301. Novel peptide-dendrimer conjugates as drug carriers for targeting nonsmall cell lung cancer.
- Author
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Liu J, Liu J, Chu L, Wang Y, Duan Y, Feng L, Yang C, Wang L, and Kong D
- Subjects
- Amino Acid Sequence, Animals, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacokinetics, Carcinoma, Non-Small-Cell Lung metabolism, Cell Line, Tumor, Cell Survival drug effects, Dendrimers administration & dosage, Dendrimers pharmacokinetics, Dose-Response Relationship, Drug, Drug Carriers administration & dosage, Drug Carriers chemistry, Drug Carriers pharmacokinetics, Female, Fluorescein-5-isothiocyanate, Humans, Lung Neoplasms metabolism, Mice, Mice, Inbred BALB C, Peptide Library, Peptides administration & dosage, Peptides chemistry, Spectrophotometry, Ultraviolet, Tissue Distribution, Xenograft Model Antitumor Assays, Antineoplastic Agents administration & dosage, Carcinoma, Non-Small-Cell Lung drug therapy, Dendrimers chemistry, Drug Delivery Systems methods, Lung Neoplasms drug therapy, Peptides pharmacokinetics
- Abstract
Phage display technology has been demonstrated to be a powerful tool for screening useful ligands that are capable of specifically binding to biomarkers on the surface of tumor cells. The ligands found by this technique, such as peptides, have been successfully applied in the fields of early cancer diagnostics and chemotherapy. In this study, a novel nonsmall cell lung cancer-targeting peptide (LCTP, sequence RCPLSHSLICY) was screened in vivo using a Ph.D.-C7C(™) phage display library. In order to develop a universal tumor-targeting drug carrier, the LCTP and fluorescence-labeled molecule (FITC) were conjugated to an acetylated polyamidoamine (PAMAM) dendrimer of generation 4 (G4) to form a PAMAM-Ac-FITC-LCTP conjugate. The performance of the conjugate was first tested in vitro. In vitro results of cell experiments analyzed by flow cytometry and inverted fluorescence microscopy indicated that PAMAM-Ac-FITC-LCTP was enriched more in NCI-H460 cells than in 293T cells, and cellular uptake was both time- and dose-dependent. The tissue distribution of the conjugate in athymic mice with lung cancer xenografts was also investigated to test the targeting efficiency of PAMAM-Ac-FITC-LCTP in vivo. The results showed that LCTP can effectively facilitate the targeting of PAMAM-Ac-FITC-LCTP to nonsmall cell lung cancer cells and tumors. These results suggest that the LCTP-conjugated PAMAM dendrimer might be a promising drug carrier for targeted cancer diagnosis and treatment.
- Published
- 2010
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