Search

Mitochondrial dysfunction is proposed to trigger memory deficits and synaptic damage at the onset of Alzheimer's disease (AD). However, it is unknown how mitochondria dysfunction might trigger synaptotoxicity and if a differential susceptibility of mitochondria located in synapses underlies the greater glutamatergic than GABAergic synaptotoxicity in early AD. Hippocampal synaptosomes (purified synapses) of a rat model of early AD, typified by selective memory deficits two weeks after intracerebroventricular injection of amyloid-β peptides (Aβ1-42, 2 nmol), simultaneously displayed three mitochondria-associated deleterious alterations: 1) hampered metabolism (decreased MTT reduction); 2) increased oxygen radical production (increased hydrogen peroxide production); 3) increased caspase-3 activity. The direct exposure of hippocampal synaptosomes to Aβ1-42 (500 nM) similarly decreased mitochondrial membrane potential (TMRM+ fluorescence) and increased mitochondria-derived oxygen radicals (MitoTraker®red-CM-H2Xros fluorescence) in individual glutamatergic (vesicular glutamate transporter-immunopositive) and GABAergic (vesicular GABA transporter-immunopositive) synaptosomes. However, significantly more glutamatergic than GABAergic synaptosomes were endowed with mitochondria (Tom20-immunopositive). These results indicate that dysfunctional mitochondria located in synapses can trigger synaptotoxicity through multifaceted mechanisms and that it is not the susceptibility of mitochondria to Aβ but more likely a different impact of dysfunctional mitochondria that underlies the greater sensitivity to synaptotoxicity of glutamatergic than GABA synapses in early AD.

The use of Complementary and Integrative Practices (CIP) is on the increase and its institutionalization in Primary Health Care (PHC) is a challenge. This article discusses the use, care, and policies of CIP at international and national levels found in the indexed literature. A review of the literature in PubMed/Medline and the Virtual Health Library was conducted using the key search words "Homeopathy", "Acupuncture", "Herbal Medicine", "Body Practices", "Primary Health Care" and other related terms in English, Spanish and Portuguese between 2002 and 2011. The use in the literature of CIP for the treatment of specific diseases from a biomedical perspective was observed, as well as evaluations of its use for the treatment of specific diseases focused on the reaction of the users and professionals and the analysis of the political, economic and social viability of CIP in health services. The conclusion drawn is that what is predominant in the literature is the quest for the scientific validation of CIP and a biomedical methodological bias in the designs of the studies, which does not contribute to clarifying the potential care of CIP in PHC.

Diatoms are widely used as bioindicators for the assessment of water quality in rivers and streams. Classically, the diatom biotic indices are based on the relative abundance of morphologically identified species weighted by their autoecological value. Obtaining such indices is time-consuming, costly, and requires excellent taxonomic expertise, which is not always available. Here we tested the possibility to overcome these limitations using a next-generation sequencing (NGS) approach to identify and quantify diatoms found in environmental DNA and RNA samples. We analyzed 27 river sites in the Geneva area (Switzerland), in order to compare the values of the Swiss Diatom Index (DI-CH) computed either by microscopic quantification of diatom species or directly from NGS data. Despite gaps in the reference database and variations in relative abundance of analyzed species, the diatom index shows a significant correlation between morphological and molecular data indicating similar biological quality status for the majority of sites. This proof-of-concept study demonstrates the potential of the NGS approach for identification and quantification of diatoms in environmental samples, opening new avenues toward the routine application of genetic tools for bioassessment and biomonitoring of aquatic ecosystems.

In this study, we analyzed the genetic diversity and structure of remnants of mangaba populations in states of northeastern Brazil by applying 9 microsatellite markers previously developed to establish conservation strategies for germplasm and species preservation. Six to 20 individuals per population were analyzed, with a total of 94 individuals and 6 populations from the states of Ceará, Pernambuco, and Sergipe, Brazil. The intra-population positive fixation index (f) in all populations indicated inbreeding resulting from the lack of random mating. The mean genetic diversity index values GST, FST, and RST estimated for divergence among the 6 populations were 0.14 (P < 0.05), revealing moderate genetic differentiation. The smallest FST value (P ≥ 0.05) was observed between the Jacarecoara and Tapera populations (0.005) and the highest between the Barra dos Coqueiros and Jacarecoara populations (0.287). The Jacarecoara population was the most divergent among the populations analyzed. According to analysis of molecular variance results, the largest variation percentage resulted from variability within populations (83.18%). Bayesian clustering analysis showed the formation of 2 sets (K = 2). Our results are important for developing strategies for in situ conservation of the species, seed collection, and ex situ conservation. For both methods, conservation of the greatest possible genetic variability of the species is essential.

Mitochondria are key players in the maintenance of cellular homeostasis, as they generate ATP via OXPHOS. As such, disruption in mitochondrial homeostasis is closely associated with disease states, caused by subtle alterations in the function of tissues or by major defects, particularly evident in tissues with high metabolic demands. Adaptations in mitochondrial copy number or mitochondrial mass, and the induction of genes implicated in OXPHOS or in intermediary metabolism as well, depend on the balanced contribution of both the nuclear and mitochondrial genomes. This forms a biogenesis program, controlled by several nuclear factors that act coordinately and in a categorized manner. Dynamic changes in mitochondrial regulators are associated with post-translational modifications mediated by metabolic sensors, such as SIRT1 and AMPK. Nrf2, which induces an antioxidant protective response against oxidative stress, also modulates bioenergetic function and metabolism. Additionally, the stability of mitochondrial transcripts is decreased by miRNA detected in the mitochondria, thus affecting the bioenergetic capacity of the cell. However, mitochondrial adaptation to metabolic demands is also dependent on the removal of damaged mitochondria (mitophagy) and fission/fusion events of the mitochondrial network.

The development of methods that enable analysis of programs and public policies is a key point for their objectives to be attained. This study presents a Brazilian experience of analysis of two food and nutritional security programs in cities in Brazil's northeast region, namely the National Program for Vitamin A Supplementation, and monitoring health conditions of those included in the Family Allowance Program. The methodological stages included the definition of programs to be analyzed, data collection locations, the population participating in the research, collection procedures and data analysis. The methodology used made it possible to ascertain the perception of the different social actors involved in the operationalization of these programs, proving to be an important tool for the insertion of these actors, not only as objects of analysis, but also mainly as subjects of self-evaluation.

Objective: This is an analytic, interventional, cross sectional study to evaluate the risk factors of post-dural (post-lumbar) puncture headache (PDPH) and the validity of the diagnostic criteria for PDPH from the ICHD II., Methods: Six-hundred-and-forty patients (332 non-pregnant women and 308 men) aged 8-65 years underwent spinal anesthesia with Quincke 25G or 27G needles in elective surgery., Results: Forty-eight (7.5%) of the patients developed PDPH. The binary logistic regression analysis identified as risk factors: gender [11.1% female vs. 3.6% male, OR 2.25 (1.07-4.73); p = 0.03], age [11.0% 31-50 years of age vs. 4.2% others, OR 2.21 (1.12-4.36); p = 0.02], previous history of PDPH [26.4% positive vs. 6.2% negative, OR 4.30 (1.99-9.31); p < 0.01] and bevel orientation [16.1% perpendicular vs. 5.7% parallel, OR 2.16 (1.07-4.35); p = 0.03]. The period of latency between lumbar puncture and headache onset range from 6 to 72 hours and the duration from 3 to 15 days. In 34/48 (71%) patients with PDPH, at least one of the following was present: neck stiffness, tinnitus, hypoacusia, photophobia, or nausea., Conclusion: In conclusion, 14/48 patients (29%) suffered none of the above-mentioned symptoms, indicating that a significant number of patients may suffer from PDPH in the absence of any symptoms apart from the headache itself. This suggests that a further analyses of existing studies should be made to determine if a criteria change may need consideration.

Background and Objectives: Intracranial subdural hematoma is a rare complication of spinal anesthesia. We report two cases of women who developed chronic subdural hematoma post-spinal anesthesia diagnosed after prolonged clinical evolution of post-dural puncture headache (PDPH) and we analyze other 33 cases found on literature review., Case Reports: In 35 patients (ages 20-88 years, 19 males), 14 were older than 60 years (40%), of which 12 (86%) were males. The relationship is inverted in the group of younger patients (< 60 years), in which we observed twice as many women (14:7). Two peaks of higher incidence were observed: 30-39 years (31%) and 60-69 years (29%). The length of time from the beginning of symptoms until diagnosis ranged from 4 hours to 29 weeks. Headache was the main complaint in 26/35 (74.3%) patients; changes in the level of consciousness in 14/35 (40.0%); vomiting in 11/35 (31,4%); hemiplegia or hemiparesis in 8/35 (22.9%); diplopia or VI nerve paresis in 5/35 (14.3%); and language disorders in 4/35 (11.4%). Contributing factors included: pregnancy, multiple punctures, use of anticoagulants, intracranial vascular abnormalities, and brain atrophy. In 15 cases, a contributing factor was not mentioned. Four out of 35 patients (11.4%) remained with neurologic sequelae, and 4/35 (11.4%) died., Conclusions: The presence of any of the signs or symptoms mentioned above should alert for the possibility of an intracranial subdural hematoma as a complication of puncture of the dura mater, especially in those patients with PDPH for more than one week, at which time investigation by neuroimaging is necessary., (© 2010 Elsevier Editora Ltda. All rights reserved.)

This study aimed to evaluate the National Program for Vitamin A Supplementation in a municipality (county) in Northeast Brazil, with participation by the parents or guardians of children under five years of age. This was a cross-sectional, population-based study using both qualitative and quantitative approaches. A total of 657 interviews were performed, and the vitamin A doses recorded on the children's immunization cards were analyzed. The population has access to high doses of vitamin A. However, knowledge of the vitamin A supplementation program was limited, especially among parents with less schooling. The program's coverage fell short of the Ministry of Health's target, especially after 18 months of age. It is thus necessary to intensify the program's educational activities in order to raise awareness among families of under-five children on the importance of vitamin A, its supplementation, and the importance of eating natural sources of this nutrient for the child's health.

Background and Objectives: The practice of Anesthesiology is exhausting and stressful. The present study evaluated the quality of life of anesthesiologists working in the city of Recife, correlating the profile of the quality of life with the degree of satisfaction with their health, number of working days and weekly shifts, gender, and age group., Methods: The WHOQOL-BREF questionnaire was used. Spearman's correlation and Student t test, ANOVA, and Scheffé test for variance were used, considering a p < 0.05 significant., Results: Subjective analysis demonstrated that 44.6% of anesthesiologists have a negative or undefined perception about their quality of life. The degree of satisfaction with their health (r = 0.525; p = 0.01) and the excess of weekly working shifts (p = 0.03) had a significant influence on the results. Female anesthesiologists had significantly lower scores than males in the subjective evaluation of quality of life and in the psychological and social relationships domains. A significant difference was not observed among different age groups. The environmental domain had lower scores than the others in all parameters analyzed., Conclusions: Long working hours represented a negative factor in quality of life of all anesthesiologists in the city of Recife, and that of female anesthesiologists was significantly worse than males. Therefore, we concluded that reflection and actions are required to influence positively the health and quality of life of those professionals. Besides individual actions, institutional support is fundamentally important for the practice of anesthesiology and, to achieve positive changes, decisions should be based on scientific evidence.

The aim of this study was to determine if patients with a previous history of postdural puncture headache (PDPH) might be prone to a new episode after spinal anaesthesia. Consecutive patients (n = 258) who had had surgery under spinal anaesthesia were studied. Of 42 patients with a previous history of PDPH, eight (19.0%) developed a new PDPH episode; whereas out of 216 without a previous history of PDPH, only 15 (6.9%) presented with PDPH. Previous PDPH history indicates a higher chance of a new episode of PDPH after spinal anaesthesia. Women are more susceptible to such recurrences.

The single and combined effects of three healthy lifestyle behaviors-nonsmoking, being physically active, and having a high-quality diet-on survival were investigated among older people in the SENECA Study. This European longitudinal study started with baseline measurements in 1988-1989 and lasted until April 30, 1999. The study population consisted of 631 men and 650 women aged 70-75 years from Belgium, Denmark, Italy, The Netherlands, Portugal, Spain, and Switzerland. A lifestyle score was calculated by adding the scores of the lifestyle factors physical activity, dietary quality, and smoking habits. The single lifestyle factors and the lifestyle score were related to mortality. Even at ages 70-75 years, the unhealthy lifestyle behaviors smoking, having a low-quality diet, and being physically inactive were singly related to an increased mortality risk (hazard ratios ranged from 1.2 to 2.1). The risk of death was further increased for all combinations of two unhealthy lifestyle behaviors. Finally, men and women with all three unhealthy lifestyle behaviors had a three- to fourfold increase in mortality risk. These results underscore the importance of a healthy lifestyle, including multiple lifestyle factors, and the maintenance of it with advancing age.

In 19 towns and cities across Europe anthropometric data--body weight, height, skinfold thicknesses and circumferences--have been obtained from 2332 elderly subjects born between 1913 and 1918, according to a strictly standardized methodology. Large variations exist among research towns even within countries. For body weight, means ranged from 70.1 +/- 15.4 kg to 78.2 +/- 10.7 kg in men and from 56.8 +/- 8.1 to 71.4 +/- 11.4 kg in women. In the North European towns and cities both men and women were taller than their counterparts in the southern towns. Mean body mass index varied from 24.4 +/- 3.8 kg m-2 to 30.3 +/- 5.2 kg m-2 among men. In women the range of means was from 23.9 +/- 3.6 kg m-2 to 30.5 +/- 5.1 kg m-2. Triceps skinfold thicknesses suggest that females had more fat at subcutaneous sites than the males, and the waist-hip ratio was consistently lower in elderly women.