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10,357 results on '"Lucia M"'

457. A lipopolysaccharide‐dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance

Author

Suzanne L. Warring, Lucia M. Malone, Jay Jayaraman, Richard A. Easingwood, Luciano A. Rigano, Rebekah A. Frampton, Sandra B. Visnovsky, Shea M. Addison, Loreto Hernandez, Andrew R. Pitman, Elena Lopez Acedo, Torsten Kleffmann, Matthew D. Templeton, Mihnea Bostina, and Peter C. Fineran

Subjects
Lipopolysaccharides, Proteome, Actinidia, Cryoelectron Microscopy, Glycosyltransferases, Pseudomonas syringae, Bacteriophages, Microbiology, Copper, Ecology, Evolution, Behavior and Systematics, Anti-Bacterial Agents, Plant Diseases
Abstract

SummaryBacterial pathogens are major causes of crop diseases, leading to significant production losses. For instance, kiwifruit canker, caused by the phytopathogen Pseudomonas syringae pv. actinidiae (Psa), has posed a global challenge to kiwifruit production. Treatment with copper and antibiotics, whilst initially effective, is leading to the rise of bacterial resistance, requiring new biocontrol approaches. Previously, we isolated a group of closely related Psa phages with biocontrol potential, which represent environmentally sustainable antimicrobials. However, their deployment as antimicrobials requires further insight into their properties and infection strategy. Here, we provide an in-depth examination of the genome of ΦPsa374-like phages and show that they use lipopolysaccharides (LPS) as their main receptor. Through proteomics and cryo-electron microscopy of ΦPsa374, we revealed the structural proteome and that this phage possess a T=9 capsid triangulation, unusual for myoviruses. Furthermore, we show that ΦPsa374 phage resistance arises in planta through mutations in a glycosyltransferase involved in LPS synthesis. Lastly, through in vitro evolution experiments we showed that phage-resistance is overcome by mutations in a tail fiber and structural protein of unknown function in ΦPsa374. This study provides new insight into the properties of ΦPsa374-like phages that informs their use as antimicrobials against Psa.Originality-Significance StatementThe rise of phytopathogen resistance to agrichemicals poses a significant threat to crop production, and requires urgent attention. The work presented here examines a phage genus, members of which utilize LPS as a receptor, and show potential as biocontrol agents of Psa. Studies in planta showed the development of Psa resistance to the phage, whilst maintaining bacterial virulence. This finding underscores the importance of examining phages in their ecological context for the informed design of phage cocktails that are effective in phytopathogen control.

Published
2022

458. Gestión de la innovatividad universitaria

Author

Dairaliz del V. Marcano G, Lucia M. Rojas H, and Solange Mosello de Perozo

Abstract

Las universidades están llamadas a ofrecer respuestas a las complejidades del mundo contemporáneo, con aportes significativos para el desarrollo de los países en términos de superación de las desigualdades, pobreza y exclusión; junto con el aprovechamiento racional de los recursos, que garanticen la supervivencia humana en sustentabilidad con el ambiente. La innovación es una de las características centrales en los perfiles de formación, con miras a incrementar la capacidad de las universidades para generar soluciones a problemas locales; es además un rasgo que potencia la empleabilidad de los egresados. En el marco de experiencias para la formación de competencias innovadoras en estudiantes universitarios, este estudio presenta los resultados de una investigación bajo el paradigma de la reflexividad, con el objetivo de representar en un modelo de gestión, las acciones adelantadas por una comisión a cargo de la promoción de la innovación en una universidad de Venezuela, durante 1998 – 2015. A partir de la recuperación de fuentes documentales, entrevistas y observaciones participantes, los miembros analizaron, cotejaron y sintetizaron la memoria histórica de la comisión, elaborando el Modelo de Gestión de la Innovatividad Universitaria. Se describen los ejes, procesos y valores que posibilitan la confluencia virtuosa de capacidades y actitudes articuladas, de organizaciones, personas y sistemas, para generar innovaciones. El modelo aporta buenas prácticas de alta aplicabilidad y transferencia para la promoción de una cultura de la innovación en las universidades.

Published
2022

459. COVID-19 quarantine in adolescents with autoimmune rheumatic diseases: mental health issues and life conditions

Author

Bianca P. Ihara, Lívia M. Lindoso, Debora N. D. Setoue, Nicolas Y. Tanigava, Alberto C. Helito, Juliana R. Simon, Vivianne S. L. Viana, Claudia A. A. Strabelli, Camilla A. A. Pedroso, Sofia M. Sieczkowska, Rosa M. R. Pereira, Nádia E. Aikawa, Katia T. Kozu, Adriana M. Elias, Izabel M. Buscatti, Bruno Gualano, Ligia B. Queiroz, Caio B. Casella, Guilherme V. Polanczyk, Clovis A. A. Silva, and Lucia M. M. A. Campos

Subjects
Sleep Wake Disorders, Adolescent, COVID-19, General Medicine, Arthritis, Juvenile, Dermatomyositis, Cross-Sectional Studies, Mental Health, Rheumatology, Quarantine, Humans, Lupus Erythematosus, Systemic, Prednisone, Female, Child, Pandemics
Abstract

To assess mental health and life conditions in adolescents with autoimmune rheumatic diseases (ARDs) and healthy controls quarantined during COVID-19 pandemic.A cross-sectional study included 155 ARD adolescents and 105 healthy controls. Online survey included self-reported strengths and difficulties questionnaire (SDQ), and a semi-structured questionnaire with demographic data, daily home and school routine, physical activities, and COVID-19 information during the pandemic.Among patients, 56% had juvenile idiopathic arthritis (JIA), 29% juvenile systemic lupus erythematosus (JSLE), and 15% juvenile dermatomyositis (JDM). No differences were found regarding sex, ethnicity, and current age between ARD patients and controls (p 0.05). Abnormal emotional SDQ (38% vs. 35%, p = 0.653) were similar in both groups. Logistic regression analyses in ARD patients demonstrated that female (OR = 2.4; 95%CI 1.0-6.0; p = 0.044) was associated with severe emotional SDQ dysfunction, whereas sleep problems were considered as a risk factor for both worse total SDQ (OR = 2.6; 95%CI 1.2-5.5; p = 0.009) and emotional SDQ scores (OR = 4.6; 95%CI 2.2-9.7; p 0.001). Comparisons between ARD patients with and without current prednisone use showed higher median scores of peer problems in the first group [3 (0-10) vs. 2 (0-7), p = 0.049], whereas similar median and frequencies between JIA, JSLE, and JDM (p 0.05).Approximately one third of JIA, JSLE, and JDM patients presented abnormal total and emotional scores of SDQ during COVID-19 quarantine. Sleep problems were the main factor associated with emotional difficulties in these ARD adolescents. The knowledge of mental health issues rates in adolescents with ARD supports the development of prevention strategies, like sleep hygiene counseling, as well as the references of the affected patients to specialized mental health services, as necessary. Key Points • One third of ARD patients presented mental health issues during COVID-19 quarantine • Sleep problems were associated with emotional difficulties. • It is necessary to warn pediatric rheumatologists about the importance of sleep hygiene counseling.

Published
2022

460. Insight into RNA–DNA primer length counting by human primosome

Author

Andrey G Baranovskiy, Alisa E Lisova, Lucia M Morstadt, Nigar D Babayeva, and Tahir H Tahirov

Subjects
DNA Replication, Genetics, Humans, RNA, DNA, DNA Primase, DNA-Directed DNA Polymerase, DNA Polymerase I, Chromosomes, DNA Primers
Abstract

The human primosome, a four-subunit complex of primase and DNA polymerase alpha (Polα), synthesizes chimeric RNA–DNA primers of a limited length for DNA polymerases delta and epsilon to initiate DNA replication on both chromosome strands. Despite recent structural insights into the action of its two catalytic centers, the mechanism of DNA synthesis termination is still unclear. Here we report results of functional and structural studies revealing how the human primosome counts RNA–DNA primer length and timely terminates DNA elongation. Using a single-turnover primer extension assay, we defined two factors that determine a mature primer length (∼35-mer): (i) a tight interaction of the C-terminal domain of the DNA primase large subunit (p58C) with the primer 5′-end, and (ii) flexible tethering of p58C and the DNA polymerase alpha catalytic core domain (p180core) to the primosome platform domain by extended linkers. The obtained data allow us to conclude that p58C is a key regulator of all steps of RNA–DNA primer synthesis. The above-described findings provide a notable insight into the mechanism of DNA synthesis termination by a eukaryotic primosome, an important process for ensuring successful primer handover to replication DNA polymerases and for maintaining genome integrity.

Published
2022

461. Modelling wintertime sea-spray aerosols under Arctic haze conditions

Author

Ioannidis, Eleftherios, primary, Law, Kathy S., additional, Raut, Jean-Christophe, additional, Marelle, Louis, additional, Onishi, Tatsuo, additional, Kirpes, Rachel M., additional, Upchurch, Lucia M., additional, Tuch, Thomas, additional, Wiedensohler, Alfred, additional, Massling, Andreas, additional, Skov, Henrik, additional, Quinn, Patricia K., additional, and Pratt, Kerri A., additional

Published
2023
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462. Seagrass Abundance Predicts Surficial Soil Organic Carbon Stocks Across the Range of Thalassia testudinum in the Western North Atlantic

Author

Fourqurean, James W., primary, Campbell, Justin E., additional, Rhoades, O. Kennedy, additional, Munson, Calvin J., additional, Krause, Johannes R., additional, Altieri, Andrew H., additional, Douglass, James G., additional, Heck, Kenneth L., additional, Paul, Valerie J., additional, Armitage, Anna R., additional, Barry, Savanna C., additional, Bethel, Enrique, additional, Christ, Lindsey, additional, Christianen, Marjolijn J. A., additional, Dodillet, Grace, additional, Dutton, Katrina, additional, Frazer, Thomas K., additional, Gaffey, Bethany M., additional, Glazner, Rachael, additional, Goeke, Janelle A., additional, Grana-Valdes, Rancel, additional, Kramer, Olivier A. A., additional, Linhardt, Samantha T., additional, Martin, Charles W., additional, López, Isis Gabriela Martínez, additional, McDonald, Ashley M., additional, Main, Vivienne A., additional, Manuel, Sarah A., additional, Marco-Méndez, Candela, additional, O’Brien, Duncan A., additional, O’Shea, Owen, additional, Patrick, Christopher J., additional, Peabody, Clare, additional, Reynolds, Laura K., additional, Rodriguez, Alex, additional, Bravo, Lucia M. Rodriguez, additional, Sang, Amanda, additional, Sawall, Yvonne, additional, Smulders, Fee O. H., additional, Thompson, Jamie E., additional, van Tussenbroek, Brigitta, additional, Wied, William L., additional, and Wilson, Sara S., additional

Published
2023
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463. CCR4‐IL2 bispecific immunotoxin is more effective than brentuximab for targeted therapy of cutaneous T‐cell lymphoma in a mouse CTCL model

Author

Wang, Zhaohui, primary, Ma, Jihong, additional, Zhang, Huiping, additional, Ramakrishna, Rashmi, additional, Mintzlaff, Danielle, additional, Mathes, David W., additional, Pomfret, Elizabeth A., additional, Lucia, M Scott, additional, Gao, Dexiang, additional, Haverkos, Bradley M., additional, and Wang, Zhirui, additional

Published
2023
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466. Investigating the characteristics and correlates of systemic inflammation after traumatic brain injury: the TBI-BraINFLAMM study

Author

Li, Lucia M, primary, Heslegrave, Amanda, additional, Soreq, Eyal, additional, Nattino, Giovanni, additional, Rosnati, Margherita, additional, Garbero, Elena, additional, Zimmerman, Karl A, additional, Graham, Neil S N, additional, Moro, Federico, additional, Novelli, Deborah, additional, Gradisek, Primoz, additional, Magnoni, Sandra, additional, Glocker, Ben, additional, Zetterberg, Henrik, additional, Bertolini, Guido, additional, and Sharp, David J, additional

Published
2023
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467. Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression

Author

Britto, Alan Messala A., primary, Siqueira, Juliana D., additional, Curty, Gislaine, additional, Goes, Livia R., additional, Policarpo, Cintia, additional, Meyrelles, Angela R., additional, Furtado, Yara, additional, Almeida, Gutemberg, additional, Giannini, Ana Lucia M., additional, Machado, Elizabeth S., additional, and Soares, Marcelo A., additional

Published
2023
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470. Structures of human primosome elongation complexes

Author

He, Qixiang, primary, Baranovskiy, Andrey G., additional, Morstadt, Lucia M., additional, Lisova, Alisa E., additional, Babayeva, Nigar D., additional, Lusk, Benjamin L., additional, Lim, Ci Ji, additional, and Tahirov, Tahir H., additional

Published
2023
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477. Data from Use of Aspirin and Statins in Relation to Inflammation in Benign Prostate Tissue in the Placebo Arm of the Prostate Cancer Prevention Trial

Author

Hurwitz, Lauren M., primary, Kulac, Ibrahim, primary, Gumuskaya, Berrak, primary, Valle, Javier A. Baena Del, primary, Benedetti, Ines, primary, Pan, Fan, primary, Liu, Jun O., primary, Marrone, Michael T., primary, Arnold, Kathryn B., primary, Goodman, Phyllis J., primary, Tangen, Catherine M., primary, Lucia, M. Scott, primary, Thompson, Ian M., primary, Drake, Charles G., primary, Isaacs, William B., primary, Nelson, William G., primary, De Marzo, Angelo M., primary, and Platz, Elizabeth A., primary

Published
2023
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479. Data from Association between Serum Phospholipid Fatty Acids and Intraprostatic Inflammation in the Placebo Arm of the Prostate Cancer Prevention Trial

Author

Nash, Sarah H., primary, Schenk, Jeannette M., primary, Kristal, Alan R., primary, Goodman, Phillis J., primary, Lucia, M. Scott, primary, Parnes, Howard L., primary, Thompson, Ian M., primary, Lippman, Scott M., primary, Song, Xiaoling, primary, Gurel, Bora, primary, De Marzo, Angelo, primary, and Platz, Elizabeth A., primary

Published
2023
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482. Data from Vitamin D Pathway and Other Related Polymorphisms and Risk of Prostate Cancer: Results from the Prostate Cancer Prevention Trial

Author

Torkko, Kathleen, primary, Till, Cathee, primary, Tangen, Catherine M., primary, Goodman, Phyllis J., primary, Song, Xiaoling, primary, Schenk, Jeannette M., primary, Lucia, M. Scott, primary, Peters, Ulrike, primary, van Bokhoven, Adrie, primary, Thompson, Ian M., primary, and Neuhouser, Marian L., primary

Published
2023
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486. Supplementary Tables 1-5 from Use of Aspirin and Statins in Relation to Inflammation in Benign Prostate Tissue in the Placebo Arm of the Prostate Cancer Prevention Trial

Author

Hurwitz, Lauren M., primary, Kulac, Ibrahim, primary, Gumuskaya, Berrak, primary, Valle, Javier A. Baena Del, primary, Benedetti, Ines, primary, Pan, Fan, primary, Liu, Jun O., primary, Marrone, Michael T., primary, Arnold, Kathryn B., primary, Goodman, Phyllis J., primary, Tangen, Catherine M., primary, Lucia, M. Scott, primary, Thompson, Ian M., primary, Drake, Charles G., primary, Isaacs, William B., primary, Nelson, William G., primary, De Marzo, Angelo M., primary, and Platz, Elizabeth A., primary

Published
2023
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487. Supplementary Data from Vitamin D Pathway and Other Related Polymorphisms and Risk of Prostate Cancer: Results from the Prostate Cancer Prevention Trial

Author

Torkko, Kathleen, primary, Till, Cathee, primary, Tangen, Catherine M., primary, Goodman, Phyllis J., primary, Song, Xiaoling, primary, Schenk, Jeannette M., primary, Lucia, M. Scott, primary, Peters, Ulrike, primary, van Bokhoven, Adrie, primary, Thompson, Ian M., primary, and Neuhouser, Marian L., primary

Published
2023
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488. Supplementary Tables 1-3 from Association between Serum Phospholipid Fatty Acids and Intraprostatic Inflammation in the Placebo Arm of the Prostate Cancer Prevention Trial

Author

Nash, Sarah H., primary, Schenk, Jeannette M., primary, Kristal, Alan R., primary, Goodman, Phillis J., primary, Lucia, M. Scott, primary, Parnes, Howard L., primary, Thompson, Ian M., primary, Lippman, Scott M., primary, Song, Xiaoling, primary, Gurel, Bora, primary, De Marzo, Angelo, primary, and Platz, Elizabeth A., primary

Published
2023
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490. Supplementary Table 1 from Prostate Cancer Detected by Methylated Gene Markers in Histopathologically Cancer-Negative Tissues from Men with Subsequent Positive Biopsies

Author

Troyer, Dean A., primary, Lucia, M. Scott, primary, de Bruïne, Adriann P., primary, Mendez-Meza, Rosario, primary, Baldewijns, Marcella M., primary, Dunscomb, Nancy, primary, Van Engeland, Manon, primary, McAskill, Theresa, primary, Bierau, Katja, primary, Louwagie, Joost, primary, and Bigley, Joseph W., primary

Published
2023
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491. Supplementary Figure S1 from Concurrent Alterations in TERT, KDM6A, and the BRCA Pathway in Bladder Cancer

Author

Nickerson, Michael L., primary, Dancik, Garrett M., primary, Im, Kate M., primary, Edwards, Michael G., primary, Turan, Sevilay, primary, Brown, Joseph, primary, Ruiz-Rodriguez, Christina, primary, Owens, Charles, primary, Costello, James C., primary, Guo, Guangwu, primary, Tsang, Shirley X., primary, Li, Yingrui, primary, Zhou, Quan, primary, Cai, Zhiming, primary, Moore, Lee E., primary, Lucia, M. Scott, primary, Dean, Michael, primary, and Theodorescu, Dan, primary

Published
2023
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492. Supplementary Methods, Tables 1 - 9 from Concurrent Alterations in TERT, KDM6A, and the BRCA Pathway in Bladder Cancer

Author

Nickerson, Michael L., primary, Dancik, Garrett M., primary, Im, Kate M., primary, Edwards, Michael G., primary, Turan, Sevilay, primary, Brown, Joseph, primary, Ruiz-Rodriguez, Christina, primary, Owens, Charles, primary, Costello, James C., primary, Guo, Guangwu, primary, Tsang, Shirley X., primary, Li, Yingrui, primary, Zhou, Quan, primary, Cai, Zhiming, primary, Moore, Lee E., primary, Lucia, M. Scott, primary, Dean, Michael, primary, and Theodorescu, Dan, primary

Published
2023
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493. Supplementary Figure Legend from Hedgehog Signaling Alters Reliance on EGF Receptor Signaling and Mediates Anti-EGFR Therapeutic Resistance in Head and Neck Cancer

Author

Keysar, Stephen B., primary, Le, Phuong N., primary, Anderson, Ryan T., primary, Morton, J. Jason, primary, Bowles, Daniel W., primary, Paylor, Jeramiah J., primary, Vogler, Brian W., primary, Thorburn, Jackie, primary, Fernandez, Pamela, primary, Glogowska, Magdalena J., primary, Takimoto, Sarah M., primary, Sehrt, Daniel B., primary, Gan, Gregory N., primary, Eagles-Soukup, Justin R., primary, Serracino, Hilary, primary, Hirsch, Fred R., primary, Lucia, M. Scott, primary, Thorburn, Andrew, primary, Song, John I., primary, Wang, Xiao-Jing, primary, and Jimeno, Antonio, primary

Published
2023
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494. Data from Hedgehog Signaling Alters Reliance on EGF Receptor Signaling and Mediates Anti-EGFR Therapeutic Resistance in Head and Neck Cancer

Author

Keysar, Stephen B., primary, Le, Phuong N., primary, Anderson, Ryan T., primary, Morton, J. Jason, primary, Bowles, Daniel W., primary, Paylor, Jeramiah J., primary, Vogler, Brian W., primary, Thorburn, Jackie, primary, Fernandez, Pamela, primary, Glogowska, Magdalena J., primary, Takimoto, Sarah M., primary, Sehrt, Daniel B., primary, Gan, Gregory N., primary, Eagles-Soukup, Justin R., primary, Serracino, Hilary, primary, Hirsch, Fred R., primary, Lucia, M. Scott, primary, Thorburn, Andrew, primary, Song, John I., primary, Wang, Xiao-Jing, primary, and Jimeno, Antonio, primary

Published
2023
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495. Supplementary Tables 1 - 2 from Hedgehog Signaling Alters Reliance on EGF Receptor Signaling and Mediates Anti-EGFR Therapeutic Resistance in Head and Neck Cancer

Author

Keysar, Stephen B., primary, Le, Phuong N., primary, Anderson, Ryan T., primary, Morton, J. Jason, primary, Bowles, Daniel W., primary, Paylor, Jeramiah J., primary, Vogler, Brian W., primary, Thorburn, Jackie, primary, Fernandez, Pamela, primary, Glogowska, Magdalena J., primary, Takimoto, Sarah M., primary, Sehrt, Daniel B., primary, Gan, Gregory N., primary, Eagles-Soukup, Justin R., primary, Serracino, Hilary, primary, Hirsch, Fred R., primary, Lucia, M. Scott, primary, Thorburn, Andrew, primary, Song, John I., primary, Wang, Xiao-Jing, primary, and Jimeno, Antonio, primary

Published
2023
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497. Supplementary Figures 1 - 9 from Hedgehog Signaling Alters Reliance on EGF Receptor Signaling and Mediates Anti-EGFR Therapeutic Resistance in Head and Neck Cancer

Author

Keysar, Stephen B., primary, Le, Phuong N., primary, Anderson, Ryan T., primary, Morton, J. Jason, primary, Bowles, Daniel W., primary, Paylor, Jeramiah J., primary, Vogler, Brian W., primary, Thorburn, Jackie, primary, Fernandez, Pamela, primary, Glogowska, Magdalena J., primary, Takimoto, Sarah M., primary, Sehrt, Daniel B., primary, Gan, Gregory N., primary, Eagles-Soukup, Justin R., primary, Serracino, Hilary, primary, Hirsch, Fred R., primary, Lucia, M. Scott, primary, Thorburn, Andrew, primary, Song, John I., primary, Wang, Xiao-Jing, primary, and Jimeno, Antonio, primary

Published
2023
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498. Antigenic mapping and functional characterization of human New World hantavirus neutralizing antibodies

Author

Engdahl, Taylor B, primary, Binshtein, Elad, primary, Brocato, Rebecca L, primary, Kuzmina, Natalia A, additional, Principe, Lucia M, additional, Kwilas, Steven A, additional, Kim, Robert K, additional, Chapman, Nathaniel S, additional, Porter, Monique S, additional, Guardado-Calvo, Pablo, additional, Rey, Félix A, additional, Handal, Laura S, additional, Diaz, Summer M, additional, Zagol-Ikapitte, Irene A, additional, Tran, Minh H, additional, McDonald, W Hayes, additional, Meiler, Jens, additional, Reidy, Joseph X, additional, Trivette, Andrew, additional, Bukreyev, Alexander, additional, Hooper, Jay W, additional, and Crowe, James E, additional

Published
2023
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500. Author response: Antigenic mapping and functional characterization of human New World hantavirus neutralizing antibodies

Author

Engdahl, Taylor B, primary, Binshtein, Elad, primary, Brocato, Rebecca L, primary, Kuzmina, Natalia A, additional, Principe, Lucia M, additional, Kwilas, Steven A, additional, Kim, Robert K, additional, Chapman, Nathaniel S, additional, Porter, Monique S, additional, Guardado-Calvo, Pablo, additional, Rey, Félix A, additional, Handal, Laura S, additional, Diaz, Summer M, additional, Zagol-Ikapitte, Irene A, additional, Tran, Minh H, additional, McDonald, W Hayes, additional, Meiler, Jens, additional, Reidy, Joseph X, additional, Trivette, Andrew, additional, Bukreyev, Alexander, additional, Hooper, Jay W, additional, and Crowe, James E, additional

Published
2023
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