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381 results on '"Abbate, I."'

54. Changes in host cell molecules acquired by circulating HIV-1 in patients treated with highly active antiretroviral therapy and interleukin-2.

Author

Abbate, I, Dianzani, F, Turriziani, O, Antonelli, G, D'Offizi, G, Galati, V, Pierdominici, M, Pandolfi, Franco, Capobianchi, Mr, Pandolfi, Franco (ORCID:0000-0001-8799-8173), Abbate, I, Dianzani, F, Turriziani, O, Antonelli, G, D'Offizi, G, Galati, V, Pierdominici, M, Pandolfi, Franco, Capobianchi, Mr, and Pandolfi, Franco (ORCID:0000-0001-8799-8173)

Published
2001

90. Evaluation of an automated extraction system in combination with Affigene® CMV Trender for CMV DNA quantitative determination: Comparison with nested PCR and pp65 antigen test

Author

Abbate, I., Finnstrom, N., Zaniratti, S., Solmone, M.C., Selvaggini, S., Bennici, E., Neri, S., Brega, C., Paterno, M., and Capobianchi, M.R.

Subjects
*BIOMOLECULES, *CYTOMEGALOVIRUS diseases, *HERPESVIRUS diseases, *MOLECULAR biology
Abstract

Abstract: CMV viral load quantitation is a powerful tool to assist clinicians in making accurate diagnoses, managing post-transplant CMV disease and monitoring antiviral therapy. The aim of this study was to evaluate the performance of Affigene® CMV Trender for CMV viral load determination used in combination with a non-dedicated nucleic acid extraction system (BioRobot MDx) for high-throughput routine. Linearity, reproducibility and sensitivity were examined. Clinical samples were used to compare results obtained with the Affigene® CMV Trender, with an “in house” nested PCR used for routine diagnosis and with pp65 antigenemia. The results indicated that the test is linear in the range of 1.81–5.18Logcopies/ml and that sensitivity is 77copies/ml. The concordance of the Affigene® CMV Trender with nested PCR was high, (k =0.91, IC 95%=0.82–1.00), whereas a substantial concordance with pp65 antigenemia was observed (k =0.64, IC 95%=0.54–0.73). In conclusion, combined use of a non-dedicated automated nucleic acid extraction method with the Affigene® CMV Trender results in an accurate high throughput system, suitable for routine laboratory monitoring of CMV infection. [Copyright &y& Elsevier]

Published
2008
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91. Expression of Werner and Bloom syndrome genes is differentially regulated byin vitroHIV-1 infection of peripheral blood mononuclear cells.

Author

BORDI, L., AMENDOLA, A., CICCOSANTI, F., ABBATE, I., CAMILLONI, G., and CAPOBIANCHI, M. R.

Subjects
HIV infections, AGING, MESSENGER RNA, LENTIVIRUS diseases, DNA polymerases, POLYMERASE chain reaction
Abstract

In HIV infection, continuous immune activation leads to accelerated ageing of the adaptive immune system, similar to that observed in elderly people. We investigated the expression of WRN and BLM (genes involved in disorders characterized by premature ageing, genomic instability and cancer predisposition) in peripheral blood mononuclear cells (PBMC) activatedin vitrowith phytohaemagglutinin (PHA) and infected with different HIV-1 strains. The steady state levels of mRNA were analysed by reverse transcription-polymerase chain reaction (RT-PCR), and protein expression was assayed using immunocytochemistry and Western blot techniques. In uninfected PBMC, PHA stimulation induced an increase in BLM mRNA and protein expression, while WRN expression remained virtually unchanged. When PBMC were infectedin vitrowith a lymphotropic HIV-1 strain, the level of BLM mRNA showed a peak at 24 h of infection, followed by a decline to uninfected culture levels. A similar result failed to be seen using an R5-tropic HIV-1 strain. In accordance with mRNA expression, in HIV-infected cultures PBMC were stained more frequently and more intensely by a BLM-specific antibody as compared to uninfected cultures, staining peaking at 24. Conversely, WRN expression was not modulated by HIV-1. The proportion of cells showing BLM up-regulation, established by immunocytochemical staining, was much greater than the proportion of productively infected PBMC, as established by proviral DNA measurement. This result indicates that BLM up-regulation is probably a result of an indirect bystander cell effect. Activation of the BLM gene in infected PBMC suggests that premature ageing could be a further immunopathogenetic mechanism involved in HIV-induced immunodeficiency, and points to a possible new candidate target for innovative therapeutic intervention. [ABSTRACT FROM AUTHOR]

Published
2004
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93. Acute infection with parvovirus B19 manifesting as brain stem encephalitis [corrected] [published erratum appears in INFECT MED 2008 Jun;25(6):301].

Author

Moretti MV, Abbate I, Sciarrone MR, Guercini G, Francisci D, Pelliccioli GP, and Baldelli F

Abstract

Diseases associated with Parvovirus B19 infection are well known in pediatrics, obstetrics, and hematology, but the full clinical spectrum of these diseases has not yet been defined. We describe a patient with HIV infection and profound immunosuppression who subsequently developed brain stem encephalitis (rhombencephalitis) and transfusion-dependent anemia as a consequence of Parvovirus B19 infection. To our knowledge, this is the first reported case in which neurological manifestations coincided with evidence of acute infection with Parvovirus B19. [ABSTRACT FROM AUTHOR]

Published
2008

96. Heterogeneity of HVR-1 quasispecies is predictive of early but not sustained virological response in genotype 1b-infected patients undergoing combined treatment with PEG- or STD-IFN plus RBV

Author

Abbate, I., Cappiello, G., Lo Iacono, O., Longo, R., Ferraro, D., Antonucci, G., Di Marco, V., Di Stefano, R., Antonio Craxi, Solmone, M. C., Spanò, A., Ippolito, G., Capobianchi, M. R., Abbate, I., Cappiello, G., LO IACONO, O., Longo, R., Ferraro, D., Antonucci, G., DI MARCO, V., DI STEFANO, R., Craxi, A., Solmone, M., Spanò, A., Ippolito, G., and Capobianchi, M.

Subjects
Genotype, Hepacivirus, Interferon alpha-2, Viral Nonstructural Proteins, Antiviral Agents, Polyethylene Glycols, Viral Proteins, Genetic Heterogeneity, Ribavirin, Humans, Viral Protein, Phylogeny, Antiviral Agent, Hepaciviru, Viral Nonstructural Protein, Interferon-alpha, Sequence Analysis, DNA, Hepatitis C, Chronic, Recombinant Protein, Viral Load, Recombinant Proteins, Treatment Outcome, Linear Models, Linear Model, Drug Therapy, Combination, Sequence Alignment, Human
Abstract

ISDR mutation pattern and HVR-1 quasispecies were analyzed in HCV genotype 1b-infected patients treated with either PEG- or STD-IFN plus ribavirin, in order to find virological correlates of therapy outcome. ISDR region analysis, performed at baseline (T0) and at 4 weeks of therapy (T1), indicated that ISDR mutation pattern was not predictive of response to treatment. Moreover, no selection of putative resistant strains in the first month of therapy was observed. Viral load was not correlated with any parameter of HVR-1 heterogeneity. Among the HVR-1 heterogeneity parameters considered, complexity was inversely correlated to viral load decline at T1. In univariate analysis, complexity, proportion of non synonymous substitutions (NS) and NS/S ratio were lower in patients showing virological response at 6 months of treatment. Complexity was the only parameter independently associated with both decline of viral load at T1 and virological response after 6 months, even after adjustment for confounding variables. At the end of treatment or later, these correlations were lost. Evolution pattern of the HVR-1 quasispecies indicated a strong selective pressure in sustained responders, with complete substitution of pre-existing quasispecies, while minor changes occured in non responders. In relapsers both patterns were present at a similar rate. In conclusion, this study shows that HVR-1 heterogeneity may be involved in the early response to combined IFN-RBV therapy. The loss of correlation between viral heterogeneity and therapy outcome at 6 months of therapy, or later, suggests that other factors may play a role in maintaining sustained response to treatment.

97. Cervicovaginal shedding of TT virus in HIV-infected women

Author

Calcaterra, S., Zaniratti, M. S., Serraino, D., Peroni, M., Abbate, I., Cappiello, G., Pierluca Piselli, Pavia, C., Rezza, G., Ippolito, G., and Capobianchi, M. R.

Subjects
Adult, Torque teno virus, AIDS-Related Opportunistic Infections, Papillomavirus Infections, Cervix Uteri, Middle Aged, DNA Virus Infections, Virus Shedding, Tumor Virus Infections, Italy, DNA, Viral, Vagina, Prevalence, Humans, Female, Papillomaviridae
Abstract

TT virus (TTV) is frequently detected in the serum and in other body fluids of humans. Recently TTV-specific deoxyribonucleic acid has been detected in cervical specimens from apparently healthy women and in seminal fluid, suggesting that sexual transmission may be common.TT virus-deoxyribonucleic acid prevalence was assessed in paired samples of blood and cervical smears from 110 human immunodeficiency virus-positive women. Detection and typing of human papillomavirus (HPV) present in cervical smears was also performed.The prevalence of TTV-deoxyribonucleic acid in cervical smears was 16.4%, without significant difference (p = 0.81) between HPV-positive (18.6%) and -negative (14.9%) samples. The distribution of high/middle and low-risk HPV types was similar in TTV-positive and -negative samples. On the contrary, women with multiple HPV infections had a significantly higher TTV-deoxyribonucleic acid prevalence (60.0%) than HPV-negative women (p = 0.04).TT virus excretion in the female genital tract of human immunodeficiency virus-infected women is common, further supporting sexual transmission of this virus.

100. Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing

Author

Bartolini, B., Giovanni Chillemi, Abbate, I., Bruselles, A., Rozera, G., Castrignanò, T., Paoletti, D., Picardi, E., Desideri, A., Pesole, G., and Capobianchi, M. R.

Subjects
virus diseases, High-throughput pyrosequencing, Influenza, Nasopharyngeal swabs
Abstract

De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasopharyngeal swabs in the context of the microbial community. Data were generated with a prior sequenceindependent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full-length genome reconstruction with the simultaneous analysis of site-specific heterogeneity. The molecular approach applied proved to be a powerful tool to characterize the new pandemic H1N1 influenza virus in clinical samples. This approach could be of great value in identifying possibly new reassortants that may occur in the near future.

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