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Orange juice is considered a rich source of carotenoids, which are thought to have diverse biological functions. In recent years, a fermentation process has been carried out in fruits resulting in products that provide higher concentrations of bioactive compounds than their original substrates. The aim of this study was to evaluate the effect of a controlled alcoholic fermentation process (15 days) on the carotenoid composition of orange juice. Twenty-two carotenoids were identified in samples. The carotenoid profile was not modified as result of the fermentation. Total carotenoid content and provitamin A value significantly increased from day 0 (5.37 mg/L and 75.32 RAEs/L, respectively) until day 15 (6.65 mg/L and 90.57 RAEs/L, respectively), probably due to a better extractability of the carotenoids from the food matrix as a result of processing. Therefore, the novel beverage produced could provide a rich source of carotenoids and exert healthy effects similar to those of orange juice. © 2014 American Chemical Society., This study was supported by funding from the Ministerio de Ciencia e Innovación (Spanish government, Project AGL2010-14850/ALI) and the Consejerı́a de Economı́a, Innovación, Ciencia y Empleo (Junta de Andalucı́a, Projects P08-AGR-03477 and P09-AGR4814, and Grupo PAI BIO311). The Research Project grant of B.E.-L. is supported by the Junta de Andalucı́a. D.H.-M. is member of the IBERCAROT Network 112RT0445 financed by CYTED.

The use of persimmon (Diospyros kaki L.f.) as a possible functional food material has not garnered widespread popularity. We conducted the present study and found that a fat-soluble extract obtained from the peel contained a large amount of carotenoids; beta-cryptoxanthin accounted for more than 50% of the total carotenoids identified. The extract also contained 6 polyphenolic aglycons.

Beta-cryptoxanthin (betaCx), one of 4 major carotenoids in the blood, was investigated for anticarcinogenic activity in F344 rats. Four groups of 25 rats each received an intrarectal dose of 2 mg of N-methylnitrosourea 3 times a week for 5 weeks, and were fed the diet supplemented with 0 ppm (control), 25 ppm, 5 ppm or 1 ppm betaCx throughout the experiment. The colon cancer incidence at week 30 was significantly lower in the betaCx (25 ppm) diet group, but not in the betaCx (5 ppm) and betaCx (1 ppm) diet groups, than in the control diet group: 68%, 84%, 80% vs. 96%. The results suggested that dietary betaCx may affect colon carcinogenesis after accumulation in the colonic mucosa, perhaps due to absorption from the colon as well as the small intestine.

The effect of the industrial processing of peppers for paprika on carotenoids with provitamin A activity has been investigated. The processing consists of two principal steps: drying and milling. The milling step appears to be the more destructive, leading to a loss of 42.7 to 55.2% in the total carotenoid content and 65.2 to 81.4% in that of provitamin A, depending on the variety of the fruit. However, adding seeds from the peppers during this step dilutes the paprika, reducing the carotenoid losses due to the process itself to around 20%. The drying step is less destructive, but its final effect depends markedly on the degree of ripeness of the fruit and the variety of peppers used. Carotenoids with provitamin A value present in Capsicum annuum are β-carotene, β-cryptoxanthin, and cryptocapsin, which are degraded during the process by 67.3 to 82.2%, 59.2 to 78.9%, and 54.1 to 58.1 % respectively, resulting in a loss of provitamin A activity of ca. 65.2 to 81.4%.

β-Cryptoxanthin (1) was acetylated and then epoxidized with monoperoxyphthalic acid. After hydrolysis, repeated chromatography, and crystallization, (3S,5R,6S)-5,6-epoxy-β-cryptoxanthin (3), (3S,5S,6R)-5,6-epoxy-β-cryptoxanthin (4), (3R,5′R,6′R)-5′,6′-epoxy-β-cryptoxanthin (5), (3S,5R,6S,5′R,6′S)-5,6:5′,6′-diepoxy-β-cryp-toxanthin (6), and (3S,5S,6R,5′S,6′R)-5,6:5′,6′-diepoxy-β-cryptoxanthin (7) were isolated as main products and characterized by their UV/VIS, CD, 1H- and 13C-NMR, and mass spectra. The comparison of the carotenoid isolated from yellow, tomato-shaped paprika (Capsicum annuum var. lycopersiciforme flavum) with 3–5 strongly supports the structure of 3 for the natural product.

Liposomes, in which beta-carotene, beta-cryptoxanthin, zeaxanthin, beta-cryptoxanthin palmitate or beta-cryptoxanthin acetate had been embedded, were irradiated by UVA, and the rate of degradation of each carotenoid was measured. There was no significant difference in the degradation rate between beta-carotene, beta-cryptoxanthin and zeaxanthin. The degradation rates of beta-cryptoxanthin palmitate and beta-cryptoxanthin acetate were faster than that of beta-cryptoxanthin, and the degradation rate of beta-cryptoxanthin palmitate was faster than that of beta-cryptoxanthin acetate.

The role of vitamin A in bone health remains controversial. Some human studies associate higher concentrations of specific carotenoids with improved bone health.

Degreening pada buah jeruk tropika dataran rendah seringkali gagal menghasilkan buah jeruk berwarna jingga, tetapi kuning. Warna jingga terbentuk dari campuran dua pigmen kulit jeruk, yaitu β-cryptoxanthin (kuning) dan ß-citraurin (kuning kemerahan). ß-citraurin sering kali tidak terbentuk pada buah jeruk tropika dataran rendah karena pembentukannya terjadi apabila buah terpapar suhu rendah saat pertumbuhannya. Precooling buah jeruk tropika dataran rendah diharapkan dapat mendorong pembentukan ß-citraurin karena jeruk yang sudah dipanen masih melakukan metabolisme. Konsentrasi etilen adalah salah satu faktor pembatas keberhasilan degradasi klorofil dalam degreening. Penelitian ini bertujuan untuk mengetahui pengaruh precooling sebelum degreening dan konsentrasi etilen terbaik pada degreening buah jeruk siam. Buah dipetik di kebun jeruk rakyat di Jember dan perlakuan degreening dilaksanakan di Laboratorium Pascapanen, Pusat Kajian Hortikultura Tropika, Institut Pertanian Bogor pada bulan Februari 2014. Penelitian menggunakan rancangan acak kelompok dua faktor. Faktor pertama adalah perlakuan precooling (dengan precooling dan tanpa precooling). Faktor kedua adalah konsentrasi etilen (0, 100, 200, 300 ppm). Perlakuan degreening buah jeruk siam asal Jember yang diawali dengan perlakuan precooling pada suhu 5oC menghasilkan jeruk berwarna jingga, sedangkan yang tanpa precooling menghasilkan warna kuning. Etilen 100 ppm adalah konsentrasi terbaik dalam degradasi klorofil. Perlakuan precooling dan degreening tidak berpengaruh buruk pada kandungan asam dan gula buah jeruk.

Background Serum β-cryptoxanthin levels are lower in overweight subjects than in normal subjects. Abnormalities of adipocytokine profiles in obesity subjects have been reported. There are several reports that serum β-cryptoxanthin levels in them were relatively lower than normal subjects. Objective We hypothesize that supplementation of highly concentrated β-cryptoxanthin improves serum adipocytokine profiles in obese subjects. This study tested the association between β-cryptoxanthin intake and serum adipocytokine levels. Methods An intervention study consisted of a 3-week long before-and-after controlled trial, where β-cryptoxanthin (4.7 mg/day) was given to 17 moderately obese postmenopausal women. Results The results indicated no significant changes in body weight or body mass index (BMI). Serum β-cryptoxanthin levels increased significantly by 4-fold. Serum high molecular weight (HMW)-adiponectin levels increased significantly, while serum plasminogen activator inhibitor (PAI)-1 levels decreased. Conclusions We concluded that increasing the intake of β-cryptoxanthin to approximately 4 mg per day for 3 weeks may have beneficial effects on the serum adipocytokine status and consequently alleviate progression of metabolic syndrome.

Human serum and tissues contain in excess of 12 dietary carotenoids and several metabolites that originate from consumption of fruits and vegetables. Among these are hydroxycarotenoids: (3R,3'R,6'R)-lutein (1), (3R,3'R)-zeaxanthin (2), (3R,6'R)-α-cryptoxanthin (3), and (3R)-β-cryptoxanthin (4). In addition, several dehydration products of 1 have also been identified in human serum, these are: (3R,6'R)-3-hydroxy-3',4'-didehydro-β,γ-carotene (5), (3R,6'R)-3-hydroxy-2',3'-didehydro-β,ε-carotene (6), and (3R)-3-hydroxy-3',4'-didehydro-β,β-carotene (7). Several metabolites of 1 and/or 2, namely, (3R,3'S,6'R)-lutein (3'-epilutein, 8) and (3R,3'S;meso)-zeaxanthin (9) have also been characterized in human serum and ocular tissues. Semi-synthetic processes have been developed that separately transform commercially available 1 into 4 via 7 as well as 1 into 8. While 8 is converted into 2 by base-catalyzed isomerization, 7 is transformed into 2 and its (3R,3'S;meso)-stereoisomer (9) by regioselective hydroboration.

The laying hen has a natural ability to deposit carotenoids into its egg yolks, especially the xanthophyll carotenoid lutein that is used commercially as an egg colorant. Can this ability to deposit carotenoids be used to enrich egg yolk provitamin A value? After a 10-d carotenoid depletion period in hens (n = 24), the effects of a 20-d intervention with high-β-cryptoxanthin, high-β-carotene, or typical yellow maize on color and carotenoid profile were compared with the effects of a white maize diet (n = 6/treatment). Eggs were collected every other day and yolks were analyzed by using a portable colorimeter to define the color space and by using an HPLC to determine the carotenoid profile. The high-β-cryptoxanthin and yellow maize increased β-cryptoxanthin in the yolk (0.55 ± 0.08 to 4.20 ± 0.56 nmol/g and 0.55 ± 0.08 to 1.06 ± 0.12 nmol/g, respectively; P < 0.001). Provitamin A equivalents increased in eggs from hens fed high-β-cryptoxanthin maize (P < 0.001) but not the high-β-carotene maize. The color (L*, a*, and b*) assessment of the yolks showed an increase in the high-β-cryptoxanthin treatment for the red-green a* scale (P < 0.001) and a decrease for the light-dark L* scale (P < 0.001). No appreciable change was noted in the yellow-blue b* scale for the high-β-cryptoxanthin treatment; but significant changes were noted for the yellow (P = 0.002) and high-β-carotene maize (P = 0.005) treatments, which were most evident at the end of the washout period with white maize. β-Cryptoxanthin-biofortified maize is a potential vehicle to elevate provitamin A equivalents and to enhance the color of yolks. This could lead to a human health benefit if widely adopted.

β-Cryptoxanthin (β-cry), an antioxidant abundant in citrus fruits, plays an important role in the prevention and treatment of certain diseases, especially cancers. In the present study, to increase the content of β-cry in citrus flavedo, the effects of blue (470 nm) and red (660 nm) light-emitting diode (LED) lights on the accumulation of carotenoids and expression of genes related to carotenoid biosynthesis were investigated in the flavedo of Satsuma mandarin. The results showed that accumulation of β-cry was induced by red light, while it was not affected by blue light. The accumulation of β-cry under red light was attributed to simultaneous increases in the expression of CitPSY, CitPDS, CitZDS, CitLCYb1, CitLCYb2, CitHYb, and CitZEP. The results presented herein might provide new strategies to enhance the commercial and nutritional value of citrus fruits.

Summary: β-Cryptoxanthin displays a unique anabolic effect on bone calcification. In women with osteoporosis, serum β-cryptoxanthin and 25-OH-vitamin D3 showed a weak but significant correlation and exhibited a complementary seasonal distribution. The potential role of β-cryptoxanthin as a nutritional approach to improving bone health deserves further evaluation. Introduction: Dietary intake and serum levels of β-cryptoxanthin have been inversely related to different bone and joint disorders and in vitro and animal studies have shown that β-cryptoxanthin displays a unique anabolic effect on bone calcification. Due to the emerging role of β-cryptoxanthin in bone biology, we aimed to assess the serum distribution and variability of β-cryptoxanthin and their potential relation to 25-OH-vitamin D3 in women with osteoporosis. Methods: Serum concentrations of α- and β-cryptoxanthin and 25-OH- D 3 in women with osteoporosis (N=644) were analyzed using a quality-controlled high-performance liquid chromatography (HPLC) method. Results: Overall, significant seasonal variations were found for the three analytes and inter-individual variation was also high (60-73%). β-cryptoxanthin and 25-OH-vitamin D3 exhibited a marked complementary seasonal distribution in serum, with vitamin D displaying the highest values in summer and β-cryptoxanthin in winter. Conclusions: Given the anabolic effect of β-cryptoxanthin on bone calcification and its complementary seasonal distribution with respect to 25-OH-vitamin D3, the potential role of β-cryptoxanthin as a sustainable nutritional approach to improving bone health deserves to be further evaluated. © 2007 International Osteoporosis Foundation and National Osteoporosis Foundation., β-Cryptoxanthin . Bone health . human study . Osteoporosis. 25-OH-vitamin D3

β-Cryptoxanthin monoepoxide has been widely found in food, but the position of the epoxy group has not been confirmed. In terms of nutrition, this confirmation is important because, depending on the location of the epoxy substituent, this carotenoid may or may not be a provitamin A. In papaya cultivar Formosa it is present in amounts high enough to affect the vitamin A value substantially. Mass spectrometry and 1H-NMR spectroscopy have unequivocally proved the structure to be 5,6-epoxy-5,6-dihydro-β,β-caroten-3-ol; thus, it is vitamin A active.

Few studies provide correlations between different indicators of the dietary intakes of older (>or= 65 y) black and white adults. This study compared the usual intakes of vitamin E, vitamin C, and selected carotenoids estimated by a modified Harvard food frequency questionnaire (FFQ) to those estimated by multiple 24-h recalls, and to blood concentrations of components in a randomly selected sample of participants in the Chicago Health and Aging Project (CHAP). Subjects (n = 59) were interviewed to complete multiple 24-h recalls over a year's time, then completed an FFQ and subsequently provided a fasting blood specimen within 2 mo. Dietary estimates were energy-adjusted separately for men and women. Significant (P < 0.05) correlations between total (diet and supplement) FFQ estimates and serum measures of vitamin E and vitamin C were as follows: 0.49 and 0.39 for blacks, and 0.42 and 0.29 for whites, respectively. The highest correlations between serum and FFQ indicators were for dietary beta-cryptoxanthin (0.46), total alpha-tocopherol (0.46) and total beta-carotene (0.44) among whites; among blacks, the highest correlations were for dietary alpha-carotene (0.81), total alpha-tocopherol (0.53) and total beta-cryptoxanthin (0.50); all were significant (P < 0.05). Further adjustment for age, gender, BMI, and educational level minimally altered these coefficients. These findings indicate that the modified Harvard FFQ provides reasonable estimates of serum levels of vitamin E, vitamin C and beta-cryptoxanthin among CHAP participants.

(±)-3-Hydroxy-β-ionone has been prepared and converted into zeaxanthin, β-cryptoxanthin, and zeinoxanthin (α-cryptoxanthin).

Pandanus conoideus is an endemic plant of Papua, Indonesia, reported to be very rich in carotenoids. The purpose of this study was to develop method for the determination of carotenoids (α-cryptoxanthin, β-cryptoxanthin, α-carotene and β-carotene) in P. conoideus oil (PO) by high-performance liquid chromatography (HPLC). sing the proposed method in this research, carotenoids content of nine clones of PO were analyzed which ranged from 5.4-138.5 ng/mg for α-cryptoxanthin, 3.9-29.4 ng/mg for β-cryptoxanthin, 3.5-80.0 ng/mg for α-carotene, and 10.8-118.0 ng/mg for β-carotene. Our results showed that four carotenoids content was very small as compared to total carotenoids content (3027-19959 ng/mg). This suggests that those four carotenoids were not a major component of the PO carotenoids. Using the principal component analysis, nine clones of P. conoideus can be grouped based on the proximity of its carotenoid content into group A ( Monsor, Mbarugum, Himbiak, Monsrus and Memeri ), group B ( Menjib Rumbai ), and group C ( Edewewits, Hibcau and Hityom ).