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Background. Staphylococcus aureus bacteremia often persists and causes metastatic infections. It is unknown whether the time between blood culture incubation and growth detection (i.e., the time to positivity) in a continuously monitored system—a probable surrogate marker of bacteremia severity—correlates with outcome.Methods. We performed a prospective, observational study involving adult inpatients who had S. aureus bacteremia between 1 January 2002 and 30 June 2003 at a 600-bed teaching hospital. Measurements included time to positivity in initial blood culture series, duration of bacteremia, rate of metastatic infection, and outcome.Results. A total of 376 S. aureus bacteremias (⩾1 positive blood culture result) were reported for 357 patients aged 18–103 years (median age, 59 years); 64 bacteremias were excluded because blood was drawn after antibiotic therapy was started (n = 59) or through an intravascular catheter (n = 5). The source of infection was identified in 244 series (78.2%). Metastatic infection was detected in 25 bacteremias (8.0%). The mortality rate was 25.6%. The duration of bacteremia (determined in 251 series) was 1–59 days (median duration, 1 day; 70th percentile, 3 days). The time to positivity ranged from 4.2 to 98.2 h (median time to positivity, 15.5 h) and was significantly shorter for patients with an endovascular source of infection (14.9 ± 5.4 vs. 19.5 ± 10.6 h; P < .0005), extended duration (i.e., ⩾3 days) of bacteremia (14.1 ± 4.2 vs. 18.6 ± 9.2 h; P < .0005), and metastatic infection (12.9 ± 5.9 vs. 18.0 ± 9.3 h; P = .007). Analysis of a range of cutoff values demonstrated that a time to positivity of ⩽14 h yielded the best sensitivity and specificity for predicting the source and outcome of infection. Logistic regression analyses revealed that a time to positivity of ⩽14 h was an independent predictor of an endovascular source of infection (P < .0005), extended bacteremia (P < .0005), metastatic infection (P < .0005), and attributable mortality (P = .017).Conclusions. Time to positivity in S. aureus bacteremia may provide useful diagnostic and prognostic information. Growth of S. aureus within 14 h after the initiation of incubation may identify patients with a high likelihood of endovascular infection sources, delayed clearance, and complications.

A series of bicyclic N-substituted and unsubstituted beta-lactams were synthesized and evaluated as targeted potential antimalarials. The compounds MNR4 and MNR5 were found to have highest potency against Plasmodium falciparum in vitro.

Department of Chemistry, Kurukshetra University, Kurukshetra-136 119, India E-mail : chem@granth.kuk.ernet.in Fax : 91-01744-238277, 238628 Manuscript received 3 September 2003 Schmidt reaction of 2-benzylideneindane-1,3-diones (1) affords 2-(a-aminobenzylidene)indane-1,3-diones (2) and/or indeno[1,2- b]quinolin-11-ones (3) in fairly good yields depending upon the nature of the substituent(s) in the benzylidene phenyl ring.

Molecular analysis of the first blood culture from 41 patients with Staphylococcus aureus bacteremia and 20 bacteremia-associated catheter tip isolates revealed indistinguishable blood colonies in 39 patients (95.1%) and two related variants in two patients (4.9%). Polyclonal bacteremia was not observed in any patient, including four with genetic diversity in the catheter tip isolate.

Osteogenesis imperfecta (OI) is a disorder of type 1 collagen synthesis with varied complications in form of brittle bones, hearing loss, dental, skin, and ocular changes. Osteogenesis imperfecta may have bleeding diathesis rarely due to defective vascular integrity for the same reason. Here we come across a rare case of Sillence type IB Osteogenesis imperfecta with bleeding in the form of repeated epistaxis and petechial rash, which seem to have a collagenous link.

A 34-y-old African-American male developed Actinomyces liver abscess 8 months after appendectomy. Review of the English language literature revealed 56 additional cases of hepatic actinomycosis. Affected patients were typically immunocompetent, had a wide age range (4-86 y) and were predominantly male (70.2%). Infection was frequently (80.7%) cryptogenic, presenting with fever (83.3%), abdominal pain (74.5%) and weight loss (50.9%) over a 3.7 +/- 5.1 month period. The most common radiographic finding was a single hypodense mass/abscess (68.4%). Extension to surrounding tissues was evident in 19 cases (33.3%). Diagnosis was usually accomplished microscopically and culture was often (33.3%) negative. Infection was often (35.2%) mixed, usually with anaerobic bacteria. A surgical or percutaneous approach was diagnostic in 29/35 (82.9%) and 24/33 (72.7%) cases, respectively. The overall mortality rate was 8.8%; it was 10.7% with medical therapy alone and 4.0% using a combined medical/intervention approach (p = 0.6). In conclusion, hepatic actinomycosis is a rare subacute infection that may mimic neoplasm. It is usually cryptogenic, is more common among immunocompetent individuals and male subjects and is highly responsive to medical therapy.

A 22-y-old male developed fever, arthralgias and skin rash 2 d after tonsillectomy. Blood culture grew Neisseria meningitidis. He responded well to antibiotics. Only 1 other case of meningococcal sepsis following adenotonsillectomy has previously been reported. These cases suggest a temporal association of meningococcemia with tonsillectomy.

Investment decision making focuses on how investors make decisions to buy or sell securities. What guides their choices among alternatives, what are their investment objectives, constraints, and risk profile; and ultimately, what influences their decision making during the stock selection process. Behavioural finance deals with the psychology of the investor. It tries to explain how an investor makes an investment decision and how behavioural and other factors influence the decision making of an investor. Behavioural factors primarily include behavioural biases and personal characteristics of an investor, that is, personality, attitude, risk tolerance, and demographic factors, while other factors include external contextual factors like accounting information, market situations, brand image, and so forth, which have a bearing on decision making. The present paper provides an in-depth review of literature of prominent studies in the area of behavioural finance. The first part of the paper explains the concept of behavioral finance, the second part gives a detailed discussion on the classification of behavioural biases, and the third part discusses the effect of behavioural biases on the trading behaviour of the investors and the feelings of investors after experiencing the outcomes of investment. Towards the end, a comprehensive framework has been formulated representing the influence of behavioural biases on investment behaviour of an investor. The paper concludes that behavioural biases play a significant role in the decision making of the investors, and these biases not only shape the current investment decisions, but also influence investors' future decision making.

The muscles of shoulder girdle and elbow act together to position the hand accurately for manipulative functions. The superior extremity of man has undergone various modifications during evolution one of which is the lateral rotation of forelimbs at joints with pectoral girdle. The aim of the present study was to study the variations of pronator teres muscle. The present study was done on 60 upper limbs (56 Males and 4 Females) of embalmed adult human cadavers obtained from Deptt of Anatomy, Govt. Medical College Patiala. The humeral head of PT was found in all the cases, while Ulnar head was present in 52(86.7%) cases. The Humeral head was muscular in all the cases. The Ulnar origin was muscular in 15 cases (28.84%), tendinous in 7 cases (13.46%)&mixed in 30 cases (57.7%). In 2(3%) cases there was double humeral head and the median nerve was passing between them. In 3 cases(5%) there was high origin of humeral head of Pronator teres from medial intermuscular septum. Anatomy instructors and health professionals should be aware of the common variations in muscles and tendons of the forearm, not only for their academic interest but also for their clinical and functional implications.

Aflatoxin contamination of groundnut (Arachis hypogaea L.) is a serious problem through out the world which leads to significant economic losses every year. Invasion of groundnut seed by the aflatoxin-producing fungi Aspergillus flavus and Aspergillus parasiticus, and subsequent contamination with aflatoxins, may occur at any stage during the postharvest handling of its seeds, which necessitates development of suitable procedures for identification of the pathogen. An identification key for different fungal isolates was developed on the basis of macromorphological features viz. diameter, elevation, margins, texture of the colony as well as colour of the colony from the top and reverse of the plate, extent of sporulation (low, medium and heavy) and presence and absence of radial sulcation after seven days of culture. Microscopic features such as shape and size of conidia, vesicles and hyphae have also been included in the key. On the basis of this key different Aspergillus isolates viz. A. Niger, A. fumigatus, A flavus, A.terreus, A.parasiticus, A.oryzae could be differentiated thereby showing a potential for detection of aflatoxigenic fungi in the infected lot.

Background Fusarium oxysporum f. sp. ciceris (Foc), the causal agent of Fusarium wilt of chickpea is highly variable and frequent recurrence of virulent forms have affected chickpea production and exhausted valuable genetic resources. The severity and yield losses of Fusarium wilt differ from place to place owing to existence of physiological races among isolates. Diversity study of fungal population associated with a disease plays a major role in understanding and devising better disease control strategies. The advantages of using molecular markers to understand the distribution of genetic diversity in Foc populations is well understood. The recent development of Diversity Arrays Technology (DArT) offers new possibilities to study the diversity in pathogen population. In this study, we developed DArT markers for Foc population, analysed the genetic diversity existing within and among Foc isolates, compared the genotypic and phenotypic diversity and infer the race scenario of Foc in India. Results We report the successful development of DArT markers for Foc and their utility in genotyping of Foc collections representing five chickpea growing agro-ecological zones of India. The DArT arrays revealed a total 1,813 polymorphic markers with an average genotyping call rate of 91.16% and a scoring reproducibility of 100%. Cluster analysis, principal coordinate analysis and population structure indicated that the different isolates of Foc were partially classified based on geographical source. Diversity in Foc population was compared with the phenotypic variability and it was found that DArT markers were able to group the isolates consistent with its virulence group. A number of race-specific unique and rare alleles were also detected. Conclusion The present study generated significant information in terms of pathogenic and genetic diversity of Foc which could be used further for development and deployment of region-specific resistant cultivars of chickpea. The DArT markers were proved to be a powerful diagnostic tool to study the genotypic diversity in Foc. The high number of DArT markers allowed a greater resolution of genetic differences among isolates and enabled us to examine the extent of diversity in the Foc population present in India, as well as provided support to know the changing race scenario in Foc population. Electronic supplementary material The online version of this article (doi: 10.1186/1471-2164-15-454) contains supplementary material, which is available to authorized users.

Roots of higher plants are known to support the growth of complexes of microbes that in turn can have a profound effect on the growth and survival of the plant. Considerable amounts of dry matter produced by the plants may be released into the soil in the form of root exudates and cell sloughage (Bansal and Mukerji, 1994; Barber and Martin, 1976). Symbiotic mycorrhizal fungi are ubiquitous in occurrence and are known to colonize roots of almost all plants. Concomitant colonization and infection of roots by mycorrhizal fungi and by pathogens and other microbes inevitably lead to modification of each other’s activity (Linderman, 1985; 1989). These interactions are of great importance. Potentially beneficial interactions, if maintained or enhanced, could potentially result in biological control of pathogens.

Morphological variations of sternocleidomastoid are not uncommon. During our routine dissection, in the department of anatomy PIMS Jalandhar, we encountered a rare case of presence of third head of sternocleidomastoid, bilaterally. This anomalous head is arising from clavicle lateral to the clavicular head of sternocleidomastoid muscle. This report not only adds to the knowledge of anatomists but will hold good to surgeons, anaesthetists and radiologists as well.

Rice bran, wheat bran and gram legume powder were used as supplements in wheat straw substrate to study their effect on the production and nutritional parameters of Calocybe indica. Maximum yield (835g, 83.5%BE) was recorded in response to rice bran supplementation followed by wheat bran (790 g, 79%BE) as compared to control (575 g, 57.5% BE). The findings related to biochemical parameters showed highest protein (30.58g) in gram legume powder, highest carbohydrate (55.18g) with wheat bran and highest fat, fibre, ash and moisture (2.75g, 9.43g, 8.35g, 91.37%) respectively with rice bran. There was yield reduction when gram legume powder (470.0 g, 47% BE) was used as supplement.

The role of transfer port geometry in improving scavenging near the spark plug with consequent improvement in cyclic variations and irregular combustion have been studied through engine tests by recording the pressure-time data of many cycles with the original and modified ports on a 150cc crankcase compressed Schnurle loop scavenged two stroke engine under various operating conditions. The results show that there is an optimum upsweep angle which gives reduction in cyclic variations and lowering of delivery ratio at which irregular combustion starts. The side sweep angle does not seem to affect much. The improvement seems to be closely related to the reduction in the duration of the initial phase of combustion and its variations.

This paper presents detailed analysis of forward and reverse bias I-V and C-V characteristics of Al/Al2O3/PVA:n-ZnSe metal-oxide-semiconductor diode. PVA:n-ZnSe nanocomposites are prepared by chemical bath deposition technique. The alumina layer is deposited on Al substrate by electrolytic anodization method. The temperature dependence I-V parameters such as series resistance (RS), the ideality factor (n), the barrier height (φb), the Richardson constant (A*), mean barrier height, and the leakage current (Ileakage) have been explained on the basis of inhomogeneity. The series resistance obtained from Chenug’s method and Norde’s method shows close agreement with each other. In C-V measurements, in the reverse bias of Al/Al2O3/PVA:n-ZnSe metal-oxide-semiconductor diode has been performed as a function of temperature. The temperature dependence C-V parameters barrier height φ(C−V), the built-in-voltage (Vbi), carrier concentration (ND), Fermi energy (EF), and depletion layer width (W) have been calculated at...

Anthracene dispersed in Polyphenylsiloxane (PPS) glass was synthesized on epitaxially grown zinc oxide (ZnO) to realize organic/inorganic hybrid semiconductors for efficient energy transfer. The photoluminescence (PL) from ZnO was modified by the presence of anthracene molecules due to resonant energy transfer. The UV-visible emission from anthracene molecule was also influenced due to resonant coupling with the excitonic and defect bound excitonic states in ZnO. Temperature dependence of PL of the hybrid system showed quenching of the defect bound emission of the ZnO to be due to energy transfer from anthracene. The PL lifetime in ZnO-anthracene/PPS hybrid structure at 4 K is relatively shorter and becomes comparable to the PL lifetimes in ZnO at 77 K. However, at room temperatures the PL lifetime of the hybrid structure is significantly longer than in ZnO and is comparable to the recombination lifetime in anthracene.

This paper presents the fabrication and characterization of the Al/PVA:n-PbSe Schottky diode. I-V characteristics have been measured at different temperatures in the forward bias. The behavior study of the series resistance (RS), the ideality factor (n), the effective barrier height (Φb), the Richardson constant (A*), and the leakage current with the temperature have emphasized an inhomogeneity of the barrier height and a tunneling mechanism assisted by traps in the SBD. In C-V measurements, in the reverse bias, the Al/PVA:n-PbSe has been performed as a function of temperature and frequency. The values of barrier height (ΦC-V), the built-in–voltage (Vbi) and carrier concentration (ND) and depletion layer width (W) have been calculated at different temperatures in reverse bias. The barrier inhomogeneities of the Al/PVA:n-PbSe contact has been explained on an assumption of a Gaussian distribution of barrier heights by using the potential fluctuation model.

Methaemoglobinemia is a rare condition and can give surprise in emergency setup. We have encountered one such case in emergency recently that presented with both peripheral and central cyanosis without cardio-respiratory compromise. The patient was confirmed to be suffering from methaemoglobinemia due to antimalarials that he had received before coming to the hospital. Cyanosis due to methaemoglobinemia in our patient was precipitated by concomitant glucose 6-phosphate dehydrogenase (G6PD) deficiency. The patient was managed with conservative management and vitamin C administration.

We report a case of isolated cryptococcal myositis involving the paraspinal muscle without evidence of disseminated disease in a patient with a large B-cell lymphoma diagnosed at the time of presentation. Biopsy of the muscle involved grew a pure culture of Cryptococcus neoformans and periodic acid-Schiff staining showed numerous budding yeast consistent with Cryptococcus spp. The patient responded to systemic antifungal therapy with complete resolution of his infection. We also present a review of 5 previously published cases of cryptococcal myositis.

Calcifying epithelial odontogenic cyst is a mixed odontogenic benign tumor but most of the cases present cystic characteristics. We present a case report of calcifying epithelial odontogenic cyst with odontoma in a 20-year-old male who presented with a painless unilateral swelling of the jaw. The lesion involved an impacted 37 which was displaced to the lower border of the mandible and a calcified mass that was within the cystic lesion that was recognized as odontoma. The lesion was surgically removed along with 36 and 37 was allowed to erupt.

Botrytis grey mould (BGM), caused by Botrytis cinerea Pers. ex. Fr., is an economically important disease of chickpea (Cicer arietinum L.), especially in areas where cool, cloudy, and humid weather persists. Several epidemics of BGM causing complete crop loss in the major chickpea-producing countries have been reported. The pathogen B. cinerea mainly survives between seasons on infected crop debris and seeds. Despite extensive investigations on pathological, physiological, and molecular characteristics of B. cinerea causing grey mould type diseases on chickpea and several other hosts, the nature of infection processes and genetic basis of pathogen variability have not been clearly established. This lack of information coupled with the need for repeated application of chemical fungicides forced the deployment of host plant resistance (HPR) as a major option for BGM management. Effective and repeatable controlled-environment and field-screening techniques have been developed for identification of HPR. Of the selected portion of chickpea germplasm evaluated for BGM resistance, only few accessions belonging to both cultivated and wild Cicer spp. were tolerant to BGM, and the search for higher levels of disease resistance continues. Fungicide application based on disease predictive models is helpful in precision-based fungicide application. Integrated disease management (IDM) of BGM has proved more effective than any of the individual disease management components in large-scale, on-farm studies conducted in India, Nepal, and Bangladesh. Further information on the biology of B. cinerea and epidemiology of the disease is needed to strengthen the IDM programs. In this paper the biology of B. cinerea including its variability, epidemiology of BGM, identified sources of resistance, and other management options, and available information on biochemical and genetic basis of disease resistance have been reviewed with a mention of future research priorities.

The nutritional status of 918 preschoolers from Agra India was examined using 2 methods and standards: The Quac-stick and weight for age measures. The former sizes the circumference of the arm and is age independent; the latter is a simple measurement which considers weight as a function of age. By using the Quac-stick standards (armcircumference for height) below 85% and 80% of NCHS standards the percentage of children deemed "malnourished" was 34.75 and 8.2% respectively. The less than 80% standard using weight for age found 38.3% below that percentage. Using weight for age falling below 80% and a Quac-stick standard falling below 85% different numbers of children were counted as malnourished in the 2 methods. Using statistical sensitivity calculations it is concluded that the Quac-stick method at a less than 85% standard is a more sensitive index than the less than 80% weight for age in detecting subclinical malnutrition. The Quac-stick standard of less than 80% was not as sensitive as the less than 80% weight for age limit. Early detection of malnutrition is crucial for preventing future physical and mental complications. Sensitivity of malnutrition detection methods is therefore crucial as a preventive public health measure.

A controlled, randomized trial was conducted in 50 infants (3 to 18 months old) hospitalized with acute noncholera dehydrating diarrhea to compare the safety, efficacy, and acceptability of the standard World Health Organization (WHO) recommended glucose oral rehydration solution (ORS) (Group A: 25 infants) with that of a rice powder ORS (Group B: 25 infants), containing 30 g/L of rice powder instead of glucose (20 g/L). The electrolyte composition of both solutions was identical. The proportion of successfully treated patients in each group was 92%, and the two rehydrating solutions proved comparable in correcting and maintaining the hydration status and the serum sodium and potassium levels. The mean rehydration time, stool output, stool frequency, ORS intake, weight gain, and urine output were comparable (p greater than 0.05) in both groups. It is concluded that rice powder ORS is safe, effective, and acceptable as the standard WHO glucose ORS for the treatment of acute noncholera dehydrating diarrhea in infants.

Advances in biocontrol potentials and fungicide resistance are highly desirable for Trichoderma. Thus, it is profitable to use mutagenic agents to develop superior strains with enhanced biocontrol properties and fungicide tolerance in Trichoderma. This study investigates the N-methyl-n-nitro-N-nitrosoguanidine (NTG) (100 mg/L) induced mutants of Trichoderma asperellum. Six NTG (3 each from 1st & 2nd round) induced mutants were developed and evaluated their biocontrol activities and carbendazim tolerance. Among the mutant N2-3, N2-1, N1 and N2-2 gave the best antagonistic and volatile metabolite activities on inhibition of chickpea F. oxysporum f. sp. ciceri, B. cinerea and R. bataticola mycelium under in vitro condition. Mutant N2-2 (5626.40 μg/ml) showed the highest EC50 value against carbendazim followed by N2-3 (206.36 μg/ml) and N2-1 (16.41 μg/ml); and succeeded to sporulate even at 2000 μg/ml of carbendazim. The biocontrol activity of N2-2 and N2 with half-dose of carbendazim was evaluated on chickpea dry root rot under controlled environment. Disease reduction and progress of the dry root rot was extremely low in T7 (N2-2 + with half-dose of carbendazim) treatment. Further, carbendazim resistant mutants demonstrated mutation in tub2 gene of β-tubulin family which was suggested through the 37 and 183 residue changes in the superimposed protein structures encoded by tub2 gene in N2 and N2-2 with WT respectively. This study conclusively implies that the enhanced carbendazim tolerance in N2-2 mutant did not affect the mycoparasitism and plant growth activity of Trichoderma. These mutants were as good as the wild-type with respect to all inherent attributes.

BackgroundWith dwindling rates of postoperative mortality in perforated peptic ulcer that is attributable to H2-receptor blocker usage, there is a need to shift the focus towards the prevention of postoperative morbidity. Further, the simultaneous contribution of several putative clinical predictors to this postoperative morbidity is not fully appreciated. Our objective was to assess the predictors of the risk, rate and number of postoperative complications in surgically treated patients of perforated peptic ulcer.MethodsIn a prospective cohort study of 96 subjects presenting as perforated peptic ulcer and treated using Graham's omentoplatsy patch or gastrojejunostomy (with total truncal vagotomy), we assessed the association of clinical predictors with three domains of postoperative complications: the risk of developing a complication, the rate of developing the first complication and the risk of developing higher number of complications. We used multiple regression methods – logistic regression, Cox proportional hazards regression and Poisson regression, respectively – to examine the association of the predictors with these three domains.ResultsWe observed that the risk of developing a postoperative complication was significantly influenced by the presence of a concomitant medical illness [odds ratio (OR) = 8.9, p = 0.001], abdominal distension (3.8, 0.048) and a need of blood transfusion (OR = 8.2, p = 0.027). Using Poisson regression, it was observed that the risk for a higher number of complications was influenced by the same three factors [relative risk (RR) = 2.6, p = 0.015; RR = 4.6, p < 0.001; and RR = 2.4, p = 0.002; respectively]. However, the rate of development of complications was influenced by a history suggestive of shock [relative hazards (RH) = 3.4, p = 0.002] and A-blood group (RH = 4.7, p = 0.04).ConclusionAbdominal distension, presence of a concomitant medical illness and a history suggestive of shock at the time of admission warrant a closer and alacritous postoperative management in patients of perforated peptic ulcer.

The rapidly developing theory of complex networks indicates that real networks are not random. They have a large-scale architecture, governed by strict organizing principles. Recently, network theory has be en applied on ion track-based electronic structures such as the (TEMPOS (Tunable Electronic Materials with Pores in Oxide on Silicon) structures. In this work, the network theory has be en applied to study the surface properties of TEMPOS structures. The surface properties, i.e., number of pores and their distribution (which ultimately decide the behaviour of the TEMPOS structures) in the dielectric layer on a Si substrate, comes out to be scale-free and governed by the Power-law (power ~ 2).

Background Fusarium oxysporum f. sp. ciceris (Foc), the causal agent of Fusarium wilt is a devastating pathogen of chickpea. In chickpea, various soil borne pathogens produce (s) similar symptoms, therefore cannot be distinguished easily at field level. There is real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of Fusarium wilt outbreaks. Results In this study, we developed a loop-mediated isothermal amplification (LAMP) assay targeting the elongation factor 1 alpha gene sequence for visual detection of Foc. The LAMP reaction was optimal at 63°C for 60 min. When hydroxynaphthol blue (HNB) was added before amplification, samples with Foc DNA developed a characteristic sky blue colour but those without DNA or with the DNA of six other plant pathogenic fungi did not. Results obtained with LAMP and HNB were confirmed when LAMP products were subjected to gel electrophoresis. The detection limit of this LAMP assay for Foc was 10 fg of genomic DNA per reaction, while that of conventional PCR was 100 pg. Conclusions In conclusion, it was found that a LAMP assay combined with HNB is simple, rapid, sensitive, and specific. The LAMP assay does not require specialized equipment, hence can be used in the field for the rapid detection of Foc. This is the first report of the use of LAMP assay for the detection of Foc. The presented LAMP method provides a specific, sensitive and rapid diagnostic tool for the distinction of Foc, with the potential to be standardized as a detection method for Foc in endemic areas and will be very useful for monitoring the disease complex in the field further suggesting the management strategies. Electronic supplementary material The online version of this article (doi:10.1186/s13104-015-0997-z) contains supplementary material, which is available to authorized users.