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beta-D-N-Acetylglucosaminidase isozymes were separated and assayed in the plasma of control healthy individuals and pregnant women by an automated method consisting in chromatofocusing on polybuffer exchanger PBE-94 column, flow-through fluorimetric determination of activity and computer assisted quantification. Under the established optimal conditions the method fractionated beta-D-N-acetylglucosaminidase into four isozymes. A, I2, I1 and B, with the analytical coefficients of variation of 1.8, 2.2, 6.4 and 4.1%, respectively. Duration of a single analysis was 25 min including washing, and 10-15 successive runs could be performed on the same column with good reproducibility. A linear activity response was observed from 1-5 microliters of plasma (depending on the individual isozyme) to 50 microliters, and the detection limit was 0.016 mUnits. Isozyme A was heat labile. Upon sialidase treatment, isozymes A, I2 and I1 released sialic acid and were eluted from the column at less acidic pHs. In healthy individuals isozymes A, I2, I1 and B covered about 62.8, 6.9, 15.0 and 15.1% of the total beta-D-N-acetyl-glucosaminidase activity, respectively. During pregnancy the plasma concentration of all isozymes increased. Isozyme I2 showed the highest enhancement (30-fold), followed by I1 (8-fold), B (5.6-fold) and A (3-fold). Interruption of pregnancy by either physiological delivery or ambulatory abortion was followed by a sharp fall of the concentration of all isozymes reaching, in a few days, the control levels.

Authors examined validity of laparoscopy for early diagnosis of ovarian cancer. They studied 144 cases of patients with benign neoplasia diagnosed by clinical examination and found ten cases of ovarian cancer by laparoscopy. They point out validity of laparoscopy indicated as a present day essential technique for early diagnosis of ovarian cancer.

The Authors refer to two cases of sclerous lichen treated with medical therapy (Vit. E, topic betametasone and lavage with K-permanganato). They conclude that when there is no association with other dystrophies of the vulva defined precancerous, the medical therapy is the only one that supplies satisfactory results.

The RIA method has been used to assay the squamous cell carcinoma antigen in the serum of twenty-four patients suffering from cervical cancer. The results obtained showed expressive differences between the levels in women with metastatic relapse and in those in remission, but the SCC antigen is particularly useful in the therapeutic monitoring, since it can assess of the current treatment.

A case of bilateral ovarian Brenner Tumor of above average size with smooth surface, is reported. The Authors describe symptomatology and diagnostics, considering the uncertainty and rarity that still surround this neoplasia.

The Authors have carried out determinations of AFP in the serum and of CEA in serum and in surgically removed tissue of 67 patients with genital tract's malignant tumors. As the results obtained among values of pre and post operatory CEA and tissue concentrations are incongruous, they believe useful, within the monitoring of patients with gynecological cancer, dosing CEA in the serum and also on the surgical section trying to reduce so the minimum positive false and negative false. Finally the repeated dosage of these antigens allows to follow the local replaced or the metastasis after some month, before the symptoms are clear.