610 results on '"Man He"'
Search Results
602. 3'-UTR SIRF: A database for identifying clusters of short interspersed repeats in 3' untranslated regions
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Ferenc Matthew T, Vincent John J, Benson Gary, Lim In, Andken Benjamin B, Heinrich Bianca, Jarzylo Larissa A, Man Heng-Ye, and Deshler James O
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Computer applications to medicine. Medical informatics ,R858-859.7 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Short (~5 nucleotides) interspersed repeats regulate several aspects of post-transcriptional gene expression. Previously we developed an algorithm (REPFIND) that assigns P-values to all repeated motifs in a given nucleic acid sequence and reliably identifies clusters of short CAC-containing motifs required for mRNA localization in Xenopus oocytes. Description In order to facilitate the identification of genes possessing clusters of repeats that regulate post-transcriptional aspects of gene expression in mammalian genes, we used REPFIND to create a database of all repeated motifs in the 3' untranslated regions (UTR) of genes from the Mammalian Gene Collection (MGC). The MGC database includes seven vertebrate species: human, cow, rat, mouse and three non-mammalian vertebrate species. A web-based application was developed to search this database of repeated motifs to generate species-specific lists of genes containing specific classes of repeats in their 3'-UTRs. This computational tool is called 3'-UTR SIRF (Short Interspersed Repeat Finder), and it reveals that hundreds of human genes contain an abundance of short CAC-rich and CAG-rich repeats in their 3'-UTRs that are similar to those found in mRNAs localized to the neurites of neurons. We tested four candidate mRNAs for localization in rat hippocampal neurons by in situ hybridization. Our results show that two candidate CAC-rich (Syntaxin 1B and Tubulin β4) and two candidate CAG-rich (Sec61α and Syntaxin 1A) mRNAs are localized to distal neurites, whereas two control mRNAs lacking repeated motifs in their 3'-UTR remain primarily in the cell body. Conclusion Computational data generated with 3'-UTR SIRF indicate that hundreds of mammalian genes have an abundance of short CA-containing motifs that may direct mRNA localization in neurons. In situ hybridization shows that four candidate mRNAs are localized to distal neurites of cultured hippocampal neurons. These data suggest that short CA-containing motifs may be part of a widely utilized genetic code that regulates mRNA localization in vertebrate cells. The use of 3'-UTR SIRF to search for new classes of motifs that regulate other aspects of gene expression should yield important information in future studies addressing cis-regulatory information located in 3'-UTRs.
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- 2007
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603. Controllable preparation of 2D carbon paper modified with flower-like WS2 for efficient microwave absorption.
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Hao Chen, Yuming Zhou, Meiyun Zhang, Shuangjiang Feng, Xiaohai Bu, Zewu Zhang, and Man He
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CARBON paper , *MULTIPLE scattering (Physics) , *MICROWAVES , *COMPOSITE coating , *ELECTROMAGNETIC waves - Abstract
In the practical application of microwave absorbing materials, traditional powder materials need to be mixed with the matrix to fabricate composite coatings. However, the complex preparation process of composite coatings and the uneven dispersion of powders in the matrix limit their application. To solve these problems, two-dimensional (2D) F-WS2/CP composite films were prepared by using carbon paper (CP) as a dispersion matrix and loading flower-like WS2 on its surface through a simple hydrothermal method. The morphology and microwave absorption (MA) performance of the composite films are easily regulated by adjusting the amount of reaction precursors. The combination of WS2 and CP facilitates impedance matching and improves the electromagnetic wave attenuation performance based on the synergistic effect of different loss mechanisms including multiple reflections and scattering, interfacial polarization, dipolar polarization, and conduction loss. At a low filler content (5 wt%), the maximum reflection loss (RL) of the composite film is up to -50 dB (99.999% energy absorption) at 12.5 GHz with 2.8 mm thickness. Moreover, at a relatively thin 1.8 mm thickness, its maximum RL remains -35 dB (>99.9% energy absorption). The as-prepared composite film shows excellent MA properties at a thinner thickness and lower filling content, providing inspiration for the preparation of light weight and efficient 2D thin-film microwave absorbers in the future. [ABSTRACT FROM AUTHOR]
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- 2023
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604. Theoretical Studies on Defects of Kaolinite in Clays.
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Man, He, Zhi, Fang, Ping, Jie, and Ping, Zhang
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KAOLINITE , *CLAY , *CRYSTAL defects , *ALUMINUM compounds , *BINDING energy , *IRON compounds , *CHROMIUM compounds - Abstract
Using the first-principles methods, we study the formation energetics and charge doping properties of the extrinsic substitutional defects in kaolinite. Especially, we choose Be, Mg, Ca, Fe, Cr, Mn, Cu, Zn as extrinsic defects to substitute for Al atoms. By systematically calculating the impurity formation energies and transition energy levels, we find that all group-II defects introduce the relative shallow transition energy levels in kaolinite. Among them, MgAl has the shallowest transition energy level at 0.08 eV above the valence band maximum. The transition-elemental defects FeAl, CrAl, and MnAl are found to have relative low formation energies, suggesting their easy formation in kaolinite under natural surrounding conditions. Our calculations show that the defects CuAl and ZnAl have the high formation energies and deep transition energy levels, which exclude the possibility of their formation in natural kaolinite. [ABSTRACT FROM AUTHOR]
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- 2009
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605. Estimated Glomerular Filtration Rate Increases in Chronic Hepatitis B Patients Treated With Telbivudine Monotherapy and Combination Treatment.
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Libin Jiang, Song Hu, Man He, and Deying Tian
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Background: Several studies have reported a renoprotective effect of telbivudine during the treatment of patients for chronic hepatitis B (CHB). Objectives: This longitudinal retrospective study aimed to examine the effects of telbivudine monotherapy and combination therapy (adefovir plus telbivudine) on renal function. Patients and Methods: This study included 336 Chinese CHB patients, who were selected from outpatients in Tongji Hospital. 44, 122, 66, 58, and 46 of these patients had been orally taking adefovir, telbivudine, entecavir, adefovir plus telbivudine, and adefovir plus lamivudine, respectively, for at least 24 months. Results: The estimated glomerular filtration rate (eGFR) in the telbivudine and adefovir plus telbivudine groups increased by 5.14 mL/min (P < 0.001) and 6.19 mL/min (P = 0.005), respectively. The patients taking the five drug regimens were further grouped into the following three subpopulations: those with compensated hepatic cirrhosis, those aged 50 or more years, and those with baseline eGFR values of 50 - 90 mL/min. The three subgroups that received telbivudine monotherapy exhibited eGFR increases of 6.38, 6.74, and 10.82 mL/min, respectively. The three subgroups that received combination therapy of adefovir plus telbivudine exhibited eGFR increases of 18.31, 14.73, and 16.59 mL/min, respectively (P < 0.05). The predictive factors for the change in eGFR levels over time were analyzed by means of two linear mixed effects models for the three monotherapy regimens and two combination regimens. Age, gender, and medication are predictive factors of eGFR changes. In addition, abnormal creatinine kinase (CK) levels in the telbivudine group were not correlated with eGFR changes (P = 0.992). Conclusions: These findings indicate that telbivudine, used in both monotherapy and combination therapy, improves the renal function of patients with CHB. The improvements are particularly significant in patients at high renal risk. [ABSTRACT FROM AUTHOR]
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- 2016
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606. Ion pair hollow fiber liquid-liquid-liquid microextraction combined with capillary electrophoresis-ultraviolet detection for the determination of thyroid hormones in human serum.
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Pingjing Li, Bin Hu, Man He, and Chen, Beibei
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ION pairs , *HOLLOW fibers , *LIQUID-liquid extraction , *CAPILLARY electrophoresis , *ULTRAVIOLET detectors , *THYROID hormones , *BLOOD serum analysis - Abstract
In this study, a novel, inexpensive, sensitive and selective analytical method that combines ion pair hollow fiber liquid-liquid-liquid microextraction (IP-HF-LLLME) with capillary electrophoresis-ultraviolet detection (CE-UV) was developed for the simultaneous determination of six thyroid hormones (including diiodothyronine (T2), 3,3,5-triiodo-l-thyronine (T3), 3,5,3,5-tetraiodolthyronine (T4), 3,3,5-triiodothyronine (rT3), monoiodotyrosine (MIT) and diiodotyrosine (DIT)) in human serum samples. By the addition of a low concentration of sodium dodecyl sulfate (SDS) into the donor phase as an ion pair reagent, octanol as the organic extraction solvent and 30mmol/L Na2CO3 as acceptor phase, six analytes with different polarity and water solubility were successfully extracted simultaneously using HF-LLLME. To the best of our knowledge, this is the first time that a liquid phase microextraction technique was proposed for the extraction of thyroid hormones in real samples. The CE separations were investigated in detail. When 20kV of voltage was applied, the six compounds were separated within 13min in 25mmol/L phosphate buffer (pH 2.15) containing 10% (v/v) acetonitrile and 0.5% (m/v) polyethylene glycol (PEG). Under the optimized conditions, enrichment factors (EFs) ranging from 183- to 366-fold were obtained and the limits of detection (at a signal-to-noise ratio of 3) were at sub µg/L level. The established IP-HF-LLLME-CE-UV method was successfully applied to simultaneous determination of thyroid hormones and relative compounds in human serum samples with good recoveries for the spiked samples. [ABSTRACT FROM AUTHOR]
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- 2014
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607. Retraction notice to "Dexmedetomidine exerts neuroprotective effect via the activation of the PI3K/Akt/mTOR signaling pathway in rats with traumatic brain injury" [Biomed. Pharmacother. 95 (2017) 885–893].
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Shen, Min, Wang, Shan, Wen, Xin, Han, Xin-Rui, Wang, Yong-Jian, Zhou, Xiu-Min, Zhang, Man-He, Wu, Dong-Mei, Lu, Jun, and Zheng, Yuan-Lin
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BRAIN injuries , *CELLULAR signal transduction , *DEXMEDETOMIDINE , *NEUROPROTECTIVE agents , *RATS - Published
- 2023
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608. Tanshinone IIA ameliorates non-alcoholic fatty liver disease through targeting peroxisome proliferator-activated receptor gamma and toll-like receptor 4
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Lu Huang, Wei Ding, Ming-Qiang Wang, Zheng-Gen Wang, Hong-Hui Chen, Wen Chen, Qiong Yang, Ting-Na Lu, Qing Yang, and Ji-Man He
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Medicine (General) ,R5-920 - Abstract
Objective To investigate the cellular mechanisms of action of tanshinone IIA on the fatty liver disease induced by a high-fat diet in an animal model of non-alcoholic fatty liver disease (NAFLD). Methods Adult male Sprague Dawley rats were randomized into one of three groups: regular rat diet (CON group) for 4 months; high-fat diet (HFD group) for 4 months; HFD for 2 months followed by tanshinone IIA treatment plus HFD (TAN group) for a further 2 months. A range of physical and biochemical markers of lipid accumulation and fatty liver disease were measured and compared between the groups. Results Tanshinone IIA treatment significantly reduced fat accumulation in the liver and plasma lipid levels that had been increased by HFD. The toll-like receptor (TLR4)/nuclear factor kappa B (NF-κB) signalling pathway was silenced by tanshinone IIA treatment. Tumour necrosis factor-α and interleukin-6 were reduced by tanshinone IIA. Hepatocyte apoptosis was inhibited by tanshinone IIA. Tanshinone IIA upregulated peroxisome proliferator-activated receptor gamma (PPAR-γ), which resulted in an improvement in the oxidative status. Conclusion Tanshinone IIA ameliorates NAFLD by targeting PPAR-γ and TLR4, resulting in decreased plasma lipids and oxidative stress, suggesting this strategy may form the basis of novel NAFLD therapies.
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- 2019
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609. Compound Bieshe Kang'ai inhibits proliferation and induces apoptosis in HCT116 human colorectal cancer cells.
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Sha Wan, Li Tan, Nan Lei, Yurong Shi, Man He, Meng Zhang, Lijuan Zhou, Liangwen Jin, Xianli Meng, Kui Yang, and Haibo Xu
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CHINESE medicine , *HERBAL medicine , *INHIBITION of cellular proliferation , *CANCER cell proliferation , *COLON cancer prevention , *APOPTOSIS , *MESSENGER RNA - Abstract
Purpose: To study the effect of Compound Bieshe Kang'ai (CBK) on proliferation and apoptosis in colorectal cancer cells. Methods: HCT116 colorectal cancer cells and FHs 74 Int intestinal cells were treated with CBK, followed by determination of cell proliferation with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Caspase-9 and caspase-3 activities as well as protein expressions of Bcl-2 and BAX, and mRNA levels of caspase-9, caspase-3, Bcl-2 and BAX in HCT116 cells were evaluated, followed by examination of the morphological alterations of HCT116 cells with Hoechst 33342 staining. Results: CBK suppressed proliferation of HCT116 cells in a concentration- and time-dependent pattern, without cytotoxicity to FHs 74 Int cells. CBK also elevated caspase-9 and caspase-3 activities, mitigated protein translation of Bcl-2 and augmented that of BAX. It also enhanced mRNA transcriptions of caspase-9, caspase-3 and BAX, but decreased that of Bcl-2 in HCT116 cells in a concentrationdependent manner, as well as induced cancer cell shrinkage, nuclear fragmentation and chromatin condensation. Conclusion: The findings highlight CBK as a promising therapeutic agent for colorectal cancers, by retarding proliferation and inducing apoptosis in cancer cells. [ABSTRACT FROM AUTHOR]
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- 2018
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610. Retracted: microRNA-129-5p involved in the neuroprotective effect of dexmedetomidine on hypoxic-ischemic brain injury by targeting COL3A1 through the Wnt/β-catenin signaling pathway in neonatal rats.
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Zhou XM, Liu J, Wang Y, Zhang SL, Zhao X, Xu X, Pei J, and Zhang MH
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Our study aims to elucidate the mechanisms how microRNA-129-5p (miR-129-5p) involved in the neuroprotective effect of dexmedetomidine (DEX) on hypoxic-ischemic brain injury (HIBI) by targeting the type III procollagen gene (COL3A1) through the Wnt/β-catenin signaling pathway in neonatal rats. A total of 120 rats were obtained, among which 15 rats were selected as sham group and rest rats as model, DEX, DEX + negative control (DEX + NC), DEX + miR-129-5p mimics, DEX + miR-129-5p inhibitors, DEX + XAV-939, and DEX + miR-129-5p inhibitors + XAV-939 groups. A dual-luciferase reporter assay was performed for the target relationship between miR-129-5p and COL3A1. Weight rate and water content of cerebral hemisphere were detected. Quantitative real-time polymerase chain reaction and Western blot analysis were conducted to detect miR-129-5p expression and expressions of COL3A1, E-cadherin, T-cell factor (TCF)- 4, and β-catenin. The DEX, DEX + miR-129-5p mimics, DEX + XAV-939 groups had increased weight rate of the cerebral hemisphere, but decreased water content of left cerebral hemisphere, levels of COL3A1, β-catenin, TCF-4, and E-cadherin in the hippocampus compared with the model and DEX + miR-129-5p inhibitors groups. COL3A1 was verified as the target gene of the miR-129-5p. Compared with the DEX + NC and DEX + miR-129-5p inhibitors + XAV-939 groups, the DEX + XAV-939 and DEX + miR-129-5p mimics groups had elevated weight rate of the cerebral hemisphere, but reduced water content of left cerebral hemisphere, levels of COL3A1, β-catenin, TCF-4, and E-cadherin in the hippocampus. Our findings demonstrate that miR-129-5p improves the neuroprotective role of DEX in HIBI by targeting COL3A1 through the Wnt/β-catenin signaling pathway in neonatal rats., (© 2019 Wiley Periodicals, Inc.)
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- 2019
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