301. [Expression and crystallographic studies of a fungal immunomodulatory protein LZ-8 from a medicinal fungus Ganoderma lucidum].
- Author
-
An M, Gao GF, Qi J, Li F, and Liu X
- Subjects
- Animals, Crystallography, Fungal Proteins genetics, Fungal Proteins immunology, Ganoderma genetics, Genetic Vectors genetics, Immunologic Factors genetics, Mice, Recombinant Proteins genetics, Recombinant Proteins immunology, Fungal Proteins biosynthesis, Ganoderma metabolism, Immunologic Factors biosynthesis, Recombinant Proteins biosynthesis
- Abstract
LZ-8 protein, isolated from a well known Chinese traditional medicinal fungus Ganoderma lucidum, is the first member of fungal immunomodulatory protein, members of which have been isolated from a variety of medicinal and edible mushrooms in the last two decades. The protein plays a multifunctional and important role in modulating immune system. In this report, in order to get LZ-8 protein crystals, the LZ-8 gene was expressed and purified by affinity chromatography, gel filtration chromatography and anion exchange chromatography subsequently. The protein was then crystallized using the hanging-drop vapour-diffusion method. The LZ-8 crystals were obtained and the phase information was calculated by X-ray diffraction. The resolution of LZ-8 crystals is 3.2A. This study will provide an insight into the structure of fungal immunomodulatory proteins.
- Published
- 2010