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51. Mora Romagnola, le attuali esperienze sono positive

Author

ZAMBONELLI, PAOLO, BIGI, DANIELE, Zambonelli P., and Bigi D

Subjects
RAZZE AUTOCTONE, SUINO, MORA ROMAGNOLA, CONSANGUINEITÀ
Abstract

Analisi aggiornata dell'allevamento della razza suina autoctona italiana Mora Romagnola. Indicazioni sulle strategie selettive necessarie per mantenere un accettabile livello di consanguineità in questa razza a ridotta consistenza numerica.

Published
2006

52. Maternal lineages of Ventasso Horse detected by mitochondrial D-loop sequence analysis

Author

ZAMBONELLI, PAOLO, BIGI, DANIELE, Zambonelli P., and Bigi D.

Subjects
GENETIC DIVERSITY, ITALIAN HORSE BREEDS, MITOCHONDRIAL DNA
Abstract

The Ventasso Horse (VH) is a native Italian breed selected for saddle, and its name comes from Mount Ventasso, located in the province of Reggio Emilia. According to FAO, the small number of the VH population, of about 300 individuals, place the VH in the list of endangered breeds. During the XX Century, Maremmano and Lipizzan stallions were used to improve VH but no information about the introduction of mares is reported. The sequence analysis of hypervariable region of mitochondrial DNA D-loop is the ideal tools to detect maternal lineages present in this breed today. Samples of 46 VH individuals having different mothers were collected. Total DNA was extracted from hair roots. A 752 nt fragment of the D-loop region was amplified (nucleotides 15,339-16,090 of the reference sequence X79547). The amplified product was sequenced on both strands obtaining a 667 nt sequence (15,372-16,039). The sequences were aligned with MultAlin software. A total of 43 mutation spots, of which 20 showed high frequency, were detected. On the whole, 21 haplotypes were observed. VH sequences were compared with 480 horse mitochondrial D-loop DNA sequences retrieved from GenBank, representing a subset of 1,249 sequences found (March, 2006). Sequences longer than 300 nt where chosen. We also decided to include sequences belonging to all breeds present in the database. Furthermore, an Equus asinus sequence (accession No X97337) was included as outgroup for phylogeny studies. On the whole 99 horse breeds were compared with VH, using a sequence of 254 nt (15,487-15,740) common to all considered samples. With this shorter sequence only 14 out of 20 of the most represented mutation spots were considered. Multiple alignments and phylogeny construction were carried out with Mega 3.1 software. Dendrograms were obtained using Neighbour-Joining procedure with 1,000 bootstrap resampling. This analysis reveals a group of 19 out of 46 VH samples showing an unique haplotype not found in the other sequences considered.

Published
2006

53. Towards the integration of SNP allele frequency estimation and microsatellite data in a selective milk DNA pooling experiment designed to identify QTL in dairy cattle

Author

FONTANESI, LUCA, SCOTTI, EMILIO, TAZZOLI, MARCO, ZAMBONELLI, PAOLO, DALL'OLIO, STEFANIA, BIGI, DANIELE, DAVOLI, ROBERTA, RUSSO, VINCENZO, Dolezal M., Lipkin E., Soller M., Fontanesi L., Scotti E., Tazzoli M., Zambonelli P., Dall'Olio S., Bigi D., Dolezal M., Lipkin E., Soller M., Davoli R., and Russo V.

Subjects
ALLELE FREQUENCY, SNP, QTL MAP, SELECTIVE MILK DNA POOLING, ITALIAN HOLSTEIN FRIESIAN BREED
Published
2006

54. ANALYSIS OF BOVINE CHROMOSOME 20 FOR QTL AFFECTING MILK PRODUCTION AND REPRODUCTIVE TRAITS APPLYING A SELECTIVE MILK DNA POOLING STRATEGY IN THE ITALIAN HOLSTEIN POPULATION

Author

FONTANESI, LUCA, RUSSO, VINCENZO, SCOTTI, EMILIO, ZAMBONELLI, PAOLO, DALL'OLIO, STEFANIA, BIGI, DANIELE, DAVOLI, ROBERTA, Canavesi F., Lipkin E., Soller M., Fontanesi L., Russo V., Scotti E., Zambonelli P., Dall'Olio S., Bigi D., Canavesi F., Lipkin E., Soller M., and Davoli R.

Subjects
GROWTH HORMONE RECEPTOR, QTL, MICROSATELLITES, food and beverages, ITALIAN HOLSTEIN FRIESIAN BREED, BOVINE CHROMOSOME 20
Abstract

Bovine chromosome 20 was screened to identify QTL for milk yield (MY), protein percentage (PP), protein yield (PY), fat yield (FY), fat percentage (FP), milk somatic cell count (MSCC), calving difficulties and perinatal mortality using a daughter design and a selective milk DNA pooling approach in the Italian Holstein breed. The results may confirm, even if indirectly, the effect of the growth hormone receptor (GHR) F279Y mutation on PP, FP and correlated traits. Other two putative QTL affecting PP, two affecting MY (as well as PY and FY) and MSCC, and two affecting reproductive traits have been identified.

Published
2006

55. Isolation of differentially expressed genes related with ham saltino loss in Italian heavy pig

Author

RUSSO, VINCENZO, BIGI, DANIELE, ZAMBONELLI, PAOLO, Colombo M., Russo V., Bigi D., Zambonelli P., and Colombo M.

Subjects
PIG, DIFFERENTIAL DISPLAY, SALTING LOSS, DNA, META QUALITY
Abstract

We used Fluoro Differential Display (FDD) technique to search candidate genes for salting loss (SL) ham in Italian heavy pig. A group of 277 Large White pigs, 183 females and 94 castrated males were slaughtered. Four unrelated animals (2 females and 2 castrated males) with the lowest SL index, and 4 with the highest SL index were selected. Total RNA was extracted from a sample of semitendinosus muscle of each animal and collected in two pools, one for each group of pigs. The FDD protocol, utilized to identify differentially expressed genes between the two groups, gave fingerprints composed of about 50 well distinct bands in the size range of 200-1500 bp. On the whole about 5000 different cDNAs fragments were separated and analysed. Among these bands, we found 12 strongly differentially–expressed bands, always over expressed on the pool with the highest weight loss. The DNA extracted from the bands was sequenced and similarity was found to 6 known genes, to 4 gene with an uncharacterized function, and to one L1 interspersed element. Four of these genes, Sarcolipin (SLN), Triadin (TRDN), Titin (TTN) and Down syndrome critical region 1 (DSCR1) are mostly expressed in the skeletal and cardiac muscle and could be involved in the determination of meat quality in pigs. Before to consider these genes for association studies, these results needs to be validated by an alternative method of evaluation of gene expression.

Published
2005

56. Razze zootecniche in pericolo di estinzione: la bovina Reggiana

Author

BIGI, DANIELE and Bigi D.

Subjects
BOVINO, RAZZA REGGIANA, PARMIGIANO REGGIANO, LATTE
Abstract

Analisi aggiornata riguardante lo stato dell'allevamento ella bovina Reggiana, razza autoctona italiana utilizzata per la produzione del Parmigiano Reggiano.

Published
2005

57. The BovMAS: Analysis of BTA14 for QTL influencing milk yeld, milk composition and health traits in the Italian Holstein-Friesian cattle breed

Author

FONTANESI, LUCA, SCOTTI, EMILIO, ZAMBONELLI, PAOLO, DALL'OLIO, STEFANIA, BIGI, DANIELE, DAVOLI, ROBERTA, RUSSO, VINCENZO, Pecorari D., Dolezal M., Lipkin E., Soller M., Fontanesi L., Scotti E., Pecorari D., Dolezal M., Zambonelli P., Dall’Olio S., Bigi D., Davoli R., Lipkin E., Soller M., and Russo V.

Subjects
MILK YELD, QTL, food and beverages, BOVINE, DNA, reproductive and urinary physiology, MILK PROTEIN
Abstract

Bovine chromosome 14 (BTA14) was scanned for QTL affecting milk yield, protein percentage and yield, fat percentage and yield and somatic cell count. Selective milk DNA pooling strategy was applied in a daughter design for eight Holstein-Friesian sires. For each sire-trait combination, milk pools were constructed comprising ~200 best or worst daughters ranked by their EBV or DYD. The sires were genotyped for 16 microsatellites covering BTA14, for the A232K and VNTR mutations at the DGAT1 locus and for a SNP of the tyroglobulin gene. The milk pools were genotyped at the microsatellites heterozygous in their sire. Shadow corrected estimates of allele frequencies were calculated and differences in sire allele frequencies between high and low pools were computed. An adjusted false discovery rate was applied to calculate chromosome-wide significant levels and the data were analysed using an approximate interval mapping procedure. The results indicate the presence of one/more QTL at the centromeric end affecting most traits and one/more putative QTL in the middle-distal part influencing mainly protein percentage.

Published
2005

58. The BovMAS Consortium: Mapping QTL for milk yield and protein percentage in Italian Holstein-Friesian dairy cattle by selective DNA pooling of milk samples

Author

RUSSO, VINCENZO, FONTANESI, LUCA, ZAMBONELLI, PAOLO, DALL'OLIO, STEFANIA, BIGI, DANIELE, SCOTTI, EMILIO, PECORARI, DANIELA, DAVOLI, ROBERTA, Blasi M., Motovalian M., Lanza A., Canavesi F., Lipkin E., Soller M., Russo V., Fontanesi L., Zambonelli P., Dall'Olio S., Bigi D., Scotti E., Pecorari D., Blasi M., Motovalian M., Lanza A., Canavesi F., Lipkin E., Soller M., and Davoli R.

Subjects
SELECTIVE DNA POOLING, QTL, MILK YELD, PROTEIN PERCENTAGE, ITALIAN HOLSTEIN FRIESIAN, DAIRY CATTLE
Published
2004

63. Multilocus Detection of Wolf x Dog Hybridization in Italy, and Guidelines for Marker Selection.

Author

Randi, Ettore, Hulva, Pavel, Fabbri, Elena, Galaverni, Marco, Galov, Ana, Kusak, Josip, Bigi, Daniele, Bolfíková, Barbora Černá, Smetanová, Milena, and Caniglia, Romolo

Subjects
WOLFDOGS, DOG breeding, BIOMARKERS, ENDANGERED species, MITOCHONDRIAL DNA, Y chromosome
Abstract

Hybridization and introgression can impact the evolution of natural populations. Several wild canid species hybridize in nature, sometimes originating new taxa. However, hybridization with free-ranging dogs is threatening the genetic integrity of grey wolf populations (Canis lupus), or even the survival of endangered species (e.g., the Ethiopian wolf C. simensis). Efficient molecular tools to assess hybridization rates are essential in wolf conservation strategies. We evaluated the power of biparental and uniparental markers (39 autosomal and 4 Y-linked microsatellites, a melanistic deletion at the β-defensin CBD103 gene, the hypervariable domain of the mtDNA control-region) to identify the multilocus admixture patterns in wolf x dog hybrids. We used empirical data from 2 hybrid groups with different histories: 30 presumptive natural hybrids from Italy and 73 Czechoslovakian wolfdogs of known hybrid origin, as well as simulated data. We assessed the efficiency of various marker combinations and reference samples in admixture analyses using 69 dogs of different breeds and 99 wolves from Italy, Balkans and Carpathian Mountains. Results confirmed the occurrence of hybrids in Italy, some of them showing anomalous phenotypic traits and exogenous mtDNA or Y-chromosome introgression. Hybridization was mostly attributable to village dogs and not strictly patrilineal. The melanistic β-defensin deletion was found only in Italian dogs and in putative hybrids. The 24 most divergent microsatellites (largest wolf-dog FST values) were equally or more informative than the entire panel of 39 loci. A smaller panel of 12 microsatellites increased risks to identify false admixed individuals. The frequency of F1 and F2 was lower than backcrosses or introgressed individuals, suggesting hybridization already occurred some generations in the past, during early phases of wolf expansion from their historical core areas. Empirical and simulated data indicated the identification of the past generation backcrosses is always uncertain, and a larger number of ancestry-informative markers is needed. [ABSTRACT FROM AUTHOR]

Published
2014
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64. The GENETPIG database: a tool for comparative mapping in pig (Sus scrofa)

Author

Karsenty, Emmanuelle, Barillot, Emmanuel, Tosser-Klopp, Gwenola, Lahbib-Mansais, Yvette, Milan, Denis, Hatey, François, Cirera, Susanna, Sawera, Milena, Jørgensen, Claus B., Chowdhary, Bhanu, Fredholm, Merete, Wimmers, Klaus, Ponsuksili, Siriluck, Davoli, Roberta, Fontanesi, Luca, Braglia, Silvia, Zambonelli, Paolo, Bigi, Daniele, Neuenschwander, Stefan, Gellin, Joël, Karsenty, Emmanuelle, Barillot, Emmanuel, Tosser-Klopp, Gwenola, Lahbib-Mansais, Yvette, Milan, Denis, Hatey, François, Cirera, Susanna, Sawera, Milena, Jørgensen, Claus B., Chowdhary, Bhanu, Fredholm, Merete, Wimmers, Klaus, Ponsuksili, Siriluck, Davoli, Roberta, Fontanesi, Luca, Braglia, Silvia, Zambonelli, Paolo, Bigi, Daniele, Neuenschwander, Stefan, and Gellin, Joël

Abstract

The GENETPIG database has been established for storing and disseminating the results of the European project: ‘GENETPIG: identification of genes controlling economic traits in pig'. The partners of this project have mapped about 630 porcine and human ESTs onto the pig genome. The database collects the mapping results and links them to other sources of mapping data; this includes pig maps as well as available comparative mapping information. Functional annotation of the mapped ESTs is also given when a significant similarity to cognate genes was established. The database is accessible for consultation via the Internet at http://www.infobiogen.fr/services/Genetpig

65. 'Improving text comprehension in ESL learners: a multichannel approach'

Author

Daniele Bigi, Federica Ferrari, Mariangela Picciuolo, Ferrari, Federica, Picciuolo, Mariangela, and Bigi, Daniele

Subjects
050101 languages & linguistics, Linguistics and Language, 05 social sciences, Perspective (graphical), 050301 education, Multisensory technique, Subject (documents), Language and Linguistics, Linguistics, Education, Text comprehension, Multichannel approach, ComputerApplications_MISCELLANEOUS, reading strategie, 0501 psychology and cognitive sciences, Language awarene, Second language learning, Psychology, 0503 education, On Language
Abstract

Purpose: This paper is a proposal for improving ESL text comprehension by merging a multimodal perspective on language (Kress, 2005) with an integrated psychological (or ‘metacognitive’) approach to language learning and awareness (Fairclough, 1992, p. 1). Design: A multichannel approach is presented, based on two strategies: a main ‘multichannel’ strategy for reading the text, which focuses on the assessment of the visual, auditory, cognitive and kinaesthetic/emotional channels and relative functions, and a corollary one, specifically dedicated to implementing the cognitive function. Methodology/ Approach: The approach has been extensively applied in university-level English classes (Reggio Emilia, Parma, Bologna University) using a ‘multichannel test’ based on students’ feedback to explore the effectiveness of the method. In this paper, a case study is offered: data have been collected with specific reference to the University of Bologna, A. Y. 2017/18 and 2018/2019. Originality/Value: Evidence suggests that further and diversified applications promise an empowerment of the approach and of its impact on ELT as well as on students’ awareness and satisfaction.

Published
2022

66. Analysis of Polycerate Mutants Reveals the Evolutionary Co-option of HOXD1 for Horn Patterning in Bovidae

Author

Daniele Bigi, Fiona Menzi, Slim Ben Jemaa, Marie-Christine Deloche, Aurélien Capitan, Johannes A. Lenstra, Marina Naval-Sanchez, Ivica Medugorac, Nathalie Hirter, Gwenola Tosser-Klopp, Diane Esquerre, Coralie M. Reich, Julia M. Paris, Rose-Marie Arbogast, Amandine Blin, Abdelhak Boukadiri, Aurélie Hintermann, Julie Rivière, Denis Duboule, Raphaël Cornette, Cécile Donnadieu, Marie-Dominique Wandhammer, Gjoko Bunevski, Louisa Gidney, Michael Stache, Isabelle Palhiere, Renate Schafberg, James Kijas, Claude Guintard, Joséphine Lesur, Jozsef Zakany, Rachel Rupp, Noelle E. Cockett, John Hedges, Ashleigh Haruda, Philippe Bardou, Olivier Putelat, Tracy Hadfield, Alain Pinton, Ockert Greyvenstein, Aurélie Allais-Bonnet, Este Van Marle-Koster, Eric Pailhoux, Coralie Danchin-Burge, David G. Riley, Cécile Grohs, Benjamin J. Hayes, Cord Drögemüller, Allice, Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Martin-Luther-University Halle-Wittenberg, Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Geneva [Switzerland], University of Bologna, Ludwig Maximilian University [Munich] (LMU), Archéologie d'Alsace, Archéologie et histoire ancienne : Méditerranée - Europe (ARCHIMEDE), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Texas A&M University [College Station], Utah State University (USU), Université de Carthage - University of Carthage, Ss. Cyril and Methodius University in Skopje, University of Bern, Manx Text, Manx Loaghtan Sheep Breeders (MLSBG), Utrecht University [Utrecht], Rent a Peasant, Archéozoologie, archéobotanique : sociétés, pratiques et environnements (AASPE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Musée Zoologique de Strasbourg, Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Acquisition et Analyse de Données pour l'Histoire naturelle (2AD), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de l'élevage (IDELE), Agriculture Victoria (AgriBio), Tshwane University of Technology [Pretoria] (TUT), Queensland Alliance for Agriculture and Food Innovation (QAAFI), University of Queensland [Brisbane], CSIRO Agriculture and Food (CSIRO), Ecole Polytechnique Fédérale de Lausanne (EPFL), Collège de France (CdF (institution)), Allais-Bonnet, Aurélie, Hintermann, Aurélie, Deloche, Marie-Christine, Cornette, Raphaël, Bardou, Philippe, Naval-Sanchez, Marina, Pinton, Alain, Haruda, Ashleigh, Grohs, Cécile, Zakany, Jozsef, Bigi, Daniele, Medugorac, Ivica, Putelat, Olivier, Greyvenstein, Ockert, Hadfield, Tracy, Jemaa, Slim Ben, Bunevski, Gjoko, Menzi, Fiona, Hirter, Nathalie, Paris, Julia M, Hedges, John, Palhiere, Isabelle, Rupp, Rachel, Lenstra, Johannes A, Gidney, Louisa, Lesur, Joséphine, Schafberg, Renate, Stache, Michael, Wandhammer, Marie-Dominique, Arbogast, Rose-Marie, Guintard, Claude, Blin, Amandine, Boukadiri, Abdelhak, Rivière, Julie, Esquerré, Diane, Donnadieu, Cécile, Danchin-Burge, Coralie, Reich, Coralie M, Riley, David G, Marle-Koster, Este van, Cockett, Noelle, Hayes, Benjamin J, Drögemüller, Cord, Kijas, Jame, Pailhoux, Eric, Tosser-Klopp, Gwenola, Duboule, Deni, Capitan, Aurélien, École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), École nationale vétérinaire - Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Université de Genève = University of Geneva (UNIGE), University of Bologna/Università di Bologna, Ss. Cyril and Methodius University in Skopje (UKIM), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), and Collège de France - Chaire internationale Évolution des génomes et développement

Subjects
0106 biological sciences, Male, co-option, translocation, AcademicSubjects/SCI01180, regulatory mutation, 01 natural sciences, Hox genes, Hox gene, genes, 610 Medicine & health, Bilateria, time, Horns, 0303 health sciences, biology, Ecology, 630 Agriculture, Goats, [SDV.BDD.EO] Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Gene Expression Regulation, Developmental, alignment, Morphogenetic field, Biological Evolution, [SDV.BDD.MOR] Life Sciences [q-bio]/Development Biology/Morphogenesis, 590 Animals (Zoology), Haploinsufficiency, transcription, Biometry, Evolution, growth, Locus (genetics), Mice, Transgenic, [SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics, 010603 evolutionary biology, 03 medical and health sciences, goat and sheep genomics, Behavior and Systematics, framework, Genetics, Animals, Allele, Gene, Molecular Biology, Discoveries, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Homeodomain Proteins, locus, Sheep, Horn (anatomy), AcademicSubjects/SCI01130, [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis, sequence, biology.organism_classification, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Evolutionary biology, Mutation, genome-wide association, 570 Life sciences, goat and sheep genomic
Abstract

In the course of evolution, pecorans (i.e., higher ruminants) developed a remarkable diversity of osseous cranial appendages, collectively referred to as “headgear,” which likely share the same origin and genetic basis. However, the nature and function of the genetic determinants underlying their number and position remain elusive. Jacob and other rare populations of sheep and goats are characterized by polyceraty, the presence of more than two horns. Here, we characterize distinct POLYCERATE alleles in each species, both associated with defective HOXD1 function. We show that haploinsufficiency at this locus results in the splitting of horn bud primordia, likely following the abnormal extension of an initial morphogenetic field. These results highlight the key role played by this gene in headgear patterning and illustrate the evolutionary co-option of a gene involved in the early development of bilateria to properly fix the position and number of these distinctive organs of Bovidae.

Published
2021
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67. Lamb meat traceability: The case of Sambucana sheep

Author

Alfredo Pauciullo, Liliana Di Stasio, Emiliano Lasagna, Daniele Bigi, Piergiovanni Piatti, Edoardo Fontanella, Stefano Costa, Di Stasio, Liliana, Piatti, Piergiovanni, Fontanella, Edoardo, Testa, Stefano, Bigi, Daniele, Lasagna, Emiliano, and Pauciullo, Alfredo

Subjects
0106 biological sciences, Meat, Sheep, Traceability, Computer science, business.industry, Genetic traceability, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Certification, Registered trademark, 040201 dairy & animal science, 010603 evolutionary biology, 01 natural sciences, Biotechnology, Product certification, Food Animals, Genetic traceability, Meat, Sheep, Sambucana breed, Animal Science and Zoology, Sambucana breed, business
Abstract

Genetic traceability has a key role in the product certification, but it is rarely implemented in sheep so far, especially in the fresh meat sector. In this study, the case of the Sambucana sheep is analysed with the aim of developing a genetic system able to certify the origin of its traditional product, the Sambucano lamb, protected by a registered trademark. A set of 14 microsatellite markers was identified as an efficient tool to genetically discriminate the Sambucana sheep from other breeds potentially involved in mislabelling and to allow for an effective allocation test of meat cuts labelled as ‘Guaranteed Sambucano lamb’. The paternity test proved to be an additional means to improve the reliability of the control. The traceability system here described is easy to implement in local minor sheep breeds and is recommended in the framework of meat certification.

Published
2017

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