551. In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction.
- Author
-
de Mendoza D, Gramajo HC, and Rosa AL
- Subjects
- Bacteriophage lambda genetics, Chromosome Deletion, Genotype, Transduction, Genetic, Cloning, Molecular, Coliphages genetics, Cosmids, Escherichia coli genetics, Genes, Bacterial
- Abstract
A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in vivo into lambda phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used successfully to clone several E. coli genes.
- Published
- 1986
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