Author: "Benoit Pilmis" - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Benoit Pilmis"' showing total 130 results

Start Over Author "Benoit Pilmis"

130 results on '"Benoit Pilmis"'

51. Invasive Fungal Infection in Primary Immunodeficiencies Other Than Chronic Granulomatous Disease

Author: Stéphane Blanche, Anne Puel, Olivier Lortholary, Nizar Mahlaoui, Fanny Lanternier, Julie Toubiana, A. Garraffo, and Benoit Pilmis
Subjects: 0301 basic medicine, medicine.medical_specialty, business.industry, medicine.disease, 3. Good health, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Infectious Diseases, Chronic granulomatous disease, 030220 oncology & carcinogenesis, Tropical medicine, Immunology, medicine, Chronic mucocutaneous candidiasis, business, Dimorphic fungus
Abstract: Purpose of review We aimed to review invasive fungal infections complicating primary immunodeficiencies (PID).
Published: 2017
Full Text: View/download PDF

52. Disseminated Herpes Zoster During COVID-19

Author: Jean-Jacques Mourad, Elodie Ménage, Erwan Lelorc'h, Olivier Voisin, Benoit Pilmis, Nina Deluca, Marie-Françoise Borie, Sidonie Hubert, Charlotte Fite, Philippe Azria, and Annabelle Mahé
Subjects: 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), viruses, 030106 microbiology, herpes zoster, Context (language use), Case Reports, medicine.disease_cause, VZV, Virus, 03 medical and health sciences, 0302 clinical medicine, Pandemic, medicine, Disseminated herpes zoster, 030212 general & internal medicine, skin and connective tissue diseases, chickenpox, varicella-zoster virus, Chickenpox, SARS-CoV-2, business.industry, fungi, Varicella zoster virus, COVID-19, virus diseases, medicine.disease, Rash, Dermatology, body regions, Infectious Diseases, medicine.symptom, business
Abstract: During the SARS-CoV-2 pandemic, a variety of dermatological conditions were reported by physicians. Given the context, these lesions have been labeled as secondary to SARS-CoV-2 infection. We report the case of a recurrence of herpes zoster in a patient hospitalized with an SARS-CoV-2 infection. The rash occurred on the 15th day of hospitalization while the patient was recovering from a severe form. Local swab showed the presence of varicella-zoster virus within the vesicles. Dermatological symptoms secondary to COVID-19 have been frequently described. This is the first case that demonstrates the recurrence of herpes zoster during a SARS-CoV-2 infection.
Published: 2020
Full Text: View/download PDF

53. Emergence of Ceftriaxone Resistance during a Case of Pneumococcal Meningitis with Fatal Evolution

Author: C. Couzigou, J.C. Nguyen Van, Cédric Bruel, N Engrand, Thierry Lambert, Benoit Pilmis, Assaf Mizrahi, A. Le Monnier, Jean-Christophe Marvaud, Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Bactéries, Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Sud - Paris 11 (UP11), Groupe Hospitalier Paris Saint-Joseph (hpsj), Génomique fonctionnelle des champignons pathogènes des plantes (FungiPath), Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Subjects: Male, Penicillin binding proteins, Microbial Sensitivity Tests, medicine.disease_cause, Dexamethasone, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Cerebrospinal fluid, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Challenging Clinical Case in Antimicrobial Resistance, Streptococcus pneumoniae, Medicine, Humans, Penicillin-Binding Proteins, Pharmacology (medical), 030212 general & internal medicine, Pharmacology, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, 0303 health sciences, 030306 microbiology, business.industry, Meningitis, Pneumococcal, Ceftriaxone, Middle Aged, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Cephalosporins, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, Vasculitis, Meningitis, Streptococcus pneumoniae Meningitis, medicine.drug
Abstract: We report a case of a 62-year-old man treated for Streptococcus pneumoniae meningitis by ceftriaxone and dexamethasone. After neurological improvement, neurological degradation by vasculitis occurred, despite effective concentrations of ceftriaxone in the serum and cerebrospinal fluid (CSF). S. pneumoniae with increased MICs to third-generation-cephalosporins (3GC) was isolated from the ventricular fluid 10 days after the isolation of the first strain. Isolate analysis showed that a mutation in the penicillin-binding protein 2X (PBP2X) has occurred under treatment.
Published: 2020
Full Text: View/download PDF

54. Cutaneous listeriosis, a case series of 16 consecutive patients over 25 years

Author: Alexandra Moura, Alexandre Leclercq, Caroline Charlier, Pierre Thouvenot, Mylène M. Maury, Guillaume Vales, Marc Lecuit, Benoit Pilmis, Hélène Bracq-Dieye, Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie des Infections - Biology of Infection, Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), and DIAKITE, andrée
Subjects: Microbiology (medical), medicine.medical_specialty, skin, [SDV]Life Sciences [q-bio], Cutaneous listeriosis, Biology, medicine.disease_cause, 03 medical and health sciences, Listeria monocytogenes, listeriosis, Risk Factors, medicine, Humans, Listeria monocytogenes Word count: 866, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, 030306 microbiology, [SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology, Dermatology, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, England, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
Abstract: International audience
Published: 2020
Full Text: View/download PDF

55. Gut Microbiota, Antibiotic Therapy and Antimicrobial Resistance: A Narrative Review

Author: Alban Le Monnier, Jean-Ralph Zahar, and Benoit Pilmis
Subjects: 0301 basic medicine, Microbiology (medical), medicine.drug_class, medicine.medical_treatment, 030106 microbiology, Antibiotics, Review, Biology, Gut flora, Microbiology, law.invention, 03 medical and health sciences, Probiotic, Antibiotic resistance, law, Virology, Flora (microbiology), medicine, Antimicrobial stewardship, antimicrobial resistance, lcsh:QH301-705.5, gut microbiota, Prebiotic, medicine.disease, biology.organism_classification, 030104 developmental biology, lcsh:Biology (General), Dysbiosis, multidrug resistant pathogens
Abstract: Antimicrobial resistance is a major concern. Epidemiological studies have demonstrated direct relationships between antibiotic consumption and emergence/dissemination of resistant strains. Within the last decade, authors confounded spectrum activity and ecological effects and did not take into account several other factors playing important roles, such as impact on anaerobic flora, biliary elimination and sub-inhibitory concentration. The ecological impact of antibiotics on the gut microbiota by direct or indirect mechanisms reflects the breaking of the resistance barrier to colonization. To limit the impact of antibiotic therapy on gut microbiota, consideration of the spectrum of activity and route of elimination must be integrated into the decision. Various strategies to prevent (antimicrobial stewardship, action on residual antibiotics at colonic level) or cure dysbiosis (prebiotic, probiotic and fecal microbiota transplantation) have been introduced or are currently being developed.
Published: 2020

56. Intrauterine infection caused by nontyphoidal

Author: Bastien, Mollo, Claire Amaris, Hobson, Simon, Le Hello, Elie, Azria, Alban, Le Monnier, Benoit, Pilmis, and Assaf, Mizrahi
Subjects: Pregnancy, Salmonella, Sepsis, Salmonella Infections, Prevalence, Humans, Female, Anti-Bacterial Agents
Abstract: Although nontyphoidalWe reported a case analysis and subsequently conducted a systematic literature review of IUI caused by nontyphoidalIn literature nine cases have been reported, and were confirmed by the identification of a nontyphoidalNontyphoidal
Published: 2019

57. Failure of multiplex meningitis/encephalitis (ME) NAT during cryptococcal meningitis in solid organ recipients

Author: Yaye Senghor, Alexandre Alanio, Fanny Lanternier, Stéphane Bretagne, Romain Guery, Alban Le Monnier, Olivier Lortholary, Benoit Pilmis, and Marie-Elizabeth Bougnoux
Subjects: Male, 030230 surgery, Meningitis, Cryptococcal, 03 medical and health sciences, Immunocompromised Host, 0302 clinical medicine, Multiplex polymerase chain reaction, medicine, Humans, Multiplex, Diagnostic Errors, False Negative Reactions, Transplantation, medicine.diagnostic_test, business.industry, Nucleic acid test, Cryptococcosis, Organ Transplantation, Middle Aged, medicine.disease, Virology, Transplant Recipients, Infectious Diseases, Nat, 030211 gastroenterology & hepatology, Solid organ, Cryptococcal meningitis, business, Meningitis, Multiplex Polymerase Chain Reaction, Encephalitis
Abstract: Cryptococcal meningitis is a severe cause of central nervous system infections among immunocompromised solid organ transplant (SOT) patients. While new diagnostic methods as multiplex meningitis/encephalitis (ME) NAT (nucleic acid test) are increasingly used as a first-line tool in hospital practice, data in HIV-negative patients including SOT remain scarce. We report here false-negative results of multiplex NAT among SOT patients with proven cryptococcal meningitis.
Published: 2019

58. Clinical impact of rapid susceptibility testing on Mueller-Hinton Rapid-SIR directly from urine specimens

Author: Assaf Mizrahi, Michael Thy, Barbara Vidal, C. Couzigou, Benoit Pilmis, Steven Defarge, Olivier Jiang, Alban Le Monnier, and Jean-Claude Nguyen Van
Subjects: 0301 basic medicine, Microbiology (medical), Male, medicine.medical_specialty, Time Factors, Bacteriuria, medicine.drug_class, 030106 microbiology, Antibiotics, Context (language use), Urine, Microbial Sensitivity Tests, Fosfomycin, Asymptomatic, 03 medical and health sciences, Antimicrobial Stewardship, 0302 clinical medicine, Medical microbiology, Internal medicine, Gram-Negative Bacteria, medicine, Humans, 030212 general & internal medicine, Prospective Studies, Prospective cohort study, Pyuria, Aged, Aged, 80 and over, business.industry, General Medicine, Middle Aged, Anti-Bacterial Agents, Culture Media, Infectious Diseases, Nitrofurantoin, Urinary Tract Infections, Female, medicine.symptom, business, medicine.drug
Abstract: Urinary tract infection diagnosis and management generally involves a 48-h microbiological delay to obtain the antibiotic susceptibility test (AST) results. In the context of multidrug resistance, reducing the time to obtain AST results is an essential factor, allowing for more timely appropriate treatment. We conducted a single-centre prospective study on urinary samples meeting two criteria: significant leukocyturia > 50/mm3 and exclusive presence of Gram-negative bacilli on direct examination. AST were performed by direct inoculation on Mueller-Hinton Rapid-SIR (MHR-SIR) agar. We evaluated the time to antibiotic adaptation by the antimicrobial stewardship team according to rapid AST results. Patients were subsequently excluded from the study if asymptomatic bacteria were confirmed, or in the absence of clinical data. Seventy patients were included. Mean age of patients was 68.8 years (± 21.3). Empirical antibiotic treatment were mainly based on third generation cephalosporins (n = 33), fluoroquinolones (n = 15), beta-lactamin/beta-lactamase inhibitors (n = 7), fosfomycin and nitrofurantoin (n = 5, each). The average time to obtain results was 7.2 h (± 1.6 h). Adaptation of therapy following MHR-SIR was performed for 29 patients (41%) with early switch to oral antibiotics, de-escalation or escalation in respectively 72.3%, 30%, and 11% of cases. Time saving of MHR-SIR compared with the standard technique was 42.6 (± 16.7) h. This study showed that rapid antibiotic susceptibility test results, using MHR-SIR method directly from urine, can be obtained 40 h earlier than conventional AST. The study also demonstrated significant clinical impact on the selection and reduction of the antibiotic therapy spectrum.
Published: 2019

59. Impact of a multiplex PCR assay (FilmArray®) on the management of patients with suspected central nervous system infections

Author: Pierre Tattevin, C. Couzigou, Benoit Pilmis, Marine Cailleaux, Alban Le Monnier, Jean-Claude Nguyen Van, Barbara Vidal, Assaf Mizrahi, Julie Lourtet-Hascoët, and Lilian Alix
Subjects: 0301 basic medicine, Microbiology (medical), Adult, Male, medicine.medical_specialty, Paris, 030106 microbiology, medicine.disease_cause, Virus, 03 medical and health sciences, 0302 clinical medicine, Medical microbiology, Central Nervous System Infections, Listeria monocytogenes, Internal medicine, Multiplex polymerase chain reaction, medicine, Humans, Meningitis, 030212 general & internal medicine, Prospective Studies, Aged, Oligonucleotide Array Sequence Analysis, business.industry, General Medicine, Middle Aged, medicine.disease, Discontinuation, Infectious Diseases, Molecular Diagnostic Techniques, Enterovirus, Encephalitis, Female, Reagent Kits, Diagnostic, business, Multiplex Polymerase Chain Reaction
Abstract: Microbiological diagnosis of central nervous system (CNS) infections is challenging due to limited access to CNS samples, overlap between meningitis and encephalitis, and the multiplicity of pathogens potentially involved. We aimed to estimate the impact of a commercial multiplex PCR assay (FilmArray® meningitis/encephalitis) on the management of patients with suspicion of meningitis or encephalitis, in terms of time to diagnosis, antimicrobial agents use, duration of hospitalization, and costs. This prospective observational study was conducted at Saint Joseph Hospital (Paris, France) from December 2016 to December 2017. All CSF samples sent to the microbiology laboratory for suspicion of meningitis and/or encephalitis, with CSF cells count > 5 cells/μL, were tested by meningitis/encephalitis multiplex PCR assay. One hundred thirty patients were included. The multiplex PCR assay was positive in 33 patients (25%). Main pathogens found were Enterovirus (n = 12), Varicella-Zoster virus (n = 7), Herpes simplex virus-2 (n = 6), and Listeria monocytogenes (n = 3) as main pathogens. The multiplex PCR assay reduced time to microbiological diagnosis by 3.3 ± 1.6 days and allowed an earlier discontinuation of empirical anti-infective drugs in 42 patients (32%) and an earlier hospital discharge in 23 patients (18%), with an estimated saving of 82 hospital days overall, and a management cost reduction of 26,242 € (201 €/patient). The systematic use of the FilmArray® meningitis/encephalitis multiplex PCR assay may allow earlier diagnosis, earlier discontinuation of empirical treatment, reduced duration of stay, and costs reduction.
Published: 2019

60. Clinical evaluation of subcutaneous administration of cefepime

Author: Assaf Mizrahi, A. Le Monnier, G. Petitjean, Benoit Pilmis, and N. El Helali
Subjects: Male, medicine.drug_class, Cefepime, Injections, Subcutaneous, Cephalosporin, 03 medical and health sciences, Cmin, Subcutaneous injection, medicine, Retrospective analysis, Humans, Aged, Retrospective Studies, Aged, 80 and over, 0303 health sciences, 030306 microbiology, business.industry, Bacterial Infections, Anti-Bacterial Agents, Infectious Diseases, Treatment Outcome, Anesthesia, Plasma concentration, Female, business, Clinical evaluation, medicine.drug, Subcutaneous use
Abstract: Objectives Cefepime is a fourth-generation cephalosporin active against Pseudomonas aeruginosa and most Enterobacteriaceae. Intravenous (IV) administration is the standard route of prescription. However, subcutaneous administration (SC) may represent an interesting alternative. We aimed to evaluate SC administration of cefepime versus the IV route in geriatric patients. Patients and methods Multicenter retrospective analysis in patients treated with cefepime by SC route who underwent plasma concentration monitoring. Results Twelve patients were included in the SC group and matched to 12 patients in the IV group. The median and mean Cmin levels were 29.05 mg/L [14.2–48.2]; 33.4 mg/L (± 21.8) in the SC group and 31.9 mg/L [26.5–51.7]; 39.6 mg/L (± 27) (P = NS) in the IV group. No local SC administration-related complications were reported. No relapse was observed over six months of follow up. Conclusion Subcutaneous use of cefepime seems to have the same clinical and microbiological effectiveness as parenteral administration.
Published: 2019

61. Be careful about MICs to amoxicillin for patients with Streptococci-related infective endocarditis

Author: S. Corvec, Christophe Isnard, Hervé Jacquier, Audrey Mérens, Olivier Barraud, Marlène Amara, A. Le Monnier, Julie Lourtet-Hascoët, E. Thomas, C. Piau, Vincent Cattoir, Benoit Pilmis, Geneviève Héry-Arnaud, J.-R. Zahar, G. Péan de Ponfilly, David Boutoille, S. Reissier, E. Bonnet, Eric Farfour, Centre hospitalier Saint-Joseph [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Bactériologie, Virologie, Hygiène [CHU Limoges], CHU Limoges, CHU Pontchaillou [Rennes], Service de Microbiologie [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Groupe de Recherche sur l'Adaptation Microbienne (GRAM 2.0), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Centre Hospitalier de Versailles André Mignot (CHV), Hôpital d'Instruction des Armées Begin, Service de Santé des Armées, Hôpital Foch [Suresnes], Service de bactériologie et hygiène hospitalière [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Hôpital Lariboisière-Fernand-Widal [APHP], Hôpital Avicenne [AP-HP], Clinique Pasteur [Toulouse], Interactions hôte et microorganismes des épithéliums, Normandie Université (NU)-Normandie Université (NU), Service des maladies infectieuses et tropicales [CHU Nantes], and Centre hospitalier universitaire de Nantes (CHU Nantes)
Subjects: 0301 basic medicine, Male, Antibiotics, 0302 clinical medicine, Interquartile range, Risk Factors, Pharmacology (medical), 030212 general & internal medicine, ComputingMilieux_MISCELLANEOUS, Aged, 80 and over, Endocarditis, General Medicine, Middle Aged, Hospitals, 3. Good health, Cardiac surgery, Anti-Bacterial Agents, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infective endocarditis, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, France, medicine.drug, Microbiology (medical), Adult, medicine.medical_specialty, Adolescent, medicine.drug_class, 030106 microbiology, Microbial Sensitivity Tests, 03 medical and health sciences, Minimum inhibitory concentration, Young Adult, Internal medicine, Streptococcal Infections, medicine, Humans, Aged, Retrospective Studies, Native Valve Endocarditis, business.industry, Amoxicillin, Streptococcus, medicine.disease, Survival Analysis, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business
Abstract: Background A variety of microorganisms can cause infective endocarditis (IE), with Staphylococci and Streptococci accounting for the majority of cases. Streptococci are a common cause of community-acquired IE but few studies have focused on this subgroup of endocarditis. Methods A retrospective multicentre study was conducted between 2012 and 2017 in 12 hospital centres in France. Data were extracted from the local diagnosis-related group database and matched with microbiological results. After identification, the records were retrospectively analysed. Results A total of 414 patients with streptococcal endocarditis were included. The patients were predominantly male (72.8%) and the median age was 73.2 years (interquartile range [IQR] 61.3-80.9). The majority of patients (70.6%) had native valve endocarditis. Embolic complications were seen in 38.8% of patients. Viridans group Streptococci (VGS) and bovis-equinus group Streptococci (BGS) accounted for 52.4% and 34.5% of isolated strains, respectively. Minimum inhibitory concentrations (MICs) of amoxicillin were 2 mg/L for 59.6%, 27% and 1% of isolates, respectively. In-hospital mortality for patients with Streptococci-related IE was 17.8%. In multivariate analysis, the only factor associated with in-hospital mortality was MIC for amoxicillin between 0.25 and 2 mg/L (P = 0.04; OR = 2.23 [95% confidence interval (CI) 1.03-4.88]) whereas performance of cardiac surgery for IE was a protective factor (P = 0.001, OR = 0.23 [95% CI 0.1-0.56]). Conclusions IE remains a serious and deadly disease despite recent advances in diagnosis and treatment. Adaptation of antibiotic doses to MICs for amoxicillin and surgery may improve patient outcome.
Published: 2019
Full Text: View/download PDF

62. Clinical impact of rapid susceptibility testing on MHR-SIR directly from blood cultures

Author: C. Couzigou, Sophie Krob, Alban Le Monnier, Jean-Claude Nguyen Van, Julien Diep, Julie Lourtet-Hascoët, Claire Périllaud, Assaf Mizrahi, Michael Thy, Barbara Vidal, and Benoit Pilmis
Subjects: 0301 basic medicine, Microbiology (medical), Male, medicine.medical_specialty, Staphylococcus aureus, food.ingredient, medicine.drug_class, Urinary system, 030106 microbiology, Antibiotics, Bacteremia, Microbial Sensitivity Tests, medicine.disease_cause, Empirical antibiotic therapy, 03 medical and health sciences, 0302 clinical medicine, food, Enterobacteriaceae, Internal medicine, Antibiotic therapy, medicine, Agar, Humans, Pharmacology (medical), Blood culture, 030212 general & internal medicine, Aged, Pharmacology, Aged, 80 and over, medicine.diagnostic_test, business.industry, Case-control study, Enterobacteriaceae Infections, Middle Aged, Staphylococcal Infections, Anti-Bacterial Agents, Infectious Diseases, Blood Culture, Case-Control Studies, Female, business
Abstract: BackgroundIn a previous study, we demonstrated that rapid antibiotic susceptibility tests (ASTs) can be performed directly on blood culture samples tested on Mueller–Hinton Rapid agar (MHR-SIR) with a time delay of 6–8 h.ObjectivesUsing this rapid disc diffusion method, we analysed the clinical impact associated with rapid reporting of results in our hospital setting.MethodsAll patients with bloodstream infections (BSIs) related to Enterobacteriaceae or Staphylococcus aureus were prospectively included in the study. The rapid ASTs were performed by incubation of positive blood cultures on MHR-SIR for 6–8 h by direct inoculation according to BSAC recommendations.ResultsOne hundred and sixty-seven patients with BSIs were included as MHR-guided adaptation therapy cases. Eighty percent had Enterobacteriaceae-related BSIs, of which 12 (9%) were ESBL producers and 20% were S. aureus-related BSIs. A urinary or intra-abdominal infection was observed in 44.3% and 19.8%, respectively, of Enterobacteriaceae-related infections. The most frequent sources of infections for S. aureus BSIs were cutaneous and endovascular, in 43% and 23% of cases, respectively. Forty-four percent of the patients benefited from therapeutic modification according to the results of the MHR-SIR AST. Thus, empirical antibiotic therapy was modified by using antibiotic therapy that had too wide a spectrum or was unsuitable in 26% and 18% of cases, respectively. Compared with the 24 h required for the reference method, the median length of time to provision of susceptibility test results by MHR-SIR was 7 h.ConclusionsThis study showed a significant time saving (17 h) on the appropriateness of antibiotic prescription and demonstrated a significant impact regarding the choice and reduction of the spectrum of antibiotic therapy.
Published: 2019

63. A Mouse Model of Mild Clostridioides difficile Infection for the Characterization of Natural Immune Responses

Author: Assaf Mizrahi, Gauthier Péan de Ponfilly, Diane Sapa, Antonia Suau, Irène Mangin, Aurélie Baliarda, Sandra Hoys, Benoît Pilmis, Sylvie Lambert, Anaïs Brosse, and Alban Le Monnier
Subjects: Clostridioides difficile, immune response, colonization factor, toxins, gut microbiota, Biology (General), QH301-705.5
Abstract: (1) Background: We describe a model of primary mild-Clostridioides difficile infection (CDI) in a naïve host, including gut microbiota analysis, weight loss, mortality, length of colonization. This model was used in order to describe the kinetics of humoral (IgG, IgM) and mucosal (IgA) immune responses against toxins (TcdA/TcdB) and surface proteins (SlpA/FliC). (2) Methods: A total of 105 CFU vegetative forms of C. difficile 630Δerm were used for challenge by oral administration after dysbiosis, induced by a cocktail of antibiotics. Gut microbiota dysbiosis was confirmed and described by 16S rDNA sequencing. We sacrificed C57Bl/6 mice after different stages of infection (day 6, 2, 7, 14, 21, 28, and 56) to evaluate IgM, IgG against TcdA, TcdB, SlpA, FliC in blood samples, and IgA in the cecal contents collected. (3) Results: In our model, we observed a reproducible gut microbiota dysbiosis, allowing for C. difficile digestive colonization. CDI was objectivized by a mean weight loss of 13.1% and associated with a low mortality rate of 15.7% of mice. We observed an increase in IgM anti-toxins as early as D7 after challenge. IgG increased since D21, and IgA anti-toxins were secreted in cecal contents. Unexpectedly, neither anti-SlpA nor anti-FliC IgG or IgA were observed in our model. (4) Conclusions: In our model, we induced a gut microbiota dysbiosis, allowing a mild CDI to spontaneously resolve, with a digestive clearance observed since D14. After this primary CDI, we can study the development of specific immune responses in blood and cecal contents.
Published: 2024
Full Text: View/download PDF

64. Chronic Granulomatous Disease in Patients Reaching Adulthood: A Nationwide Study in France

Author: Julie Bardet, Olivier Hermine, Fanny Lanternier, Nicolas Noel, Isabelle Durieu, Agathe Masseau, Olivier Lortholary, Felipe Suarez, Jacinta Bustamante, Bertrand Dunogue, Hélène Salvator, Benoit Pilmis, Marie-Anne Gougerot-Pocidalo, Stéphane Blanche, Fanny Fouyssac, Nizar Mahlaoui, Marc Lecuit, Harry Sokol, Emilie Catherinot, Vincent Barlogis, Caroline Elie, Alain Fischer, Louis-Jean Couderc, Hélène Coignard-Biehler, Karima Amazzough, Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Référence Déficits Immunitaires Héréditaires (CEREDIH), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département d'Immunologie, hématologie et rhumatologie pédiatriques [Hôpital Necker-Enfants malades - APHP], CHU Necker - Enfants Malades [AP-HP], Génétique Humaine des Maladies Infectieuses (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'informatique médicale et biostatistiques [CHU Necker], Université Paris Descartes - Paris 5 (UPD5), Service de pneumologie [Hôpital Foch], Hôpital Foch [Suresnes], Service de Gastroentérologie et nutrition [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Hématologie et d'Oncologie Pédiatrique [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Service d'Onco-Hématologie Pédiatrique [CHU Grenoble], Centre Hospitalier Universitaire [Grenoble] (CHU), St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller University [New York], Service d'hématologie et immunologie, Université Paris Diderot - Paris 7 (UPD7)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Service de Pédiatrie et Hématologie Pédiatrique [CHU Timone, AP-HM], Hôpital de la Timone [CHU - APHM] (TIMONE), Service de médecine interne, Hôtel-Dieu-Centre hospitalier universitaire de Nantes (CHU Nantes), Service de médecine interne et de pathologie vasculaire [hôpital Lyon sud, HCL], Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Microorganismes et Barrières de l'Hôte (Equipe avenir), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Hématologie Adulte, Laboratory of molecular mechanisms of hematologic disorders and therapeutic implications (ERL 8254 - Equipe Inserm U1163), Chaire Médecine expérimentale (A. Fischer), Collège de France (CdF (institution)), Service d'immuno-hématologie pédiatrique [CHU Necker], The CEREDIH is supported by the French Association of Patients with Primary Immunodeficiencies and is funded by the French Ministry of Health. It additionally received unrestricted educational grants from Behring, Baxalta, Octapharma, and the patient associations AT-Europe and Trophée Guillaume., We thank all clinicians of the French national Centre de Référence des Déficits Immunitaires Héréditaires (CEREDIH) network for their help with this study. We also thank the CEREDIH staff: Chantal Andriamanga, Carolina Brito, Laurence Costes, Virginie Courteille, and Nathalie Devergnes., Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Service de pédiatrie, d'hématologie et d'oncologie [Hôpital de La Timone - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Collège de France - Chaire Médecine expérimentale (A. Fischer), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pneumologie [Hôpital Foch, Suresnes], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Saint-Antoine [APHP], Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), St Giles laboratory of Human Genetics and Infectious Diseases, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris]-Université Paris Diderot - Paris 7 (UPD7), Equipe avenir Microorganismes et Barrières de l'Hôte, and Collège de France (CdF)
Subjects: Male, 0301 basic medicine, Pediatrics, MESH: Registries, [SDV]Life Sciences [q-bio], medicine.medical_treatment, MESH: Granulomatous Disease, Chronic/diagnosis, Autoimmunity, Hematopoietic stem cell transplantation, chronic granulomatous disease, MESH: Granulomatous Disease, Chronic/complications, Granulomatous Disease, Chronic, 0302 clinical medicine, Chronic granulomatous disease, Cost of Illness, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Child, MESH: Bacterial Infections/etiology, MESH: Bacterial Infections/prevention & control, Registries, 030212 general & internal medicine, Child, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, High rate, MESH: France/epidemiology, MESH: Granulomatous Disease, Chronic/epidemiology, MESH: Bacterial Infections/epidemiology, MESH: Infant, Newborn, Age Factors, MESH: Mycoses/epidemiology, transition, Bacterial Infections, sequelae, MESH: Cost of Illness, MESH: Infant, Adult height, 3. Good health, Phenotype, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, Child, Preschool, Population Surveillance, MESH: Mycoses/drug therapy, MESH: Survival Analysis, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, France, Symptom Assessment, Microbiology (medical), medicine.medical_specialty, adulthood, Adolescent, MESH: Mycoses/prevention & control, primary immunodeficiency, MESH: Phenotype, MESH: Population Surveillance, 03 medical and health sciences, MESH: Cross-Sectional Studies, MESH: Antibiotic Prophylaxis, MESH: Autoimmunity, MESH: Mycoses/etiology, medicine, Humans, In patient, MESH: Bacterial Infections/drug therapy, Retrospective Studies, MESH: Adolescent, MESH: Age Factors, MESH: Humans, business.industry, MESH: Symptom Assessment, MESH: Granulomatous Disease, Chronic/mortality, MESH: Child, Preschool, Infant, Newborn, Infant, MESH: Retrospective Studies, Retrospective cohort study, Antibiotic Prophylaxis, medicine.disease, Survival Analysis, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, Cross-Sectional Studies, 030104 developmental biology, Mycoses, Respiratory failure, Primary immunodeficiency, business, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Background : Although prognosis of Chronic Granulomatous Disease (CGD) has greatly improved, few studies have focused on its long-term outcome. We studied the clinical course and sequelae of CGD patients diagnosed before age 16, at various adult time points. Method : Cross-sectional French nationwide retrospective study of patients screened through the National Reference Center for Primary Immunodeficiencies (CEREDIH) registry. Results : Eighty CGD patients (71 males [88.7%], 59 X-linked [73.7%], median age 23.9 years [minimum, 16.6; maximum, 59.9]) were included, Median ages at diagnosis and last follow-up were 2.52 and 23.9 years, respectively. Seven patients underwent hematopoietic stem cell transplantation. A total of 553 infections requiring hospitalization occurred in 2017 patient-years. The most common site of infection was pulmonary (31%). Aspergillus spp. (17%) and Staphylococcus aureus (10.7%) were the commonest pathogens. A total of 224 inflammatory episodes occurred in 71 patients, mainly digestive (50%). Their characteristics as well as their annual frequency did not vary before and after age 16. Main sequelae were a small adult height and weight and mild chronic restrictive respiratory failure. At age 16, only 53% of patients were in high school. After age 30 years, 9/13 patients were working. Ten patients died during adulthood. Conclusions : Adult CGD patients displayed similar characteristics and rates of severe infections and inflammatory episodes that those of childhood. The high rate of handicap has become a matter of medical and social consideration. Careful follow-up in centers of expertise is strongly recommended and an extended indication of curative treatment by HSCT should be considered.
Published: 2017
Full Text: View/download PDF

65. Biological markers in the diagnosis of tuberculous pleural effusion

Author: Benoit Pilmis, François Philippart, Stéphane Jouveshomme, and Bastien Mollo
Subjects: medicine.medical_specialty, Tuberculosis, Direct examination, Context (language use), Gastroenterology, Diagnosis, Differential, 03 medical and health sciences, 0302 clinical medicine, Adenosine deaminase, Internal medicine, Biopsy, medicine, Humans, 030212 general & internal medicine, Diagnostic Techniques and Procedures, biology, medicine.diagnostic_test, business.industry, Tuberculosis, Pleural, General Medicine, medicine.disease, Pleural Effusion, Pleurisy, 030221 ophthalmology & optometry, biology.protein, Etiology, business, Biomarkers, Rifampicin, medicine.drug
Abstract: Tuberculosis is one of the main etiologies to evoke in the context of lymphocyte pleurisy. However, diagnosis is difficult and is based on mycobacteriology that is not enough sensitive and time-consuming, or on histology that requires invasive biopsy gesture. This literature review, carried out from Medline, summarizes the main meta-analyzes, reviews, and originator publications in English on biomarkers, classic and more innovative, studied for the diagnosis of tuberculous pleurisy. Among the immuno-biochemical markers, interferon-γ (IFN-γ), isoenzyme of adenosine deaminase 2 (ADA2) and total adenosine deaminase (ADA) seem the most relevant with respective sensitivities of 89% (87-91), 97.2% (95 to 98.7) and 92% (90-93) and specificities of 97% (96-98), 94.2% (91.8 to 96) and 90% (89-91). About molecular biology, PCR Xpert MTB/RIF has a sensitivity of 46.4% (26.3 to 67.8), which is much higher than the direct examination, while providing rapid diagnostic confirmation, with a specificity of 99.1% (95.2 to 99.8), and a resistance to rifampicin screening. The release assay of interferon-γ (IGRA) is less effective with a sensitivity of 75% (69-81) and a specificity of 82% (75-88) in blood and a sensitivity of 80% (74-86%) and a specificity of 72% (64-80) in pleural fluid. Other biomarkers (including several cytokines) might have an interest but are still under evaluation. These innovative methods, particularly the determination of ADA and the use of PCR Xpert MTB/RIF should find their place in the diagnostic algorithm of TB pleurisy.
Published: 2017
Full Text: View/download PDF

66. New clinical phenotypes of fungal infections in special hosts

Author: Olivier Lortholary, Fanny Lanternier, Anne Puel, and Benoit Pilmis
Subjects: 0301 basic medicine, Microbiology (medical), medicine.medical_treatment, Deep dermatophytosis, Fungi, Immunologic Deficiency Syndromes, Immunosuppression, Context (language use), General Medicine, Biology, Aspergillosis, medicine.disease, 03 medical and health sciences, Phenotype, 030104 developmental biology, Infectious Diseases, Mycoses, Host-Pathogen Interactions, Cryptococcosis, Immunology, medicine, Pneumocystosis, Humans, Chronic mucocutaneous candidiasis, Immunodeficiency
Abstract: Incidence of invasive fungal infections increases over time with the rise in at-risk populations; in particular, patients with acquired immunodeficiencies due to immunosuppressive therapies such as anti-tumour necrosis factor-α (TNF-α) treatment, cirrhosis or burns. Some primary immunodeficiencies (PID) can also predispose selectively to invasive fungal diseases. Conversely, some atypical fungal diseases can reveal new PID. Deep dermatophytosis, Candida central nervous system infections or gastrointestinal disease, or disseminated phaeohyphomycosis-revealed CARD9 deficiency. Most patients with inherited chronic mucocutaneous candidiasis were found to carry STAT1 gain-of-function mutations. The spectrum of fungal susceptibility and clinical presentation varies according to the PID. Among acquired immunodeficiencies, immunosuppressive treatments such as TNF-α blocker therapy, which has revolutionized autoimmune disorder treatment, may be complicated by endemic mycosis, aspergillosis, pneumocystosis or cryptococcosis. Burn patients with damaged skin barrier protection are susceptible to severe Candida infections and filamentous fungal infections (such as Aspergillus spp., Mucorales). Moreover, patients with cirrhosis are at increased risk of fungal infections. Therefore, physicians should think of any potential underlying acquired or inherited immunodeficiency in a patient developing an atypical fungal infection, or of a potential fungal disease in the context of an atypical presentation in specific hosts.
Published: 2016
Full Text: View/download PDF

67. Classement pragmatique des antibiotiques en fonction de leur spectre et de leur impact écologique à des fins éducatives : résultats d’une enquête Delphi pour le jeu éducatif « Dawaa »

Author: S. Abbara, Solen Kernéis, C. Cazanave, Benoit Pilmis, A. Lefort, Raphaël Lepeule, C. D’humières, L. Luong Nguyen, S. Jauréguiberry, and Vincent Dubée
Subjects: Infectious Diseases
Abstract: Introduction Un jeu educatif a ete developpe pour les etudiants en medecine visant a leur apprendre le bon usage des antibiotiques et les sensibiliser a l’antibioresistance. Le jeu s’appuie sur un classement des antibiotiques, en fonction de leur spectre et de leur potentiel de selection de souches resistantes. Ce classement est difficile a realiser en l’absence de hierarchie claire entre les antibiotiques ni de donnees solides, surtout en dehors des betalactamines. L’objectif de ce travail etait de parvenir a un consensus d’experts. Materiels et methodes Nous avons forme un conseil scientifique comprenant six infectiologues, une specialiste en medecine interne et une microbiologiste, parmi lesquels six universitaires. Vingt-trois antibiotiques ont ete selectionnes pour le classement. Chaque expert a ete invite a attribuer des points de penalite de 1 a 5 a chaque antibiotique. Un processus de concertation Delphi avec des tours successifs a ete mene pour parvenir a un accord. Plus de 70 % de reponses similaires etaient necessaires pour parvenir a un consensus. Resultats L’etude a ete realisee entre fevrier et juillet 2019. Un consensus pour tous les antibiotiques, a l’exception des carbapenemes, a ete atteint apres trois tours. Aucun expert n’a attribue une penalite de 1 a un antibiotique. Une penalite de 2 a ete attribuee a la penicilline M, la penicilline A, la piperacilline, les cephalosporines de 1re et 2e generation, la pristinamycine, la rifampicine, la vancomycine, les aminosides, les macrolides, le metronidazole, la fosfomycine, les cyclines et l’aztreonam. Une penalite de 3 a ete attribuee a l’amoxicilline–clavulanate, aux cephalosporines de 3e et 4e generations et a la clindamycine. Une penalite de 4 a ete attribuee a la piperacilline–tazobactam, a la ticarcilline–clavulanate et aux fluoroquinolones. Un debat entre les experts a ete organise au debut et a la fin du processus de concertation. Il est apparu que les antibiotiques ont ete classes en premier lieu en fonction de l’etendue de leur spectre, et qu’une penalite de 4 a ete attribuee aux antibiotiques connus pour selectionner des souches resistantes. Les experts ont finalement attribue une penalite de 5 aux carbapenemes pour apprendre aux etudiants a considerer ces molecules, a spectre extremement large, comme un traitement de dernier recours. Conclusion Grâce a un processus de concertation Delphi avec des experts, nous avons pu attribuer une penalite a la plupart des antibiotiques utilises en clinique, y compris les antibiotiques autres que les betalactamines, pour le jeu. Ces penalites ont d’abord pris en compte l’etendue du spectre des antibiotiques, puis l’impact ecologique suppose, et transmis le message que les carbapenemes sont a conserver comme traitement de dernier recours.
Published: 2020
Full Text: View/download PDF

68. Response to Afonso et al

Author: J.-R. Zahar and Benoit Pilmis
Subjects: business.industry, Sepsis, Pseudomonas aeruginosa, Pediatrics, Perinatology and Child Health, Humans, Library science, Medicine, Child, business, Retrospective Studies
Published: 2020
Full Text: View/download PDF

69. Listeria monocytogenes-associated endovascular infections: A study of 71 consecutive cases

Author: Guillaume Vales, Grégory Jouvion, Caroline Charlier, Gabrielle Coulpier, Olivier Disson, Marc Lecuit, Benoit Pilmis, Alexandre Leclercq, Pierre Thouvenot, Mylène M. Maury, Michka Shoai-Tehrani, Hélène Bracq-Dieye, Olivier Robineau, Laboratoire des Listeria, Centre National de Référence des Listeria, et Centre collaborateur de l’OMS pour la listériose d’origine alimentaire (CNR - CCOMS), Institut Pasteur [Paris], Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier de Tourcoing, Biologie des Infections - Biology of Infection, Neuropathologie expérimentale - Experimental neuropathology, Institut Pasteur [Paris]-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Paris 5 (UPD5), This study received financial support from Institut Pasteur, Inserm and Santé Publique France., We thank Dr. Isabelle Serre (Gui Chauliac University Hospital, Montpellier, France), Dr. Valérie Hervieu (Edouard Herriot University Hospital, Lyon, France), Dr. Beatrice Luciani-Laillou (Périgueux, France) and Dr. Luc Marcellin (Hautepierre University Hospital, Strasbourg, France), David Hardy and Magali Tichit (Experimental Neuropathology Unit, Institut Pasteur, Paris) for their contribution to histopathology studies, as well as Giulia Nigro (Molecular Microbial Pathologenesis Unit, Institut Pasteur, Paris)., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Université Paris Descartes - Paris 5 (UPD5), and DIAKITE, andrée
Subjects: 0301 basic medicine, Male, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Prosthesis, medicine.disease_cause, Intracardiac injection, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Risk Factors, Medicine, Listeriosis, 030212 general & internal medicine, Hospital Mortality, Aged, 80 and over, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Endocarditis, Middle Aged, 3. Good health, [SDV] Life Sciences [q-bio], Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Heart Valve Prosthesis, Epidemiological Monitoring, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Female, France, Microbiology (medical), medicine.medical_specialty, 030106 microbiology, 03 medical and health sciences, Aneurysm, Listeria monocytogenes, Humans, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Aged, Retrospective Studies, business.industry, Retrospective cohort study, Endocarditis, Bacterial, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Surgery, Heart failure, Concomitant, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Background: Listeria monocytogenes-associated endovascular infections are not well characterized. Methods: Retrospective study of 71 culture-proven cases reported to the French National Reference Center for Listeria from 1993 to 2018. Results: Seventy-one cases were identified: 42 with vascular aneurysms/prosthetic infections, 27 with endocarditis, 2 with both. Fifty-eight were men (82%); median age was 75 years [46-92]; 93% reported co-morbidities (66/71), including 50% with immunosuppressive conditions. Vascular infections consisted of infected aneurysms (68%) or prosthetic graft infections (32%); vascular rupture was reported in 25/42 (60%). Tissue samples grew L. monocytogenes in 98% (43/44) and blood cultures in 64% (27/42). Endo-carditis cases involved prosthetic or native valves or intracardiac devices in respectively 62% (18/29), 28% (8/29) and 10% (3/29). Infected valves were aortic (62%, 16/26), mitral (31%, 8/26) or both (8%, 2/26); 38% patients required surgery; 45% displayed heart failure; 17% had concomitant neurolisteriosis. In-hospital mortality in vascular infections was 12% (5/42) and 41% (12/29) for Lm-associated endocarditis. Conclusions: Endovascular listeriosis is a rare but severe infection. It manifests as vascular infections and endocarditis, mostly in older patients with vascular or cardiac valve prosthetic devices and co-morbidities. Mortality in Lm-associated endocarditis is twice higher than with other pathogens, requiring prompt recognition and treatment.
Published: 2019
Full Text: View/download PDF

70. Prevention and Treatment of Mold Infections

Author: Olivier Lortholary, Claire Aguilar, Shahid Husain, and Benoit Pilmis
Subjects: Voriconazole, medicine.medical_specialty, Aspergillus, biology, Liver toxicity, business.industry, Incidence (epidemiology), medicine.medical_treatment, Mold infection, biology.organism_classification, medicine.disease, Internal medicine, Epidemiology, medicine, Lung transplantation, Disseminated disease, business, medicine.drug
Abstract: Invasive fungal infections remain a major issue in solid organ transplantation (SOT). The incidence and type of fungal infection vary among organ groups. While invasive candidiasis is the most frequent fungal infections in all SOT, invasive mold infections (IMIs) present as a predominant type of invasive fungal infections in lung transplantation. In lung transplantation, a major challenge is to distinguish invasive infection from colonization of airways. Among other SOT recipients, liver transplant recipients show some specific features of IMI with more frequent disseminated disease. The main mold involved in invasive diseases in SOT recipients is Aspergillus spp. The treatment of choice of IA is voriconazole. Side effects such as liver toxicity and skin toxicity, particularly frequent in SOT recipients, should be kept in mind. Prophylactic strategies vary among organ groups, according to the incidence of mold infection, local epidemiology, and possible side effects of the antifungal drugs. However, modalities and efficacy of prophylactic measures are still debated.
Published: 2019
Full Text: View/download PDF

71. Ventilator-associated pneumonia related to ESBL-producing gram negative bacilli

Author: Benoit Pilmis and Jean-Ralph Zahar
Subjects: 0301 basic medicine, Carbapenem, medicine.medical_specialty, medicine.drug_class, business.industry, 030106 microbiology, Antibiotics, Ventilator-associated pneumonia, General Medicine, Gram negative bacilli, Review Article, medicine.disease, bacterial infections and mycoses, Intensive care unit, law.invention, 03 medical and health sciences, Pneumonia, Carriage, law, Pharmacodynamics, Internal medicine, medicine, business, medicine.drug
Abstract: Ventilator-associated pneumonia (VAP) is one of the most frequent cause of intensive care unit (ICU) acquired infections. The worldwide spreading of extended spectrum beta-lactamase producing enterobacteriaceae (ESBL-PE) represents a major problem encountered more and more frequently in ICU. Among ICU patients, between 5% to 25% are ESBL-PE carriers. Whereas, previous carriage is the major risk factors associated with VAP related to ESBL-PE, among carriers, only 5% to 20% will develop a VAP related to ESBL-PE. Also, diagnosis and therapeutic delay are associated with length of stay and higher morbidity, and mortality, therefore, early identification of patients at risk of ESBL-PE related infections is crucial for early implementation of effective antibiotic therapy. VAP related to ESBL-PE should be considered in: previous colonized patients in case of late onset pneumonia and/or when several antibiotic courses precede the infectious episode or even in patients with shock. Among non-colonized patients, if VAP occurs, the risk being related to ESBL-PE is less than 1%. In the future, new rapid microbiological diagnostic tests will allow an early diagnosis. According to recent data, empirical antibiotic therapy should be based on carbapenems. Other alternative antibiotic classes could be used for de-escalation. However, several pharmacodynamic and pharmacokinetics precautions should be taken to achieve drug concentrations at site of infection and except to cure the infected patient.
Published: 2018

72. Failure of voriconazole therapy due to acquired azole resistance in Aspergillus fumigatus in a kidney transplant recipient with chronic necrotizing aspergillosis

Author: Emilie Catherinot, Benoit Pilmis, Stéphane Bretagne, Anne Scemla, Olivier Lortholary, Alexandre Alanio, Vincent Jullien, Dea Garcia-Hermoso, Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Université Paris Descartes - Paris 5 (UPD5), Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris], Hôpital Foch [Suresnes], Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)
Subjects: 0301 basic medicine, Drug, media_common.quotation_subject, infectious disease, 030106 microbiology, Neutropenia, Aspergillosis, clinical research/practice, Aspergillus fumigatus, 03 medical and health sciences, chemistry.chemical_compound, Immunology and Allergy, Medicine, Pharmacology (medical), infection and infectious agents ‐ fungal, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, media_common, Voriconazole, Transplantation, biology, business.industry, Mortality rate, medicine.disease, biology.organism_classification, antibiotic: antifungal, 3. Good health, chemistry, Infectious disease (medical specialty), Immunology, Caspofungin, business, medicine.drug
Abstract: International audience; Invasive aspergillosis (IA) affects the lungs and disseminates mostly in patients with neutropenia and/or patients who are receiving immunosuppressive and steroid therapies. Despite progress in the diagnosis of and therapy for IA, it is still characterized by a high mortality rate. Currently, voriconazole is considered as the standard therapy for IA. Over recent years, triazole-resistant Aspergillus fumigatus isolates have emerged in the environment due to the use of fungicidal agricultural products, with the risk of developing IA related to a resistant isolate. However, resistance may also develop in patients who are undergoing long-term triazole therapy, particularly in the setting of chronic forms of pulmonary aspergillosis. Herein we describe a kidney transplant recipient who failed to respond to voriconazole therapy due to acquired resistance secondary to the appearance of a de novo mutation (Y121F) in the cyp51A gene during chronic necrotizing pulmonary aspergillosis. The infecting isolate acquired voriconazole resistance in 8 months despite plasma concentrations within the recommended range of the drug, necessitating lobectomy in association with a new antifungal strategy consisting of liposomal amphotericin and caspofungin with a good outcome over 36 months.
Published: 2018
Full Text: View/download PDF

73. Performance of rapid antimicrobial susceptibility testing by disk diffusion on MHR-SIR agar directly on urine specimens

Author: Gauthier Péan de Ponfilly, Alban Le Monnier, Jean-Claude Nguyen Van, Simon Perreau, Assaf Mizrahi, Julien Diep, Benoit Pilmis, Barbara Vidal, Louise Ruffier d'Epenoux, C. Couzigou, and Claire Périllaud-Dubois
Subjects: 0301 basic medicine, Microbiology (medical), food.ingredient, Time Factors, 030106 microbiology, Antimicrobial susceptibility, Urine, Microbial Sensitivity Tests, 03 medical and health sciences, 0302 clinical medicine, food, Anti-Infective Agents, Enterobacteriaceae, Agar, Humans, 030212 general & internal medicine, Prospective Studies, Gram, Chromatography, Chemistry, Enterobacteriaceae Infections, Reproducibility of Results, General Medicine, Standard methods, Culture Media, Infectious Diseases, Urinary Tract Infections
Abstract: The standard method for the diagnosis of urinary tract infections is urine culture that requires 18–48 h for the identification of the bacteria and an additional 24 h until the results of antimicrobial susceptibility testing (AST) are available. We evaluated here a rapid AST method by disc diffusion performed directly on urine samples with a delay of 8 h. A total of 245 urine samples with monobacterial Gram negative observed on microscopy were tested in parallel by two AST methods. Rapid AST method was performed directly on urine samples using Rapid Mueller-Hinton (MHR-SIR) with 8-h incubation before reading and standard method was performed as usual. We compared the categorical agreement and the correlation between the diameters obtained by standard method and by MHR-SIR directly on urine samples. Over the 5285 tested combinations, we observed 5172 (97.9%) categorical agreement, 82 (1.5%) minor errors, 17 (0.3%) major errors, and 14 (0.3%) very major errors. Our results showed an excellent categorical agreement and correlations between diameters for MHR-SIR and standard methods. MHR-SIR performed directly on urine samples with monomicrobial Enterobacteriacae can predict the result of overall AST profile in 8 h with reliable results. The main advantage of MHR-SIR is that it offers the possibility of obtaining results 40 h earlier than conventional AST. The cost is estimated for less than 6 USD for 16 antibiotics, chosen by the microbiologist.
Published: 2018

74. Prospective evaluation of rapid antimicrobial susceptibility testing by disk diffusion on Mueller-Hinton rapid-SIR directly on blood cultures

Author: Claire Périllaud, C. Couzigou, Alban Le Monnier, Jean-Claude Nguyen Van, Assaf Mizrahi, Julien Diep, Gauthier Péan de Ponfilly, Benoit Pilmis, Julie Lourtet-Hascoët, and B. Vidal
Subjects: 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Staphylococcus aureus, food.ingredient, Time Factors, medicine.drug_class, 030106 microbiology, Antibiotics, Bacteremia, Pilot Projects, medicine.disease_cause, Sepsis, 03 medical and health sciences, 0302 clinical medicine, Antibiotic resistance, food, Enterobacteriaceae, Disk Diffusion Antimicrobial Tests, Internal medicine, Drug Resistance, Bacterial, medicine, Agar, Humans, Blood culture, 030212 general & internal medicine, Prospective Studies, Diagnostic Errors, biology, medicine.diagnostic_test, business.industry, Enterobacteriaceae Infections, General Medicine, Staphylococcal Infections, biology.organism_classification, Antimicrobial, medicine.disease, Anti-Bacterial Agents, Infectious Diseases, Early Diagnosis, Blood Culture, business
Abstract: Background With the worldwide spread of antibiotic resistance, delivering antibiotic susceptibility test (AST) results in a timely manner represents a major challenge. In cases of sepsis, rapid AST may facilitate early optimization of empiric antibiotic therapy. Disc diffusion is a well-standardized AST method, however 16 to 24 h are required to achieve an overall AST profile according to antimicrobial societies. Methods In this prospective pilot study, we evaluated the performance of Mueller-Hinton-Rapid-SIR (MHR-SIR) agar after 6–8 h of incubation in comparison with standard MH agar after 16 h of incubation directly on positive blood cultures caused by Enterobacteriaceae and Staphylococcus aureus from routine clinical microbiology. A total of 133 positive blood samples including 110 Enterobacteriaceae (83%) and 23 Staphylococcus aureus (17%) were tested in parallel by two direct AST methods, each using EUCAST breakpoints. For each combination bacterium and antibiotic, we compared the categorical agreement and the correlation between the diameters obtained by MHR-SIR and by standard MH. Results Our results showed 97.7% categorical agreement for Enterobacteriaceae, with 1.4% minor errors, 0.4% major errors and 0.5% very major errors. For S. aureus, we observed 97.8% categorical agreement, 1.9% minor errors, 0.3% major errors and no very major errors. Conclusion Our results showed excellent categorical agreement and correlations between diameters for MHR-SIR and standard MH methods. MHRSIR can predict the result of overall AST profile within 6–8 h with reliable results. AST is obtained on the same day the blood culture becomes positive, with a very moderate cost.
Published: 2018

75. Clinical impact of rapid bacterial identification by MALDI-TOF MS combined with the bêta-LACTA™ test on early antibiotic adaptation by an antimicrobial stewardship team in bloodstream infections

Author: A. Mizrahi, G. Péan de Ponfilly, A. Le Monnier, C. Couzigou, Benoit Pilmis, and Jonas Amzalag
Subjects: 0301 basic medicine, Microbiology (medical), Time Factors, medicine.drug_class, 030106 microbiology, Antibiotics, Cephalosporin, Bacteremia, Microbial Sensitivity Tests, beta-Lactamases, Microbiology, 03 medical and health sciences, Antimicrobial Stewardship, Antibiotic therapy, medicine, Antimicrobial stewardship, Humans, Blood culture, Prospective Studies, General Immunology and Microbiology, medicine.diagnostic_test, Bacteria, Cephalosporin Resistance, business.industry, food and beverages, General Medicine, medicine.disease, Anti-Bacterial Agents, Cephalosporins, Infectious Diseases, Blood Culture, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, business
Abstract: Bloodstream infections (BSI) can potentially be life-threatening infections and are associated with a high crude mortality, moreover with an inappropriate first-line antibiotic therapy. Bacterial resistance is more and more frequently observed. New strategies of BSI management are urgently needed.During an 18-months period, we prospectively evaluated the clinical impact of rapid bacterial identification by MALDI-TOF MS technology combined with an antimicrobial stewardship team (AST) intervention. Furthermore, during an 8-months period, we combined this strategy with the rapid detection of third-generation cephalosporin (3GC) resistance by the Bêta-LACTA™ test (BLT) directly on blood cultures. We then evaluated the theoretical impact of BLT on antibiotic therapy adaptation and establishment of infection control measures.A total of 335 blood cultures were enrolled during the study. MALDI-TOF MS gave accurate identification for 301 blood cultures (89,8%) and led to early antibiotic therapy adaptation for 73 episodes (21.8%). BLT was performed on 141 blood cultures, revealing 28 3GC-resistant bacteria (19.9%). Twenty-one patients (75%) received a non-adapted first-line treatment. The antibiotic therapy adaptation was delayed by 28.1 hours and the establishment of infection control measures by 35 hours with antimicrobial susceptibility testing, compared to the theoretical adaptation with BLT result.These tools can be included in a strategy of bloodstream infections management for a better patient care, optimizing and saving the use of antibiotics, notably carbapenems as well as diminishing the spread of multi-drug resistant bacteria by applying rapidly infection control measures.
Published: 2018

76. Infective endocarditis: Clinical presentation, etiology, and early predictors of in-hospital case fatality

Author: A. Laincer, R. Cador, A. Mizrahi, N. El Helali, A. Le Monnier, Benoit Pilmis, J.C. Nguyen Van, P. Abassade, and C. Couzigou
Subjects: Male, medicine.medical_specialty, Prosthesis-Related Infections, Embolism, Comorbidity, 030204 cardiovascular system & hematology, 03 medical and health sciences, Postoperative Complications, 0302 clinical medicine, Internal medicine, Case fatality rate, Epidemiology, Diabetes Mellitus, medicine, Humans, Endocarditis, Hospital Mortality, 030212 general & internal medicine, Cardiac Surgical Procedures, Aged, Retrospective Studies, Aged, 80 and over, Heart Valve Prosthesis Implantation, business.industry, Retrospective cohort study, Middle Aged, Prognosis, medicine.disease, Anti-Bacterial Agents, Surgery, Infectious Diseases, Heart Valve Prosthesis, Infective endocarditis, Etiology, Kidney Failure, Chronic, Female, France, business
Abstract: Objective We aimed to assess the clinical presentation, microbial etiology and outcome of patients presenting with infective endocarditis (IE). Patients and methods We conducted a four-year retrospective study including all patients presenting with IE. Results We included 121 patients in the study. The median age was 74.8 years. Most patients had native valve IE (57%). Staphylococcus aureus accounted for 24.8% of all IE. Surgery was indicated for 70 patients (57.9%) but actually performed in only 55 (44.7%). Factors associated with surgery were younger age ( P = 0.002) and prosthetic valve IE ( P = 0.001). Risk factors associated with in-hospital mortality were diabetes mellitus (OR = 3.17), chronic renal insufficiency (OR = 6.62), and surgical indication (OR = 3.49). Mortality of patients who underwent surgery was one sixth of that of patients with surgical indication who did not have the surgery ( P
Published: 2016
Full Text: View/download PDF

77. Aminoglycoside use in a pediatric hospital: there is room for improvement—a before/after study

Author: Mélanie Houot, Martine Postaire, Benoit Pilmis, Valérie Thépot-Seegers, Cyrielle Potier, Clémence Suard, and Jean-Ralph Zahar
Subjects: Male, 0301 basic medicine, Clinical audit, medicine.medical_specialty, Pediatrics, Time Factors, medicine.drug_class, Urinary system, 030106 microbiology, Antibiotics, Drug Prescriptions, 03 medical and health sciences, 0302 clinical medicine, Pediatric hospital, medicine, Humans, Prospective Studies, 030212 general & internal medicine, Dosing, Medical prescription, Child, Prospective cohort study, Clinical Audit, business.industry, Aminoglycoside, Hospitals, Pediatric, Aminoglycosides, Pediatrics, Perinatology and Child Health, Emergency medicine, Female, business, Follow-Up Studies
Abstract: Aminoglycoside prescriptions were rarely evaluated in children care facilities. Because of risk of toxicity, these narrow spectrum antibiotics are commonly misused. In this study, we evaluate aminoglycoside prescription and assess the impact of an information campaign on modalities of prescription and monitoring practices in a pediatric hospital. This prospective study, before/after diffusion of local recommendations, has been conducted over 6 months. All computerized prescriptions were analyzed. A semi-passive diffusion of local recommendations to prescribers allowed researchers to differentiate between a pre-intervention (P1) and post-intervention period (P2). Endpoints were the improvement of administered doses (mg/kg), modalities of administration, treatment duration, indications, and the presence of pharmacological monitoring. Three hundred and ten prescriptions were analyzed (P1 = 163, P2 = 147). Most common sites of infection treated were as follows: joint-bone (33 %), urinary tract (17 %) and intra-abdominal (15 %). Among all prescriptions, respectively, 12 and 13 % were avoidable. Short-duration treatment and single daily dosing seem to be widely achieved, but despite an improvement between the two periods, 45 % of prescribed doses in P2 were still below our recommendations (77 % in P1).The semi-passive diffusion of recommendations has not improved significantly medical practices. Active diffusion with a regular monitoring could be useful to improve the use of aminoglycosides.• Misuse of aminoglycosides has been frequently described and evaluated in adult hospitals. • This misuse could be explained by their nephrotoxicity and their low therapeutic index. What is New: • Through this study, conducted in a pediatric hospital, we highlighted that practitioners misunderstand the aminoglycoside pharmacokinetic and pharmacodynamic targets and 12.3 % of aminoglycoside prescriptions could be avoided. • Finally, we showed that a semi-passive diffusion of local recommendations is not enough to improve aminoglycoside prescriptions.
Published: 2016
Full Text: View/download PDF

78. ESBL-producing enterobacteriaceae-related urinary tract infections in kidney transplant recipients: incidence and risk factors for recurrence

Author: Zahar, Scemla A, Benoit Pilmis, Mamzer Mf, Olivier Lortholary, Legendre C, and Olivier Join-Lambert
Subjects: Adult, Male, Microbiology (medical), medicine.medical_specialty, Time Factors, Urinary system, Urology, Bacteriuria, urologic and male genital diseases, Kidney transplant, beta-Lactamases, Young Adult, Enterobacteriaceae, Recurrence, Risk Factors, Internal medicine, Humans, Medicine, Prospective Studies, Escherichia coli Infections, Kidney transplantation, Aged, Retrospective Studies, Aged, 80 and over, General Immunology and Microbiology, biology, business.industry, Incidence, Incidence (epidemiology), Enterobacteriaceae Infections, Retrospective cohort study, General Medicine, Middle Aged, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Antimicrobial, Kidney Transplantation, Transplant Recipients, female genital diseases and pregnancy complications, Anti-Bacterial Agents, Infectious Diseases, Urinary Tract Infections, Female, France, business
Abstract: Urinary tract infections (UTIs) represent the first cause of bacterial infections in renal transplant recipients. In a period of increasing resistance to antimicrobial agents, the factors leading to the development of UTI in previously urinary colonized renal transplant recipients as well as the factors associated with recurrence of UTIs have to be determined. The aims of this retrospective study were (1) to assess the incidence of extended-spectrum beta-lactamase-producing enterobacteriaceae (ESBL-PE)-related UTI in kidney transplant recipients, (2) to identify factors associated with ESBL-PE infection and (3) to determine the risk factors for recurrence.We included all kidney transplant recipients admitted in our hospital between January 2009 and January 2012 who had a monobacterial ESBL-PE UTI or bacteriuria.During the study period, 659 patients underwent kidney transplantation; 72 patients had ESBL-PE bacteriuria, representing a 10.9% prevalence, and among the latter 34 (47.2%) presented an ESBL-PE-related UTI. Fourteen patients (41.2%) experienced a UTI relapse associated with two factors: advanced age (p = 0.032) and persistent bacteriuria 48 h after appropriate antibiotic therapy (p = 0.04). No other risk factor for recurrence was found, including the presence and management of a ureteral stent during the first UTI, causative microorganisms, or diabetes mellitus.In this specific population, regarding the risk of relapse there is an urgent need for prospective studies to test the best treatment strategy.
Published: 2015
Full Text: View/download PDF

79. Acute reversible pancreatitis induced by posaconazole

Author: Claire Rouzaud, Olivier Lortholary, Hélène Coignard-Biehler, Benoit Pilmis, Vincent Jullien, and Fanny Lanternier
Subjects: Pharmacology, Microbiology (medical), medicine.medical_specialty, Posaconazole, business.industry, medicine.disease, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Infectious Diseases, Internal medicine, medicine, Pancreatitis, 030211 gastroenterology & hepatology, Pharmacology (medical), 030212 general & internal medicine, business, medicine.drug
Published: 2016
Full Text: View/download PDF

80. Recent advances in the understanding and management of mucormycosis

Author: Fanny Lanternier, Benoit Pilmis, Olivier Lortholary, Alexandre Alanio, Université Paris Descartes - Paris 5 (UPD5), Sorbonne Université (SU), Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut Pasteur [Paris], Centre hospitalier Saint-Joseph [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Diderot - Paris 7 (UPD7), Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris], Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], The author(s) declared that no grants were involved in supporting this work. FL is a speaker for bureau Gilead, Basilea, MSD. Concultancy Basilea. She received research grant from Gilead Sciences.OL has been a consultant for Gilead Sciences, a speakers’ bureau for Pfeizer, Novartis, Astellas, Merck, and Gilead Sciences, and has received research grants from Gilead Sciences., Thanks to Dea Garcia-Hermoso for reading the manuscript and providing strain photographs., Groupe hospitalier Paris Saint-Joseph - Hôpital, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)
Subjects: 0301 basic medicine, medicine.medical_specialty, Antifungal Agents, 030106 microbiology, Liposomal amphotericin B, Computed tomography, Diagnostic tools, Polymerase Chain Reaction, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Reverse Halo sign, medicine, Animals, Humans, Mucormycosis, 030212 general & internal medicine, General Pharmacology, Toxicology and Pharmaceutics, Intensive care medicine, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, General Immunology and Microbiology, medicine.diagnostic_test, business.industry, Isavuconazole, General Medicine, medicine.disease, 3. Good health, PCR, business, Tomography, X-Ray Computed
Abstract: International audience; Mucormycoses were difficult-to-manage infections owing to limited diagnostic tools and therapeutic options. We review here advances in pathology understanding, diagnostic tools including computed tomography, and serum polymerase chain reaction and therapeutic options.
Published: 2018
Full Text: View/download PDF

81. Pulmonary manifestations in adult patients with chronic granulomatous disease

Author: Felipe Suarez, Bruno Crestani, Olivier Lortholary, Olivier Hermine, Hélène Salvator, Jean-François Dreyfus, Raphael Borie, Louis-Jean Couderc, Nizar Mahlaoui, Marie-Anne Gougerot-Pocidalo, Emilie Catherinot, Margarita Hurtado-Nedelec, Colas Tcherakian, Benoit Pilmis, Alain Fischer, Bertrand Dunogue, Elisabeth Rivaud, Fanny Fouyssac, and Isabelle Durieu
Subjects: Adult, Lung Diseases, Male, Pulmonary and Respiratory Medicine, Pediatrics, medicine.medical_specialty, Adolescent, Biopsy, Disease, Granulomatous Disease, Chronic, Asymptomatic, Cohort Studies, Young Adult, Chronic granulomatous disease, Anti-Infective Agents, Pneumonia, Bacterial, Humans, Immunologic Factors, Medicine, Young adult, Lung, Retrospective Studies, Membrane Glycoproteins, Lung Diseases, Fungal, business.industry, Incidence (epidemiology), NADPH Oxidases, Retrospective cohort study, Middle Aged, medicine.disease, Pneumonia, Asymptomatic Diseases, NADPH Oxidase 2, Immunology, Primary immunodeficiency, Female, medicine.symptom, business
Abstract: Chronic granulomatous disease (CGD) is a primary immunodeficiency caused by failure of superoxide production in phagocytic cells. The disease is characterised by recurrent infections and inflammatory events, frequently affecting the lungs. Improvement of life expectancy now allows most patients to reach adulthood. We aimed to describe the pattern of pulmonary manifestations occurring during adulthood in CGD patients.This was a retrospective study of the French national cohort of adult patients (≥16 years old) with CGD.Medical data were obtained for 67 adult patients. Pulmonary manifestations affected two-thirds of adult patients. Their incidence was significantly higher than in childhood (mean annual rate 0.22 versus 0.07, p=0.01). Infectious risk persisted despite anti-infectious prophylaxis. Invasive fungal infections were frequent (0.11 per year per patient) and asymptomatic in 37% of the cases. They often required lung biopsy for diagnosis (10 out of 30). Noninfectious respiratory events concerned 28% of adult patients, frequently associated with a concomitant fungal infection (40%). They were more frequent in patients with the X-linked form of CGD. Immune-modulator therapies were required in most cases (70%).Respiratory manifestations are major complications of CGD in adulthood. Noninfectious pulmonary manifestations are as deleterious as infectious pneumonia. A specific respiratory monitoring is necessary.
Published: 2015
Full Text: View/download PDF

82. Helicobacter bilis-Associated Suppurative Cholangitis in a Patient with X-Linked Agammaglobulinemia

Author: Agnès Ferroni, Justine Dautremer, Nicolas Degand, Julie Bruneau, Fanny Lanternier, Marc Lecuit, Olivier Hermine, Olivier Lortholary, Stéphane Blanche, Benoit Pilmis, Xavier Nassif, Laboratoire de Microbiologie [AP-HP Necker], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Service d'AnatomoPathologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'Hématologie Adulte, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Université Paris Descartes - Paris 5 (UPD5), Service d'immuno-hématologie pédiatrique [CHU Necker], We thank Agnès Labigne, Unité de Pathogénie bactérienne des muqueuses, Institut Pasteur, Paris, France, for her help in this case report., Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], and Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP]
Subjects: 0301 basic medicine, Male, MESH: Helicobacter Infections/pathology, MESH: Agammaglobulinemia/pathology, [SDV]Life Sciences [q-bio], Azithromycin, Bruton’s disease, Gastroenterology, Medical microbiology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Helicobacter, Immunology and Allergy, MESH: Anti-Bacterial Agents/therapeutic use, MESH: Agammaglobulinemia/drug therapy, MESH: Genetic Diseases, X-Linked/pathology, medicine.diagnostic_test, biology, MESH: Helicobacter Infections/drug therapy, Suppurative cholangitis, Genetic Diseases, X-Linked, 3. Good health, Anti-Bacterial Agents, cholangitis, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Liver, MESH: Young Adult, Liver biopsy, [SDV.IMM]Life Sciences [q-bio]/Immunology, medicine.drug, Helicobacter bilis, DNA, Bacterial, medicine.medical_specialty, 030106 microbiology, Immunology, MESH: Genetic Diseases, X-Linked/drug therapy, DNA, Ribosomal, Helicobacter Infections, MESH: Liver/pathology, 03 medical and health sciences, Young Adult, MESH: Helicobacter/genetics, Internal medicine, medicine, MESH: Helicobacter Infections/microbiology, Humans, Colitis, MESH: DNA, Ribosomal/genetics, MESH: Humans, business.industry, MESH: Agammaglobulinemia/microbiology, MESH: Genetic Diseases, X-Linked/microbiology, biology.organism_classification, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, MESH: DNA, Bacterial/genetics, agammaglobulinemia, Metronidazole, MESH: Cholangitis/pathology, 030104 developmental biology, MESH: Cholangitis/drug therapy, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, MESH: Cholangitis/microbiology
Abstract: International audience; Helicobacter bilis is a commensal bacterium causing chronic hepatitis and colitis in mice. In humans, enterohepatic Helicobacter spp. are associated with chronic hepatobiliary diseases. Purpose : We aimed at understanding the microbial etiology in a patient with X-linked agammaglobulinemia presenting with suppurative cholangitis. Methods : 16S rDNA PCR directly performed on a liver biopsy retrieved DNA of H. bilis. Results : Clinical outcome resulted in the normalization of clinical and biological parameters under antibiotic treatment by a combination of ceftriaxone, metronidazole, and doxycyclin followed by a 2-week treatment with moxifloxacin and a 2-month treatment with azithromycin. Conclusion : In conclusion, these data suggest a specific clinical and microbiological approach in patients with humoral deficiency in order to detect H. bilis hepatobiliary diseases.
Published: 2017
Full Text: View/download PDF

83. How Should We Treat Hospital-Acquired and Ventilator-Associated Pneumonia Caused by Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae?

Author: Benoit Pilmis, Jean-François Timsit, and Jean-Ralph Zahar
Subjects: 0301 basic medicine, Pulmonary and Respiratory Medicine, medicine.medical_specialty, medicine.drug_class, 030106 microbiology, Antibiotics, Critical Care and Intensive Care Medicine, beta-Lactamases, law.invention, 03 medical and health sciences, Enterobacteriaceae, law, Risk Factors, Internal medicine, polycyclic compounds, medicine, Humans, Intensive care medicine, Cross Infection, biology, business.industry, Ventilator-associated pneumonia, Enterobacteriaceae Infections, Pneumonia, Ventilator-Associated, Pneumonia, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Intensive care unit, Icu admission, Anti-Bacterial Agents, Intensive Care Units, Carbapenems, Carrier State, Surveillance culture, business, Empiric therapy
Abstract: Hospital-acquired and ventilator-associated pneumonia (HAP/VAP) due to extended-spectrum β-lactamase–producing Enterobacteriaceae (ESBL-PE) represent a growing problem. Indeed, ESBL-PE is endemic in many countries, and 5 to 25% of intensive care unit (ICU) patients are ESBL-PE carrier on ICU admission. ESBL-PE HAP/VAP is associated with a higher mortality than HAP/VAP due to susceptible Enterobacteriaceae because the resistance profile decreases the adequacy rate of empiric therapy. ESBL-PE should be considered in the empirical treatment in case of the high burden of ESBL-PE in the unit, in the case of previous ESBL-PE colonization, when the HAP/VAP occurs late, and in patients with shock. A negative active systematic surveillance culture on rectal swab reduced the risk of ESBL-PE VAP to less than 1%. Rapid diagnostic tests are now able to confirm the presence of ESBL-PE in VAP within 24 hours; new molecular methods will provide results within few hours.Adequate treatment usually required carbapenems. The alternative β-lactams such as β-lactams/β-lactamases inhibitor combinations could be proposed as a step-down therapy according to the antibiotic susceptibility result. Optimization of pharmacokinetics requires high dosage and continuous or prolonged infusions for β-lactams. When the patient is stabilized, a therapy of duration 7 to 8 days is recommended.
Published: 2017

84. Implementation of Alere i Influenza AB point of care test for the diagnosis of influenza in an ED

Author: B. Vidal, A. Le Monnier, J.C. Nguyen Van, C. Caisso, C. Couzigou, E. Trabattoni, G. Pean de Ponfilly, Assaf Mizrahi, Benoit Pilmis, Bruno Lina, V. Le, and Olivier Ganansia
Subjects: 0301 basic medicine, Adult, Male, medicine.medical_specialty, Point-of-care testing, 030106 microbiology, Physical examination, 03 medical and health sciences, 0302 clinical medicine, Influenza, Human, Clinical endpoint, medicine, Humans, 030212 general & internal medicine, Prospective Studies, Prospective cohort study, Aged, Retrospective Studies, medicine.diagnostic_test, business.industry, Reverse Transcriptase Polymerase Chain Reaction, Incidence (epidemiology), Incidence, Retrospective cohort study, General Medicine, Emergency department, Middle Aged, Influenza B virus, Influenza A virus, Point-of-Care Testing, Emergency medicine, Cohort, DNA, Viral, Emergency Medicine, Female, France, business, Emergency Service, Hospital
Abstract: This study aimed to evaluate the impact of implementing rapid point-of-care testing (POCT) with the Alere i Influenza AB in an emergency department (ED) during an influenza epidemic.Direct nasal swabs were prospectively collected following the physical examination of patients aged18years who presented to the ED of a tertiary hospital in France with influenza-like illness (ILI) symptoms (N=301) between February 1st and March 31st, 2016, which coincided with an influenza epidemic. Laboratory-based testing (standard of care) was used to obtain a diagnosis in February 2016 (pre-POCT cohort) and positive results were confirmed using polymerase chain reaction. The primary endpoint was patient time in the ED.A total of 169 and 132 patients participated in the pre-POCT phase and POCT phase respectively. A significantly higher proportion of patients received a positive diagnosis in the POCT cohort compared with the pre-POCT cohort (31% versus 5.3%, P0.01). Mean time spent in the ED and hospitalization rate were significantly lower in the POCT cohort (6.06h versus 4.15h, P=0.03, and 44.4% versus 9.7%, P=0.02, respectively). Despite similar rates in the prescription of antibiotics and antiviral therapies, the proportion of patients who were referred for additional tests was significantly lower in the POCT cohort (78.1% versus 62.1%, P=0.003, and 80.5% versus 63.6%, P=0.01, respectively).The Alere i Influenza AB POCT reduced the length of stay in ED, the hospitalization rates, and the number of additional diagnostic tests compared with standard of care testing.
Published: 2017

85. Infections caused by naturally AmpC-producing Enterobacteriaceae: Can we use third-generation cephalosporins? A narrative review

Author: T Delerue, Benoit Pilmis, A. Le Monnier, Jean-Ralph Zahar, E Carbonnelle, H Morel, and Assaf Mizrahi
Subjects: 0301 basic medicine, Microbiology (medical), medicine.drug_class, 030106 microbiology, Cephalosporin, Colony Count, Microbial, Providencia, beta-Lactamases, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Enterobacteriaceae, Drug Resistance, Bacterial, polycyclic compounds, medicine, Pharmacology (medical), 030212 general & internal medicine, biology, business.industry, Enterobacteriaceae Infections, General Medicine, Enterobacter, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Anti-Bacterial Agents, Cephalosporins, Citrobacter freundii, Infectious Diseases, Serratia marcescens, bacteria, Morganella morganii, business, Bacteria
Abstract: The burden of antibiotic-resistant infections among Gram-negative bacteria is increasing. Resistance to third-generation cephalosporins (3GCs) in Enterobacteriaceae is mainly conferred by the acquisition of β-lactamases or by deregulation of natural genetically-encoded β-lactamase enzymes. Enterobacteriaceae such as Enterobacter spp., Serratia marcescens, Citrobacter freundii, Providencia spp. and Morganella morganii (ESCPM group) possess chromosomally-encoded inducible AmpC β-lactamases. AmpC can be overproduced as a response to β-lactam antibiotic exposure or by constitutive dysfunction of the AmpC regulation system. This overproduction can lead to the inactivation of 3GCs. Based on small clinical studies, international guidelines and expert recommendations suggest that 3GCs should be avoided as definitive therapy for infections caused by ESCPM group organisms. In this narrative review, we discuss the published literature and evaluate the risk related to 3GC use in the case of documented ESCPM infection.
Published: 2020
Full Text: View/download PDF

86. Endocarditis due to Neisseria mucosa: Case report and review of 21 cases

Author: Benoit Pilmis, Agnès Lefort, Xavier Nassif, Olivier Lortholary, Caroline Charlier, Marc Lecuit, and Olivier Join-Lambert
Subjects: Microbiology (medical), Pathology, medicine.medical_specialty, Infectious Diseases, business.industry, medicine, Endocarditis, medicine.disease, business, Neisseria mucosa
Published: 2014
Full Text: View/download PDF

87. Impact d’un programme de bon usage des carbapénèmes dans un hôpital universitaire français

Author: Benoit Pilmis, J. Poline, Pierre Frange, Julie Toubiana, Martine Postaire, Olivier Lortholary, J.-R. Zahar, and Perrine Parize
Subjects: Infectious Diseases
Abstract: Introduction Les programmes de bon usage des antibiotiques ont pour objectif de reduire l’usage excessif d’antibiotiques a large spectre. Afin de limiter l’utilisation des carbapenemes dans notre hopital universitaire, l’equipe mobile d’infectiologie (EMI) intervient aupres du prescripteur dans les 24–72 h qui suivent la prescription. L’objectif de cette etude etait d’evaluer l’utilisation des carbapenemes dans nos services de pediatrie et de medecine adulte. Materiels et methodes Chaque nouvelle prescription de carbapenemes etait identifiee par un systeme d’alerte informatique provenant de la pharmacie et prospectivement evaluee par l’EMI afin proposer une desescalade. Les donnees cliniques et microbiologiques des patients ont ete collectees, et l’intervention de l’EMI dans tous les services de pediatrie et de medecine adulte ont ete analysees sur une duree de un an (2014). Resultats Au total, 383 prescriptions de carbapenemes concernant 158 enfants et 140 adultes ont ete etudiees. La majorite des patients avaient une pathologie sous-jacente (97 %) et 51 % avaient un portage connu d’enterobacteries productrices de beta-lactamase a spectre etendu (EBLSE). La majorite des prescriptions concernait les services de reanimation (26 %) et etait instauree pour des infections urinaires (29 %) et pulmonaires (19 %). Les prescriptions de carbapenemes etaient probabilistes dans 269 (70 %) cas. Les 264 infections documentees etaient majoritairement causees par Klebsiella spp. (33 %) ; 51 % etaient des dues a des EBLSE et 8 % a des bacteries resistantes aux carbapenemes. Il existait des alternatives aux carbapenemes identifiees sur l’antibiogramme dans 59 % des cas. L’EMI a evalue 323 prescriptions, 38 % d’entre elles etaient considerees comme non appropriees et necessitaient soit un arret du traitement antibiotique (8 %), soit une desescalade (30 %). Les prescriptions inappropriees etaient principalement observees dans les services pediatriques (46 % vs 34 %, p Conclusion Un traitement par carbapenemes etait generalement instaure chez des patients pris en charge pour infection grave ou avec des facteurs de risque de resistance aux antibiotiques. Neanmoins, plus d’un tiers des prescriptions etaient considerees comme inappropriees et arretees par l’EMI, principalement dans les services pediatriques. Cette etude suggere que le role de l’EMI est essentiel afin de mieux controler l’utilisation des antibiotiques en pediatrie et par consequent la resistance aux antibiotiques.
Published: 2018
Full Text: View/download PDF

88. Impact de l’utilisation d’une PCR multiplex type FilmArray ® dans le diagnostic des infections suspectées du système nerveux central à l’hôpital

Author: Benoit Pilmis, Marine Cailleaux, Jean-Claude Nguyen, A. Mizrahi, A. Le Monnier, J. Lourtet, and C. Couzigou
Subjects: Infectious Diseases
Abstract: Introduction Les infections du systeme nerveux central comme les meningites et encephalites representent actuellement un veritable challenge diagnostique et therapeutique du fait d’une mortalite, de sequelles et d’un cout socio-economique important. Actuellement, les donnees cliniques, la microbiologie standard et les donnees d’imageries sont le plus souvent insuffisantes pour l’obtention d’un diagnostic etiologique precis. Dans ce contexte des outils de diagnostic rapide, des infections bacteriennes, virales, parasitaires et fongiques impliquees dans les infections neuromeningees ont ete developpes en tant qu’outils d’aide au diagnostic precoce. Materiels et methodes Il s’agit d’une etude de monocentrique retrospective menee entre juin 2016 a decembre 2017. Tous les patients ayant beneficie de la realisation d’un test FilmArray® dans un contexte de suspicion de meningite ou de meningo-encephalite ont ete inclus. Les donnees cliniques, biologiques et microbiologiques ont ete retrospectivement recueillies afin d’evaluer l’impact de cette innovation sur la prise en charge de nos patients. Resultats Cent trente patients ont beneficie de la realisation d’un test FilmArray® durant la periode de l’etude. La moyenne d’âge etait de 50,1 ans. Les services les plus prescripteurs de ce test et dont l’indication etait validee par un binome microbiologiste/infectiologue etaient le service d’accueil des urgences et le service de neurologie. Un traitement antibiotique ou antiviral empirique avait ete debute chez 55,7 % des patients. Au niveau de la ponction lombaire, quatre prelevements presentaient un examen direct positif et huit une culture bacterienne positive. Le test FilmArray® etait positif dans 33 cas, soit 25,1 % des tests realises. Grâce a la realisation de ce test, les traitements ont pu etre adaptes en mediane 3 jours plus rapidement que lors de l’utilisation de la strategie diagnostique classique. Son utilisation a permis, une diminution des prescriptions d’anti-infectieux non adaptes, de limiter les prescriptions d’examens complementaires superflus, de diminuer les durees d’hospitalisation et d’optimiser la cotation des sejours. Conclusion Le test FilmArray® peut etre un outil d’aide au diagnostic des meningites/meningoencephalites communautaires aigues. De plus, son utilisation raisonnee peut permettre sans surcout une amelioration de la prise en charge des patients presentant une infection potentiellement severe.
Published: 2018
Full Text: View/download PDF

89. Impact clinique de l’antibiogramme rapide MHR-SIR (i2a) directement à partir des urines

Author: Olivier Jiang, Benoit Pilmis, J.C. Nguyen Van, A. Le Monnier, S. Defarge, and Michael Thy
Subjects: Infectious Diseases
Abstract: Introduction Le diagnostic d’infection urinaire necessite en general un delai en microbiologie de 48 h afin d’obtenir l’antibiogramme. Dans le contexte de la multiresistance bacterienne, la reduction du delai d’obtention des resultats de l’antibiogramme (ATB) constitue un enjeu essentiel pour eviter les echecs therapeutiques. Dans une etude precedente, nous avons demontre que l’ATB rapide peut etre obtenu directement a partir d’echantillons d’urines testes sur MHR-SIR (Rapid Mueller-Hinton) avec une excellente correlation a la methode standard dans un delai de 8 h (Eur J Clin Microbiol Infect Dis 2019 Jan ;38(1):185–189. doi: 10,1007/s10096-018-3413-5). L’objectif de ce travail est d’evaluer l’impact clinique de cette pratique. Materiels et methodes Les prelevements urinaires ont ete selectionnes sur les deux criteres suivants : leucocyturie significative > 5,104/mL et presence uniquement de bacilles a Gram negatif a l’examen direct. La cytologie urinaire etait realisee par cytometrie de flux sur automate Sysmex UF-500i (bioMerieux, France). Les ECBU ont ete realises en ensemencant une gelose MHR-SIR (i2a, France) a l’ecouvillon directement a partir de l’urine selon les recommandations de la British Society for Antimicrobial Chemotherapy et la lecture a ete effectuee sur le systeme SIR scan 2000 Automatic system (i2a, France). Resultats Un total de 107 patients presentant une infection urinaire ont ete inclus L’âge moyen des patients etait de 70 ans [52–80], sexe ratio F :H 2,1 avec une CRP mediane de 104 [39–145] et un score de Charlson de 3 [1–5,5] Les ECBU montraient une leucocyturie mediane de 380/mm3 [192–1497] et une bacteriurie mediane de 106UFC/ml [105–107]. Le traitement antibiotique a ete instaure pour 65 % des patients en utilisant majoritairement les cephalosporines de 3e generation (33), les fluoroquinolones (15), les inhibiteurs de beta-lactamases (7), la fosfomycine (5), la nitrofurantoine (5). Le temps moyen d’obtention des resultats etait de 7,2 heures (± 1,6 heures). L’adaptation au MHR-SIR a ete realisee pour 29 patients (27 %) avec une escalade dans 7 % et une desescalade dans 20,7 % et un relais precoce par voie orale dans 72,3 % des cas. Le gain de temps du MHR-SIR par rapport a la technique standard etait de 42,6 h. Conclusion Cette etude montre que les resultats d’antibiogramme rapide par la technique MHR-SIR directement a partir des urines peuvent etre obtenus 40 heures plus tot par rapport a la technique standard et demontre un impact clinique important sur le choix et la reduction du spectre de l’antibiotherapie.
Published: 2019
Full Text: View/download PDF

90. Urinary tract infections to Enterococcus spp.: Risk factors and treatments

Author: R. Garreau, J. Lourtet, Benoit Pilmis, H. Roux, and A. Le Monnier
Subjects: business.industry, Urinary system, Biochemistry (medical), Clinical Biochemistry, Enterococcus spp, Medicine, General Medicine, business, Biochemistry, Microbiology
Published: 2019
Full Text: View/download PDF

91. Caractéristiques des méningites à Klebsiella pneumoniae et Klebsiella oxytoca

Author: Philippe Morand, F. Janvier, Benoit Pilmis, S. Corvec, Antoine Grillon, P. Huriez, C. Le Brun, Emmanuelle Bille, Vincent Cattoir, and A. Le Monnier
Subjects: Infectious Diseases
Abstract: Introduction Decrire les caracteristiques des patients pris en charge dans un contexte de meningite a Klebsiella pneumoniae ou Klebsiella oxytoca. Materiels et methodes Etude retrospective, multicentrique incluant 35 patients ayant presente une meningite documentee a Klebsiella pneumoniae ou Klebsiella oxytoca. Resultats Trente-cinq patients ayant presente une meningite documentee a Klebsiella sp ont ete inclus : 27 adultes et 8 enfants. Parmi les patients, 51,4 % (18/35) etaient hospitalises dans un contexte neurochirurgical (craniectomie, hydrocephalie, derivation ventriculaire externe ou peritoneale, tumerectomie). Chez les adultes, 44,4 % avaient une tumeur solide (12/44), 7,4 % avaient une hepatopathie chronique (2/27), 7,4 % presentaient une intoxication ethylique chronique (2/27), et 3,7 % etaient diabetiques (1/27). Parmi les 8 enfants, un seul presentait une tumeur solide. Le tableau clinique associait de la fievre chez 85,7 % des patients (30/35), des troubles neurologiques focaux pour 40 % des patients (14/35), des nausees ou des vomissements dans 34,3 % des cas (12/35), des cephalees pour 28,6 % des patients (10/35), une raideur de nuque dans 28,6 % des cas (10/35), un choc septique pour 14,3 % (5/35) et une crise comitiale chez 11,4 % des patients (4/35). Il existait des localisations secondaires dans 17,1 % des cas (6/35) : 66,6 % de pneumopathies (4/6), 16,7 % d’abces hepatiques (1/6), et 16,7 % d’abces cerebelleux (1/6). Il existait une bacteriemie associee dans 34,3 % des cas (12/35) et une bacteriurie dans 8,6 % des cas (3/35). L’examen direct du liquide cerebro-spinal etait positif dans 65,7 % des cas (23/35). Sur les 35 souches de Klebsiella, on retrouvait 94,3 % de Klebsiella pneumoniae (33/35), et 5,7 % de Klebsiella oxytoca (2/35), et 20 % des souches etaient productrice de beta-lactamase a spectre etendu (7/35). Vingt patients ont beneficie d’une imagerie cerebrale (scanner ou IRM), qui retrouvait une anomalie dans 75 % des cas (15/20). Du point de vue therapeutique, l’antibiotherapie probabiliste reposait sur une cephalosporines, une carbapeneme ou l’association piperacilline-tazobactam dans respectivement 42,9 %, 25,8 % et 5,7 % des cas. Apres documentation, l’antibiotherapie prescrite etait principalement une cephalosporine (65,7 %) ou une carbapeneme dans respectivement 65,7 % et 11,5 % des cas. Une corticotherapie etait associee au traitement antibiotique dans 8,6 % des cas (3/35). Au total, le taux de mortalite des meningites a Klebsiella sp. s’elevait a 31,4 % (11/35), et 11,4 % des patients presentaient des sequelles a distance (4/35). Conclusion Les meningites a Klebsiella sp. sont tres peu decrites et pourtant la mortalite est elevee. Elles semblent survenir avant 1 an, ou aux alentours de 55 ans, et peut survenir chez les patient sans antecedent ou terrain predisposant.
Published: 2019
Full Text: View/download PDF

92. Les échinocandines : mise au point

Author: Olivier Lortholary, Benoit Pilmis, A. Lopes, and Caroline Charlier
Subjects: Infectious Diseases, Pharmacology (medical)
Abstract: Resume Les echinocandines sont devenues en 2013 une famille majeure de l’arsenal therapeutique antifongique. Elles representent une famille d’antifongiques administrables par voie intraveineuse agissant par inhibition de la synthese du β-(1,3)- d -glucane, composant essentiel de la paroi fongique de nombreux champignons. Trois echinocandines semi-synthetiques sont a ce jour commercialisees : la caspofungine, la micafungine et l’anidulafungine. Elles ont un spectre large mais sont inactives sur Cryptococcus sp. et les mucorales. Elles possedent un effet « anti-biofilm » qui leur confere un interet particulier dans le traitement des infections a Candida sp. sur materiel etranger notamment sur catheter. Leur efficacite a ete demontree chez l’adulte et egalement en pediatrie, y compris chez les nouveau-nes et les prematures. Leur tolerance est excellente, leurs interactions medicamenteuses sont peu importantes et limitees, facilitant l’utilisation de ces molecules chez des patients fragiles et polymedicamentes. Leur cout eleve et leur impact ecologique potentiel doivent cependant en limiter l’usage a une « juste prescription ».
Published: 2013
Full Text: View/download PDF

93. Mucormycoses

Author: Benoit Pilmis, Fanny Lanternier, and Olivier Lortholary
Subjects: Gynecology, medicine.medical_specialty, Amphotéricine B, business.industry, Mucormycosis, medicine, General Medicine, business, medicine.disease, General Biochemistry, Genetics and Molecular Biology
Abstract: Les mucormycoses sont des infections fongiques invasives survenant principalement sur des terrains immunodeprimes (hemopathie maligne, transplantation d’organe solide, diabete) et grevees d’une lourde morbi-mortalite. Les localisations les plus frequentes de ces infections fongiques angio-invasives sont pulmonaires, rhino-cerebrales, cutanees et enfin disseminees. Le traitement de ces infections repose sur un tryptique associant un controle strict des facteurs de risque, notamment une equilibration rigoureuse d’un diabete sous-jacent, une prise en charge chirurgicale precoce avec exerese des tissus necroses et, enfin, une prise en charge medicamenteuse avec utilisation de polyenes a forte dose (amphotericine B liposomale ≥ 5 mg/kg/jour). Il parait actuellement legitime de proposer un traitement d’attaque par polyene pour une duree minimale de 6 a 8 semaines et jusqu’a resolution des lesions, suivi d’un traitement d’entretien, pour les patients restant immunodeprimes, par posaconazole avec une surveillance etroite des taux residuels.
Published: 2013
Full Text: View/download PDF

94. New Approaches to Manage Infections in Transplant Recipients: Report From the 2023 GTI (Infection and Transplantation Group) Annual Meeting

Author: Alexandra Serris, Julien Coussement, Benoît Pilmis, Victoire De Lastours, Aurélien Dinh, François Parquin, Eric Epailly, Florence Ader, Olivier Lortholary, Emmanuel Morelon, Nassim Kamar, Edouard Forcade, David Lebeaux, Jérôme Dumortier, Filomena Conti, Agnes Lefort, Anne Scemla, and Hannah Kaminski
Subjects: muli-drug resistant bacteria, antimicrobial resistance, antimicrobial stewardship, antifungal therapy, urinary tract infection, Specialties of internal medicine, RC581-951
Published: 2023
Full Text: View/download PDF

95. Legionnaire's Disease in Compromised Hosts

Author: Fanny Lanternier, Sophie Jarraud, Emilie Catherinot, Benoit Pilmis, Florence Ader, Olivier Lortholary, Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Pathogenèse des légionelles- Legionella pathogenesis (LegioPath), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Foch [Suresnes], Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Necrosis, Legionella, 030106 microbiology, TNF blockers, HIV Infections, Disease, 03 medical and health sciences, Immunocompromised Host, 0302 clinical medicine, Immunity, Neoplasms, Epidemiology, Tobacco, medicine, Humans, 030212 general & internal medicine, Legionnaire's disease, Legionellosis, biology, business.industry, TLR-5, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Transplant Recipients, 3. Good health, Anti-Bacterial Agents, Infectious Diseases, Lung disease, Immunology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.IMM]Life Sciences [q-bio]/Immunology, Transplant patient, medicine.symptom, Legionnaires' Disease, business, TNF-alpha
Abstract: International audience; Legionnaire's disease (LD) is mainly reported in apparently immunocompetent patients. Among them, risk factors include chronic lung disease and smoking. However, LD is also well reported among immunocompromised patients, particularly those treated with anti-tumor necrosis factor alpha therapy, patients with hematological malignancy, and transplant patients. This article discusses the available data on immunity against Legionella spp, epidemiology, clinical presentation, diagnosis, and treatment of LD in immunocompromised patients.
Published: 2017
Full Text: View/download PDF

96. Systemic Candidiasis

Author: Olivier Lortholary, Benoit Pilmis, Zhi-Tao Yang, and Fanny Lanternier
Subjects: business.industry, Immunology, Medicine, Systemic candidiasis, business, medicine.disease
Published: 2017
Full Text: View/download PDF

97. List of Contributors

Author: Fredrick M. Abrahamian, Michael J. Aldape, Edelweiss Aldasoro, Upton D. Allen, Hythem Al-Sum, Milan J. Anadkat, Katherine Anders, Emmanouil Angelakis, Brian John Angus, Anastasia Antoniadou, Fabio Arena, Joop E. Arends, Jose R. Arribas, Andrew W. Artenstein, John C. Atherton, John N. Aucott, Tar-Ching Aw, Hilary M. Babcock, Robin Bailey, Thomas C. Bailey, Adam Z. Banks, David J. Barillo, Ernie-Paul Barrette, Martijn P. Bauer, Roger Bayston, C. Ben Beard, Justin Beardsley, Nick J. Beeching, Rodolfo E. Bégué, Guido Beldi, Constance A. Benson, Elie F. Berbari, Jean-Michel Berenger, Christoph Berger, Jose I. Bernardino, Jacques Bille, Alexander C. Billioux, Ari Bitnun, Iain Blair, Stéphane Blanche, Thomas P. Bleck, Chantal P. Bleeker-Rovers, Gijs Bleijenberg, Karen C. Bloch, Johannes Blum, Emily A. Blumberg, Robert A. Bonomo, Marc J.M. Bonten, Rafik Bourayou, Emilio Bouza, K. Ashley Brandt, Florence Bretelle, Sylvain Brisse, Warwick J. Britton, Itzhak Brook, Matthijs C. Brouwer, Sarah K. Browne, Amy E. Bryant, Silja Bühler, Eileen M. Bulger, R. Mark L. Buller, Leah A. Burke, Christian Burri, Marcus W. Butler, Thierry Calandra, David P. Calfee, Antonia Calvo-Cano, D. William Cameron, Joseph A. Carcillo, Gail Carson, Stephen T. Chambers, Remi N. Charrel, Vinh Chau Van Nguyen, Stéphane Chevaliez, Tom M. Chiller, Eirini Christaki, Kevin K. Chung, David B. Clifford, Nathan Clumeck, Jonathan Cohen, John Collinge, Christopher P. Conlon, Curdin Conrad, Fiona J. Cooke, Jennifer Rittenhouse Cope, G. Ralph Corey, John H. Cross, Burke A. Cunha, Cheston B. Cunha, Benoit D'Journo, George L. Daikos, Johannes M.A. Daniels, Robert N. Davidson, Nicholas P.J. Day, Kevin M. De Cock, Thushan I. de Silva, Henry J.C. de Vries, Stéphane de Wit, Julie Delaloye, David W. Denning, David T. Dennis, Shireesha Dhanireddy, Elodi J. Dielubanza, David J. Diemert, Mehmet Doganay, Tom Doherty, Christiane Dolecek, Arjen M. Dondorp, Abby Douglas, Michel Drancourt, Grégory Dubourg, Michael N. Dudley, Guillaume Durand, Benjamin J. Eckhardt, Androulla Efstratiou, Miquel B. Ekkelenkamp, Ambika Eranki, Hakan Erdem, Gerome V. Escota, Heather L. Evans, Alice Chijioke Eziefula, Florence Fenollar, Alan Fenwick, Joshua Fierer, Roger G. Finch, James M. Fleckenstein, Christina Forstner, Federico Foschi, Pierre-Edouard Fournier, Martyn A. French, Kenneth L. Gage, Lynne S. Garcia, Joaquim Gascon, Arturo S. Gastañaduy, Philippe Gautret, William M. Geisler, Khalil G. Ghanem, Tommaso Giani, Maddalena Giannella, Bruce L. Gilliam, Michel Gilliet, Carol A. Glaser, Youri Glupczynski, John W. Gnann, Ellie J.C. Goldstein, Bruno Gottstein, Frederique Gouriet, Patti E. Gravitt, Michael D. Green, Stephen T. Green, Andreas H. Groll, Roy M. Gulick, Arjun Gupta, Gilbert Habib, Stephan Harbarth, Marianne Harris, Frederick G. Hayden, David J. Hetem, Philip C. Hill, Bernard Hirschel, Aimee C. Hodowanec, Louis Hoffart, Christian Hoffmann, Steven M. Holland, Peter W. Horby, David J. Horne, Sami Hraiech, Mark W. Hull, Angela Huttner, Richard J.M. Ingram, Jasmin Islam, Michael G. Ison, Scott H. James, Claire Jenkins, Stephen G. Jenkins, Jørgen Skov Jensen, Christine Johnston, Theodore B. Jones, Stephen J. Jordan, Kathleen G. Julian, Yasuyuki Kato, Carol A. Kauffman, Keith S. Kaye, Michael P. Keane, James Keeney, Paul Kelly, Stephen J. Kent, Winfried V. Kern, Yoav Keynan, Andrea A. Kim, Isabelle Koné-Paut, Chris Kosmidis, Aloys C.M. Kroes, Frank P. Kroon, Thomas G. Ksiazek, F. Matthew Kuhlmann, Ed J. Kuijper, Jennie H. Kwon, George B. Kyei, Karine Lacombe, Philippe Lagacé-Wiens, Jean-Christophe Lagier, Theresa Lamagni, Luce Landraud, Fanny Lanternier, Kerry L. LaPlante, Stephen D. Lawn, Steven J. Lawrence, Hakan Leblebicioglu, Nelson Lee, James E. Leggett, Philippe Lehours, Pierre-Yves Levy, Rainer G. Leyh, Rebecca A. Lillis, Direk Limmathurotsakul, Jennifer Lin, H.D. Alan Lindquist, Benjamin A. Lipsky, Christina Liscynesky, David Looney, Olivier Lortholary, Franklin D. Lowy, Benjamin J. Luft, Philip A. Mackowiak, Paul A. MacPherson, Valérie Maghraoui-Slim, Patrick W. Mallon, Julie E. Mangino, Oriol Manuel, Oscar Marchetti, Kristen M. Marks, Kieren A. Marr, Jeanne Marrazzo, Jonas Marschall, David H. Martin, Frédéric Matonti, Richard S. Matulewicz, Kenneth H. Mayer, Russell J. McCulloh, Rose McGready, Rennatus Mdodo, Simon Mead, Francis Mégraud, Graeme Meintjes, Sarah C. Metcalf, Marian G. Michaels, Giovanni Battista Migliori, Michael A. Miles, Alastair Miller, Matthew J. Mimiaga, Marie-Paule Mingeot-Leclercq, Elizabeth Ann Misch, Makedonka Mitreva, Julio S.G. Montaner, Caroline B. Moore, Patricia Muñoz, Jose Muñoz, Clinton K. Murray, Didier Musso, Mable Mutengo, Misha M. Mutizwa, Kurt G. Naber, Pavithra Natarajan, Santiago Neme, Paul N. Newton, Ronald A. Nichols, Lindsay E. Nicolle, François Nosten, Luigi D. Notarangelo, Thomas B. Nutman, Paul Nyirjesy, P. Ronan O'Connell, Steven M. Opal, L. Peter Ormerod, Douglas R. Osmon, Marie Boulze Pankert, Giuseppe Pantaleo, Laurent Papazian, Diane M. Parente, Philippe Parola, Shadi Parsaei, Manuel A. Pascual, Rupa Patel, Eleni Patrozou, Jean-Michel Pawlotsky, Sharon J. Peacock, Jean-Claude Pechère, Ivan Pelegrin, Barry S. Peters, Edgar J.G. Peters, Jeannine M. Petersen, Lyle R. Petersen, Vidmantas Petraitis, Luu-Ly Pham, Albert Picado, Adrian Pilatz, Benoit Pilmis, María-Jesús Pinazo, Mathias W. Pletz, Jason M. Pogue, Evelyn L. Polgreen, Philip M. Polgreen, Klara M. Posfay-Barbe, William G. Powderly, Rachel Presti, Guy Prod'hom, Mirja Puolakkainen, Thomas C. Quinn, Didier Raoult, Raymund R. Razonable, Robert C. Read, Robert R. Redfield, Rob J. Rentenaar, Steven J. Reynolds, Camillo Ribi, Malcolm D. Richardson, Michele L. Ritter, Antoine Roch, Jürgen Kurt Rockstroh, Amanda Rojek, José R. Romero, Suzan H.M. Rooijakkers, Daniel Rosenbluth, Sergio D. Rosenzweig, Gian Maria Rossolini, Ethan Rubinstein, Greg Ryan, Steven A. Safren, Vikrant V. Sahasrabuddhe, Pekka A.I. Saikku, Mohammad M. Sajadi, Michelle R. Salvaggio, Carlos A.Q. Santos, Michael J. Satlin, Anthony J. Schaeffer, Christoph Schimmer, Robert T. Schooley, Richard F. Schumacher, Beverly E. Sha, Daniel S. Shapiro, Gerard Sheehan, David M. Shlaes, Shmuel Shoham, Cameron P. Simmons, Dennis W. Simon, Matthew S. Simon, Kari A. Simonsen, Mary P.E. Slack, Tyrel T. Smith, Jack D. Sobel, Maria Souli, Shruti Sridhar, James M. Steckelberg, Dennis L. Stevens, Heather Strah, A. Willem Sturm, Somnuek Sungkanuparph, Sarah J. Tabrizi, Evelina Tacconelli, Chen Sabrina Tan, Randy A. Taplitz, Guillemette Thomas, Lora D. Thomas, Franck Thuny, Guy Thwaites, Frederic Tissot, Tone Tønjum, Francesca J. Torriani, Christian Toso, Paul M. Tulkens, Allan R. Tunkel, Claire E. Turner, Andrew P. Ustianowski, Françoise van Bambeke, Reinout van Crevel, Diederik van de Beek, Christian van Delden, Menno M. van der Eerden, Jos W.M. van der Meer, Tom van der Poll, Jakko van Ingen, Jos van Putten, Bernard P. Vaudaux, Sten H. Vermund, Raphael P. Viscidi, Kumar Visvanathan, Govinda S. Visvesvara, Lorenz von Seidlein, Florian M.E. Wagenlehner, Anna Wald, Thomas J. Walsh, David C. Warhurst, David W. Warnock, David A. Warrell, Mary J. Warrell, Adilia Warris, Richard R. Watkins, David J. Weatherall, Rainer Weber, Wolfgang Weidner, Jonathan R. White, Peter J. White, James Whitehorn, Richard J. Whitley, Christopher J.M. Whitty, Willem Joost Wiersinga, Mark H. Wilcox, Thomas N. Williams, Cara C. Wilson, Mary Elizabeth Wilson, Hilmar Wisplinghoff, Robin Wood, Richard G. Wunderink, David Wyles, Zhi-Tao Yang, Jonathan S. Yoder, Najam A. Zaidi, Andrea J. Zimmer, Jane N. Zuckerman, and Alimuddin Zumla
Published: 2017
Full Text: View/download PDF

98. Clinical features and prognostic factors of listeriosis: the MONALISA national prospective cohort study

Author: Odile Fremin-Batteux, Juliette Clarissou-Philippe, Benoît Jauhlac, Severine Guyetand, Jacques Gasnault, Corinne Haioun, Liamine Aissaoui, Marie-Christine Pages, Marie-Pierre Fos, Christian Rose, Didier Hubert, Marie-Rose Rothe, N. Bouziges, Benoît Huc, François Devianne, Sabine Bidart, Anne Forest, Kevin Bertrand, Mohamed Eldeghedy, Annick Verhaeghe, Caroline Malderet, Anne Bertrou, Bernard Guerquin, Catherine Duche, Muriel Archambaud, Rabea Cotteret, Olivier Toullalan, Yves Devaux, Smail Bergheul, Valérie Sivadon-Tardy, Pierre-Gilles Merville, Geneviève Blanchard-Marche, Didier Raoult, Bernard Hory, Florence Richardin, Evelyne Belle, Mohamed Menouar, K Guitteaud, Mohamad Mohty, Ambroise Montcriol, Max Laurin, Aurélia Picard, Jean-Paul Mira, Marie-Charlotte Chopin, Richard Bonnet, Michel Wolff, Sébastien Maillez, Jeanne Maugein, Véronique Leblond, Nicola Walid, Bernard Gauche, Mathieu Evillard, Hassen Jeddi, Anne Bourlet, Isabelle Grawey, Thierry Jault, Sandrine Hiret, Valerie Gaborieau, Véronique Boin-Gay, An Kim, Thierry Constans, Jean-François Gaide, Martine Giraud, Eric Meaudre Desgouttes, Alain Fur, Abdallah Maakaroun, Olivier Matray, Bertrand Maubert, Frédérique Péchinot, Aurelie Garbi, Claire Delbrouck, Benoît Grandclerc, Vincent Cadiergue, Hervé Lécuyer, Bernadette Grignon, Thierry Bensaid, Nicole Constantin, Yannick Chevalier, Hassène Rahmani, Thierry Levent, Joelle Desliers, Florence Van de Velde, Xavier Adhoute, Clara Andriau, Christophe Charasse, Rémi Vatan, Benoît Martha, Alain Lecis, Didier Albert, Romain Jacobs, Hélène Lefranc, Christian Martin, Nasseur Rezgui, Bertrand Pigeon, Catherine Le Henaff, Dominique Cassignard, Françoise Cotes, Eric Pujade Lauraine, Jean-François Gattault, Nicole Ferreira-Maident, Noémie Jourde-Chiche, Hélène Garrec, Olivier Darchen, Carole Schwebel, Marie-Christine Bezian, Patrick Daoud, Tsouria Becaid, Simone Laluque, David Broche, Christine Boisselier, Pascale Martres, Sarah Hammami, Brigitte Olivier, Jean-Marie Nkunzimana, Eric Monlun, Isabelle Marterl-Lafay, Marion Carboni, Marie-Françoise Mattei, Sandrine Castelin, Isabelle Barillot, Marie-Noelle Cufi, Thomas Kaiser, Catherine Herry, Pascal Hutin, Jean-Pierre Bronowicki, Bernard Branger, Pierre Thomas, Elie Zagdoun, Anne Goquelin, Ziad Assaf, Ingrid Croquet, Bruno Pozzetto, Thomas Similowski, Anne-Isabelle Briere, Marie-Thérèse Albertini, Mariam Blaka, Christelle Tassot, Anne Gaschet, Jean-Philippe Lavigne, Antoine Pujol, Philippe Colombat, Edouard Devaud, Hana Talabani-Boizot, François Barière, Anne-Marie Cordier, Philippe Gueudet, Georges Simon, Anne-Sophie Lipovac, Françoise Bandaly, Anne Beauplet-Lepage, Sylvie Prince, Charlotte Jouzel, Jean-Luc Deboutin, Patrick Zavadil, Louis Puybasset, Marie-Cécile Petit, Loïc Guillevin, Kamel Touati, Christophe Ntalu Nkato, Sylvie Carette, Jacques Vaucel, Chantal Delasalle, Marine Gross Goupil, Laurent Gutmann, Christiane Payen, Annick Barboteau, Firouzé Bani-Sadr, Christophe Legendre, Philippe Roulier, Elie Azria, Ibrahim Farah, Isabelle Rouquette-Vincent, Anne-Sophie Erena-Penet, Philippe Labadie, Eric Josien, Aicha Derragui, Mathieu Legrand, Odile Beyne-Rauzy, Jean-Marc Nabholtz, Marie-Joelle Demarcq, Olivier Garosi, Michel Deiber, Fabrice Chaix, Bertrand Souweine, Anne Collignon, Gisèle Renard, Mickael Jego, Gilles Bernardin, Anne Allart, Jocelyn Barrier, Marc Vasse, Philippe Ménager, Marc Wurmser, Abderkader Ouazir, Olivier Gontieron, Yvon Berland, Sébastien Trouiller, David Leysenne, Christophe Ozanon, Fanny Autret, Tahar Saghi, Loïc Dopeux, Sophie Benoit-Coustou, T. Fraisse, Christine Maillard, Karine Nikodijevic, Georges Kaltenbach, Angéline Jamet, Philippe Aucher, Julie Bottero, Marie-Claude Piffaut, Marianne Besnard, Florence Courillon, Marie Bonfils, Christine Ghevaert, Marie Destors, Eliette Jeanmaire, Franck Zerbib, Manuel-Luis Gameiro, T Prazuck, Laurent Mandin, Olivier Guisset, Marguerite Fines, Toufik Feddal, Agnès Jouffret, Louis Mesnard, Thomas Bourrée, Hasinrina Razafimahefa, Sylvestre Tigaud, Vincent Estève, Philippe Malherbe, Jean-Michel Salord, Pascal Adam, Bertrand Rozec, Michel Fuillet, Olivier Lemenand, Denis Quinsat, Ana Danalaché, Véronique Vialette, François Brosset, Patrick Messner Pellenc, Nicolas Heisel, Edouard Girard, Régine Martin, Olivier Garesslin, Catherine Mille, Alexandre Gascon, Marc Nicolino, Laurence Mouly, Claire Fabre, Bénédicte Ponceau, Marie-Etiennette Emeriau, Pascal Cathebras, Bérangère Bernardaud, Michèle Pérouse de Montclos, O. Arsene, Karine Grenet, Yazdan Yazdanpanah, Sten De Witte, Anne Scemla, Laurence Bouillet, Christophe Burucoa, Vincent Loffeier, Séverine Visentin, Luc Desfrere, Miloud Arabi, Frédérique Costa, Sylvie Lechat, Ali Chekroun, Raymond Ruimy, Marie, Jérôme Bizet, Xavier Nassif, Baihas Dib, Patrick Bert-Marcaz, Laurent Martin Lefèvre, Nicholas Sedillot, Blandine Cattier, Emilie Boidin, Daniel Sondag, Aude Bourrouillou, Alain Noirot, Franck Desemerie, Fréderic Heluwaert, Catherine Tamalet, Marc G. Berger, Jean-Daniel Lelièvre, Dominique Perotin, Abdelkader Bemrah, Alain Lozniewski, Bernard Borstein, Hanna Eid, Diana Suatean, Virginie Mignaut, Jean-Claude N'guyen, Valérie Le Goff, Laurent Teillet, Christophe Rolland, Gwenaël Alfonsi, Florence Lachenal, Philippe Bossi, Yves Botreau, Florence Doucet Populaire, Henry Jardel, Nicolas Gallo, Elias Jabre-Sikias, Michel Dupon, Hélène Brihier, Isabelle Patry, Alexandre Leclercq, Bernadette Tourrand, Christophe Roussel, Jean-Emmanuel Kurtz, Bénédicte Paindaveine, Simon Elhadad, Richard Sanchez, Eric Sgro, Pierre Berger, Valérie Murbach, Anne Holstein, Florence Martin, Taoufik Merabet, Amélie Benbara, Milagros Ferreyra, Laure Esposito, Pierre Delobel, Antoine Andremont, Marc Bourlière, Carole-Anne Boudy, Jean-Baptiste Gaulthier, Laurent Tacchini, Olivier Marpeau, Sonia Tesseydre, Marie-Pierre Coulhon, Nathalie Hodee, Marie-Chrsitine Conroy, Pierre Weinbreck, Roland Leclercq, Laurent Souply, Christian Bidault, Annie Elbez, Marie-Annick Lebreton, Patrick Brisou, Agnès Ferroni, Jean-Louis Pourriat, Nadia Anguel, Christian Noel, Philippe Jouvencel, Eric Pichard, Xavier Martin, Mathieu Coste, Pierre Zuber, Catherine Neuwirth, Jean-Pierre Hacot, Paul Aye, Jérôme Guinard, Yves Pean, Jean-Christophe Dengo, Fabrice Petassou, Didier Viole, Thierry Messiaen, Jean Beytout, Philippe Petitjean, Ferdinand Savare, Patrice Cuvillier, Sophie Coignard, Hélène Anglaret, Nassim Kamar, Elisabeth Chachaty, Karine Guimard, Louis Braem, Hacene Fezoui, Pierre Martin, Jean-Paul Viard, Claire Larroche, Nicolai Claudiu-Plesa, Thierry Benoit-Cattin, Olivier Moquet, Thierry Pasdeloup, David Rosay, Rodolphe Jean, Jean-Bernard Mariette, Marc Debouverie, Hervé Peltier, Mustapha Terki, Jacques Daleas, Valérie Dattin-Dorrière, Michel Vergnaud, Emmanuel Grimprel, Sylvène Rosselli, Jean-Marc Didier, Pierre Faurie, Luc Frimat, Aziza Mandjee, Sabine Etchemendy, Pierre Tissières, Jean Nakhleh, Sylvie Mariette, Christian Perronne, Bruno Carbonne, Nathalie Houssiaux-Maisonneuve, Tristan Ferry, S Beague, Anthony Sebban, Marie-Thérèse Hili, Jean-Michel De Kermadec, Lucien Brasme, Gilles Blaison, Caroline Garandeau, Jean-Pierre Sollet, Laurent Tronchon, Thierry Samson, Julien Gesquière, Nicolas Ettahar, Alain Créange, Etienne Laurens, Véronique Equy, Fréderic Bart, Bernard Bouffandeau, Christine Vaillant, Valerie Pesque, Jean-Marc Lalot, Marc Levy, Michel Kaidomar, Mihaela Saplacan, Sterenn Yvenou, Marie-Isabelle Steibach, Emmanuelle Cambau, Agnès Riche, François Fourrier, François Raffi, Mélissa Lalu, Henri Bérard, Danielle Clave, Jean-Claude Mouries, Martine Porcheron, Jean Cabalion, Richard Lamarca, Nathalie Canu, Jean-Baptiste Roseau, Annabelle Stoclin, Luca Luminitan Elena Lupean, Rémi Gebeile, Celia Salanoubat, Carole Marmouset, Pierre Bigot, Anne-Laure Breton, Pierre Kalfon, Colette Vincent, Sophie Marty, Olivier Tandonnet, Alexis Redor, Xavier Valette, Ourida Aoudia, Jacques-Arnaud Seyrig, Bertrand Beaune, Hugues Aumaitre, Georges Pinon, Yann Leveneur, Sylvie Charachon, Raoul Herbrecht, Henriette de Valk, Gary David, Julien Pouyanne, Marc Dommergues, Majed Al Chaar, Véronique Blanc-Amrane, Pascale Guillarmé Grossmann, Bruno Abraham, Yves Morel, Philippe Suel, Denis Sautereau, Olivier Guilloy, Tu Anh Tran, Frédéric Laurent, Zahir Amoura, Jacques-Olivier Bay, Zoubida Elharie-Heraux, Joyce Sibony-Prat, Bernard Guillois, Dominique Rohmer-Heitz, Audrey Barrelet, Jérémie Courouble, Jean-Paul Herry, Daniel Vittecoq, Annie Vermesch-Langlin, Jean Auroux, Claude Aubert, Thierry Harvey, Ghislaine Lamoine-Gimet, J. Riahi, Florence Soraudeau, Bachar Al-Jalaby, Caroline Périsson, Khélifa Ayouz, Florence Cardot, François Maillet, Alain Goux, Théophile Magna, Bertille de Barbeyrac, Adrien May, Dominique Andreotti, Olivier Jonquet, Hélène Dumouchel, Didier Thibaud, Philippe Morlat, Pascal Chevalet, Pascal Ancelin, Guy Chambreuil, Cécile Le Boterff, Anne Ceriez, Olivier Detante, B Pangon, Claude C.A. Bernard, Vincent Cailleaux Pierre-Etienne Cailleux, Jordi Miatello, Pierre-Yves le Berruyer, Sylvain Kouaho, Michel Briaud, Hélène Delaby, Patrick Herbecq, Christine Segonds, Véronique Jault, Pascale Brunel, Christine Dussopt, Jean Thore, Jean-Marc Thouret, Jean-Marc Kerleau, François Le Baron, Slavius Matica, Sophie Leautez-Nainville, Matthieu Pecquet, Laurent Bret, Yacine Sedjelmaci, Pierre Metton, Habiboulaye Diallo, Jany Rey Zermati, Arnaud Delahaye, Hélène Chaussade, Laurent Mandelbrot, Emilie Bessede, Olivier Casanovas, Paul Pierrot, Annick Legras, Dominique Lauque, Hélène Gatti, Jean Catineau, Ebutu Likose, Gilles Capellier, Eric Kibbrecht, Freddy Thibaut, Patrick Valadier, Chantal Lemble, Joël Gaudelus, Joelle Mellier, Joëlle Brochen, Emmanuel Gascou, Stéphane Bonacorsi, Stéphanie Bannier, Bruno Fantin, Didier Raffenot, Valérie Revel, Hakim Amroun, Huguette Negrery, Anne-Laure Fauchais, Paul Mercury, Michel Chuzeville, Christian Zumbo, Nicolas Després, Pascal Roblot, Jérôme Pasche, Jean Claude Boufetteau, Jocelyne Caillon, Julien Boileau, V. Rabier, Benjamin Manéglier, Emilie Jourdes, Franck Ceppa, Christine Recule, Nicolas Degand, Benoît Henry, Thierry Baranger, Dominique Pateron, Agnès Pélaquier, Gérard Bouchet, Hélène Fiette, Ozel Guiden, Dana Ranta, Etienne Ruppé, Nabil Chiouk, Jacques Breuil, Dominique Leduc, Véronique Loustaud, Hervé Métenier, Michel Durand, Isabelle Mahé, Leila Karaoui, Marie-José Collus, Mehran Monchi, Olivier Belmonte, Romain Blondet, Jacques Thierry, Karine Humbert, Gilles Salama, Marie-Noelle Heurtaux, Cécile Goujard, Bruno Sivery, Martial Boisseau, Redouane Dahoumane, Pierre Delour, Christian Niels Meyer, Anne Faudon-Gibelin, Gérard Poulain, Roger-Charles Luciani, J.-C. Souquet, Olivier Grossi, François Vandenesch, Sylvain Mermont, Jacques Bronner, Sonia Dahan, Paul Marzouk, Pascal Pouedras, Noureddine Djafari, François-Xavier Caroli-Bosc, Jean-François Dessin, Brigitte Gruffat, Armelle Morin-Fatome, Sylvie Thoinet, Bano Konate, Jean-Winoc Decousser, Claire Poyart, Patrick Plessis, Olivier Millet, Vincent Cattoir, Françoise Geffroy, Manica Vasseur, Pierre Carli, Isabelle Citony, Christian Richard, Nicolas Sigur, Patrick Marthelet, Luwawu Mbimba, Pierre Feugier, Philippe Sauder, Hama Djerad, Evelyne Bourgerette, Hanen Chahtour, Adrien Lemaignen, Dominique Bechade, Patrick Ochocki, Antoine Vieillard Baron, Dominique Astruc, Marie-Pierre Moiton, Nicolas Dubois, Sylvie Ledru, Corinne Seknazi, Hélène Poupet, Jean-Philippe Brieux, Gérard Barthélémy, Aihem Yehia, Louis-Jean Couderc, Ahmed, Françoise Rigaux, Yohann N'guyen, Philippe Bethery, Damien Corberand, Etienne Auvray, Paul-Louis Woerther, Christian Combe, Sophie Delesalle, Jean-Marie Piala, Faraj Al Freijat, Philippe Juvin, Malcolm Lemyze, Hyacine Rey, Claire Larible, Noel Milpied, Lémia Zgarni, Julia Gaillard, Agnès Juven, Paola Otean, Adrien Melis, André Pechinot, Olivier Bouchaud, Olivier Chassin, Pierre Hausfater, Asma Trabelsi-Jnifen, Vincent Grobost, Didier Lemery, Pierre Soury, Françoise Brevet, Jacques Tankovic, Dominique Sansot, Jean Louis Salomon, Charlotte Cordonnier, Brigitte Lamy, Antoine Maisonneuve, Dominique Pressac, Claude Rémy, Rodolphe Sobesky, Stéphanie Cognet, Pierre Cougoul, Didier Jan, Dominique Perrotin, Cécile Hombrouk-Alet, Thierry André, Gilbert Pochmalicki, Serge Girard, Vincent Zerr, Guillaume Cadiot, Claudine Lasbasses, Michel Slama, Abderrazak El Yamani, Sophie Brovedani, Jean Armengaud, Romain Hernu, Géraldine Mascade, Aurélien Lorléa'ch, Ali Akkari, Mathieu Tourdjman, Christopher Payan, Eric Jullian, Nathalie Fonsale, Frédéric Riehl, Paul Strock, Geneviève Grise, Philippe Mottaz, Christian Floriot, Marie-Noëlle Ungeheuer, Denis Caillot, Arnaud Chalvon-Demersay, Catherine Branger, Stanislas Bruley des Varannes, Marc Paccalin, Marie-Pierre Danjean, Alexandre Mebazaa, Xavier Brunet, Roland De Varax, Laurence Delhoustal, Sophie Haro, Bruno Chabanon-Pouget, Isabelle Goidin, Dominique Chudersky, Corinne Costes, Delphine Chatellier, Maud Gelez, Damien Dassant, Pascal Joly, Jean-Michel Arnal, Zakaria Hamitou, Philippe Rondepierre, Carole Pignon, Valérie Crombe, Amanda Lopes, Chrystelle Kemenar, Olivia Raulin, Anne-Cécile Hochart, Sandrine Gérart Pons, Valérie Zeller, Guillermo Reyes Ortega, Mathilde Guérin, Audrey Migraine Bouvagnet, Florence Eboue, Isabelle Loury-Lariviere, Sophie Leotard, Suzanne Lima, Marie Kassis, Jean-Luc Donay, Jean-Pierre Audié, Guillaume Cartron, Arnaud Ribier, Fanny Buron, Mirela Tuca, Marius Semenescu, Arnaud Serre, Vincent Quentin, Denise Bouyssou-Destriau, Violaine Bresson, Christine Chandesris Joséphine Chapalain-Cagnon, Eric Cua, Henri Courtade, François Bénézit, Sébastien Lamache, Philippe Bonnefoy, Francis Schneider, Richard Monarchi, Adeline Schendel, Paramasiven Mootien, Ghislaine Gardes, Pierre-François Westeel, Jean-François Magny, François Caron, Jocelyn Michon, Didier Eyer, Isabelle Ronda, Pierre-Yves Robillard, Frédéric Renou, Anna Faucher, Jean-Robert Harlé, Anne Debernardi, Grégory Akerman, Benoît Fontenel, Pierre Hourdebaigt-Larrusse, Marie-Noëlle Adam, Aude Lessene, Abdelkader Hrichi, François Blot, Athéna Le Pierres, Romain Lemarie, Françoise Granier, Véronique Tardy, Marc Gatfosse, Pierre-Marie Roger, François Goupil, Saïd Aberrane, Franck Bernardi, Isabelle Plantier, Nathalie Funakoshi, Jean-Gilles Delecalle, Patricia Barbut, Jacques Reynes, Christophe Roy, Sophie Perreve, Michel Garre, David Ribes, Cyrille Ede, Jean-Claude Dausset, Francis Duchene, Jean Caussin, Michelle Becker-Schneider, Gilles Berthelot, Damien Dupont, Jean-Michel Gillot, Aurélie Messager, Jean-Marie Pannecouck, Jean-Christian Roussel, Alain Reynaud, Sylvie Cariou, Anne Dao, François Guillemot, Martin Martinot, Patrick Casali, Anne-Sophie Poirier, Aissa Kerchache, Necera Sakek, Eric Porthault, Christophe Decoene, Chantal Ache-Papillon, Brigitte Bicais, Jean-Claude Feugier, Thierry Masseron, Charles Marty-Ane, Daniele Goldgran Toledano, Jean-Christophe Dubus, Damien du Cheyron, Dominique Decré, Jean-Loup Pennaforte, Ahmed Tigaizin, Bernard Vache, Eric Oswald, Claire Moulinoux, Anne-Christine Jaouen, Caroline Charlier, Anne-Laure Virlouvet, Ali Kara, Jean-Luc Sicsic, Sylvie Goffart, Mathieu Zuber, Claudine Fèbre, Olivier Lortholary, Mathieu Dupont, Annie Vessieres, Thierry Helvadjian, Thomas Signouret, Cedric Daupin, Sandrine Essouri, Jean-Louis Jacob, Pascal Boileau, Caroline Blazejewski, Quentin Lepiller, Juan-Pablo Maureira, Eddy Lebas, Christophe Deschamps, Amévi Ananivi, Clovis Foguem, Daniel Adoue, Abdourahim Chamouine, Alain Michault, Bruno Guérin, Olivier Baud, Clara Vinci, Thierry Weitten, Jean-Marc Eychene, Marie Froidure, Julien Obiols, Patricia Roussellier, Marc Lecuit, André Cabié, Saskia Foucart, Karim Belhadj, Michel Cingotti, Bruno Dumoulard, Jean Puyhardy, Etienne Danquechin Dorval, Lucile Mendes-Martin, Enrique Casalino, Luc Jarrige, Fabien Lambiotte, Philippe Masson, Mohammed Mansouria, Pierre Thouvenot, Katy Jeannot, Martine Nyunga, Valérie Macci, Florent Masia, Claire Briere de la Hosseraye, Wassila Anteur, André Sommabère, Marie-Claude Germain, Isabelle Arnault, Bernard Carbonelle, Philippe Devos, Daniel Protar, Tiphaine Gaillart, Ludovic Lassel, Laurence Hamou-Plotkine, David Trystram, Thierry Bureau, Olivier Collard, Fanny Vuotto, Sophie Malhiere, Frederique Canis, Gillles Plainfosse, Catherine Lechiche, Bertrand Lassere, Martine Chouraqui, Jean Baptiste Michot, Fethi Radhouane-Khanjari, Carole Barbier, Pascal Bonitchi, Abdelaziz Benkhelil, Odile Salmon, Laurent Damaj Gandhi, Bertrand Minguet, Michel Wagner, Odile Falguières, Zahr-Eddine Ali Chaouche, Eric Zaoui, Isabelle Guichard, Bernard Huttin, Apollinaire Karirisi, Gaël Cinquetti, Christophe Plane, Lionel Rostaing, Yanne Henry-Andrieu, Daniel Re, Virginie Verrier, Pascal Bolot, Michel De Biasi, Laurence Vrigneaud, Mathilde Turpin, Marie-Claude Demachy, Etienne Roussel, Michèle Blancs, Olivier Join-Lambert, Yves Ville, Thierry Granger, Gilles Hilbert, Virginie Medeau, Daniel Villers, Benoit Pilmis, François Gouraud, Emmanuel Ardiet, Catherine Heyraud-Blanchet, Alain Devidas, Hélène Dieye, Julie Cremniter, Jean-François Bergmann, Rozenn Le Berre, Virginie Leguen, Daniel Royer, Gilles Le Maout, Christian Harou, Sylvie Gabriel-Soléan, Yves Regouby, Martine Pestel-Caron, Patrick Brunet, David Boutoille, Emmanuelle Bonnin, Patrice Coulon, Marc Sullice, Marianne Barbieux, Gilles Cambonie, Joëlle Tricoire, Marie-Nadège Bachelier, Delphine Briend, Céline Ramanantsoa, Nathalie Bednarek, Didier Lebreton, Julien Lagrandeur, Damien M'Bey, Philippe Audeguy, Elie Saliba, Lena Damaj, Hassan Fallouh, Pascal Couturier, Fabrice Prévost, Yves Domart, Marie-Odile Lafforgue, Anne Le Du, C. Beuscart, Pierre Guillet, Fabrice Larrazet, Marie-Hélène Hausermann, Henri Robert, Nicolas Fanjaud, François Goehringer, Thomas Bachelot Philippe Badia, Jean-Michel Coulaud, Cristel Fissore Magdelein, Renaud Defebvre, Anne-Sohie Moreau, Johan Courjon, Gilles Salles, Michel Mialon, Silvia Iacobelli, Emmanuelle Bille, Marie-Christine Barbier, Yves Aubard, Patrice Badila, Jean-Philippe Rasigade, Alban Deroux, Evelyne Lecaillon-Thibon, Michel Godin, Abdelmajid Djeffal, Viorica Badurescu, Meriem Canitrot, Pierre Blanchard, Antoine Legros, Laurence Got, Françoise Duluc, Mylène M. Maury, Gilles Dassieu, Nordine Khodeir, Jean-Marie Duez, Mathieu Morincomme, Jérôme Lacroix, Mathieu Revest, Koffi Blewoussi, Isabelle Barazer, Françoise Poitevin, Camille Seignovert, Stéphanie Honore Bouakline, Anne Heidt, Brigitte Malbruny, Julien Desblache, Christian Cattoen, Eric Jaunait, Bruno Chaminade, Claude Bazin, Jonathan Chelly, Anne Pottier, Alain Schmitt, Alain Tissot, Karim Dadoun, P. Rebattu, Claudine Contamin, Arnaud Guerard, Nathalie Ravet, Sandrine Khalifa-Thellier, Marlène Chatron, Gaëlle Dörr, Hélène Biessy, Emmanuel Forestier, Bruno Devaux, Jean-Jacques Grelaud, Xavier Tchenio, Marie-Cécile Ploy, Jérémie Violette, Michèle Burdin, Lionel Falchero, Dominique Jacomy, Jean-Christophe Rozé, Damien Labarriere, Stéphane Leroux, Corinne Meregnani, Assia Ferhat Carre, Paul Orode, Jean-Gabriel Paul, Catherine Godon, Agnès Vinay, Régine Barraduc, Dominique Dallay, Alexandre Ampère, Anne-Gaelle Kervegant, Guillaume Louart, Dominique Beal Ardisson, Francoise Leonetti, Jean-Yves Baril, Stéphanie Haiat, Bincy Darre, Jérôme Bay, Yvan Gauthier, Sylvie Radenne, Pierre-Yves Gueugniaud, Philippe Ravaud, Luc Landraud, Guillaume Ranchon, Loïc Chimot, Véronique Duval, Ilhem Agha-Mir, Sabine Camiade, Estelle Wafo, Jean-Patrick Laporte, Mariam Roncato-Saberane, Camille Bron, Patrice Laudat, Samir Kennouche, Nawel Afroukh, Dominique Neri, Hakim Kherouf, Yoar Hichri, Pierre-Edouard Bollaert, Gwenaelle Vary, Denis Castaing, Christine Lefort, Sébastien Rouget, René Robert, Christelle Guillet-Caruba, Catherine Simonin, Alain Vighetto, Severine Cabasson, Alain Brusset, Alexandra Doloy, Christel Cherlet, Ahmed Rouidi, Marina Salvucci, Réginald Pordes, René-Gilles Patrigeon, Emmanuelle Dupre-Narlet, Jacques Minet, Fethi Taleb, Anne-Marie Colingorski, Tahar Hadou, Sylvain Diamantis, Isabelle Glorieux, Thierry May, Jean-Claude Colombani, Anne Berth-Farges, Nicole Desplaces, Renaud de Tayrac, Elisabeth Walter, Fabienne Lorge, Pascal Reboul, Nathalie Dournon, Laurence Estépa, Marie-Lina Toubia, Mathilde Flahault, Thierry Delacour, Dominique Hurel, Hélinoro Andriamaneo, Cécile Bébéar, Denis Grasset, Miloud Serier, Oléna Orléva, Nadine Dubroca, Hervé Gentilhomme, Jean-Luc Baudel, Isabelle Lavenu, Salim Smati, Carlo Saroufim, Eric Placidi, Albert Sotto, Benoît Libeau, Hélène Leroy, François Golfier, Christophe Dollon, Laurence Desnoulez, Eric Barre, Daniel Cohen, Pascal Priollet, Thierry Marsepoil, Benoît Lionnet, Jacques Tebib, Pascale Penn, Antoine Bouissou, Christian Roth, Olivier Martinet, Anna Schmitt, Nathalie Fruleux, Fouzia Radaoui, Jean-Marc Lessinger, Virginie Morando, Jean-Jacques Maillet, Christophe Fruchart, François Boué, François Goffinet, Franck Lellouche, Martin Demarchi, Alain Geissler, Jean-Charles Picaud, David Assouline, Patricia Brazille, Philippe Guimier, Marie-Françoise Dabysing, Bruno Delpeuch, Vanessa Tran, Guy Gengembre, Delphine Deligne, Dominique Vodovar, Yvan Touze, Sabrina Parent, Anne Decoster, Camille Dewitte, Emmanuel Weiss, Thierry Lambert, Thomas Guimard, Vincent Caille, Claude Guérin, Françoise Evreux, Geneviève Barjon, Basile Ondze, Damien Fournier, Olivia Bandin, Sophie Mignart, Henri Demontclos, Didier Perez, Jacques Croize, Nicole Desbois-Nogard, Guenièvre Imbert, Clarisse Dupin, Khalid Ridah, Marie-Christine Varin, Guillaume Arlet, Edith De Clareuil, Marie-Line Eustache, Patricia Le Pimpec, Louise Fortin, Eugène Ngami, Fabrice Mihout, Cecilia Esnault, Vincent Bouden, Véronique Annaix, Yves Poinsignon, Aurélien Lorchleac'h, Jean-Marc Degreff, Marie Garofano, Renaud Mesnage, Anne-Marie Roque-Afonso, Alain Chevailler, Stéphane Hominal, Thierry Charbonnier, Adrianna Bildea, Fabien Fily, Benjamin Davido, Emmanuel Rassiat, Assi Assi, Stéphanie Brunet, Hervé Jacquier, Catherine Claise, Annie Durand, Yannick Monceau, Pierre Blanc, Jean-Marie Sire, Yves Sucin, Jean-Pierre Zarski, Nathalie Bronet, Ingrid Lafon, Philippe Rey, Jacques Markarian, Eric Sennevile, Olivier Wink, Guilène Barnaud, Anne-Sophie Peultier, Sabine Taylor, Rim Savatier, Patrick Valayer, Claude Negrier, Selim Jennane, Edouard Begon, Laura Hyerle, Delphine Bridoux, Claire Daurel, Benoît Dalle, Mathilde Lescat, Philippe Stolidi, Elodie Perrodeau, Xavier Heches, Pierre Castelnau, Philippe Bray, Jean-Claude Texier, Serge Rossignol, Maud Brung-Lefebvre, Jean-François Subra, Jean-Marie Delarbre, Morgane Schneerson, Guyro Jang, Mona Mehri, Nathalie Landgraf, Pierre-Marie Girard, Armand Goll, Zaineb Bekguesmia, Christophe Clement, Michel Collet, Vincent Maze, Amine Benjelloul, Solène Durliat-Ellie, Vincent Letouzey, François Schmitt, Valérie Martinez, Sarah Watson, Abderrezak Bouasria, François Barbier, Raphael Lauretta, Mirana Razafimahery, Cristina Sirbu, Patrick Malherbe, Anne Wuillai, Ludovic Lesecq, Philippe Gaudard, Serge Houssaye, Jacques Monsegu, Gilles Rival, Chantal Chaplain, Jean-Didier Grangé, Oana Zamfiri, Florence Nguyen-Khac, Marc Portneuf, Jean-Michel Pawlotsky, Delphine Bonnet, Laurent Traissac, Sophie Hamon-Charles, Didier Dreyfuss, Louis Bernard, Laurence Detourmignies, Olivier Martineau, François Pettinelli, Marc Zandecki, Michel Dreyfus, Alain Chapelle, Sébastien Sabbat, Anne-Sophie Labussiere, Jean-Louis Gaillard, Chloé Plouzeau-Jayle, Patrick Zoveda, Véronique Leflon, Marie Levy, Aurélie Labé, Bruno Soulie, Raoul Jacques Bensaude, Hecham Moussa, Sylviane Catteu, Nathalie Biron, Loïc Masson, Georges Mourad, Nejla Aissa, Dragos Ciocan, Hubert De Boysson, Jean-Luc Bouyer, Patrick Yeni, Thierry-Pascal Zame, Caroline Thomas, François Cavalié, Laurence Koulmann, Christophe Rioux, Olivier Barraud, François Bricaire, Marguerite Le Poulain, Marie-Noelle Noulard, René Thomas, Guy Semet, Laurent Mosser, Olivier Marret, Brigitte Rivière, Vincent Jarlier, Jean-Philippe Coindre, Marc Villemain, Martin Pierre, Yacine Benkaci, Philippe Chiron, Hoang Vu-Thien, Jérôme Gournay, Andrea Labaune-Kiss, Brigitte Lauzanne, Fanny Lemercier, Souad Silhadi, Imad Kansau, Christophe Poncelet, Olivier Baldesi, Francis Thuet, Olivier Leroy, Aurore Lamberet, Camille Petit Hoang, Sophie Micheli, Ayman Abokasem, Hakima Nesrine, Pierre Lureau, Christian Chidiac, Vincent Piriou, Fabien Zoulim, Dieudonné Nicobaharaye, Anne Tixier, Isabelle Matheron, Soumeth Abasse, Victoria Cacheux, Serge Herson, Christine Fuhrmann, Olivier Proost, Bernard Bedock, Olivier Rogeaux, Mostapha Hajjar, Anne Reverseau, René Courcol, Françoise Carmagnol, Yves Guénard, Céline Ménard, Bouchra Lamia, Bruno Lemmens, Damien Bouhour, L. Lequen, Gaëlle Baty, Cédric Bouet, Dominique Guerrot, Stéphane Blanc, Catherine Chirouze, Anne-Hélène Reboux, A. Vachée, Gregory Taurin, Myriam Mein-Bottini, Jean-Pierre Belot, Alain Lafeuillade, Patricia Gabez-Therou, Philippe Labrousse, Bernard Jarrousse, Philippe Noto, Vincent Brunot, Philippe Condominas, Marion Challier, Béatrice Berçot, Delphine Anuset, Mélanie Daval Cote, François Bernasconi, Y. Costa, Chandrah Goburdhun, Bernard Gressier, Alban Michaud-herbst, Franck Charlier, Moussa Hecham, Luc Boulain, Hélène Corneloup, Alix Greder Belan, Nicolas Boussekey, Claire-Antoinette Dupuy, Yannick Rouquet, Benoit Renard, Benifla Jl, Etienne Javouhey, Michèle Granier, Marie-Christine Jaffarbandjee, Emilie Piet, Benoît Bergues, Claire Malbrunot, Catherine Tiry, Philippe Mérigot, Mouna Ben Soltana, Chantal Roure Sobas, Florian Radenac, Yves Thomas, Agathe Blaise, Sylvie De Martino, Laurence Legout, Gabriel Choukroun, Jean-François Muir, Peggy Dupretz, Patrick Dupont, François Guichart, Julie Jean, Jean-Michel Descamps, Bernard Kittschke, Anne Gruson, Gerard Viquesnel, Marie Keller, Pascal Chavanet, Françis Vallet, Yvan Vaschalde, Jean-Luc Hanouz, Gerard Lina, Françoise Loison, Simon Vincent, Jean-Paul Thellier, Moncef Afi, Dominique Zagozda, Hélène Sokeng-Affoule, Marc Le Bideau, Jean-François Loriferne, Alain Gravet, Sophie Deprecq, Tarik Naceur, Severine Mielczarek, René-Jean Bensadoun, Bernard Karkous, Yves Bléher, Jocelyne Poulain, Véronique Goulet, Laurence Nicolet, Sophie Arista, Antônio Lúcio Teixeira, Jean-François Schved, Laurent Nicolet, Claire Lecomte, Faiza Benddif-Fin, Michel Aumersier, Laurence Burc-Struxiano, Maxime Thouvenin, Samia Harbi, Mathieu Detave, Catherine Rebeyrotte, Jean-Paul Kisterman, Bruno Berdin, Pascal Vincent, Laurent Argaud, Elisabeth Parisi-Duchene, Geneviève Julienne, Fernanda Farto-Bensasson, Georges-Fabrice Blum, Sad Gaizi, Tali-Anne Szwebel, Raphaël Lepeule, Marie-Thérèse Climas, Anne-Françoise Dillies, Amar Boudhane, Umberto Simeoni, Pierre-François Dequin, Gérard Oliviero, Alain Gourlaouen, Caroline Piau, Marie-France Lutz-Murphy, Benoît Claude, Jean-Paul Aubry, Nadine Dubosc-Marchenay, Kamilla Chraibi, Emmanuelle Heusse, Sylvain Le Chevallier, Nathalie Brieu, Farid Sifaoui, Lorraine Letranchant, Hélène Durox, Jean-Pierre Lagasse, Adel Ghedira, Xavier Roubert, Fatma Magdoud, Hélène Jean-Pierre, Etienne Carbonelle, Olivier Dereeper, Lionel Carbillon, Christophe Billy, Mélanie Roblin, Marie-José Kodzin, Philippe Niel, Solène Makdessi, Matteo Vassallo, Maryse Archambaud, Fabian Haccourt, Didier Blaise, Stéphane Bourgeois, Elena Marcu, Charles Kubiak, Brisse Castel, François Guinet, Marie Pouzoullic, Frédérique Nathan-Bonnet, Vincent Gendrin, Céline Becherrawy, Aline Secher, Pierre Abgueguen, Clarence Eloy, Jean-Marc Tourani, Frédéric Klapczynski, Bernard Montmasson, Philippe Real, Joanna Pofelski, Yves Welker, Karim Krechiem, Eric Caumes, Martine Elena-Daumas, Christophe Saigne, Gilles Hittinger, Chantal Delesalle, Jonathan Messika, Fabrice Lesage, Daniela Pop, Daniel Coetmeur, Renato Colamarino, Chetaou Mahaza, Patrick Plésiat, Isabelle Fredenucci, Mylène Baret, Guy Mager, Pascale Chavel, Isabelle Labourdette, Anne-Claude Menguy, Nicolas Fortineau, Ludovic Le Sec, Valérie Gauduchon, Francis Barraud, Nicolas Letellier, Didier Vincent, Janine Frey, Philippe Riegel, Michel Pavic, Jean-Luc Fabre, Jean-Pierre Fauchart, Alain Goudeau, Stéphanie Husson-Wetzel, Philippe Eymerit, Mohamed Camara, Nathalie Seta, Elisabeth Carole Ngo Bell, Philippe Repellin, Laurent Alric, Vincent Leroy, Françoise Delisle Mizon, Jean-Philippe Emond, Marie-Françoise Borie, Lise Crémet, Wladimir Chelle, Elisabeth Brottier-Mancini, Bernard Garrigues, Claire Letellier, Loïc Geffray, Frédéric Méchaï, Julien Bador, Benoit Guery, Alain-Charles Fouilhoux, Corinne Dagada, Pierre Duhaut, Julien Goustille, Arnaud Sément, Francis Carcenac, Isabelle Girard-Buttaz, Claire Chapuzet, Fabienne Jouatte, Bruno Riou, Fabrice Hayoun, Chloé Di Meglio, Youssef Ali, Michel Leneveu, Nathalie Montagne, Yves Garcera, Audrey Moustache, Pierre-Eric Danin, Geneviève Le Lay, Dominique Courouge-Dorcier, Isabelle Worcel, Emmanuel Morelon, Vincent Pestre, Jean-Pierre Vilque, Jean-Christophe Paquet, Lucien Bodson, Anne-Marie Forest, Fabrice Pierre, Christian Pommier, Fabien Dutasta, Pierre Fournel, Stéphanie Courtois, Elodie Dubois, Serge Vanden Einjden, Patrick Honderlick, Pascal Richette, Fabienne Tamion, Véronique Chassy, Richard Megbemado, Anne-Marie Le Reste, Bernard Simian, Henri Osman, Anthony Texier, Badih Ayach, François Simon, Jean-Michel Filloux, Béatrice Dubourdieu, Jean-Claude Semet, Sarah Kubab, Tawfiq Henni, Patrick Dudeffant, Delphine Hequet, Olivier Mimoz, Marc Auburtin, Amélie Chabrol, Mickael Bonnan, Caroline Léonnet, Claire Wintenberger, Serge Ilunga, Patrice Lanba, Sophie Rosello, Alexandre Damage, Flore Bouche, Michel Thibault, Frederic Faibis, Chantal Dhennain, Jean-Philippe Talarmin, Armelle Lamour, Remi Boussier, Fabien Garnier, Marie-Laure Brival, Nourredine Hedjem, Philippe Vande-perre, Raphaël Coint, Jean-Claude Reveil, Eva Weinbronn, Emmanuelle Lavalard, Alexandra Fille, Françoise Le Turdu, Lionel Leroux, Jean-Yves Lefrant, Jean Berthet, Radia Bouaziz, Alain Ravaud, Sylvaine Rousseau, Yacine Merrouche, Alain Le Coustumier, Bertrand Guider, Gisèle Dewulf, Jean-Marc Faucheux, Jacques Piquet, Franck Leibinger, Charles Cerf, Robin Stephan, Jean-Philippe Redonnet, Jean-Paul Stahl, Ella Dzeing, Simona Pavel, Guy Vernet, Ghada Hatem, Samer Kayal, Jacques Deschamps, Dominique Descamps, Marion Levast, Marc Bouiller, Sylvie Dargere, Claire Dingremont, Stéphane Gaudry, François Maillot, Sylvie Odent, Nathalie Cervantes, Hélène Zanaldi, Laurence Gachassin, Olivier Ruyer, David Patin, Benoît Cazenave, Pascal Jacquier, Michelle Boyer, Béatrice Berteaux, Virginie Zarrouk, Jacques Bor, Isabelle Legoff, Hélène Albinet, Florence Rousseau, Gilles Pialoux, Guenaelle Salaun-Beretta, Alexandra Moura, Véronique Vernet Garnier, Didier Lepelletier, Pierre-Alexandre Hauss, Joëlle Belaisch-Amart, Didier Lepeletier, Jacob Xavier, Aline Nare, Annie Motard-Picheloup, Alain Améri, Bertrand Lioger, Jean-Valère Malfuson, Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Département de Médecine interne [Lariboisière], Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Biologie des Infections - Biology of Infection, Service de Gynécologie et Obstétrique [Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Investigation Clinique et d’Accès aux Ressources Biologiques (Plate-forme) - Clinical Investigation and Access to BioResources (ICAReB), Institut Pasteur [Paris] (IP), Infectious Disease Department [Saint Maurice], Agence Nationale de la Santé Publique [Saint-Maurice] (ANSP), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), CHU Amiens-Picardie, Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, MONALISA study group, Programme Hospitalier Recherche Clinique, Institut Pasteur, Inserm, French Public Health Agency., ROZIER, marie-Claire, CHU Necker - Enfants Malades [AP-HP], Centre National de Référence Listeria - Biologie des Infections (CNRL), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria - Biologie des Infections (CCOMS), CHU Pitié-Salpêtrière [APHP], Hôpital Lariboisière-Université Paris Diderot - Paris 7 (UPD7), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP], Institut Pasteur [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Centre National de Référence Listeria - Biologie des Infections ( CNRL ), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre collaborateur de l'OMS (CCOMS) des Listeria ( CCOMS ), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité ( CRESS (U1153 / UMR_A 1125) ), Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie ( UPMC ), Université Pierre et Marie Curie - Paris 6 ( UPMC ), Université Paris Diderot - Paris 7 ( UPD7 ) -Hôpital Lariboisière, Biologie des Infections, Institut Pasteur [Paris]-Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Investigation Clinique et d’Accès aux Ressources Biologiques (Plate-forme) - Clinical Investigation and Access to BioResources ( ICAReB ), Agence Nationale de la Santé Publique [Saint-Maurice] ( ANSP ), Assistance Publique - Hôpitaux de Paris, Assistance publique - Hôpitaux de Paris (AP-HP), Université Paris Descartes - Paris 5 ( UPD5 ), Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)
Subjects: Bacteremia/epidemiology/mortality, 0301 basic medicine, Male, Pediatrics, bacteraemia, Infectious Disease Transmission, [SDV]Life Sciences [q-bio], Bacteremia, France/epidemiology, Infant, Newborn, Diseases, Foodborne Diseases, Meningoencephalitis, Pregnancy, Risk Factors, Vertical, Medicine, Listeriosis, Prospective Studies, Pregnancy Complications, Infectious, Prospective cohort study, ddc:618, diabetes, alcoholism, Hazard ratio, Foodborne Diseases/microbiology, immuno suppressive therapies, Prognosis, 3. Good health, [SDV] Life Sciences [q-bio], Hospitalization, Infectious Diseases, isolates, Population Surveillance, Female, France, Listeria monocytogenes/classification/isolation & purification, Cohort study, Adult, medicine.medical_specialty, 030106 microbiology, Notifiable disease, Listeriosis/diagnosis/epidemiology/microbiology, Context (language use), macromolecular substances, 03 medical and health sciences, Humans, study, Aged, [ SDV ] Life Sciences [q-bio], business.industry, Public health, cirrhosis, Infant, Newborn, Infant, Diseases/epidemiology/microbiology, HIV, Mandatory Reporting, Newborn, medicine.disease, Listeria monocytogenes, infection, Infectious Disease Transmission, Vertical, Pregnancy Complications, Infectious/epidemiology/microbiology, Meningoencephalitis/epidemiology/microbiology/mortality, Observational study, business, prognostic, mellitus
Abstract: International audience; Evidence before this study We searched PubMed on June 30, 2016, for English-language cohort studies published since Jan 1, 1980, of patients with invasive listeriosis worldwide with the keywords " listeria " , " listeriosis " , " maternal " , and " neurolisteriosis ". Studies had to include epidemiological or clinical data on listeriosis. All clinical forms of infection were included (bacteraemia, neurolisteriosis, and maternal–neonatal infection). Host risk factors for listeriosis have been well identified, but the clinical features and prognostic factors of the disease are based on retrospective studies compiling heterogeneous data or random collected cases. Furthermore, no clinical trial has ever been done and medical management is not evidence based. Added value of the study Our study is the first prospective clinical study focusing on all forms of invasive listeriosis. The study is based on a national mandatory system that allowed the nearly complete capture of microbiologically proven cases. The study shows a higher burden of listeriosis than reported before: more than 80% of infected mothers experienced major fetal or neonatal complications (fetal loss, very high prematurity, early or late onset disease); only 39% of patients with neurolisteriosis survived and fully recovered. The study provides important new data to improve management and predict outcome in listeriosis, such as determination of the time window for fetal losses (
Published: 2016
Full Text: View/download PDF

99. Could we predict airborne Aspergillus contamination during construction work?

Author: I. Alaabouche, J.-R. Zahar, Benoit Pilmis, Marie-Elisabeth Bougnoux, Emmanuel Weiss, Valérie Thépot-Seegers, and C. Angebault
Subjects: 0301 basic medicine, Veterinary medicine, Epidemiology, 030106 microbiology, Air Microbiology, 030501 epidemiology, Aspergillosis, Aspergillus fumigatus, 03 medical and health sciences, medicine, Infection control, Humans, Hospital Design and Construction, Prospective Studies, Hospitals, Teaching, Aspergillus, Infection Control, biology, business.industry, Health Policy, Public Health, Environmental and Occupational Health, Outbreak, Contamination, biology.organism_classification, medicine.disease, Infectious Diseases, Work (electrical), Demolition, France, 0305 other medical science, business, Environmental Monitoring
Abstract: Background Aspergillus fumigatus is a major opportunistic pathogen causing nosocomial infection. Hospital outbreaks of invasive aspergillosis have been associated with demolition and building construction. This study was designed to examine the impact of meteorologic factors and different periods of work on outdoor fungal airborne concentrations. Methods The study was conducted at Necker Enfants Malades Hospital, a 650-bed teaching care hospital recently involved in a large construction program, including renovation, construction, and demolition. During the work phases, prospective external air samplings were performed 3 times a week, and meteorologic parameters were collected every day. Results Two hundred and one samples were collected. Aspergillus spp were found in 80.1% of samples, with a median concentration of 16 colony forming units (CFU)/m 3 . A significant increase in the colony count of molds occurred after demolition. In the multivariate analysis, factors associated with overall fungi concentration were the type of work construction and temperature. Elevated Aspergillus spp concentrations (>20 CFU/m 3 ) were associated with higher temperature. Conclusions Our findings underline the importance of environmental surveillance. According to our results we suggest that demolition work should be performed during the winter and fall seasons.
Published: 2016

100. Extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae in community-acquired urinary tract infection (UTI)

Author: Benoit Pilmis
Published: 2016
Full Text: View/download PDF

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Publication Type

Journal

Database

Publisher

130 results on '"Benoit Pilmis"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources