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501. Identification of catalytically important amino acid residues of Streptomyces lividans acetylxylan esterase A from carbohydrate esterase family 4.

502. The evolution from asparagine or threonine to cysteine in position 146 contributes to generation of a more efficient and stable form of muscle creatine kinase in higher vertebrates.

503. Asn183 in alpha5 is essential for oligomerisation and toxicity of the Bacillus thuringiensis Cry4Ba toxin.

504. Early phenotypic and genotypic alterations in submandibular gland oncogenesis in rats.

505. Association of mu-opioid receptor gene polymorphism A118G with alcohol dependence in a Japanese population.

506. An effector domain mutant of Arf6 implicates phospholipase D in endosomal membrane recycling.

507. Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein.

508. c-MYC Asn11Ser is associated with increased risk for familial breast cancer.

509. Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor.

510. Inhibition of hippocampal synaptic transmission by impairment of Ral function.

511. Transgenic mice expressing mutant (N279K) human tau show mutation dependent cognitive deficits without neurofibrillary tangle formation.

512. Processing of ovine PrP(ARQ)C-EGFP chimeras containing Asn138 and Cys151 polymorphisms.

513. A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study.

514. Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology.

515. Association of the lymphotoxin-alpha gene Thr26Asn polymorphism with severity of coronary atherosclerosis.

516. Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation.

517. Kinetic evidence for interaction of human immunodeficiency virus type 1 reverse transcriptase with the 3'-OH of the incoming dTTP substrate.

518. The DRY motif as a molecular switch of the human oxytocin receptor.

519. Single mutations at Asn295 and Leu305 in the cytoplasmic half of transmembrane alpha-helix domain 7 of the AT1 receptor induce promiscuous agonist specificity for angiotensin II fragments: a pseudo-constitutive activity.

520. pfmdr1 mutations contribute to quinine resistance and enhance mefloquine and artemisinin sensitivity in Plasmodium falciparum.

521. Structure of Escherichia coli UMP kinase differs from that of other nucleoside monophosphate kinases and sheds new light on enzyme regulation.

522. Novel compound heterozygous mutations in the PANK2 gene in a Chinese patient with atypical pantothenate kinase-associated neurodegeneration.

523. Using natural variation to investigate the function of individual amino acids in the sucrose-binding box of fructan:fructan 6G-fructosyltransferase (6G-FFT) in product formation.

524. The amino acid Asn136 in HIV-1 reverse transcriptase (RT) maintains efficient association of both RT subunits and enables the rational design of novel RT inhibitors.

525. Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D.

526. Genetic polymorphism and expression of a highly potent scorpion depressant toxin enable refinement of the effects on insect Na channels and illuminate the key role of Asn-58.

527. Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme.

528. Characterization of rat transient receptor potential vanilloid 1 receptors lacking the N-glycosylation site N604.

529. Engineering dihydropteroate synthase (DHPS) for efficient expression on M13 phage.

530. Quenching of chlorophyll triplet states by carotenoids in reconstituted Lhca4 subunit of peripheral light-harvesting complex of photosystem I.

531. Analysis of active site residues of botulinum neurotoxin E by mutational, functional, and structural studies: Glu335Gln is an apoenzyme.

532. Contribution of polar residues of the J-helix in the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I (Klenow fragment): Q677 regulates the removal of terminal mismatch.

533. Evolutionary and experimental analyses of inorganic phosphate transporter PiT family reveals two related signature sequences harboring highly conserved aspartic acids critical for sodium-dependent phosphate transport function of human PiT2.

534. STI 571 inhibition effect on KITAsn822Lys-mediated signal transduction cascade.

535. Functional analysis of the human variants of breast cancer resistance protein: I206L, N590Y, and D620N.

536. Adenylyl cyclase Rv0386 from Mycobacterium tuberculosis H37Rv uses a novel mode for substrate selection.

537. Biochemical characterization of recombinant acetyl xylan esterase from Aspergillus awamori expressed in Pichia pastoris: mutational analysis of catalytic residues.

538. Aromatic ring cleavage by homoprotocatechuate 2,3-dioxygenase: role of His200 in the kinetics of interconversion of reaction cycle intermediates.

539. Mechanism of the reaction catalyzed by isoaspartyl dipeptidase from Escherichia coli.

540. Point mutations at the type I Cu ligands, Cys457 and Met467, and at the putative proton donor, Asp105, in Myrothecium verrucaria bilirubin oxidase and reactions with dioxygen.

541. Aspartate 120 of Escherichia coli methylenetetrahydrofolate reductase: evidence for major roles in folate binding and catalysis and a minor role in flavin reactivity.

542. Asparagine deamidation perturbs antigen presentation on class II major histocompatibility complex molecules.

543. Improved catalytic properties of halohydrin dehalogenase by modification of the halide-binding site.

544. Asparagine, valine, and threonine in the third extracellular loop of muscarinic receptor have essential roles in the positive cooperativity of strychnine-like allosteric modulators.

545. Sauvagine analogs selective for corticotropin releasing factor 2 receptor: effect of substitutions at positions 35 and 39 on CRF2R selectivity.

546. The N137 and P140 amino acids in the p51 and the P95 amino acid in the p66 subunit of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase are instrumental to maintain catalytic activity and to design new classes of anti-HIV-1 drugs.

547. Asparagine-473 residue is important to the efficient function of human dihydrolipoamide dehydrogenase.

548. Hinge residue Ile260 of DNA polymerase beta is important for enzyme activity and fidelity.

549. Spontaneous chemical reversion of an active site mutation: deamidation of an asparagine residue replacing the catalytic aspartic acid of glutamate dehydrogenase.

550. Phenotypes of the N88S Berardinelli-Seip congenital lipodystrophy 2 mutation.

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