58 results on '"Alloni, Rossana"'
Search Results
52. Effect of surgical stress on nuclear and mitochondrial DNA from healthy sections of colon and rectum of patients with colorectal cancer
- Author
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Umberto Mancini, Lucia Potenza, Emanuela Polidori, Roberta De Bellis, Cinzia Calcabrini, Sabrina Zeppa, Marina Dachà, Luigi Cucchiarini, Rossana Alloni, Potenza, Lucia, Calcabrini, Cinzia, De Bellis, Roberta, Mancini, Umberto, Polidori, Emanuela, Zeppa, Sabrina, Alloni, Rossana, Cucchiarini, Luigi, and Dachà, Marina
- Subjects
Male ,Colorectal cancer ,Gene Dosage ,DNA Glycosylases ,chemistry.chemical_compound ,Chromosomes, Human ,Cells, Cultured ,Digestive System Surgical Procedures ,Aged, 80 and over ,reactive oxygen species ,chemistry.chemical_classification ,Genetics ,surgical stress ,reactive oxygen specie ,Deoxyadenosines ,General Medicine ,Middle Aged ,Mitochondrial DNA, nuclear DNA, 8OH-dG, oxidative DNA damage, reactive oxygen species, surgical stress ,Mitochondrial DNA ,Nuclear DNA ,medicine.anatomical_structure ,Female ,surgical stre ,Colorectal Neoplasms ,General Agricultural and Biological Sciences ,Surgical stress ,Colon ,Rectum ,8OH-dG ,Biology ,DNA, Mitochondrial ,General Biochemistry, Genetics and Molecular Biology ,oxidative DNA damage ,Tissue damage ,medicine ,Humans ,Aged ,Reactive oxygen species ,Biochemistry, Genetics and Molecular Biology (all) ,nuclear DNA ,NADH Dehydrogenase ,medicine.disease ,Actins ,Oxidative Stress ,chemistry ,Agricultural and Biological Sciences (all) ,Cancer research ,DNA ,DNA Damage - Abstract
Surgical resection at any location in the body leads to stress response with cellular and subcellular change, leading to tissue damage. The intestine is extremely sensitive to surgical stress with consequent postoperative complications. It has been suggested that the increase of reactive oxygen species as subcellular changes plays an important role in this process. This article focuses on the effect of surgical stress on nuclear and mitochondrial DNA from healthy sections of colon and rectum of patients with colorectal cancer. Mitochondrial DNA copy number, mitochondrial common deletion and nuclear and mitochondrial 8-oxo-2′-deoxyguanosine content were measured. Both the colon and rectal tissue were significantly damaged either at the nuclear or mitochondrial level. In particular, mitochondrial DNA was more damaged in rectum than in colon. The present investigation found an association between surgical stress and nuclear and mitochondrial DNA damage, suggesting that surgery may generate an increase in free radicals, which trigger a cascade of molecular changes, including alterations in DNA. © 2011 Indian Academy of Sciences.
- Published
- 2011
53. Effects of reactive oxygen species on mitochondrial content and integrity of human anastomotic colorectal dehiscence: A preliminary DNA study
- Author
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Roberto Coppola, Roberta De Bellis, Cinzia Calcabrini, Umberto Mancini, Luigi Cucchiarini, Lucia Potenza, Marina Dachà, Gennaro Nappo, Laura Dugo, Rossana Alloni, Michele Guescini, Potenza, Lucia, Calcabrini, Cinzia, De Bellis, Roberta, Guescini, Michele, Mancini, Umberto, Cucchiarini, Luigi, Nappo, Gennaro, Alloni, Rossana, Coppola, Roberto, Dugo, Laura, and Dachà, Marina
- Subjects
Male ,medicine.medical_specialty ,Colon ,medicine.medical_treatment ,Rectum ,Anastomotic dehiscence ,Anastomosis ,Dehiscence ,medicine.disease_cause ,DNA, Mitochondrial ,Polymerase Chain Reaction ,Surgical Wound Dehiscence ,medicine ,Humans ,lcsh:RC799-869 ,Neoadjuvant therapy ,Aged ,chemistry.chemical_classification ,Aged, 80 and over ,Reactive oxygen species ,business.industry ,Gastroenterology ,General Medicine ,Middle Aged ,Mitochondrial DNA ,Colorectal surgery ,Neoadjuvant Therapy ,Surgery ,Oxidative Stress ,medicine.anatomical_structure ,chemistry ,Oxidative DNA damage ,Reactive oxygen specie ,lcsh:Diseases of the digestive system. Gastroenterology ,Original Article ,Female ,business ,Colorectal Neoplasms ,Reactive Oxygen Species ,Oxidative stress - Abstract
BACKGROUND: Anastomotic dehiscence is one of the most severe complications of colorectal surgery. Gaining insight into the molecular mechanisms responsible for the development of anastomotic dehiscence following colorectal surgery is important for the reduction of postoperative complications.OBJECTIVE: Based on the close relationship between surgical stress and oxidative stress, the present study aimed to determine whether a correlation exists between increased levels of reactive oxygen species and colorectal anastomotic dehiscence.METHODS: Patients who underwent surgical resection for colorectal cancer were divided into three groups: patients with anastomotic dehiscence (group 1); patients without dehiscence who underwent neoadjuvant radiochemotherapy (group 2); and patients without anastomotic dehiscence who did not undergo neoadjuvant radiochemotherapy (group 3). Quantitative polymerase chain reaction and real-time polymerase chain reaction assays were performed to measure nuclear DNA and mitochondrial DNA (mtDNA) content, and possible oxidative damage to nonmalignant colon and rectal tissues adjacent to the anastomoses.RESULTS: mtDNA content was reduced in the colon tissue of patients in groups 1 and 2. Rectal mtDNA was found to be more damaged than colonic mtDNAs in all groups. The 4977 bp common deletion was observed in the mtDNA of tissues from both the colon and rectum of all patients.DISCUSSION: Patients in groups 1 and 2 were more similar to one another than to group 3, probably due to higher levels of reactive oxygen species in the mitochondria; the greater damage found in the rectum suggests that dehiscence originates primarily from the rectal area.CONCLUSIONS: The present study of mtDNA analyses of normal human colon and rectal tissues from patients with colorectal cancer is among the first of its kind.
54. Compliance with the Surgical Safety Checklist Results of an Audit in a Teaching Hospital in Italy.
- Author
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Alloni R, De Benedictis A, Nobile L, Sica L, Pensieri C, Sechi MR, and D'Aprile M
- Subjects
- Attitude of Health Personnel, Humans, Italy, Nurses statistics & numerical data, Operating Room Nursing standards, Personnel Delegation, Quality Improvement, Surgeons statistics & numerical data, Surveys and Questionnaires, Checklist, Guideline Adherence, Hospitals, University, Nurses psychology, Practice Patterns, Nurses' statistics & numerical data, Practice Patterns, Physicians' statistics & numerical data, Safety Management standards, Surgeons psychology
- Abstract
Aim: We carried out an audit to verify compliance to Surgical Safety Checklist (SSC), as we have become aware that compliance across different teams and by individual surgeons has not been optimal., Material of Study: 100 SSC records from October-December 2014 and 100 from March-June 2015 were inspected to verify correct . 44 surgeons and 34 scrub nurses were asked to complete a questionnaire to know surgeons' compliance to the different stages of the Checklist and the compliance of each surgical team. 100% of scrub nurse and 73.7% of surgeons completed the questionnaire., Results: All Checklist records were correctly filled out but we could verify that while nurses have a strong commitment to the SSC, the Checklist's implementation is not being actively supported by all surgical team members., Discussion: Many surgeons showed limited awareness of not collaborating during SSC procedure and admitted delegating the responsibility for answering questions to other members of their team. A number of them fell into contradiction answering to various parts of the questionnaire. Consistent with the literature, at our hospital there is a gap between quality of Checklist paper records and correct use of this safety tool., Conclusions: Thanks to the data we have collected we will improve the way the SSC is used and promote change in the behavior of surgeons. Eighteen surgeons (40.9%) expressed willingness to be involved in a work group to revise the SSC and we hope that their commitment to safety and quality will increase., Key Words: Surgical Safety Checklist, Surgeons commitment.
- Published
- 2016
55. Melena as presentation of primary small intestine inflammatory myofibroblastic tumor in an adult woman. A case report.
- Author
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Alloni R, Ancona G, Gallo I, Crescenzi A, and Coppola R
- Subjects
- Adult, Diagnosis, Differential, Female, Gastrointestinal Stromal Tumors diagnosis, Granuloma, Plasma Cell complications, Granuloma, Plasma Cell pathology, Granuloma, Plasma Cell surgery, Humans, Intussusception etiology, Jejunal Diseases complications, Jejunal Diseases pathology, Jejunal Diseases surgery, Granuloma, Plasma Cell diagnosis, Jejunal Diseases diagnosis, Melena etiology
- Abstract
Introduction: Inflammatory myofibroblastic tumor (IMT), also known as inflammatory pseudotumor, plasma cell granuloma or inflammatory myofibroblastoma, is characterized histopathologically by myofibroblastic spindle cells with inflammatory cell infiltrates ( plasma cell, lymphocytes and eosinophils). Inflammatory myofibroblastic tumor is tipically seen in children or young adults and is most commonly localized in the lung, but it can occur anywhere in the body., Case Report: We present a case in a young woman with severe acute G.I. bleeding, an uncommon presentation of IMT in adults. The patient was admitted to the emergency department for melena. MRI showed a distal jejunum hypervascular mass. Other exams were negative. Surgical excision was recommended, so the patient underwent surgery with complete removal of the tumoral mass. No adjuvant therapy was employed and the patient is asymptomatic after 6 months of follow-up., Discussion: IMT is an rare lesion that mimics malignancy and is accompanied by various clinical manifestations. The treatment of choice is believed to be complete surgical excision and long term follow up., Key Words: Anemia, Inflammatory myofibroblastic tumor (IMT), Small intestine, Surgery.
- Published
- 2015
56. Effects of oxidative stress on mitochondrial content and integrity of human anastomotic colorectal dehiscence: a preliminary DNA study.
- Author
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Potenza L, Calcabrini C, De Bellis R, Guescini M, Mancini U, Cucchiarini L, Nappo G, Alloni R, Coppola R, Dugo L, and Dacha M
- Subjects
- Aged, Aged, 80 and over, Colon physiopathology, Colorectal Neoplasms therapy, Female, Humans, Male, Middle Aged, Neoadjuvant Therapy methods, Polymerase Chain Reaction methods, Reactive Oxygen Species metabolism, Rectum physiopathology, Surgical Wound Dehiscence etiology, Colorectal Neoplasms surgery, DNA, Mitochondrial metabolism, Oxidative Stress, Surgical Wound Dehiscence pathology
- Abstract
Background: Anastomotic dehiscence is one of the most severe complications of colorectal surgery. Gaining insight into the molecular mechanisms responsible for the development of anastomotic dehiscence following colorectal surgery is important for the reduction of postoperative complications., Objective: Based on the close relationship between surgical stress and oxidative stress, the present study aimed to determine whether a correlation exists between increased levels of reactive oxygen species and colorectal anastomotic dehiscence., Methods: Patients who underwent surgical resection for colorectal cancer were divided into three groups: patients with anastomotic dehiscence (group 1); patients without dehiscence who underwent neoadjuvant radiochemotherapy (group 2); and patients without anastomotic dehiscence who did not undergo neoadjuvant radiochemotherapy (group 3). Quantitative polymerase chain reaction and real-time polymerase chain reaction assays were performed to measure nuclear DNA and mitochondrial DNA (mtDNA) content, and possible oxidative damage to nonmalignant colon and rectal tissues adjacent to the anastomoses., Results: mtDNA content was reduced in the colon tissue of patients in groups 1 and 2. Rectal mtDNA was found to be more damaged than colonic mtDNAs in all groups. The 4977 bp common deletion was observed in the mtDNA of tissues from both the colon and rectum of all patients., Discussion: Patients in groups 1 and 2 were more similar to one another than to group 3, probably due to higher levels of reactive oxygen species in the mitochondria; the greater damage found in the rectum suggests that dehiscence originates primarily from the rectal area., Conclusions: The present study of mtDNA analyses of normal human colon and rectal tissues from patients with colorectal cancer is among the first of its kind.
- Published
- 2011
- Full Text
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57. Gallstone ileus: a rare cause of colon obstruction. Report of a case.
- Author
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Valeri S, Garberini A, Alloni R, and Coppola R
- Subjects
- Aged, 80 and over, Colonic Diseases diagnosis, Colonic Diseases surgery, Female, Humans, Intestinal Obstruction diagnosis, Intestinal Obstruction surgery, Colonic Diseases etiology, Gallstones complications, Intestinal Obstruction etiology
- Abstract
The colon is a rare site of gallstone ileus. We report the case of a woman with cholelithiasis who underwent a total colectomy and cholecystectomy for colon obstruction due to a gallstone impacted in the sigmoid colon narrowed by diverticulitis.
- Published
- 2008
58. [Education in postgraduate surgical schools: the role of the surgical tutor as supervisor in the operating room].
- Author
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Alloni R, Binetti P, Coppola R, and Arullani A
- Subjects
- Guidelines as Topic, Humans, Italy, Physician's Role, Surveys and Questionnaires, Teaching, Faculty, Medical organization & administration, General Surgery education, Internship and Residency organization & administration, Mentors, Operating Rooms organization & administration, Schools, Medical organization & administration
- Abstract
The Postgraduate Surgical education is in an era of transition, in order to create physicians with skills and attitudes needed by modern health care. Many studies have examined the impact of surgical tutoring in surgical residency programs in USA Medical Schools, while few experiences are reported from European Universities. The new Italian guidelines for post-graduate education require a structured clinical learning with the supervision of a tutor ("attending surgeon" for surgical residency); it is a challenge to describe the role of this teacher and educator, and to implement an effective evaluation of operating room teachers. Confidential survey was administered to 14 surgical residents of the Authors' University. Questions were related to their surgical activity and their perception of educational role of tutors in operating room and tutors' teaching behaviors. Residents pointed out five behaviors they perceive as signs of tutor excellence in clinical and operating room setting. According with studies from other Universities, residents need a tutor with competency but also with good teaching skills and a mature self-perception as educator. Faculty would provide training programs for surgeons in order to improve their teaching skills and behaviors.
- Published
- 2005
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