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Background: The mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) encodes for a multifunctional receptor involved in lysosomal enzyme trafficking, fetal organogenesis, cytotoxic T cell-induced apoptosis and tumor suppression. The purpose of this investigation was to determine if the M6P/IGF2R tumor suppressor gene is mutated in human head and neck cancer, and if allelic loss is associated with poor patient prognosis. Methods: M6P/IGF2R loss of heterozygosity in locally advanced squamous cell carcinoma of the head and neck was assessed with six different gene-specific nucleotide polymorphisms. The patients studied were enrolled in a phase 3 trial of twice daily radiotherapy with or without concurrent chemotherapy; median follow-up for surviving patients is 76 months. Results: M6P/IGF2R was polymorphic in 64% (56/87) of patients, and 54% (30/56) of the tumors in these informative patients had loss of heterozygosity. M6P/IGF2R loss of heterozygosity was associated with a significantly reduced 5 year relapse-free survival (23% vs. 69%, p = 0.02), locoregional control (34% vs. 75%, p = 0.03) and cause specific survival (29% vs. 75%, p = 0.02) in the patients treated with radiotherapy alone. Concomitant chemotherapy resulted in a better outcome when compared to radiotherapy alone only in those patients whose tumors had M6P/ IGF2R loss of heterozygosity. Conclusions: This study provides the first evidence that M6P/IGF2R loss of heterozygosity predicts for poor therapeutic outcome in patients treated with radiotherapy alone. Our findings also indicate that head and neck cancer patients with M6P/IGF2R allelic loss benefit most from concurrent chemotherapy. [ABSTRACT FROM AUTHOR]

A novel and universal method, based on water-soluble poly(4-styrene sulfonate), was introduced into the preparation of a polymer mask. Using this mask, high-resolution, high-performance, bottom-gate, top-contact OFETs can be achieved. There is no solvent intervention in the process of manufacturing these OFETs and the mask can be recycled. [ABSTRACT FROM AUTHOR]

Complex energy conversion and energy dissipation occur in pump-turbines during the load rejection process. However, the underlying fluid mechanism is not clear. In order to solve these problems, in this study, a three-dimensional (3D) transient turbulent flow in a pump-turbine, with clearance during the load rejection process, was simulated using the method of coupling of the rigid rotor motion with flow and dynamic mesh technology. The simulated rotational speed shows good agreement with the experimental data. Most of the differences of rotational speed between simulations and experiments are very small and lower than 5%. Based on the numerical simulation, the energy conversion process, loss distribution, and flow mechanism in a pump-turbine were analyzed using the method of coupling of the entropy production analysis with the flow analysis. The results indicate that the load rejection process of a pump-turbine is an energy-dissipation process where the energy is converted among various energy forms. After load rejection, the hydraulic loss in the reverse pump process distributes primarily in the stay/guide vanes (GV), the vaneless space, and near draft tube inlet. While the hydraulic losses in the runaway process and the braking process are distributed mainly in the elbow section of the draft tube, the clearance of runner (RN), and the vaneless space, the hydraulic losses are mainly caused by viscous dissipation effects of the vortex flows, including the flow separation vortices, the shedding vortices of flow wake, the secondary flow, and the backflow. [ABSTRACT FROM AUTHOR]