351. The interaction between interferon-induced protein with tetratricopeptide repeats-1 and eukaryotic elongation factor-1A.
- Author
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Li HT, Su YP, Cheng TM, Xu JM, Liao J, Chen JC, Ji CY, Ai GP, and Wang JP
- Subjects
- Adaptor Proteins, Signal Transducing, Animals, COS Cells, Carrier Proteins chemistry, Carrier Proteins genetics, Cell Death genetics, Cell Death physiology, Cells, Cultured, Chlorocebus aethiops, Mice, Models, Biological, Mutant Proteins chemistry, Mutant Proteins metabolism, Peptide Elongation Factor 1 chemistry, Peptide Elongation Factor 1 genetics, Protein Binding physiology, Protein Interaction Domains and Motifs genetics, Protein Interaction Mapping, RNA-Binding Proteins, Sequence Deletion, Tissue Distribution, Transfection, Carrier Proteins metabolism, Peptide Elongation Factor 1 metabolism
- Abstract
It has been shown previously that in mammalian cells, interferon-induced protein with tetratricopeptide repeats-1(IFIT1) is rapidly synthesized in response to viral infection, functions as an inhibitor of translation by binding to the eukaryotic initiation factor-3, and consequently assigns resistive activity against viral invasion to cells. It has also been reported that IFIT1 is rapidly produced in response to other cell stress agents with no direct relation to virus such as bacterial lipopolysaccharide and interleukin-1, but its function under these non-viral infection cell stress conditions has yet to be elucidated. Here, we demonstrate an interaction between IFIT1 and eukaryotic elongation factor-1A (eEF1A) both in vitro, using recombinant proteins as bait in pull-down assays, and in vivo, using laser confocal microscopy and immunoprecipitation. In addition, we report the initial determination of the domain of IFIT1 that mediates this interaction. We also display that both IFIT1 and eEF1A protein levels are rapidly elevated, prolonged in tumor necrosis factor alpha pre-treated Raw264.7 cells, and most of those cells are induced to death by the end of investigations. Our results imply that under some stressful stimulations IFIT1 may participate in cell death pathways by interaction with eEF1A.
- Published
- 2010
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