51. Granulin: An Invasive and Survival-Determining Marker in Colorectal Cancer Patients
- Author
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Clemens Franz, Christoph Kahlert, Alexis Ulrich, Fee Klupp, Naita Maren Wirsik, Nikolai Schleussner, Niels Halama, Arne Warth, and Thomas Schmidt
- Subjects
Male ,Angiogenesis ,Colorectal cancer ,QH301-705.5 ,granulin ,Gene Expression ,Granulin ,colorectal cancer ,survival ,Article ,Catalysis ,Inorganic Chemistry ,Downregulation and upregulation ,Biomarkers, Tumor ,Humans ,Medicine ,Neoplasm Invasiveness ,Physical and Theoretical Chemistry ,Biology (General) ,Molecular Biology ,QD1-999 ,Spectroscopy ,Aged ,Granulins ,Neoplasm Staging ,Aged, 80 and over ,business.industry ,Organic Chemistry ,adenomas ,General Medicine ,Transfection ,Middle Aged ,HCT116 Cells ,Prognosis ,medicine.disease ,Immunohistochemistry ,Survival Analysis ,In vitro ,Computer Science Applications ,Chemistry ,Over expression ,Cancer research ,Female ,Colorectal Neoplasms ,business ,GRN - Abstract
Background: Granulin is a secreted, glycosylated peptide—originated by cleavage from a precursor protein—which is involved in cell growth, tumor invasion and angiogenesis. However, the specific prognostic impact of granulin in human colorectal cancer has only been studied to a limited extent. Thus, we wanted to assess the expression of granulin in colorectal cancer patients to evaluate its potential as a prognostic biomarker. Methods: Expressional differences of granulin in colorectal carcinoma tissue (n = 94) and corresponding healthy colon mucosa were assessed using qRT-PCR. Immunohistochemistry was performed in colorectal cancer specimens (n = 97), corresponding healthy mucosa (n = 47) and colorectal adenomas (n = 19). Subsequently, the results were correlated with histopathological and clinical patients’ data. HCT-116 cells were transfected with siRNA for invasion and migration assays. Results: Immunohistochemistry and qRT-PCR revealed tumoral over expression of granulin in colorectal cancer specimens compared to corresponding healthy colon mucosa and adenomas. Tumoral overexpression of granulin was associated with a significantly impaired overall survival. Moreover, downregulation of granulin by siRNA significantly diminished the invasive capacities of HCT-116 cells in vitro. Conclusion: Expression of granulin differs in colorectal cancer tissue, adenomas and healthy colon mucosa. Furthermore, granulin features invasive and migrative capabilities and overexpression of granulin correlates with a dismal prognosis. This reveals its potential as a prognostic biomarker and granulin could be a worthwhile molecular target for individualized anticancer therapy.
- Published
- 2021
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