additional, Gavrilov, P., additional, Kyurkchiev, S., additional, Shterev, A., additional, Carlomagno, G., additional, Colone, M., additional, Condorelli, R. A., additional, Stringaro, A., additional, Calogero, A. E., additional, Zakova, J., additional, Kralikova, M., additional, Crha, I., additional, Ventruba, P., additional, Melounova, J., additional, Matejovicova, M., additional, Vodova, M., additional, Lousova, E., additional, Sanchez Toledo, M., additional, Alvarez LLeo, C., additional, Garcia Garrido, C., additional, Resta Serra, M., additional, Belmonte Andujar, L. L., additional, Gonzalez de Merlo, G., additional, Pohanka, M., additional, Huser, M., additional, Amiri, I., additional, Karimi, J., additional, Goodarzi, M. T., additional, Tavilani, H., additional, Filannino, A., additional, Magli, M. C., additional, Boudjema, E., additional, Crippa, A., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Robles, F., additional, Huang, H., additional, Yao, D. J., additional, Huang, H. J., additional, Li, J. R., additional, Fan, S. K., additional, Wang, M. L., additional, Yung-Kuei, S., additional, Amer, S., additional, Mahran, A., additional, Darne, J., additional, Shaw, R., additional, Borghi, E., additional, Cetera, C., additional, Shukla, U., additional, Ogutu, D., additional, Deval, B., additional, Jansa, M., additional, Savvas, M., additional, Narvekar, N., additional, Houska, P., additional, Dackland, A. L., additional, Bjorndahl, L., additional, Kvist, U., additional, Muzii, L., additional, Barboni, B., additional, Samanta, L., additional, Kar, S., additional, Yakovenko, S. A., additional, Troshina, M. N., additional, Rutman, B. K., additional, Dyakonov, S. A., additional, Holmes, E., additional, Feijo, C., additional, Verza Junior, S., additional, Esteves, S. C., additional, Berta, C. L., additional, Caille, A. M., additional, Ghersevich, S. A., additional, Zumoffen, C., additional, Munuce, M. J., additional, San Celestino, M., additional, Agudo, D., additional, Alonso, M., additional, Sanjurjo, P., additional, Becerra, D., additional, Bronet, F., additional, Garcia-Velasco, J. A., additional, Pacheco, A., additional, Lafuente, R., additional, Lopez, G., additional, Checa, M. A., additional, Carreras, R., additional, Brassesco, M., additional, Oneta, M., additional, Savasi, V., additional, Parrilla, B., additional, Guarneri, D., additional, Laureti, A., additional, Pagano, F., additional, Cetin, I., additional, Ekwurtzel, E., additional, Morgante, G., additional, Piomboni, P., additional, Stendardi, A., additional, Serafini, F., additional, De Leo, V., additional, Focarelli, R., additional, Benkhalifa, M., additional, De Mouzon, J., additional, Entezami, F., additional, Junca, A., additional, De Mouzon, J. J., additional, Mangiarini, A., additional, Capitanio, E., additional, Paffoni, A., additional, Restelli, L., additional, Guarneri, C., additional, Scarduelli, C., additional, Ragni, G., additional, Harrison, K., additional, Irving, J., additional, Martin, N., additional, Sherrin, D., additional, Yazdani, A., additional, Almeida, C., additional, Correia, S., additional, Rocha, E., additional, Alves, A., additional, Cunha, M., additional, Ferraz, L., additional, Silva, S., additional, Sousa, M., additional, Barros, A., additional, Perdrix, A., additional, Travers, A., additional, Milazzo, J. P., additional, Clatot, F., additional, Mousset-Simeon, N., additional, Mace, B., additional, Rives, N., additional, Clarke, H. S., additional, Callow, A., additional, Saxton, D., additional, Pacey, A. A., additional, Sapir, O., additional, Oron, G., additional, Ben-Haroush, A., additional, Garor, R., additional, Feldberg, D., additional, Pinkas, H., additional, Wertheimer, A., additional, Fisch, B., additional, Palacios, E., additional, Gonzalvo, M. C., additional, Clavero, A., additional, Ramirez, J. P., additional, Rosales, A., additional, Mozas, J., additional, Castilla, J. A., additional, Mugica, J., additional, Ramon, O., additional, Valdivia, A., additional, Exposito, A., additional, Casis, L., additional, Matorras, R., additional, Bongers, R., additional, Gottardo, F., additional, Zitzmann, M., additional, Kliesch, S., additional, Cordes, T., additional, Kamischke, A., additional, Schultze-Mosgau, A., additional, Buendgen, N., additional, Diedrich, K., additional, Griesinger, G., additional, Crisol, L., additional, Aspichueta, F., additional, Hernandez, M. L., additional, Ruiz-Sanz, J. I., additional, Mendoza, R., additional, Sanchez-Tusie, A. A., additional, Bermudez, A., additional, Lopez, P., additional, Churchill, G. C., additional, Trevino, C. L., additional, Maldonado, I., additional, and Dabbah, J., additional

Published
2011
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