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Aim - improvement of efficiency assessment methods of therapy of mycoplasma infection in children with bronchial asthma. The effectiveness of treatment of mycoplasma infection in the period of exacerbation of bronchial asthma in 250 children, aged 1 to 7 years, was evaluated. The children were on basic therapy and received treatment with azithromycin: three courses at a dose of 10 mg/kg of weight for 3 days with an interval of 4 days 5-7 days. Microbiological (culturing), immunological (DIF, AHAA), and genetic (PCR) methods were used to identify mycoplasma markers. The main focus was on identifying two species - M. pneumoniae and M. hominis, most commonly found in mycoplasma respiratory infections, including bronchial asthma. In 250 children with bronchial asthma, antigens of Mycoplasma pneumoniae, Mycoplasma hominis, Ureaplasma urealyticum, Mycoplasma arthritidis and Mycoplasma fermentrans were detected in 62,8%, 42,8%, 46,8 %, 31,6%, 45,6% of cases, respectively. A detailed study of the presence of M. pneumoniae and M. hominis antigens in the blood of 83 children with bronchial asthma showed that before treatment, the detection rate of M. pneumoniae and M. hominis antigens was 67.5% and 50.6%, respectively, in the CIC - 65.1% and 61.5%, DNA in the blood serum - 4.8% and 16.9%, and in the CIC - 27.7% and 32.5%, respectively. From 7 CIC samples containing M. hominis DNA and 2 CIC samples containing M. pneumoniae DNA, atypical cultures of "mini-colonies" of M. hominis and M. pneumoniae were isolated, the specificity of which was confirmed not only by DIF and PCR, but also by the ability to grow on a solid medium for mycoplasmas. After treatment by azithromycin, the number of positive tests on antigens and DNA in free state and in structure of CIC significantly decreased. The identification of specific markers of mycoplasma cells in the comprehensive diagnostics of mycoplasma infection in children with exacerbation of asthma, increases the effectiveness of therapy control for mycoplasma infection and improves the prognosis of bronchial asthma in patients.

This paper reports a study of biofilm formation by the Mycoplasma pneumoniae (Mp) strain Fh on an abiotic surface using light and electron microscopy. Quantitative assessment of the effectiveness of biofilm formation was carried out in polystyrene plates with crystal-Violet cell staining. To study the ultrastructural organization of the Mp cells, scanning electron microscopy was used, which allowed a well-formed multicellular clearly defined structure of considerable size to be revealed by the third day of culturing in which Mp cells were submerged into the extracellular matrix with a clearly detectable system of pores. The morphology of the detected aggregations was absolutely identical to that of the biofilms described for other microorganisms, which confirmed that Mp had the ability to to form biofilms. The described phenomenon of the ability of Mp to grow in the form of the biofilm is of great importance both for understanding the causes of the long-term persistence of Mp in the human organism and for the development of new approaches for the treatment of protracted and chronic forms of infectious diseases caused by Mp.

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Mycoplasma hominis is an opportunistic bacterium that can cause acute and chronic infections of the urogenital tract. This bacterium, like all other Mycoplasma species, is characterized by the reduced genome size, and, consequently, reduction of the main metabolic pathways. M. hominis cells cannot effectively use glucose as a carbon and energy source. Therefore, the main pathway of energy metabolism is the arginine dihydrolase pathway. However, several bacteria can use nucleosides as the sole energy source. Biochemical studies using Salmonella typhimurium have shown that three enzymes (thymidine phosphorylase, phosphopentose mutase and deoxyribose-phosphate aldolase) are involved in the thymidine catabolic pathway. All these enzymes are present in M. hominis. For understanding changes in the energy metabolism of M. hominis we performed shotgun proteome analysis of M. hominis cells in liquid medium with arginine or thymidine as a carbon source. LC-MS analysis was performed with an Ultimate 3000 Nano LC System (Thermo Fisher Scientific) coupled to a Q Exactive HF benchtop Orbitrap mass spectrometer (Thermo Fisher Scientific) via a nanoelectrospray source (Thermo Fisher Scientific). Data are available via ProteomeXchange with identifier PXD018714 (https://www.ebi.ac.uk/pride/archive/projects/PXD018714).

Today much attention is paid to processing textual information in order to form thematic groups and to systematize documents. This is stipulated by growing popularity of the Internet as a means of communication and requires to categorize short technical texts, which, in turn, is characterized by complexity of traditional approaches - preprocessing and digitization of documents and identification of "classifying" features. Specificity of the study at each stage is determined by the characteristics of the texts - small size, similar vocabulary, a large number of highly specialized symbols and signs, synonymity of terms.There has been suggested the procedure of preparing texts for analysis, reducing the dimensions of "term-document" matrix using singular decomposition method which allows to solve the problem of small-rank approximation of the original matrix. There are classification methods used such as k-nearest neighbors method and discriminant analysis based on Fisher elementary functions (texts on assignment of instruments was taken as an example). The Fisher classification procedure uses discriminant variables and the approach of maximizing the differences between classes to obtain the classification function. An object belongs to the class for which the value of classifying function is the greatest. There has been given assessment of the results obtained and the inadequate accuracy of classification when applying TF-IDF measure under experimental conditions. To improve the quality of classification, a combined method has been proposed to select words at the first step using TF-IDF measure. The dictionary of terms and phrases is to be used at the second stage for classifying texts. According to the obtained data, it has been offered to carry out classification by discriminant analysis and k-closest neighbors method. The proposed combined method is planned to be refined and upgraded in the future.

Long-term cooperation FSBI “Gamaleya Federal Scientific Research Center of Epidemiology and Microbiology”, Russia and Research Institute of Medical Primatology, Russia on the research of spontaneous Mycoplasma infection in different monkey species of the Adler colony showed a wide prevalence of Mycoplasma and Ureaplasma carriers. Mycoplasma isolated from the apes and forming mini-colonies are similar to those isolated from patients with various chronic pathological processes. The cells of mini-colonies are resistant to various unfavourable factors causing the death of cells of classical colonies. The cells of mini-colonies are resistant to antibiotics, which have fundamentally different mechanisms of action. Key words: mycoplasma, ureaplasma, monkeys, mini-colonies, antibiotics.

The aim of this study was to assess the possibility of using wastes from pulp and paper industry as metal corrosion inhibitors. We studied protective properties of various systems, including the primers with rust modifiers developed on the basis of sulfite spent liquors and an upper coating layer of PF-115 enamel applied to a rusty surface. Using the gravimetric method and the method of polarization curves, we found that sulfite spent liquors inhibit steel corrosion in an acidic environment. We found that the P-2 rust modifier with an addition of lye + piperidine is the most effective inhibitor of steel rusting.

The article provides information on changes in the activity of centers of public health and medical prevention in conditions of CO-VID-19 pandemic Examples of response to the coronavirus outbreak of the world medical community are given, the developed strategies and the most effective restrictive measures are listed The result of a survey of 72 regions of the Russian Federation on changes in the work of public health and medical prevention centers that have occurred since the announcement of the pandemic and the establishment of the Coronavirus operation headquarters is presented The information about the development of new information materials, speeches in mass media, holding online events and live broadcasts for patients, providing medical and psy-chological assistance to the population at home and on the phone, organizing methodological assistance to medical organizations on the prevention of risk factors for chronic non-communicable diseases in COVID-19 conditions is listed Receiving new data on changes in the work of different spheres of health care system during the global pandemic will allow developing new algorithms of response in such situations and optimizing the functions of public health and medical prevention centers © 2020, Media Sphera Publishing Group All rights reserved

The clinical material obtained surgically in patients with kidney stone disease (KSD) was tested for content of the stone microflora using PCR and standard microbiological methods. It was demonstrated that about 50% of stones in patients with KSD were infected with various infection agents as observed using standard microbiological and molecular genetic methods. The percentage of detection of the Mycoplasma hominis using cultural method is lower than the percentage detected using PCR, which is due to difficult isolation and cultivation, as well as DNA fragments of mycoplasma observed after antibiotic therapy. Studies based on modern microscopy methods showed that microorganisms on the surface of the kidney stone formed multispecies biofilms.

The real work is devoted to studying of a possibility of use of the P-2 rust solvent for preparation of a surface of steel for coloring for the purpose of replacement of ecologically harmful mechanical ways of cleaning and improvement of its protective properties by introduction of organic inhibitors., Исследование посвящено изучению возможности применения преобразователя ржавчины П-2 при подготовке поверхности стали к окрашиванию с целью замены экологически вредных механических способов очистки и улучшения его защитных свойств путем введения органических ингибиторов.