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The prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea. Prestin belongs to an anion transporter family (SLC26A). It is densely expressed in the OHC lateral plasma membrane and functions as a voltage-dependent motor protein. Analog genes can be found in the genome of nonmammalian species, but their functions in hearing are poorly understood. In the present study, we used the gerbil prestin sequence as a template and identified an analog gene in the bullfrog genome. We expressed the gene in a stable cell line (HEK293T) and performed patch-clamp recording. We found that these cells exhibited prominent nonlinear capacitance (NLC), a widely accepted assay for prestin functioning as a motor protein. Upon close examination, the key parameters of this NLC are comparable to that conferred by the gerbil prestin, and nontransfected cells failed to display NLC. Lastly, we performed patch-clamp recording in HCs of all three hearing organs in bullfrog. HCs in both the sacculus and the amphibian papilla exhibited a capacitance profile that is similar to NLC while HCs in the basilar papilla showed no sign of NLC. Whether or not this NLC-like capacitance change is involved in auditory signal amplification certainly requires further examination; our results represent the first and necessary step in revealing possible roles of prestin in the active hearing processes found in many nonmammalian species. [ABSTRACT FROM AUTHOR]

In geo-distributed cloud storage systems, data replication has been widely used to serve the ever more users around the world for high data reliability and availability. How to optimize the data replica placement has become one of the fundamental problems to reduce the inter-node traffic and the system overhead of accessing associated data items. In the big data era, traditional solutions may face the challenges of long running time and large overheads to handle the increasing scale of data items with time-varying user requests. Therefore, novel offline community discovery and online community adjustment schemes are proposed to solve the replica placement problem in a scalable and adaptive way. The offline scheme can find a replica placement solution based on the average read/write rates for a certain period of time. The scalability can be achieved as 1) the computation complexity is linear to the amount of data items and 2) the data-node communities can evolve in parallel for a distributed replica placement. Furthermore, the online scheme is adaptive to handle the bursty data requests, without the need to completely override the existing replica placement. Driven by real-world data traces, extensive performance evaluations demonstrate the effectiveness of our design to handle large-scale datasets. [ABSTRACT FROM AUTHOR]

Highlights • Both the auditory and limbic brain areas were involved in salicylate-induced tinnitus. • Metabolic activity of the central nervous system was increased in salicylate-induced tinnitus. • GABA A receptor binding was enhanced during the recovery period of the reversible tinnitus. Abstract Tinnitus is relevant to neural hyperactivity in the central nervous system (CNS). Normal quantity and functioning of the γ-aminobutyric acid (GABA) receptor are crucial for maintaining the balance between excitation and inhibition in the brain. In this study, we applied a rat model of tinnitus via long-term salicylate administration. The combination of the gap pre-pulse inhibition of acoustic startle (GPIAS) and pre-pulse inhibition (PPI) tests were used to detect tinnitus-like behavior, and rats receiving 7 or 14 consecutive days of salicylate administration showed evidence of tinnitus. After positron emission tomography (PET) scan, we found that the metabolic activity was increased after salicylate treatment followed by enhanced GABA A receptor binding with cessation of salicylate administration in the auditory cortex (AC), medial prefrontal cortex (mPFC), hippocampus (HP), cingulate cortex (CiC) and insular (InC). The inferior colliculus (IC) showed an elevated metabolic activity with no change in the GABA A receptor binding. All the alterations returned to baseline several days after cessation of salicylate treatment despite a mismatch between the time-course of them. By contrast, we found alterations in neither the metabolic activity nor the GABA A receptor binding in the amygdala (AMY) and cerebellum (CRB). These findings indicate that enhanced neural activity in the auditory and limbic system may contribute to the development of tinnitus, while the hysteretic increase of GABA A receptor binding in specific areas of the CNS may be a compensation for hyperactivity, which may be involved in tinnitus relieving. [ABSTRACT FROM AUTHOR]

The cruise control of high-speed trains is challenging due to the presence of time-varying air resistance coefficients and control constrains. Because the resistance coefficients for high-speed trains are not accurately known and will change with the actual operating environment, the precision of high speed train model is lower. In order to ensure the safe and effective operation of the train, the operating conditions of the train must meet the safety constraints. The most traditional cruise control methods are PID control, model predictive control, and so on, in which the high-speed train model is identified offline. However, the traditional methods typically suffer from performance degradations in the presence of time-varying resistance coefficients. In this paper, an adaptive model predictive control (MPC) method is proposed for cruise control of high-speed trains with time-varying resistance coefficients. The adaptive MPC is designed by combining an adaptive updating law for estimated parameters and a multiply constrained MPC for the estimated system. It is proved theoretically that, with the proposed adaptive MPC, the high-speed trains track the desired speed with ultimately bounded tracking errors, while the estimated parameters are bounded and the relative spring displacement between the two neighboring cars is stable at the equilibrium state. Simulations results validate that proposed method is better than the traditional model predictive control. [ABSTRACT FROM AUTHOR]

The switched shunting resistor circuit is a classical and practical passive equalization scheme that nevertheless suffers from the voltage imbalance after the charging process. It results from the discrepancy of the shunting resistor and the physical characteristics of supercapacitor (SC) cells. In this paper, the distributed voltage equalization control strategies with the proportional-integral (PI) observer for the switched shunting resistor circuit are analyzed and compared. First, the charging and equalizing system is modeled as the switched system and the system observability is proved. The decentralized control and consensus control are analyzed and their shortages are presented. Then, the leader–follower cooperative control is proposed to improve the charging time while keeping the equalization performance. Meanwhile, the PI observer is designed to estimate the internal capacitance voltage of an SC precisely to avoid the effect of equal series resistance. The control convergence is proved by using the Lyapunov stability and switched system theory. The experimental results show that the proposed method has much superiority and wide applicability than the existing methods for equalizing voltages of SCs. [ABSTRACT FROM AUTHOR]

The high-speed train operation process is highly nonlinear and has multiple constraints and objectives, which lead to a requirement for the automatic train operation (ATO) system. In this paper, a hybrid model predictive control (MPC) framework is proposed for the controller design of the ATO system. Firstly, a piecewise linear system with state and input constraints is constructed through piecewise linearization of the high-speed train’s nonlinear dynamics. Secondly, the piecewise linear system is transformed into a mixed logical dynamical (MLD) system by introducing the auxiliary binary variables. For the transformed MLD system, a hybrid MPC controller is designed to realize the precise control under hard constraints. To reduce the online computation complexity, the explicit control law is computed offline by employing the mixed-integer linear programming (MILP) technique. Simulation results validate the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

This study aimed to test the association between the European GWAS-identified risk IQGAP2 SNP rs457717 (A>G) and age-related hearing impairment (ARHI) in a Han male Chinese (HMC) population. A total of 2420 HMC subjects were divided into two groups [group 70+: >70 years ( n = 1306), and group 70−: ≤70 years ( n = 1114)]. The participants were categorised into case and control groups according to Z high scores for group 70− and the severity of hearing loss and different audiogram shapes identified by K-means cluster analysis for group 70+. The IQGAP2 tagSNP rs457717 was genotyped in accordance with the different ARHI phenotypes. The genotype distributions of IQGAP2 (AA/AG/GG) were not significantly different between the case and control groups ( P = 0.613 for group 70−; P = 0.602 for group 70+). Compared with genotype AA, the ORs of genotypes AG and GG for ARHI were not significantly different following adjustment for other environmental risk factors. We demonstrated that the IQGAP2 TagSNP rs457717 (A/G) was not associated with ARHI in HMC individuals. [ABSTRACT FROM AUTHOR]

Lithium-ion batteries have been employed extensively in many important applications in the electronics industry. For safety and reliability, it is extremely critical to get an accurate and early-stage remaining useful life prognostic of lithium-ion batteries. However, battery lifetime predictions are challenging due to the nonlinear battery degradation and the operational diversity among batteries. To increase the prediction accuracy, this paper proposes a hybrid framework combining the model-based method and data-driven method. In this framework, after estimating the battery capacity using online operating data, battery lifetime is predicted by the model-based empirical model as well as the data-driven support vector regression model. For the empirical model, its adaptability is improved by updating the parameters dynamically with particle filters. For the support vector regression model, its performance is optimized by an artificial bee colony algorithm. Finally, a fusion method with cascaded structure is proposed to integrate predictions from these two models, which boosts the prediction accuracy by iteratively exerting two concatenated Kalman filters. The generality and effectiveness of the proposed method are verified on battery data sets provided by NASA and our testing bench, respectively. The experimental results illustrate that the proposed method can improve the prediction accuracy of battery remaining lifetime, especially at the early stage. RMSE and MAE of the proposed hybrid framework are within 4 and 3.5. Compared with two existed hybrid methods, RMSE of prediction can be reduced by at least 7.6%. A reduction of not less than 5.9% in MAE of prediction is achieved. • A hybrid prediction method is proposed to predict RUL at the early stage of batteries. • The proposed method integrates the empirical model and data-driven model through cascaded Kalman filters. • Empirical model is updated by particle filters online to adapt to battery dynamically. • Data-driven model is optimized by artificial bee colony algorithm. [ABSTRACT FROM AUTHOR]

The primary aim of the current study was to examine the social maturity of infants and children with cochlear implants in China. A secondary aim was to document the associated information using a General Condition Questionnaire for further cohort study in this population. This is a retrospective cross-sectional observational study and a pilot study. Between July and December 2017, data from all implantees between the ages of 6 months and 14 years who presented to the Hearing and Speech Center of Shanghai Ninth People's Hospital for mapping were collected. There were 119 non-overlapping cases in total. Data were collected via the Infants-Junior Middle School Students' Social-Life Abilities Scale (S-M scale) and a structured General Condition Questionnaire. The S-M scale was used for social adaptability measurement for children aged between 6 months to 14 yearsold. The General Condition Questionnaire, which was completed by the parents or primary caregivers of the implantees, gathered 3 aspects of personal information: the patient's history of hearing loss, personal history of rehabilitation and their past medical history. Results showed that in 119 cases, 89 implantees (74.8%) were at or above a normal social maturity level. Thirty implantees (25.2%) scored lower than normal on the S-M standard score. Spearman's rank correlation indicated that the age at which hearing loss was noticed, the age of initial rehabilitation and the age at implantation were significantly correlated with the patients' scores on the S-M scale. In summary, most of the cochlear implantees showed normal social maturity, with the exception of 25.2% of implantees who performed at a lower level than their normal hearing peers. These findings suggest potential targets to investigate in future cohort studies in cochlear implantees. [ABSTRACT FROM AUTHOR]

Celery ( Apium graveolens L.) is a popular vegetable crop planted worldwide. In this study, the volatile compound characteristics of three A. graveolens var. dulce varieties (type D), and six A. graveolens var. secalinum varieties (type S), and three breeding selections were analysed and 41 and 73 volatile compounds were identified using GC-MS and GC-IMS, respectively. The results showed that type S emitted more terpenes and phthalides than type D varieties. Both GC-MS and GC-IMS could discriminate between type S and type D. Six potential biomarkers, i.e., sabinene, ( E )-caryophyllene, α-phellandrene, limonene, γ-terpinene, and hexanal, were identified using both models. Both GC-MS and GC-IMS could discriminate between parent and breeding selection. The relative contents of seven (GC-IMS OPLS-DA model) and ten (GC-MS OPLS-DA model) volatile compounds showed over-parent heterosis. This study provides a technical reference for distinguishing celery varieties in the same habitat and preliminarily reveals the aroma relationship between breeding selections and their parents., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Litsea cubeba essential oil (LCEO) has garnered widespread attention due to its robust biological activity. However, challenges such as high volatility, limited water solubility, and low bioavailability impede its application. Nano-emulsion encapsulation technology offers an effective solution to these issues. In this study, we prepared litsea cubeba essential oil nano-emulsion (LCEO-NE) for the first time using whey protein (WP) as the emulsifier through an ultrasonic-assisted method, achieving high efficiency with minimal energy consumption. Transmission electron microscopy and dynamic light scattering analyses revealed that the nanoparticles were uniformly spherical, with a particle size of 183.5 ± 1.19 nm and a zeta potential of -35.5 ± 0.95 mV. Stability studies revealed that LCEO-NE exhibited excellent thermal and salt stability, maintaining its integrity for up to four weeks when stored at 4 °C and 25 °C. In vitro digestion assays confirmed the digestibility of LCEO-NE. Furthermore, evaluation of the DPPH, ABTS, and antimicrobial activities revealed that LCEO-NE displayed superior bacteriostatic and antioxidant properties compared to LCEO. Scanning electron microscopy elucidated that its bacteriostatic effect involved the disruption of bacterial microstructure. Hemocompatibility and cytotoxicity assays demonstrated the safety of LCEO-NE within the effective concentration range. This research supports the utilization of nanoparticles for encapsulating LCEO, thereby enhancing its stability and bioactivity, and consequently expanding its applications in the food and pharmaceutical industries., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier B.V.)

Celery seeds contain various bioactive compounds and are commonly used as a spice and nutritional supplement in people's daily lives. The quality of celery seeds sold on the market varies, and their regions of production are unclear. This study evaluated the metabolites of Chinese celery seeds from three production regions using HS-SPME-GC-MS, HS-GC-IMS, and UPLC-ESI-MS/MS. The results indicate that GC-IMS analysis obtained a metabolic profile different from that detected using GC-MS. Terpenoids, polyphenols, coumarins, and phthalides are the main bioactive compounds in celery seeds. The production region significantly affects the metabolic characteristics of celery seeds. Based on GC-MS data, GC-IMS data, and LC-MS data, the variation analysis screened 6, 12, and 8 metabolites as potential characteristic metabolites in celery seeds related to the production region, respectively. According to the aromatic characteristics of the characteristic metabolites, seeds from the HCQ region and HZC region have a strong herbal, woody, celery, and turpentine aroma. The concentration of secondary metabolites was highest in the seeds from the HCQ region followed by the HZC region, and it was the lowest in the JJC region. Altogether, this study investigates how geographical origins influence the metabolomic profile of celery seeds. The results can be used to guide the planting and harvesting of celery seeds in suitable regions.

Traditional hydrogels are usually weak and brittle, which limit their application in articular cartilage replacement because cartilage is generally strong, tough, and elastic in nature. Therefore, it is highly desirable to construct hydrogels to mimic the mechanical properties of the native articular cartilage. Herein, in this work, poly(vinyl alcohol)/polyacrylamide (PVA/PAM) DN hydrogels were prepared by in situ polymerization, which were then treated with Hofmeister series ions (Cit 3- , SO 4 2- , and Cl - ) to achieve H-PVA/PAM DN hydrogels. Among the three Hofmeister ions, the DN hydrogel treated with Cit 3- (named PVA/PAM-Cit) showed the densest microstructure and the highest crystallinity degree. In this context, PVA/PAM-Cit exhibited a tensile strength of 18.9 ± 1.6 MPa, a compressive strength of 102.3 ± 7.9 MPa, a tensile modulus of 10.6 ± 2.1 MPa, a compressive modulus of 8.9 ± 0.8 MPa, and a roughness of 66.2 ± 4.2 MJ m -3 , respectively, which were the highest strength and modulus, and the second highest toughness when compared with those of the reported PVA and PVA based DN hydrogels so far. It also showed an extreme high elasticity, which could maintain a stress of 99.2% after 500 cycles of fatigue testing. Additionally, PVA/PAM-Cit can promote the adhesion, spreading and proliferation of chondrocytes. These results verify that such a strong, tough, and elastic hydrogel could be a novel candidate material for articular cartilage replacement.

Symmetric Positive Definite (SPD) matrices have received wide attention in machine learning due to their intrinsic capacity to encode underlying structural correlation in data. Many successful Riemannian metrics have been proposed to reflect the non-Euclidean geometry of SPD manifolds. However, most existing metric tensors are fixed, which might lead to sub-optimal performance for SPD matrix learning, especially for deep SPD neural networks. To remedy this limitation, we leverage the commonly encountered pullback techniques and propose Adaptive Log-Euclidean Metrics (ALEMs), which extend the widely used Log-Euclidean Metric (LEM). Compared with the previous Riemannian metrics, our metrics contain learnable parameters, which can better adapt to the complex dynamics of Riemannian neural networks with minor extra computations. We also present a complete theoretical analysis to support our ALEMs, including algebraic and Riemannian properties. The experimental and theoretical results demonstrate the merit of the proposed metrics in improving the performance of SPD neural networks. The efficacy of our metrics is further showcased on a set of recently developed Riemannian building blocks, including Riemannian batch normalization, Riemannian Residual blocks, and Riemannian classifiers.

Celery juice is rich in bioactive constituents, has good health properties, and is becoming much more popular, with its demand continuing to rise. The results of this study show that celery juice from Chinese cultivars contains more bioactive constituents, whereas celery cultivars from the United States and European countries have a higher juice yield. Compared with the other juices, the juices of five cultivars may taste sweeter, and the juices of three cultivars had a higher antioxidant capacity. The juices of six cultivars (three with the highest antioxidant capacity and three with the lowest antioxidant capacity) were selected to analyze bioactive constituents by LC/MS and GC/MS. A total of 71 phenolic acids, 38 flavonoids, 18 coumarins, 41 terpenoids, and 11 phthalides were detected in the juices of the six celery cultivars. The contents of 14 compounds had a more than 10-fold difference among these celery juices. This study first evaluated the comprehensive quality of the juices made from 26 celery cultivars and then analyzed the differences in bioactive constituents in the juices of6 celery cultivars. These findings provide information for the further study on the health functions of celery juice and can also guide celery juice production and celery breeding.

Purpose: This study aimed to quantitatively assess the incidence of hearing loss in relation to age in individuals with biallelic p.V37I variant in GJB2., Methods: Population screening of the biallelic p.V37I variant was performed in 30,122 individuals aged between 0 and 97 years in Shanghai. Hearing thresholds of the biallelic p.V37I individuals and the controls were determined by click auditory brainstem response or pure tone audiometry., Results: Biallelic p.V37I was detected in 0.528% (159/30,122) of the subjects. Of the biallelic p.V37I newborns, 43.91% (18/41) passed their distortion-product otoacoustic emissions-based newborn hearing screening or had hearing thresholds lower than 20 decible above normal hearing level. The older newborns had elevated hearing thresholds, with increasing incidence of 9.52%, 23.08%, 59.38%, and 80.00% for moderate or higher grade of hearing loss in age groups of 7 to 15 years, 20 to 40 years, 40 to 60 years, and 60 to 85 years, respectively. Their hearing deteriorated at a rate of 0.40 dB hearing level per year on average; males were more susceptible, and deterioration occurred preferentially at higher sound frequencies., Conclusion: The biallelic p.V37I variant is associated with steadily progressive hearing loss with increasing incidence over the course of life. Most of the biallelic p.V37I individuals may develop significant hearing loss in adulthood and, can benefit from early diagnosis and intervention through wide-spread genetic screening., Competing Interests: Conflict of Interest The authors declare no conflicts of interest. The authors declare that they do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Auditory brainstem response (ABR) serves as an objective indication of auditory perception at a given sound level and is nowadays widely used in hearing function assessment. Despite efforts for automation over decades, ABR threshold determination by machine algorithms remains unreliable and thereby one still relies on visual identification by trained personnel. Here, we described a procedure for automatic threshold determination that can be used in both animal and human ABR tests. The method terminates level averaging of ABR recordings upon detection of time-locked waveform through cross-correlation analysis. The threshold level was then indicated by a dramatic increase in the sweep numbers required to produce "qualified" level averaging. A good match was obtained between the algorithm outcome and the human readouts. Moreover, the method varies the level averaging based on the cross-correlation, thereby adapting to the signal-to-noise ratio of sweep recordings. These features empower a robust and fully automated ABR test., Competing Interests: The authors declare no competing interests., (© 2021 The Author(s).)

Objective: To evaluate the efficacy of modified mini-mental state examination（MMSE） in elderly patients with severe to profound hearing loss. Methods: A total of 24 elderly patients with severe to profound hearing loss from April to June 2019 were involved. Severe to profound hearing loss was defined by a pure-tone average of hearing thresholds at 0.5, 1, 2, and 4 kHz in the better-hearing ear. Modified and original MMSE was completed for the patients at first visit. Two weeks later, they all return hospital and MMSE were carried out by the other method. The aggregate score and subitem score of MMSE by modified and original instrument were analyzed. The degree of cognitive impairment evaluated by two methods were compared. Results: Among the 24 patients, the mean MMSE score by routine method and improved method were 10.88±9.70 and 25.29±3.70（ P <0.01）. The average score of sub-items of MMSE with routine method and improved method are as follows: orientation 3.21±4.03 vs 8.71±1.92（ P <0.01）, registration 1.04±1.33 vs 2.79±0.51（ P <0.01）, attention and calculation 1.63±2.02 vs 4.00±1.41（ P <0.01）, recall 0.79±1.14 vs 2.50±0.72（ P <0.01）, language 4.21±2.11 vs 7.29±1.33（ P <0.01）. Compared with the routine method, the degree of cognitive impairment getting better in 83.3% patients with improved method, meanwhile, 16.7% of the patients remain the same and no deterioration. Conclusion: The routine method of MMSE should be improved to seek the real cognitive state of the patients with severe to profound hearing loss., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Active braking control systems in high-speed trains are vital to ensure safety and are intended to reduce brake distances and prevent the wheels from locking. The slip ratio, which represents the relative difference between the wheel speed and vehicle velocity, is crucial to the design and successful implementation of active braking control systems. Slip ratio estimation and active braking control are challenging owing to the uncertainties of wheel-rail adhesion and system nonlinearities. Therefore, this paper proposes a novel adaptive slip ratio estimation approach for the active braking control based on an improved extended state observer. The extended state observer is developed through the augmentation of the system state-space to estimate the unmeasured train states as well as the model uncertainty. The accurate slip ratio is estimated using the observed extended states. Furthermore, the adaptability of the observer is improved by introducing the beetle antennae search algorithm to determine the optimal observer parameters. Finally, a feedback linearization braking control law is established to stabilize the closed-loop system due to its potential in coping with nonlinearities, which benefits the proven theoretical bounded stability. Experimental results validate the effectiveness of the proposed method., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 ISA. Published by Elsevier Ltd. All rights reserved.)

Objective: To compare the hearing screening results of two-step transient evoked otoacoustic emissions (TEOAE) and one-step automatic auditory brainstem response (AABR) in non-risk newborns, and to explore a more suitable hearing screening protocol for infants discharged within 48 h after birth in remote areas of China. Methods: To analyze the age effect on pass rate for hearing screening, 2005 newborns were divided into three groups according to screening time after birth: <24, 24-48, and 48-72 h. All subjects received TEOAE + AABR test as first hearing screen, and those who failed in any test were rescreened with TEOAE + AABR at 6 weeks after birth. The first screening results of AABR and TEOAE were compared among the three groups. The results of two-step TEOAE screening and one-step AABR screening were compared for newborns who were discharged within 48 h. The time spent on screening was recorded for TEOAE and AABR. Results: The pass rate of TEOAE and AABR increased significantly with the increase of first screening time ( P < 0.05), and the false positive rate decreased significantly with the increase of first screening time ( P < 0.05). The failure rate of first screening of AABR within 48 h was 7.31%, which was significantly lower than that of TEOAE (9.93%) ( P < 0.05). The average time spent on AABR was 12.51 ± 6.36 min, which was significantly higher than that of TEOAE (4.05 ± 1.56 min, P < 0.05). The failure rate of TEOAE two-step screening was 1.59%, which was significantly lower than one-step AABR. Conclusions: Compared with TEOAE, AABR screening within 48 h after birth can reduce the failure rate and false positive rate of first screening. However, compared with TEOAE two-step screening, one-step AABR screening has higher referral rate for audiological diagnosis. In remote areas of China, especially in hospitals with high delivery rate, one-step AABR screening is not feasible, and two-step TEOAE screening protocol is still applicable to UNHS screening as more and more infants discharged within 48 h after birth., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Sheng, Zhou, Wang, Yu, Liu, Liang, Zhou, Wu, Tang and Huang.)

Among the vertebrate lineages with different hearing frequency ranges, humans lie between the low-frequency hearing (1 kHz) of fish and amphibians and the high-frequency hearing (100 kHz) of bats and dolphins. Little is known about the mechanism underlying such a striking difference in the limits of hearing frequency. Prestin, responsible for cochlear amplification and frequency selectivity in mammals, seems to be the only candidate to date. Mammalian prestin is densely expressed in the lateral plasma membrane of the outer hair cells (OHCs) and functions as a voltage-dependent motor protein. To explore the molecular basis for the contribution of prestin in hearing frequency detection, we collected audiogram data from humans, dogs, gerbils, bats, and dolphins because their average hearing frequency rises in steps. We generated stable cell lines transfected with human, dog, gerbil, bat, and dolphin prestins (hPres, dPres, gPres, bPres, and nPres, respectively). The non-linear capacitance (NLC) of different prestins was measured using a whole-cell patch clamp. We found that the Q max / C lin of bPres and nPres was significantly higher than that of humans. The V 1 / 2 of hPres was more hyperpolarized than that of nPres. The z values of hPres and bPres were higher than that of nPres. We further analyzed the relationship between the high-frequency hearing limit ( F max ) and the functional parameters ( V 1 / 2 , z , and Q max / C lin ) of NLC among five prestins. Interestingly, no significant correlation was found between the functional parameters and F max . Additionally, a comparative study showed that the amino acid sequences and tertiary structures of five prestins were quite similar. There might be a common fundamental mechanism driving the function of prestins. These findings call for a reconsideration of the leading role of prestin in hearing frequency perception. Other intriguing kinetics underlying the hearing frequency response of auditory organs might exist., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Wang, Ma, Lu, Cui, Chen, Wu and Huang.)

Objectives: The objective of the study was to identify the acute high-intensity recreational noise-induced effects on auditory function, especially the cochlear synaptopathy-related audiological metrics, in humans with normal hearing., Methods: This prospective cohort study enrolled 32 young adults (14 males and 18 females); the mean age was 24.1 ± 2.4 years (ranging from 20 to 29). All participants with normal hearing (audiometric thresholds ≤25 dB HL at frequencies of 0.25, 0.5, 1, 2, 3, 4, 6, and 8 kHz for both ears) had already decided to participate in the outdoor music festival. Participants were asked to measure the noise exposure dose and complete auditory examinations, including the air-conduction pure-tone audiometry (PTA), distortion product otoacoustic emission (DPOAE), contralateral suppression (CS) on transient evoked otoacoustic emission (TEOAE), auditory brainstem response (ABR) test and Mandarin Hearing in Noise Test (MHINT), at baseline and 1 day and 14 days after music festival noise exposure., Results: The mean time of attending the music festival was 7.34 ± 0.63 h (ranging from 6.4 to 9.5), the mean time-weighted average (TWA) of noise exposure dose was 93.2 ± 2.39 dB(A) (ranging from 87.9 to 97.7). At neither 1 day nor 14 days post exposure, there were no statistically significant effects on PTA thresholds, DPOAE amplitudes, CS on TEOAEs, or MHINT signal-to-noise ratios (SNRs) of acute outdoor music festival noise exposure, regardless of sex. While the ABR wave I amplitudes significantly decreased at 1 day after exposure and recovered at 14 days after exposure, the exposed/unexposed ABR wave I amplitude ratio was significantly correlated with MHINT SNR change at 1 day after exposure, although it was not correlated with the noise exposure dose., Conclusion: In young adults with normal hearing, we found the self-compared decrement of ABR wave I amplitudes at 1 day post acute recreational noise exposure at high intensity, which also contributes to the change in speech perceptual ability in noisy backgrounds. This study indicated that auditory electrophysiological metric changes might be a more sensitive and efficient indicator of noise-induced cochlear synaptic dysfunction in humans. More attention should be paid to the recreational noise-induced cochlear synaptopathy and auditory perceptual disorder., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Wang, Yang, Qian, Hong, Wang, Huang and Wu.)

Background: The definition of notched audiogram for noise-induced hearing loss (NIHL) is presently based on clinical experience, but audiometric phenotypes of NIHL are highly heterogeneous. The data-driven clustering of subtypes could provide refined characteristics of NIHL, and help identify individuals with typical NIHL at diagnosis. Methods: This cross-sectional study initially recruited 12,218 occupational noise-exposed employees aged 18-60 years from two factories of a shipyard in Eastern China. Of these, 10,307 subjects with no history of otological injurie or disease, family history of hearing loss, or history of ototoxic drug use were eventually enrolled. All these subjects completed health behavior questionnaires, cumulative noise exposure (CNE) measurement, and pure-tone audiometry. We did data-driven cluster analysis (k-means clustering) in subjects with hearing loss audiograms ( n = 6,599) consist of two independent datasets ( n = 4,461 and n = 2,138). Multinomial logistic regression was performed to analyze the relevant characteristics of subjects with different audiometric phenotypes compared to those subjects with normal hearing audiograms ( n = 3,708). Results: A total of 10,307 subjects (9,165 males [88.9%], mean age 34.5 [8.8] years, mean CNE 91.2 [22.7] dB[A]) were included, 3,708 (36.0%) of them had completely normal hearing, the other 6,599 (64.0%) with hearing loss audiograms were clustered into four audiometric phenotypes, which were replicable in two distinct datasets. We named the four clusters as the 4-6 kHz sharp-notched, 4-6 kHz flat-notched, 3-8 kHz notched, and 1-8 kHz notched audiogram. Among them, except for the 4-6 kHz flat-notched audiogram which was not significantly related to NIHL, the other three phenotypes with different relevant characteristics were strongly associated with noise exposure. In particular, the 4-6 kHz sharp-notched audiogram might be a typical subtype of NIHL. Conclusions: By data-driven cluster analysis of the large-scale noise-exposed population, we identified three audiometric phenotypes associated with distinct NIHL subtypes. Data-driven sub-stratification of audiograms might eventually contribute to the precise diagnosis and treatment of NIHL., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Wang, Qian, Yang, Shi, Hong, Han, Li, Lin, Huang and Wu.)

This study was designed to investigate the effects of the aging process on peripheral and central auditory functions in adults with normal hearing. In this study, 149 participants with normal hearing were divided into four groups: aged 20-29, 30-39, 40-49 and 50-59 years for statistical purposes. Electrocochleography (EcochG), transient evoked otoacoustic emissions (TEOAE), Mandarin Hearing in Noise Test (MHINT) and the Gap Detection Test (GDT) were used. Our study found: (1) MHINT is significantly associated with aging (left ear R 2 =0.29, right ear R 2 =0.35). (2) TEOAE amplitude, TEOAE contralateral acoustic stimulation (CS) amplitude, EcochG action potential (AP), EcochG AP latency, EcochG summating potential (SP) and GDT progressively declined with age. (3) The EcochG SP/AP has no statistically significant difference among different age groups. (4) The peripheral auditory function of the right ear declines more slowly than that of the left ear. (5) Hypofunction of the central auditory system accelerates after age 40. The results demonstrate: (1) The age-related decline in the ability of speech recognition in a noisy environment may be the most sensitive indicator that reflects auditory function. (2) The decline of central auditory function is independent of peripheral auditory function, according to the auditory characteristics of the right ear. (3) Auditory function needs to be assessed individually to allow early prevention before age 40., Competing Interests: None., (AJTR Copyright © 2021.)

Objective: To evaluate the auditory and speech benefit of bimodal stimulation for prelingual deafened cochlear implantation recipients., Study Design: Retrospective and comparative study., Setting: Tertiary referral center., Patients: Fifty-six children with bilateral prelingual profound sensorineural hearing loss were enrolled, including 28 consecutive children with unilateral cochlear implantation (CI group), and 28 consecutive children with bimodal stimulation (BI group) who used an additional hearing aid (HA) in the contralateral ear., Main Outcome Measures: Hearing assessments included the Infant-Toddler Meaningful Auditory Integration Scale (IT-MAIS) and Categories of Auditory Performance (CAP). Speech evaluations included the Meaningful Use of Speech Scale (MUSS), and Speech Intelligibility Rating (SIR). These measurements were evaluated at the first mapping of cochlear implants and 0.5, 1, 3, 6, 12, 18, 24 months after. Data were analyzed by repeated measures analysis., Results: The mean ages of BI and CI groups were similar (17.6 ± 6.87 vs 19.0 ± 8.10 months, p = 0.497). The initial scores for hearing and speech assessments showed no differences between the two groups, apart from IT-MAIS (2.46 ± 0.631 in BI group vs 0.50 ± 0.279 in CI group, p = 0.004). The auditory and speech development over time were different in the two groups as seen in IT-MAIS (p < 0.001), CAP (p = 0.029), MUSS (p < 0.001), and SIR (p < 0.001). A continuing but stable difference was observed in CAP, MUSS, and SIR at 3, 18, and 12 months after the first mapping, respectively. In addition, the BI group had better IT-MAIS scores at 3 and 6 months compared with the CI group; however, the difference was not significant after 12 months., Conclusion: Bimodal stimulation is beneficial for prelingually deafened CI recipients who have minimal contralateral residual hearing when bilateral CIs are not available. Hearing aid use in the contralateral ear might be recommended for children after unilateral cochlear implantation to facilitate the development of auditory and speech skills.

Objective: To evaluate the cognitive function of patients with presbycusis, and to explore the relationship among age, hearing loss, speech recognition ability and cognitive function. Method: A total of 131 elder participated in this study. Based on hearing threshold, all participants were divided into the elderly hearing loss group（66 cases） and the control group（65 cases with normal hearing）. ① The pure tone audiometry test was completed to obtain the average hearing threshold of 0.5, 1, 2, and 4 kHz; ② The single-syllable vocabulary in Mandarin Speech Test Materials was used to test the maximum speech recognition rate and the speech recognition rate at 60 dB HL in a quiet environment about the better ear; ③ Chinese version of the Mini-mental State Examination（MMSE） was used to assess the subject's cognitive function; ④ Use spearman correlation coefficient to analyze the correlation between age, average hearing threshold, maximum speech recognition rate and the MMSE score; ⑤ Use GLM to analyze the effects of gender, deafness time, and education level on the cognitive function in presbycusis patients. Result: ① The proportion of mild cognitive dysfunction（<27 points）（16.7%） in the elderly hearing loss group was higher than the control group（10.8%）, but there was no significant difference（ P >0.05）; ②The MMSE score decreased with age（ P <0.05）, and there was a significant correlation between MMSE score and age（the control group: r =-0.424, P =0.000; the elderly hearing loss group: r =-0.277, P =0.024）; ③There was a significant difference in MMSE scores between groups with different average hearing threshold levels（ P <0.05）, and there was a significant correlation between the MMSE score and the average hearing threshold in the elderly hearing loss group（ r =-0.375, P <0.01）; ④The MMSE score of the elderly hearing loss group with a low speech recognition rate at 60 dB HL（<50%） was significantly lower than the group with a high speech recognition rate（≥50%）, and there was a significant correlation between MMSE score and maximum speech recognition rate（ r =0.402, P =0.001）; ⑤The average age and hearing threshold of the MCI in the elderly hearing loss group were significantly higher than the normal cognitive function group（27-30 points）（ P <0.05）, and the average maximum speech recognition rate was significantly lower than the normal cognitive function group（ P <0.05）; ⑥Analyzed by the GLM, the deafness time and education level have significant effects on MMSE scores（β=-0.066, t =-2.98, P <0.01; β =0.286 9, t =2.39, P <0.05）, but gender have no significant effect on MMSE scores（ β =0.277, t =1.46, P =0.143）. Conclusion: The age, hearing loss and speech recognition ability of presbycusis patients was significantly correlated with cognitive function. The incidence rate of MCI in presbycusis patients is higher than that in elderly normal hearing subjects. Deafness time is a risk factor of mild cognitive impairment, while education is a protective factor., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Objective: The aim of this study was to investigate the satisfaction of age-related hearing loss (ARHL) or presbycusis patients with individual, accurate, and precise fitting progress, which is a priority for bilateral hearing aids, and to explore the related influencing factors and their role in predicting the efficiency of hearing aids., Methods: A total of 73 cases of presbycusis patients aged 60-95 years old underwent pure tone audiometry and speech recognition ability examination to obtain the pure tone audiometry of the better ear (BPTA) and maximum speech recognition rate of the better ear (BSRR) in quiet environment before hearing aid fitting. Audiologists evaluated the efficiency and satisfaction of participants according to the International Outcome Inventory for Hearing Aids (IOI-HA) questionnaire scores by face-to-face or telephone investigations after using the hearing aids for at least 3 months. The data were analyzed related to possible influencing factors., Results: Total satisfaction percentage according to IOI-HA scores was 86.3%. There was no significant correlation between age, first fitting age, unilateral or bilateral hearing aids, BPTA, and IOI-HA total score. BSRR was strongly correlated to total IOI-HA scores ( r =0.768). According to the multiple linear regression analysis, BPTA and BSRR both had a statistically significant effect on the total IOI-HA scores after hearing aid intervention., Conclusion: ARHL patients with accurate hearing aid fitting will have high satisfaction and bilateral hearing aids are better than unilateral ones. Age and first fitting age are not meaningful to satisfaction with hearing aids. A higher maximum speech recognition rate before hearing aids fitting could predict better efficiency and satisfaction with hearing aids. Therefore, completing speech recognition ability examination before fitting would make a great contribution to the efficiency of hearing aids, and help ARHL patients have realistic expectations., Competing Interests: Disclosure The authors report no conflicts of interest in this work.

Tinnitus is associated with neural hyperactivity, which is regulated by neuronal plasticity in the auditory central system, especially the auditory cortex (AC). Excitatory neurons constitute approximately 70-85% of the total populations of neuronal cells. However, few reports have focused on the AMPA receptor (AMPAR) and the GABA A receptor (GABA A R) on the excitatory neuron in animal model of tinnitus. In this study, we gave rats a single or long-term of salicylate administrations. The tinnitus-like behavior was assessed by combination of the gap prepulse inhibition of acoustic startle (GPIAS) and the pre-pulse inhibition (PPI) tests. Using immunofluorescent staining, we examined whether the AMPAR and the GABA A R on the calcium/calmodulin-dependent protein kinase IIα (CaMKIIα) -labeled excitatory neurons in the auditory cortex underwent changes following salicylate treatment. The rats with 14 days of salicylate administration showed evidence of experiencing tinnitus, while the rats receiving a single dose of salicylate manifested no tinnitus-like behavior. Furthermore, the AMPAR and GABA A R responded in a homeostatic manner after a single dose of salicylate while those showing in a Hebbian way after long-term salicylate administration. Thus, the different patterns of plasticity change in cortical excitatory neurons might affect the generating of salicylate-induced tinnitus., Competing Interests: None.

Riemannian manifolds have been widely employed for video representations in visual classification tasks including video-based face recognition. The success mainly derives from learning a discriminant Riemannian metric which encodes the non-linear geometry of the underlying Riemannian manifolds. In this paper, we propose a novel metric learning framework to learn a distance metric across a Euclidean space and a Riemannian manifold to fuse average appearance and pattern variation of faces within one video. The proposed metric learning framework can handle three typical tasks of video-based face recognition: Video-to-Still, Still-to-Video and Video-to-Video settings. To accomplish this new framework, by exploiting typical Riemannian geometries for kernel embedding, we map the source Euclidean space and Riemannian manifold into a common Euclidean subspace, each through a corresponding high-dimensional Reproducing Kernel Hilbert Space (RKHS). With this mapping, the problem of learning a cross-view metric between the two source heterogeneous spaces can be converted to learning a single-view Euclidean distance metric in the target common Euclidean space. By learning information on heterogeneous data with the shared label, the discriminant metric in the common space improves face recognition from videos. Extensive experiments on four challenging video face databases demonstrate that the proposed framework has a clear advantage over the state-of-the-art methods in the three classical video-based face recognition scenarios.

Hypothesis: We investigated whether salicylate induces tinnitus through alteration of the expression levels of brain-derived neurotrophic factor (BDNF), proBDNF, tyrosine kinase receptor B (TrkB), cAMP-responsive element-binding protein (CREB), and phosphorylated CREB (p-CREB) in the auditory cortex (AC)., Background: Salicylate medication is frequently used for long-term treatment in clinical settings, but it may cause reversible tinnitus. Salicylate-induced tinnitus is associated with changes related to central auditory neuroplasticity. Our previous studies revealed enhanced neural activity and ultrastructural synaptic changes in the central auditory system after long-term salicylate administration. However, the underlying mechanisms remained unclear., Methods: Salicylate-induced tinnitus-like behavior in rats was confirmed using gap prepulse inhibition of acoustic startle and prepulse inhibition testing, followed by comparison of the expression levels of BDNF, proBDNF, TrkB, CREB, and p-CREB. Synaptic ultrastructure was observed under a transmission electron microscope., Results: BDNF and p-CREB were upregulated along with ultrastructural changes at the synapses in the AC of rats treated chronically with salicylate (p < 0.05, compared with control group). These changes returned to normal after 14 days of recovery (p > 0.05)., Conclusion: Long-term administration of salicylate increased BDNF expression and CREB activation, upregulated synaptic efficacy, and changed synaptic ultrastructure in the AC. There may be a relationship between these factors and the mechanism of tinnitus.

Tinnitus is the most common disease in Otology, and extremely difficult for treatment in clinic, abnormal events in the cochlea (the abnormal events can result in abnormal neuronal activity in central auditory pathways that can then be finally perceived as tinnitus). Neuroplasticity events at the auditory cortex (AC) have been reported to include hyperactive of cortical neurons and an increase in neuronal synchronization. Our recent studies showed the changes markedly, in the expression of the excitatory glutamate receptor subtype NR2B in mRNA and protein levels, and also some changes in synaptic ultrastructure of neurons in auditory cortex of tinnitus animal. We propose that the mechanisms of tinnitus centralization may arise from abnormal events in the cochlea, and result in abnormal neuronal activity at multiple levels which promote abnormal propagation of neural activity in the central auditory pathway. The plastic change may be positive and adaptive as with learning or memory, or in the compensation after abnormal events in the cochlea that results in new neuronal networks that restore normal function. Alternatively, the neuroplasticity changes might be maladaptive leading perhaps to an imbalance in excitatory and inhibitory events in the brain. Indeed, tinnitus may be the consequence of such maladaptive neuroplasticity brain alterations (synaptic structure) has even gone a step further and described tinnitus as the perceptual manifestation of plastic brain changes that result in abnormal neuronal activity. The neuroplasticity changes may also make tinnitus persists, eventually leading to the existence of tinnitus cochlear-originated in the central pathway. They may also extend to non-sensory areas of the brain giving rise to the attentional and emotional aspects that often accompany the disorder. New pathophysiological insights maybe prompt the development of management approaches to directly target the neuroplasticity processes correlates of tinnitus.

Recessive mutations in SLC26A4 and in rarer cases double heterozygous mutations of FOXI1/SLC26A4 and KCNJ10/SLC26A4 lead to hearing impairment associated with enlarged vestibular aqueduct (EVA), the most common inner ear malformation. In our large cohort study, we addressed several important questions to the molecular etiology of this disorder. The overall prevalence of SLC26A4 mutations in nonsyndromic childhood sensorineural hearing loss (11.2%, 37/330) were determined by sequencing of SLC26A4 in 330 hearing impaired children who did not undergo inner ear radiologic imaging prior to their genetic test. The penetrance of EVA in bi-allelic SLC26A4 mutation carriers (100%, 37/37) was determined by follow-up computed tomography scanning. Combined with the study of 140 additional probands diagnosed with nonsyndromic EVA, we characterized the mutation spectrum of SLC26A4 in East China, which consisted of 19 novel SLC26A4 mutations and differed from those reported in other regions of China., (Copyright © 2013 Wiley Periodicals, Inc.)

Objective: The goal of this study was to investigate the prevalence of delayed-onset hearing loss in preschool children who previously passed newborn hearing screening in Shanghai, China., Methods: Between October 2009 and September 2010, 21,427 preschool children were enrolled from five areas of Shanghai, who had passed newborn hearing screening. Children were screened for delayed-onset hearing loss with pediatric audiometers. Children with positive results in initial and re-screening tests were assessed audiologically and for risk indicators according to the Year 2007 Statement of the American Joint Committee on Infant Hearing (JCIH)., Results: During the study period, 445 children (2.08%) were referred for audiologic assessment; 16 (0.75/1000, 95% CI 0.38-1.12) had permanent delayed-onset hearing loss. Of these, five (0.23/1000) had bilateral moderate hearing loss; seven (0.33/1000) had mild bilateral hearing loss; and four (0.19/1000) had unilateral moderate or mild hearing loss. JCIH risk indicators were established for six children (6/16, 37.5%), including parental concern regarding speech and language developmental delay, neonatal intensive care unit with assisted ventilation or hyperbilirubinemia, recurrent otitis media with effusion, craniofacial malformation, and family history (n=1 for each). The remaining ten (62.5%) had no related risk factors., Conclusions: A significant proportion of preschool children have undiagnosed delayed-onset hearing loss. Hearing screening in preschool is recommended for an early detection., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)

Objective: To find the characteristic of tympanogram results obtained from normative neonates 2 - 7 days about low frequency (226 Hz) probe tone, and to determine the normative values for tympanometric variables., Method: Transient evoked otoacoustic emission (TEOAE) screening were performed by using AccuScreen Pro instrument for 135 neonates. Among them, 105 neonates passed the TEOAE screening in both ears. The 226 Hz probe tone tympanograms and their normative values were obtained from them using GSI-33 middle ear analyzer., Result: The 226 Hz tympanometric data for the 105 neonates (210 ears) passed the TEOAE screening in both ears showed a double-peaked tympanogram in 202 ears (96.19%), a single-peaked tympanogram in 8 ears (3.81%). For double-peaked tympanogram in 202 ears, the tympanometric normative values was below: the first peak admittance is (0.91 +/- 0.18)mmho, Tpp is about (18.02 +/- 12.26)daPa; the second peak admittance is (1.05 +/- 0.23)mmho, Tpp is about (-35.05 +/- 16.80) daPa; the admittance of notch between the two peaks is (0.74 +/- 2.57)mmho, its pressure is about (0.37 +/- 7.61) daPa. Vec is about (0.50 +/- 0.08)ml., Conclusion: The 226 Hz tympanograms obtained from this cohort may serve as a guide for evaluating middle ear function in neonates.

Objective: To investigate the hearing at extended high frequencies of patients with obstructive sleep apnea-hypopnea syndrome (OSAHS)., Method: The youth group, adult group and OSAHS group were tested with pure tone audiometry and high frequency audiometry, and the response ratio was calculated. The results were statistically analysed with chi2-test and ANOVE., Result: In youth group, thresholds of pure tone audiometry in 4, 6, 8 kHz were increased slightly (P < 0.05). The hearing lose gradually with aging in adult group (P < 0.05). The high frequencies thresholds increased with the frequency increasing in youth group (P < 0.05). For each frequencies, the thresholds increased with aging (P < 0.05). At common frequencies hearing loss appeared in OSAHS group (P < 0.05), and more with aging (P < 0.01). The high frequency thresholds increased obviously when OSAHS group comparing with the adult group (P < 0.01), and the indicating ratio decreased obviously (P < 0.05)., Conclusion: The common and high frequency thresholds in OSAHS group of the same age were worse than that of the adult group and it could provide the reference for the clinic.

In order to observe the DPOAE of guinea pigs under awake and anesthetic conditions, we invesitigated the amplitude of distortion product emission and I/O function curves and made a comparative analysis of their changes under different conditions. It was found that the amplitude of DPOAE and I/O curves did not show difference in the guinea pigs under awake and anesthetics conditions. We deem it credible and simple to measure DPOAE in the guinea pigs under awake condition, which is similar to measuring DPOAE in the guinea pigs under physiological condition.

Objective: To investigate the psychological factor of tinnitus and assess the possible influenced aspects., Method: To inspect the 95 cases with tinnitus by SCL-90, the 9 items were divided to 10 factors and compared to the Chinese normalizations., Result: There were significant difference between 4 factors including: somatization, depression, anxiety, compel symptom and normalization (P<0.05). There were correlation between the emotion, age and educational level and psychological dysfunction of tinnitus., Conclusion: Psychoprophylaxis is the important factors to tinnitus, and the major influenced aspects are age, educational level and emotional stability.