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251 results on '"Bach, Jean-Marie"'

51. Structural analysis of two HLA-DR-presented autoantigenic epitopes: crucial role of peripheral but not central peptide residues for T-cell receptor recognition

Author

De Oliveira, Daniel B., Harfouch-Hammoud, Elham, Otto, Heike, Papandreou, Nicolaos A., Stern, Lawrence J., Cohen, Hélène, Boehm, Bernhard O., Bach, Jean-Marie, Caillat-Zucman, Sophie, Walk, Tilmann, Jung, Günther, Eliopoulos, Elias, Papadopoulos, George K., and van Endert, Peter M.

Published
2000
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52. Immune phenotype and IgG characteristics of Neu5Gc and alpha-1-3-gal double knock-out pigs

Author

Salama, Apolline, Conchon, Sophie, Perota, Andrea, Martinet, Bernard, Judor, Jean-Paul, Evanno, Gwénaëlle, Bernardet, Stéphanie Le-Bas, Le Berre, Ludmilla, Hervouet, Jeremy, Minault, David, Concordet, Jean-Paul, Dugast, Emilie, Vanhove, Bernard, Abadie, Jérôme, Gaide, Nicolas, Lagutina, Irina, Duchi, Roberto, Lazzari, Giovanna, Floch, Robert, Duvaux, Odile, Toutain, Pierre-Louis, Maillasson, Mike, Sachs, David H., Gauthier, Olivier, Brouard, Sophie, Cozzi, Emanuele, Blancho, Gilles, Perreault, Hélène, Bach, Jean-Marie, Galli, Cesare, Soulillou, Jean-Paul, Institut National de la Santé et de la Recherche Médicale (INSERM), Société d'Accélération du Transfert de Technologies (SATT OUEST VALORISATION), Fondazione Avantea, Partenaires INRAE, Xenothera [Nantes, France], Centre National de la Recherche Scientifique (CNRS), Animaux modèles pour la recherche en oncologie comparée (AMaROC), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Massachusetts General Hospital [Boston], University Hospital of Padua, Consortium for Research in Organ Transplantation (CORIT), University of Manitoba, University of Manitoba [Winnipeg], Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Department of Veterinary Medical Sciences, University of Bologna, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

53. Increased late graft loss in kidney recipients with serum sickness disease following anti-thymocyte globulin induction: relation with an anti-NEU5GC response

Author

Salama, Apolline, Couvrat-Desvergnes, Gregoire, Le Berre, Ludmilla, Evanno, Gwenaelle, Viklicky, Ondrej, Hruba, Petra, Pavel Vesely, Guerif, Pierrick, Dejoie, Thomas, Nicot, Arnaud B., Bach, Jean-Marie, Brouard, Sophie, Castagnet, Stephanie, Giral, Magali, Harb, Jean, Foucher, Yohann, Lorent, Marine, Soulillou, Jean-Paul, Société d'Accélération du Transfert de Technologies (SATT OUEST VALORISATION), Institut National de la Santé et de la Recherche Médicale (INSERM), Transplant Center, Brno University of Technology [Brno] (BUT), Centre hospitalier universitaire de Nantes (CHU Nantes), Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Etablissement Français du Sang, Biostatistique, Recherche Clinique et Mesures Subjectives en Santé, and Université de Nantes (UN)

Subjects
[SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

54. Acute social stress-induced immunomodulation in pigs high and low responders to ACTH

Author

Bacou, Elodie, primary, Haurogné, Karine, additional, Mignot, Grégoire, additional, Allard, Marie, additional, De Beaurepaire, Laurence, additional, Marchand, Jordan, additional, Terenina, Elena, additional, Billon, Yvon, additional, Jacques, Julien, additional, Bach, Jean-Marie, additional, Mormède, Pierre, additional, Hervé, Julie, additional, and Lieubeau, Blandine, additional

Published
2017
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55. Neu5Gc and α1-3 GAL Xenoantigen Knockout Does Not Affect Glycemia Homeostasis and Insulin Secretion in Pigs

Author

Salama, Apolline, primary, Mosser, Mathilde, additional, Lévêque, Xavier, additional, Perota, Andrea, additional, Judor, Jean-Paul, additional, Danna, Corentin, additional, Pogu, Sylvie, additional, Mouré, Anne, additional, Jégou, Dominique, additional, Gaide, Nicolas, additional, Abadie, Jérôme, additional, Gauthier, Olivier, additional, Concordet, Jean-Paul, additional, Le Bas-Bernardet, Stéphanie, additional, Riochet, David, additional, Le Berre, Ludmilla, additional, Hervouet, Jérémy, additional, Minault, David, additional, Weiss, Pierre, additional, Guicheux, Jérôme, additional, Brouard, Sophie, additional, Bosch, Steffi, additional, Lagutina, Irina, additional, Duchi, Roberto, additional, Lazzari, Giovanna, additional, Cozzi, Emanuele, additional, Blancho, Gilles, additional, Conchon, Sophie, additional, Galli, Cesare, additional, Soulillou, Jean-Paul, additional, and Bach, Jean-Marie, additional

Published
2017
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57. Characteristics of IgGs Produced in Neu5Gc and alpha 1-3 Gal Double Knock-Out Pigs

Author

Salama, A., Conchon, S., Perota, A., Martinet, B., Judor, J. -P., Evanno, G., Bernardet, S. Le-Bas, Le Berre, L., Hervouet, J., Minault, D., Concordet, J. -P., Dugast, E., Vanhove, B., Abadie, J., Gaide, N., Lagutina, I., Duchi, R., Lazzari, G., Sachs, D., Gauthier, O., Brouard, S., Cozzi, E., Blancho, G., Perreault, H., Bach, Jean-Marie, Galli, C., Soulillou, J. -P., ProdInra, Migration, Université de Nantes (UN), Société d'Accélération du Transfert de Technologies (SATT OUEST VALORISATION), Fondazione Avantea, Partenaires INRAE, Structure et Instabilité des Génomes (STRING), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Acides nucléiques : dynamique, ciblage, et fonctions biologiques - Régulation et dynamique des génomes (ANDCFB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Animaux modèles pour la recherche en oncologie comparée (AMaROC), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Fondazione Avantea [Cremona, Italy], Massachusetts General Hospital, Massachusetts General Hospital [Boston], University Hospital of Padua, University of Manitoba, University of Manitoba [Winnipeg], Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and American Society of Transplant Surgeons (ASTS). Arlington, USA.

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

58. Extracellular hemoglobin combined with an O2‐generating material overcomes O2 limitation in the bioartificial pancreas.

Author

Mouré, Anne, Bacou, Elodie, Bosch, Steffi, Jegou, Dominique, Salama, Apolline, Riochet, David, Gauthier, Olivier, Blancho, Gilles, Soulillou, Jean‐Paul, Poncelet, Denis, Olmos, Eric, Bach, Jean‐Marie, and Mosser, Mathilde

Abstract

The bioartificial pancreas encapsulating pancreatic islets in immunoprotective hydrogel is a promising therapy for Type 1 diabetes. As pancreatic islets are highly metabolically active and exquisitely sensitive to hypoxia, maintaining O2 supply after transplantation remains a major challenge. In this study, we address the O2 limitation by combining silicone‐encapsulated CaO2 (silicone‐CaO2) to generate O2 with an extracellular hemoglobin O2‐carrier coencapsulated with islets. We showed that the hemoglobin improved by 37% the O2‐diffusivity through an alginate hydrogel and displayed antioxidant properties neutralizing deleterious reactive O2 species produced by silicone‐CaO2. While the hemoglobin alone failed to maintain alginate macroencapsulated neonate pig islets under hypoxia, silicone‐CaO2 alone or combined to the hemoglobin restored islet viability and insulin secretion and prevented proinflammatory metabolism (PTGS2 expression). Interestingly, the combination took the advantages of the two individual strategies, improved neonate pig islet viability and insulin secretion in normoxia, and VEGF secretion and PDK1 normalization in hypoxia. Moreover, we confirmed the specific benefits of the combination compared to silicone‐CaO2 alone on murine pseudo‐islet viability in normoxia and hypoxia. For the first time, our results show the interest of combining an O2 provider with hemoglobin as an effective strategy to overcome O2 limitations in tissue engineering. Mouré et al. adress the O2 limitation in a bioartificial pancreas, composed of neonate pig islets encapsulated in an alginate hydrogel, by combining an O2 generator (silicone‐CaO2) with an extracellular hemoglobin O2‐carrier. By increasing the O2 diffusivity in the alginate hydrogel and neutralising reactive O2 species produced by the silicone‐CaO2, the hemoglobin aims to improve the integration of the O2 generator into the device and the adaptation of the bioartificial pancreas to the varying O2 tensions encountered after transplantation. [ABSTRACT FROM AUTHOR]

Published
2019
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59. Metronidazole and Hydroxymetronidazole Central Nervous System Distribution: 1. Microdialysis Assessment of Brain Extracellular Fluid Concentrations in Patients with Acute Brain Injury

Author

Salama, Apolline, Fichou, Nolwenn, Allard, Marie, Dubreil, Laurence, De Beaurepaire, Laurence, Viel, Alexis, Jegou, Dominique, Bosch, Steffi, Bach, Jean-Marie, Frasca, Denis, Dahyot-Fizelier, Claire, Adier, Christophe, Mimoz, Olivier, Debaene, Bertrand, Couet, William, Marchand, Sandrine, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Conservatoire Botanique National Méditerranéen de Porquerolles, Conservatoire National Botanique, HEC Montréal (HEC Montréal), Developpement et Pathologie du Tissu Musculaire (DPTM), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Pharmacologie des anti-infectieux (PHAR), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Immuno-Endocrinologie Cellulaire et Moléculaire [Nantes] (IECM), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de la Recherche Agronomique (INRA), Université de Poitiers - Faculté de Médecine et de Pharmacie, Université de Poitiers, Centre hospitalier universitaire de Poitiers (CHU Poitiers), Service d'anesthésie réanimation [Poitiers], Développement et Pathologie du Tissu Musculaire (DPTM), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
Male, Microdialysis, [SDV]Life Sciences [q-bio], Central nervous system, Pharmacology, Blood–brain barrier, Cerebrospinal fluid, Anti-Infective Agents, In vivo, Metronidazole, Extracellular fluid, medicine, Humans, Distribution (pharmacology), Pharmacology (medical), Biotransformation, Chromatography, High Pressure Liquid, ComputingMilieux_MISCELLANEOUS, Aged, Chemistry, Brain, Extracellular Fluid, Middle Aged, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, 3. Good health, Infectious Diseases, medicine.anatomical_structure, Blood-Brain Barrier, Area Under Curve, Brain Injuries, Anesthesia, medicine.drug
Abstract

The distribution of metronidazole in the central nervous system has only been described based on cerebrospinal fluid data. However, extracellular fluid (ECF) concentrations may better predict its antimicrobial effect and/or side effects. We sought to explore by microdialysis brain ECF metronidazole distribution in patients with acute brain injury. Four brain-injured patients monitored by cerebral microdialysis received 500 mg of metronidazole over 0.5 h every 8 h. Brain dialysates and blood samples were collected at steady state over 8 h. Probe recoveries were evaluated by in vivo retrodialysis in each patient for metronidazole. Metronidazole and OH-metronidazole were assayed by high-pressure liquid chromatography, and a noncompartmental pharmacokinetic analysis was performed. Probe recovery was equal to 78.8% ± 1.3% for metronidazole in patients. Unbound brain metronidazole concentration-time curves were delayed compared to unbound plasma concentration-time curves but with a mean metronidazole unbound brain/plasma AUC 0–τ ratio equal to 102% ± 19% (ranging from 87 to 124%). The unbound plasma concentration-time profiles for OH-metronidazole were flat, with mean average steady-state concentrations equal to 4.0 ± 0.7 μg ml −1 . This microdialysis study describes the steady-state brain distribution of metronidazole in patients and confirms its extensive distribution.

Published
2014

60. Anti-EBOV GP IgGs Lacking α1-3-Galactose and Neu5Gc Prolong Survival and Decrease Blood Viral Load in EBOV-Infected Guinea Pigs

Author

Reynard, Olivier, primary, Jacquot, Frédéric, additional, Evanno, Gwénaëlle, additional, Mai, Hoa Le, additional, Salama, Apolline, additional, Martinet, Bernard, additional, Duvaux, Odile, additional, Bach, Jean-Marie, additional, Conchon, Sophie, additional, Judor, Jean-Paul, additional, Perota, Andrea, additional, Lagutina, Irina, additional, Duchi, Roberto, additional, Lazzari, Giovanna, additional, Le Berre, Ludmilla, additional, Perreault, Hélène, additional, Lheriteau, Elsa, additional, Raoul, Hervé, additional, Volchkov, Viktor, additional, Galli, Cesare, additional, and Soulillou, Jean-Paul, additional

Published
2016
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61. Carbon monoxide-treated dendritic cells decrease β1-integrin induction on CD8 + T cells and protect from type 1 diabetes

Author

Simon, Thomas, Pogu, Sylvie, Tardif, Virginie, Rigaud, Kevin, Rémy, Severine, Piaggio, Eliane, Bach, Jean-Marie, Anegon, Ignacio, Blancou, Philippe, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Genetic and Cellular Engineering in Immunology and Regenerative Medicine (Team 2 - U1064 Inserm - CRTI), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Immunologie - Immunopathologie - Immunothérapie (I3), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Fondation Centaure, Fondation Progreffe. Grant Number: 5–2010‐640., Région Pays de la Loire through the core facility Research and Development for Clinical Transfer., Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Immunologie - Immunopathologie - Immunothérapie [CHU Pitié Salpêtrière] (I3), CHU Charles Foix [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Le Bihan, Sylvie, Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Integrin beta1, Autoreactive CD8+ T cells, Diabetes, Down-Regulation, β1-integrin, Mice, Transgenic, CD8-Positive T-Lymphocytes, Autoantigens, Dendritic cells, Coculture Techniques, Peptide Fragments, Disease Models, Animal, Mice, Diabetes Mellitus, Type 1, Cell Movement, Insulin-Secreting Cells, Immune Tolerance, Animals, Humans, Carbon monoxide, Pancreas, Cells, Cultured, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Carbon monoxide (CO) treatment improves pathogenic outcome of autoimmune diseases by promoting tolerance. However, the mechanism behind this protective tolerance is not yet defined. Here, we show in a transgenic mouse model for autoimmune diabetes that ex vivo gaseous CO (gCO)-treated DCs loaded with pancreatic β-cell peptides protect mice from disease. This protection is peptide-restricted, independent of IL-10 secretion by DCs and of CD4 + T cells. Although no differences were observed in autoreactive CD8 + T-cell function from gCO-treated versus untreated DC-immunized groups, gCO-treated DCs strongly inhibited accumulation of autoreactive CD8 + T cells in the pancreas. Interestingly , induction of β1-integrin was curtailed when CD8 + T cells were primed with gCO-treated DCs, and the capacity of these CD8 + T cells to lyse isolated islet was dramatically impaired. Thus, immunotherapy using CO-treated DCs appears to be an original strategy to control autoimmune disease.

Published
2013

64. Myeloid derived suppressor cells recruited by cyclophosphamide in the spleen of non-obese diabetic mouse might participate to diabetes acceleration through their further migration and differentiation into the pancreas

Author

Caquard, Marylène, Ferret-Bernard, Stéphanie, Haurogne, Karine, Ouary, Michèle, Allard, Marie, Bach, Jean-Marie, Lieubeau-Teillet, Blandine, ESIM - Déterminants Sociaux de la Santé et du Recours aux Soins (DS3), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), UMR 707A IECM, Ecole Nationale Vétérinaire de Nantes, Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

absent

Published
2008

66. Effet de la protéine pdx1-vp16 sur la différenciation d'une lignée de cellules souches hépatiques en cellules insulino-sécrétrices

Author

Cuvelier Delisle, Juliette, Bosch, Steffi, Martignat, Lionel, Bach, Jean-Marie, Louzier, Vanessa, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
DIFFÉRENCIATION PANCRÉATIQUE ENDOCRINE, [SDV]Life Sciences [q-bio], DIABÈTE TYPE 1
Published
2007

67. Easy and rapid method of zygosity determination in transgenic mice by SYBR (R) Green real-time quantitative PCR with a simple data analysis

Author

Haurogne, Karine, Bach, Jean-Marie, Lieubeau-Teillet, Blandine, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes

Subjects
TRANSGENIC MICE, [SDV.GEN]Life Sciences [q-bio]/Genetics, REAL-TIME QUANTITATIVE PCR, ZYGOSITY, SYBR (R) GREEN
Abstract

International audience; Establishment and maintenance of transgenic mouse strains require being able to distinguish homozygous from heterozygous animals. To date, the developed real-time quantitative PCR techniques are often complicated, time-consuming and expensive. Here, we propose a very easy and rapid method with a simple data analysis to determine zygosity in transgenic mice. We show that the real-time quantitative PCR using SYBR (R) Green fluorescent dye can be applied to discriminate two-fold differences in copy numbers of the transgene. Our procedure has to fit only three simple requirements: (1) to design primers capable of detecting one Ct difference for two-fold differences in DNA amounts (2) to measure genomic DNA concentrations accurately and (3) to have a reference animal of known zygosity in each run. Then, if the Ct values for the control gene are similar in all samples, we are able to compare directly the Ct values for the transgene in every sample, and so, to deduce the zygosity status of each mouse relative to the reference animal. This method is really simple and reliable, and it may be valuable as a rapid screening tool for zygosity status in transgenic animals.

Published
2007

69. CD8+ T-Cell Responses Identify {beta}-Cell Autoimmunity in Human Type 1 Diabetes Diabetes

Author

Mallone, Roberto, Martinuzzi, Emanuela, Blancou, Philippe, Novelli, Giulia, Afonso, Georgia, Dolz, Manuel, Bruno, Graziella, Chaillous, Lucy, Chatenoud, Lucienne, Bach, Jean-Marie, Van Endert, Peter, Diabète de Type 1 : mécanismes et traitements immunologiques, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine, Université de Ngozi, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Universita di Torino, Clinique d'Endocrinologie, Institut du Thorax-Centre hospitalier universitaire de Nantes (CHU Nantes), ProdInra, Migration, and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], GLUTAMIC-ACID DECARBOXYLASE, MONOCLONAL-ANTIBODY, IDENTIFICATION, [SDV]Life Sciences [q-bio], ONSET, IMMUNE-RESPONSE, ASSAYS, PERIPHERAL-BLOOD, NOD MICE, INSULIN, DISEASE
Abstract

International audience; Despite the understanding that type 1 diabetes pathogenesis is mediated by T-cells, detection of these rare lymphocytes remains largely elusive. Suitable T-cell assays are highly needed, since they could offer preclinical diagnoses and immune surrogate end points for clinical trials. Although CD4+ T-cell assays have met with limited success, CD8+ T-cells are increasingly recognized as key actors in the diabetes of the NOD mouse. CD8+ T-cells are likely to play a role also in humans and may provide new markers of β-cell autoimmunity. Taking advantage of a panel of HLA-A2–restricted β-cell epitopes derived from preproinsulin, GAD, and islet glucose-6-phosphatase catalytic subunit-related protein (IGRP), we have implemented an islet-specific CD8+ T-cell interferon-γ enzyme-linked immunospot (ISL8Spot) assay. The ISL8Spot assay is capable of detecting and quantifying β-cell–reactive CD8+ T-cells directly ex vivo, without any preliminary expansion, using either fresh or frozen samples. Positive ISL8Spot responses separate new-onset diabetic and healthy samples with high accuracy (86% sensitivity, 91% specificity), using as few as five immunodominant epitopes. Moreover, sensitivity reaches 100% when the ISL8Spot assay is complemented by antibody determinations. Combination of CD8+ T-cell measurements with immune intervention strategies may open new avenues toward type 1 diabetes prediction and prevention.

Published
2007

70. Caractérisation des cellules dendritiques canines différenciées à partir des monocytes du sang périphérique

Author

Ibisch, Catherine, Lieubeau, Blandine, Pradal, Gilbert, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Université de Nantes (UN), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
ULTRASTRUCTURE, CELLULE DENDRITIQUE, [SDV]Life Sciences [q-bio], CULTURE CELLULAIRE, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2006

71. HLA-A2 restricted islet-reactive CD8+ T-cells in type 1 diabetes

Author

Blancou, Philippe, Sévère, Sabine, Pogu, Sylvie, Haurogne, Karine, Van Endert, PM, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), and Inconnu

Subjects
[SDV]Life Sciences [q-bio]
Published
2006

72. Lymphocytic insulitis in a dog with juvenile diabetes mellitus

Author

Jouvion, Gregory, Abadie, Jérôme, Bach, Jean-Marie, Roux, F, Miclard, J, Deschamps, Jack-Yves, Guigand, Lydie, Saï, Pierre, WYERS, Monique, Inconnu, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Développement et Pathologie du Tissu Musculaire (DPTM), and Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes

Subjects
[SDV]Life Sciences [q-bio]
Published
2006

73. Functional canine dendritic cells generated in vitro from peripheral blood mononuclear cells (PBMC) : Structural and functional characterization

Author

Ibisch, Catherine, Bach, Jean-Marie, Lieubeau, Blandine, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], IMMUNOLOGIE, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2005

74. CD8+ T Lymphocytes Specific to GAD65 could mediate autoimmune diabetes in NOD mouse

Author

Sévère, Sabine, Gauvrit, Anne, Vu, Anh Tuan, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
LYMPHOCYTE, AUTOIMMUNE DIABETE, [SDV]Life Sciences [q-bio], IMMUNOLOGIE
Published
2005

75. Myeloid suppressor cell (MSC) deficiencies in non-obese diabetic (NOD) mice: a role in type 1 diabetes pathogenesis ?

Author

Ferret-Bernard, Stéphanie, Allard, Marie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
obésité, [SDV]Life Sciences [q-bio], souris, lymphocyte, MYELOID SUPPRESSOR CELL, mus musculus, diabète
Abstract

absent

Published
2004

76. Identification and characterization of diabetogenic CD8+ T-cells specific of 65 kDa glutamic acid decarboxylase (GAD65) using DNA immunization in non-obese diabetic (NOD) mouse

Author

Sévère, Sabine, Gauvrit, Anne, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
NON OBESE DIABETIC, PLASMID IMMUNIZATION, GLUTAMIC ACID DECARBOXYLASE, [SDV]Life Sciences [q-bio]
Published
2004

79. Ultrastructural markers expressed in cultured canine dendritic cells and laminated bodies found in Langerhans cells : similarities and differences

Author

Ibisch, Catherine, Lieubeau, Blandine, Pradal, Gilbert, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Université de Nantes (UN), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes

Subjects
LYMPHOCYTE, LANGERHANS CELL, [SDV]Life Sciences [q-bio], DENDRITIC CELL, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

80. Mise au point d’un modèle de diabète insulinodépendant chez le chien

Author

Joussemet, Béatrice, Saï, Pierre, Bach, Jean-Marie, Inconnu, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio]
Published
2004

82. Inhibitory macrophage deficiency in NOD mice : a role in type 1 diabetes pathogenesis ?

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
NON OBESE DIABETIC, [SDV]Life Sciences [q-bio], MACROPHAGE, DENDRITIC CELL
Abstract

absent

Published
2003

83. Déficience des iMacs chez la souris NOD : implication dans le diabète autoimmun ?

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], DIABÈTE TYPE 1
Abstract

absent

Published
2003

85. GAD peptide vaccines for T1DM : not just a blueprint ?

Author

Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), and Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)

Subjects
DIABETE TYPE 1, AUTOIMMUNE DISEASE, NON OBESE DIABETE, [SDV]Life Sciences [q-bio]
Abstract

DMCCAD Stockholm Sweden June 2003

Published
2003

86. Génération de iMacs in vitro à partir de progéniteurs hématopoïétique de souris

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
immunosuppression, [SDV]Life Sciences [q-bio], iMacs, progenitors
Abstract

Notre étude précise l'origine myéloïde des iMacs et fournit une méthode relativement simple pour générer in vitro des iMacs fonctionnels à partir de progéniteurs hématopoïétiques murins.

Published
2002

87. Déficience des iMacs chez la souris Nod : implication dans le diabète autoimmun ?

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

Les iMacs de souris NOD présentent des déficiences de maturation et de fonction. Ces anomalies pourraient être impliquées dans la progression du TID induit par CyP.

Published
2002

88. iMacs deficiency in NOD mice: implication in cyclophosphamide-induced diabetes?

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

Background and aims: The role of dendritic cells and macrophages in pancreatic beta-cell destruction in type I diabetes, remains unclear. NOD mice displays functional dendritic cell abnormalities which could be involved in self-tolerance break-down to beta-cell antigens. A peculiar set of macrophages, CD11b+Gr-1+ inhibitory macrophages (iMacs), is implicated in profound depression of CD8 T-cell functions sometimes observed in tumor growth, treatment with cyclophosphamide (CyP) and overwhelming infections. iMacs could trigger apoptotic death of T-lymphocytes through a secretion of NO, and may be involved in the control of T-cell expansion. The aim of our study was to characterize the phenotype of iMacs in NOD mice and to investigate their possible role in diabetes pathogenesis.Materials and methods: iMacs were obtained either (1) after a 6 day-culture of lin- c-kit+ hematopoietic progenitor cells (HPC) with GM-CSF, SCF and TNF-alpha or (2) by purification from spleens of mice treated with low doses of CyP (100mg/kg, three times at three-day intervals).Results: Compared to HPC from BALB/c and C57BL/6 mice, NOD-HPC were unable to differentiate into iMacs (p

Published
2002

89. Caractérisation d'un défaut de maturation et d'une déficience fonctionnelle des iMacs chez la souris NOD : implication dans la pathogénie du diabète de type 1 ?

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes

Subjects
[SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract

absent

Published
2002

90. Défaut de maturation et déficience fonctionnelle des iMACS chez la souris NOD : implication dans la pathogénie du diabète de type 1 ?

Author

Ferret-Bernard, Stéphanie, Saï, Pierre, Bach, Jean-Marie, Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
DIABETE DE TYPE 1, [SDV]Life Sciences [q-bio], Tolérance périphérique, cellule, souris, mus musculus, iMacs
Abstract

absent

Published
2002

91. Carbon monoxide decreases endosome-lysosome fusion and inhibits soluble antigen presentation by dendritic cells to T cells.

Author

Tardif, Virginie, primary, Riquelme, Sebastián A., additional, Remy, Séverine, additional, Carreño, Leandro J., additional, Cortés, Claudia M., additional, Simon, Thomas, additional, Hill, Marcelo, additional, Louvet, Cédric, additional, Riedel, Claudia A., additional, Blancou, Philippe, additional, Bach, Jean-Marie, additional, Chauveau, Christine, additional, Bueno, Susan M., additional, Anegon, Ignacio, additional, and Kalergis, Alexis M., additional

Published
2013
Full Text
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97. Carbon Monoxide Inhibits TLR-Induced Dendritic Cell Immunogenicity

Author

Rémy, Séverine, primary, Blancou, Philippe, additional, Tesson, Laurent, additional, Tardif, Virginie, additional, Brion, Régis, additional, Royer, Pierre Joseph, additional, Motterlini, Roberto, additional, Foresti, Roberta, additional, Painchaut, Marion, additional, Pogu, Sylvie, additional, Gregoire, Marc, additional, Bach, Jean Marie, additional, Anegon, Ignacio, additional, and Chauveau, Christine, additional

Published
2009
Full Text
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