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The Faraday rotation of weak linearly polarized probe light is observed as it passes through a sample of cold 87Rb atoms prepared by diffused light in an integrating sphere. The rotation angle of the probe light-polarization as functions of laser intensity, detuning and biased magnetic field is measured. A Ramsey fringe with a linewidth of 35 Hz and contrast up to 92% is demonstrated. This method has potential applications in improving the performance of atomic clocks with cold atoms. [ABSTRACT FROM AUTHOR]

Objectives: This study addresses the bioavailability challenges associated with oral nicotinamide mononucleotide (NMN) administration by introducing an innovative NMN formulation incorporated with hydroxyapatite (NMN–HAP). Methods: The NMN–HAP was developed using a wet chemical precipitation and physical adsorption method. To assess its superiority over conventional free NMN, we examined NMN, nicotinamide adenine dinucleotide (NAD+), and nicotinamide riboside (NR) levels in mouse plasma and tissues following oral administration of NMN–HAP. Key findings: NMN–HAP nanoparticles demonstrated a rod-shaped morphology, with an average size of ~50 nm, along with encapsulation efficiency and drug loading capacity exceeding 40%. In vitro, drug release results indicated that NMN–HAP exhibited significantly lower release compared with free NMN. In vivo studies showed that NMN–HAP extended circulation time, improved bioavailability compared with free NMN, and elevated plasma levels of NMN, NAD+, and NR. Moreover, NMN–HAP administration displayed tissue-specific distribution with a substantial accumulation of NMN, NAD+, and NR in the brain and liver. Conclusion: NMN–HAP represents an ideal formulation for enhancing NMN bioavailability, enabling tissue-specific delivery, and ultimately elevating in vivo NAD+ levels. Considering HAP's biocompatible nature and versatile characteristics, we anticipate that this system has significant potential for various future applications. Graphical Abstract [ABSTRACT FROM AUTHOR]

Recently, an absolute value inequalities discriminant analysis criterion with robustness and sparseness for supervised dimensionality reduction was studied. However, it obtains discriminant directions one by one through greedy search, which makes the sparseness of multiple discriminant directions unexplainable. In addition, it relaxes the original problem into a series of linear programming problems which makes it time consuming. In this paper, we construct a novel linear discriminant analysis with robustness and sparseness jointly through the $L_{1}$ -norm and $L_{2,1}$ -norm. The proposed approach obtains all the discriminant directions simultaneously, and rather than solving linear programming problems, it is solved by a more effective alternating direction method of multipliers. The effectiveness of the proposed method is supported by preliminary experimental results on two artificial datasets, some benchmark datasests and two face image datasets.

Background: Alzheimer's disease (AD), a type of neurodegeneration disease, is characterized by Aβ deposition and tangles of nerve fibers. Schisandrin is one of the main components of Fructus Schisandrae Chinensis. Researches showed that schisandrin can improve the cognitive impairment and memory of AD mice, but the specific mechanism has not been fully elucidated. Purpose: The purpose of this study is to investigate the possible mechanism of schisandrin in improving AD pathology. Methods: The Morris water maze test was executed to detect spatial learning and memory. Ultra performance liquid chromatography-Triple time of flight mass spectrometry (UPLC-Triple-TOF/MS)-based plasma lipidomics was used to study the changes of plasma lipids. Moreover, we measured the levels of protein and mRNA expression of APOE and ABCA1 in the rat brains and in BV2 microglia. Results: Our study found that schisandrin could improve learning and memory, and reduce Aβ deposition in AD rats. Furthermore, we found that schisandrin can improve plasma lipid metabolism disorders. Therefore, we hypothesized schisandrin might act via LXR and the docking results showed that schisandrin interacts with LXRβ. Further, we found schisandrin increased the protein and mRNA expression of LXR target genes APOE and ABCA1 in the brain of AD rats and in BV2 microglia. Conclusion: Our study reveals the neuroprotective effect and mechanism of schisandrin improves AD pathology by activating LXR to produce APOE and ABCA1. [ABSTRACT FROM AUTHOR]

The brightest gamma-ray burst, GRB 221009A, has spurred numerous theoretical investigations, with particular attention paid to the origins of ultrahigh-energy TeV photons during the prompt phase. However, analyzing the mechanism of radiation of photons in the ∼MeV range has been difficult because the high flux causes pileup and saturation effects in most GRB detectors. In this Letter, we present systematic modeling of the time-resolved spectra of the GRB using unsaturated data obtained from the Fermi Gamma-ray Burst Monitor (precursor) and SATech-01/GECAM-C (main emission and flare). Our approach incorporates the synchrotron radiation model, which assumes an expanding emission region with relativistic speed and a global magnetic field that decays with radius, and successfully fits such a model to the observational data. Our results indicate that the spectra of the burst are fully in accordance with a synchrotron origin from relativistic electrons accelerated at a large emission radius. The lack of thermal emission in the prompt emission spectra supports a Poynting flux–dominated jet composition.

The technique of laser cooling of atoms gives an opportunity to improve the performance of atomic clocks by using laser-cooled atoms. The most successful cold atom clock, called the atomic fountain, is now widely used as the primary frequency standard in many labs. The cold atom clock for satellite applications, however, has not been reported so far due to special requirements of space applications. Here, we report the development of an engineering model of a satellite-borne cold atom clock, which satisfied all requirements of in-orbit operation. The core of the clock’s principle is the laser cooling of atoms by diffuse laser lights inside the microwave cavity. The structure of the physics package is presented, and its main parameters are also given. The principle and design of the optical bench are described. The initial test results are presented, and the possible improvements are also discussed.

Abstract Ehlers–Danlos syndromes (EDSs) are a group of rare monogenic conditions with strong heterogeneity and can be caused by 20 genes associating with the essence of the extracellular matrix (ECM). This study enrolled three cases with various subtypes of EDS. Clinical evaluation and genetic testing with whole‐exome sequencing (WES) were performed. The clinical manifestations of all three patients were thoroughly monitored; and three de novo diagnostic variants, namely COL5A1: NM_001278074.1: c.4609‐2A>C, COL3A1: NM_000090.3: c.3554G>T(p.Gly1185Val), and COL1A1: NM_000088.3: c.545G>T(p.Gly182Val) were identified from them, respectively. The findings in this study expanded the mutation spectrum of EDS and strengthened the efficiency of WES in the differential diagnosis on disorders with overlapping phenotypes and various pathogenesis.

Objectives: Regional bladder wall thickening on noninvasive magnetic resonance (MR) images is an important sign of developing urinary bladder cancer (BCa), and precise segmentation of the tumor mass is an essential step toward noninvasive identification of the pathological stage and grade, which is of critical importance for the clinical management of patients with BCa. Methods: In this paper, we proposed a new method based on the high-throughput pixel-level features and a random forest (RF) classifier for the BCa segmentation. First, regions of interest (ROIs) including tumor and wall ROIs were used in the training set for feature extraction and segmentation model development. Then, candidate regions containing both bladder tumor and its neighboring wall tissue in the testing set were segmented. Results: Experimental results were evaluated on a retrospective database containing 56 patients postoperatively confirmed with BCa from the affiliated hospital. The Dice similarity coefficient (DSC) and average symmetric surface distance (ASSD) of the tumor regions were adopted to quantitatively assess the overall performance of this approach. The results showed that the mean DSC was 0.906 (95% confidential interval [CI]: 0.852-0.959), and the mean ASSD was 1.190 mm (95% CI: 1.727-2.449), which were higher than those of the state-of-the-art methods for tumor region separation. Conclusion: The proposed Pixel-level BCa segmentation method can achieve good performance for the accurate segmentation of BCa lesion on MR images.

ABSTRACT: The U-Th-REE-Nb (Ta)-polymetallic mineralization is generally related to either the silica-undersaturated syenites, the silica-oversaturated alkaline/peralkaline granites or igneous carbonatites. In this study, the authors report a new mineralization type, which is related to the magmatic-hydrothermal albitite (with mineral assemblage predominated by albite with volume content > 90%), as exemplified by the Chachaxiangka deposit in Qinghai Province of China. The Chachaxiangka deposit is the first albitite-related U-Th-REE-Nb deposit recognized in China and the mineralization can be divided into 3 types: the vein-type, the disseminated veinlet type and breccia type, of which the former 2 are predominant. Three mineralization stages can be identified according to the detailed mineralogical analyses, including the magmatic stage, main hydrothermal mineralization stage and post-ore stage. By comprehensive analyses of the mineralogical, major and trace element compositions, the authors suggest that the albitite vein is magmatic-hydrothermal in origin and both the magmatic evolution and overprint of the hydrothermal fluids play important roles in the formation of the albitite and related polymetallic mineralization. Phase separation between the silicate melt and carbonate/phosphate melt might take place in the magmatic stage, yet the immiscibility between the silicate melt and chloride-dominated fluids is the most important mechanism for the REE mineralization and also causes the Nb-Th re-mobilization and enrichment. The red color of the albitite aplite vein is an eye-catching prospecting mark in the field and more mineralization can be expected at depth and in the surrounding areas. The discovery of the new albitite type U-Th-REE-Nb mineralization give rise to new ideas during future U-Th-REE-Nb exploration, not only in the Qaidam-Altun belt, but also other areas across China.

Aim: In this work, we explored the role of corosolic acid (CRA) during pressure overload-induced cardiac hypertrophy. Methods and results: Cardiac hypertrophy was induced in mice by aortic banding. Four weeks post-surgery, CRA-treated mice developed blunted cardiac hypertrophy, fibrosis, and dysfunction, and showed increased LC3 II and p-AMPK expression. In line with the in vivo studies, CRA also inhibited the hypertrophic response induced by PE stimulation accompanying with increased LC3 II and p-AMPK expression. It was also found that CRA blunted cardiomyocyte hypertrophy and promoted autophagy in Angiotensin II (Ang II)-treated H9c2 cells. Moreover, to further verify whether CRA inhibits cardiac hypertrophy by the activation of autophagy, blockade of autophagy was achieved by CQ (an inhibitor of the fusion between autophagosomes and lysosomes) or 3-MA (an inhibitor of autophagosome formation). It was found that autophagy inhibition counteracts the protective effect of CRA on cardiac hypertrophy. Interestingly, AMPK knockdown with AMPKa2 siRNA-counteracted LC3 II expression increase and the hypertrophic response inhibition caused by CRA in PE-treated H9c2 cells. Conclusion: These results suggest that CRA may protect against cardiac hypertrophy through regulating AMPK-dependent autophagy. [ABSTRACT FROM AUTHOR]

Glycyrrhetinic acid (GA) had been the star anticancer lead compound and appealed to many scientists all over the world; however, its antitumor activity was not potent enough. To improve GA's cytoxicity and explore the effect of bonding mode on antitumor activity, 32 compounds including GA-OH series (GO, esters in C-3 position) and GA-NH 2 series (GN, with amide linkages in C-3 position) had been designed and synthesized. All the compounds were screened for in vitro cytotoxicity against A549, HepG2, MCF-7, Hela and MDCK cell lines. As a result, all the de-protected (without Boc group) derivatives showed much stronger cytotoxic activity than GA, and surprisingly enough, all the GN series of the compounds were more potent than GO series against various tumor cells. Among them, the compound 26 (amide linkages in C-3 position) exhibited stronger antitumor activity against A549 cell line (IC 50 = 2.109 ± 0.11 μM) than the positive drug cisplatin (IC 50 = 9.001 ± 0.37 μM). Further studies indicated that compound 26 could induce A549 apoptosis via nuclei fragmentation. The detection of apoptosis and cell cycle analysis indicated that compound 26 could induce the early apoptosis and prevent A549 cells transition from S to G2 phase. Furthermore, the structure-activity relationships were briefly discussed. Among which, current study displayed amide linkages in C-3 position could effectively enhance GA cytotoxicity, providing a new modification strategy for further study. Image 1 • Thirty-two glycyrrhetinic acid (GA) derivatives had been designed and synthesized. • Most of the compounds were more potent than GA and positive drug cisplatin. • All the GN series were more potent than GO series against various tumor cells. • Compound 26 showed the most potent antitumor activity among all derivatives. [ABSTRACT FROM AUTHOR]

In this study, clinical data of 31 patients in childhood with spontaneous intracerebral hemorrhage (SICH) were retrospectively analyzed. According to various imaging examinations (CT, MRI, CTA, MRA and DSA), 22 cases (70.97%) had definite causes, including 9 cases (29.03%) with intracranial arteriovenous malformation, 6 cases (19.35% ) with cavernous hemangioma, 3 cases (9.68% ) with hematopathy, 2 cases (6.45%) with tumor apoplexy, one case (3.23%) with intracranial aneurysm and one case (3.23% ) with moyamoya disease; 9 cases (29.03% ) had unclear causes. All cases were timely diagnosed and treated. Among all the patients, 23 cases (74.19%) were cured with good prognosis, 6 cases (19.35% ) improved, and the other 2 cases (6.45% ) died. Therefore, primary diseases should be timely treated as hematoma was removed.doi：10.3969/j.issn.1672-6731.2014.01.011

The authors are retracting this article [1] after an investigation by the Ethics Committee of the Fourth Military Medical University (Xi’an, Shaanxi, China) of the following concerns that had been raised with respect to two of the figures:

Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein that mediates both NADH-oxidizing and caspase-independent apoptosis. Further, the proapoptotic activity of AIF is located in the C-terminus of AIF, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. In the present study, we generated two truncated AIFs, AIFΔ1-480-FLAG, which is a FLAG-tagged C-terminal peptide comprising amino acids from 481 to 613, and AIF360-480 containing amino acids from 360 to 480 of AIF. We used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. AIFΔ1-480 but not AIF360-480 induces apoptosis in transfected cells. We also found that the expression of AIFΔ1-480 could initiate the release of cytochrome c from the mitochondria. The suppression of caspase-9 via siRNA blocked the proapoptotic activity of AIFΔ1-480. Therefore, AIFΔ 1-480 is sufficient for inducing caspase-9-dependent apoptotic signaling, probably by promoting the release of cytochrome c. At last, we generated a chimeric immuno-AIFΔ 1-480 protein, which comprised an HER2 antibody, a Pseudomonas exotoxin A translocation domain and AIFΔ 1-480. Human Jurkat cells transfected with the immuno-AIFΔl-480 gene could express and secrete the chimeric protein, which selectively recognize and kill HER2-overexpressing tumor cells. Our study demonstrates the feasibility of the immuno-AIFΔl-480 gene as a novel approach to treating HER2-overexpressing cancers.