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56 results on '"Bonasia D. E."'

54. Tips and tricks for writing a scientific manuscript.

Author

Marmotti A, Peretti GM, Mangiavini L, de Girolamo L, Tarella C, Bonasia DE, Mattia S, Tellini A, Bellato E, Agati G, Blonna D, and Castoldi F

Subjects
Humans, Publishing, Writing
Abstract

An original scientific manuscript is the target for any researchers whose aim is to show the innovative results arising from the original intuitions that drove all their experiments. Time and patience are essential to decide how to present the data, how to conceive the tables and figures representing the main outcomes of the research, and how to read and mention the necessary references. Few basic rules may help in this difficult task. The first basic rule is: "do not follow the sequence of the paper". On the opposite, i) start writing the "Materials and Methods (or Patients and Methods when dealing with a clinical study)", ii) then write the "Results" section, iii) then, write the "Discussion" paragraph, in which the principal investigator explains the results and the innovations proposed, iv) then, write the "Introduction", which should be clear and concise. The last element to be written should be the "Abstract", which is the "interface" between the authors and the readers. The second basic rule is that any of the central chapters of the manuscript, i.e. "Materials and Methods" (MM), "Results" (R) and "Discussion" (D), should follow a methodical and sequential description of the topics in a "corresponding sequence of paragraphs". In other words, in the R and the D chapter sequence of the paragraphs should be linked to the sequence of the concepts described and discussed in the paragraphs of the MM chapter. Thus, a sequential description of concepts will be easily followed by the writers, facilitating both the authors in the organization of the data and the reader in finding a reasonable "answer" to all the aspects of the study mentioned in the MM chapter. In this article, these two rules are extensively described and several tips and tricks for each chapter are suggested to ease the composition of a scientific paper. Indeed, it may be possible to solve the complex problem of "writing a scientific paper" by means of separating it in main sections (chapters) and subsections (paragraphs) and dealing with them one by one. Naturally, this takes time and passion, but, as affirmed by Steve Jobs, "the only way to do great work is to love what you do"., (Copyright 2020 Biolife Sas. www.biolifesas.org.)

Published
2020

55. One-step cartilage repair with minced chondral fragment on a composite scaffold: an in vitro human study at low oxygen tension.

Author

Marmotti AG, Peretti GM, Mattia S, Mangiavini L, Bonasia DE, Dettoni F, Bellato E, Schwienbacher S, and Castoldi F

Abstract

Minced cartilage fragments are a viable cell source for one stage cartilage repair. However, the joint surface is a low oxygen tension microenvironment and little evidence is present in literature regarding the behaviour of cartilage fragments in this peculiar condition. The aim of the study is i) to verify if low oxygen tension could negatively influence chondrocyte outgrowth from cartilage fragments into a Hyaluronic-Acid(HA)/fibrin scaffold and ii) to evaluate its effects on the behaviour of migrating chondrocyte, compared to normoxic condition. A slight decrease in chondrocyte migration and proliferation was observed in low oxygen tension cultures. Conversely, an increase in the expression of SOX9, β-catenin, HIFs, collagen-I and II (p<0.05) in migrating chondrocytes from low oxygen tension cultures was present. Thus, a long term- exposure at low oxygen tension seems to improve the chondrocytic phenotype expression of cell outgrowing from cartilage fragments onto a HA/fibrin scaffold.

Published
2017

56. Tranexamic acid effects on cartilage and synovial tissue: an in vitro study for a possible safe intra-articular use.

Author

Marmotti A, Mattia S, Mangiavini L, Bonasia DE, Bruzzone M, Dettoni F, Rosso F, Blonna D, Rossi R, Castoldi F, and Peretti GM

Subjects
Cartilage, Articular cytology, Cells, Cultured, Chondrocytes cytology, Chondrocytes drug effects, Chondrogenesis drug effects, Humans, Synoviocytes cytology, Synoviocytes drug effects, Tranexamic Acid adverse effects, Cartilage, Articular drug effects, Tranexamic Acid pharmacology
Abstract

The possible toxic effects of intra-articular tranexamic acid (TA) are still debated. The aim of this study was to evaluate TA effects on human cartilage fragments and synovial biopsies. Explant culture of minced articular cartilage underwent prolonged TA exposure. Histological analysis, immunofluorescence and colorimetric assay for quantification of s-GAG and DNA were performed at the end term. Synoviocytes were cultured for 48h in presence of TA. Light microscopy and flow cytometry analysis were performed at the end of the exposure to TA and one week after the treatment. TA exposure did not influence i) the chondrocyte outgrowth and migration, ii) the expression of chondrogenic and proliferative markers and iii) the s-GAG/DNA ratio. TA treatment did not affect synoviocytes’ morphology and treated cells were phenotypically similar to control cells. This study demonstrated that TA does not negatively affect chondrocytes and synoviocytes cultured in vitro. Thus, our findings may be clinically relevant in order to validate the intra-articular TA administration during orthopedic procedures.

Published
2016

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