780 results on '"A. Hamabata"'
Search Results
52. A Case of Neurilemmoma Originating in the Hypoglossal Nerve Restricted to the Submandibular Region
- Author
-
Yokoyama, Yuya, primary, Hamabata, Ryoki, additional, Kaida, Hideyuki, additional, and Suga, Kenji, additional
- Published
- 2023
- Full Text
- View/download PDF
53. Severe Hemolytic Jaundice in a Neonate with a Novel COL4A1 Mutation
- Author
-
Seiichi Tomotaki, Hiroshi Mizumoto, Takayuki Hamabata, Akira Kumakura, Mitsutaka Shiota, Hiroshi Arai, Kazuhiro Haginoya, and Daisuke Hata
- Subjects
anemia ,COL4A1 ,hemolysis ,jaundice ,neonate ,type IV collagen ,Pediatrics ,RJ1-570 - Abstract
We report our experience with a preterm infant with severe hemolytic jaundice who required exchange transfusion just after birth. The patient was negative for alloimmune hemolysis as a result of maternal–fetal blood type incompatibility, and tests for inherited defects in erythrocyte metabolism, membrane function, and hemoglobin synthesis were normal. We also performed a bone marrow examination, but could not identify the cause of hemolysis. The patient had several other complications, including porencephaly, epilepsy, elevated serum levels of creatine kinase, and persistent microscopic hematuria. Later, we detected a genetic mutation in COL4A1, which was recently found to be associated with hemolytic anemia. We therefore believe that all of the patient's clinical features, including hemolytic anemia, were due to the mutation in COL4A1. Genetic testing for COL4A1 mutations is recommended in neonates who exhibit hemolytic disease of unknown etiology, especially when other complications compatible with COL4A1-related disorders are present.
- Published
- 2016
- Full Text
- View/download PDF
54. Black pleural effusion
- Author
-
Iori Uchida, Yusuke Ito, Takayuki Hamabata, and Kenichiro Kobayashi
- Subjects
Pediatrics, Perinatology and Child Health - Published
- 2023
55. Endangered island endemic plants have vulnerable genomes
- Author
-
Hamabata, Tomoko, Kinoshita, Gohta, Kurita, Kazuki, Cao, Ping-Lin, Ito, Motomi, Murata, Jin, Komaki, Yoshiteru, Isagi, Yuji, and Makino, Takashi
- Published
- 2019
- Full Text
- View/download PDF
56. Fine-scale genetic population structure of loggerhead turtles in the Northwest Pacific
- Author
-
Matsuzawa, Y, Kamezaki, N, Ishihara, T, Omuta, K, Takeshita, H, Goto, K, Arata, T, Honda, H, Kameda, K, Kashima, Y, Kayo, M, Kawazu, I, Kodama, J, Kumazawa, Y, Kuroyanagi, K, Mizobuchi, K, Mizuno, K, Oki, K, Watanabe, KK, Yamamoto, A, Yamashita, Y, Yamato, T, Hamabata, T, Ishizaki, A, and Dutton, PH
- Subjects
Zoology ,QL1-991 ,Botany ,QK1-989 - Abstract
Effective conservation of globally distributed marine species relies on identification of demographically independent populations to ensure that management actions are directed at the appropriate scale. This identification is particularly challenging for species with complex life histories when local breeding populations have not been adequately sampled. We used mtDNA to analyze the population structure of loggerhead turtles from a total of 555 samples collected from 12 nesting sites in Japan in the Northwest Pacific, including previously unsampled rookeries in the Ryukyu Archipelago, for a comprehensive coverage of the nesting distribution. We identified a total of 9 haplotypes based on 820 bp of the mtDNA control region, including 5 variants of a single previously described 380 bp haplotype. We discovered that 1 haplotype (CcP1.1) previously rare in the North Pacific is common in the Ryukyu Archipelago. Based on analysis of haplotype frequencies, we found significant differentiation among regionally grouped nesting populations (analysis of molecular variance p < 0.0001, df = 8; pairwise FST ranging from 0.033 to 0.145). Our results provide evidence to support the recognition of 3 management units (MU) within the NW Pacific Regional Management Unit (RMU). These include (1) the Ryukyu MU that includes Okinawa, Okinoerabu and Amami, (2) Yakushima Island MU and (3) a Mainland MU that includes Bousou, Enshu-nada, Shikoku, Kii and Eastern Kyushu. These new data from Japan will provide important baseline data for global genetic stock assessments and contribute to our understanding of the population structure, ecology and life history of this migratory marine species in the northern Pacific.
- Published
- 2016
- Full Text
- View/download PDF
57. Halo concentration, galaxy red fraction, and gas properties of optically defined merging clusters
- Author
-
Nobuhiro Okabe, Masamune Oguri, Hiroki Akamatsu, Akinari Hamabata, Atsushi J Nishizawa, Elinor Medezinski, Yusei Koyama, Masao Hayashi, Taizo Okabe, Shutaro Ueda, Ikuyuki Mitsuishi, and Naomi Ota
- Published
- 2019
- Full Text
- View/download PDF
58. Nationwide and long-term molecular epidemiologic studies of mumps viruses that circulated in Japan between 1986 and 2017
- Author
-
Kidokoro, Minoru, Shiino, Teiichiro, Yamaguchi, Tomohiro, Nariai, Eri, Kodama, Hiroe, Nakata, Keiko, Sano, Takako, Gotou, Keiko, Kisu, Tomoko, Maruyama, Tomomi, Kuba, Yumani, Sakata, Wakako, Higashi, Teruaki, Kiyota, Naoko, Sakai, Takashi, Yahiro, Shunsuke, Nagita, Akira, Watanabe, Kaori, Hirokawa, Chika, Hamabata, Hirotsune, Fujii, Yoshiki, Yamamoto, Miwako, Yokoi, Hajime, Sakamoto, Misako, Saito, Hiroyuki, Shibata, Chihiro, Inada, Machi, Fujitani, Misako, Minagawa, Hiroko, Ito, Miyabi, Shima, Akari, Murano, Keiko, Katoh, Hiroshi, Kato, Fumihiro, Takeda, Makoto, and Suga, Shigeru
- Subjects
Microbiology (medical) ,Microbiology - Abstract
In Japan, major mumps outbreaks still occur every 4–5 years because of low mumps vaccine coverage (30–40%) owing to the voluntary immunization program. Herein, to prepare for a regular immunization program, we aimed to reveal the nationwide and long-term molecular epidemiological trends of the mumps virus (MuV) in Japan. Additionally, we performed whole-genome sequencing (WGS) using next-generation sequencing to assess results from conventional genotyping using MuV sequences of the small-hydrophobic (SH) gene. We analyzed 1,064 SH gene sequences from mumps clinical samples and MuV isolates collected from 25 prefectures from 1986 to 2017. The results showed that six genotypes, namely B (110), F (1), G (900), H (3), J (41), and L (9) were identified, and the dominant genotypes changed every decade in Japan since the 1980s. Genotype G has been exclusively circulating since the early 2000s. Seven clades were identified for genotype G using SH sequence-based classification. To verify the results, we performed WGS on 77 representative isolates of genotype G using NGS and phylogenetically analyzed them. Five clades were identified with high bootstrap values and designated as Japanese clade (JPC)-1, -2, -3, -4, -5. JPC-1 and -3 accounted for over 80% of the total genotype G isolates (68.3 and 13.8%, respectively). Of these, JPC-2 and -5, were newly identified clades in Japan through this study. This is the first report describing the nationwide and long-term molecular epidemiology of MuV in Japan. The results provide information about Japanese domestic genotypes, which is essential for evaluating the mumps elimination progress in Japan after the forthcoming introduction of the mumps vaccine into Japan’s regular immunization program. Furthermore, the study shows that WGS analysis using NGS is more accurate than results obtained from conventional SH sequence-based classification and is a powerful tool for accurate molecular epidemiology studies.
- Published
- 2022
- Full Text
- View/download PDF
59. Antigenic Change in Human Influenza A(H2N2) Viruses Detected by Using Human Plasma from Aged and Younger Adult Individuals
- Author
-
Yukimasa Matsuzawa, Kiyoko Iwatsuki-Horimoto, Yoshinori Nishimoto, Yukiko Abe, Satoshi Fukuyama, Taiki Hamabata, Moe Okuda, Yui Go, Tokiko Watanabe, Masaki Imai, Yasumichi Arai, Ron A.M. Fouchier, Seiya Yamayoshi, and Yoshihiro Kawaoka
- Subjects
influenza a virus ,aged individuals ,h2n2 ,antigenic drift ,antigenic change ,Microbiology ,QR1-502 - Abstract
Human influenza A(H2N2) viruses emerged in 1957 and were replaced by A(H3N2) viruses in 1968. The antigenicity of human H2N2 viruses has been tested by using ferret antisera or mouse and human monoclonal antibodies. Here, we examined the antigenicity of human H2N2 viruses by using human plasma samples obtained from 50 aged individuals who were born between 1928 and 1933 and from 33 younger adult individuals who were born after 1962. The aged individuals possessed higher neutralization titers against H2N2 viruses isolated in 1957 and 1963 than those against H2N2 viruses isolated in 1968, whereas the younger adults who were born between 1962 and 1968 possessed higher neutralization titers against H2N2 viruses isolated in 1963 than those against other H2N2 viruses. Antigenic cartography revealed the antigenic changes that occurred in human H2N2 viruses during circulation in humans for 11 years, as detected by ferret antisera. These results show that even though aged individuals were likely exposed to more recent H2N2 viruses that are antigenically distinct from the earlier H2N2 viruses, they did not possess high neutralizing antibody titers to the more recent viruses, suggesting immunological imprinting of these individuals with the first H2N2 viruses they encountered and that this immunological imprinting lasts for over 50 years.
- Published
- 2019
- Full Text
- View/download PDF
60. Shiga toxin 2eB-transgenic lettuce vaccine: N-glycosylation is important for protecting against porcine edema disease
- Author
-
HAMABATA, Takashi, SATO, Toshio, TAKITA, Eiji, MATSUI, Takeshi, KAWABATA, Takahiro, IMAOKA, Taishi, NAKANISHI, Nobuo, TSUKAHARA, Takamitsu, and SAWADA, Kazutoshi
- Subjects
colostrum-deprived piglet infection model ,Glycosylation ,Full Paper ,Shiga-Toxigenic Escherichia coli ,Swine ,animal diseases ,Bacteriology ,porcine edema disease ,Shiga toxin 2e-producing Escherichia coli ,Lettuce ,stx2eB-transgenic lettuce vaccine ,Shiga Toxin 2 ,Shiga Toxin ,fluids and secretions ,Bacterial Vaccines ,Animals ,Edema ,Escherichia coli Infections - Abstract
Porcine edema disease (ED) is a life-threatening toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) in weaned piglets. We previously reported that the stx2eB-transgenic lettuce 2BH strain shows potential for use as an oral vaccine candidate against ED. However, the 2BH strain expressed a hemagglutinin (HA)-tag together with Stx2eB and contained non-canonical N-glycosylation. Therefore, we developed two Stx2eB-lettuce strains, the 3 (G+) strain in which the HA-tag was removed from 2BH, and the 3 (G-) lettuce strain, in which the 73rd Asn was replaced with Ser to prevent non-canonical N-glycosylation of Stx2eB from the 3 (G+) strain. We examined the protective effect of these newly developed two strains compared with the previous 2BH strain against ED using a colostrum-deprived piglet STEC infection model. We found that the N-glycosylated 2BH and 3 (G+) strains relieved the pathogenic symptoms of ED in STEC-challenged piglets, whereas the non-glycosylated 3 (G-) strain did not. N-Glycosylation of the Stx2eB product in lettuce may be involved in the immune response in piglets.
- Published
- 2021
61. Proteasome inhibitors prevent cell death and prolong survival of mice challenged by Shiga toxin
- Author
-
Takayuki Hattori, Miho Watanabe-Takahashi, Nobumichi Ohoka, Takashi Hamabata, Koichi Furukawa, Kiyotaka Nishikawa, and Mikihiko Naito
- Subjects
Shiga toxin ,Apoptosis ,Apoptosis inhibitory proteins ,Proteasome ,Proteasome inhibitor ,Biology (General) ,QH301-705.5 - Abstract
Shiga toxin (Stx) causes fatal systemic complications. Stx induces apoptosis, but the mechanism of which is unclear. We report that Stx induced rapid reduction of short‐lived anti‐apoptotic proteins followed by activation of caspase 9 and the progression of apoptosis. Proteasome inhibitors prevented the reduction of anti‐apoptotic proteins, and inhibited caspase activation and apoptosis, suggesting that the reduction of anti‐apoptotic proteins is a prerequisite for Stx‐induced apoptosis. A clinically approved proteasome inhibitor, bortezomib, prolonged the survival of mice challenged by Stx. These results imply that proteasome inhibition may be a novel approach to prevent the fatal effects of Stx.
- Published
- 2015
- Full Text
- View/download PDF
62. Age-stratified seroprevalence of antibodies against SARS-CoV-2 in the pre- and post-vaccination era, February 2020–March 2022, Japan
- Author
-
Seiya Yamayoshi, Kiyoko Iwatsuki-Horimoto, Moe Okuda, Michiko Ujie, Atsuhiro Yasuhara, Jurika Murakami, Calvin Duong, Taiki Hamabata, Mutsumi Ito, Shiho Chiba, Ryo Kobayashi, Satoshi Takahashi, Keiko Mitamura, Masao Hagihara, Akimichi Shibata, Yoshifumi Uwamino, Naoki Hasegawa, Toshiaki Ebina, Akihiko Izumi, Hideaki Kato, Hideaki Nakajima, Norio Sugaya, Yuki Seki, Asef Iqbal, Isamu Kamimaki, Masahiko Yamazaki, Yoshihiro Kawaoka, and Yuki Furuse
- Abstract
Japan has reported a small number of COVID-19 cases relative to other countries. Because not all infected people receive diagnostic tests for COVID-19, the reported number of COVID-19 cases must be lower than the actual number of infections. Assessments of the presence of antibodies against the spike protein of SARS-CoV-2 can retrospectively determine the history of natural infection and vaccination. In this study, we assessed SARS-CoV-2 seroprevalence by analyzing over 60,000 samples collected in Japan from February 2020 to March 2022. The results showed that about 5% of the Japanese population had been infected with the virus by January 2021. The seroprevalence increased with the administration of vaccinations to adults; however, among the elderly, it was not as high as the vaccination rate, probably due to poor immune responses to the vaccines and waning immunity. The infection was spread during the epidemic waves caused by the SARS-CoV-2 Delta and Omicron variants among children who were not eligible for vaccination. Nevertheless, their seroprevalence was as low as 10% as of March 2022. Our study underscores the low incidence of SARS-CoV-2 infection in Japan and the effects of vaccination on immunity at the population level.
- Published
- 2022
63. Age-stratified seroprevalence of antibodies against SARS-CoV-2 in the pre- and post-vaccination era, February 2020–March 2022, Japan
- Author
-
Yamayoshi, Seiya, primary, Iwatsuki-Horimoto, Kiyoko, additional, Okuda, Moe, additional, Ujie, Michiko, additional, Yasuhara, Atsuhiro, additional, Murakami, Jurika, additional, Duong, Calvin, additional, Hamabata, Taiki, additional, Ito, Mutsumi, additional, Chiba, Shiho, additional, Kobayashi, Ryo, additional, Takahashi, Satoshi, additional, Mitamura, Keiko, additional, Hagihara, Masao, additional, Shibata, Akimichi, additional, Uwamino, Yoshifumi, additional, Hasegawa, Naoki, additional, Ebina, Toshiaki, additional, Izumi, Akihiko, additional, Kato, Hideaki, additional, Nakajima, Hideaki, additional, Sugaya, Norio, additional, Seki, Yuki, additional, Iqbal, Asef, additional, Kamimaki, Isamu, additional, Yamazaki, Masahiko, additional, Kawaoka, Yoshihiro, additional, and Furuse, Yuki, additional
- Published
- 2022
- Full Text
- View/download PDF
64. Evaluation of Linear Axis Motion Error of Machine Tools Using an R-test Device
- Author
-
Masashi, Yamaji, Hamabata, Naoki, and Ihara, Yukitoshi
- Published
- 2014
- Full Text
- View/download PDF
65. Gene expression analysis during the conversion from a viable but nonculturable to culturable state in Vibrio cholerae
- Author
-
Alafate Ayibieke, Ayae Nishiyama, Mitsutoshi Senoh, and Takashi Hamabata
- Subjects
Genetics ,General Medicine - Published
- 2023
66. A nontoxigenic form of Shiga toxin 2 suppresses the production of amyloid β by altering the intracellular transport of amyloid precursor protein through its receptor-binding B-subunit
- Author
-
Satoru Funamoto, Koichi Furukawa, Waka Sato, Takashi Hamabata, Kiyotaka Nishikawa, and Miho Watanabe-Takahashi
- Subjects
0301 basic medicine ,Cell Survival ,Endosome ,Protein subunit ,Biophysics ,CHO Cells ,Endosomes ,Endocytosis ,Shiga Toxin 2 ,Biochemistry ,Amyloid beta-Protein Precursor ,03 medical and health sciences ,symbols.namesake ,Cricetulus ,0302 clinical medicine ,Catalytic Domain ,Extracellular ,Amyloid precursor protein ,Animals ,Humans ,Molecular Biology ,Amyloid beta-Peptides ,Globosides ,biology ,Chemistry ,Trihexosylceramides ,Endoplasmic reticulum ,Cell Membrane ,Shiga toxin ,Cell Biology ,Golgi apparatus ,Recombinant Proteins ,Cell biology ,Protein Transport ,030104 developmental biology ,030220 oncology & carcinogenesis ,Mutation ,Phosphatidylcholines ,symbols ,biology.protein ,Lysosomes - Abstract
Accumulation of amyloid-β peptide (Aβ) in neuronal cells and in the extracellular regions in the brain is a major cause of Alzheimer's disease (AD); therefore, inhibition of Aβ accumulation offers a promising approach for therapeutic strategies against AD. Aβ is produced by sequential proteolysis of amyloid precursor protein (APP) in late/recycling endosomes after endocytosis of APP located in the plasma membrane. Aβ is then released from cells in a free form or in an exosome-bound form. Shiga toxin (Stx) is a major virulence factor of enterohemorrhagic Escherichia coli. Recently, we found that one of the Stx subtypes, Stx2a, has a unique intracellular transport route after endocytosis through its receptor-binding B-subunit. A part of Stx2a can be transported to late/recycling endosomes and then degraded in a lysosomal acidic compartment, although in general Stx is transported to the Golgi and then to the endoplasmic reticulum in a retrograde manner. In this study, we found that treatment of APP-expressing cells with a mutant Stx2a (mStx2a), lacking cytotoxic activity because of mutations in the catalytic A-subunit, stimulated the transport of APP to the acidic compartment, which led to degradation of APP and a reduction in the amount of Aβ. mStx2a-treatment also inhibited the extracellular release of Aβ. Therefore, mStx2a may provide a new strategy to inhibit the production of Aβ by modulating the intracellular transport of APP.
- Published
- 2021
67. MtDNA analysis suggests local origin of pelagic-stage juvenile green turtles collected in Japanese coastal waters
- Author
-
Hamabata, Tomoko, Hikida, Tsutomu, Ishihara, Takashi, Kawazu, Isao, Nashiki, Yukimasa, Oki, Katsuki, Tanaka, Toshiyuki, Ui, Kenjiro, and Kamezaki, Naoki
- Subjects
CRC Press L.L.C. ,Green turtle -- Analysis ,Book publishing -- Analysis ,Mitochondrial DNA -- Analysis ,Earth sciences ,Science and technology - Abstract
Abstract: Understanding the distribution range across the life history of endangered animals is crucial for effective conservation and management planning. However, endangered migratory green turtles with straight carapace lengths (SCLs) [...]
- Published
- 2016
- Full Text
- View/download PDF
68. Juvenile green turtles on the northern edge of their range: mtDNA evidence of long-distance westward dispersals in the northern Pacific Ocean
- Author
-
H Nishizawa, T Narazaki, T Fukuoka, K Sato, T Hamabata, M Kinoshita, and N Arai
- Subjects
Zoology ,QL1-991 ,Botany ,QK1-989 - Abstract
Understanding the dispersal pathway and connectivity of an endangered species plays an essential role in the development of strategies for its effective conservation and management. By using mtDNA control region sequences, we identified the genetic composition and estimated the origin of the northernmost feeding aggregation of green turtles Chelonia mydas around the Sanriku coast of Japan. Significant differences in haplotype frequencies between Sanriku and southern Japanese feeding aggregations, a significant correlation between genetic distance and geographical distance in Japanese feeding aggregations, and estimated contribution to the Sanriku, mainly from the Japanese rookery of Ogasawara, indicate compositional changes from the south to the north along the Japanese Archipelago and suggest that the northern feeding aggregations were occupied by turtles born mainly in Japanese rookeries. However, haplotypes specific or similar to Hawaiian and eastern Pacific rookeries were detected, and substantial contributions from Hawaii or the eastern Pacific to the Sanriku feeding aggregation were estimated. Combined with the observation of specimens with phenotypic features of the subspecies ‘black turtle’ nesting in the eastern Pacific, the results indicate the long-distance dispersal of hatchlings born in Hawaii or the eastern Pacific to Japanese coastal waters, possibly through the North Equatorial Current. Although the level of contribution may be small, this study genetically supports the occurrence of the westward long-distance dispersal of green turtles in the Pacific.
- Published
- 2014
- Full Text
- View/download PDF
69. Genetic composition of loggerhead turtle feeding aggregations: migration patterns in the North Pacific
- Author
-
H Nishizawa, T Narazaki, T Fukuoka, K Sato, T Hamabata, M Kinoshita, and N Arai
- Subjects
Zoology ,QL1-991 ,Botany ,QK1-989 - Abstract
The loggerhead turtle Caretta caretta is highly migratory and undertakes transoceanic migrations. In the North Pacific, loggerhead turtles that hatch on Japanese beaches reach the vicinity of Baja California in the eastern Pacific. As they grow, they return and recruit to the feeding areas around Japan. By using mtDNA control-region sequences, we identified the genetic composition of the feeding aggregation around the Sanriku coastal area (n = 107), >500 km north of the main nesting beaches of Japan and located on the north of the Japanese mainland. Performing a mixed-stock analysis using the published data from 5 Japanese nesting rookeries (n = 279 in total) as sources, the origin of the feeding aggregation was estimated with Bayesian statistics. The results indicated a high contribution from the southern rookeries (mean ≥ 82.10%), mainly the Yakushima rookery (mean ≥ 51.45%), to the Sanriku feeding aggregation, whether the number of nests was considered as an informative prior or not. Therefore, the Sanriku coastal area is estimated to be utilized by loggerhead turtles born in the southern nesting rookeries relatively far from Sanriku. The strong connectivity between loggerheads from the Sanriku feeding aggregation and the southern Japanese rookeries suggests that loggerhead turtles in the North Pacific generally settle in the Japanese coastal areas in the large juvenile stage, but not in the direct vicinity of their natal sites, and some juveniles that use an oceanic feeding strategy are recruited to the Sanriku coastal area.
- Published
- 2014
- Full Text
- View/download PDF
70. Shiga toxin 2eB-transgenic lettuce vaccine: N-glycosylation is important for protecting against porcine edema disease
- Author
-
Toshio Sato, Takashi Hamabata, Kazutoshi Sawada, Nobuo Nakanishi, Kawabata Takahiro, Eiji Takita, Takeshi Matsui, Taishi Imaoka, and Takamitsu Tsukahara
- Subjects
Glycosylation ,General Veterinary ,biology ,Strain (chemistry) ,animal diseases ,Transgene ,Shiga toxin ,Hemagglutinin ,medicine.disease_cause ,Microbiology ,chemistry.chemical_compound ,fluids and secretions ,Immune system ,chemistry ,N-linked glycosylation ,biology.protein ,medicine ,Escherichia coli - Abstract
Porcine edema disease (ED) is a life-threatening toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) in weaned piglets. We previously reported that the stx2eB-transgenic lettuce 2BH strain shows potential for use as an oral vaccine candidate against ED. However, the 2BH strain expressed a hemagglutinin (HA)-tag together with Stx2eB and contained non-canonical N-glycosylation. Therefore, we developed two Stx2eB-lettuce strains, the 3 (G+) strain in which the HA-tag was removed from 2BH, and the 3 (G-) lettuce strain, in which the 73rd Asn was replaced with Ser to prevent non-canonical N-glycosylation of Stx2eB from the 3 (G+) strain. We examined the protective effect of these newly developed two strains compared with the previous 2BH strain against ED using a colostrum-deprived piglet STEC infection model. We found that the N-glycosylated 2BH and 3 (G+) strains relieved the pathogenic symptoms of ED in STEC-challenged piglets, whereas the non-glycosylated 3 (G-) strain did not. N-Glycosylation of the Stx2eB product in lettuce may be involved in the immune response in piglets.
- Published
- 2021
71. Investigation of parotid gland cancer surgery that preserved the facial nerve
- Author
-
Michihiko Sone, Ryoki Hamabata, Nobuaki Mukoyama, Takashi Maruo, Yasushi Fujimoto, Naoki Nishio, Mariko Hiramatsu, and Sayaka Yokoi
- Subjects
Oncology ,Otorhinolaryngology ,business.industry ,Medicine ,Anatomy ,business ,Facial nerve ,Parotid Gland Cancer - Published
- 2021
72. Subclade 2.2.1-Specific Human Monoclonal Antibodies That Recognize an Epitope in Antigenic Site A of Influenza A(H5) Virus HA Detected between 2015 and 2018
- Author
-
Moe Okuda, Seiya Yamayoshi, Ryuta Uraki, Mutsumi Ito, Taiki Hamabata, and Yoshihiro Kawaoka
- Subjects
Influenza A virus ,H5-HA ,human monoclonal antibody ,escape mutant virus ,Microbiology ,QR1-502 - Abstract
Highly pathogenic avian H5 influenza viruses persist among poultry and wild birds throughout the world. They sometimes cause interspecies transmission between avian and mammalian hosts. H5 viruses possessing the HA of subclade 2.3.4.4, 2.3.2.1, 2.2.1, or 7.2 were detected between 2015 and 2018. To understand the neutralizing epitopes of H5-HA, we characterized 15 human monoclonal antibodies (mAbs) against the HA of H5 viruses, which were obtained from volunteers who received the H5N1 vaccine that contains a subclade 2.2.1 or 2.1.3.2 virus as an antigen. Twelve mAbs were specific for the HA of subclade 2.2.1, two mAbs were specific for the HA of subclade 2.1.3.2, and one mAb was specific for the HA of both. Of the 15 mAbs analyzed, nine, which were specific for the HA of subclade 2.2.1, and shared the VH and VL genes, possessed hemagglutination inhibition and neutralizing activities, whereas the others did not. A single amino acid substitution or insertion at positions 144–147 in antigenic site A conferred resistance against these nine mAbs to the subclade 2.2.1 viruses. The amino acids at positions 144–147 are highly conserved among subclade 2.2.1, but differ from those of other subclades. These results show that the neutralizing epitope including amino acids at positions 144–147 is targeted by human antibodies, and plays a role in the antigenic difference between subclade 2.2.1 and other subclades.
- Published
- 2019
- Full Text
- View/download PDF
73. Duration of fever and symptoms in influenza-infected children treated with baloxavir marboxil during the 2019–2020 season in Japan and detection of influenza virus with the PA E23K substitution
- Author
-
Wagatsuma, Keita, primary, Saito, Reiko, additional, Chon, Irina, additional, Phyu, Wint Wint, additional, Fujio, Kakuya, additional, Kawashima, Takashi, additional, Sato, Isamu, additional, Saito, Tadashi, additional, Minato, Michiyoshi, additional, Kodo, Naoki, additional, Suzuki, Eitaro, additional, Ono, Yasuhiko, additional, Masaki, Hironori, additional, Shirahige, Yutaka, additional, Kitano, Akito, additional, Hamabata, Hirotsune, additional, Yuyang, Sun, additional, Jiaming, Li, additional, and Watanabe, Hisami, additional
- Published
- 2022
- Full Text
- View/download PDF
74. Influenza Outbreak and a Group Meal in a Geriatric Long-term Care Facility in Japan
- Author
-
AKIO YOSHIMURA, TEPPEI SASAHARA, RYUSUKE AE, KOKI KOSAMI, DAI AKINE, MASANORI OGAWA, KENJI HAMABATA, SHUJI HATAKEYAMA, YUJI MORISAWA, and LONGZHU CUI
- Subjects
Japan ,Influenza, Human ,Public Health, Environmental and Occupational Health ,Humans ,Applied Microbiology and Biotechnology ,Long-Term Care ,Meals ,Aged ,Disease Outbreaks ,Nursing Homes - Abstract
Influenza outbreaks at geriatric long-term care facilities (g-LTCFs) can be deadly and their prevention is important. However, the factors influencing disease transmission in g-LTCFs remain controversial. In this descriptive study, we tried to identify the potential risk factors influencing influenza outbreaks that occurred in different influenza seasons within a single g-LTCF with 100 residents in Gunma Prefecture. We reviewed the detailed facility records for all influenza cases in both residents and staff between January 2012 and June 2020. Facility preventive measures were also reviewed. We found that community meals may have been a potential source of transmission leading to the outbreaks. When influenza infection is noted, implementation of strict preventive measures and restriction of meal provision to resident rooms may help to prevent disease transmission and the development of an outbreak. Such measures may also be useful to prevent the transmission of other serious droplet-borne diseases within g-LTCFs.
- Published
- 2022
75. Lipid transfer protein syndrome due to wild grape juice and various vegetables: A life‐threatening food allergen
- Author
-
Michiyo Nakagawa, Hiroo Amano, Akemi Hamabata, and Miho Hanada
- Subjects
Male ,Adolescent ,Dermatology ,Cross Reactions ,Immunoglobulin E ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Mugwort ,Vegetables ,Pepper ,medicine ,Humans ,Ingestion ,Vitis ,Food science ,Sensitization ,Plant Proteins ,Skin Tests ,biology ,business.industry ,fungi ,food and beverages ,Long-term potentiation ,General Medicine ,Allergens ,Antigens, Plant ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Carrier Proteins ,business ,Plant lipid transfer proteins ,Food Hypersensitivity ,Salicylic acid - Abstract
We report a 16-year-old Japanese male with mugwort pollinosis in whom ingestion of wild grape juice and various vegetables caused recurrent general urticaria, abdominal pain, dyspnea, and loss of consciousness. A skin prick test demonstrated positive reactions to a wide variety of both non-heated and heated vegetables and fruits such as wild grape, bell pepper, onion, Chinese cabbage, tomato, eggplant, and broccoli. The serum immunoglobulin E levels (ImmunoCAP ISAC® ) for Art v 3, Pla a 3, Pru p 3, and Jug r 3, which are lipid transfer protein (LTP) homologs, were positive but those for Bet v 1 and Bet v 2 were negative. We diagnosed the patient as having LTP syndrome, a condition in which additional factors such as exercise, non-steroidal anti-inflammatory drugs, or alcohol may trigger an allergic reaction. Wild grapes contain a large amount of salicylic acid, and in our patient ingestion of wild grape juice led to immediate loss of consciousness in the absence of exercise. This suggests that salicylic acid contained in fruits and vegetables may act as an important cofactor in LTP syndrome. Although sensitization to LTP is rarely reported in Asia, LTP are considered to be potentially life-threatening food allergens, and therefore warrant attention.
- Published
- 2021
76. Impact of thirteen-valent pneumococcal conjugate vaccine on nasopharyngeal carriage in healthy children under 24 months in Okinawa, Japan
- Author
-
Bin Chang, Takaya Toma, Yoshihisa Nakamura, Hirotsune Hamabata, Makoto Ohnishi, Masaya Miyagi, Hideki Akeda, and Kenji Ameku
- Subjects
Male ,0301 basic medicine ,Microbiology (medical) ,Serotype ,medicine.medical_specialty ,030106 microbiology ,Nasopharyngeal carriage ,Serogroup ,Logistic regression ,medicine.disease_cause ,Pneumococcal Infections ,Pneumococcal conjugate vaccine ,Isolation rate ,Pneumococcal Vaccines ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Risk Factors ,Nasopharynx ,Pneumococcal colonization ,Internal medicine ,Streptococcus pneumoniae ,Humans ,Medicine ,Pharmacology (medical) ,030212 general & internal medicine ,Serotyping ,Vaccines, Conjugate ,Immunization Programs ,business.industry ,Infant ,Infectious Diseases ,Carriage ,Child, Preschool ,Carrier State ,Female ,business ,medicine.drug - Abstract
In November 2013, a 13-valent pneumococcal conjugate vaccine (PCV13) for all infants aged younger than 5 years was incorporated into the Japan national immunization program. An annual survey of nasopharyngeal carriage rates was performed on healthy infants aged 2-24 months from Okinawa, Japan to evaluate the effect of PCV13 on pneumococcal colonization. Of 756 evaluable infants, 203 pneumococcal strains were detected in 193 infants. The overall nasopharyngeal carriage rate was 25.5%, which was not different from our previously reported isolation rate before the introduction of PCV13. The main serotypes of the Streptococcus pneumoniae strains are 15A (18.2%), non-typeable (14.8%), and 15B (11.8%). The carriage rates of pneumococcal strains with 7-valent pneumococcal conjugate vaccine serotypes and PCV13 serotypes were 3.0% and 9.9%, respectively. These values were significantly lower than we reported before the introduction of PCV13. However, the carriage rates of non-PCV13 serotypes have increased. Multivariate logistic regression analysis suggested that siblings and day care attendance are risk factors for pneumococcal carriage.
- Published
- 2020
77. Significant loss of genetic diversity and accumulation of deleterious genetic variation in a critically endangered azalea species, Rhododendron boninense , growing on the Bonin Islands
- Author
-
Akiyo Naiki, Kazuki Kurita, Yuji Isagi, Yoshiteru Komaki, Yoshiaki Kameyama, Satoshi Narita, Toshiaki Kondo, Takashi Makino, Mayu Shibabayashi, Ping Lin Cao, and Tomoko Hamabata
- Subjects
Critically endangered ,Genetic diversity ,Ecology ,Genetic variation ,Plant Science ,Biology ,Ecology, Evolution, Behavior and Systematics ,Azalea - Published
- 2020
78. Interprofessional Work Model for Dementia Care in Hospitals for Community-Based Care
- Author
-
Hideyuki Shiotani, Keiko Sekido, and Kenji Hamabata
- Subjects
Rehabilitation ,Work (electrical) ,Nursing ,Care workers ,medicine.medical_treatment ,media_common.quotation_subject ,medicine ,Dementia ,Psychology ,Community-based care ,Empowerment ,medicine.disease ,media_common - Abstract
In this manuscript the authors have studied interprofessional work model for dementia care in hospitals for community-based care. As present situations and problems of dementia patients in hospitals for community-based care, 8 core categories (19 categories) were extracted and as actual situations of interprofessional work for dementia care, 8 core categories (13 categories) were obtained. The authors examined a function of interprofessional work model and practice contents using these categories. The results revealed that better interprofessional work can be expected by six specialists of nurses rehabilitation specialists, MSW, pharmacists, dietitians and care workers developing dementia care based on “Family handling function” “ADL maintenance and improved function” “Staff member education and empowerment function”.
- Published
- 2020
79. Enhanced production of Shiga toxin 1 in enterohaemorrhagic
- Author
-
Takeshi, Shimizu, Manami, Onuki, Shin, Suzuki, Shinichiro, Hirai, Eiji, Yokoyama, Akio, Matsumoto, and Takashi, Hamabata
- Subjects
Oxygen ,Enterohemorrhagic Escherichia coli ,Bacteriophages ,Shiga Toxin 1 ,Shiga Toxin 2 - Abstract
Enterohaemorrhagic
- Published
- 2021
80. Enhanced production of Shiga toxin 1 in enterohaemorrhagic Escherichia coli by oxygen
- Author
-
Shimizu, Takeshi, primary, Onuki, Manami, additional, Suzuki, Shin, additional, Hirai, Shinichiro, additional, Yokoyama, Eiji, additional, Matsumoto, Akio, additional, and Hamabata, Takashi, additional
- Published
- 2021
- Full Text
- View/download PDF
81. Genetic structure of green turtle (Chelonia mydas) peripheral populations nesting in the northwestern pacific rookeries: evidence for northern refugia and postglacial colonization
- Author
-
Hamabata, Tomoko, Kamezaki, Naoki, and Hikida, Tsutomu
- Subjects
Genetic research ,Population genetics -- Research ,Turtles -- Genetic aspects ,Biological sciences - Abstract
Several green turtle (Chelonia mydas) nesting populations have been reported in the northwestern Pacific region, the northernmost limit of its distribution range. However, the population history in this region as a whole is not well understood. To clarify how the green turtle nesting populations have evolved in the northwestern Pacific region, the genetic composition of mitochondrial DNA control region sequences in the northwestern Pacific was compared with that of the other Pacific populations. We analyzed 302 samples from the northwestern Pacific rookeries, including 78 newly collected samples from rookeries in the Ryukyu Archipelago, Japan (from 24.27°N, 123.76°E to 28.45°N, 129.61°E). Our results revealed that the northwestern Pacific populations consisted of one highly endemic lineage (Clade IV) in the northwestern Pacific rookeries and two other lineages (Clades I and V) which were widely observed in other Pacific populations. We concluded that the highly endemic lineage indicated that a refugial population existed in this region during the Last Glacial Maximum, and the other two lineages indicated that colonization from populations at lower latitudes occurred during interglacial periods. The green turtle nesting populations in the present periphery of their distribution range had been thought to have their origin in colonization from lower latitudes, which served as refugia during glacial periods. However, the present results indicated that the northwestern Pacific peripheral populations have been maintained on the evolutionary timescale of this species and should be treated as long-term conservation resources., Introduction The green turtle (Chelonia mydas) has a circumglobal distribution, occurring throughout tropical and, to a lesser extent, subtropical waters, and is listed as Endangered (EN) by the International Union [...]
- Published
- 2014
- Full Text
- View/download PDF
82. Relation between jet flow energy and pulverization work in gas atomization process
- Author
-
Tadashi FUKUDA, Ryuji MORIMOTO, and Naoto HAMABATA
- Subjects
atomization ,powder metallurgy ,pulverization ,atomizing nozzle ,gas jet ,kinetic energy ,molten metal ,Mechanical engineering and machinery ,TJ1-1570 ,Engineering machinery, tools, and implements ,TA213-215 - Abstract
The relationship between the kinetic energy of spraying medium and pulverization work for forming powder using molten metal in gas atomization process has been experimentally studied, which intends improvements of manufacturing fine powder in atomization process. Compressed air was ejected through 1.0, 1.2, 1.5 mm diameter 6 nozzles forming a reverse conical jet whose apex angle is 30 or 40 degrees. The velocity distribution of the gas jet is measured using Pitot tube attached to a traversing equipment. The kinetic energy and momentum of the gas jet were calculated based on measurements of the gas velocity distribution. The gas jet power at the horizontal plane including the geometrical focus was about 15~25 % of the compressing power and it decreases in downstream direction. With the molten metal tube fitted into the atomizing nozzle, molten tin was poured into the gas jets and the powders were produced by gas atomization. The distribution of the particle size was measured by sieving. Median diameters of the powders decreased in accordance with the gas jet power, though they exhibited a lower limit value in higher power region. The surface energy of the powder which is regarded as the pulverization work was obtained by multiplying the powder surface and the surface tension for each particle size section. The pulverization work to form powder is as small as about 1/1000 of the jet flow energy.
- Published
- 2014
- Full Text
- View/download PDF
83. Design of motion accuracy measurement device with three displacement sensors for machine tool and comparison of its setting method
- Author
-
Masashi YAMAJI, Naoki HAMABATA, and Yukitoshi IHARA
- Subjects
five-axis machining center ,motion accuracy ,test standard ,r-test ,setting method ,Engineering machinery, tools, and implements ,TA213-215 ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
The standard of test method for five-axis machining center is now under discussion by ISO. In its draft standard, the use of a measurement device with three displacement sensor is described in addition to the ball bar device that is well-known as the motion measurement device for machine tools. In this study, a device that was able to take the setting method freely was designed and prototyped. Using the newly-designed device, three setting method installed on the machine were compared. One is of the conventional setting method developed in Europe, another is the method that sensors axes are parallel to the machine axes, the other is that the device is attached on the machine spindle. Since the compared results show little difference, the setting method can be decided by convenience of the easiness of installation on the machine such as centering of the master ball, collision prevention and sensor's cable treatment. The measured results on two five-axis machining centers were also considered. The typical error was that the straightness error motion of linear axes, which is difficult to detect by the ball bar test. Both bidirectional repeatability error and cyclic errors are detected as the straightness error.
- Published
- 2014
- Full Text
- View/download PDF
84. A Nontoxic Chimeric Enterotoxin Adjuvant Induces Protective Immunity in Both Mucosal and Systemic Compartments with Reduced IgE Antibodies
- Author
-
Kweon, Mi-Na, Yamamoto, Masafumi, Watanabe, Fumiko, Tamura, Shinichi, Van Ginkel, Frederik W., Miyauchi, Akira, Takagi, Hiroaki, Takeda, Yoshifumi, Hamabata, Takashi, Fujihashi, Kohtaro, McGhee, Jerry R., and Kiyono, Hiroshi
- Published
- 2002
85. Induction and Resuscitation of Viable but Nonculturable Corynebacterium diphtheriae
- Author
-
Masaaki Iwaki, Akihiko Yamamoto, Takashi Hamabata, Ayae Nishiyama, Keigo Shibayama, and Mitsutoshi Senoh
- Subjects
Microbiology (medical) ,QH301-705.5 ,resuscitation ,Human pathogen ,medicine.disease_cause ,Microbiology ,Viable but nonculturable ,VBNC ,03 medical and health sciences ,transcriptome analysis ,Virology ,morphology ,medicine ,Biology (General) ,Escherichia coli ,030304 developmental biology ,Diphtheria toxin ,Corynebacterium diphtheriae ,0303 health sciences ,biology ,030306 microbiology ,Communication ,RT-qPCR ,Pathogenic bacteria ,biology.organism_classification ,Catalase ,Vibrio cholerae ,biology.protein - Abstract
Many pathogenic bacteria, including Escherichia coli and Vibrio cholerae, can become viable but nonculturable (VBNC) following exposure to specific stress conditions. Corynebacterium diphtheriae, a known human pathogen causing diphtheria, has not previously been shown to enter the VBNC state. Here, we report that C. diphtheriae can become VBNC when exposed to low temperatures. Morphological differences in culturable and VBNC C. diphtheriae were examined using scanning electron microscopy. Culturable cells presented with a typical rod-shape, whereas VBNC cells showed a distorted shape with an expanded center. Cells could be transitioned from VBNC to culturable following treatment with catalase. This was further evaluated via RNA sequence-based transcriptomic analysis and reverse-transcription quantitative PCR of culturable, VBNC, and resuscitated VBNC cells following catalase treatment. As expected, many genes showed different behavior by resuscitation. The expression of both the diphtheria toxin and the repressor of diphtheria toxin genes remained largely unchanged under all four conditions (culturable, VBNC, VBNC after the addition of catalase, and resuscitated cells). This is the first study to demonstrate that C. diphtheriae can enter a VBNC state and that it can be rescued from this state via the addition of catalase. This study helps to expand our general understanding of VBNC, the pathogenicity of VBNC C. diphtheriae, and its environmental survival strategy.
- Published
- 2021
86. Shiga toxin 2eB‐transgenic lettuce vaccine is effective in protecting weaned piglets from edema disease caused by Shiga toxin‐producingEscherichia coliinfection
- Author
-
Keizo Nakayama, Takeshi Matsui, Eiji Takita, Nobuo Nakanishi, Takamitsu Tsukahara, Taishi Imaoka, Takashi Hamabata, Toshio Sato, and Kazutoshi Sawada
- Subjects
Swine ,Transgene ,Administration, Oral ,Virulence ,Edema Disease of Swine ,Weaning ,medicine.disease_cause ,Shiga Toxin 2 ,Microbiology ,03 medical and health sciences ,Ingredient ,Oral administration ,medicine ,Animals ,Escherichia coli ,Escherichia coli Infections ,030304 developmental biology ,0303 health sciences ,Shiga-Toxigenic Escherichia coli ,biology ,0402 animal and dairy science ,Shiga toxin ,04 agricultural and veterinary sciences ,General Medicine ,Lettuce ,040201 dairy & animal science ,Vaccination ,Animals, Newborn ,Bacterial Vaccines ,biology.protein ,General Agricultural and Biological Sciences - Abstract
Porcine edema disease (ED) is a toxemia that is caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) and is associated with high mortality. Since ED occurs most frequently during the weaning period, preweaning vaccination of newborn piglets is required. We developed stx2eB-transgenic lettuce as an oral vaccine candidate against ED and examined its protective efficacy using a piglet STEC infection model. Two serially developed Stx2eB-lettuce strains, 2BN containing ingredient Stx2eB constituting a concentration level of 0.53 mg Stx2eB/g of powdered lettuce dry weight (DW) and 2BH containing ingredient Stx2eB constituting a concentration level of 2.3 mg of Stx2eB/g of powdered lettuce DW, were evaluated in three sequential experiments. Taken the results together, oral administration of Stx2eB-lettuce vaccine was suggested to relieve the pathogenic symptoms of ED in piglets challenged with virulent STEC strain. Our data suggested that Stx2eB-lettuce is a promising first oral vaccine candidate against ED.
- Published
- 2019
87. Endangered island endemic plants have vulnerable genomes
- Author
-
Kazuki Kurita, Tomoko Hamabata, Ping Lin Cao, Jin Murata, Yoshiteru Komaki, Takashi Makino, Gohta Kinoshita, Motomi Ito, and Yuji Isagi
- Subjects
Risk ,Heterozygote ,Plant genetics ,Endangered species ,Medicine (miscellaneous) ,Biology ,Evolutionary ecology ,Genome ,General Biochemistry, Genetics and Molecular Biology ,Article ,Evolutionary genetics ,Contig Mapping ,Species Specificity ,lcsh:QH301-705.5 ,Islands ,Genetic diversity ,Extinction ,Ecology ,Geography ,Human evolutionary genetics ,Conservation biology ,Nucleotides ,Sequence Analysis, RNA ,Endangered Species ,Genetic Variation ,Genomics ,Plants ,lcsh:Biology (General) ,Evolutionary biology ,General Agricultural and Biological Sciences ,Genome, Plant - Abstract
Loss of genetic diversity is known to decrease the fitness of species and is a critical factor that increases extinction risk. However, there is little evidence for higher vulnerability and extinction risk in endangered species based on genomic differences between endangered and non-endangered species. This is true even in the case of functional loci, which are more likely to relate to the fitness of species than neutral loci. Here, we compared the genome-wide genetic diversity, proportion of duplicated genes (PD), and accumulation of deleterious variations of endangered island endemic (EIE) plants from four genera with those of their non-endangered (NE) widespread congeners. We focused on exhaustive sequences of expressed genes obtained by RNA sequencing. Most EIE species exhibited significantly lower genetic diversity and PD than NE species. Additionally, all endangered species accumulated deleterious variations. Our findings provide new insights into the genomic traits of EIE species., 絶滅危惧植物にのみ見られるゲノムの脆弱性を発見. 京都大学プレスリリース. 2019-07-05.
- Published
- 2019
88. Actual Usage of ‘Dou and Donna Questions’ in OPI Trainee's Interviews : Survey from ‘Question Type’
- Author
-
yumiko mochida and shizuka hamabata
- Subjects
Type (biology) ,General Chemical Engineering ,Pedagogy ,Psychology - Published
- 2019
89. Duration of fever and symptoms in influenza-infected children treated with baloxavir marboxil during the 2019–2020 season in Japan and detection of influenza virus with the PA E23K substitution
- Author
-
Keita Wagatsuma, Reiko Saito, Irina Chon, Wint Wint Phyu, Kakuya Fujio, Takashi Kawashima, Isamu Sato, Tadashi Saito, Michiyoshi Minato, Naoki Kodo, Eitaro Suzuki, Yasuhiko Ono, Hironori Masaki, Yutaka Shirahige, Akito Kitano, Hirotsune Hamabata, Sun Yuyang, Li Jiaming, and Hisami Watanabe
- Subjects
Dibenzothiepins ,Pharmacology ,Adolescent ,Fever ,Pyridones ,Triazines ,Morpholines ,Antiviral Agents ,Nucleotidyltransferases ,Influenza A Virus, H1N1 Subtype ,Oseltamivir ,Japan ,Virology ,Influenza, Human ,Humans ,Seasons ,Child - Abstract
Data on the clinical effectiveness of the novel anti-influenza drug baloxavir marboxil (baloxavir) in children remain limited. We conducted an observational study to compare the duration of fever and symptoms between baloxavir- and oseltamivir-treated children infected with influenza A and B. In total, 159 outpatients with influenza A(H1N1)pdm09 or B/Victoria-lineage infections, aged19 years, during the 2019-2020 influenza season in Japan were enrolled and assessed the duration of fever and symptoms using the Kaplan-Meier method and a multivariate Cox proportional hazard regression model. Polymerase acidic (PA) variants were examined before and after baloxavir treatment. In the multivariable analysis, the duration of fever and symptoms was unaltered between the A(H1N1)pdm09 (n = 116) and B/Victoria-lineage (n = 43) groups. Conversely, the fever duration was marginally longer in the oseltamivir-treated group (n = 59) than in the baloxavir group (n = 100) (hazard ratio (HR) = 0.67, p = 0.05); however, the duration of symptoms was unaltered between the two groups (HR = 0.74, p = 0.11). No patient presented PA reduced susceptibility marker(s) before baloxavir treatment in the analyzed groups. The PA/E23K variant was detected in one case (1.5%, 1/66) of A(H1N1)pdm09 after baloxavir treatment. One case (2.0%, 1/50) of A(H1N1)pdm09 with an N295S substitution in neuraminidase was detected following oseltamivir treatment. These results suggested that the duration of fever was likely to be shorter with baloxavir than with oseltamivir, but the difference between influenza A (H1N1)pdm09 and B/Victoria-lineage was unclear. It is important to continue evaluating the clinical effectiveness of baloxavir and monitoring its drug susceptibility to the influenza virus.
- Published
- 2022
90. Reduced aleurone α-amylase production in aged wheat seeds is accompanied by lower levels of high-pl αamylase transcripts and reduced response to gibberellic acid
- Author
-
Bernal-Lugo, Irma, Rodriguez, Mireya, Gavilanes-Ruiz, Marina, and Hamabata, Alberto
- Published
- 1999
91. 5,6-DiHETE attenuates vascular hyperpermeability by inhibiting Ca2+ elevation in endothelial cells
- Author
-
Yuri Tachibana, Taiki Hamabata, Tatsuro Nakamura, Takahisa Murata, and Daiki Horikami
- Subjects
0301 basic medicine ,Male ,Nitric Oxide Synthase Type III ,Inflammation ,lipid mediators ,QD415-436 ,Pharmacology ,Biochemistry ,Permeability ,Proinflammatory cytokine ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Endocrinology ,Mediator ,nitric oxide ,Hydroxyeicosatetraenoic Acids ,medicine ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Phosphorylation ,Research Articles ,Aorta ,calcium ,Chemistry ,vascular biology ,Cell Biology ,Lipid signaling ,030104 developmental biology ,Vasoconstriction ,inflammation ,Human umbilical vein endothelial cell ,medicine.symptom ,030217 neurology & neurosurgery ,Histamine ,Intracellular - Abstract
Although more than 100 lipid metabolites have been identified, their bioactivities remain unknown. In a previous study, we discovered that the production of several lipid metabolites in the intestines dramatically changed in colitis. Of these metabolites, 5,6-dihydroxyeicosatetraenoic acid (DiHETE) possesses novel anti-inflammatory activity in the vasculature. In this study, we used mouse and human umbilical vein endothelial cell (HUVEC) models to elucidate the mechanisms underlying the vascular activity of lipid metabolites, particularly those related to the release of histamine, a major proinflammatory mediator that stimulates endothelial cells to produce NO, a mediator of vascular relaxation and hyperpermeability, by activating intracellular Ca(2+) concentration-dependent signaling. In a mouse ear, the administration of 5,6-DiHETE did not induce inflammatory reactions, and pretreatment with 5,6-DiHETE inhibited histamine-induced inflammation, specifically vascular dilation and hyperpermeability. In an isolated mouse aorta, 5,6-DiHETE treatment did not influence vascular contraction but attenuated acetylcholine-induced vascular relaxation. In HUVECs, treatment with 5,6-DiHETE inhibited histamine-induced endothelial barrier disruption and inhibited the production of NO. Most notably, 5,6-DiHETE inhibited histamine-induced increases in intracellular Ca(2+) concentrations in HUVECs. Our findings suggest that 5,6-DiHETE attenuates vascular hyperpermeability during inflammation by inhibiting endothelial Ca(2+) elevation, which might lead to a novel pharmacological strategy against inflammatory diseases.
- Published
- 2018
92. A型インフルエンザウイルスのHA蛋白質に保存されたエピトープに結合するヒトモノクローナル抗体の性状解析
- Author
-
Hamabata, Taiki
- Abstract
学位の種別: 課程博士, 審査委員会委員 : (主査)東京大学教授 俣野 哲朗, 東京大学教授 川口 寧, 東京大学教授 新藏 礼子, 東京大学教授 四柳 宏, 東京大学准教授 堤 武也
- Published
- 2021
93. Author Correction: Pluripotent stem cell model of Shwachman–Diamond syndrome reveals apoptotic predisposition of hemoangiogenic progenitors
- Author
-
Itaru Kato, Akira Shimada, Katsutsugu Umeda, Yasuhiko Kamikubo, Tomoo Daifu, Shiro Baba, Hidefumi Hiramatsu, Mitsujiro Osawa, Seishiro Nodomi, Kenichiro Watanabe, Yoshiko Hashii, Tatsutoshi Nakahata, Keisuke Okita, Souichi Adachi, Junko Takita, Takayuki Tanaka, Kenji Osafune, Toshio Heike, Megumu K. Saito, Akira Niwa, Hiroyoshi Watanabe, Takayuki Hamabata, Satoshi Saida, and Kagehiro Kouzuki
- Subjects
Shwachman–Diamond syndrome ,Multidisciplinary ,Apoptosis ,Science ,medicine ,Cancer research ,Medicine ,Progenitor cell ,Biology ,medicine.disease ,Induced pluripotent stem cell - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2021
94. Interprofessional Work Model for Dementia Care in Hospitals for Community-Based Care
- Author
-
Hamabata, Kenji
- Published
- 2021
95. Understanding X-ray and optical selection of galaxy clusters: A comparison of the XXL and CAMIRA cluster catalogues obtained in the common XXL-HSC SSP area
- Author
-
Keiichi Umetsu, Atsushi J. Nishizawa, L. Chiappetti, Akinari Hamabata, Emanuela Pompei, Bruno Altieri, Elias Koulouridis, P. S. Corasaniti, Paul Giles, Sotiria Fotopoulou, Taizo Okabe, Sinan Aliş, Florian Pacaud, S. Paltani, M. Lieu, C. Garrel, Patrick Valageas, Marguerite Pierre, J. P. Willis, Ben J Maughan, T. Sadibekova, J. Lefevre, Miriam E. Ramos-Ceja, F. Gastaldello, Masamune Oguri, Mauro Sereno, Manolis Plionis, C. Adami, Stefano Ettori, Dominique Eckert, L. Faccioli, Bianca M. Poggianti, Nobuhiro Okabe, Yen-Ting Lin, Laboratoire d'Astrophysique de Marseille (LAM), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Univers et Théories (LUTH (UMR_8102)), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Institut d'Astrophysique de Paris (IAP), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Astrophysique Interprétation Modélisation (AIM (UMR_7158 / UMR_E_9005 / UM_112)), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7), Institut de Recherches sur les lois Fondamentales de l'Univers (IRFU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut de Physique Théorique - UMR CNRS 3681 (IPHT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Astrophysique Interprétation Modélisation (AIM (UMR7158 / UMR_E_9005 / UM_112)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[PHYS]Physics [physics] ,Physics ,Brightness ,Cosmology and Nongalactic Astrophysics (astro-ph.CO) ,010308 nuclear & particles physics ,media_common.quotation_subject ,FOS: Physical sciences ,Astronomy and Astrophysics ,Cosmological model ,Astrophysics ,01 natural sciences ,Universe ,Space and Planetary Science ,Sky ,galaxies: clusters: general ,0103 physical sciences ,Cluster (physics) ,clusters: general [galaxies] ,Cluster sampling ,[PHYS.ASTR]Physics [physics]/Astrophysics [astro-ph] ,010303 astronomy & astrophysics ,Galaxy cluster ,Selection (genetic algorithm) ,media_common ,Astrophysics - Cosmology and Nongalactic Astrophysics - Abstract
Large samples of galaxy clusters provide knowledge of both astrophysics in the most massive virialised environments and the properties of the cosmological model that defines our Universe. However, an important issue that affects the interpretation of galaxy cluster samples is the role played by the selection waveband and the potential for this to introduce a bias in the physical properties of clusters thus selected. We aim to investigate waveband-dependent selection effects in the identification of galaxy clusters by comparing the X-ray Multi-Mirror (XMM) Ultimate Extra-galactic Survey (XXL) and Subaru Hyper Suprime-Cam (HSC) CAMIRA cluster samples identified from a common 22.6 deg2 sky area. We compare 150 XXL and 270 CAMIRA clusters in a common parameter space defined by X-ray aperture brightness and optical richness. We find that 71/150 XXL clusters are matched to the location of a CAMIRA cluster, the majority of which (67/71) display richness values N>15 that exceed the CAMIRA catalogue richness threshold. We find that 67/270 CAMIRA clusters are matched to the location of an XXL cluster (defined within XXL as an extended X-ray source). Of the unmatched CAMIRA clusters, the majority display low X-ray fluxes consistent with the lack of an XXL counterpart. However, a significant fraction (64/107) CAMIRA clusters that display high X-ray fluxes are not asociated with an extended source in the XXL catalogue. We demonstrate that this disparity arises from a variety of effects including the morphological criteria employed to identify X-ray clusters and the properties of the XMM PSF., 15 pages, 14 figures, MNRAS accepted
- Published
- 2021
96. Lipid transfer protein syndrome due to wild grape juice and various vegetables: A life‐threatening food allergen
- Author
-
Hanada, Miho, primary, Nakagawa, Michiyo, additional, Hamabata, Akemi, additional, and Amano, Hiroo, additional
- Published
- 2021
- Full Text
- View/download PDF
97. A nontoxigenic form of Shiga toxin 2 suppresses the production of amyloid β by altering the intracellular transport of amyloid precursor protein through its receptor-binding B-subunit
- Author
-
Sato, Waka, primary, Watanabe-Takahashi, Miho, additional, Hamabata, Takashi, additional, Furukawa, Koichi, additional, Funamoto, Satoru, additional, and Nishikawa, Kiyotaka, additional
- Published
- 2021
- Full Text
- View/download PDF
98. Induction and Resuscitation of Viable but Nonculturable Corynebacterium diphtheriae
- Author
-
Hamabata, Takashi, primary, Senoh, Mitsutoshi, additional, Iwaki, Masaaki, additional, Nishiyama, Ayae, additional, Yamamoto, Akihiko, additional, and Shibayama, Keigo, additional
- Published
- 2021
- Full Text
- View/download PDF
99. Development of cycling probe based real-time PCR methodology for influenza A viruses possessing the PA/I38T amino acid substitution associated with reduced baloxavir susceptibility
- Author
-
Osada, Hidekazu, primary, Chon, Irina, additional, Phyu, Wint Wint, additional, Wagatsuma, Keita, additional, Nagata, Nobuo, additional, Kawashima, Takashi, additional, Sato, Isamu, additional, Saito, Tadashi, additional, Kodo, Naoki, additional, Masaki, Hironori, additional, Asoh, Norichika, additional, Tsuchihashi, Yoshiko, additional, Shirahige, Yutaka, additional, Ono, Yasuhiko, additional, Shimada, Yasushi, additional, Hamabata, Hirotsune, additional, Saito, Kousuke, additional, and Saito, Reiko, additional
- Published
- 2021
- Full Text
- View/download PDF
100. Antibody titers against SARS-CoV-2 decline, but do not disappear for several months
- Author
-
Hiroaki Mitsuya, Takayuki Ogura, Shiho Chiba, Jurika Murakami, Michiko Koga, Junichi Ochi, Masanori Nojima, Ichiro Nakachi, Tetsuya Suzuki, Hiroyuki Nagai, Kenji Maeda, Norio Omagari, Kensuke Fujita, Makoto Saito, Osamu Akasaka, Rie Baba, Hideaki Nakajima, Yoshihiro Kawaoka, Hideaki Kato, Kazuma Yagi, Norio Sugaya, Kiyoko Iwatsuki-Horimoto, Eisuke Adachi, David J. Pattinson, Taiki Hamabata, Mutsumi Ito, Yusuke Miyazato, Keiko Mitamura, Hiroshi Yotsuyanagi, Shin ichiro Hattori, Atsuhiro Yasuhara, Morio Nakamura, Shinya Yamamoto, Moe Okuda, and Seiya Yamayoshi
- Subjects
lcsh:R5-920 ,Allergy ,biology ,business.industry ,010102 general mathematics ,Antibody titer ,General Medicine ,medicine.disease ,01 natural sciences ,03 medical and health sciences ,Titer ,0302 clinical medicine ,Ectodomain ,Immunology ,Cohort ,biology.protein ,Medicine ,030212 general & internal medicine ,0101 mathematics ,Antibody ,lcsh:Medicine (General) ,business ,Neutralizing antibody ,Pathogen ,Research Paper - Abstract
Background To develop an effective vaccine against a novel viral pathogen, it is important to understand the longitudinal antibody responses against its first infection. Here we performed a longitudinal study of antibody responses against SARS-CoV-2 in symptomatic patients. Methods Sequential blood samples were collected from 39 individuals at various timepoints between 0 and 154 days after onset. IgG or IgM titers to the receptor binding domain (RBD) of the S protein, the ectodomain of the S protein, and the N protein were determined by using an ELISA. Neutralizing antibody titers were measured by using a plaque reduction assay. Findings The IgG titers to the RBD of the S protein, the ectodomain of the S protein, and the N protein peaked at about 20 days after onset, gradually decreased thereafter, and were maintained for several months after onset. Extrapolation modeling analysis suggested that the IgG antibodies were maintained for this amount of time because the rate of reduction slowed after 30 days post-onset. IgM titers to the RBD decreased rapidly and disappeared in some individuals after 90 days post-onset. All patients, except one, possessed neutralizing antibodies against authentic SARS-CoV-2, which they retained at 90 days after onset. The highest antibody titers in patients with severe infections were higher than those in patients with mild or moderate infections, but the decrease in antibody titer in the severe infection cohort was more remarkable than that in the mild or moderate infection cohort. Interpretation Although the number of patients is limited, our results show that the antibody response against the first SARS-CoV-2 infection in symptomatic patients is typical of that observed in an acute viral infection. Funding The Japan Agency for Medical Research and Development and the National Institutes of Allergy and Infectious Diseases.
- Published
- 2020
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.