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To investigate the possibility of using bamboo-bundle laminated veneer lumber (BLVL) as a cooling tower packing material, the water absorption rates, thickness swelling rates, and flexural properties of three different composite materials were studied. The BLVL was combined with either 12% or 24% phenol formaldehyde resin (PF), and the moso bamboo strips were exposed to water baths at three different temperatures (45, 65, and 85 °C) for 30 d. After the aging treatments, the 24%-BLVL samples showed lower water absorption rates and better bending properties than the other two composites. The temperature was found to have a significant effect on the modulus of rupture (MOR), modulus of elasticity (MOE), and the thickness swelling rate. As the temperature increased, the swelling rate and the rate of weight gain increased and the MOE and MOR decreased. According to the activation energies for swelling calculated from the Arrhenius-type plots, compared with the 24%-BLVL (22.95 kJ·mol-1) and the moso bamboo strips (12.69 kJ·mol-1), the effect of temperature on the swelling rate was greatest for the 12%-BLVL (24.15 kJ·mol-1). Results showed that the BLVL material is a promising candidate for a novel cooling tower packing material.

Tensile properties of bamboo fiber (BF)/polypropylene (PP) composites were investigated for different impregnation modification processes. The surface morphology of BF and the fracture morphology of the BF/PP composites were examined by scanning electron microscopy (SEM). Images showed that CaCO3 nanoparticles (nano-CaCO3) adhered to the fiber surfaces. Tension tests of individual bamboo fibers (IBFs) and the BF/PP composites were conducted. The results indicated that the tensile strength (TS), modulus of elasticity (MOE), and elongation at break (EAB) of IBFs increased by 16.0%, 22.2%, and 5.2%, respectively, while those of the BF/PP composites increased by 3.0%, 7.0%, and 15.8%, respectively, compared to the control samples. Various process parameters during the impregnation with nano-CaCO3 had marked effects on the tensile properties of the composites. The optimal conditions for BF-reinforced PP composites by impregnation modification were determined to be a nano-CaCO3 concentration of 1.0×10-2 g/mL, an ethylenediamine tetraacetic acid disodium salt (EDTA-2Na) additive concentration of 8.5×10-4 g/mL, and an impregnation time of 25 min.

The purpose of this study was to investigate the effect of white mud (WM) on mechanical and thermal properties of bamboo plastic composites (BPCs). Bamboo residue fibers (BRFs) and WM were added as the reinforcement, and high-density polyethylene (HDPE) served as the matrix. The lubricating agent and coupling agent were polyethylene wax and maleated polyethylene (MAPE), respectively. The mixture was used to manufacture BPCs using a twin-screw extruder. The crystal structure and thermal properties of BPCs were investigated by X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The results showed that the particle size of the WM was 700 nm to 50 μm, which are made mainly of calcium carbonate. BPCs with WM significantly increased the flexural and tensile properties, but the impact strength decreased because of the presence of WM. The flexural and tensile strength of composites with 18 wt% BPF were increased by 36.81% and 6.26%, respectively, while the flexural and tensile modulus were increased by 164.29% and 64.33%, respectively. XRD demonstrated the WM had little effect on the crystal structure of BPCs. Compared to BPCs without WM, the T5% of composites with 22 wt% WM decreased by 27.9 °C. As the WM content increased, the crystallinity of the BPCs decreased initially, then increased with increasing WM content.

Bamboo-bundle laminated veneer lumber (BLVL) was produced by joint lengthening technology. The objective of this study was to evaluate the effect of joint form and the values of key parameters on mechanical properties of BLVL. Two joint forms, i.e., finger joint and scarf joint, and two corresponding joint parameters, i.e., finger length and scarf angle, were investigated in laminates. The results indicated that the mechanical properties of jointed BLVL were reduced in comparison to those of BLVL without joints. For finger-joint form, the BLVL member with 14 mm fingers achieved better compressive strength, bending modulus of rupture (MOR), and tensile strength than 19 and 25 mm ones. For scarf-joint form, the 30° scarf-jointed member exhibited the highest bending modulus and tensile modulus of rupture (MOR) in this study, then followed the 45° and 60° ones. The 60° scarf-jointed BLVLs were better than 30° and 45° groups in compression strength. Synthesis of the testing results revealed that finger-jointed BLVLs achieved better bending modulus of rupture than scarf-jointed ones.

To predict the resin absorbing content of bamboo bundle fiber, a model relating to the soaking time and mass ratio of polyvinyl acetate (PVAC) adhesive to phenol formaldehyde (PF) resin defined on the basis of the Lucas-Washburn equation was proposed. Various dipping times and different PVAC/PF ratios were investigated in the experiment. Variance analysis revealed that the resin absorbing content was significantly associated with the ratio of PVAC/PF. However, the soaking time had a significant effect on bamboo fiber resin absorbing content from 0 s to 70 s and had an insignificant effect after 70 s. The results showed that the model proposed could accurately predict the resin absorbing content for bamboo bundle fiber impregnated in different PVAC/PF levels. The comparison of theoretical calculations with experimental results revealed that the residual sum of square and root mean squared error were relatively low, and the correlation coefficients and coefficients of determination were all over 0.95.

The purpose of this study was to investigate the mechanical and thermal properties of high-density polyethylene (HDPE) composites reinforced by bamboo pulp fibers (BPF). Using a twin-screw extruder, polymer composites were fabricated using BPF and bamboo flour (BF) as the reinforcement and HDPE as the matrix. Tensile and flexural tests of the HDPE composites were performed to determine the mechanical properties under different conditions. The thermal properties of HDPE composites were characterized by thermogravimetric analysis (TGA) and dynamic mechanical analysis (DMA). The results showed that BPF improved the mechanical and thermal properties of the polymer composites more than did BF. The tensile and flexural strength of composites with 30 wt% BPF were increased by 61.46% and 22.94%, respectively, while the tensile and flexural modulus were increased by 84.52% and 27.30%, respectively. Compared to composites with 50 wt% BF, the T5% of composites with 50 wt% BPF increased by 20.18 °C. As the BPF content increased, the storage modulus (E’) and loss modulus (E”) initially increased, followed by a decrease. Compared to the BF/HDPE composites, BPF/HDPE composites reinforced at 30 wt% had a higher storage modulus (E’) and loss modulus (E”) and lower damping parameter (tanδ).

This paper introduces the creative process of making a digital animated film from the ancient murals of Mogao Cave 254 at Dunhuang, Gansu Province, showing the artistic value and touching spiritual connotations of the murals. [ABSTRACT FROM AUTHOR]

The demand for eco-friendly renewable natural fibers has grown in recent years. In this study, a series of polypropylene-based composites reinforced with single bamboo fibers (SBFs), prepared by non-woven paving and a hot-pressing process, were investigated. The influence of the content of SBF on impact strength, flexural strength, and water resistance was analyzed. The properties of the composites were greatly affected by the SBF content. Impact strength increased as SBF content increased. The modulus of rupture and modulus of elasticity show an optimum value, with SBF contents of 40% and 50%, respectively. The surface morphology of the fractured surfaces of the composites was characterized by scanning electron microscopy. The composites showed poor interfacial compatibility. The water resistance indicates that the composites with higher SBF contents have higher values of water absorption and thickness swelling, due to the hydrophilicity of the bamboo fibers. The thermal properties of the composites were characterized by thermal gravimetric analysis and by differential scanning calorimetry. The thermal stability of the composites was gradually reduced, due to the poor thermal stability of SBFs. In the composites, the maximum decomposition temperature corresponding to SBF shows an increasing trend. However, the maximum decomposition temperature of polypropylene was not influenced by SBF content. The melting point of the polypropylene in the composites was lower relative to pure polypropylene, although it was not affected by increasing SBF content.

The focus of this report was to understand the tensile properties and dynamic mechanical properties of bamboo powder (BP) reinforced polylactic acid (PLA) composite filaments which were treated with nano calcium carbonate (CaCO3), cellulose nanofibers (CNF), and micro-crystalline cellulose (MCC) using impregnation modification technology. The storage modulus (E’) of nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments increased compared with BP/PLA composite filaments before the glass transition temperature Tg. When the temperature was above Tg, the reinforcement effect of nano CaCO3, MCC, and CNF gradually became less apparent. The loss modulus (E’’) and loss factor (tan δmax) of the nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments was higher than that of BP/PLA composite filaments produced by the “one-step” method. The tensile strength (TS) results showed a similar trend. Compared with the control samples, the TS of nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments produced by the “one-step” method (and the “two-step” method) increased by 40.33% (and 10.10%), 32.35% (and −8.61%), and 12.32% (and −12.85%), respectively. The TS of nano CaCO3-BP/PLA, MCC-BP/PLA, and CNF-BP/PLA composite filaments produced by the “one-step” method was slightly higher than those produced by the “two-step” method. The elongation at break (EAB) of BP/PLA composite filaments was higher than that of BP/PLA samples treated with nano CaCO3, MCC, or CNF. The PLA and modified BP were readily accessible through a simple mixing process. The rheological investigation of such mixtures showed that nano CaCO3, CNF, and MCC have different effects on the processability and rheological properties of composites.

In order to optimize the design of high-altitude sprinkling system of fire-fighting aircraft, it is necessary to establish a model reflecting the high-altitude spraying and distribution mechanism of mass fire extinguishing bags during the flight. Based on the discrete element method and the computational fluid dynamics method, a high-altitude spraying and distributing model of aircraft mass fire extinguishing bags is established, and the spraying characteristics and landing area distribution at different flight speeds and altitudes are obtained. The model is verified by experiments. The deviations between simulated landing distribution and experimental data are less than 20.0%. The research results provide a theoretical model for the development of aircraft high altitude fire extinguishing system, so as to significantly improve the fire extinguishing performance of aircraft spraying system.

The focus of this study was to observe the mechanical properties of bamboo plastic composites (BPCs) with bamboo pulp fiber (BPF) or white mud (WM). The essential work of fracture (EWF) methodology was used to characterize the impact toughness of BPCs. The results revealed an increase in flexural, tensile and impact properties, when adding the BPF in the BPCs. While the flexural properties of WM-reinforced BPCs revealed increasing, there was a decrease in tensile and impact strength. In an impact strength analysis study, BPF-filled BPCs showed excellent impact property over WM-filled BPCs; scanning electron microscopy (SEM) helps to explain impact fracture behavior of BPCs. EWF analysis of impact results showed that the specific essential work of fracture (we) increased significantly with the amount of BPF used in BPCs but decreased with the increase of WM in the BPCs. There was similar variation for the non-essential plastic work (βwp) of BPCs. This result indicates that the fracture initiation and fracture propagation of BPCs are different.

A new and eco-friendly mussel-inspired surface modification pathway for bamboo fiber (BF) is presented in this study. The self-assembly polydopamine (PDA) coating can firmly adhere on BF surface, which also serves as a bridge to graft octadecylamine (ODA) for hydrophobic surface preparation. The as-formed PDA/ODA hybrid layer could supply abundant hydrophobic long-chain alkyls groups and generated a marked increase in BF surface roughness and a marked decrease in surface free energy. These changes provided advantages to improve fiber–matrix interfacial adhesion and wettability. Consequently, high performance was achieved by incorporating the hybrid modified BF into the polybutylene succinate (PBS) matrix. The resultant composite exhibited excellent mechanical properties, particularly tensile strength, which markedly increased by 77.2%. Meanwhile, considerable high water resistance with an absorption rate as low as 5.63% was also achieved. The gratifying macro-performance was primarily attributed to the excellent interfacial adhesion attained by hydrogen bonding and physical intertwining between the PDA/ODA coating on the BF and the PBS matrix, which was further determined by fracture morphology observations and dynamic mechanical analysis. Owing to the superior adhesive capacity of PDA, this mussel-inspired surface modification method may result in wide-ranging applications in polymer composites and be adapted to all natural fibers.

A novel method has been developed to fabricate submicron beams with galvanic etch stop for Si in TMAH. The different Au:Si area ratios before and after the release of the beams are used to trigger the galvanic etch stop to fabricate submicron single crystal Si beams in standard Si wafers. Before the beams are released from the substrate, the Au electrodes are connected to the substrate electrically. The Au:Si area ratios are much smaller than the threshold value. TMAH etches the Si wafers. After the beams are fully released, they are mechanically supported by the Au wires, which also serve as the galvanic etch stop cathodes. The Au:Si area ratios are much larger than the threshold value. The beams are protected by galvanic etch stop. The thicknesses of the beams are determined by shallow dry etching before TMAH etching. A 530 nm thick beam was fabricated in standard (111) wafers. Experiments showed that the beam thicknesses did not change with over etching, even if the SiO2 layers on the surface of the beams were stripped.

The solvent-assisted flavor evaporation, sensory evaluation, and partial least squares regression analysis were used to screen the relatively better flavor of pork broth among different stewing time (1, 2, 4, 6, and 8 h). A total of 48 volatile compounds were successfully characterized by gas chromatographymass spectrometry in the pork broth, which stewed for 4 h. The dominant volatiles were confirmed by aroma extract dilution analysis. Twenty-seven odorants with flavor dilution factors between 2 and 1,024 were identified. Among them, odor activity values of 19 components were greater than or equal to 1. An aroma recombination test was performed, and a similar flavor (93.04 %) was simulated. Omission test further confirmed that 4-hydroxy-2,5-dimethyl-3(2H)-furanone, hexanal, 1-octen-3-ol, (E)-2-octenal, (E)-2-decenal, (E)-2-undecanal, (E, E)-2,4-decadienal, nonanoic acid, decanoic acid, 2-heptanone, 3-hydroxy-2-butanone, δ-decanolactone, and 2-acetylpyrrole were the key odorants of the aroma profile of pork broth. Practical Application: Pork broth is popular in China, but lacks the study of its key aroma compounds, which restricts its industrial production. This study researched the optimum stewing time of pork broth and analyzed its key aroma compounds. Finally, the flavor profile can be obtained and understood. This study could provide a reference and further promote research on pork flavor. [ABSTRACT FROM AUTHOR]

The focus of this study was to observe the effect of nano calcium carbonate (CaCO3) modification methods on bamboo fiber (BF) used in BF-reinforced high-density polyethylene (HDPE) composites manufactured by extrusion molding. Two methods were used to introduce the nano CaCO3 into the BF for modification; the first was blending modification (BM) and the second was impregnation modification (IM). In order to determine the effects of the modification methods, the water absorption, surface free energy and interfacial properties of the unmodified composites were compared to those of the composites made from the two modification methods. The results revealed that the percentage increase in the weight of the composite treated by nano CaCO3 decreased and that of the IMBF/HDPE composite was the lowest over the seven months of time. The results obtained by the acid-base model according to the Lewis and Owens-Wendt- Rabel-Kaelble (OWRK) equations indicated that the surface energy of the composites was between 40 and 50 mJ/m2. When compared to the control sample, the maximum storage modulus (E′max) of the BMBF/HDPE and IMBF/HDPE composites increased 1.43- and 1.53-fold, respectively. The values of the phase-to-phase interaction parameter B and the k value of the modified composites were higher than those of the unmodified composites, while the apparent activation energy Ea and interface parameter A were lower in the modified composites. It can be concluded that nano CaCO3 had an effect on the interfacial properties of BF-reinforced HDPE composites, and the interface bonding between IMBF and HDPE was greatest among the composites.

To effectively obtain the downforce of the gauge wheels in real time, mechanical models of the interaction among the ground, gauge wheels, gauge wheel arms, and depth adjustment lever were constructed. A measuring method was proposed for monitoring the downforce through a two-dimensional radial sensing device, and a corresponding prototype was designed. Through simulation analysis of the sensing device with ANSYS, a 45° angle was determined to exist between the strain gauge axis and the sensing device axis, and the Wheatstone bridging circuit of R1+R3-R5-R7 (R stands for resistance strain gauge, different figures represent the strain gauge number) and R2+R4-R6-R8 was adopted. According to performance and calibration tests for the sensing device, the maximum interaction effect between the X and Y axes was 2.52%, and the output signal was stable and consistent. The standard error of the slope of the fitting equation of the downforce calculation model is 0.008. According to the field test, the average downforce of the gauge wheels was 1148, 1017, 843, and 713 N, at different sowing speeds of 6, 8, 10, and 12 km/h, respectively. The coefficients of variation were 0.40, 0.41, 0.62, and 0.71, respectively. The results indicate that the downforce fluctuation of the gauge wheels became more severe with increasing planting speed. Both the strain simulation analysis and field test verified that the measurement method is accurate and reliable, the performance of the sensing device is stable, the measurement method and sensing device meet the application requirements and lay a foundation for the research of accurate and stable control of downforce of no-till planter. [ABSTRACT FROM AUTHOR]

BackgroundThe health impairment caused by ovarian cancer in female population has become a global focus. However, there is a lack of research on ovarian cancer incidence and mortality trends in Chinese female population.ObjectiveTo describe the trends of ovarian cancer incidence and mortality in China from 2005 to 2016 by analyzing the incidence and death data of ovarian cancer collected from Chinese Cancer Registry Annual Report, providing a basis for the scientific prevention and control of ovarian cancer in China.MethodsThe incidence and mortality of ovarian cancer in China during 2005 to 2016 were collected and input into Excel spreadsheets, then the annual age-standardized incidence rate, age-standardized mortality rate, age-specific incidence rate and age-specific mortality rate were computed. Joinpoint Regression Program was used to estimate the annual percentage change (APC) , and average annual percentage change (AAPC) to analyze the temporal trends of annual ovarian cancer incidence and mortality rates.ResultsThe incidence rate of ovarian cancer showed a trend of rapid increase over the period of 2005 to 2016 (AAPC=7.25%, P

Based on the data of the two water releasing tests and “5.25” water inrush of collapse columns of Pan’er Coal Mine in Huainan Coalfield, the hydraulic connection between the main aquifers and transmissivity-impermeability of faults were systematically analyzed by means of groundwater system analysis, structural water control theory and statistical analysis. The results show that: there a close hydraulic connection between Ordovician limestone aquifers with C3III group limestone aquifers of Taiyuan Formation with the same water level response and variation range. Besides, the hydraulic connection between Ordovician limestone aquifer with C3I and C3II group limestone aquifer of Taiyuan Formation is weak. F1 fault is impermeability in C3I and C3II group limestone aquifer of Taiyuan Formation but transmissivity in C3III group limestone aquifers of Taiyuan Formation and Ordovician limestone aquifers. In addition, DF1 fault is impermeability in C3I group limestone aquifer of Taiyuan Formation but transmissivity in C3II, C3III group limestone aquifers of Taiyuan Formation and Ordovician limestone aquifers.

In this paper the antiplane harmonic dynamics stress of an infinite isotropic wedge with a circular cavity is analyzed for the first time by using a novel method with Green's function, complex functions, and multipolar coordinates. A basic solution for the displacement field of an elastic half-space containing a circular cavity subjected to anti- plane harmonic point force is employed as the Green's function. Based on the Green's function, the infinite wedge problem is equivalently transformed into the problem of a half-space divided by a semi-infinite traction free line. The equivalent problem is solved numerically to determine the dynamic stress field in the wedge at different apex angles and cavity locations. We show that the wedge angle, cavity location, and incident angle and frequency of the external load have significant effect on the dynamic stress of the cavity surface. The dynamic stress concentration factor on the cavity surface becomes singular when the cavity is close to the boundary of the wedge. [ABSTRACT FROM AUTHOR]

Soil penetration resistance (SPR) is one of the major indicators of soil physical properties. Crop residue managements, tillage practices and their interactions exert significant effects on the SPR. However, rare information is available in the sloping farmland of Mollisols. Field experiments were conducted to investigate the variations of the SPR as affected by crop residue managements and tillage practices on the sloping land in Northeast China from 2015 to 2019. The split-plot experiment was arranged with two crop residue managements (removed, REM and retained, RET), and three tillage practices (no tillage, NT; rotary tillage, RT; plow tillage, PT). SPR data in 0-80 cm soil depth was measured at the end of harvest of maize monoculture. Results showed that the two crop residue managements induced significant variations in the SPR at 0-15 cm, 15-30 cm and 0-80 cm soil depths under NT, RT, and PT treatments, respectively. In comparison with RET treatment, the average values of the SPR under REM treatment were 10.9% and 8.9% higher in 45-60 cm and 60-80 cm soil depths, respectively. The average values of the SPR under PT treatment were 12.4% and 14.1% lower in 0-15 cm soil depth, and 23.9% and 10.4% lower in 15-30 cm soil depth than those under NT and RT treatments. However, the average value of the SPR under PT treatment was 11.2% and 22.0% higher in 60-80 cm soil depth than those under NT and RT treatments, respectively. The SPR generally decreased with the slope position declined in the deeper soil depth (except for the NT+RET treatment). The findings from this research can provide a scientific reference for the establishment of rational cultivation and the sustainable development of productivity on the sloping land of Mollisols in cold regions. [ABSTRACT FROM AUTHOR]

The viscoelastic behavior and the dynamic mechanical properties were studied for wood-melamine-urea-formaldehyde composites prepared from fast-growing poplars using in situ polymerization. The changes in stress relaxation (SR), storage modulus (E′), and damping parameter (tan δ) were considered. The relationship between the dynamic mechanical properties and the modifier concentration was also studied. The SR value decreased as the modifier content increased in all the samples examined. The urea content had a greater impact than the melamine-ureaformaldehyde mixture content on the SR value of the wood samples. A similar trend was observed for E′. The modified wood samples exhibited a higher tan δ. The results confirmed that a better interfacial interaction between melamine-urea-formaldehyde and wood fiber contributed to the improvement of mechanical properties in the fast-growing poplar wood. [ABSTRACT FROM AUTHOR]

Magnetotactic bacteria (MTB) are phylogenetically diverse prokaryotes that are able to biomineralize intracellular, magnetic chains of magnetite or greigite nanocrystals called magnetosomes. Simultaneous characterization of MTB phylogeny and biomineralization is crucial but challenging because most MTB are extremely difficult to culture. We identify a large rod, bean-like MTB (tentatively named WYHR-1) from freshwater sediments of Weiyang Lake, Xi'an, China, using a coupled fluorescence and scanning electron microscopy approach at the single-cell scale. Phylogenetic analysis of 16S rRNA gene sequences indicates that WYHR-1 is a novel genus from the Deltaproteobacteria class. Transmission electron microscope observations reveal that WYHR-1 cells contain tens of magnetite magnetosomes that are organized into a single chain bundle along the cell long axis. Mature WYHR-1 magnetosomes are bullet-shaped, straight and elongated along the [001] direction, with a large flat end terminated by a {100} face at the base and a conical top. This crystal morphology is distinctively different from bullet-shaped magnetosomes produced by other MTB in the Deltaproteobacteria class and the Nitrospirae phylum. This indicates that WYHR-1 may have a different crystal growth process and mechanism from other species, which results from species-specific magnetosome biomineralization in MTB. [ABSTRACT FROM AUTHOR]

Prostate cancer subtypes are poorly defined and functional validation of drivers of ETS rearrangement-negative prostate cancer has not been conducted. Here, we identified an ETS- subtype of aggressive prostate cancer (ERG-MAP3K7del CHDldel) and used a novel developmental model and a cell line xenograft model to show that cosuppression of MAP3K7 and CHD1 expression promotes aggressive disease. Analyses of publicly available prostate cancer datasets revealed that MAP3K7 and CHD1 were significantly codeleted in 10% to 20% of localized tumors and combined loss correlated with poor disease-free survival. To evaluate the functional impact of dual MAP3K7-CHD1 loss, we suppressed Map3k7 and/or Chd1 expression in mouse prostate epithelial progenitor/stem cells (PrP/SC) and performed tissue recombination experiments in vivo. Dual shMap3k7-shChd1 PrP/SC recombinants displayed massive glandular atypia with regions of prostatic intraepithelial neoplasia and carcinoma apparent. Combined Map3k7-Chd1 suppression greatly disrupted normal prostatic lineage differentiation; dual recombinants displayed significant androgen receptor loss, increased neuroendocrine differentiation, and increased neural differentiation. Clinical samples with dual MAP3K7-CHD1 loss also displayed neuroendocrine and neural characteristics. In addition, dual Map3k7-Chd1 suppression promoted E-cadherin loss and mucin production in recombinants. MAP3K7 and CHD1 protein loss also correlated with Gleason grade and E-cadherin loss in clinical samples. To further validate the phenotype observed in the PrP/SC model, we suppressed MAP3K7 and/or CHD1 expression in LNCaP prostate cancer cells. Dual shMAP3K7-shCHD1 LNCaP xeno-grafts displayed increased tumor growth and decreased survival compared with shControl, shMAP3K7, and shCHD1 xeno-grafts. Collectively, these data identify coordinate loss of MAP3K7 and CHD1 as a unique driver of aggressive prostate cancer development. [ABSTRACT FROM AUTHOR]

Backgroud: Malnutrition has been confirmed to play an important role in colorectal cancer (CRC) progression via the gut microenvironment. However, the characteristics of the gut microbiota or its potential biological mechanism in CRC remain inconclusive., Methods: In this work, Patient-Generated Subjective Global Assessment (PG-SGA) tool and 16sRNA sequencing were prepared to detect the variation in gut microbiota and the association between nutrition status and gut microbiota. RDA/CCA analysis was used to evaluate the relationship between faecal microbiota from malnourished CRC and clinical nutrition indicators. To investigate the mechanism of the gut microbiota in CRC, faecal samples from malnourished CRC patients were transplanted into C57BL/6J and DSS/AOM mouse models. Moreover, FACS and IHC were prepared to detect the infiltration of B cells and macrophages. qPCR and Elisa assays were performed to explore the expression of cytokines., Result: We found dramatic variation in the faecal microbiota among patients with different nutritional statuses, discovering that specific microbiota species, namely, Atopobium vaginae, Selenomonas sputigena and Faecalibacterium prausnitzii, may be considered diagnostic biomarkers in malnutrition and indicate poor prognosis. High expression level of A. vaginae in CRC tissues revealed the poorer overall survival compared with low expression level (Mean survival: 23.0 months vs 29.0 months). Faecal from malnourished colorectal cancer were found to be protumorigenic. More importantly, our evidence suggests that after faecal microbiota transplantation, B cells and macrophages are recruited to activate specific tumour immunity in CRC. Depletion of B cells significantly suppressed faecal microbiota-induced M2b polarization as well as the protumorigenic activity of tumour-associated macrophages in vivo., Conclusion: Faecal microbiota in CRC under malnutrition conditions exhibits specific characteristics that accelerate CRC progression and regulate B cells and macrophages. The use of specific faecal microbial species could be a feasible approach for identifying the malnutrition status of patients and demonstrating the poor prognosis of CRC., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)