501. Presynaptic alpha 2 adrenoreceptor function in dependent rats before and after morphine withdrawal.
- Author
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Moises HC, Smith CB, Spengler RN, and Hollingsworth PJ
- Subjects
- Animals, Clonidine metabolism, Hippocampus drug effects, Hippocampus physiology, Hippocampus physiopathology, In Vitro Techniques, Male, Morphine pharmacology, Norepinephrine metabolism, Rats, Rats, Inbred Strains, Receptors, Adrenergic, beta drug effects, Morphine Dependence physiopathology, Receptors, Adrenergic, beta metabolism, Substance Withdrawal Syndrome physiopathology
- Abstract
Changes in the number of alpha 2 adrenoreceptors in the hippocampus, as measured by the specific binding of 3H-clonidine, were compared to changes in presynaptic alpha 2 adrenoreceptor function in electrically stimulated hippocampal slices. In dependent rats the specific binding of 3H-clonidine was reduced significantly. At 32 h after withdrawal binding began to return toward normal values, and at 72 h after withdrawal was significantly greater than that seen in saline-treated rats. In hippocampal slice experiments sensitivity to clonidine was significantly decreased in morphine-dependent animals and returned toward normal values after withdrawal. At 72 h after withdrawal, the sensitivity to clonidine of the presynaptic alpha 2 adrenoreceptor remained depressed although receptor density as measured in the binding studies was increased. This study suggests that the changes in the function of presynaptic alpha 2 adrenoreceptors might be important in the development of dependence upon morphine.
- Published
- 1986