301. Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli .
- Author
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Kallas P, Valen H, Hulander M, Gadegaard N, Stormonth-Darling J, O'Reilly P, Thiede B, Andersson M, and Haugen HJ
- Subjects
- Bacteria, Bacterial Adhesion, Humans, Membrane Proteins, Surface Properties, Escherichia coli, Nanostructures
- Abstract
Developing new implant surfaces with anti-adhesion bacterial properties used for medical devices remains a challenge. Here we describe a novel study investigating nanotopography influences on bacterial adhesion on surfaces with controlled interspatial nanopillar distances. The surfaces were coated with proteins (fibrinogen, collagen, serum and saliva) prior to E. coli-WT adhesion under flow conditions. PiFM provided chemical mapping and showed that proteins adsorbed both between and onto the nanopillars with a preference for areas between the nanopillars. E. coli-WT adhered least to protein-coated areas with low surface nanopillar coverage, most to surfaces coated with saliva, while human serum led to the lowest adhesion. Protein-coated nanostructured surfaces affected the adhesion of E. coli-WT .
- Published
- 2022
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