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Purpose Epstein-Barr virus (EBV) is crucial for the pathogenesis of classical Hodgkin lymphoma (CHL). In this study, we evaluated whether the EBV could predict the clinical behavior of CHL and impact the tumor microenvironment(TME) signatures. Methods We conducted immunohistochemical analyses for the expression of EBV-latent membrane protein 1 (LMP1) and markers reflecting immune cells, including CD3, CD4, CD8, FOXP3, CD20, and CD68 in 102 CHL samples and calculated the ratios between cell subsets for each specimen. Results EBV-LMP1 positivity was detected in 35 (34%) cases and remained an independent poor prognostic factor for overall survival (OS) (p = 0.022). In addition, the CD8/FOXP3+ ratio was significantly increased in LMP1+ than in LMP1- tumors (p = 0.027). Using Cox modeling, a high CD8/CD68+ ratio was associated with shorter OS in LMP1+ tumors compared to a low CD8/CD68+ ratio (p = 0.039); however, neither CD8+ nor CD68+ separately was correlated to the CHL survival. Moreover, the Kaplan-Meier method showed an association of a high CD8/CD68+ ratio with inferior OS in LMP1+ tumors (p = 0.013). In multivariate analysis, a high CD8/CD68+ ratio and B-symptoms tended to be independent prognostic factors for OS (HR = 9.80 (0.56-171.5), p = 0.11; HR = 10.28 (1.00-106.0), p= 0.05; respectively). Conclusion EBV-LMP1+ is an independent unfavorable prognostic factor for CHL, modulating the TME towards a cytotoxic profile. Besides, CD8/CD68+ ratio could be a potential factor acccruately predicting the clinical outcomes of LMP1+ CHL patients, providing more prgnostic information than each cell seperately.

This paper investigates the using potential of acryl-functionalized kraft lignin (AKL) in reducing the flammability of polymer composites based on recycled unsaturated polyester resins (UPR). Acryl functionalization of kraft lignin was performed by direct esterification of free polyphenolic groups with acryloyl chloride, after what, the AKL was blended in UPR resin synthesized from the polyols obtained by catalytic depolymerization of waste poly(ethylene terephthalate). The AKL was homogenized in UPR resin in different weight ratios: 2.5, 5.0, 7.5, and 12.5 wt.%. Structural and dynamic-mechanical characteristics of acryl-functionalized kraft lignin and composites were determined using FTIR spectroscopy, dynamicmechanical analysis (DMA), and tensile tests. The influence of functionalization and mass fraction of AKL on tensile and thermal properties of UPR resin was studied. The thermal properties of the composite were tested according to the standard UL-94 method, based on which the highest category of heat-resistant materials is a composite with 12.5 wt.% acylfunctionalized lignin.

The management of obesity is difficult with many failures of lifestyle measures, hence the need to broaden the range of treatments prescribed. The aim of our work was to study the influence of pre and probiotics on weight loss psychological profile and metabolic parameters in obese patients.It is a clinical trial involving 45 obese patients, recruited from the Obesity Unit of the National Institute of Nutrition between March and August 2022 divided into three groups: diet only (low-carbohydrate and reduced energy diet), prebiotics (30 g of carob/day) and probiotics (one tablet containing Bifidobacterium longum, Lactobacillus helveticus, Lactococcus lactis, Streptococcus thermophilus/day). The three groups were matched for age, sex and BMI. Patients were seen after 1 month from the intervention. Anthropometric measures, biological parameters, dietary survey and psychological scores were performed.The average age of our population was 48.73 ± 7.7 years, with a female predominance. All three groups showed a significant decrease in weight, BMI and waist circumference with p .05. Only the prebiotic and probiotic group showed a significant decrease in fat mass (p = .001) and a significant increase in muscle strength with p = .008 and .004, but the differences were not significant between the three groups. Our results showed also a significant decrease in insulinemia and HOMA-IR in the prebiotic group compared to the diet-alone group (p = .03; p = .012) and the probiotic group showed a significant decrease in fasting blood glucose compared to the diet alone group (p = .02). A significant improvement in sleep quality was noted in the prebiotic group (p = .02), with a significant decrease in depression, anxiety and stress in all three groups.The prescription of prebiotics and probiotics with the lifestyle measures seems interesting for the management of obesity especially if it is sarcopenic, in addition to the improvement of metabolic parameters and obesity-related psychiatric disorders.

Signal transducers and activators of transcription 5a and 6 (STAT5a and STAT6) play a critical role in tumorigenesis of mammary glands. Based on previous studies, the breast cancer is largely dependent on hormone receptors. Consequently, it is very interesting to decipher the relationship between the STAT5a and STAT6 expression and the molecular distribution of estrogen receptors (ERs) and progesterone receptors (PRs) in mammary tumors.Our study analyzed the expression of STAT5a and STAT6, ERα, ERβ and PR in 40 breast tumor tissues using quantitative real-time polymerase chain reaction (qRT-PCR). Furthermore, the Ki-67 and HER2 status were detected using immunohistochemistry.STAT5a and STAT6 were retained in the majority of the cases studied. Increasing of STAT5a and STAT6 is significantly associated with ERs and PR. The coexpression of both STAT5a and STAT6 with ERs and PR is associated with high tumor grades. Moreover, the coexpression of STAT5a and STAT6 with ERα and PR is associated with a high proliferation index. In addition, (STAT6 + ERβ+) and (STAT6 + PR+) breast cancer subgroups are associated with lymph node infiltration (P = 0.001 and P = 0.03, respectively).Our study results provide an interaction between STAT5a and STAT6 with ERs and PR inducing cell proliferation. Coexpression of STAT5a and STAT6 with ERs and PR can predict sensibility to hormonal therapy.