Search

By a combination of protein chemistry and recombinant DNA methods a glycine-rich region was found to be located near the carboxyl terminus of the nucleolar specific phosphoprotein, nucleolin, from Novikoff hepatoma (protein C23) and Chinese hamster ovary cells (100-kDa nucleolar protein). A sequence of 192 amino acid residues was derived from partial sequences of cyanogen bromide and N-bromosuccinimide fragments of protein C23 and deduced protein sequence from Chinese hamster ovary cell 100-kDa cDNA sequences. The 66 residues sequenced by protein methods were identical to the corresponding residues deduced by DNA sequencing. The multiple residues of NG,NG-dimethylarginine (DMA) contained in the nucleolin polypeptide were found to be limited to a segment of less than 10 kDa near the carboxyl-terminal end of the protein. This segment also contained internally repeated sequences (e.g. 7 copies of the sequence Gly-Gly-Arg-Gly-Gly were found) which were unrelated to sequences closer to the amino-terminal end. Most arginine residues in this region were surrounded by 2 or 3 glycine residues and were relatively close in sequence to phenylalanine residues.

The significance of osteomedullography was studied in 17 patients with diaphyseal fractures of long bones to determine a rational form of treatment for delayed healing and non-union. Positive osteomedullographic signs were noted in 6 cases of delayed healing and negative signs in 11 cases. All the positive cases united by prolonging the period of immobilization in plaster. Seven of the 11 negative cases were treated by bone grafting. Three patients treated by prolonged immobilization in plaster have not shown evidence of union even after a period of 20 weeks. This simple investigation could be useful in avoiding undue loss of time in the management of delayed union and non-union.

Pelvic urethrotomies were attempted on 32 bulls (12 cattle and 20 buffaloes). Infra-anal urethrotomy was carried out under a tranquilliser, posterior epidural and supra-ischial blocks. Prebulbomuscular urethrotomy was performed under a tranquilliser, posterior epidural and pelvic plexus blocks. Cystoscopy and litholapaxy were attempted to make comparisons. The animals which had been operated on were maintained for one month during which postoperative signs were noted, and after they were killed post mortem, radiographic, macro- and microscopic findings were recorded. From the results it is concluded that infra-anal urethrotomy presents difficulties whereas prebulbomuscular urethrotomy, especially the closed technique, is suitable for cystoscopy, pelvic lithotomy and litholapaxy.

Cytogenetic studies involving six species of spinous Solanums have been made using the parameters of crossability/fertility relationships and chromosome pairing in parental and derived hybrid genomes. These studies have made it possible to integrate hybrid reproductive performance with observed cytological phenomena and draw inferences on concerned intergenomic homeologies. It has been found that species' differences, as they exist, are ascribable to chromosome repatterning, in their divergence and maintenance as distinct taxa. In the light of these studies, the grouping of these species has to be reconsidered.

In 13 dogs that were anesthetized and ventilated through an endotracheal tube, segments of either the intrathoracic (ITT) or extrathoracic trachea (ETT) were distended by over-inflating the sealing cuff within the first half of an inspiration and maintaining this overinflation for two breathing cycles. ITT distension caused a significant prolongation of the expiratory duration (TE) of the first challenged breath in 12 of the 13 dogs. ETT distension prolonged the TE in only 5 of the 13 dogs. An inhibition of inspiration resulting in shortened inspiratory time and reduced peak phrenic amplitude (PPA) was measured in three experiments. In six other experiments, there was only a transient decrease in phrenic discharge, which immediately resumed. In the remaining four experiments, the inspiratory burst was unaffected. After vagotomy, ITT distension shortened TE in 8 of the 13 dogs. ETT distension was ineffective in 10 out of 11 experiments; in the remaining experiment TE was significantly lengthened. No significant effects were elicited on TI and PPA by either ITT or ETT distension. These results could be explained by the known behavior of tracheal receptors and could indicate the presence of extravagal influences.