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Background: The evidence of the relationship between sensorimotor function and blood glucose and their determinants in the elderly is limited in developing countries. This study investigated the relationship between sensorimotor function and blood glucose and compared the association between sensorimotor function and blood glucose and several determinants in Chinese people aged 50–74 years. Methods: A multistage stratified random cluster sampling method was used to sample 2476 individuals with a response rate of 81% in the Qingdao region. Pair-wise correlation analysis was performed to investigate the relationship between sensorimotor function and blood glucose. To display the association between the health domains and their determinants, t tests and one-way analysis of variance were used. Results: Near vision acuity showed a negative correlation with blood glucose (p

With the growth of big data and open data in recent years, the importance of data anonymization is increasing. Original data need to be anonymized to prevent personal identification from being revealed before being released to the public. There is a growing variety of de-identification methods which have been proposed to reduce the privacy issues, however, there is still much to be improved. The purpose of this study is to demonstrate the possibilities of re-identification from masked data, and to compare the pros and cons of different de-identification methods. A set of electronic toll collection data from Taiwan was used and we successfully re-identified vehicles with specific patterns. Four de-identification methods were performed and finally we compared the strengths and weaknesses of these methods and evaluated their appropriateness.

A fast access authentication scheme for mobile IPv6 hierarchical network was proposed, which improves the performance of access authentication of mobile IPv6 hierarchical network from two aspects, efficiency and security. Firstly, the scheme used the vector network address coding method to improve the home registration performance. Sec-ondly, a hierarchical lattice-based signature scheme was designed to implement the two-way authentication and improve the security of the authentication process. The analysis of the scheme shows that it is strongly unforgeable, and mean-while it can defend replay attacks and reduce the delay time of the entire authentication process.

A secure network coding method merged with timestamp and homomorphic signature which can solve security issues in wireless multi-hop networks was proposed.The timestamp into RSA-based homomorphic signature scheme was brought and used to produce random coefficients of net it possible to defend pollution attacks and replay attacks simultaneously while maintaining the homomorphic property of the signature.The analysis that mainly focus on was the influence of random coefficients on decoding probability of network coding a security of the proposed scheme.Results indicate that the proposed scheme can defend pollution attacks and replay attacks simultaneously,and the ratio of overhead between RSA-based homomorphic signature scheme and the proposed scheme is approximates 1.

Group awareness can affect student online learning while self-regulation also can substantially influence student online learning. Although some studies identify that these two variables may partially determine learning behavior, few empirical studies or thorough analyses elucidate the simultaneous impact of these two variables (group awareness and self-regulation) on online learning behavior. This paper compared one online collaboration environments with GA support with one without group awareness (NA) support and further investigated how these two variables, different system types (i.e., GA and NA) and different self-regulation levels (i.e., high and low), influence learning task (i.e., assessment) participation, and peer interaction (i.e., asking for help and willing to help) using two-way analysis of variance (ANOVA). Analytical results first showed that both variables have significant interaction on assessment participation and requesting rate. GA can particularly stimulate students with high-level self-regulation to engage more learning task (assessment) participation and ask for help more, compared with students with low-level self-regulation. Second, both variables have no significant interaction on willingness to help. The GA class can enhance a student’s willingness to help regardless of his/her self-regulation level.

Severe fever with thrombocytopenia syndrome virus (SFTSV) was a novel tick-borne bunyavirus that caused hemorrhagic fever with a high fatality rate in East Asia. In this study we analyzed the complete genome sequences of 122 SFTSV strains to determine the phylogeny, evolution and reassortment of the virus. We revealed that the evolutionary rate of three genome segments were different, with highest in the S segment and lowest in the L segment. The SFTSV strains were phylogenetically classified into 5 lineages (A, B, C, D and E) with each genome segment. SFTSV strains from China were classified in all 5 lineages, strains from South Korea were classified into 3 lineages (A, D, and E), and all strains from Japan were classified in only linage E. Using the average evolutionary rate of the three genome segments, we found that the extant SFTSV originated 20-87 years ago in the Dabie Mountain area in central China. The viruses were then transmitted to other areas of China, Japan and South Korea. We also found that six SFTSV strains were reassortants. Selection pressure analysis suggested that SFTSV was under purifying selection according to the four genes (RNA-dependent RNA polymerase, glycoprotein, nucleocapsid protein, non-structural protein), and two sites (37, 1033) of glycoproteins were identified as being under strong positive selection. We concluded that SFTSV originated in central China and spread to other places recently and the virus was under purifying selection with high frequency of reassortment.

A quantitative analysis has been conducted on memory contents difference for various high availability benchmarks.Based on this study memory contents patch based backup virtual machine(VM)synchronization technique was proposed,which finds the best match memory section for each dirty memory section;then compresses the difference contents in these two sections using hash based XOR compression technique in the primary VM; finally,it sends the packed data to the backup VM.For the backup VM part,it first decodes the received data and re-constructs the dirty pages,and then applies these pages to complete the VM synchronization.The experimental results show that compared with regular asynchronous replication,the proposed mechanism can reduce the synchronization data and network traffic by up to 80%,and then benefit to the synchronization to a great extent.

A non-coherent detection algorithm for CPM was proposed.Several sequential symbols were used as the observation window to calculate the prior probability for codeword in CPM soft demodulation process,and the more observation symbols were used,the better performance the algorithm achieved.Non-coherent receiver architecture was given,in which expansion method of Walsh basis function was utilized to reduce the calculating amount in matched filtering.Non-coherent detection performances of convolutional encoding CPM system adopting the proposed algorithm,with different CPM modulation parameters,were compared through simulation.The results indicate that proposed non-coherent detection algorithm brings significant robustness.As to 2CPM,4CPM serial concatenated encoding modulation,when the observation length gets to 4 symbols,system with the proposed algorithm gets nearly 6dB performance improvement than with the single-symbol non-coherent detection algorithm.

To evaluate the role of small mammals as hosts of severe fever with thrombocytopenia syndrome virus (SFTSV), we tested serum samples from rodents and shrews in China, collected in 2013. SFTSV antibodies and RNA were detected, suggesting that rodents and shrews might be hosts for SFTSV.

Since emerging in 2012, Middle East Respiratory Syndrome Coronavirus (MERS-CoV) has been a global public health threat with a high fatality rate and worldwide distribution. There are no approved vaccines or therapies for MERS until now. Passive immunotherapy with neutralizing monoclonal antibodies (mAbs) is an effective prophylactic and therapeutic reagent against emerging viruses. In this article, we review current advances in neutralizing mAbs against MERS-CoV. The receptor-binding domain (RBD) in the spike protein of MERS-CoV is a major target, and mouse, camel, or human-derived neutralizing mAbs targeting RBD have been developed. A major problem with neutralizing mAb therapy is mutant escape under selective pressure, which can be solved by combination of neutralizing mAbs targeting different epitopes. Neutralizing mAbs are currently under preclinical evaluation, and they are promising candidate therapeutic agents against MERS-CoV infection.

The unprecedented early spring frost that appears as a cold stress adversely affects growth and productivity in tea (Camellia sinensis L.); therefore, it is indispensable to develop approaches to improve the cold tolerance of tea. Here, we investigated the effect of pretreatment with exogenous melatonin on the net photosynthetic rate, the maximum photochemical efficiency of PSII, chlorophyll content, lipid peroxidation, reactive oxygen species (ROS) accumulation, antioxidant potential, and redox homeostasis in leaves of tea plants following cold stress. Our results revealed that cold treatment induced oxidative stress by increasing ROS accumulation, which in turn affected the photosynthetic process in tea leaves. However, treatment with melatonin mitigated cold-induced reductions in photosynthetic capacity by reducing oxidative stress through enhanced antioxidant potential and redox homeostasis. This study provides strong evidence that melatonin could alleviate cold-induced adverse effects in tea plants.

Graphene-based composites have gained great attention in the field of gas sensor fabrication due to their higher surface area with additional functional groups. Decorating one-dimensional (1D) semiconductor nanomaterials on graphene also show potential benefits in gas sensing applications. Here we demonstrate the one-pot and low cost synthesis of W18O49 NWs/rGO composites with different amount of reduced graphene oxide (rGO) which show excellent gas-sensing properties towards toluene and strong dependence on their chemical composition. As compared to pure W18O49 NWs, an improved gas sensing response (2.8 times higher) was achieved in case of W18O49 NWs composite with 0.5 wt. % rGO. Promisingly, this strategy can be extended to prepare other nanowire based composites with excellent gas-sensing performance.

Kiruna-type iron oxide-apatite (IOA) deposits, an important source of iron, show close associations with andesitic subvolcanic intrusions. However, the processes of ore formation and the mechanism controlling iron concentration remain uncertain. Here, we report the widespread presence of high-temperature (>800°C) water-poor multisolid hydrosaline liquid inclusions in pre-and syn-ore minerals from IOA deposits of eastern China. These inclusions consistently homogenize to a liquid phase by vapor disappearance and mostly contain 3 to 10 wt % Fe, signifying a substantial capacity for iron transportation by such hydrosaline liquids. We propose that the hydrosaline liquids were likely immiscible from the dioritic magmas with high Cl/H2O in subvolcanic settings. Subsequent reaction with host rocks and/or decompression and cooling of the hydrosaline liquids is deemed responsible for the simultaneous formation of high-temperature alteration and magnetite ores, thereby providing important insights into the distinctive characteristics of IOA deposits in shallow magmatic-hydrothermal systems. [ABSTRACT FROM AUTHOR]

BACKGROUND: Genomic aberration is a common feature of human cancers and also is one of the basic mechanisms that lead to overexpression of oncogenes and underexpression of tumor suppressor genes. Our study aims to identify frequent genomic changes in pancreatic cancer. MATERIALS AND METHODS: We used array comparative genomic hybridization (array CGH) to identify recurrent genomic alterations and validated the protein expression of selected genes by immunohistochemistry. RESULTS: Sixteen gains and thirty-two losses occurred in more than 30% and 60% of the tumors, respectively. High-level amplifications at 7q21.3-q22.1 and 19q13.2 and homozygous deletions at 1p33-p32.3, 1p22.1, 1q22, 3q27.2, 6p22.3, 6p21.31, 12q13.2, 17p13.2, 17q21.31 and 22q13.1 were identified. Especially, amplification of AKT2 was detected in two carcinomas and homozygous deletion of CDKN2C in other two cases. In 15 independent validation samples, we found that AKT2 (19q13.2) and MCM7 (7q22.1) were amplified in 6 and 9 cases, and CAMTA2 (17p13.2) and PFN1 (17p13.2) were homozygously deleted in 3 and 1 cases. AKT2 and MCM7 were overexpressed, and CAMTA2 and PFN1 were underexpressed in pancreatic cancer tissues than in morphologically normal operative margin tissues. Both GISTIC and Genomic Workbench software identified 22q13.1 containing APOBEC3A and APOBEC3B as the only homozygous deletion region. And the expression levels of APOBEC3A and APOBEC3B were significantly lower in tumor tissues than in morphologically normal operative margin tissues. Further validation showed that overexpression of PSCA was significantly associated with lymph node metastasis, and overexpression of HMGA2 was significantly associated with invasive depth of pancreatic cancer. CONCLUSION: These recurrent genomic changes may be useful for revealing the mechanism of pancreatic carcinogenesis and providing candidate biomarkers.

Two mutations have been found in five closely related insect esterases (from four higher Diptera and a hymenopteran) which each confer organophosphate (OP) hydrolase activity on the enzyme and OP resistance on the insect. One mutation converts a Glycine to an Aspartate, and the other converts a Tryptophan to a Leucine in the enzymes' active site. One of the dipteran enzymes with the Leucine mutation also shows enhanced activity against pyrethroids. Introduction of the two mutations in vitro into eight esterases from six other widely separated insect groups has also been reported to increase substantially the OP hydrolase activity of most of them. These data suggest that the two mutations could contribute to OP, and possibly pyrethroid, resistance in a variety of insects. We therefore introduced them in vitro into eight Helicoverpa armigera esterases from a clade that has already been implicated in OP and pyrethroid resistance. We found that they do not generally enhance either OP or pyrethroid hydrolysis in these esterases but the Aspartate mutation did increase OP hydrolysis in one enzyme by about 14 fold and the Leucine mutation caused a 4-6 fold increase in activity (more in one case) of another three against some of the most insecticidal isomers of fenvalerate and cypermethrin. The Aspartate enzyme and one of the Leucine enzymes occur in regions of the H. armigera esterase isozyme profile that have been previously implicated in OP and pyrethroid resistance, respectively.

Several large high-grade iron skarn deposits in the Laiwu district of Shandong Province (North China Craton) are developed along the contact zone between the Early Cretaceous Kuangshan diorite pluton and Middle Ordovician dolomitic limestone. In sharp contrast, no iron skarn mineralization has been recognized in association with other contemporaneous dioritic intrusions (Jiaoyu, Jinniushan, Tietonggou) in this district. The reason for this contrast remains elusive. Here we present a comparative study of the orerelated Kuangshan pluton and its barren equivalents using whole-rock and mineral (zircon, apatite) geochemistry combined with existing Sr-Nd isotope data to provide new insights into the critical controls on iron skarn mineralization and shed light on future mineral exploration. Geochemical and Sr-Nd isotope data suggest that both the ore-related and barren intrusions were derived from a common enriched lithospheric mantle source following variable degrees of fractional crystallization and crustal assimilation. Modeling based on whole-rock Sr-Nd isotopes implies that the magmas responsible for the Kuangshan and Jinniushan diorite plutons assimilated significant quantities of the Ordovician evaporite-bearing carbonates. Both the fertile and barren diorites show high wholerock Sr/Y ratios, high zircon Eu/Eu* and Eu/Eu*/Y ratios, and low zircon Dy/Yb ratios. These data, combined with high magmatic water contents (4.4-8.0 wt%) calculated from amphibole compositions, indicate that those four plutons crystallized from hydrous magmas. High whole-rock Fe2O3/FeO, and high ΔFMQ values (mostly >1; fayalitemagnetite-quartz oxygen buffers) estimated from zircon, apatite, and amphibole suggest that the magmas from which the diorite intrusions crystallized were all oxidized. Using available partitioning coefficients for Cl between apatite and magma, the parental melts of the Kuangshan and Jinniushan diorite are estimated to have had higher Cl contents (0.15-0.77 wt%) than the Jiaoyu and Tietonggou diorites (0.06-0.34 wt%). Two types of apatite from the intrusions studied are recognized based on their paragenetic relations: Type 1 apatite (Ap1) is an early-formed phase enclosed in euhedral amphibole, plagioclase, and biotite, whereas type 2 (Ap2) is a late-formed variety hosted in, or intergrown with, anhedral K-feldspar (± anhedral amphibole ± subhedral to anhedral quartz). In the fertile Kuangshan diorite, Cl and OH contents of apatite decrease, but Sr contents increase from Ap1 to Ap2. However, those parameters show reverse trends or remain constant from Ap1 to Ap2 in barren diorites. Such distinctive apatite geochemical signatures are best interpreted as reflecting more efficient fluid exsolution from the parental magma of the Kuangshan diorite than from the barren intrusions. This view is supported by the presence of abundant fluid inclusions in apatite and quartz exclusively from the mineralized intrusion, indicating its crystallization from volatile oversaturated melts. The results presented here indicate that elevated magma Cl contents and efficient separation of magmatic hydrothermal fluid from cooling magmas have been the critical factors controlling iron skarn mineralization in the Laiwu district. Mass balance constraints on Cl budget indicate that a minimum of 44-60 km3 of magma was required to form the Fe deposits associated with the Kuangshan pluton. Our preliminary results suggest that decreasing OH and Cl contents from early to late apatite in intermediate rocks can be used as an indicator for efficient fluid exsolution and consequently metal fertility of those rocks. [ABSTRACT FROM AUTHOR]

In a Universal Mobile telecommunications system IP multimedia subsystem (IMS), even when an IMS subscriber has passed the packet-switch domain authentication, the IMS subscribers identity must be confirmed by the IMS authentication again before accessing IMS services. Both the packet-switch domain and the IMS authentications are necessary for the IMS subscriber. This is referred to as a two-pass authentication. However, the packet-switch domain authentication is carried out by the authentication and key agreement (AKA) of the 3rd generation partnership projects (3GPP), called 3GPP AKA; the IMS authentication is carried out by IMS AKA. Since IMS AKA is based on 3GPP AKA, almost all of the operations are the same. It is inefficient because almost all the involved steps in the two-pass authentication are duplicated. Hence, the one-pass authentication was proposed to increase the efficiency of the IMS authentication. Unfortunately, the one-pass authentication has some security problems and loses the mutual AKA capabilities. Therefore, in this paper, an evolutionary IMS AKA (E-IMS AKA) in the IMS authentication is proposed to replace the IMS AKA. E-IMS AKA not only adheres to the security requirements of IMS AKA, but also maintains the efficiency of the one-pass authentication method. [ABSTRACT FROM AUTHOR]

A new magnetoresistive (MR) thin film with a structure of "antiferromagnetic layer/pinned soft magnetic layer/non-magnetic MgO spacer layer/sensitive NiFe layer" was designed. The barber-pole MR elements with a Wheatstone bridge circuit were fabricated using photolithographic methods. The testing results show that, in comparison to the element with a typical structure of Ta/NiFe/Ta, the fabricated MR element shows significant reduction in the Barkhausen noise and the 1/f noise and good magnetic stability while maintaining high magnetic field sensitivity. This element with improved signals can be attributed to the magnetostatic coupling between the pinned soft magnetic layer and the sensitive NiFe layer, which can act as a small stabilizing field, leading to the coherent rotation of magnetic moment in the sensitive NiFe layer. [ABSTRACT FROM AUTHOR]

Introduction The Iranian plateau is part of the Alpine-Himalayan orogenic belt, which consists of several continental fragments separated from each other by major boundary faults and/or ophiolitic suture zones (Gansser, 1981). Generally, the tectonic evolution of Iran has been controlled by the opening and closure of the Proto-Tethys, Paleo-Tethys and the Neo-Tethys during the Precambrian-Cambrian, Paleozoic and Cenozoic, respectively. The study area is located in the northwest of Iran (the Kurdistan province) and 20 km northeast of the city of Saqez. This area is a part of the northern Sanandaj-Sirjan Zone (Aghanabati, 2005). This belt is response to opening and subduction of Neo-Tethyan oceanic crust beneath the Central Iran (Alavi, 1994). During Cretaceous-Tertiary eras, numerous granitoid bodies were formed in this belt. The Saheb granitoid is one of these granitoid bodies which mainly consists of monzogranite, quartz monzonite and quartz monzodiorite. The aim of this research study is to discuss the evolution of the Late Cretaceous-Early Paleocene Saheb granitoids in the Sanandaj-Sirjan zone based on geology, petrography and geochronology results. Material and methods In this study, 70 rock samples were collected from different types of intrusive rocks from which 30 thin sections were prepared for petrographic studies. Furthermore, four samples from the granitoid bodies (quartz monzonite, quartz monzodiorite and monzogranite) were selected for U-Pb dating. Approximately 100 to 150 zircon grains were hand-picked by a binocular microscope from each sample. Cathodo-luminescence imaging and dating of zircon grains were examined at the China University Geosciences (Wuhan branch). Geochronological analysis were performed by using the (LA)-ICP-MS method at the China University Geosciences (Wuhan branch). The detailed analytical method is presented in Liu et al. (2010a, 2010b). Geology of the study area The Saheb granitoid body is located in the Sanandaj-Sirjan zone. According to the geological map of Chapan (scale: 1/100000, Kholghi khosraghi, 1999), the Precambrian to Quaternary units are exposed in the study area. The oldest units are the Kahar, Bayandor and Soltanieh Formations with Precambrian to Cambrian age. The Permian sediments, the Ruteh and Doroud Formations, include sandstone, shale and carbonate. The Jurassic units are found in the northwest of the region, and include sandstones and shale. The Cretaceous sedimentary units are located in the south of the study area. These sediments contain sandstone, limestone, silty-limestone, shale and dolomitic limestone. During Late Cretaceous-Early Paleocene era the Saheb granitoid intruded within the oldest units and caused Fe skarn type deposits in the Saheb area. The Saheb granitoid have been cut by a series of diabasic dikes. Results The Saheb granitoid consists of several intrusive bodies containing quartz monzonite, quartz monzodiorite and monzogranite. The major minerals in the quartz monzodiorite consist of plagioclase (35- 40%), quartz (15- 20%), orthoclase (20- 25%), and mafic minerals such as biotite and amphibole (10-15%) with granular texture. The quartz monzonitic rocks show granular and poikilitic textures. Plagioclase (25- 35%), quartz, orthoclase (30- 40%), biotite and amphibole (10-15%) are the main important minerals in the quartz monzonite. Plagioclase (20-25%), quartz (20-30%), orthoclase (30-40%), biotite and amphibole (15%) are the major minerals in the monzogranite. Zoning in zircon crystals from all four samples is well developed representing their magmatic origin (Hancar and Miller, 1993). Measurements of U-Pb in the Saheb granitoid zircon grains of quartz monzonite samples show their ages to be 62.03±0.56 Ma and 58.9±0.9 Ma. The age of monzogranite is 67.9±1.3 Ma and the age of quartz monzodiorite is 61.1±0.56 Ma. Generally, the age of this granitoid body indicates that the Saheb granitoid has occurred during the Cretaceous- Paleocene time. Discussion Based on field and microscopic studies, the Saheb granitoid bodies have been divided into three types of quartz-monzonite, quartz-monzodiorite and monzogranite. The field and mineralogical studies suggest that the Saheb granitoid is an I-type granitoid. The mineralogical variations in this granitoid suggest that the fractional crystallization has played an important role in differentiation of different compositional phases in the Saheb granitoid. According to the geochronological results, during Late Cretaceous to Early Paleocene, the Saheb granitoid intruded within the Permian and Cretaceous units in the magmatic-metamorphic Sanandaj-Sirjan zone. These granitoids were formed by subduction of Neo-Tethys Ocean beneath the Iranian plateau. It should be mentioned that the intrusion of these granitoids into the Permian carbonates and Cretaceous carbonate and shale caused formation of skarn type iron oxide mineralization. Acknowledgements The authors are grateful to the authorities at the University of Zanjan for their financial support. We also thank the authorities at the China University Geosciences (Wuhan branch) for their financial support to perform U-Pb zircon analysis.

Gold (Au) is largely hosted by pyrite in a variety of hydrothermal systems, but the incorporation of Au into pyrite under disequilibrium conditions remains poorly understood. We integrate synchrotron X-ray fluorescence microscopy, electron backscatter diffraction, nanoscale secondary ion mass spectrometry, and laser ablation-multicollector-inductively coupled plasma-mass spectrometry to constrain the processes that sequester Au into zoned pyrite in the hydrothermal cement of breccia ores from the world-class Daqiao orogenic Au deposit, central China. Euhedral pyrite cores with oscillatory and sector zoning, variable d34S values, and lower Au-As contents than the mantles are attributed to crystallization during oxidation of metal-depleted ore fluids with local variation in fluid conditions. The isotopically uniform colloform mantles are formed by pyrite crystallites separated by low-angle boundaries and are characterized by unusual decoupling of Au and As. Mantle formation is attributed to rapid disequilibrium precipitation from a metal-rich FeS2-supersaturated fluid. Incorporation of Au into the pyrite mantles was facilitated by abundant lattice defects produced by rapid nucleation. Gold-As-poor pyrite rims were deposited from an evolved ore fluid or other metal-depleted fluids. These results show that chemical variations recorded by fine layering within minerals can provide valuable insights into disequilibrium mass transfer and ore formation. The decoupling between Au and As in pyrite mantles indicates that As is not always a reliable proxy for Au enrichment in rapidly crystallized porous pyrite. [ABSTRACT FROM AUTHOR]

Chemical zoning in minerals records fluid-rock interaction and crystal growth kinetics via texturally complex features, the genesis of which remains a subject of debate. Here, we combined nanoscale secondary ion mass spectrometry (NanoSIMS) and atom probe tomography to better characterize trace-element zoning in a gold (Au)-rich pyrite crystal from the Daqiao epizonal orogenic Au deposit, China. Observations on the micron to atomic scale were used to recognize the multiple processes and mechanisms that created the zoning. Chemically distinct, micron-scale concentric zones of pyrite formed in response to changing fluid composition in a dynamic hydraulic fracturing environment. At a smaller scale, within an Au-rich zone, sector zones of Au, As, and Cu at the micron to sub-micron scale were controlled by the structure of the crystal surface. Micron-scale patchy distribution of Au, As, and Cu and atomic-scale transitions from homogeneous to heterogeneous "island" arsenian pyrite formed as a consequence of heteroepitaxial Stranski-Krastanov growth. Nanoscale Au oscillatory zoning is interpreted as a consequence of diffusion-limited self-organization processes at the crystal-fluid interface. The multiple scales of observation enabled us to see how kinetically driven intrinsic processes interacted with extrinsic factors (e.g., pressure decreases) to produce the complexity in mineral zoning. Nanoscale heterogeneities in Au, As, and Cu present as solid solution in pyrite suggest that interpretation of spikes on microbeam-derived depthconcentration profiles as metallic particles should be treated with caution. [ABSTRACT FROM AUTHOR]

The magmatic-hydrothermal evolution of porphyry-style mineralization in the shallow crust that is linked to magmatic processes at depth has been extensively studied using bulksample isotopic analysis combined with relative timing constraints. However, a lack of evaluation of the fluid evolution process against an absolute time frame limits further understanding of the ore-forming process. Here, we quantify the fluid evolution process within an absolute time frame for the first time by integrating new in situ oxygen isotope data from the Qulong porphyry Cu-Mo deposit (Tibet) with existing fluid inclusion data and high-precision Re-Os dates of co-precipitated hydrothermal quartz and molybdenite, respectively. We demonstrate that vein quartz records primary oxygen isotopic compositions and reached oxygen isotope equilibrium with ore-forming fluids, and therefore is an archive of the isotopic composition and source of the ore-forming fluids. The δ18Oquartz and δ18Ofluid values, in absolute time, show periodic fluctuations that indicate the presence of three intermittent pulses of magmatic fluid flux, which have been balanced by meteoric water. As such, the flux of magmatic fluid during ore formation was pulsed, rather than being continuous. The overall highest δ18Ofluid in the first pulse of mineralization, with a gradual decrease to the second and third pulses, is suggestive of a progressive reduction in the magmatic component of the hydrothermal fluids and, by inference, the mineralizing potential of the hydrothermal fluids. This view is supported by a decrease in sulfide-bearing fluid inclusions and metal grade through time. Our findings favor multiple fluid-release events from a single cooling magmatic reservoir, although multiple fluid-melt recharge events remain a competitive alternative. An additional implication is that the magmatic reservoir may have a lifespan of hundreds of thousands of years, with fluid release events occurring over tens of thousands of years. [ABSTRACT FROM AUTHOR]