451. Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide.
- Author
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Dötsch J, Hänze J, Dittrich K, Demirakça S, Haberberger R, and Rascher W
- Subjects
- Animals, Blotting, Northern, Enzyme Inhibitors pharmacology, Immunohistochemistry, Molsidomine analogs & derivatives, Molsidomine pharmacology, Nerve Growth Factors pharmacology, Nitric Oxide Synthase metabolism, Nitroprusside pharmacology, PC12 Cells, Phosphodiesterase Inhibitors pharmacology, Purinones pharmacology, RNA biosynthesis, RNA isolation & purification, Radioimmunoassay, Rats, Sodium-Potassium-Exchanging ATPase antagonists & inhibitors, Neuropeptide Y metabolism, Nitric Oxide physiology, Pheochromocytoma metabolism
- Abstract
Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.
- Published
- 1997
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