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In order to carry out aircraft structural load safety monitoring and accumulate relevant structural load data for aircraft fatigue life assessment, it is necessary to establish aircraft structural load model related to flight parameters. For the nonlinear relationship between aircraft structural loads and flight parameters, the sequential minimal optimization (SMO) algorithm with improved stopping criterion and the particle swarm optimization algorithm are used to improve the support vector machine regression method, and the flight parameters involved in the modeling are selected by the method of flight dynamics analysis combined with the Pearson correlation coefficient. Taking the transonic pitching maneuver of an aircraft as an example, a structural shear model of a wing is established, and the modeling method is verified by simulation. The results show that the accuracy of improved support vector machine regression method is better than the original method. It is concluded that the improved support vector machine regression method can improve the accuracy and generalization ability of the established model.

This experiment was conducted to investigate the effects of live yeast and yeast cell wall polysaccharides on growth performance, rumen function and plasma lipopolysaccharides (LPS) content and immunity parameters of beef cattle. Forty Qinchuan cattle were randomly assigned to one of four treatments with 10 replicates in each treatment. The dietary treatments were: control diet (CTR), CTR supplemented with 1 g live yeast (2×1010 live cell g−1 per cattle per day (YST1), CTR supplemented with 2 g live yeast per cattle per day (YST2) and CTR supplemented with 20 g of yeast cell wall polysaccharides (30.0%≤β-glucan≤35.0%, and 28.0%≤mannanoligosaccharide≤32.0%) per cattle per day (YCW). The average daily gain was higher (P=0.023) and feed conversion ratio was lower (P=0.042) for the YST2 than the CTR. The digestibility of neutral detergent fiber (P=0.039) and acid detergent fiber (P=0.016) were higher in yeast supplemented groups. The acetic acid:propionic acid of the YST2 was lower compared with the CTR (P=0.033). Plasma LPS (P=0.032), acute phase protein haptoglobin (P=0.033), plasma amyloid A (P=0.015) and histamine (P=0.038) were lower in the YST2 compared with the CTR. The copies of fibrolytic microbial populations such as Fibrobacter succinogenes S85, Ruminococcus albus 7 and Ruminococcus flavefaciens FD-1 of the YST2 were higher (P

Objective To investigate the teaching needs of clinical skills and scientific research skills in standardized training of surgical residents and to crient teaching remodeling. Methods A self-filling questionnaire survey was conducted in July 2018. Surgical residents of Peking Union Medical College Hospital, who are at the second and the third year of training, were gathered to sit in separate in classrooms. The filling methods and matters needing attention were explained uniformly. The trainees filled in the form of “back-to-back” by themselves. Totally 85 questionnaires were collected and 83 questionnaires were valid. This paper mainly investigates the training needs of clinical diagnosis and treatment, operation skills and scientific research skills. Results The demand of clinical skills is mostly manifested in the diagnosis and treatment of common surgical diseases and surgical teaching. The most demanded surgical teaching was “hand-holding” and acting as a assistant or master knife. The main requirements of scientific research skills are data collection, statistical methods (Meta analysis), scientific research design and project establishment, tender writing and bidding, and paper writing and publication. Conclusions The needs of surgical residents reflect their deficiencies in the diagnosis and treatment of common diseases, operation skill and research capacity. For this reason, the Surgical Teaching Group has put forward such reform schemes as “tutorial system” “animal model” “all-factor simulated surgery” “more scientific research training courses” and so on.

MnO2@Ni(OH)2 core/shell structure nanowire arrays (NWAs) supported on carbon cloth were successfully synthesized by a two-step method, applied in flexible all-solid-state asymmetric supercapacitors (ASCs). The Ni(OH)2 nanosheets wrapped on the surface of each MnO2 nanowire uniformly, which can increase the capacitance of MnO2 NWAs to a high specific capacitance of 432.8F/g at 5mV/s. The electrode also exhibits good cycling ability, 92.3% of the initial capacity can be remained after 2000 cycles at 5A/g. The assembled MnO2@Ni(OH)2//MnO2 asymmetric device with a maximum voltage of 1.8V has been fabricated, delivering both high energy density (69.2Wh/kg) and power density (4.5kW/kg at 54.6Wh/kg). These results show that MnO2@Ni(OH)2 NWAs with large specific surface area, combined with the flexible carbon cloth substrate can be applied in supercapacitor field in large scale.

Liver injury threatens the overall health of an organism, as it is the core organ of the animal body. Liver metabolism is affected by numerous factors, with dietary energy level being a crucial one. Therefore, the present study aimed to evaluate hepatic injury and to describe its metabolic mechanism in ruminants fed diets with different dietary energy levels. A total of 25 Yunnan semi-fine wool sheep were fed diets with five dietary metabolic energy levels and were randomly assigned to five groups as follows: low energy (LE), medium–low energy (MLE), medium energy (ME), medium–high energy (MHE), and high energy (HE). The results revealed that the average optical density (AOD) of lipid droplets in the LE, MLE, and HE groups was higher than that in the ME and MHE groups. The enzyme activity of alanine aminotransferase (ALT) was the lowest in the ME group. An increase in dietary energy level promoted the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity and altered the malondialdehyde (MDA) and protein carbonyl (PCO) concentration quadratically. In addition, both high and low dietary energy levels upregulated the mRNA abundance of proinflammatory cytokine interleukin (IL)-1β, nuclear factor-kappa B (NF-κB), and tumor necrosis factor (TNF)-α. Metabonomic analysis revealed that 142, 77, 65, and 108 differential metabolites were detected in the LE, MLE, MHE, and HE groups, compared with ME group respectively. These metabolites were involved in various biochemical pathways, such as glycolipid, bile acid, and lipid metabolism. In conclusion, both high and low dietary energy levels caused hepatic injury. Section staining and metabonomic results revealed that hepatic injury might be caused by altered metabolism and lipid accumulation induced by lipid mobilization.

This study aimed to evaluate the effect of enzymatic hydrolysate of cottonseed protein (ECP) on the kinetic of gas production, rumen fermentation characteristics, and microbial diversity in continuous in vitro culture with a single factorial design of supplementation with various concentrations of ECP or yeast culture. Treatments were control (without supplementation, CON), supplementation with 10 g/kg Diamond-V XP yeast culture of substrate (XP), and supplementation with 6, 12 and 18 g/kg ECP of substrate (ECP1, ECP2, ECP3), each incubated with 30 mL of buffered incubation fluids and 200 mg of fermentation substrate in graduated glass syringes fitted with plungers for 48 h. Compared with the CON treatment, supplementation of XP yeast culture increased the cumulative gas production at 12 and 24 h, the concentration of ammonia nitrogen (NH3-N) concentration at 24 and 36 h, the concentration of microbial protein (MCP) concentration at 24 and 48 h, the molar butyrate proportion at 12, 24, and 48 h, the molar valerate proportion at 48 h, and the ratio of non-glucogenic to glucogenic acids (p < 0.05). Compared with the CON treatment, the concentration of MCP and the molar propionate proportion at 12 h were higher in the ECP1 treatment (p < 0.05); the cumulative gas production at 2, 4, and 12 h, the concentration of NH3-N at 36 h and the molar valerate proportion at 48 h were higher in the ECP2 treatment (p < 0.05); the cumulative gas production at 2, 12, and 48 h, the concentration of NH3-N at 12 and 36 h, the concentration of MCP at 12, 36, and 48 h, the molar butyrate proportion at 12 and 48 h, and the molar valerate proportion at 48 h were higher in the ECP3 treatment (p < 0.05). Compared with the CON treatment, supplementation with XP yeast culture significantly altered the relative abundance of the phyla Firmicutes, Kiritimatiellaeota, and Proteobacteria, while supplementation with ECP had minimal effect on bacterial diversity. The prediction of bacterial functions showed that the main gene functions of rumen bacteria are associated with carbohydrate metabolism, amino acid metabolism, and membrane transport. The findings of this study suggest that ECP can be used as a superior feed ingredient for ruminants, the suitable level of ECP was 18 g/kg in vitro experiment.

Objective This experiment was conducted to compare the structure and composition of ruminal microorganisms in goats with high and low neutral detergent fibre (NDF) digestibility. Methods Nineteen crossbred goats were used as experimental animals and fed the same total mixed rations during the 30-day pre-treatment and 6-day digestion trialperiods. All faeces were collected during the digestion period for measuring the NDF digestibility. Then, high and the low NDF digestibility individuals were chosen for the high NDF digestibility group (HFD) and low NDF digestibility group (LFD), respectively. Rumen contents were collected for total microbial DNA extraction. The V4 region of the bacterial 16S rRNA gene was amplified using universal primers of bacteria and sequenced using high-throughput sequencer. The sequences were mainly analysed by QIIME 1.8.0. Results A total of 18,694 operational taxonomic units were obtained, within 81.98% belonged to bacteria, 6.64% belonged to archaea and 11.38% was unassigned microorganisms. Bacteroidetes, Firmicutes, and Proteobacteria were the predominant microbial phyla in both groups. At the genus level, the relative abundance of fifteen microorganisms were significantly higher (p

Objective To explore the efficacy and safety of Vemurafenib in acral and mucosal melanoma patients with BRAF gene mutation. Methods We retrospectively analyzed the clinical data of 24 patients diagnosed as BRAF gene-mutant acral melanoma (AM) or mucosal melanoma (MM) hospitalized in Peking University Cancer Hospital from January 2011 to January 2016. All patients had died by the follow-up cutoff date of January 2017. Fisher exact test was used to detect the difference of baseline characteristics between AM and PM patients. Kaplan-Meier method was used for the estimation of overall survival (OS) and progression-free survival (PFS). We compared patients' survival data between two melanoma types using Log rank test. Results There was no statistical difference in baseline characteristics between AM and MM patients. Overall median OS and PFS were 8.0 and 4.9 months. No statistical difference was found in median OS, median PFS or disease control rate. The severity of most adverse events was less than grade 3. Vemurafenib was well tolerated during patients' treatment. Conclusion Vemurafenib has acceptable efficacy and safety in BRAF-gene-mutant AM and MM patients, which is similar to that in cutaneous melanoma patients.

Objective To establish a rapid and high efficient method of primary culture of BCAFs by improving primary culture methods of common breast cancer-associated fibroblasts (BCAFs). Methods We took cancerous tissue samples from the patients diagnosed as invasive breast cancer. All samples were randomly divided into two groups: the modified group and the conventional group with same size and quality. The modified group was digested with a modified enzyme digestion method (typeⅡcollagenase+hyaluronidase digestion for 10 min). The conventional group was digested with conventional enzymatic digestion method (typeⅠcollagenase digestion for 8h). The total number of cells obtained, the activity of adherent cells after 48h culture and the purity of BCAFs between two groups were compared by cell counting, MTT and immunofluorescence. The characteristics of primary BCAFs were observed under inverted microscope. Results The amount of cells, the activity of adherent cells after 48h culture and cells purity obtained by the modified enzyme digestion method were all higher than those by the conventional enzyme digestion method (P < 0.001). The primary BCAFs obtained were spindle-shaped or spindle-shaped, with many cytoplasmic particles and irregular cell arrangement. Conclusion A rapid and high efficient primary culture method of BCAFs is established successfully, as the foundation for the later study of its function in breast cancer.

PurposeThe prognostic value of caveolin-1 in prostate cancer remains uncertain. Hence, this meta-analysis was performed to evaluate the prognostic value of caveolin-1 in prostate cancer, as well as ascertain the relationship between caveolin-1 expression and clinicopathological characteristics of prostate cancer patients.MethodsThe PubMed, Embase, Chinese National Knowledge Infrastructure and Chinese Biology Medicine databases were electronically searched to retrieve published studies on caveolin-1 expression in prostate cancer. After study selection and data extraction, the meta-analysis was conducted using Review manager 5.3 software. Odds ratio (OR) with 95% confidence interval (CI) was used to estimate the pooled effect. Funnel plot was used to assess publication bias.ResultsA total of ten studies were enrolled, which included 3976 cases of prostate cancer, 72 cases of high-grade intraepithelial neoplasia (HGPIN), and 157 normal controls. Results of the meta-analysis showed that the positive rate of caveolin-1 expression in prostate cancer was 18.28 times higher than that in normal control (OR= 18.28, 95% CI: 9.02–37.04, p10 vs. ≤ 10 (OR=2.09, 95% CI: 1.35–3.22, p

In ruminants, the bacterial community in the gastrointestinal tract (GIT) has an essential role in healthy growth. Examining the bacterial composition in the GIT between growth-retarded and normal yaks could improve our understanding of the role of microorganisms in yaks with growth retardation. In this study, eight male yaks with growth retardation were used as the growth-retarded yak (GRY) group, and another eight male growth normal yaks (GNYs) with the same breed and age were used as the GNY group. We compared the bacterial community in the rumen, duodenum, jejunum, ileum, cecum, and colon between GRY and GNY groups based on the 16S ribosomal RNA gene sequencing. Alpha-diversity revealed that the Shannon index in the duodenum and ileum of the GNY group was higher (P < 0.05) than that of the GRY group. However, the opposite trend was found in the jejunum and cecum. The principal coordinates analysis (PCoA) showed that the bacterial structure in all segments of GIT differed from each other between two groups. In the rumen, the relative abundances of Ruminococcaceae NK4A214 group, Ruminococcaceae UCG-014, and Treponema 2 were higher (P < 0.05) in the GNY group as compared with the GRY group. However, the Christensenellaceae R-7 group exhibited an opposite trend. In the jejunum, compared with the GNY group, the unclassified Chitinophagaceae was enriched significantly (P < 0.05) in the GRY group. However, the unclassified Peptostreptococcaceae, Christensenellaceae R-7 group, and Lachnospiraceae NK3A20 group were enriched (P < 0.05) in the GNY group. In the ileum, the relative abundances of the Rikenellaceae RC9 gut group and Prevotellaceae UCG-004 were higher (P < 0.05) in the GNY group than those in the GRY group. In the cecum, the GNY group showed a higher (P < 0.05) relative abundance of Prevotellaceae UCG-003 as compared with the GRY group. In the colon, the relative abundances of Treponema 2 and unclassified Lachnospiraceae were slightly higher (0.05 < P < 0.10) in the GNY group than those in the GRY group. Overall, these results improve our knowledge about the bacterial composition in the GIT of growth-retarded and normal yaks, and regulating the bacterial community may be an effective solution to promote the compensatory growth of GRYs.

Background Yak (Bos grunniens) is an ancient bovine species on the Qinghai-Tibetan Plateau. Due to extremely harsh condition in the plateau, the growth retardation of yaks commonly exist, which can reduce the incomes of herdsman. The gastrointestinal barrier function plays a vital role in the absorption of nutrients and healthy growth. Functional deficiencies of the gastrointestinal barrier may be one of the contributors for yaks with growth retardation. Methods To this end, we compared the growth performance and gastrointestinal barrier function of growth-retarded (GRY) and normal yaks (GNY) based on average daily gain (ADG), serum parameters, tissue slice, real-time PCR, and western blotting, with eight yaks in each group. Results GRY exhibited lower (P

The present research was conducted to evaluate the connection between the true digestibility of Phosphorus (TDP) in diet and bacterial community structure in the gastrointestinal tract (GIT) of goats. Twenty-eight Nubian goats were chosen and metabolic experiment was conducted to analyze TDP of research animals. Eight goats were grouped into the high digestibility of phosphorus (HP) phenotype, and another 8 were grouped into the low digestibility of phosphorus (LP) phenotype. And from the rumen, abomasum, jejunim, cecum and colon content of the goats, bacterial 16S rRNA gene amplicons were sequenced. In the rumen 239 genera belonging to 23 phyla, in abomasum 319 genera belonging to 30 phyla, in jejunum 248 genera belonging to 36 phyla, in colon 248 genera belonging to 25 phyla and in cecum 246 genera belonging to 23 phyla were noticed. In addition, there was a significant correlation between the TDP and the abundance of Ruminococcaceae_UCG-010, Ruminococcus_2, Ruminococcaceae_UCG-014, Selenomonas_1 and Prevotella in the rumen, Lachnospiraceae_ND3007_group, Saccharofermentans, Ruminococcus_1, Ruminococcaceae_UCG-014, Lachnospiraceae_XPB1014_group and Desulfovibrio in the abomasum, Prevotella, Clostridium_sensu_stricto_1, Fibrobacter, Desulfovibrio and Ruminococcus_2 in the jejunum, Ruminococcaceae_UCG-014 in the colon, and Desulfovibrio in the cecum. Present research trial recommended that the community of gastrointestinal microbiota is a factor affecting TDP in goats.

This work was aimed to investigate the effects of the different particle size of ground alfalfa hay on caecal microbial and archeal communities of rabbits. One hundred-twenty New Zealand rabbits (950.3 ± 8.82 g) were allocated into four treatments, with five replicates in each treatment and six rabbits in each replicate. The particle sizes of the alfalfa meal in the four treatment diets were 2,500, 1,000, 100 and 10 µm respectively, while the other ingredients were ground through a 2.5 mm sieve. High-throughput sequencing technology was applied to examine the differences in bacteria and methanogenic archaea diversity in the caecum of the four treatment groups of rabbits. A total of 745,946 bacterial sequences (a mean of 31,081 ± 13,901 sequences per sample) and 539,227 archaeal sequences (a mean of 22,468 ± 2,443 sequences per sample) were recovered from twenty-four caecal samples, and were clustered into 9,953 and 2,246 OTUs respectively. A total of 26 bacterial phyla with 465 genera and three archaeal phyla with 10 genera were identified after taxonomic summarization. Bioinformatic analyses illustrated that Firmicutes (58.69% ∼ 68.50%) and Bacteroidetes (23.96% ∼ 36.05%) were the two most predominant bacterial phyla and Euryarchaeota (over 99.9%) was the most predominant archaeal phyla in the caecum of all rabbits. At genus level, as the particle size of alfalfa decreased from 2,500 to 10 µm, the relative abundances of Ruminococcaceae UCG-014 (P

Objective To assess the efficacy and safety of paclitaxel, ifosfamide and cisplatin (TIP) as the first-line treatment for the patients with intermediate- or poor-risk testicular cancer retrospectively. Methods Patients with intermediate- or poor-risk testicular cancer in our center received TIP as the first-line treatment. Objective response rate, 2-year progression free survival(PFS) and overall survival(OS) were evaluated. Results The mean treatment of 20 patients was 4.25cycles. Six patients achieved CR(30%), seven patients had partial response(35%) and overall response rate was 65%. The median follow-up time was 25 months. Three patients died of tumor progression. Hematologic toxicity was most common(95%), with 30% of grade 3-4 neutropenia. Estimated 2-year PFS was 72.9% and OS was 89.1%. Conclusion Paclitaxel, ifosfamide and cisplatin(TIP) are safe and effective as the first-line treatment on patients with intermediate- or poor-risk testicular cancer. Compared with previous studies, 2-year PFS and OS have been improved. Adverse effect could be controlled. Further researches are still needed.

BackgroundThe accuracy of Convolution/Superposition (CS) algorithm is considered to be next to Monte Carlo algorithm (MC) for radiotherapy dose calculation algorithm. Although the calculating speed of this algorithm is much faster than that of MC, its calculating speed can not fully meet the clinical requirements. With the aid of a single graphics processing unit GPU (Tesla C1060), the CS algorithm can be accelerated to 60 times faster than the traditional CPU serial calculation. The calculating time for single field is about 1 min which can be used in some simple three dimensional conformal radiotherapy planning (3DCRT), but this calculating speed does not satisfy the speed need for intensity modulated radiation therapy (IMRT) planning.PurposeThis study aims to explore a faster calculating speed solution of CS algorithm applied to IMRT with multi GPU.MethodsThe acceleration scheme of CPU + multi GPU heterogeneous model was analyzed by using different number of GPUs. High-end GPU, i.e., Tesla C2015, was used for experimental test of CS algorithm executing under the compute unified device architecture (CUDA) platform. Speeds of different number GPUs combined with CPU were compared to find the suitable solution.ResultsThe experimental results show that the speedup of CS algorithm is not completely linear with the number of GPUs. With reasonable number of GPUs and optimized program codes, the computing time of CS algorithm for single field radiotherapy dose calculation can be reduced to 9 seconds by using 7 high-end GPUs (Tesla C2015), 207 times faster than that of a single CPU.ConclusionsWith the implementation of multi GPU heterogeneous model and code optimization, the CS algorithm can be applied to clinical IMRT treatment planning.

This study aimed to investigate the effects of N-carbamylglutamate (NCG) supplementation on the follicular development of yaks to identify potential mechanisms essential for fertility in yaks. Twelve multiparous anoestrous female yaks were randomly assigned to two groups—Control (fed with a basal diet, n = 6) and NCG (basal diet supplemented with 6.0 g day−1 NCG, n = 6). Yaks in the NCG group had higher numbers of large follicles (>5 mm in diameter) than those in the Control group. An RNA-sequencing analysis of yak ovaries revealed a total of 765 genes were differentially expressed between experimental groups, of which 181 genes were upregulated and 584 genes were downregulated following NCG supplementation. The results of a transcriptome functional analysis, qRT-PCR validation, and immunohistochemistry revealed that NCG supplementation increased angiogenesis and de novo synthesis of cholesterol in yak ovaries. NCG was also found to upregulate the gene expression of steroidogenic enzymes. Based on this, it was concluded that NCG supplementation promotes the follicular development of yaks mainly by affecting cholesterol metabolism to initiate steroidogenesis in ovaries. The results provide evidence for understanding the mechanisms responsible for NCG promoting follicular development of female yaks, which may contribute to the development and application of NCG in animal reproduction.

This study was conducted to investigate the phylogenetic diversity of archaea in the rumen of adult and elderly yaks. Six domesticated female yaks, 3 adult yaks ((5.3±0.6) years old), and 3 elderly yaks ((10.7±0.6) years old), were used for the rumen contents collection. Illumina MiSeq high-throughput sequencing technology was applied to examine the archaeal composition of rumen contents. A total of 92 901 high-quality archaeal sequences were analyzed, and these were assigned to 2 033 operational taxonomic units (OTUs). Among these, 974 OTUs were unique to adult yaks while 846 OTUs were unique to elderly yaks; 213 OTUs were shared by both groups. At the phylum level, more than 99% of the obtained OTUs belonged to the Euryarchaeota phylum. At the genus level, the archaea could be divided into 7 archaeal genera. The 7 genera (i.e., Methanobrevibacter, Methanobacterium, Methanosphaera, Thermogymnomonas, Methanomicrobiu, Methanimicrococcus and the unclassified genus) were shared by all yaks, and their total abundance accounted for 99% of the rumen archaea. The most abundant archaea in elderly and adult yaks were Methanobrevibacter and Thermogymnomonas, respectively. The abundance of Methanobacteria (class), Methanobacteriales (order), Methanobacteriaceae (family), and Methanobrevibacter (genus) in elderly yaks was significantly higher than in adult yaks. In contrast, the abundance of Thermogymnomonas in elderly yaks was 34% lower than in adult yaks, though the difference was not statistically significant. The difference in abundance of other archaea was not significant between the two groups. These results suggested that the structure of archaea in the rumen of yaks changed with age. This is the first study to compare the phylogenetic differences of rumen archaeal structure and composition using the yak model.

Pig and poultry production in China had experienced considerable changes from 1960 to 2010. The present study aimed to evaluate the effects of these changes on greenhouse gas emission inventories (expressed as CO2 equivalent) from these two sectors. The inventories included methane emissions from enteric fermentation, methane and nitrous oxide production from manure management. The greenhouse gas emissions from these sources in 2010 in pig sector were 17, 62 and 21%, respectively, and that in poultry sector (including chicken, duck, goose and others) were 1, 18 and 81%, respectively. Total CO2 equivalent increased from 1960 to 2010 in both pig (11 582 to 55 564 Gg yr−1) and poultry (1 497 to 14 873 Gg yr−1) sectors. Within poultry sector, emissions from chicken, duck, goose and others accounted for 74, 15, 11 and 0.01% in 2010, respectively. However, during the last 50 years, these emissions continuously reduced when related to production of 1 kg of pork (8.01 to 1.14 kg kg−1), poultry meat (1.19 to 0.37 kg kg−1) and egg (0.47 to 0.33 kg kg−1), which is mainly associated with the continuous improvement in production efficiency in all management systems. These results provide benchmark information for Chinese authorities to develop appropriate policies and mitigation strategies to reduce greenhouse gas emissions from pig and poultry sectors.

Abstract The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals. Little information has been reported on these aspects of the goat GIT. This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon. Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers. The amplicons were sequenced on an Illumina MiSeq platform, and the biological information was analyzed using QIIME software. A total of 857 genera that belonged to 39 phyla were observed across the goat GIT, with Bacteroidetes and Firmicutes dominating. Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT. However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences. The study indicated that there were significant differences in microbial function among the GIT regions. In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine. Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions. In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.

Growth retardation reduces the incomes of livestock farming. However, effective nutritional interventions to promote compensatory growth and the mechanisms involving digestive tract microbiomes and transcripts have yet to be elucidated. In this study, Qinghai plateau yaks, which frequently suffer from growth retardation due to malnutrition, were used as an experimental model. Young growth-retarded yaks were pastured (GRP), fed basal ration (GRB), fed basal ration addition cysteamine hydrochloride (CSH; GRBC) or active dry yeast (ADY; GRBY). Another group of growth normal yak was pastured as a positive control (GNP). After 60-day nutritional interventions, the results showed that the average daily gain (ADG) of GRB was similar to the level of GNP, and the growth rates of GRBC and GRBY were significantly higher than the level of GNP (P < 0.05). Basal rations addition of CSH or ADY either improved the serum biochemical indexes, decreased serum LPS concentration, facilitated ruminal epithelium development and volatile fatty acids (VFA) fermentation of growth-retarded yaks. Comparative transcriptome in rumen epithelium between growth-retarded and normal yaks identified the differentially expressed genes mainly enriched in immune system, digestive system, extracellular matrix and cell adhesion pathways. CSH addition and ADY addition in basal rations upregulated ruminal VFA absorption (SLC26A3, PAT1, MCT1) and cell junction (CLDN1, CDH1, OCLN) gene expression, and downregulated complement system (C2, C7) gene expression in the growth-retarded yaks. 16S rDNA results showed that CSH addition and ADY addition in basal rations increased the rumen beneficial bacterial populations (Prevotella_1, Butyrivibrio_2, Fibrobacter) of growth-retarded yaks. The correlation analysis identified that ruminal VFAs and beneficial bacteria abundance were significantly positively correlated with cell junction and VFA absorption gene expressions and negatively correlated with complement system gene expressions on the ruminal epithelium. Therefore, CSH addition and ADY addition in basal rations promoted rumen health and body growth of growth-retarded yaks, of which basal ration addition of ADY had the optimal growth-promoting effects. These results suggested that improving nutrition and probiotics addition is a more effective method to improve growth retardation caused by gastrointestinal function deficiencies.

Reproductive efficiency is the main factor limiting yak production on the Tibet Plateau. The purpose of this study was to investigate the effect of supplementation with calcium chloride (CaCl) and monocalcium phosphate (MCP) for 30 days before breeding on body weight (BW) change, serum bone metabolism biomarkers, conception rate, and calving rate of grazing yaks. Ninety 3 year old yak heifers (153.05 ± 6.56 kg BW) were assigned to three treatments (n = 30 per treatment): grazing without supplementation (CONT), grazing plus calcium chloride supplementation (CaCl), and grazing plus monocalcium phosphate supplementation (MCP). Compared with the CONT group, supplementation with CaCl increased the serum concentrations of osteocalcin and decreased the alkaline phosphatase (ALP) levels (p < 0.05); supplementation with MCP increased the average daily gain (ADG), serum concentrations of phosphorus (P) and osteocalcin, conception rate, and calving rate (p < 0.05), whereas it decreased the serum concentrations of hydroxyproline, ALP, and calcitonin (p < 0.05). Both CaCl and MCP supplementation had no effect on serum calcium (Ca) concentration. The ADG, conception rate, and calving rate were higher in the MCP group than in the CaCl group (p < 0.05), while the serum concentrations of hydroxyproline and calcitonin were lower (p < 0.05). It could be concluded that premating supplementation with MCP increased the body weight gain and subsequent conception and calving rate of grazing yaks. Supplementation with MCP had a positive effect on body condition and bone metabolism, thus providing a better estrous condition for grazing yak heifers, which could contribute to enhancing reproduction efficiency.

Shear seismic response analysis is critical for seismic design of immersed tunnels. According to the structural characters of immersed tunnels and shear dynamic response of their joints, a multibody dynamic model consisting of multi-rigid body, shear hinge, and viscous damping hinge is proposed for shear response analysis, in which the dynamic stiffness of the shear hinge is divided into two stages based on a threshold. Following the discrete time transfer matrix method for multibody system dynamics (MS-DT-TMM), the mechanical model and mathematical expression of each tunnel element is derived first and then assembled for the whole tunnel system. A solution procedure is proposed to solve the shear dynamic response of immersed tunnels using the proposed multibody system model. It is shown that the MS-DT-TMM has the same computational accuracy as the finite element method (FEM) and the modeling process is more efficient and flexible when compared to FEM. Although the MS-DT-TMM discussed herein is only applied to shear response analysis, it can easily be extended to analyze axial force and bending moment of immersed tunnels leading to a complete, rapid yet accurate enough seismic analysis of immersed tunnels suitable for engineering practices.

Crack extraction of solar panels has become a research focus in recent years. The cracks are small and hidden. In addition, there are particles of irregular shape and size on the surface of the polycrystalline solar panel, whose reflection position and direction are random. Therefore, there is a complex and uneven texture background on the solar panel image, which makes the crack extraction more difficult. In this paper, a crack extraction method combining image texture and morphological features is proposed. Firstly, the background texture and multi-scale details are suppressed by the linear filter and the Laplace pyramid decomposition method. Secondly, the edge can be extracted based on the modulus maximum method of the wavelet transform. Finally, cracks were extracted by using the improved Fuzzy C-means (FCM) clustering combining the morphological and texture features of the cracks. To make the extraction results more accurate and reasonable, an improved region growth algorithm is proposed to optimize the extraction results. All of the above research is closely centered on the accuracy and stability requirements of the solar cell crack detection, which is also the key point of this paper. The experimental results show that various improved or innovative algorithms proposed in this paper can accurately extract the position of cracks and obtain better extraction results. The detection results have good stability and can be faithful to the actual situation, which will promote the application of solar cells in more fields.