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A blended approach encompassing problem-based learning (PBL) and structured inquiry was used in this laboratory exercise based on the congenital disease Osteogenesis imperfecta (OI), to introduce commonly used techniques in biomolecular analysis within a clinical context. During a series of PBL sessions students were presented with several scenarios involving a 2 year old child, who had experienced numerous fractures. Key learning goals related to both the theory and practical aspects of the course, covering biomolecular analysis and functional genomics, were identified in successive PBL sessions. The laboratory exercises were conducted in 3 hour blocks over six weeks, focused firstly on protein analysis, followed by nucleic acids. Students isolated collagen from normal and OI affected fibroblast cultures. Analysis by SDS-PAGE demonstrated α1 and α2 of collagen Type I chains at approximately 95 kDa and 92 kDa, respectively. Subtle differences in protein mobility between the control and OI samples were observed by some students, but most considered it inconclusive as a diagnostic tool. The nucleic acid module involved isolation of RNA from OI affected fibroblasts. The RNA was reverse transcribed and used as template to amplify a 354 bp COL1A1 fragment. Students were provided with the sequence of the OI affected COL1A1 PCR product aligned with the normal COL1A1 sequence, allowing identification of the mutation, as the substitution of Arg for Gly976 of the triple helical region. Our experience with student cohorts over several years is that presentation of this laboratory exercise within a relevant clinical context, and the opportunity for active engagement with the experimental procedures via PBL sessions, supported the learning of basic theory and practical techniques of biomolecular analysis. © 2013 by The International Union of Biochemistry and Molecular Biology 41(2):103-109, 2013 [ABSTRACT FROM AUTHOR]

In proteomics, complex mixtures of proteins are separated (usually by chromatography or electrophoresis) and identified by mass spectrometry. We have created 2DE Tandem MS, a computer program designed for use in the biochemistry, proteomics, or bioinformatics classroom. It contains two simulations-2D electrophoresis and tandem mass spectrometry. The two simulations are integrated together and are designed to teach the concept of proteome analysis of prokaryotic and eukaryotic organisms. 2DE-Tandem MS can be used as a freestanding simulation, or in conjunction with a wet lab, to introduce proteomics in the undergraduate classroom. 2DE Tandem MS is a free program available on Sourceforge at https://sourceforge.net/projects/jbf/. It was developed using Java Swing and functions in Mac OSX, Windows, and Linux, ensuring that every student sees a consistent and informative graphical user interface no matter the computer platform they choose. Java must be installed on the host computer to run 2DE Tandem MS. Example classroom exercises are provided in the Supporting Information. © 2012 by The International Union of Biochemistry and Molecular Biology [ABSTRACT FROM AUTHOR]

Concerns about public understanding of biotechnology have motivated educational initiatives to improve students' competency to make scientifically sustained decisions regarding controversial issues. Understanding students' perceptions about biotechnology is essential to determine the effectiveness of these programmes. To assess how students' perceptions are modulated, this study evaluates education and gender effects on knowledge, attitudes, interest, and importance given to biotechnology. Following a quantitative assessment approach, a questionnaire was administered to 698 high-school students engaged in three curricular formats: science students attending biology (n= 225); non-biology science students (n= 210); and non-science students (n= 263). The outcomes of this study suggest that education is more determining than gender in shaping students' perceptions and behavioural intentions, which are modulated by cognitive, affective and motivational elements. Students, particularly from non-science courses, revealed knowledge and interest limitations, but acknowledged the importance of biotechnology. Most students demonstrated positive attitudes towards different applications, except when animal manipulation was involved. Positive correlations between knowledge, attitudes, interest and importance attributed to biotechnology were identified. The implications of these findings are discussed. [ABSTRACT FROM AUTHOR]

Quantitative and qualitative studies of various aspects of the perception of biotechnology were conducted among 469 Slovenian high school students of average age 17 years. Our research aimed to explore relationships among students' pre-knowledge of molecular and human genetics, and their attitudes to four specific biotechnological applications. These applications—Bt corn, genetically modified (GM) salmon, somatic and germ line gene therapy (GT)—were investigated from the viewpoints of usefulness, moral acceptance and risk perception. In addition, patterns and quality of moral reasoning related to the biotechnological applications from the aspect of moral acceptability were examined. Clear gender differences were found regarding the relationship between our students' pre-knowledge of genetics and their attitudes to biotechnological applications. While females with a better genetics background expressed a higher risk perception in the case of GM salmon, their similarly well-educated male colleagues emphasized the risk associated with the use of germ line GT. With all four biotechnological applications, patterns of both rationalistic—deontological and teleological—and intuitive moral reasoning were identified. Students with poorer genetics pre-knowledge applied an intuitive pattern of moral reasoning more frequently than their peers with better pre-knowledge. A pattern of emotive reasoning was detected only in the case of GM salmon. A relatively low quality of students' moral reasoning, as demonstrated by their brief and small number of supporting justifications (explanations), show that there is a strong need for practising skills of argumentation about socio-scientific issues in Slovenian high schools on a much larger scale. The implications for future research and classroom applications are discussed. [ABSTRACT FROM PUBLISHER]

Genome scale experiments routinely produce large data sets that require computational analysis, yet there are few student-based labs that illustrate the design and execution of these experiments. In order for students to understand and participate in the genomic world, teaching labs must be available where students generate and analyze large data sets. We present a microarray-based gene expression analysis experiment that is tailored for undergraduate students. The methods in this article describe an expression analysis experiment that can also be applied to CGH and SNP experiments. Factors such as technical difficulty, duration, cost, and availability of materials and equipments are considered in the lab design. The microarray teaching lab is performed in two sessions. The first is an introductory wet bench exercise that allows students to master the basic technical skills. The second builds on the concepts and skills with students acquiring and analyzing the microarray data. This lab exercise familiarizes students with large-scale data experiments and introduces them to the initial analysis steps. [ABSTRACT FROM AUTHOR]

Teachers have been shown to frequently avoid addressing biotechnology topics. Aiming to understand the extent to which teachers’ scarce engagement in biotechnology teaching is influenced by their beliefs and/or by extrinsic constraints, such as practical limitations, this study evaluates biology teachers’ beliefs about biotechnology and biotechnology teaching. The findings of a survey administered to 93 secondary school teachers reveal that, in spite of holding positive beliefs about biotechnology, teachers overestimate the obstacles presented at biotechnology teaching, particularly concerning material and resource limitations. Implications of these findings for teacher training program design are discussed. [Copyright &y& Elsevier]

Agricultural biotechnology is expanding around the world, but societal acceptance remains an important issue. Michigan State University (MSU) has developed and implemented an agricultural biotechnology short course for developing countries. Four courses were taught to regional groups in 2002-2003 and eight more were taught to geographically diverse groups in 2004-2008. One half day of each course was devoted to issues related to biotechnology acceptance. The participants discussed the pro-GM and anti-GM forces in their countries. Scientists were the top ranked pro-GM force and information was the top anti-GM force. Regional differences were found between forces such as food security and economics. Possible temporal differences included a rising influence of farmers on the pro-GM side and a falling influence of environmental NGOs on the anti-GM side. [ABSTRACT FROM AUTHOR]

The purpose of this investigation was to explore students' epistemic beliefs and conceptual understanding of biotechnology. Epistemic beliefs can influence reasoning, how individuals evaluate information, and informed decision making abilities. These skills are important for an informed citizenry that will participate in debates regarding areas in science such as biotechnology. We report on an in-depth case study analysis of three undergraduate, non-science majors in a biotechnology course designed for non-biochemistry majors. We selected participants who performed above average and below average on the first in-class exam. Data from multiple sources-interviews, exams, and a concept instrument-were used to construct (a) individual profiles and (b) a cross-case analysis of our participants' conceptual development and epistemic beliefs from two different theoretical perspectives-Women's Ways of Knowing and the Reflective Judgment Model. Two independent trained researchers coded all case records independently for both theoretical perspectives, with resultant initial Cohen's kappa values above .715 (substantial agreement), and then reached consensus on the codes. Results indicate that a student with more sophisticated epistemology demonstrated greater conceptual understandings at the end of the course than a student with less sophisticated epistemology, even though the latter performed higher initially. Also a student with a less sophisticated epistemology and low initial conceptual performance does not demonstrate gains in their overall conceptual understanding. Results suggest the need for instructional interventions fostering epistemological development of learners in order to facilitate their conceptual growth. [ABSTRACT FROM AUTHOR]

Animation has great potential for improving the way people learn. A number of studies in different scientific disciplines have shown that instruction involving computer animations can facilitate the understanding of processes at the molecular level. However, using animation alone does not ensure learning. Students sometimes miss essential features when they watch only animations, mainly due to the cognitive load involved. Moreover, students seem to attribute a great deal of authority to the computer and may develop misconceptions by taking animations of abstract concepts too literally. In this study, we attempted to explore teachers' perceptions concerning the use of animations in the classroom while studying biotechnological methods, as well as the teachers' contribution to the enactment of animations in class. Thirty high-school biotechnology teachers participated in a professional development workshop, aimed at investigating how teachers plan for and support learning with animation while studying biotechnological methods in class. From that sample, two teachers agreed to participate in two case studies aimed at characterizing teachers' contribution to the enactment of animations in class while studying biotechnological methods. Our findings reveal marked teacher contribution in the following three aspects: establishing the 'hands-on' point of view, helping students deal with the cognitive load that accompanies the use of animation, and implementing constructivist aspects of knowledge construction while studying using animations. [ABSTRACT FROM AUTHOR]

Globally, science curricula have been described as outdated, and students perceive school science as lacking in relevance. Declines in senior secondary and tertiary student participation in science indicate an urgent need for change if we are to sustain future scientific research and development, and perhaps more importantly, to equip students with the knowledge and skills to make informed decisions related to scientific research. This paper argues that a good starting point would be the inclusion of more contemporary areas of science in middle school curricula. One such area with continually emerging developments is biotechnology. This paper further argues the need for research into the impact of biotechnology education that would allow students to go beyond learning about biotechnological processes and products to explore their benefits and risks through an integrated approach, where biotechnology education were extended to include subject areas beyond science, such as social sciences, health education, and English. Such an approach is important, in light of research that suggests that the general public has a limited understanding of biotechnology and that public dissemination of information is insufficient to allow individuals to make informed decisions about or to develop attitudes towards, the varied applications of biotechnology. If we are to educate students to be tomorrow's informed decision-makers, we must start by addressing their understanding of and attitudes towards emerging sciences. Further research is needed to broaden our understanding of how to achieve these goals. [ABSTRACT FROM AUTHOR]

The study of model organisms is a powerful and proven experimental strategy for understanding biological processes. This paper describes an attempt to utilize advances in yeast molecular biology to enhance student understanding by presenting a more comprehensive view of several interconnected molecular processes in the overall functioning of an organism, and introducing the concept of the inverse problem as it relates to biology. [ABSTRACT FROM AUTHOR]

Different genres of scientific articles have begun to diffuse into science curricula. Among them, adapted primary literature (APL) retains the characteristics of scientific research articles, while adapting their contents to the knowledge level of students in the 11th to 12th grades. We present three models for the teaching and learning of the opening sections of an APL article by three different biology teachers. Each of the teachers used a different model for ‘stepping into the unknown’ of the article. The analysis of the teaching approach of the opening sections of an APL in the classroom illustrates the challenges presented by the use of scientific articles for learning and instruction and the strategies that might be useful in overcoming them. By comparing those models for the APL article, we aim at facilitating the use of different genres of scientific articles in class, and promoting the design of additional teaching/learning models. [ABSTRACT FROM AUTHOR]

Graduate teaching assistants (GTAs) are gaining increasing responsibility for the instruction of undergraduate science students, yet little is known about their beliefs about science pedagogy or subsequent classroom practices. This study looked at six GTAs who were primary instructors in an introductory biology laboratory course. Teaching assistants taught a lesson about the potential social, health, and environmental impacts of genetically modified crops. Through classroom observations and in-depth interviews, the researchers examined how instructors chose to frame their lessons and what GTAs perceived as important for students to know about this particular socioscientific issue (SSI). Results showed a disconnect between the relatively mature conceptualizations of effective SSI instruction that emerged during interviews and classroom practice. [ABSTRACT FROM AUTHOR]

This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced high school biology classes. Students begin by examining a plasmid map with the goal of identifying which restriction enzymes may be used to clone a piece of foreign DNA containing a gene of interest into the vector. From the National Center for Biotechnology Initiative website, students are instructed to retrieve a protein sequence and use Expasy's Reverse Translate program to reverse translate the protein to cDNA. Students then use Integrated DNA Technologies' OligoAnalyzer to predict the complementary DNA strand and obtain DNA recognition sequences for the desired restriction enzymes from New England Biolabs' website. Students add the appropriate DNA restriction sequences to the double-stranded foreign DNA for cloning into the plasmid and infecting Escherichia coli cells. Students are introduced to computational biology tools, molecular biology terminology and the process of DNA cloning in this valuable single session, in silico experiment. This project develops students' understanding of the cloning process as a whole and contrasts with other laboratory and internship experiences in which the students may be involved in only a piece of the cloning process/techniques. Students interested in pursuing postgraduate study and research or employment in an academic biochemistry or molecular biology laboratory or industry will benefit most from this experience. BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION Vol. 39, No. 3, pp. 211-215, 2011. [ABSTRACT FROM AUTHOR]

Despite rapid growth of the biotechnology industry worldwide, a number of public concerns about the application of biotechnology and its regulation remain. In response to these concerns, greater emphasis has been placed on promoting biotechnologists' public engagement. As tertiary science degree programmes form the foundation of the biotechnology sector by providing a pipeline of university graduates entering into the profession, it has been proposed that formal science communication training be introduced at this early stage of career development. The aim of the present study was to examine the views of biotechnology students towards science communication and science communication training. Using an Australian biotechnology degree programme as a case study, 69 undergraduates from all three years of the programme were administered a questionnaire that asked them to rank the importance of 12 components of a biotechnology curriculum, including two science communication items. The results were compared to the responses of 274 students enrolled in other science programmes. Additional questions were provided to the second year biotechnology undergraduates and semi-structured interviews were undertaken with 13 of these students to further examine their views of this area. The results of this study suggest that the biotechnology students surveyed do not value communication with non-scientists nor science communication training. The implications of these findings for the reform of undergraduate biotechnology courses yet to integrate science communication training into their science curriculum are discussed. [ABSTRACT FROM AUTHOR]

Public concerns about biotechnology have resulted in greater attention being paid to the mechanisms by which biotechnology is communicated with non-scientists, including the provision of science communication training. As undergraduate and postgraduate courses form the foundation of the biotechnology sector by providing a pipeline of university graduates entering into the profession, it has been proposed that formal science communication training be introduced at this early stage of career development. Using an Australian biotechnology degree course as a case study, this paper examines science communication training within this course and the views of past and present students towards this training. Interviews were undertaken with 22 stakeholders in the case, including undergraduate lecturers (who also supervise postgraduate research students), doctoral candidates and biotechnologists recently graduated from the course. Few of the students felt the course provided them with any form of science communication training, let alone training in how to engage non-scientists. Many were unaware of the training available to them and few of the lecturers were able to identify where communication skills are taught within the course. A previous study of this case has also shown that biotechnology undergraduates taking this course do not value communication with non-scientists. Clearly, the current state of science communication training for these students needs to be improved if they are to enter the biotechnology workforce as able civic scientists. The findings of this study may be useful for other university biotechnology courses which have yet to integrate science communication training into their curriculum. [ABSTRACT FROM AUTHOR]

As biotechnology-related scientific advances, such as stem cell research (SCR), are increasingly permeating the popular media, it has become ever more important to understand students' ideas about this issue. Very few studies have investigated learners' ideas about biotechnology. Our study was designed to understand the types of alternative conceptions students hold concerning SCR. The qualitative research design allowed us to examine college students' understandings about stem cells and SCR. More specifically, we addressed the following questions: How can alternative conceptions about stem cell topics be categorized? What types of alternative conceptions are most common? Participants included 132 students enrolled in a biotechnology course that focused on the scientific background of biotechnology applications relevant to citizens. In this study, we used an inductive approach to develop a taxonomy of alternative ideas about SCR by analyzing student responses to multiple open-ended data sources. We identified five categories of conceptions: alternative conceptions about what, alternative conceptions about how, alternative conceptions about medical potential, terminology confusion, and political and legal alternative conceptions. In order to improve instruction, it is important to understand students' ideas when entering the classroom. Our findings highlight a need to teach how science can be applied to societal issues and improve science literacy and citizenship. [ABSTRACT FROM AUTHOR]

A key goal of molecular/cell biology/biotechnology is to identify essential genes in virtually every physiological process to uncover basic mechanisms of cell function and to establish potential targets of drug therapy combating human disease. This article describes a semester-long, project-oriented molecular/ cellular/biotechnology laboratory providing students, within a framework of bone cell biology, with a modern approach to gene discovery. Students are introduced to the topics of bone cells, bone synthesis, bone resorption, and osteoporosis. They then review the theory of microchip gene arrays, and study microchip array data generated during the differentiation of bone-resorbing osteoclasts in vitro. The class selects genes whose expression increases during osteoclastogenesis, and researches them in small groups using web-based bioinformatics tools. Students then go to a biotechnology company website to find and order small inhibitory RNA5 (siRNAs) designed to "knockdown" expression of the gene of interest. Students then learn to transfect these siRNAs into osteoclasts, stimulate the cells to differentiate, assay osteoclast differentiation in vitro, and measure specific gene expression using real-time PCR and immunoblotting. Specific siRNA knockdown resulting in a decrease in osteoclastogenesis is indicative of a gene's physiological relevance. The results are analyzed statistically and presented to the class in groups. In the past 2 years, students identified several genes essential for optimal osteoclast differentiation, including Myold. The students hypothesize that the myold protein functions in osteoclasts to deliver important proteins to the cell surface via vesicular transport along microfilaments. Student response to the new course was overwhelmingly positive. [ABSTRACT FROM AUTHOR]

The implementation of good laboratory practices (GLPs) is recognized by the biotechnology and pharmaceutical industries as being critical for ensuring the international acceptability of products. However, as universities and colleges (and research organizations) do not necessarily work under similar constraints, actual laboratory practices vary widely, and students (or employees) are not required to have any exposure to GLP standards and stipulations. This essay makes a case for formally introducing principal investigators, students, and other laboratory personnel to the GLP regime. Many of these practices, while not absolutely required in academic laboratories, could nevertheless facilitate smooth and efficient running of the laboratory and also equip students with the knowledge and experience necessary for a facile transition to an industrial setting. [ABSTRACT FROM AUTHOR]

The importance of biotechnology education at the high-school level has been recognized in a number of international curriculum frameworks around the world. One of the most problematic issues in learning biotechnology has been found to be the biotechnological methods involved. Here, we examine the unique contribution of an animation of the polymerase chain reaction (PCR) in promoting conceptual learning of the biotechnological method among 12th-grade biology majors. All of the students learned about the PCR using still images ( n = 83) or the animation ( n = 90). A significant advantage to the animation treatment was identified following learning. Students’ prior content knowledge was found to be an important factor for students who learned PCR using still images, serving as an obstacle to learning the PCR method in the case of low prior knowledge. Through analysing students’ discourse, using the framework of the conceptual status analysis, we found that students who learned about PCR using still images faced difficulties in understanding some mechanistic aspects of the method. On the other hand, using the animation gave the students an advantage in understanding those aspects. [ABSTRACT FROM AUTHOR]

Of concern is an international trend of students’ increasing reluctance to choose science courses in both their final years of secondary school and tertiary levels of education. Research into the phenomenon indicates an influencing factor to be the ‘uninteresting curriculum’ (OECD ) of school science. This paper presents an exploration of what biotechnology key ideas students and teachers consider to be interesting. A survey was constructed and completed by 500 Australian students and their 35 teachers. Interviews were conducted with a sample of students and teachers. The Chi-square statistics revealed a significant difference between the student and teacher survey responses in four of the six a priori factors. A rank ordering of the key ideas, based on whole group mean scores, indicates only a small overlap in modern biotechnology key ideas of interest to both the students and teachers. The results suggest the key ideas teachers are interested in and incorporate into their curriculum, are not the key ideas students are interested in learning about. This mismatch is particularly prevalent and problematic in situations where curriculum choice is available within a mandated framework or syllabus, which is the case for these teachers and students. The study also found students withdrawing from biology courses in post compulsory settings due to lack of interest and perceived lack of relevance of the course. [ABSTRACT FROM AUTHOR]

It was proposed that an analysis of the questions students anticipate asking, and ask, could provide information about an enculturation encounter between Year 13 biology students and scientists working in a biomedical-clinical research unit. As part of a day-long intervention at this research institute, small groups of students (10-15) met with scientists (two) for a 15-minute discussion period. Pre- and post-questionnaires from 398 students provided data on intended, and judged best questions that were categorised and analysed into five categories: nature of science, science information, citizen decisions, personal, and no response/other. Chi Square analysis showed that students' areas of interest shifted to a personal perspective as a result of the intervention. Twenty students were interviewed who provided explanations for their questions. Analysis of their responses showed students were: developing an understanding of scientific practice as a journey, making identity links, using the personal as a knowledge bridge, acknowledging a commonality of values, and demonstrating that such an enculturation can be a transformative experience. These students engaged with a community of scientists at a physical, cognitive, and personal level. Physically they engaged with the practice of science in the laboratory, cognitively they were able to develop an understanding about how science knowledge was developed, and personally they were able to identify with science and scientists. The shift in students' questions showed that the intervention influenced their views on science and scientists to a broader understanding of scientific literacy. [ABSTRACT FROM AUTHOR]

Adapted primary literature (APL) is a novel text genre that retains the authentic characteristics of primary literature. Learning through APL represents an educational intervention with an authentic scientific context. In this case study, we analyzed the 80-min discourse developed during the enactment of an article from an APL-based curriculum in biotechnology in one class, and examined epistemic practices used by students during their meaning-making of the Results and Discussion sections of the article. Specifically, we examined coordination practices, by which students connected elements belonging to different epistemic status or context (theory, data, experimental stages, biotechnological applications and text). The application of coordination practices was identified more than 70 times during the lesson. In the context of the Results section, the students displayed research-oriented coordination practices, which were frequently associated with claims of comprehension difficulty. In the context of the Discussion section, students displayed text-oriented coordination practices, associated with analysis of the text characteristics. We are suggesting that the research-oriented coordination practices and some of the text-oriented ones enabled the emergence of authentic scientific practices and learning by inquiry. Another type of text-oriented coordination practice enabled reflection on scientists’ experimental processes, enabling learning science as inquiry. The enactment model of APL used here allowed for both the emergence of the two dimensions of inquiry learning and the promotion of scientific literacy in the fundamental and derived senses. [ABSTRACT FROM AUTHOR]

Immunology is gaining prominence both in the media as well as on the Advanced Placement (AP) exam in Biology. One of the challenges of teaching modern biological topics such as immunology and biochemistry in the high-school setting is the increased reliance on expensive technology in the research world. To begin to bridge this widening gap, we devised an experiment using a novel macroscale enzyme-linked immunosorbent assay that is suitable for AP-level high-school biology classrooms as well as entry-level collegiate laboratories. This novel method does not require a plate reader for data analysis, but instead relies on more common and inexpensive equipment such as a clinical test tube centrifuge and a simple test tube spectrophotometer. The experimental plan focuses on students measuring antibody concentrations in "unknown" samples and includes the collection and analysis of a standard curve using reagents prepared by the instructor. Students will be introduced to enzyme action, quantitative laboratory technique, antibodies, and the immune system, with the overall goal being to explore and highlight the inherent connections within the fields of biochemistry and immunology. [ABSTRACT FROM AUTHOR]

This new course in biotechnology for upper division undergraduates provides a comprehensive overview of the process of drug discovery that is relevant to biopharmaceutical industry. The laboratory exercises train students in both cell-free and cell-based assays. Oral presentations by the students delve into recent progress in drug discovery. Combination of lectures, hands-on experiments, oral presentations, and accurate recording of laboratory data provides students a thorough training in biotechnology that better prepares them for the job market. [ABSTRACT FROM AUTHOR]

Mock outbreaks of infectious disease offer the ability to introduce principles of food microbiology, ecology, and epidemiology to undergraduate students using an inquiry driven process. Students were presented with an epidemiological case study detailing patient history, clinical presentation, and foods recently consumed. The students then had to form hypotheses about the causative agents and attempt to identify the vehicle of transmission from foods their patient consumed. Students applied biochemical testing protocols to explore bacterial metabolism that are used to identify pathogens. After biochemically identifying their case's causative agent, the students were introduced to rapid methods that are used by health departments. Students extracted DNA, performed PCR reactions to amplify the 16s rDNA gene a9d identified it by comparing the PCR product sequence to a database using BLAST. Rapid methods, which do not employ time-consuming culturing methods, are increasingly being used; this exercise ensures that students have the technical skills necessary to compete in an increasingly technological global market. [ABSTRACT FROM AUTHOR]

This article discusses the challenges that bioinformatics education is facing and describes a bioinformatics course that is successfully taught at the California State Polytechnic University, Pomona, to the fourth year undergraduate students in biological sciences, chemistry, and computer science. Information on lecture and computer practice topics, free for academic use software and web links required for the laboratory exercises and student surveys for two instances of the course, is presented. This course emphasizes hands-on experience and focuses on developing practical skills while providing a solid knowledge base for critically applying these skills. It is designed in blocks of 1-hour lecture followed by 2 hours of computer laboratory exercises, both covering the same general topic, for a total of 30 hours of lecture and 60 hours of computer practice. The heavy computational aspect of this course was designed to use a single multiprocessor computer server running Linux, accessible from laptops through Virtual Network Computing sessions. The laptops can be either provided by the institution or owned by the individual students. This configuration avoids the need to install and maintain bioinformatics software on each laptop. Only a single installation is required for most bioinformatics software on the Linux server. The content of this course and its software/hardware configuration are well suited for institutions with no dedicated computer laboratory. This author believes that the same model can be successfully implemented in other institutions, especially those who do not have a strong instructional computer technology support such as community colleges and small universities. [ABSTRACT FROM AUTHOR]

One of the challenges of the Biotechnology industry is keeping up to date with the rapid pace of change and that much of the information, which students learn in their undergraduate studies, will be out of date in a few years. It is therefore crucial that Biotechnology students have the skills to access the relevant information for their studies and critically evaluate the vast volume of information and its sources. By developing information literacy skills, which are part of lifelong learning, Biotechnology graduates are better prepared for their careers. Students also need to understand the issues related to the use of information such as social, political, ethical, and legal implications. This paper will outline the embedding of information literacy skills within the Biotechnology degree at the University of South Australia. Examples of specific activities and their link to assessment will be discussed. [ABSTRACT FROM AUTHOR]

Molecular modeling is pervasive in the pharmaceutical industry that employs many of our students from Biology, Chemistry and the interdisciplinary majors. To expose our students to this important aspect of their education we have incorporated a set of tutorials in our Biochemistry class. The present article describes one of our tutorials where undergraduates use modeling experiments to explore the structure of an estrogen receptor. We have employed the Molecular Operating Environment, a powerful molecular visualization software, which can be implemented on a variety of operating platforms. This tutorial rein- forces the concepts of ligand binding, hydrophobicity, hydrogen bonding, and the properties of side chains and secondary structure taught in a general biochemistry class utilizing a protein that has importance in human biology. [ABSTRACT FROM AUTHOR]

This study examined university students' knowledge of and attitudes (n = 378) toward biotechnology in Slovakia, a conservative country where the distribution of genetically engineered products are banned by law. We found a significant positive correlation between attitudes and the level of knowledge; however, although students enrolled in biology courses have better knowledge of biotechnology, their attitudes toward genetic engineering were similar than those of students who do not study biology. Females showed poorer knowledge and lower acceptance of genetically engineered products than did males. Overall, Slovakian students have poor knowledge and numerous misunderstandings about what genetic engineering means, which suggests that science curriculum with respect to this topic should be greatly re-evaluated and teaching strategies should be improved accordingly. [ABSTRACT FROM AUTHOR]

The products of modern biotechnology processes such as genetic engineering, DNA testing and cloning will increasingly impact on society. It is essential that young people have a well-developed scientific understanding of biotechnology and associated processes so that they are able to contribute to public debate and make informed personal decisions. The aim of this study was to examine the development of understandings and attitudes about biotechnology processes as students progress through high school. In a cross-sectional case study, data was obtained from student interviews and written surveys of students aged 12 to 17 years. The results indicate that students' ability to provide a generally accepted definition and examples of biotechnology, cloning and genetically modified foods was relatively poor amongst 12–13 year old students but improved in older students. Most students approved of the use of biotechnology processes involving micro-organisms, plants and humans and disapproved of the use of animals. Overall, 12–13 year old students' attitudes were less favourable than older students regardless of the context. An awareness of the development and range of students' understandings and attitudes may lead to a more appropriate use of biotechnology curriculum materials and thus improved biotechnology education in schools. [ABSTRACT FROM AUTHOR]

Biotechnology is one of the active domains in the NSF funded Engineering Research Center VaNTH ( Vanderbilt, Northwestern, University of Texas, and Harvard/MIT) where educational modules have been developed. These modules cover a collection of challenges designed around bioreactors, mass and momentum transfer issues, microbial kinetics, and downstream processing, which are among core biotechnology topics. The aim of this study was to design educational modules centered on challenge-based education and to implement them in classroom settings. This paper focuses on the design and implementation of such educational modules and provides an overview of the challenges and learning activities that were developed for three specific topics that have been implemented at Northwestern and Vanderbilt Universities. [ABSTRACT FROM AUTHOR]

Research in biotechnology is rapidly advancing; everyday, new and exciting discoveries are made. With this new technology there are also many safety and ethical questions, though, as well as the need for education. Alternative teaching methods may help to increase students' understanding of difficult concepts in all aspects of schooling, including mathematics, science, genetics, and biotechnology. The Genomic Analogy Model for Educators (GAME) is a teaching tool currently under development, made up of three different pieces: (i) a CD-Rom, (ii) a website and (iii) laboratory exercises. The GAME model uses simple analogies of easily understandable concepts to explain the technical and scientific aspects of modern genomics; the first module is the Lego® Analogy Model (LAM) which focuses on DNA sequencing using the Sanger method and electrophoresis. The purpose of this study was to evaluate the effectiveness of the GAME model on high school students. In addition, the short term effect of the GAME model on high school students' attitudes about biotechnology was also measured. Results showed a positive change in students' post-test scores after participating in GAME which indicates the effectiveness of this new tool for biotechnology education. [ABSTRACT FROM AUTHOR]

From the middle of the 20th century, Chinese scientists have been actively involved in biotechnology. However, biotechnology education in China is a relatively recent phenomenon. This subject has not been addressed at the undergraduate level in a serious way until recently. In the last decade, biotechnology education developed rapidly and reached a new level in Chinese universities. The Chinese scientific establishment is very much aware of the importance of biotechnology and has identified this subject as one of the priority areas. Some universities are taking positive steps toward enhancing biotechnology education. This article focuses on the emergence, as well as the problems and prospects, of biotechnology education in China. [ABSTRACT FROM AUTHOR]

Four mini-practicals are described in which the effects of temperature and pH on phytase activity are assessed, as well as the enzyme's thermostability and the effect upon stability of simulated digestive tract conditions. Phytase is routinely incorporated into monogastric animal feed to ameliorate the negative nutritional and environmental consequences of its substrate, dietary phytic acid. In addition to illustrating selected basic concepts in enzymology, the combined experiments allow the students to determine the suitability of the test phytase for inclusion in animal feed. As such the practical mimics an applied research project and is particularly suited to biotechnology students undertaking courses in basic biochemistry. Students may be segregated into groups of 4, with each team member charged with undertaking one of the mini-experiments. In this way students are given individual responsibility and learn to work as part of an integrated research grouping. [ABSTRACT FROM AUTHOR]

We report the development of a simplified procedure for restriction fragment length polymorphism (RFLP) analysis of mushrooms. We have adapted standard molecular techniques to be amenable to an undergraduate laboratory setting in order to allow students to explore basic questions about fungal diversity and relatedness among mushroom species. The streamlined protocols allowed students to practice important molecular techniques within the context of self-designed investigative projects. This laboratory experience provided opportunities for students to practice strategies for examining molecular diversity among species. [ABSTRACT FROM AUTHOR]

Courses in introductory biochemistry invariably encompass basic principles of enzymology, with reinforcement of lecture-based material in appropriate laboratory practicals. Students undertaking practical classes are more enthusiastic, and generally display improved performance, when the specific experiments undertaken show direct relevance to their core degree area. Herein is described an enzyme-based practical of particular relevance to students undertaking primary degrees in biotechnology, environmental science, nutrition, as well as animal and veterinary science. The practical entails assay of phytase, an enzyme that is now routinely included in monogastric animal feed in order to ameliorate the negative nutritional and environmental consequences of its substrate, dietary phytic acid. The practical is technically straightforward, requiring relatively basic laboratory equipment and reagents that are both easily obtained and relatively inexpensive. To be an effective digestive supplement, the enzyme must display a combination of physicochemical properties, which provide sufficient scope for an interesting and multifaceted postlaboratory discussion. [ABSTRACT FROM AUTHOR]

Commercial-scale research translation has been muted.

Terms to be familiar with before you start to solve the test: 3'- and 5'-ends of DNA fragments, plasmids, restriction endonucleases, agarose gel electrophoresis, linear and circular DNA, superhelical DNA, blunt and sticky ends, DNA ligase, nuclease, [γ-32P] ATP, and radioactive labeling [ABSTRACT FROM AUTHOR]

With the ongoing laboratory restrictions, it is often challenging for bioscience students to make satisfactory progress in their projects. A long-standing practice in multi-disciplinary research is to use computational and theoretical method to corroborate with experiment findings. In line with the lack of opportunity to access laboratory instruments, the pandemic situation is a win-win scenario for scholars to focus on computational methods. This communication outline some of the standalone tools and webservers that bioscience students can successfully learn and adopt to obtain in-depth insights into biochemistry, biophysics, biotechnology, and bioengineering research work., (© 2021. The Author(s).)

This study presents an evaluation of integrating virtual laboratory simulations in assessment design of a biotechnology course at Taylor's University in Malaysia before, during and post-COVID recovery phases. The purpose was to investigate how virtual laboratory simulations were integrated as part of the assessments of a practical-embedded course-the aim being to evaluate students' acceptance and perception of using virtual simulation. A total of 46 students, across three different study cohorts (August 2019, March 2020, and August 2020) were evaluated different educational aspects of using virtual laboratory cases in a 4-week course within Animal Biotechnology. Overall, students regarded virtual laboratory simulation useful as part of their learning, and there is a significant increase in the level of acceptance before, during and post-COVID recovery phases. The study showed that across the different study cohorts, students perceived their confidence level in laboratory skills have been enhanced and that they can apply the skills in real-life situation. Interestingly, students (March and August 2020 cohort) who have not been exposed to the related laboratory session still perceived that the simulated activity provides clear explanation and realistic experience. Furthermore, it had been highlighted across the study cohorts that the quiz questions helped to enhance their understanding on the underlying principles of the laboratory techniques. The overall conclusion of this study was that structured simulation-based activities which provide clear instructions and explanation would support significant improvements in students learning., (© 2021 International Union of Biochemistry and Molecular Biology.)

A survey was carried out to investigate the knowledge, perception and interest of secondary school students about biotechnology. A total of 334 questionnaires were distributed to students in Senior Secondary 3 classes from eight secondary schools within Calabar metropolis. Data was collected and analyzed using SPSS version 7.5. Results revealed 105 (34.21%) of students had limited knowledge of medical biotechnology, genetic engineering and genetically modified products. 91 (30.03%) of the students agreed that biotechnology is the use of living organisms to produce goods and services while 102 (33.41%) accepted that biotechnology is a new technology based on biology 62 (20.39%) were willing to embrace the applications of biotechnology. 152 (50.03%) do not agree that biotech will improve services for mankind; 90 (29.51) had no idea about the applications of biotechnology; 31 (10.24%) students disagreed that cloning results in perfectly identical individuals while the highest positive responses; 92 (30.31%) was recorded for questions regarding in vitro fertilization. The students also showed a very low interest 76 (25%) in pursuing biotechnology as a degree in the University. Generally students' knowledge, perception, and interest in Biotechnology were low among Secondary School students in Calabar. There is a need to immediately increase enlightenment and emphasis on the applications of biotechnology among Secondary school students to enable them appreciate the benefits of biotechnology., (© 2021 International Union of Biochemistry and Molecular Biology.)

Biotechnology is the manipulation and application of the whole or a part of a living biological system such as microorganisms, plants and animals for the production of goods and services for the benefits of mankind and society at large. Various aspect of biotechnology has been identified based on their applications in medicine, industry and agriculture. Medical biotechnology involves medical application of biotechnology for disease diagnosis, prevention and treatment. One of the major factors to be considered in delivery of quality health services in developing countries like Nigeria is the rapid diagnosis of diseases and infections. This will in turn help in subsequent rapid treatment and prevention. Molecular diagnostic techniques are vital tools required for detection of diseases and infections. Harnessing inherent potentials of medical biotechnology will help in early detection of diseases such as Acquired Immunodeficiency Syndrome (AIDS), Sexually Transmitted Infections (STI), communicable and infectious diseases in developing countries. It will also help in reducing the work load of diagnostic laboratories. This paper therefore highlights significance of harnessing medical biotechnology in enhancing delivery of quality health service in Nigeria. [ABSTRACT FROM AUTHOR]

While science students are well prepared for careers in biotechnology and pharmaceutical sciences in terms of technical expertise and critical thinking, they rarely have an opportunity to practice the due diligence required for success in industry in their coursework. This includes framing their expertise as solutions to challenges a company may be experiencing, an important skill for the interview process. As most academics have not applied for positions in industry, they may feel ill equipped to help students practice the important skills of framing their expertise within company goals and to discuss the business and financial concepts relevant to careers in scientific industry. Here, we describe an educational activity first developed by a leader in the biotech/pharmaceutical industry that was modified and given educational context by an academic in a class of upper-level undergraduate and graduate students. In this guided inquiry activity, students were instructed to select a start-up company in their field-ideally one to which they intended to apply for a job. Students were empowered by scaffolded hands-on exercises to research the company's scientific focus and finances, and to frame how their expertise could help companies achieve stated goals. Students compiled and delivered their research as an in-class presentation., (© 2021 International Union of Biochemistry and Molecular Biology.)

Synthetic biology and especially xenobiology, as emerging new fields of science, have reached an intellectual and experimental maturity that makes them suitable for integration into the university curricula of chemical and biological disciplines. Novel scientific fields that include laboratory work are perfect playgrounds for developing highly motivating research-based teaching modules. We believe that research-based learning enriched by digital tools is the best approach for teaching new emerging essentials of academic education. This is especially true when the scientific field as such is still not canonized with text books and best-practice examples. Our experience shows that iGEM/BIOMOD competitions represent an excellent basis for designing research-based courses in xenobiology. Therefore, we present a report on "iGEM-Synthetic Biology" offered at the Technische Universität Berlin as an example., (© 2020 Wiley-VCH GmbH.)

Engagement in undergraduate research experiences (UREs) positively impacts student skill development, scientific identity, and retention in STEM. Incorporating UREs into 2-year programs would greatly benefit the diverse, nontraditional student populations enrolled at community colleges. This article describes the infusion of the Bioscience/Biotechnology program at Delaware Technical Community College with course-based and mentored research experiences that could serve as a model for other institutions. Studies done with the Office of Institutional Research revealed a concurrent increase in enrollment and graduation rates. Retrospective interviews with graduates from the program highlight the critical influence of research, the mentor/student relationship, and a sense of community on the development of transferable skills, self-efficacy, and subsequent successes in pursuing higher education and employment.

To achieve ambitious 21st-century goals and deal with high-level complexity, a bio-based economy is required to cross the boundaries of a single sector and integrate tools, language and knowledge drawn from different disciplines and sub-disciplines. The present contribution highlights how life scientists, social scientists, policymakers and industrial stakeholders should work together to make this technological reversal real and feasible. Importantly, going beyond theoretical and methodological integration, the paper underlines the necessity of developing a new and more flexible educational framework that might facilitate interdisciplinary combination. Specifically, the experience of the summer school "Towards a bio-based economy: science, innovation, economics, education" organized by the University of Milano Bicocca in collaboration with Chalmers University is described. The results reveal the need for high-level education programs likely to promote and guide society towards bio-based innovation., (Copyright © 2020 Elsevier B.V. All rights reserved.)

CRISPR-cas technology is being incorporated into undergraduate biology curriculum through lab experiences to immerse students in modern technology that is rapidly changing the landscape of science, medicine and agriculture. We developed and implemented an educational module that introduces students to CRISPR-cas technology in a Genetic course and an Advanced Genetics course. Our primary teaching objective was to immerse students in the design, strategy, conceptual modeling, and application of CRISPR-cas technology using the current research claim of the modification of the CCR5 gene in twin girls. This also allowed us to engage students in an open conversation about the bioethical implications of heritable germline and non-heritable somatic genomic editing. We assessed student-learning outcomes and conclude that this learning module is an effective strategy for teaching undergraduates the fundamentals and application of CRISPR-cas gene editing technology and can be adapted to other genes and diseases that are currently being treated with CRISPR-cas technology., (© 2020 The Authors. Biochemistry and Molecular Biology Education published by Wiley Periodicals LLC on behalf of International Union of Biochemistry and Molecular Biology.)

The rapid development of molecular biotechnology presents a curricular challenge for educators trying to provide students with relevant coursework. A comprehensive biology education should also include opportunities for students to develop intellectual and technical skills through authentic research experiences. Integrating relevant and interesting research projects into their classes, however, can be a challenging task for instructors. To address these varied demands, we redesigned our existing molecular cloning course to incorporate an independent research project assessing calcium signaling. In the revised course, students use traditional and recombination-based cloning strategies to generate bacterial and mammalian expression vectors encoding CaMPARI, a novel fluorescent calcium indicator. Bacterially-expressed CaMPARI is used in protein quantification and purification assays. Students must also design their own research project evaluating the effect of chemotherapeutic agents on calcium signaling in a mammalian system. Revised and novel labs were designed to be modular, facilitating their integration into the course over 2 years. End-of-semester student evaluations were compared between years revealing a significant difference in students' perception of the course's difficulty between years. This change in attitude highlights potential pedagogical considerations that must be examined when introducing new material and activities into existing courses. Since calcium signaling is important for cellular process across diverse species, instructors may be able to develop research projects within their respective areas of interest. Integration of authentic research experiences into the curriculum is challenging; however, the framework described here provides a versatile structure that can be adapted to merge diverse instructor interests with evolving educational needs., (© 2020 International Union of Biochemistry and Molecular Biology.)