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Abstract In this paper we analyse how populist leaders in UK and Italy – namely Nigel Farage, Nicola Sturgeon, Giorgia Meloni, and Matteo Salvini – reacted to the first and second lockdowns on their Twitter accounts, communicating directly to their people. The analysis was carried out following a combined qualitative and quantitative approach to discourse. The qualitative analysis focused on comparing populist leaders’ rhetorical and semiotic choices, while the quantitative analysis observed the response by the community of common Twitter users. This work aims on the one hand at unveiling the strategic use of social media by populist leaders and on the other at investigating conflictual interactional dynamics, especially in times of crisis.

Rapid urbanization and climate change are intensifying the urban heat island (UHI) phenomenon across cities worldwide. There is a pressing need to implement evidence-based mitigation and adaptation strategies as well as to develop tools for effectively measuring the impact of such actions on UHI patterns. In this context, the Local Climate Zone (LCZ) concept is a well-established classification system commonly used for the assessment of UHI. With this in mind, we present here the LCZ-ODC project aiming to develop a methodology for LCZ mapping in the Metropolitan City of Milan (northern Italy) by leveraging multiple geospatial data and cutting-edge software tools, including the Open Data Cube (ODC). A key aim of the project is to develop user-oriented solutions facilitating the exploitation of the generated LCZ maps for different application tasks. In this paper, we first present a brief overview of the methodologies and data sources used in the literature for LCZ mapping. Then, we introduce the LCZ-ODC project, with a focus on the end-user requirements which were gathered through a questionnaire distributed to a sample of potential stakeholders. The primary objective of the survey was to collect insights and consolidate requirements related to the key features of LCZ maps that will be produced within the project. The outcomes of the survey play a pivotal role in guiding the project’s development phase, ensuring that the project outputs will effectively address the identified end-user needs.

In this paper, an assessment of the natural radioactivity level, radon exhalation, metal contamination, and mineralogy of a granodiorite rock sample from Stilo, in the Calabria region, Southern Italy is presented as a case study. This rock was employed as a building material in the area under study. The specific activity of 226Ra, 232Th and 40K natural radioisotopes was assessed through high-purity germanium (HPGe) gamma-ray spectrometry. Then, several indices such as the absorbed gamma dose rate (D), the annual effective dose equivalent (AEDE), the activity concentration index (ACI) and the alpha index (Iα), were quantified to determine any potential radiological health risk related to radiation exposure from the analyzed rock. Furthermore, E-PERM electret ion chambers and inductively coupled plasma mass spectrometry (ICP-MS) measurements were carried out to properly quantify the radon exhalation rate and any possible metal pollution, respectively. In particular, to further address metal pollution factors, the geo-accumulation index (Igeo) was calculated to properly address the toxicity levels of the ecosystem originating from the detected metals. Finally, with the aim of successfully discriminating the provenance of such naturally occurring radionuclides, a combined approach involving X-ray diffraction (XRD) and µ-Raman spectroscopy was employed for the identification of the main radioisotope-bearing minerals characterizing the investigated granodiorite. The results achieved in this case study can be taken as the basis for further inquiries into background levels of radioactivity and chemical contamination in natural stone employed as building materials.

In this article the evaluation of the radon content in drinking water samples from the province of Reggio Calabria (Calabria, Southern Italy), is reported. In particular, the 222Rn activity concentration was measured by using the Liquid Scintillation Counting (LSC) PerkinElmer Tricarb 4910 TR setup, and it was compared with the parameter value (100 Bq L^(-1)) reported in the Italian Legislative Decree 28/2016. The annual effective dose for the population of the investigated areas, due to the ingestion of 222Rn dissolved in water, was then evaluated only in those cases in which the parameter value was exceeded. Noteworthy, results reported in this paper represent a main reference for the investigated areas and constitute a baseline for future investigations regarding background radioactivity levels.

Since the outbreak of COVID-19, researchers have been working tirelessly to discover effective ways to combat coronavirus infection. The use of computational drug repurposing methods and molecular docking has been instrumental in identifying compounds that have the potential to disrupt the binding between the spike glycoprotein of SARS-CoV-2 and human ACE2 (hACE2). Moreover, the pseudovirus approach has emerged as a robust technique for investigating the mechanism of virus attachment to cellular receptors and for screening targeted small molecule drugs. Pseudoviruses are viral particles containing envelope proteins, which mediate the virus’s entry with the same efficiency as that of live viruses but lacking pathogenic genes. Therefore, they represent a safe alternative to screen potential drugs inhibiting viral entry, especially for highly pathogenic enveloped viruses. In this review, we have compiled a list of antiviral plant extracts and natural products that have been extensively studied against enveloped emerging and re-emerging viruses by pseudovirus technology. The review is organized into three parts: (1) construction of pseudoviruses based on different packaging systems and applications; (2) knowledge of emerging and re-emerging viruses; (3) natural products active against pseudovirus-mediated entry. One of the most crucial stages in the life cycle of a virus is its penetration into host cells. Therefore, the discovery of viral entry inhibitors represents a promising therapeutic option in fighting against emerging viruses.

ABSTRACTIncreasing evidence suggests that the beta genus of human papillomavirus (β-HPV) cooperates with ultraviolet B (UVB) radiation in inducing non-melanoma skin cancer (NMSC). For instance, expression of E6 and E7 oncoproteins from cutaneous human papillomavirus type 38 (HPV38) in a transgenic mouse model (Tg38) leads to the development of squamous cell carcinoma (SCC) after chronic UVB exposure. Transcriptomic analysis of the Tg38 SCC samples revealed a deregulation of inflammasome pathway-related genes such as IL-18, which is consistent with the well-known capability of several oncogenic viruses to modulate the immune response, in order to efficiently produce new progeny. However, the mechanism at the gene expression level by which UVB and HPV38 synergize and modulate the innate immune response has not been elucidated yet. Here, we propose a new molecular mechanism for the regulation of the IL-18 promoter that involves direct binding of p53 to specific response elements. This mechanism seems to be impaired in the presence of the β-HPV38 oncoproteins. Instead, a newly described inhibitory complex formed by DNA methyltransferase 1 (DNMT1)/PKR/ΔNp73α is recruited to the region formerly occupied by p53 in primary keratinocytes. Together, these results corroborate our hypothesis that β-HPV38 plays an important role in the inhibition of the ultraviolet-induced inflammasome response. This downregulation is likely to impair the ability of keratinocytes to recruit the immune cell population at the UVB-exposed area, causing a defect in the clearance of cells harboring DNA damage and promoting a favorable environment for the progression of NMSC.IMPORTANCEHere, we demonstrate that the direct binding of p53 on the IL-18 promoter region regulates its gene expression. However, the presence of E6 and E7 from human papillomavirus type 38 impairs this mechanism via a new inhibitory complex formed by DNA methyltransferase 1 (DNMT1)/PKR/ΔNp73α, which binds to the region formerly occupied by p53 in primary keratinocytes.

BackgroundVirtual reality (VR) adventure games can offer ideal technological solutions for training social skills in adolescents with autism spectrum disorder (ASD), leveraging their support for multisensory and multiplayer interactions over distance, which may lower barriers to training access and increase user motivation. However, the design of VR-based game environments for social skills training is still understudied and deserves the deployment of an inclusive design approach to ensure its acceptability by target users. ObjectiveWe aimed to present the inclusive design process that we had followed to develop the Zentastic VR adventure game to foster social skills training in adolescents with ASD and to investigate its feasibility as a training environment for adolescents. MethodsThe VR game supports multiplayer training sessions involving small groups of adolescents and their therapists, who act as facilitators. Adolescents with ASD and their therapists were involved in the design and in an explorative acceptability study of an initial prototype of the gaming environment, as well as in a later feasibility multisession evaluation of the VR game final release. ResultsThe feasibility study demonstrated good acceptability of the VR game by adolescents and an enhancement of their social skills from baseline to posttraining. ConclusionsThe findings provide preliminary evidence of the benefits that VR-based games can bring to the training of adolescents with ASD and, potentially, other neurodevelopmental disorders.

IntroductionVerbal language is one of the most immediate and significant means parents have to express affect and information to their children. Parental speech directed to children has been thoroughly examined in typical development. However, the characteristics of parental speech directed to children with neurodevelopmental disorders are far less well documented, and no recent studies have been carried out that involve autistic1 children and their fathers. Therefore, the present study aims to analyze and compare maternal and paternal speech directed to young autistic children, focusing on fathers’ elements of speech in comparison with maternal language.MethodsN = 88 dyads participated in this study. 44 autistic children (41 males and 3 females) (chronological age: M = 40.01 months; SD = 11.96) in interaction with their fathers (paternal age M = 41.84 years; SD = 7.02) and the same 44 children in interaction with their mothers (maternal age M = 37.37 years; SD = 5.45). The language was verbatim transcribed using ELAN software (ELAN Version 6.4, 2022) and coded with an observational tool (Penman) for analyzing functions and referents of speech after reaching a satisfactory level of agreement between two independent transcribers.ResultsNo differences emerged considering the affective aspects of speech. However, mothers seem to direct more informative salient statements (W = 1,259; p = 0.02) and call the child’s attention more often than fathers (W = 1,253.5; p = 0.02). Regarding referents of informative speech, fathers focused more on the child’s internal states rather than mothers (W = 727; p = 0.04).DiscussionThese results reveal that fathers seem to display a relationship-based approach focused on a non-intrusive style with few demands while talking with their children, providing a complementary role to mothers that allows complete and harmonious stimulation of all areas of child development.

In this study, we investigated whether the difficulties in body motion (BM) perception may led to deficit in emotion recognition in Autism spectrum disorder (ASD). To this aim, individuals with high-functioning ASD were asked to recognise fearful, happy, and neutral BM depicted as static images or dynamic point-light and full-light displays. Results showed slower response times in participants with ASD only in recognising dynamic stimuli, but no group differences in accuracy. This suggests that i) a deficit in action chaining mechanism in ASD may prevent the recognition of dynamic BM automatically and rapidly, ii) individuals with ASD and high cognitive resources can develop alternative--but equally successful--strategies to recognise emotional body expressions. Implications for treatment are discussed

In this article, an investigation into the natural radioactivity content in natural inorganic pigments was carried out, together with the assessment of the radiological health risk for the public related to external exposure to ionizing radiations, via High-Purity Germanium (HPGe) γ-ray spectrometry measurements and the calculation of several indices like the absorbed γ-dose rate (D), the annual effective dose equivalent outdoor (AEDEout) and indoor (AEDEin), and the activity concentration index (I). From the obtained results, it was possible to reasonably exclude radiological hazard effects. In addition, Pearson’s correlation, principal component analysis (PCA), and hierarchical cluster analysis (HCA) were carried out with the aim of determining correlations between natural radioactivity content and radiological indices and with the analyzed samples. As a result, five clusters of the investigated pigments were recognized at the highest level of detail based on their chemical composition and mineralogical nature.

The continuous emergence of SARS-CoV-2 variants caused the persistence of the COVID-19 epidemic and challenged the effectiveness of the existing vaccines. The viral proteases are the most attractive targets for developing antiviral drugs. In this scenario, our study explores the use of HIV-1 protease inhibitors against SARS-CoV-2. An in silico screening of a library of HIV-1 proteases identified four anti-HIV compounds able to interact with the 3CLpro of SARS-CoV-2. Thus, in vitro studies were designed to evaluate their potential antiviral effectiveness against SARS-CoV-2. We employed pseudovirus technology to simulate, in a highly safe manner, the adsorption of the alpha (α-SARS-CoV-2) and omicron (ο-SARS-CoV-2) variants of SARS-CoV-2 and study the inhibitory mechanism of the selected compounds for cell–virus interaction. The results reported a mild activity against the viral proteases 3CLpro and PLpro, but efficient inhibitory effects on the internalization of both variants mediated by cathepsin B/L. Our findings provide insights into the feasibility of using drugs exhibiting antiviral effects for other viruses against the viral and host SARS-CoV-2 proteases required for entry.

The capacity of the Child Behavior Checklist 1½-5 (CBCL 1½-5) to identify children with autism spectrum disorder (ASD) at 18 months was tested on 37 children clinically referred for ASD and 46 children at elevated likelihood of developing ASD due to having an affected brother/sister. At 30 months the clinically referred children all received a confirmatory diagnosis, and 10 out of 46 siblings received a diagnosis of ASD. CBCL 1½-5 profiles were compared with a group of matched children with typical development (effect of cognitive level controlled for). The capacity of the CBCL 1½-5 DSM Oriented-Pervasive Developmental Problems scale to differentiate correctly between children diagnosed with ASD and children with typical development appeared dependent on group ascertainment methodology.

Therapeutic Area: Nutrition/Exercise Background: The Standard American Diet (SAD), typically poor in nutritional value, is a known contributor to cardiometabolic syndromes and cardiovascular disease. Suboptimal nutrition composed of processed and red meat, refined carbohydrates, high salt and added sugars contributes to cardiovascular death and premature mortality. Dietary patterns that focus on plant-based foods are associated with reductions and slowing progression of cardiovascular disease . Specifically, the Mediterranean Diet (MD) has a strong association with lower rates of heart disease, cancer, and obesity while increasing longevity. In recent years, a plant based diet (PBD) has also emerged as a nutritional alternative to the MD with health benefits.The growing knowledge of the importance of well-balanced diet to cardiovascular health has become more well-known as it is a component of the AHA's Life Essential 8, which illustrates the factors that are important to cardiovascular health. Dietary changes are traditionally focused in the outpatient setting, though little has been done in the acute care setting when patients are most vulnerable and amenable to change, especially after a life altering event. Methods: We will recruit adults >18 years of age who are admitted to Temple University Hospital with a standard cardiac diet order with one of the following diagnoses: acute coronary syndrome, heart failure, atrial fibrillation/flutter or hypertensive crisis. Patients will be given a baseline questionnaire to assess dietary behaviors prior to hospital admission.Patients will then be randomized to either a cardiac, PBD or MD. The PBD and MD were created for this study in collaboration with registered dietitians to assure all nutritional requirements were met during the admission.One month following hospital discharge, patients will be contacted with a follow up questionnaire to assess if dietary behaviors have changed compared to baseline. Results: The study is in the enrollment phase. We plan to have preliminary data to present at the conference. Conclusions: A prior inpatient study illustrated that inpatient diet could impact dietary behaviors. We hypothesize that an intervention with either a PBD or MD in patients hospitalized for cardiovascular disease will have a positive impact on dietary behaviors one month post discharge.

The investigation of intertextuality within a corpus of Latin poetry is the main objective of the research functions of the so-called ‘Musisque Deoque Galaxy’: formular recurrences, and lexical and metric-verbal co-occurrences draw a dense network of relationships between texts, where poetic memory presents itself in various modalities. This contribution aims to analyse the latest developments in the “Musisque Deoque Galaxy” both from the point of view of the tools created to enable new textual acquisitions and new analyses, and from the technological point of view, with reference to the work carried out to ensure a long-term sustainability.

This work reports on the electrochemical behaviour of Fe and Zn based metal-organic framework (MOF) compounds, which are “doped” with chiral molecules, namely: cysteine and camphor sulfonic acid. Their electrochemical behaviour was thoroughly investigated via “solid-state” electrochemical measurements, exploiting an “ad hoc” tailored experimental set-up: a paste obtained by carefully mixing the MOF with graphite powder is deposited on a glassy carbon (GC) surface. The latter serves as the working electrode (WE) in cyclic voltammetry (CV) measurements. Infrared (IR), X-ray diffraction (XRD) and absorbance (UV-Vis) techniques are exploited for a further characterization of the MOFs’ structural and electronic properties. The experimental results are then compared with DFT based quantum mechanical calculations. The electronic and structural properties of the MOFs synthesized in this study depend mainly on the type of metal center, and to a minor extent on the chemical nature of the dopant.