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51. Assessment of dengue virus inactivation in random donor platelets using amotosalen and ultraviolet A illumination.

Author

Kumar, Ankit, Tiwari, Aseem, Kumar, Satendra, Biswas, Ashutosh, Singh, Gurpreet, Chatterjee, Kabita, Chakroborty, Sourit, and Sunil, Sujatha

Subjects
PREVENTION of infectious disease transmission, THERAPEUTIC use of ultraviolet radiation, REVERSE transcriptase polymerase chain reaction, DENGUE, HIGH performance liquid chromatography, BLOOD platelets, BLOOD collection, VIRUS inactivation, BENZOPYRANS, DESCRIPTIVE statistics, ANALYTICAL chemistry
Abstract

OBJECTIVES: The study objective was evaluation of amotosalen and ultraviolet A (UVA) illumination-based inactivation of dengue virus (DENV) in blood platelets. MATERIALS AND METHODS: Whole blood was collected from healthy donors and platelet concentrates were prepared at a tertiary care hospital in Gurugram, India. Platelet units collected from five blood group matched individuals were pooled and spiked with DENV. The spiked platelet units were subjected to amotosalen treatment followed by UVA illumination, to evaluate the efficiency of this method for viral inactivation. The treated platelet units were evaluated for the presence of infectious DENV. Amotosalen levels were quantified in the treated samples using high-performance liquid chromatography. RESULTS: The presence of replicating DENV was not observed in spiked platelet units treated with amotosalen and UVA illumination, whereas untreated units contained actively replicating DENV. Amotosalen levels were found to be in the permissible range after photochemical inactivation. CONCLUSIONS: Amotosalen/UVA pathogen inactivation treatment showed efficient inactivation of DENV in platelet components. Therefore, it seems to be a promising method for mitigating the risk of dengue transmission through transfusion of potentially contaminated platelet components in dengue-endemic countries such as India. [ABSTRACT FROM AUTHOR]

Published
2022
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52. Brain abscess in patients with chronic kidney disease: A case-based approach to management in resource-limited settings

Author

Gupta, Nitin, primary, Kumar, Rohit, additional, Banerjee, Sayantan, additional, Singh, Gagandeep, additional, Malla, Sundeep, additional, Ray, Yogiraj, additional, Ramteke, Prashant, additional, George, Netto, additional, Kodan, Parul, additional, Aggarwal, Anivita, additional, Kumar, Prabhat, additional, Jorwal, Pankaj, additional, Soneja, Manish, additional, and Biswas, Ashutosh, additional

Published
2020
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53. Zika outbreak in India in 2018

Author

Biswas, Ashutosh, primary, Kodan, Parul, primary, Gupta, Nitin, primary, Soneja, Manish, primary, Baruah, Kalpana, primary, Sharma, K K, primary, and Meena, Sheela, primary

Published
2020
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55. Hydroxychloroquine Pre-Exposure Prophylaxis for COVID-19 Among Healthcare Workers: Initial Experience from India

Author

Kadnur, Harshith B., primary, Aggarwal, Anivita, additional, Soneja, Manish, additional, Singh, Komal, additional, George, Netto, additional, Nischal, Neeraj, additional, Trilangi, Praveen, additional, Khan, Adil Rashid, additional, Desai, Devashish, additional, Mittal, Ankit, additional, Arunan, Bharathi, additional, Patidar, Diksha, additional, Gupta, Ankesh, additional, Kumar, Arvind, additional, Soni, Kapil Dev, additional, Jorwal, Pankaj, additional, Agarwal, Richa, additional, Baidya, Dalim K., additional, Mathur, Purva, additional, Bhatnagar, Sushma, additional, Trikha, Anjan, additional, Rajeshwari, S., additional, Biswas, Ashutosh, additional, Pandey, Ravindra, additional, Wig, Naveet, additional, and Guleria, Randeep, additional

Published
2020
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60. Production and characterization of human anti-V3 monoclonal antibodies from the cells of HIV-1 infected Indian donors

Author

Andrabi Raiees, Kumar Rajesh, Bala Manju, Nair Ambili, Biswas Ashutosh, Wig Naveet, Kumar Pratik, Pal Rahul, Sinha Subrata, and Luthra Kalpana

Subjects
HIV-1, Envelope glycoprotein, Third variable region, Anti-V3 monoclonal antibodies, Viral neutralization, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Analysis of human monoclonal antibodies (mAbs) developed from HIV-1 infected donors have enormously contributed to the identification of neutralization sensitive epitopes on the HIV-1 envelope glycoprotein. The third variable region (V3) is a crucial target on gp120, primarily due to its involvement in co-receptor (CXCR4 or CCR5) binding and presence of epitopes recognized by broadly neutralizing antibodies. Methods Thirty-three HIV-1 seropositive drug naive patients (18 males and 15 females) within the age range of 20–57 years (median = 33 years) were recruited in this study for mAb production. The mAbs were selected from EBV transformed cultures with conformationally constrained Cholera-toxin-B containing V3C (V3C-CTB) fusion protein. We tested the mAbs for their binding with HIV-1 derived proteins and peptides by ELISA and for neutralization against HIV-1 viruses by TZM-bl assays. Results We isolated three anti-V3 mAbs, 277, 903 and 904 from the cells of different individuals. The ELISA binding revealed a subtype-C and subtype-A specific binding of antibody 277 and 903 while mAb 904 exhibited cross reactivity also with subtype-B V3. Epitope mapping of mAbs with overlapping V3 peptides showed exclusive binding to V3 crown. The antibodies displayed high and low neutralizing activity against 2/5 tier 1 and 1/6 tier 2 viruses respectively. Overall, we observed a resistance of the tier 2 viruses to neutralization by the anti-V3 mAbs, despite the exposure of the epitopes recognized by these antibodies on two representative native viruses (Du156.12 and JRFL), suggesting that the affinity of mAb might equally be crucial for neutralization, as the epitope recognition. Conclusions Our study suggests that the anti-V3 antibodies derived from subtype-C infected Indian patients display neutralization potential against tier 1 viruses while such activity may be limited against more resistant tier 2 viruses. Defining the fine epitope specificities of these mAbs and further experimental manipulations will be helpful in identification of epitopes, unique to clade C or shared with non-clade C viruses, in context of V3 region.

Published
2012
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66. Nipah virus disease: A rare and intractable disease

Author

Banerjee, Sayantan, primary, Gupta, Nitin, additional, Kodan, Parul, additional, Mittal, Ankit, additional, Ray, Yogiraj, additional, Nischal, Neeraj, additional, Soneja, Manish, additional, Biswas, Ashutosh, additional, and Wig, Naveet, additional

Published
2019
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71. Invasive fungal infections in critically ill patients: A prospective study from a tertiary care hospital in India

Author

Durga, Chitikela Sindhura, primary, Gupta, Nitin, additional, Soneja, Manish, additional, Bhatt, Manasvini, additional, Xess, Immaculata, additional, Jorwal, Pankaj, additional, Singh, Gagandeep, additional, Ray, Animesh, additional, Nischal, Neeraj, additional, Ranjan, Piyush, additional, Biswas, Ashutosh, additional, and Wig, Naveet, additional

Published
2018
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77. Predictors of Dengue-Related Mortality and Disease Severity in a Tertiary Care Center in North India

Author

Jain, Siddharth, Mittal, Abhenil, Sharma, Surendra Kumar, Upadhyay, Ashish Datt, Pandey, Ravindra Mohan, Sinha, Sanjeev, Soneja, Manish, Biswas, Ashutosh, Jadon, Ranveer Singh, Kakade, Mahadeo B., and Dayaraj, Cecilia

Subjects
severity, predictors, outbreak, Major Article, dengue shock, mortality
Abstract

Background. There is lack of reliable predictors of disease severity and mortality in dengue. The present study was carried out to identify these predictors during the 2015 outbreak in India. Methods. This prospective observational study included confirmed adult dengue patients hospitalized between August and November 2015 in a tertiary care centre in New Delhi, India. Appropriate statistical tests were used to compare clinicolaboratory characteristics, derive predictors of severe disease and mortality, and compute a predictive score for mortality. Serotyping was done. Results. Data of 369 patients were analyzed (mean age, 30.9 years; 67% males). Of these, 198 (54%) patients had dengue fever, 125 (34%) had dengue hemorrhagic fever (grade 1 or 2), and 46 (12%) developed dengue shock syndrome (DSS). Twenty-two (6%) patients died. Late presentation to the hospital (≥5 days after onset) and dyspnea at rest were identified as independent predictors of severe disease. Age ≥24 years, dyspnea at rest and altered sensorium were identified as independent predictors of mortality. A clinical risk score was developed (12*age + 14*sensorium + 10*dyspnea), which, if ≥22, predicted mortality with a high sensitivity (81.8%) and specificity (79.2%). The predominant serotypes in Delhi (2015) were dengue virus DENV2 and DENV4. Conclusion. Age ≥24 years, dyspnea at rest, and altered sensorium were identified as independent predictors of mortality. Platelet counts did not determine outcome in dengue patients. Timely referral/access to healthcare is important. The clinical risk score for mortality prediction that was developed in this study can be used in all healthcare settings, after validation in larger cohorts.

Published
2017

95. Mortality in systemic lupus erythematosus at a teaching hospital in India: A 5-year retrospective study.

Author

Bharath, Gangadharaiah, Kumar, Prabhat, Makkar, Nayani, Singla, Paras, Soneja, Manish, Biswas, Ashutosh, and Wig, Naveet

Subjects
SYSTEMIC lupus erythematosus, TEACHING hospitals, LUPUS nephritis, ETIOLOGY of diseases, LEUKOCYTE count, STROKE, MORTALITY
Abstract

Objective: Systemic lupus erythematosus (SLE) is an autoimmune disease with an unknown etiology that can be life threatening. This study aimed to study the cause of mortality among admitted SLE patients over a period of 5 years at a teaching hospital in India. Methods: A 5-year retrospective analysis of mortality in SLE patients admitted under department of medicine of our institute was done. The presenting complaints, treatment history, clinical parameters, laboratory investigations, organ involvement, systemic lupus erythematosus disease activity index (SLEDAI), and cause of mortality were collected from the medical records on a predesigned proforma. A further analysis of two groups based on the cause of mortality was done. Results: In total, 53 death records were analyzed. Mortality in 28 SLE patients was due to high disease activity (Group I) and mortality in 25 patients was attributed due to both high disease activity and concomitant infection (Group II). Most of the patients were female (98%) and mean age of patient was 30.6 years. About 19 patients (35.8%) were diagnosed with SLE during hospital admission. Fever was the most common presenting complaint (69.8%) and lupus nephritis was the most common organ dysfunction seen (84.9%). Myocarditis was observed in 11 patients and 9 patients had cerebrovascular accident. The mean hemoglobin was lower in Group II (7.4 vs. 8.7 g/dL, P = 0.02). The median total leukocyte count was significantly higher in Group II (10,200 vs. 6600, P = 0.02). The mean serum urea and creatinine levels were also significantly higher in Group II (141.41 vs. 87.8 mg/dL, P = 0.006 and 4.7 vs. 1.7, P = 0.0001), respectively. The mean SLEDAI in Group I was 20.8 ± 8.9 and in Group II was17.7 ± 7.5. Bacterial pneumonia (17) was the most common infection, followed by tuberculosis (2) and fungal infection (2). Conclusion: Mortality among SLE patients could be due to disease flare or concomitant infection. Lung is the most common organ affected by infection in these patients. [ABSTRACT FROM AUTHOR]

Published
2019
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97. Not so sweet; severe Sweet’s syndrome presenting as SIRS and pleural effusion.

Author

Niyas Vettakkara, Kandy M., Banerjee, Sayantan, Mittal, Ankit, Goel, Pawan, Kumar, Prabhat, Baitha, Upendra, Jorwal, Pankaj, Soneja, Manish, and Biswas, Ashutosh

Subjects
SWEET'S syndrome, PLEURAL effusions, SYSTEMIC inflammatory response syndrome, SYMPTOMS, SKIN biopsy
Abstract

Acute febrile neutrophilic dermatosis (Sweet’s syndrome) is a rare inflammatory condition which presents as abrupt onset of painful erythematous plaques or nodules, often associated with fever and leucocytosis. Many extracutaneous manifestations are described in literature, but pulmonary manifestations and systemic inflammatory response syndrome (SIRS) are rare. Here we report a case of a 35‑year‑old male who presented with SIRS and pleural effusion. The presence of vesiculobullous and pustular skin lesions raised the suspicion of Sweet’s syndrome and it was confirmed by skin biopsy. Initiation of systemic glucocorticoids lead to complete resolution of symptoms. [ABSTRACT FROM AUTHOR]

Published
2018
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99. Procalcitonin as a prognostic marker for sepsis: a prospective observational study

Author

Jain, Saransh, primary, Sinha, Sanjeev, additional, Sharma, Surendra K, additional, Samantaray, J C, additional, Aggrawal, Praveen, additional, Vikram, Naval Kishore, additional, Biswas, Ashutosh, additional, Sood, Seema, additional, Goel, Manish, additional, Das, Madhuchhanda, additional, Vishnubhatla, Sreenivas, additional, and Khan, Nawaid, additional

Published
2014
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100. Cross-neutralizing activity of human anti-V3 monoclonal antibodies derived from non-B clade HIV-1 infected individuals

Author

Andrabi, Raiees, primary, Williams, Constance, additional, Wang, Xiao-Hong, additional, Li, Liuzhe, additional, Choudhary, Alok K., additional, Wig, Naveet, additional, Biswas, Ashutosh, additional, Luthra, Kalpana, additional, Nadas, Arthur, additional, Seaman, Michael S., additional, Nyambi, Phillipe, additional, Zolla-Pazner, Susan, additional, and Gorny, Miroslaw K., additional

Published
2013
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