Search

The branched fructooligosaccharides ABW90-1 and ABW50-1 from Achyranthes bidentata with potent antiosteoporosis activities have been synthesized for the first time. The synthetic approach highlights the following features: (1) 6- O -picoloyl-directed β-d-fructofuranosylation via a hydrogen-bond-mediated aglycone delivery strategy for the highly stereoselective constructions of β-(2 → 6)-d-fructofuranosidic linkages and β-(2 → 1)-d-fructofuranosidic linkages in the internal positions under the reaction conditions (DBDMH, -20 °C, CH 2 Cl 2 ) and (2) the reaction conditions (DBDMH, -78 °C to -35 °C, toluene) for highly stereoselective formations of β-(2 → 1)-d-fructofuranosidic linkages in the terminal positions.

Natural components of breast milk, human milk oligosaccharides (HMOs) and osteopontin (OPN) have been shown to have a variety of functional activities and are widely used in infant formulas. However, the preventive and therapeutic effects of both on influenza viruses are not known. In this study, antiviral assays using a human laryngeal carcinoma cell line (HEP-2) showed that 3'-sialyllactose (3'-SL) and OPN had the best antiviral ability with IC 50 values of 33.46 μM and 1.65 μM, respectively. 3'-SL (10 μM) and OPN (4 μM) were used in combination to achieve 75% inhibition. Further studies found that the combination of 200 μg/mL of 3'-SL with 500 μg/mL of OPN exerted the best antiviral ability. The reason for this was related to reduced levels of the cytokines TNF-α, IL-6, and iNOS in relation to mRNA expression. Plaque assay and TCID 50 assay found the same results and verified synergistic effects. Our research indicates that a combination of 3'-SL and OPN can effectively reduce inflammatory storms and exhibit anti-influenza virus effects through synergistic action., Competing Interests: Authors QX, QR, YL, KL and JL are employed by Heilongjiang Feihe Dairy Co. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Guo, Xie, Ren, Liu, Li, Li and Li.)

In order to broaden the sensing bandwidth of surface plasmon resonance (SPR) sensors, we propose and demonstrate a dual-channel SPR fiber optic sensor with wide bandwidth. The sensor is fabricated using no-core fiber (NCF), in which the film consists of a silver film and a ZnO film. The sensing characteristics are investigated by simulation and experiment. The resonance wavelength range of the SPR sensor can be significantly tuned by varying the thickness of the ZnO film. In the experiments, a dual-channel SPR sensor that can be used for simultaneous detection of temperature and refractive index was realized by cascading ZnO/Ag film with Ag film. The experimental results show that the two sensing channels are independent without crosstalk. The sensitivity of this sensor is 3512 nm/RIU in the range of 1.333 ∼ 1.385 and 4.6 nm/°C in the range of 0 ∼ 60 °C, which is better than most of the current dual-channel SPR sensors. In addition, the experimental results show that this sensor has good stability in use. The sensor proposed in this work has the advantages of a wide operating wavelength range, simple and compact structure, and high sensitivity. It has a broad application prospect in the simultaneous measurement of refractive index and temperature of liquids.

Enhanced ultraviolet-B (UV-B) radiation promotes anthocyanin biosynthesis in leaves, flowers and fruits of plants. However, the effects and underlying mechanisms of enhanced UV-B radiation on the accumulation of anthocyanins in the tubers of potatoes ( Solanum tuberosum L.) remain unclear. Herein, reciprocal grafting experiments were first conducted using colored and uncolored potatoes, demonstrating that the anthocyanins in potato tubers were synthesized in situ, and not transported from the leaves to the tubers. Furthermore, the enhanced UV-B radiation (2.5 kJ·m -2 ·d -1 ) on potato stems and leaves significantly increased the contents of total anthocyanin and monomeric pelargonidin and peonidin in the red-fleshed potato '21-1' tubers, compared to the untreated control. A comparative transcriptomic analysis showed that there were 2139 differentially expressed genes (DEGs) under UV-B treatment in comparison to the control, including 1724 up-regulated and 415 down-regulated genes. The anthocyanin-related enzymatic genes in the tubers such as PAL , C4H , 4CL , CHS , CHI , F3H , F3'5'H , ANS , UFGTs , and GSTs were up-regulated under UV-B treatment, except for a down-regulated F3'H . A known anthocyanin-related transcription factor StbHLH1 also showed a significantly higher expression level under UV-B treatment. Moreover, six differentially expressed MYB transcription factors were remarkably correlated to almost all anthocyanin-related enzymatic genes. Additionally, a DEGs enrichment analysis suggested that jasmonic acid might be a potential UV-B signaling molecule involved in the UV-B-induced tuber biosynthesis of anthocyanin. These results indicated that enhanced UV-B radiation in potato stems and leaves induced anthocyanin accumulation in the tubers by regulating the enzymatic genes and transcription factors involved in anthocyanin biosynthesis. This study provides novel insights into the mechanisms of enhanced UV-B radiation that regulate the anthocyanin biosynthesis in potato tubers.

The quality of the flavor of potatoes is a crucial determinant in the commercial success of a potato variety. Plant nutrition promotes the synthesis of amino acids, and the application of exogenous amino acids has the potential to enhance the flavor quality of potatoes. In this experiment, Dian Shu 1418 and Dian Shu 23 were used as the materials, and different amino acid foliar spray trials were designed. The free amino acid content in potato tubers is determined based on high-performance liquid chromatography, and volatile tuber flavor compounds are detected using gas chromatography-mass spectrometry. The results showed that the amino acid foliar spray effectively increased the content of glycine, methionine, and phenylalanine in the tubers, subsequently increasing the levels of 2,3-dimethyl-pyrazine and 2-ethyl-3-methyl-pyrazine, enhancing the roasted fragrance characteristics of the potatoes. The experimental results provide a reference for cultivating flavor enhancement in potato tubers.

Piezoelectric sensors are excellent damage detectors that can be applied to structural health monitoring (SHM). SHM for complex structures of aerospace vehicles working in harsh conditions is frequently required, posing challenging requirements for a sensor's flexibility, radiation hardness, and high-temperature tolerance. Here, we fabricate a flexible and lightweight Pb(Zr 0.53 Ti 0.47 )O 3 piezoelectric film on flexible KMg 3 (AlSi 3 O 10 )F 2 substrate via van der Waals (vdW) heteroepitaxy, endowing it with robust ferroelectric and piezoelectric properties under low energy-high flux protons (LE-HFPs) radiation (10 15 p/cm 2 ). More importantly, the Pb(Zr 0.53 Ti 0.47 )O 3 film sensor maintains highly stable damage monitoring sensitivity on an aluminum plate under harsh conditions of LE-HFPs radiation (10 15 p/cm 2 , flat structure), high temperature (175 °C, flat structure), and mechanical fatigue (bending 10 5 cycles under a radius of 5 mm, curved structure). All these superior qualities are suggested to result from the outstanding film crystal quality due to vdW epitaxy. The flexible and lightweight Pb(Zr 0.53 Ti 0.47 )O 3 film sensor demonstrated in this work provides an ideal candidate for real - time SHM of aerospace vehicles with flat and complex curve-like structures working in harsh aerospace environments.

Sulfur and phosphorus are important plant nutrients required for potato growth, influencing the synthesis of primary metabolites that serve as the material foundation of potato flavor quality. However, little is known about the effects of sulfur and phosphorus application on potato tuber flavor. This study experimentally compared the effects of the individual and combined application of phosphorus and sulfur fertilizers on the flavor of potato tubers. The research examined the sensory characteristics of flavor under various fertilization methods, investigated changes in the types and contents of volatile flavor compounds, and conducted analyses on flavor precursor compounds. The experimental results showed that the application of phosphorus and sulfur fertilizers, either individually or in combination significantly increased the content of linoleic acid and linolenic acid. After the combined application of phosphorus and sulfur fertilizers, the starch and the reducing sugar content also significantly increased. (E,E)-2,4-Nonadienal and Decanal are closely correlated with fatty acid content. Dimethyl sulfide and Trimethyl sulfide contents are significantly related to methionine content. This also significantly enhances the fatty taste characteristics of the tubers but weakens the potato flavor characteristics. Hence, the application of phosphorus and sulfur can affect the accumulation of primary metabolic products in tubers, thereby affecting flavor quality. Compared with the individual application of phosphorus or sulfur fertilizers, when phosphorus fertilizer is applied at 180 kg·ha -1 and sulfur fertilizer at 90 kg·ha -1 in combination, it can further enrich the roasted flavor characteristics of potatoes and maximize the enhancement of potato flavor quality. This provides valuable theoretical support for achieving high-quality agricultural development.

Background: Evidence of endovascular treatment (ET) for patients with progressive infarction of the posterior circulation exceeding 24 h is lacking., Objective: To evaluate the efficacy and safety of ET for progressive posterior circulation cerebral infarction., Methods: This retrospective study evaluated the ET for 18 patients with posterior circulation infarction caused by vertebrobasilar artery occlusion from July 2017 to November 2018. The conditions of patients worsened despite receiving intravenous thrombolysis or combination therapy with clopidogrel and aspirin. The time from the onset of cerebral infarction to puncture was >24 h. The preoperative National Institutes of Health Stroke Scale (NIHSS), modified Rankin Scale (mRS), and related risk factors of patients at 3 months were analyzed postoperatively., Results: The preoperative NIHSS score was 10.6 (IQR: 6.5), and the time from onset to puncture was 163.5 ± 144.7 h. Postoperative blood flow was modified thrombolysis in cerebral infarction (mTICI) grade 2b or above. During the follow-up period, 1 patient died of basilar artery re-occlusion and pulmonary infection, and 1 died of postoperative hyperperfusion hemorrhage, with a mortality rate of 11.1% (2/18). No recurrent ischemic events were observed in any of the 16 patients during the 3-month follow-up period. The mean mRS score was 1.3 (IQR: 2.3), and 75% patients (12/16) had an mRS score of 0-2. There were no significant differences in age, gender, clinical characteristics, and stroke subtype between patients with mRS scores ≤2 and >2., Conclusion: In patients with progressive posterior circulation cerebral infarction caused by vertebral basilar artery occlusion, ET is effective and safe even if the time from onset to puncture exceeds 24 h., (© 2023 Guangfeng Zhao et al., published by Sciendo.)

Transition metal oxides are promising candidates for the next generation of spintronic devices due to their fascinating properties that can be effectively engineered by strain, defects, and microstructure. An excellent example can be found in ferroelastic LaCoO 3 with paramagnetism in bulk. In contrast, unexpected ferromagnetism is observed in tensile-strained LaCoO 3 films, however, its origin remains controversial. Here we simultaneously reveal the formation of ordered oxygen vacancies and previously unreported long-range suppression of CoO 6 octahedral rotations throughout LaCoO 3 films. Supported by density functional theory calculations, we find that the strong modification of Co 3d-O 2p hybridization associated with the increase of both Co-O-Co bond angle and Co-O bond length weakens the crystal-field splitting and facilitates an ordered high-spin state of Co ions, inducing an emergent ferromagnetic-insulating state. Our work provides unique insights into underlying mechanisms driving the ferromagnetic-insulating state in tensile-strained ferroelastic LaCoO 3 films while suggesting potential applications toward low-power spintronic devices., (© 2023. The Author(s).)

Vascular aging contributes to adverse changes in organ function and is a significant indicator of major cardiac events. Endothelial cells (ECs) participate in aging-provoked coronary vascular pathology. Regular exercise is associated with preservation of arterial function with aging in humans. However, the molecular basis is not well understood. The present study was aimed to determine the effects of exercise on coronary endothelial senescence and whether mitochondrial clearance regulator FUN14 domain containing 1 (FUNDC1)-related mitophagy and mitochondrial homeostasis were involved. In mouse coronary arteries, FUNDC1 levels showed gradually decrease with age. Both FUNDC1 and mitophagy levels in cardiac microvascular endothelial cells (CMECs) were significantly reduced in aged mice and were rescued by exercise training. Exercise also alleviated CMECs senescence as evidenced by senescence associated β-galactosidase activity and aging markers, prevented endothelial abnormal cell migration, proliferation, and eNOS activation in CMECs from aged mice, and improved endothelium-dependent vasodilation of coronary artery, reduced myocardial neutrophil infiltration and inflammatory cytokines evoked by MI/R, restored angiogenesis and consequently alleviated MI/R injury in aging. Importantly, FUNDC1 deletion abolished the protective roles of exercise and FUNDC1 overexpression in ECs with adeno-associated virus (AAV) reversed endothelial senescence and prevented MI/R injury. Mechanistically, PPARγ played an important role in regulating FUNDC1 expressions in endothelium under exercise-induced laminar shear stress. In conclusion, exercise prevents endothelial senescence in coronary arteries via increasing FUNDC1 in a PPARγ-dependent manner, and subsequently protects aged mice against MI/R injury. These findings highlight FUNDC1-mediated mitophagy as potential therapeutic target that prevents endothelial senescence and myocardial vulnerability., Competing Interests: Declaration of competing interest None., (Copyright © 2023. Published by Elsevier B.V.)

For practical lithium-sulfur batteries (LSBs), the high sulfur loading and lean-electrolyte are necessary conditions to achieve the high energy density. However, such extreme conditions will cause serious battery performance fading, due to the uncontrolled deposition of Li 2 S and lithium dendrite growth. Herein, the tiny Co nanoparticles embedded N-doped carbon@Co 9 S 8 core-shell material (CoNC@Co 9 S 8 NC) is designed to address these challenges. The Co 9 S 8 NC-shell effectively captures lithium polysulfides (LiPSs) and electrolyte, and suppresses the lithium dendrite growth. The CoNC-core not only improves electronic conductivity, but also promotes Li + diffusion as well as accelerates Li 2 S deposition/decomposition. Consequently, the cell with CoNC@Co 9 S 8 NC modified separator delivers a high specific capacity of 700 mAh g -1 with a low-capacity decay rate of 0.035% per cycle at 1.0 C after 750 cycles under a sulfur loading of 3.2 mg cm -2 and a E/S ratio of 12 µL mg -1 , and a high initial areal capacity of 9.6 mAh cm -2 under a high sulfur loading of 8.8 mg cm -2 and a low E/S ratio of 4.5 µL mg -1 . Besides, the CoNC@Co 9 S 8 NC exhibits an ultralow overpotential fluctuation of 11 mV at a current density of 0.5 mA cm -2 after 1000 h during a continuous Li plating/striping process., (© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)

Sweet potatoes ( Ipomoea batatas ) are one of the important tuberous root crops cultivated worldwide, and thier storage roots are rich in antioxidants, such as anthocyanins. R2R3-MYB is a large gene family involved in various biological processes, including anthocyanin biosynthesis. However, few reports about the R2R3-MYB gene family of sweet potatoes have been released to date. In the present study, a total of 695 typical R2R3-MYB genes were identified in six Ipomoea species, including 131 R2R3-MYB genes in sweet potatoes. A maximum likelihood phylogenetic analysis divided these genes into 36 clades, referring to the classification of 126 R2R3-MYB proteins of Arabidopsis. Clade C25(S12) has no members in six Ipomoea species, whereas four clades (i.e., clade C21, C26, C30, and C36), including 102 members, had no members in Arabidopsis, and they were identified as Ipomoea -specific clades. The identified R2R3-MYB genes were unevenly distributed on all chromosomes in six Ipomoea species genomes, and the collinearity analysis among hexaploid I. batatas and another five diploid Ipomoea species suggested that the sweet potato genome might have undergone a larger chromosome rearrangement during the evolution process. Further analyses of gene duplication events showed that whole-genome duplication, transposed duplication, and dispersed duplication events were the primary forces driving the R2R3-MYB gene family expansion of Ipomoea plants, and these duplicated genes experienced strong purifying selection because of their Ka/Ks ratio, which is less than 1. Additionally, the genomic sequence length of 131 IbR2R3-MYBs varied from 923 bp to ~12.9 kb with a mean of ~2.6 kb, and most of them had more than three exons. The Motif 1, 2, 3, and 4 formed typical R2 and R3 domains and were identified in all IbR2R3-MYB proteins. Finally, based on multiple RNA-seq datasets, two IbR2R3-MYB genes ( IbMYB1/g17138.t1 and IbMYB113/g17108.t1 ) were relatively highly expressed in pigmented leaves and tuberous root flesh and skin, respectively; thus, they were identified to regulate tissue-specific anthocyanin accumulation in sweet potato. This study provides a basis for the evolution and function of the R2R3-MYB gene family in sweet potatoes and five other Ipomoea species.

The wide use of polyacrylamide (PAM) in enhanced oil recovery generates a large amount of polymer-bearing wastewater featuring high viscosity and difficult viscosity reduction, making the treatment of wastewater increasingly difficult. In this paper, the experimental study on reducing the viscosity of wastewater containing polyacrylamide by using the plasma generated by dielectric barrier discharge (DBD) and the synergistic effect of catalyst γ-Al 2 O 3 is carried out. The law of plasma reducing the viscosity of wastewater containing polyacrylamide is studied under the different conditions of amounts of γ-Al 2 O 3 catalyst, discharge voltages, and initial concentrations of polyacrylamide-containing wastewater. The mechanism of viscosity reduction of polyacrylamide is studied through environmental scanning electron microscope (ESEM), Fourier transform infrared (FTIR) spectrometer, and X-ray photoelectron spectroscopy (XPS). The results show that the catalytic viscosity reduction is the best when the discharge voltage is 18 kV and the discharge time is 15 min. With the increase in the input of the γ-Al 2 O 3 catalyst, the viscosity of the PAM solution decreases gradually. When the amount of γ-Al 2 O 3 is 375 mg, the shear rate changes from 0.5 1/sec to 28 1/sec, and the viscosity of the solution containing polyacrylamide changes from 434.5 mPa·s to 40.2 mPa·s. The viscosity reduction rate of the PAM solution is 90.7%. After the catalytic viscosity reduction, the functional groups of polyacrylamide do not change much. The elemental composition of the catalyst has not changed, which is still Al, C, and O., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The mechanism of potato ( Solanum tuberosum L.) thermotolerance has been the focus of intensive research for many years because plant growth and tuber yield are highly sensitive to heat stress. However, the linkage between the aerial and belowground parts of potato plants in response to high temperatures is not clear. To disentangle this issue, the aerial and belowground parts of the heat-resistant cultivar Dian187 (D187) and the heat-sensitive cultivar Qingshu 9 (Qs9) were independently exposed to high-temperature (30 °C) conditions using a special incubator. The results indicated that when the belowground plant parts were maintained at a normal temperature, the growth of the aerial plant parts was maintained even when independently exposed to heat stress. In contrast, the treatment that independently exposed the belowground plant parts to heat stress promoted premature senescence in the plant's leaves, even when the aerial plant parts were maintained at a normal temperature. When the aerial part of the plant was independently treated with heat stress, tuberization belowground was not delayed, and tuberization suppression was not as severe as when the belowground plant parts independently underwent heat stress. Heat stress on the belowground plant parts alone had virtually no damaging effects on the leaf photosynthetic system but caused distinct tuber deformation, secondary growth, and the loss of tuber skin colour. Transcriptome analysis revealed that the treatment of the belowground plant parts at 30 °C induced 3361 differentially expressed genes in the Qs9 cultivar's expanding tubers, while the D187 cultivar had only 10,148 differentially expressed genes. Conversely, when only the aerial plant parts were treated at 30 °C, there were just 807 DEGs (differentially expressed genes) in the D187 cultivar's expanding tubers compared with 6563 DEGs in the Qs9 cultivar, indicating that the two cultivars with different heat sensitivities have distinct regulatory mechanisms of tuberization when exposed to heat stress. The information provided in this study may be useful for further exploring the genes associated with high-temperature resistance in potato cultivars.

With the introduction of tertiary oil recovery technology, polymer oil drive technology has effectively improved the recovery rate of crude oil, but the resulting oilfield wastewater-containing polyacrylamide (PAM) is viscous and complex in composition, which brings difficulties to wastewater treatment. The treatment of this kind of wastewater has become an urgent problem to be solved, and the removal of PAM is the key. In this paper, a dielectric barrier discharge (DBD) co-catalyst was used to treat PAM-containing solutions to investigate the effect of different catalytic reaction systems on the degradation of PAM. The morphological changes of the PAM solution before and after the reaction were also studied by the environmental electron microscope scanner (ESEM), and the information of the functional groups in the solution before and after the reaction was studied by infrared spectroscopy analysis of the PAM solution. The degradation rate rose by 26.3% in comparison to that without discharge when AC/Mn + TiO 2 and Al 2 O 3 were combined and catalyzed at a mass ratio of 2:1 and a discharge period of 300 min. The degradation rate rose by 19.3 and 6.8%, respectively, in comparison to AC/Mn + TiO 2 and Al 2 O 3 -catalyzed alone. It demonstrates that this catalytic system has the optimum catalytic effect.

Surface Plasmon Resonance (SPR) based fiber optic curvature sensors have the advantage of being insensitive to temperature and axial strain. However, they have the disadvantage of low sensitivity and small curvature detection range. To improve the performance of SPR curvature sensors, we propose an intensity-modulated microstructured optical fiber (MOF) curvature sensor. In this sensor, two no-core fibers (NCFs) are used as input-output couplers, and MOF with silver film deposited is used as sensing arms. The light in the cladding is used to excite the SPR, and the exciting resonant valley is extremely sensitive to slight bending changes. The performance of this sensor is investigated theoretically and experimentally. Numerical results show that its cladding pattern is more favorable in the excitation of SPR effects. Experimental results show that the cladding mode of MOF is very sensitive to curvature changes, thus giving it a great advantage in bending measurements. Its sensitivity reaches 0.18 dB/m -1 , and linearity reaches 0.995 in the curvature range of 0-30 m -1 . The sensor has the advantages of high sensitivity, low temperature and axial strain crosstalk, compact structure, and easy fabrication, which make it attractive in the field of bending sensing.

There is limited evidence regarding the effects of dietary pattern and dietary quality on the risk of unhealthy weight status and related body composition in Chinese adolescence. In particular, studies using bioelectrical impedance analyzer (BIA) in these subjects are rare. The aim of this study was to evaluate the role of diet in body composition, to find a healthy dietary pattern for Chinese youth, and to promote the application of BIA among this population. A total of 498 participants aged from 18 to 22 years old were included. Dietary patterns were identified by principal components analysis. Energy-adjusted dietary inflammatory index (DII) and diet balance index (DBI) were calculated based on semi-quantitative food frequency questionnaire. Multivariate linear regression and logistic regression analysis were used to examine the relationship of dietary patterns, dietary quality with body mass index (BMI), fat mass index (FMI), fat-free mass index (FFMI), and the effect of dietary factors on BMI levels. The majority of participants with overweight and obesity had abdominal obesity, and there was 3.7% abdominal obesity in normal BMI individuals. Four dietary patterns were detected in the subjects. The pattern with the higher energy intake, which was close to the Western diet, was positively correlated with BMI (β = 0.326, p = 0.018) and FMI (β = 0.201, p = 0.043), while being negatively correlated with FFMI (β = −0.183, p = 0.021). Individuals who followed the pattern similar to the Mediterranean diet had a higher basal metabolic rate (BMR), and the highest fat free mass, soft lean mass, and skeletal muscle mass (p < 0.05) but the lowest FMI, visceral fat area (VFA), waist−hip ratio, and FMI/FFMI ratio (p < 0.05). Higher energy-adjusted DII was associated with high BMI. Higher bound score (HBS) (β = −0.018, p = 0.010) and diet quality distance (DQD) (β = −0.012, p = 0.015) were both negatively correlated with FFMI. In conclusion, fat or muscle indexes, such as BMR, FMI, and FFMI, had an important role in predicting overweight and obesity, which suggested the importance of applying BIA among Chinese college students. Students who followed healthful dietary patterns or the high-quality diet that is similar to the Mediterranean diet but not close to the Western diet were more likely to have a healthy BMI and normal body composition.

Iron is indispensable in numerous biologic processes, but abnormal iron regulation and accumulation is related to pathological processes in cardiovascular diseases. However, the underlying mechanisms still need to be further explored. Iron plays a key role in metal-catalyzed oxidative reactions that generate reactive oxygen species (ROS), which can cause oxidative stress. As the center for oxygen and iron utilization, mitochondria are vulnerable to damage from iron-induced oxidative stress and participate in processes involved in iron-related damage in cardiovascular disease, although the mechanism remains unclear. In this review, the pathological roles of iron-related oxidative stress in cardiovascular diseases are summarized, and the potential effects and mechanisms of mitochondrial iron homeostasis and dysfunction in these diseases are especially highlighted., Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this article., (Copyright © 2022 Fang Yan et al.)

Background: Acute myocardial dysfunction in patients with sepsis is attributed to oxidative stress, inflammation, and cardiomyocyte loss; however, specific drugs for its prevention are still lacking. Tetrahydrocurcumin (THC) has been proven to contribute to the prevention of various cardiovascular diseases by decreasing oxidative stress and inflammation. This study was performed to investigate the functions and mechanism of action of THC in septic cardiomyopathy., Methods: After the oral administration of THC (120 mg/kg) for 5 consecutive days, a mouse model of sepsis was established via intraperitoneal lipopolysaccharide (LPS, 10 mg/kg) injection. Following this, cardiac function was assessed, pathological section staining was performed, and inflammatory markers were detected., Results: Myocardial systolic function was severely compromised in parallel with the accumulation of reactive oxygen species and enhanced cardiomyocyte apoptosis in mice with sepsis. These adverse changes were markedly reversed in response to THC treatment in septic mice as well as in LPS-treated H9c2 cells. Mechanistically, THC inhibited the release of pro-inflammatory cytokines, including tumor necrosis factor alpha, interleukin (IL)-1β, and IL-6, by upregulating mitogen-activated protein kinase phosphatase 1, to block the phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated protein kinase (ERK). Additionally, THC enhanced the levels of antioxidant proteins, including nuclear factor-erythroid 2-related factor 2, superoxide dismutase 2, and NAD(P)H quinone oxidoreductase 1, while decreasing gp91 phox expression. Furthermore, upon THC treatment, Bcl-2 expression was significantly increased, along with a decline in Bax and cleaved caspase-3 expression, which reduced cardiomyocyte loss., Conclusion: Our findings indicate that THC exhibited protective potential against septic cardiomyopathy by reducing oxidative stress and inflammation through the regulation of JNK/ERK signaling. The findings of this study provide a basis for the further evaluation of THC as a therapeutic agent against septic cardiomyopathy., (Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.)

In this study, LiMn 2- x Ti x O 4 cathode materials were synthesized by a simple one-step hydrothermal method, and the effects of Ti doping on the sample structure and electrochemical properties were examined. The results indicated that Ti doping did not affect the spinel structure of LiMn 2 O 4 , and no other hybrid phases were produced. Furthermore, appropriate doping with Ti improved the particle uniformity of the samples. The electrochemical performance results showed that LiMn 1.97 Ti 0.03 O 4 exhibited much better cycling performance than the undoped sample. The discharge capacity of LiMn 1.97 Ti 0.03 O 4 reached 136 mAh g -1 at 25 °C at 0.2C, and the specific capacity reached 106.2 mAh g -1 after 300 cycles, with a capacity retention rate of 78.09%. Additionally, the specific capacity of LiMn 1.97 Ti 0.03 O 4 was 102.3 mAh g -1 after 100 cycles at 55 °C, with a capacity retention rate of 75.44%. The Ti-doped samples thus exhibited an impressive high-rate performance. The discharge capacity of LiMn 2 O 4 was only 31.3 mAh g -1 at 10C, while the discharge-specific capacity of LiMn 1.97 Ti 0.03 O 4 reached 73.4 mAh g -1 . Furthermore, to assess the higher Li + diffusion coefficient and lower internal resistance of the Ti-doped samples, cyclic voltammetry and impedance spectra data were obtained. Our results showed that Ti doping enhanced the crystal structure of LiMn 2 O 4 and improved Li + diffusion, resulting in significant improvements in the cycling and rate performance of Ti-doped samples., Competing Interests: The authors declare no competing financial interest., (© 2021 The Authors. Published by American Chemical Society.)

Nb-doped SrSnO 3 (SSNO) thin films were epitaxially grown on LaAlO 3 (001) single-crystal substrates using pulsed laser deposition under various oxygen pressures and substrate temperatures. The crystalline structure, electrical, and optical properties of the films were investigated in detail. X-ray diffraction results show that the cell volume of the films reduces gradually with increasing oxygen pressure while preserving the epitaxial characteristic. X-ray photoelectron spectroscopy analysis confirms the Nb 5+ oxidation state in the SSNO films. Hall-effect measurements were performed and the film prepared at 0.2 Pa with the 780 °C substrate temperature exhibits the lowest room-temperature resistivity of 31.3 mΩcm and Hall mobility of 3.31 cm 2 /Vs with a carrier concentration at 6.03 × 10 19 /cm 3 . Temperature-dependent resistivity of this sample displays metal-semiconductor transition and is explained mainly by electron-electron effects. Optical transparency of the films is more than 70% in the wavelength range from 600 to 1800 nm. The band gaps increase from 4.35 to 4.90 eV for the indirect gap and 4.82 to 5.29 eV for the direct by lowering oxygen pressure from 20 to 1 × 10 -3  Pa, which can be interpreted by Burstein-Moss effect and oxygen vacancies generated in the high vacuum.

For growing high quality epitaxial VO 2 thin films, the substrate with suitable lattice parameters is very important if considering the lattice matching. In addition, the thermal conductivity between the substrate and epitaxial film should be also considered. Interestingly, the c-plane of hexagonal 6H-SiC with high thermal conductivity has a similar lattice structure to the VO 2 (010), which enables epitaxial growth of high quality VO 2 films on 6H-SiC substrates. In the current study, we deposited VO 2 thin films directly on 6H-SiC (0001) single-crystal substrates by pulsed laser deposition (PLD) and systematically investigated the crystal structures and surface morphologies of the films as the function of growth temperature and film thickness. With optimized conditions, the obtained epitaxial VO 2 film showed pure monoclinic phase structure and excellent phase transition properties. Across the phase transition from monoclinic structure (M1) to tetragonal rutile structure (R), the VO 2 /6H-SiC (0001) film demonstrated a sharp resistance change up to five orders of magnitude and a narrow hysteresis width of only 3.3 °C.

Perovskite oxide SrSnO 3 has attracted considerable attentions recently due to its high carrier mobility and high transparency. Here, we experimentally and theoretically investigated the effects of La and Pb doping on the microstructure, band gaps, and electrical properties of SrSnO 3 epitaxial thin films. X-ray diffraction analysis showed that the in-plane lattice constants of Pb-doped SrSnO 3 (SrSn 1- x Pb x O 3 , x = 0-1, SSPO) films increased from 4.053 to 4.178 Å with the increase in Pb doping content. High-resolution transmission electron microscopy images revealed that SSPO films were coherently grown on LaAlO 3 (001) substrates. The optical band-gap values were considerably decreased gradually from 4.43 to 2.16 eV with Pb doping content while maintaining high optical transmittance in the visible wavelength range. Density functional theory calculations showed that the narrowing of band gap was attributed to a finite overlap between Pb 6s and Sn 5s orbitals around the bottom of the conduction band. As doping with 5% La in SSPO films, the electrical conductivity was improved greatly, and transport properties were investigated through temperature-dependent resistivity and Hall measurements. A lowest room-temperature resistivity of 0.5 mΩ cm and a maximum mobility of 39.9 cm 2 /Vs were observed in 5% La in the SSPO film at x = 1. Such wide-range tuning of the band gaps and excellent electrical properties of La- and Pb-doped SrSnO 3 epitaxial thin films may provide promising applications in optoelectronic devices.

Perovskite SrSnO 3 (SSO) thin films were epitaxially grown on LaAlO 3 (001) substrates by pulsed laser deposition at various oxygen pressures. X-ray diffraction was carried out to characterize the microstructure of the films, and the results showed that the unit-cell volume of the films increased gradually with lowering the growth oxygen pressures while remaining the perovskite structure. X-ray photoelectron spectroscopy results indicated that oxygen vacancies (OVs) existed in SSO thin films. Optical property measurements showed that all samples have a transmittance of more than 75% in the visible and near-infrared wavelength region. Furthermore, the band gaps of SSO films were found to increase from 4.56 to 5.21 eV with the decrease of deposition oxygen pressures calculated by linear fitting absorption edges of optical transmittance. In order to further ascertain the effect of OVs on band gaps of SSO films, the as-deposited 10 Pa film was annealed at 10 -4 Pa oxygen pressures and the band gap was found to increase by more than 1 eV. Density functional theory was used to explain the effects of OVs on band gaps and the ferromagnetism of SSO films, and the results suggested that an impurity energy level of OVs appeared near the Fermi level, causing the widening of the band gaps, which is consistent with our experimental results. Meanwhile, the room-temperature ferromagnetism was observed in the SSO films, and saturation magnetization increased gradually from 4.46 to 7.69 emu/cm 3 with decreasing the growth oxygen pressures.

This work was designed to separate and purify the saponin from Thelenota ananas with the highest anti-cholesterol ability using multiple chromatography and mass spectrometry analyses, and to systematically investigate the effect of the Thelenota ananas saponin on cholesterol metabolism in oxidized low-density lipoprotein (ox-LDL) induced macrophage foam cells. Desulfated holothurin A (desHA), which was finally identified as the targeted saponin with the highest activity in decreasing low-density lipoprotein-cholesterol (LDL-C), markedly inhibited the formation of foam cells derived from macrophages based on Oil Red O staining. In addition, desHA significantly blocked the synthesis of fatty acid synthetase while promoted intracellular cholesterol efflux. Furthermore, desHA inhibited the effects of ox-LDL on macrophage mRNA expression, which enhanced the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoAR) and suppressed the expression of SR-BI, ABCA1 and ABCG1, which further increased the levels of extracellular cholesterol and triglyceride. Blocking AKT and AMPK pathway and LXR synthesis revealed that desHA also regulated the contents of HMG-CoAR and eNOS via LXR/AKT/AMPK pathway. Thus, desHA played an essential role in cholesterol efflux and synthesis, which indicated desHA and Thelenota ananas are valuable resources to exploit new functional food and nutraceuticals.

Objective: The aim of this study was to examine shifts in the composition of the bacterial population in the intestinal tracts (ITs) of weaning piglets by antibiotic treatment using high-throughput sequencing., Methods: Sixty 28-d-old weaning piglets were randomly divided into two treatment groups. The Control group was treated with a basal diet without antibiotics. The Antibiotic group's basal diet contained colistin sulfate at a concentration of 20 g per ton and bacitracin zinc at a concentration of 40 g per ton. All of the pigs were fed for 28 days. Then, three pigs were killed, and the luminal contents of the jejunum, ileum, cecum, and colon were collected for DNA extraction and high-throughput sequencing., Results: The results showed that the average daily weight gain of the antibiotic group was significantly greater (p<0.05), and the incidence of diarrhea lower (p>0.05), than the control group. A total of 812,607 valid reads were generated. Thirty-eight operational taxonomic units (OTUs) that were found in all of the samples were defined as core OTUs. Twenty-one phyla were identified, and approximately 90% of the classifiable sequences belonged to the phylum Firmicutes. Forty-two classes were identified. Of the 232 genera identified, nine genera were identified as the core gut microbiome because they existed in all of the tracts. The proportion of the nine core bacteria varied at the different tract sites. A heat map was used to understand how the numbers of the abundant genera shifted between the two treatment groups., Conclusion: At different tract sites the relative abundance of gut microbiota was different. Antibiotics could cause shifts in the microorganism composition and affect the composition of gut microbiota in the different tracts of weaning piglets.