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55. Glycolaldehyde-Derived High-Molecular-Weight Advanced Glycation End-Products Induce Cardiac Dysfunction through Structural and Functional Remodeling of Cardiomyocytes.

Author

Deluyker, Dorien, Evens, Lize, Haesen, Sibren, Driesen, Ronald B., Kuster, Diederik, Verboven, Maxim, Beliën, Hanne, van der Velden, Jolanda, Lambrichts, Ivo, and Bito, Virginie

Subjects
GLYCOLALDEHYDE, HEART cells, CARDIOVASCULAR diseases, ELECTRON microscopy, MITOCHONDRIA, ELECTROPHYSIOLOGY
Abstract

Background/Aims: High-molecular-weight advanced glycation end-products (HMW-AGEs) are abundantly present in our Western diet. There is growing evidence reporting that HMWAGEs contribute to the development of cardiovascular dysfunction in vivo, next to the wellknown low-molecular-weight AGEs. The goal of our study is to assess the ultrastructure and function of cardiomyocytes after chronic exposure to HMW-AGEs. A better understanding of underlying mechanisms is essential to create new opportunities for further research on the specific role of HMW-AGEs in the development and progression of cardiovascular diseases. Methods: Adult male rats were randomly assigned to daily intraperitoneal injection for six weeks with either HMW-AGEs (20 mg/kg/day) or a control solution. Hemodynamic measurements were performed at sacrifice. Single cardiomyocytes from the left ventricle were obtained by enzymatic dissociation through retrograde perfusion of the aorta. Unloaded cell shortening, time to peak and time to 50% relaxation were measured during field stimulation and normalized to diastolic length. L-type Ca2+ current density (ICaL) and steady-state inactivation of ICaL were measured during whole-cell ruptured patch clamp. Myofilament functional properties were measured during whole-cell ruptured patch clamp. Myofilament functional properties were measured in membrane-permeabilized cardiomyocytes. Ultrastructural examination of cardiac tissue was performed using electron microscopy. Results: Rats injected with HMW-AGEs displayed in vivo cardiac dysfunction, characterized by significant changes in left ventricular peak rate pressure rise and decline accompanied with an increased heart mass. Single cardiomyocytes isolated from the left ventricle revealed concentric hypertrophy, indicated by the increase in cellular width. Unloaded fractional cell shortening was significantly reduced in cells derived from the HMW-AGEs group and was associated with slower kinetics. Peak L-type Ca2+ current density was significantly decreased in the HMW-AGEs group. L-type Ca2+ channel availability was significantly shifted towards more negative potentials after HMW-AGEs injection. The impact of HMW-AGEs on myofilament function was measured in membrane-permeabilized cardiomyocytes showing a reduction in passive force, maximal Ca2+ activated force and rate of force development. Ultrastructural examination of cardiac tissue demonstrated adverse structural remodeling in HMW-AGEs group characterized by a disruption of the cyto-architecture, a decreased mitochondrial density and altered mitochondrial function. Conclusion: Our data indicate that HMW-AGEs induce structural and functional cellular remodeling via a different working mechanism as the well-known LMW-AGEs. Results of our research open the door for new strategies targeting HMW-AGEs to improve cardiac outcome. [ABSTRACT FROM AUTHOR]

Published
2020
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58. HMW-AGEs induces Ca2+ imbalance in rat cardiomyocytes

Author

Deluyker, Dorien and Bito, Virginie

Abstract

Background. Several studies have shown that advanced glycation end products (AGEs) are associated with adverse cardiac outcome. In that context, growing evidence show that high molecular weight AGEs (HMW-AGEs) play a role as important as the well characterized low molecular weight AGEs (e.g. pentosidine and carboxymethyllysine), chronically but also possibly acutely. Objective. In this study, we investigated whether HMW-AGEs acutely alter Ca2+ homeostasis in cardiomyocytes. Methodology. Single cardiomyocytes from the left ventricle of adult male rats were obtained by enzymatic dissociation. Ionic currents were evaluated during whole cell patch-clamp in the presence or absence of HMW-AGEs (200 µg/ml) and normalized to cell capacitance. Data are expressed as mean ± SEM. Results. After 4 minutes HMW-AGEs application, peak ICaL measured at +10 mV significantly decreased (-5.2±0.6 pA/pF vs -7.8±1.1 pA/pF at baseline, ncells =10, p, Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 2 (2016): Journal of ISANH Volume 3: Special Issue for Maillard Reaction & Glycation

Published
2016
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59. Reduced mitochondrial respiration in the ischemic as well as in the remote nonischemic region in postmyocardial infarction remodeling

Author

Galan, Diogo T., primary, Bito, Virginie, additional, Claus, Piet, additional, Holemans, Patricia, additional, Abi-Char, Joëlle, additional, Nagaraju, Chandan K., additional, Dries, Eef, additional, Vermeulen, Kristel, additional, Ventura-Clapier, Renée, additional, Sipido, Karin R., additional, and Driesen, Ronald B., additional

Published
2016
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60. Assessment of left ventricular twist and untwist rate in a rat model of myocardial infarction

Author

FERFERIEVA, Vesselina, DELUYKER, Dorien, Lambrichts, Ivo, Rigo, J. M., and Bito, Virginie

Abstract

Purpose: Left ventricular (LV) twist and untwist rate (UR) are known to be strongly related to LV systolic and diastolic function, respectively. Although these parameters are emerging as important tools to evaluate LV function, yet their assessment and physiological significance in small animals remain challenging. The study was designed to investigate how twist and UR, measured by speckle tracking imaging (STI), relate to LV function and extent of fibrosis in a rat model of chronic myocardial infarction (MI). Methods: 23 Sprague-Dawley rats were subjected to LAD ligation (MI; n=14) or sham surgery (SHAM; n=9). 2D echocardiography at baseline (BL), day 1, day 3 and 2 months post-surgery was used to calculate LV dimensions, volumes (EDV, ESV) and global functional parameters. STI was applied to measure circumferential strain (Scirc), twist and UR. LV fibrosis was estimated from Sirius Red staining at day 1, day 3 and 2 months post-surgery. Results: Progressive cardiac failure evaluated during 2 months was characterized by a gradual increase in EDV and ESV respectively (0.2±0.04 mL to 0.8±0.2mL, and 0.04±0.02mL to 0.5±0.1 from BL to 2 months post-surgery, p

Published
2014

61. From Bone Marrow to Cardiac Atrial Appendage Stem Cells for Cardiac Repair: A Review

Author

Hendrikx, Marc, primary, Fanton, Yanick, additional, Willems, Leen, additional, Daniels, Annick, additional, Declercq, Jeroen, additional, Windmolders, Severina, additional, Hensen, Karen, additional, Koninckx, Remco, additional, Jamaer, Luc, additional, Dubois, Jasperina, additional, Dilling-Boer, Dagmara, additional, Vandekerkhof, Jos, additional, Hendrikx, Filip, additional, Bijnens, Eric, additional, Heuts, Nick, additional, Robic, Boris, additional, Bito, Virginie, additional, Ameloot, Marcel, additional, Steels, Paul, additional, and Rummens, Jean-Luc, additional

Published
2016
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62. Possibilities and limitations for co-transplantation of cardiac atrial appendage stem cells and mesenchymal stem cells for myocardial repair

Author

Fanton, Yanick, primary, Robic, Boris, additional, Rummens, Jean-Luc, additional, Daniëls, Annick, additional, Windmolders, Severina, additional, Willems, Leen, additional, Jamaer, Luc, additional, Dubois, Jasperina, additional, Bijnens, Eric, additional, Heuts, Nic, additional, Notelaers, Kristof, additional, Paesen, Rik, additional, Ameloot, Marcel, additional, Mees, Urbain, additional, Bito, Virginie, additional, Declercq, Jeroen, additional, Hensen, Karen, additional, Koninckx, Remco, additional, and Hendrikx, Marc, additional

Published
2016
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70. Melusin protects from cardiac rupture and improves functional remodelling after myocardial infarction

Author

UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique, UCL - (SLuc) Service de médecine interne générale, Unsöld, Bernhard, Kaul, Axel, Sbroggiò, Mauro, Schubert, Carola, Regitz-Zagrosek, Vera, Brancaccio, Mara, Damilano, Federico, Hirsch, Emilio, Van Bilsen, Marc, Munts, Chantal, Sipido, Karin, Bito, Virginie, Detre, Elke, Wagner, Nana Maria, Schäfer, Katrin, Seidler, Tim, Vogt, Johannes, Neef, Stefan, Bleckmann, Annalen, Maier, Lars S, Balligand, Jean-Luc, Bouzin, Caroline, Ventura-Clapier, Renée, Garnier, Anne, Eschenhagen, Thomas, El-Armouche, Ali, Knöll, Ralph, Tarone, Guido, Hasenfuß, Gerd, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique, UCL - (SLuc) Service de médecine interne générale, Unsöld, Bernhard, Kaul, Axel, Sbroggiò, Mauro, Schubert, Carola, Regitz-Zagrosek, Vera, Brancaccio, Mara, Damilano, Federico, Hirsch, Emilio, Van Bilsen, Marc, Munts, Chantal, Sipido, Karin, Bito, Virginie, Detre, Elke, Wagner, Nana Maria, Schäfer, Katrin, Seidler, Tim, Vogt, Johannes, Neef, Stefan, Bleckmann, Annalen, Maier, Lars S, Balligand, Jean-Luc, Bouzin, Caroline, Ventura-Clapier, Renée, Garnier, Anne, Eschenhagen, Thomas, El-Armouche, Ali, Knöll, Ralph, Tarone, Guido, and Hasenfuß, Gerd

Abstract

AIMS: Melusin is a muscle-specific chaperone protein whose expression is required for a compensatory hypertrophy response to pressure overload. Here, we evaluated the consequences of melusin overexpression in the setting of myocardial infarction (MI) using a comprehensive multicentre approach. METHODS AND RESULTS: Mice overexpressing melusin in the heart (TG) and wild-type controls (WT) were subjected to permanent LAD ligation and both the acute response (Day 3) and subsequent remodelling (2 weeks) were examined. Mortality in wild-type mice was significant between Days 3 and 7, primarily due to cardiac rupture, but melusin's overexpression strongly reduced mortality (43.2% in wild-type vs. 27.3% in melusin-TG, P = 0.005). At Day 3 after MI, a time point preceding the mortality peak, TG hearts had increased heat shock protein 70 expression, increased ERK1/2 signalling, reduced cardiomyocyte hyper-contractility and inflammatory cell infiltrates, and increased matricellular protein expression in the infarcted area. At 2 weeks after MI, melusin overexpression conferred a favourable adaptive remodelling characterized by reduced left ventricle dilatation and better preserved contractility in the presence of a comparable degree of hypertrophy. Adaptive remodelling in melusin TG mice was characterized by reduced apoptosis and fibrosis as well as increased cardiomyocyte contractility. CONCLUSIONS: Consistent with its function as a chaperone protein, melusin overexpression exerts a dual protective action following MI reducing an array of maladaptive processes. In the early phase after MI, reduced inflammation and myocyte remodelling protect against cardiac rupture. Chronically, reduced myocyte loss and matrix remodelling, with preserved myocyte contractility, confer adaptive LV remodelling.

Published
2014

72. Ruthenium red as an effective blocker of calcium and sodium currents in guinea-pig isolated ventricular heart cells

Author

Malécot, Claire O, Bito, Virginie, and Argibay, Jorge A

Subjects
Patch-Clamp Techniques, Heart Ventricles, Myocardium, Papers, Guinea Pigs, Animals, Heart, In Vitro Techniques, Calcium Channel Blockers, Ruthenium Red, Electric Stimulation, Membrane Potentials, Sodium Channel Blockers
Abstract

1. The effect of ruthenium red on calcium and sodium currents was studied in guinea-pig isolated ventricular heart cells with the whole cell patch-clamp technique. 2. Ruthenium red very efficiently blocked the L-type calcium current in a dose-dependent manner. A significant block was observed for concentrations as low as 0.3 microM. Analysis of the dose-response curve with the logistic equation indicated an EC50 of 0.8 microM, a maximum inhibition of 85% reached at 5 microM, and a coefficient of 2.37. 3. There was no shift in the voltage-dependence of the Ca current activation, nor in that of its steady-state inactivation determined with a 1 s prepulse. However, removal of Ca current inactivation at positive voltage was considerably reduced in the presence of concentrations of ruthenium red above 1 microM. A slowing of the time-course of inactivation of the Ca current was also observed. 4. At 10 microM, a concentration generally used to block the sarcoplasmic Ca release channels or the mitochondrial Ca uptake, ruthenium red blocked 26.7+/-4.3% (n=8) of the sodium current, and slowed its inactivation time-course. No effect was observed on the voltage-dependence of the current activation or inactivation. The peak sodium current was also decreased at a 10 times lower concentration by 7.6+/-2.7% (n=3). 5. Thus, at concentrations used to assess intracellular Ca movements, ruthenium red induced in heart cells a significant block of both Ca and Na channels.

Published
1998

73. Melusin protects from cardiac rupture and improves functional remodelling after myocardial infarction

Author

Unsöld, Bernhard, primary, Kaul, Axel, primary, Sbroggiò, Mauro, primary, Schubert, Carola, primary, Regitz-Zagrosek, Vera, primary, Brancaccio, Mara, primary, Damilano, Federico, primary, Hirsch, Emilio, primary, Van Bilsen, Marc, primary, Munts, Chantal, primary, Sipido, Karin, primary, Bito, Virginie, primary, Detre, Elke, primary, Wagner, Nana Maria, primary, Schäfer, Katrin, primary, Seidler, Tim, primary, Vogt, Johannes, primary, Neef, Stefan, primary, Bleckmann, Annalen, primary, Maier, Lars S., primary, Balligand, Jean Luc, primary, Bouzin, Caroline, primary, Ventura-Clapier, Renée, primary, Garnier, Anne, primary, Eschenhagen, Thomas, primary, El-Armouche, Ali, primary, Knöll, Ralph, primary, Tarone, Guido, primary, and Hasenfuß, Gerd, primary

Published
2013
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75. Combined Na + /Ca 2+ Exchanger and L-Type Calcium Channel Block as a Potential Strategy to Suppress Arrhythmias and Maintain Ventricular Function

Author

Bourgonje, Vincent J.A., primary, Vos, Marc A., additional, Ozdemir, Semir, additional, Doisne, Nicolas, additional, Acsai, Karoly, additional, Varro, Andras, additional, Sztojkov-Ivanov, Anita, additional, Zupko, Istvan, additional, Rauch, Erik, additional, Kattner, Lars, additional, Bito, Virginie, additional, Houtman, Marien, additional, van der Nagel, Roel, additional, Beekman, Jet D., additional, van Veen, Toon A.B., additional, Sipido, Karin R., additional, and Antoons, Gudrun, additional

Published
2013
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81. Transcriptome Characterization of Estrogen-Treated Human Myocardium Identifies Myosin Regulatory Light Chain Interacting Protein as a Sex-Specific Element Influencing Contractile Function

Author

Kararigas, Georgios, primary, Bito, Virginie, additional, Tinel, Hanna, additional, Becher, Eva, additional, Baczko, Istvan, additional, Knosalla, Christoph, additional, Albrecht-Küpper, Barbara, additional, Sipido, Karin R., additional, and Regitz-Zagrosek, Vera, additional

Published
2012
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86. Ultrastructural and Functional Remodeling of the Coupling Between Ca 2+ Influx and Sarcoplasmic Reticulum Ca 2+ Release in Right Atrial Myocytes From Experimental Persistent Atrial Fibrillation

Author

Lenaerts, Ilse, primary, Bito, Virginie, additional, Heinzel, Frank R., additional, Driesen, Ronald B., additional, Holemans, Patricia, additional, D'hooge, Jan, additional, Heidbüchel, Hein, additional, Sipido, Karin R., additional, and Willems, Rik, additional

Published
2009
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87. Response to Letter Regarding Article, “Ventricular Phosphodiesterase-5 Expression Is Increased in Patients With Advanced Heart Failure and Contributes to Adverse Ventricular Remodeling After Myocardial Infarction in Mice”

Author

Pokreisz, Peter, primary, Vandenwijngaert, Sara, additional, Marsboom, Glenn, additional, Gheysens, Olivier, additional, Vermeersch, Pieter, additional, Liu, Xiaoshun, additional, Gillijns, Hilde, additional, Pellens, Marijke, additional, Schoonjans, Luc, additional, Janssens, Stefan, additional, Vanhaecke, Johan, additional, Van den Bergh, An, additional, Herijgers, Paul, additional, Bito, Virginie, additional, Lenaerts, Ilse, additional, Biesmans, Liesbeth, additional, Sipido, Karin, additional, Van Lommel, Alfons, additional, Verbeken, Erik, additional, Busch, Cornelius, additional, Buys, Emmanuel, additional, and Bloch, Kenneth D., additional

Published
2009
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90. Ventricular Phosphodiesterase-5 Expression Is Increased in Patients With Advanced Heart Failure and Contributes to Adverse Ventricular Remodeling After Myocardial Infarction in Mice

Author

Pokreisz, Peter, primary, Vandenwijngaert, Sara, additional, Bito, Virginie, additional, Van den Bergh, An, additional, Lenaerts, Ilse, additional, Busch, Cornelius, additional, Marsboom, Glenn, additional, Gheysens, Olivier, additional, Vermeersch, Pieter, additional, Biesmans, Liesbeth, additional, Liu, Xiaoshun, additional, Gillijns, Hilde, additional, Pellens, Marijke, additional, Van Lommel, Alfons, additional, Buys, Emmanuel, additional, Schoonjans, Luc, additional, Vanhaecke, Johan, additional, Verbeken, Erik, additional, Sipido, Karin, additional, Herijgers, Paul, additional, Bloch, Kenneth D., additional, and Janssens, Stefan P., additional

Published
2009
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93. Remodeling of T-Tubules and Reduced Synchrony of Ca 2+ Release in Myocytes From Chronically Ischemic Myocardium

Author

Heinzel, Frank R., primary, Bito, Virginie, additional, Biesmans, Liesbeth, additional, Wu, Ming, additional, Detre, Elke, additional, von Wegner, Frederik, additional, Claus, Piet, additional, Dymarkowski, Steven, additional, Maes, Frederik, additional, Bogaert, Jan, additional, Rademakers, Frank, additional, D’hooge, Jan, additional, and Sipido, Karin, additional

Published
2008
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95. Abstract 319: Cardiomyocyte-specific Overexpression of Type 5 Phosphodiesterase Impairs Postinfarction Myocardial Function and Left Ventricular Remodeling.

Author

Pokreisz, Peter, primary, Pellens, Marijke, additional, Van Den Bergh, An, additional, Gillijns, Hilde, additional, Bito, Virginie, additional, Lenaerts, Ilse, additional, Biesmans, Liesbeth, additional, Marsboom, Glenn, additional, Vermeersch, Pieter, additional, D’hooge, Jan, additional, Sipido, Karin, additional, Herijgers, Paul, additional, Schoonjans, Luc, additional, Bloch, Kenneth D, additional, and Janssens, Stefan, additional

Published
2007
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99. Early Exercise Training Normalizes Myofilament Function and Attenuates Left Ventricular Pump Dysfunction in Mice With a Large Myocardial Infarction

Author

de Waard, Monique C., primary, van der Velden, Jolanda, additional, Bito, Virginie, additional, Ozdemir, Semir, additional, Biesmans, Liesbeth, additional, Boontje, Nicky M., additional, Dekkers, Dick H.W., additional, Schoonderwoerd, Kees, additional, Schuurbiers, Hans C.H., additional, Crom, Rini de, additional, Stienen, Ger J.M., additional, Sipido, Karin R., additional, Lamers, Jos M.J., additional, and Duncker, Dirk J., additional

Published
2007
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