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452. Self-Serving Dishonesty Partially Substitutes Fairness in Motivating Cooperation When People Are Treated Fairly

Author

Li, D, Turel, O, Zhang, S, He, Q, Li, D, Turel, O, Zhang, S, and He, Q

Abstract

Fairness is a key expectation in social interactions. Its violation leads to adverse reactions, including non-cooperation and dishonesty. The present study aimed to examine how (1) fair (unfair) treatment may drive cooperation (defection) and honesty (self-serving dishonesty), (2) dishonesty primes further moral disengagement and reduced cooperation, and (3) dishonesty weakens (substitutes) the effect of fairness on cooperation. The prisoner's dilemma (Experiment 1 and 2) and die-rolling task (Experiment 2) were employed for capturing cooperation and dishonest behaviors, respectively. To manipulate perceived unfairness, participants were randomly assigned to play the prisoner's dilemma game, where players either choose more cooperation (fair condition) or defection (unfair condition). Results of Experiment 1 (n = 102) suggested that participants perceive higher unfairness and behave less cooperatively when the other player primarily chooses defection. Results of Exp. 2 (n = 240) (a) confirmed Exp. 1 results, (b) showed that players in the unfair condition also show more self-serving dishonest behavior, and (c) that dishonest behavior weakens the effect of fairness on cooperation. Together, these results extended previous work by highlighting the self-serving lies when the opponent is fair trigger higher cooperation, presumably as a means to alleviate self-reflective moral emotions or restore justice.

Published
2022

453. Heterogeneity within and among co-occurring foundation species increases biodiversity

Author

Thomsen, MS, Altieri, AH, Angelini, C, Bishop, MJ, Bulleri, F, Farhan, R, Fruhling, VMM, Gribben, PE, Harrison, SB, He, Q, Klinghardt, M, Langeneck, J, Lanham, BS, Mondardini, L, Mulders, Y, Oleksyn, S, Ramus, AP, Schiel, DR, Schneider, T, Siciliano, A, Silliman, BR, Smale, DA, South, PM, Wernberg, T, Zhang, S, Zotz, G, Thomsen, MS, Altieri, AH, Angelini, C, Bishop, MJ, Bulleri, F, Farhan, R, Fruhling, VMM, Gribben, PE, Harrison, SB, He, Q, Klinghardt, M, Langeneck, J, Lanham, BS, Mondardini, L, Mulders, Y, Oleksyn, S, Ramus, AP, Schiel, DR, Schneider, T, Siciliano, A, Silliman, BR, Smale, DA, South, PM, Wernberg, T, Zhang, S, and Zotz, G

Abstract

Habitat heterogeneity is considered a primary causal driver underpinning patterns of diversity, yet the universal role of heterogeneity in structuring biodiversity is unclear due to a lack of coordinated experiments testing its effects across geographic scales and habitat types. Furthermore, key species interactions that can enhance heterogeneity, such as facilitation cascades of foundation species, have been largely overlooked in general biodiversity models. Here, we performed 22 geographically distributed experiments in different ecosystems and biogeographical regions to assess the extent to which variation in biodiversity is explained by three axes of habitat heterogeneity: the amount of habitat, its morphological complexity, and capacity to provide ecological resources (e.g. food) within and between co-occurring foundation species. We show that positive and additive effects across the three axes of heterogeneity are common, providing a compelling mechanistic insight into the universal importance of habitat heterogeneity in promoting biodiversity via cascades of facilitative interactions. Because many aspects of habitat heterogeneity can be controlled through restoration and management interventions, our findings are directly relevant to biodiversity conservation.

Published
2022

454. Heterogeneity within and among co-occurring foundation species increases biodiversity (vol 13, 581, 2022)

Author

Thomsen, MS, Altieri, AH, Angelini, C, Bishop, MJ, Bulleri, F, Farhan, R, Fruhling, VMM, Gribben, PE, Harrison, SB, He, Q, Klinghardt, M, Langeneck, J, Lanham, BS, Mondardini, L, Mulders, Y, Oleksyn, S, Ramus, AP, Schiel, DR, Schneider, T, Siciliano, A, Silliman, BR, Smale, DA, South, PM, Wernberg, T, Zhang, S, Zotz, G, Thomsen, MS, Altieri, AH, Angelini, C, Bishop, MJ, Bulleri, F, Farhan, R, Fruhling, VMM, Gribben, PE, Harrison, SB, He, Q, Klinghardt, M, Langeneck, J, Lanham, BS, Mondardini, L, Mulders, Y, Oleksyn, S, Ramus, AP, Schiel, DR, Schneider, T, Siciliano, A, Silliman, BR, Smale, DA, South, PM, Wernberg, T, Zhang, S, and Zotz, G

Published
2022

455. Characteristics of toxicity occurrence patterns in concurrent chemoradiotherapy after induction chemotherapy for patients with locally advanced non-small-cell lung cancer: a pooled analysis based on individual patient data of CALGB/Alliance trials

Author

Yang, LZ, He, Q, Zhang, J, Ganti, AK, Stinchcombe, TE, Pang, H, Wang, X, Yang, LZ, He, Q, Zhang, J, Ganti, AK, Stinchcombe, TE, Pang, H, and Wang, X

Abstract

BACKGROUND: For patients with locally advanced non-small cell lung cancer (NSCLC), concurrent chemoradiotherapy is the foundational treatment strategy. Adding induction chemotherapy did not achieve a superior efficacy but increased the burden from toxicity. Accordingly, we retrospectively investigated the toxicity patterns through pooling individual patient data of the Cancer and Leukemia Group B (CALGB)/Alliance trials. METHODS: We included a total of 637 patients with unresectable stage III NSCLC who received induction chemotherapy with a platinum doublet and concurrent chemoradiotherapy and experienced at least one adverse event (AE) in CALGB 9130, 9431, 9534, 30105, 30106 and 39801 trials. The following toxicity occurrence patterns were evaluated: top 10 most frequent AEs, AE distribution by grade, rate of treatment discontinuation due to AEs, associations of AE occurrence with patient characteristics and treatment phase, the time to the first grade ≥3 AE occurrence and its associations with patient characteristics and treatment phase. RESULTS: The occurrence of AEs was the main reason accounting for treatment discontinuation (60 of 637 among all patients; 18 of 112 patients who experienced the induction phase only; 42 of 525 patients who experienced both phases). All patients experienced a total of 11,786 AEs (grade ≥3: 1,049 of 5,538 in induction phase, 1,382 of 6,248 in concurrent phase). Lymphocytes and white blood count were of top 3 grade ≥3 AEs that patients experienced the most in the either phase. Multivariable analysis found AE occurrence was associated with age ≥65 [any grade: odds ratio (OR) =1.44, 95% confidence interval (CI): 1.12-1.86] and the concurrent phase (grade ≥3: OR =1.86, 95% CI: 1.41-2.47; any grade: OR =1.47, 95% CI: 1.19-1.81). Patients in the concurrent phase were more likely and earlier to develop grade ≥3 AEs than those in the induction phase [hazard ratio (HR) =4.37, 95% CI: 2.52-7.59]. CONCLUSIONS: The report provides a better und

Published
2022

456. Novel Evaluation Method to Determine the Mixing Time in a Ladle Refining Process

Author

Zhou, X., Zhang, Y., He, Q., Ni, P., Yue, Q., Ersson, Mikael, Zhou, X., Zhang, Y., He, Q., Ni, P., Yue, Q., and Ersson, Mikael

Abstract

Mixing plays a key role in mass and heat transfer, as well as chemical reactions in various vessels involving agitation. Several studies have confirmed that the mixing time obtained from several monitor locations cannot reflect the mixing time for the whole bath because stirring situation in different locations is variable due to the change of operation schemes. It is proved that some zones with inefficient stirring cannot be monitored by applying a limited amount of probes in physical and mathematical models. This study provides a novel approach to quantify mixing time evaluation considering the tracer variation for the whole bath using a mathematical model. It was found that the mixing time obtained by considering the whole bath is more representative than that of the probe monitor method. Compared with the traditional probe method, about 50 to 70 pct longer mixing times were obtained for different operations by applying the volume track method. In addition, the volume integral of the concerned variable for the whole bath is more representative to determine the developed flow compared to the points monitoring method for a transient simulation., QC 20230613

Published
2022
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457. Role of abiotic drivers on crab burrow distribution in a saltmarsh wetland

Author

Chen, X., Zhou, Z., He, Q., Zhang, H., Bouma, T., Gong, Z., Townend, I., Zhang, C., Chen, X., Zhou, Z., He, Q., Zhang, H., Bouma, T., Gong, Z., Townend, I., and Zhang, C.

Abstract

Crab burrows play an important role in saltmarsh wetlands and are a useful indicator of wetland condition. The spatiotemporal distribution of crab burrows varies considerably in tidal wetlands. However, the reasons for these variations are poorly understood, in part, due to the limited availability of comprehensive field data. Based on a two-year continuous observation at a tidal wetland in the northern Jiangsu Coast, China, this study explored the relationship between crab burrow density and environmental variables, including median grain size, water content, organic matter content, soil salinity, and elevation. Our results show that the distribution of crab burrows was unimodal across the shore in winter and spring (Nov-Apr) when air temperature was relatively low, while bimodal in summer and autumn (May-Oct) when temperature was relatively high. The density of crab burrows was larger at areas with higher water content, higher organic matter content, and lower soil salinity, while it was lower with stronger hydrodynamics and lower suspended sediment concentration. Crab burrows were more abundant in vegetated areas than in un-vegetated areas. A backward stepwise model selection was performed based on R-square and Akaike information criterion (AIC) to distinguish the main driving factors that determine crab burrow distribution. Results suggested that the principal driving factors were organic matter content and soil salinity in all the seasons, with the addition of water content in warm seasons. Overall, this study provides a comprehensive field dataset for a more in-depth understanding of crab burrow distribution and a scientific basis for sustainable management of tidal wetlands.

Published
2022

458. A Supervised ML Applied Classification Model for Brain Tumors MRI.

Author

Yu, Z, He, Q, Yang, J, Luo, M, Yu, Z, He, Q, Yang, J, and Luo, M

Abstract

Brain Tumor originates from abnormal cells, which is developed uncontrollably. Magnetic resonance imaging (MRI) is developed to generate high-quality images and provide extensive medical research information. The machine learning algorithms can improve the diagnostic value of MRI to obtain automation and accurate classification of MRI. In this research, we propose a supervised machine learning applied training and testing model to classify and analyze the features of brain tumors MRI in the performance of accuracy, precision, sensitivity and F1 score. The result presents that more than 95% accuracy is obtained in this model. It can be used to classify features more accurate than other existing methods.

Published
2022

459. Developing machine learning models with multi-source environmental data to predict wheat yield in China

Author

Li, L, Wang, B, Feng, P, Li Liu, D, He, Q, Zhang, Y, Wang, Y, Li, S, Lu, X, Yue, C, Li, Y, He, J, Feng, H, Yang, G, Yu, Q, Li, L, Wang, B, Feng, P, Li Liu, D, He, Q, Zhang, Y, Wang, Y, Li, S, Lu, X, Yue, C, Li, Y, He, J, Feng, H, Yang, G, and Yu, Q

Abstract

Crop yield is controlled by different environmental factors. Multi-source data for site-specific soils, climates, and remotely sensed vegetation indices are essential for yield prediction. Algorithms of data-model fusion for crop growth monitoring and yield prediction are complicated and need to be optimized to deal with model uncertainty. This study integrated multi-source environmental variables (e.g., satellite-based vegetation indices, climate data, and soil properties) into random forest (RF) and support vector machine (SVM) models for wheat yield prediction in China. The performance of both RF and SVM models was investigated using different types of vegetation indices associated with other predictors. Relative importance and partial dependence analyses were used to identify the main predictors and their relationships with wheat yield. We found that using remotely sensed vegetation indices improved our model precision, and that near-infrared reflectance of terrestrial vegetation (NIRv) was slightly better than normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI) in predicting yield. NIRv was better in detecting climate stress on crops, and could capture more information regarding crop growth and yield formation. Compared with the SVM model, the RF model with NIRv and other covariates had better performance in wheat yield prediction, with R2 and RMSE being 0.74 and 758 kg/ha respectively. We also found that NIRv from jointing to heading was the most important predictor in determining yield, followed by solar radiation (especially during tillering–heading), relative humidity (during planting–tillering), soil organic carbon, and wind speed (throughout the growing season). In addition, wheat yield exhibited threshold-like responses to most factors based on our RF model. These threshold values can help to better understand how different environmental factors limit wheat yield, which will provide useful information for climate-adaptive cr

Published
2022

460. A highly selective superphane for ReO4− recognition and extraction

Author

Zhou, W, Li, A, Gale, PA, He, Q, Zhou, W, Li, A, Gale, PA, and He, Q

Abstract

Highly selective anion recognition and extraction is challenging and yet critical for removal of pollutants from the environment and the effective recovery of valuable chemicals from low-content (at sub-ppm or ppb level) sources. In this paper, we detail the gram-scale synthesis of a superphane 2, an anion receptor that selectively binds ReO4−. Superphane 2 can extract perrhenate from solid mixtures containing traces of ReO4− anion (as low as 200 ppb) and aqueous media with near 100% selectivity over large excesses of competing anions. Meanwhile, up to 99.99% of ReO4− can be separated from complex simulated aqueous waste streams containing ppm-level perrhenate via either liquid-liquid extraction or simple column adsorption. Importantly, after extraction or adsorption, superphane 2 can be recycled and reused by simple treatment with aqueous NaHCO3.

Published
2022

461. Identifying effective agricultural management practices for climate change adaptation and mitigation: A win-win strategy in South-Eastern Australia

Author

He, Q, Liu, DL, Wang, B, Li, L, Cowie, A, Simmons, A, Zhou, H, Tian, Q, Li, S, Li, Y, Liu, K, Yan, H, Harrison, MT, Feng, P, Waters, C, Li, GD, de Voil, P, Yu, Q, He, Q, Liu, DL, Wang, B, Li, L, Cowie, A, Simmons, A, Zhou, H, Tian, Q, Li, S, Li, Y, Liu, K, Yan, H, Harrison, MT, Feng, P, Waters, C, Li, GD, de Voil, P, and Yu, Q

Abstract

CONTEXT: Farming systems face dual pressures of reducing greenhouse gas (GHG) emissions to mitigate climate change and safeguarding food security to adapt to climate change. Building soil organic carbon (SOC) is proposed as a key strategy for climate change mitigation and adaptation. However, practices that increase SOC may also increase nitrous oxide (N2O) emissions, and impact crop yields and on-farm income. A comprehensive assessment of the effects of different management practices on trade-offs between GHG emissions and agricultural systems profitability under climate change is needed. OBJECTIVE: We aimed to: (1) analyze the long-term trends of SOC and N2O emissions, and ascertain whether the croplands of the study region are net GHG sources or sinks under climate change; (2) quantify the GHG abatement on a gross margin basis; (3) identify effective management practices that could achieve a win-win strategy; and (4) investigate sources of uncertainty in estimates of GHG emissions and gross margins under climate change. METHODS: APSIM was used to simulate the effects of three crop residue retention rates (10%, 50% and 100%), and six representative crop rotations (wheat-canola, wheat-field pea-wheat-canola, wheat-field pea-wheat-oats, wheat-wheat-barley, wheat-wheat-canola, and wheat-wheat-oats) under two Shared Socio-economic Pathways scenarios (SSP245 and SSP585) using climate projections from 27 GCMs. GHG emissions and gross margins from 1961 to 2092 were assessed across 204 study sites in southeastern Australia. RESULTS AND CONCLUSIONS: Our results showed that residue retention can turn the soil from a carbon source (10% retention, 304–450 kg CO2-eq ha−1 yr−1) to a carbon sink (100% retention, −269 ∼ −57 kg CO2-eq ha−1 yr−1), and the potential of carbon sequestration was partly offset by concomitantly increased N2O emissions. The wheat-wheat-canola rotation with full residue retention was shown to be a win-win solution with both large potential of GHG abatemen

Published
2022

462. Testing of a model for risk factors for eating disorders and higher weight among emerging adults: Baseline evaluation

Author

Fuller-Tyszkiewicz, M., Rodgers, R. F., Maiano, C., Mellor, D., Sicilia, A., Markey, C. H., Aime, A., Dion, J., Pietrabissa, Giada, Lo Coco, G., Caltabiano, M. L., Strodl, E., Alcaraz-Ibanez, M., Begin, C., Blackburn, M. -E., Castelnuovo, Gianluca, Granero-Gallegos, A., Gullo, S., Hayami-Chisuwa, N., He, Q., Manzonie, G. M., Probst, M., Mccabe, M., Pietrabissa G. (ORCID:0000-0002-5911-5748), Castelnuovo G. (ORCID:0000-0003-2633-9822), Fuller-Tyszkiewicz, M., Rodgers, R. F., Maiano, C., Mellor, D., Sicilia, A., Markey, C. H., Aime, A., Dion, J., Pietrabissa, Giada, Lo Coco, G., Caltabiano, M. L., Strodl, E., Alcaraz-Ibanez, M., Begin, C., Blackburn, M. -E., Castelnuovo, Gianluca, Granero-Gallegos, A., Gullo, S., Hayami-Chisuwa, N., He, Q., Manzonie, G. M., Probst, M., Mccabe, M., Pietrabissa G. (ORCID:0000-0002-5911-5748), and Castelnuovo G. (ORCID:0000-0003-2633-9822)

Abstract

Although a range of risk factors have been identified for disordered eating and weight status, the breadth of risk factors have been rarely considered within a single, comprehensive model. The robustness of these findings across countries also remains an open question. The present study sampled 6272 participants aged 18–30 years from eight countries in an attempt to evaluate combined and unique predictors for these two conditions, and to explore possible cross-country differences in these models. Participants completed a range of demographic, biological, behavioral, psychological, and sociocultural measures to test a comprehensive model of the contributions of these predictors for disordered eating and weight-related constructs (binge eating, body mass index, compensatory behaviors, dietary restraint, drive for muscularity, and drive for leanness). Structural invariance testing within a multigroup path analysis framework revealed that a single model across the eight countries provided poor model fit. Freeing of 22% of parameters across countries provided excellent fit and a satisfactory compromise for country-invariant and country-variant parameters in the model. Overall, predictors accounted for between 15% and 60% of variance in the outcome measures, with lowest explained variance for the disordered eating outcomes. Significant unique contributions to prediction were observed for each of the five risk factor variable types and across the eight countries. Thus, the findings show strong support for this model as an explanatory framework of both disordered eating and weight status.

Published
2022

466. A Computer-Assisted Test Design and Diagnosis System for Use by Classroom Teachers

Author

He, Q. and Tymms, P.

Abstract

Computer-assisted assessment (CAA) has become increasingly important in education in recent years. A variety of computer software systems have been developed to help assess the performance of students at various levels. However, such systems are primarily designed to provide objective assessment of students and analysis of test items, and focus has been mainly placed on higher and further education. Although there are commercial professional systems available for use by primary and secondary educational institutions, such systems are generally expensive and require skilled expertise to operate. In view of the rapid progress made in the use of computer-based assessment for primary and secondary students by education authorities here in the UK and elsewhere, there is a need to develop systems which are economic and easy to use and can provide the necessary information that can help teachers improve students' performance. This paper presents the development of a software system that provides a range of functions including generating items and building item banks, designing tests, conducting tests on computers and analysing test results. Specifically, the system can generate information on the performance of students and test items that can be easily used to identify curriculum areas where students are under performing. A case study based on data collected from five secondary schools in Hong Kong involved in the Curriculum, Evaluation and Management Centre's Middle Years Information System Project, Durham University, UK, has been undertaken to demonstrate the use of the system for diagnostic and performance analysis.

Published
2005
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467. Effect of midodrine on the prognosis of patients with septic shock: a systematic review and meta-analysis.

Author

HE, Q.-F., XING, X.-K., WANG, T.-Q., JIANG, Z.-X., and ZHANG, G.

Abstract

OBJECTIVE: This study aims to assess the efficacy and safety of midodrine on treating patients with septic shock. MATERIALS AND METHODS: Literature search was conducted in PubMed, the Cochrane Library, and Embase. The Mantel-Haenszel method was used to calculate pooled relative risks (RRs) and 95% confidence intervals (95% CI). The mean differences (MD) or standardized mean difference (SMD) were calculated using the inverse variance for continuous variables. Data analysis was performed using Review Manager 5.3. RESULTS: A total of 6 studies were finally included in this meta-analysis. Adding midodrine to patients with septic shock was associated with a reduction in hospital mortality [risk ratio (RR) 0.76; 95% CI, 0.57-1.00; p=0.05] and intensive care unit (ICU) mortality (RR 0.59; 95% CI, 0.41-0.87; p=0.008). However, there were no significant differences in the duration of intravenous vasopressors [standardized mean difference (SMD) -0.18; 95% CI, -0.47-0.11; p=0.23], intravenous vasopressor reinstitution (RR 0.58; 95% CI, 0.19-1.80; p=0.35), the length of ICU stay [mean difference (MD) -0.53 days; 95% CI, -2.24- 1.17; p=0.54], and the length of hospital stay (MD -2.40 days; 95% CI, -5.26-0.46; p=0.10) between midodrine group and intravenous vasopressor alone group. CONCLUSIONS: The additional use of midodrine might reduce hospital mortality and ICU mortality in patients with septic shock. More high-quality randomized controlled trials are needed to verify this conclusion. [ABSTRACT FROM AUTHOR]

Published
2023

468. Microbiome-scale analysis of aerosol facemask contamination during nebulization therapy in hospital.

Author

Swanson, C.S., Dhand, R., Cao, L., Ferris, J., Elder, C.S., and He, Q.

Abstract

Microbial contamination of aerosol facemasks could be a source of nosocomial infections during nebulization therapy in hospital, prompting efforts to identify these contaminants. Identification of micro-organisms in medical devices has traditionally relied on culture-dependent methods, which are incapable of detecting the majority of these microbial contaminants. This challenge could be overcome with culture-independent sequencing-based techniques that are suited for the profiling of complex microbiomes. To characterize the microbial contaminants in aerosol facemasks used for nebulization therapy, and identify factors influencing the composition of these microbial contaminants with the acquisition and analysis of comprehensive microbiome-scale profiles using culture-independent high-throughput sequencing. Used aerosol facemasks collected from hospitalized patients were analysed with culture-independent 16S rRNA gene-based amplicon sequencing to acquire microbiome-scale comprehensive profiles of the microbial contaminants. Microbiome-based analysis was performed to identify potential sources of microbial contamination in facemasks. Culture-independent high-throughput sequencing was demonstrated for the capacity to acquire microbiome-scale profiles of microbial contaminants on aerosol facemasks. Microbial source identification enabled by the microbiome-scale profiles linked microbial contamination on aerosol facemasks to the human skin and oral microbiota. Antibiotic treatment with levofloxacin was found to reduce contamination of the facemasks by oral microbiota. Sequencing-based microbiome-scale analysis is capable of providing comprehensive characterization of microbial contamination in aerosol facemasks. Insight gained from microbiome-scale analysis facilitates the development of effective strategies for the prevention and mitigation of the risk of nosocomial infections arising from exposure to microbial contamination of aerosol facemasks, such as targeted elimination of potential sources of contamination. [ABSTRACT FROM AUTHOR]

Published
2023
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469. Potential biomarkers for the prognosis and treatment of HCC immunotherapy.

Author

LI, H., HE, Q., ZHOU, G.-M., WANG, W.-J., SHI, P.-P., and WANG, Z.-H.

Abstract

OBJECTIVE: The liver is a unique organ containing large populations of immune cells. Immunotherapy for liver cancer is a promising yet particularly challenging method. Therefore, it harbors great significance for the identification of immune-related subtypes and the potential therapeutic targets for hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Firstly, we classified the HCC samples downloaded from the dataset of Cancer Genome Atlas (TCGA) into two clusters based on the immune cell infiltration. Thereafter, we identified the significant module and regulatory factors using the weighted gene co-expression network analysis (WGCNA). The immune competence of the regulatory factors was delineated through the ESTIMATE algorithm, the analysis of the tumor microenvironment, and pan-cancer analysis. In the single-cell RNA sequencing analysis, we further explored the immune competence of regulatory factors. We also collected the potential drugs targeting the regulatory factors. In addition, we constructed lncRNA-miRNA-mRNA interaction regulatory networks. Finally, western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were conducted to verify the protein expression of regulatory genes in HCC cell lines and tissues. RESULTS: According to the immune cell infiltration, two immune-related subtypes-cluster 1 and cluster 2-were found. Patients in cluster 2 had a more significant immune infiltration than in cluster 1. Afterward, six significant regulatory genes were identified through WGCNA, and the expression in cluster 2 was high in cluster 1. We performed a comprehensive analysis to clarify the immune signature. The results showed that the six genes had significant immunological competence. Moreover, the expression of the six genes was similar to the subtypes' classification. In the analysis of the prognosis value, patients in cluster 2 had a better prognosis. In addition, the lncRNA in the lncRNA-miRNA-mRNA interaction regulatory networks was located in the nucleus and cytoplasm. In the single-cell RNA sequencing analysis, the six genes were related to the immune cell. We also identified potential drugs for CD6 and CLEC12A, which may provide potential therapeutic drugs. Finally, the regulatory genes were verified in the western blot and quantitative real-time polymerase chain reaction. CONCLUSIONS: The classification into two clusters based on the immune cell infiltration may provide a promising prospect for HCC through immunotherapy. The six regulatory genes may be potential therapeutic targets in the treatment of HCC. [ABSTRACT FROM AUTHOR]

Published
2023

477. Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998–2012

Author

van Gent, M., Heuvelman, C. J., van der Heide, H. G., Hallander, H. O., Advani, A., Guiso, N., Wirsing von Kőnig, C. H., Vestrheim, D. F., Dalby, T., Fry, N. K., Pierard, D., Detemmerman, L., Zavadilova, J., Fabianova, K., Logan, C., Habington, A., Byrne, M., Lutyńska, A., Mosiej, E., Pelaz, C., Gröndahl-Yli-Hannuksela, K., Barkoff, A. M., Mertsola, J., Economopoulou, A., He, Q., and Mooi, F. R.

Published
2015
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482. Deterministic switching of ferromagnetism at room temperature using an electric field

Author

Heron, J. T., Bosse, J. L., He, Q., Gao, Y., Trassin, M., Ye, L., Clarkson, J. D., Wang, C., Liu, Jian, Salahuddin, S., Ralph, D. C., Schlom, D. G., Iniguez, J., Huey, B. D., and Ramesh, R.

Subjects
Ferromagnetism -- Research, Electric fields -- Usage, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): J. T. Heron (corresponding author) [1]; J. L. Bosse [2]; Q. He [3]; Y. Gao [4, 5]; M. Trassin [6]; L. Ye [2]; J. D. Clarkson [7]; C. Wang [...]

Published
2014
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487. Crystal structure of SPD_0310

Author

Cao, K., primary, Zhang, T., additional, Li, N., additional, Yang, X., additional, Ding, J., additional, He, Q., additional, and Sun, X., additional

Published
2022
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488. Coherent photoproduction of the neutral-pion and η -meson on the deuteron at incident energies below 1.15 GeV

Author

Ishikawa, T., primary, Fix, A., additional, Fujimura, H., additional, Fukasawa, H., additional, Hashimoto, R., additional, He, Q., additional, Honda, Y., additional, Iwata, T., additional, Kaida, S., additional, Kasagi, J., additional, Kawano, A., additional, Kuwasaki, S., additional, Maeda, K., additional, Masumoto, S., additional, Miyabe, M., additional, Miyahara, F., additional, Mochizuki, K., additional, Muramatsu, N., additional, Nakamura, A., additional, Nawa, K., additional, Obara, Y., additional, Ogushi, S., additional, Okada, Y., additional, Okamura, K., additional, Onodera, Y., additional, Ozawa, K., additional, Sakamoto, Y., additional, Sato, M., additional, Shimizu, H., additional, Sugai, H., additional, Suzuki, K., additional, Tajima, Y., additional, Takahashi, S., additional, Taniguchi, Y., additional, Tsuchikawa, Y., additional, Yamazaki, H., additional, Yamazaki, R., additional, and Yoshida, H. Y., additional

Published
2022
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493. MicroRNA-21 Mediates a Positive Feedback on Angiotensin II-Induced Myofibroblast Transformation

Author

Li D, Mao C, Zhou E, You J, Gao E, Han Z, Fan Y, He Q, and Wang C

Subjects
myocardial infarction, erk/tgf-β/smad signaling pathway, cardiac fibrosis, sprouty1, Pathology, RB1-214, Therapeutics. Pharmacology, RM1-950, microrna-21
Abstract

Dongjiu Li,1,* Chengyu Mao,1,* En Zhou,1,* Jiayin You,2,* Erhe Gao,3 Zhihua Han,1 Yuqi Fan,1 Qing He,1 Changqian Wang1 1Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of China; 2Department of Emergency, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of China; 3Center for Translational Medicine, Department of Pharmacology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA*These authors contributed equally to this workCorrespondence: Changqian Wang Email wangcqdr17@163.comObjective: Post myocardial infarction (MI) fibrosis has been identified as an important factor in the progression of heart failure. Previous studies have revealed that microRNA-21 (miR-21) plays an important role in the pathogenesis of fibrosis. The purpose of this study was to explore the role of miR-21 in post-MI cardiac fibrosis.Material and Methods: MI was established in wild-type (WT) and miR-21 knockout (KO) mice. Primary mice cardiac fibroblasts (CFs) were isolated from WT and miR-21 KO mice and were treated with angiotensin II (Ang II) or Sprouty1 (Spry1) siRNA. Histological analysis and echocardiography were used to determine the extent of fibrosis and cardiac function.Results: Compared with WT mice, miR-21 KO mice displayed smaller fibrotic areas and decreased expression of fibrotic markers and inflammatory cytokines. In parallel, Ang II-induced myofibroblasts transformation was partially inhibited upon miR-21 KO in primary CFs. Mechanistically, we found that the expression of Spry1, a previously reported target of miR-21, was markedly increased in miR-21 KO mice post MI, further inhibiting ERK1/2 activation. In vitro studies showed that Ang II activated ERK1/2/TGF-β/Smad2/3 pathway. Phosphorylated Smad2/3 further enhanced the expression of α-SMA and FAP and may promote the maturation of miR-21, thereby downregulating Spry1. Additionally, these effects of miR-21 KO on fibrosis were reversed by siRNA-mediated knockdown of Spry1.Conclusion: Our findings suggest that miR-21 promotes post-MI fibrosis by targeting Spry1. Furthermore, it mediates a positive feedback on Ang II, thereby inducing the ERK/TGF-β/Smad pathway. Therefore, targeting the miR-21–Spry1 axis may be a promising therapeutic option for ameliorating post-MI cardiac fibrosis.Keywords: cardiac fibrosis, myocardial infarction, microRNA-21, Sprouty1, ERK/TGF-β/Smad signaling pathway

Published
2020

494. miR145 Regulates the Proliferation and Apoptosis of Rat Vascular Endothelial Cells under Hyperglycemia by Targeting the ANGPT2 Gene and Involving the NFκB Signaling Pathway

Author

Zhang W, Tang XH, Zhang JJ, and He Q

Subjects
hyperglycemia, mir-145, RC581-951, diabetic vasculopathy, Specialties of internal medicine, rat aortic endothelial cells (raecs), angiopoietin-2 (angpt2)
Abstract

Wen Zhang,1 Xin-Hua Tang,2 Jin-Juan Zhang,3 Quan He4 1Clinical Medical Research Center and Yunnan Provincial Key Laboratory of Clinical Virology (2018DG010), First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, People’s Republic of China; 2Center of Genetic Diagnosis, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, People’s Republic of China; 3Kunming Institute of Zoology, Chinese Academy of Science (CAS), Kunming, Yunnan, 650223, People’s Republic of China; 4Emergency Department, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, 650032, People’s Republic of ChinaCorrespondence: Quan HeEmergency Department, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Road, Xishan District, Kunming 650032, Yunnan Province, People’s Republic of ChinaTel +86 871 6363 9921Fax +86 871 6362 7731Email hequan2009@yeah.netPurpose: A majority of diabetes mellitus patients with disturbances of glucose metabolism present with vascular complications. This study aimed to explore regulatory mechanisms of miR145 and its potential target gene ANGPT2 on diabetic vasculopathy under hyperglycemia.Methods: Based on the fact that miR145 is detected in rat aortic endothelial cells (RAECs) under hyperglycemia, RAECs were transfected with miR145 mimics/inhibitor for further confirmation. RAEC proliferation was detected with CCK8 assays, and cell apoptosis and CD34+-cell population with annexinV–PI staining and anti-CD34FITC on flow cytometry, respectively. Then, qPCR and Western blot were applied to detect mRNA and protein expression of ANGPT2 and involved pathway factor NFκB p65. Subsequently, dual luciferase–reporter gene analysis was utilized to verify whether miR145 acted directly upon the 3ʹUTR of ANGPT2 mRNA.Results: The ANGPT2 gene was confirmed to be a direct target of miR145. miR145 mimics markedly downregulated the expression of ANGPT2 and NFκB p65, boosted the percentage of the CD34+ phenotype, and promoted proliferation and suppressed apoptosis of RAECs under hyperglycemia.Conclusion: miR145 might regulate the viability of RAECs via targeting ANGPT2 and involving NFκB signaling to exert a protective effect on diabetic vasculature.Keywords: diabetic vasculopathy, miR145, angiopoietin 2, ANGPT2, rat aortic endothelial cells, RAECs, hyperglycemia

Published
2020

495. A Variant of Leptin Gene Decreases the Risk of Gastric Cancer in Chinese Individuals: Evidence from a Case–Control Study

Author

Ma R and He Q

Subjects
lcsh:Therapeutics. Pharmacology, gastric cancer, case-control study, education, lcsh:RM1-950, g19a polymorphism, lep, hormones, hormone substitutes, and hormone antagonists
Abstract

Renjie Ma, Qi He Department of Infectious Disease, The People’s Hospital of Danyang, Affiliated Danyang Hospital of Nantong University, Danyang, Jiangsu Province, 212300, People’s Republic of ChinaCorrespondence: Qi He Tel/fax +86-511-86553015Email heqi3214@163.comBackground: A host of studies have explored the potential connection between leptin (LEP) G19A polymorphism and the risk of cancers, but the relationship between gastric cancer (GC) susceptibility and LEP G19A polymorphism was not revealed before. The aim of this study was to investigate this relationship in Chinese Han population.Methods: Thus, this case–control study with 380 GC cases and 465 controls was designed to unearth the link between LEP G19A polymorphism and GC susceptibility. Genotyping was accomplished by a custom-made 48-Plex SNP scanTM kit. Relative LEP gene expression was detected by real-time reverse transcription-polymerase chain reaction.Results: LEP G19A polymorphism was shown to relate with a decreased risk of GC. Subgroup analyses uncovered significant connections in the males, nondrinkers, and those at age < 60 years. G19A polymorphism was also linked with tumor size and location and pathological type of GC. Last, LEP gene expression in gastric tissues was considerably less than in control tissues.Conclusion: This study shows that G19A polymorphism of LEP gene is linked with a lower risk of GC in the tested Chinese Han individuals.Keywords: LEP, gastric cancer, G19A polymorphism, case-control study

Published
2020

496. Correlation Between Ideal Cardiovascular Health Metrics and Plasma hs-CRP Levels in a North China Population: One Four-Year Follow-Up Study

Author

Chen J, Xu L, He Q, Wu S, Hu D, and Lu K

Subjects
lcsh:R5-920, ideal cardiovascular health metrics, highly sensitive c-reactive protein；cardiovascular diseases, lcsh:Medicine (General)
Abstract

Jia Chen,1 Liuyue Xu,2 Quan He,3 Shouling Wu,4 Dayi Hu,5 Kai Lu3 1Department of Clinical Nutrition, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of China; 2Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of China; 3Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of China; 4Department of Cardiology, Kailuan General Hospital, Hebei United University, Tangshan 100816, People’s Republic of China; 5Heart Center, Peking University People’s Hospital, Beijing 100044, People’s Republic of ChinaCorrespondence: Kai LuDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, No. 1, Yixueyuan Road, Chongqing 400016, People’s Republic of ChinaTel/Fax +86 23 89011562Email lukai2013@foxmail.comBackground: This prospective cohort study aimed to evaluate the potential association of ideal cardiovascular health metrics with the plasma levels of highly sensitive C-reactive protein (hs-CRP) among people in North China.Methods: A total of 80,968 participants were included in this study at baseline. Data relating to seven health metrics and plasma hs-CRP levels were collected at baseline and the end of follow-up. The change in health metrics of each individual was compared and recorded. The potential association of gain or loss of each health metric, as well as the change in the total number of health metrics that each individual had during follow-up, with the risk of increased hs-CRP level, were explored by multiple Cox proportional hazards models.Results: A total of 72,321 participants (94.51%) completed the follow-up, and the average follow-up time was 4.1 years. Ideal body mass index (BMI), physical activity, smoking status and total cholesterol all had significant impacts on hs-CRP levels, with ideal BMI having the largest impact. Losing ideal BMI during follow-up raised the probability of hs-CRP increase during the follow-up time by 1.72 (95% CI, 1.59– 1.86) times for men and 2.05 (95% CI, 1.76– 2.39) times for women. The effects of ideal salt intake and blood pressure on hs-CRP levels were uncertain. Changes in the total number of ideal metrics for each individual had a significant cumulative effect on hs-CRP. The hazard ratio of loss of four to six health metrics during follow-up on the risk of hs-CRP increase was 1.69 (95% CI, 1.38– 2.06) for men and 1.52 (95% CI, 1.13– 2.04) for women.Conclusion: Loss or gain of ideal cardiovascular health metrics is associated with significant fluctuations in plasma hs-CRP levels. The cardiovascular benefits brought by the ideal health metrics are mediated at least in part by reducing systemic inflammation in the body.Keywords: ideal cardiovascular health metrics, highly sensitive C-reactive protein, cardiovascular diseases

Published
2020

497. Research Status and Outlook of PD-1/PD-L1 Inhibitors for Cancer Therapy

Author

Ai L, Chen J, Yan H, He Q, Luo P, Xu Z, and Yang X

Subjects
immune checkpoint inhibitors, clinical trials, lcsh:Therapeutics. Pharmacology, pd-l1, monoclonal antibody, lcsh:RM1-950, pd-1, adverse events
Abstract

Leilei Ai, Jian Chen, Hao Yan, Qiaojun He, Peihua Luo, Zhifei Xu, Xiaochun Yang Center for Drug Safety Evaluation and Research of Zhejiang University, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of ChinaCorrespondence: Zhifei Xu; Xiaochun Yang Tel +86-571-88206915Fax +86-571-88208400Email xzfzjut@zju.edu.cnAbstract: PD-1/PD-L1 inhibitors are a group of immune checkpoint inhibitors as front-line treatment of multiple types of cancer. However, the serious immune-related adverse reactions limited the clinical application of PD-1/PD-L1 monoclonal antibodies, despite the promising curative effects. Therefore, it is urgent to develop novel inhibitors, such as small molecules, peptides or macrocycles, targeting the PD-1/PD-L1 axis to meet the increasing clinical demands. Our review discussed the mechanism of action of PD-1/PD-L1 inhibitors and presented clinical trials of currently approved PD-1/PD-L1 targeted drugs and the incidence of related adverse reactions, helping clinicians pay more attention to them, better formulate their intervention and resolution strategies. At last, some new inhibitors whose patent have been published are listed, which provide development ideas and judgment basis for the efficacy and safety of novel PD-1/PD-L1 inhibitors.Keywords: PD-1, PD-L1, immune checkpoint inhibitors, clinical trials, adverse events, monoclonal antibody

Published
2020

498. miR-100 rs1834306 A>G Increases the Risk of Hirschsprung Disease in Southern Chinese Children

Author

Zhu Y, Lin A, Zheng Y, Xie X, He Q, and Zhong W

Subjects
mir-100, lcsh:Therapeutics. Pharmacology, hirschsprung disease, lcsh:RM1-950, susceptibility, polymorphism
Abstract

Yun Zhu,* Ao Lin,* Yi Zheng, Xiaoli Xie, Qiuming He, Wei Zhong Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, Guangdong 510623, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei ZhongDepartment of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, Guangdong 510623, People’s Republic of ChinaTel/ Fax +86-18902268667Email zhongwei@gwcmc.orgBackground: Hirschsprung disease (HSCR) is a rare congenital gastrointestinal disease characterized by the absence of intestinal submucosal and myometrial ganglion cells. Recently, researches indicated that miR-100 regulated the growth, differentiation and apoptosis of neurons, and affected the functions of HSCR-associated pathways. While miR-100 rs1834306 A>G polymorphism was shown to modify the susceptibility to tumors, the association between this polymorphism and HSCR susceptibility is still unknown.Methods: This was a case–control study consisting of 1470 HSCR cases and 1473 controls from southern China. DNA was genotyped by TaqMan real-time PCR. Odds ratios (ORs) and 95% confidence intervals (CIs) were used as statistical indicators.Results: We found that miR-100 rs1834306 G allele and GG genotype significantly increased HSCR susceptibility (GG vs AA: adjusted OR=1.31, 95% CI=1.04– 1.64, P=0.020; G vs A: adjusted OR=1.12, 95% CI=1.01– 1.25, P=0.041; GG vs AA/AG: adjusted OR=1.30, 95% CI=1.07– 1.59, P=0.010). In the stratified analysis, miR-100 rs1834306 GG genotype carriers had higher risk to develop HSCR in all clinical subtypes when compared with those with AA/AG genotypes, and OR was rising with HSCR aggravation (SHSCR: adjusted OR=1.28, 95% CI=1.03– 1.59, P=0.029; LHSCR: adjusted OR=1.48, 95% CI=1.06– 2.07, P=0.020; TCA: adjusted OR=2.12, 95% CI=1.22– 3.69, P=0.008).Conclusion: Our findings suggested that miR-100 rs1834306 A>G polymorphism was associated with increased HSCR susceptibility in southern Chinese children. Furthermore, miR-100 rs1834306 GG genotype had a greater genetic pathopoiesis in severe HSCR.Keywords: Hirschsprung disease, miR-100, polymorphism, susceptibility

Published
2020

499. POLE Mutation Characteristics in a Chinese Cohort with Endometrial Carcinoma

Author

Li Y, He Q, Li S, Wen X, Ye L, Wang K, and Wan X

Subjects
chinese cohort, polymerase ɛ (pole) mutation, endometrial carcinoma, curettage specimens, hysterectomy, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282
Abstract

Yiran Li1 *, Qizhi He2 *, Shuangdi Li,1 Xiaoli Wen,3 Lei Ye,1 Kai Wang,3 Xiaoping Wan1 1Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China; 2Department of Pathology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China; 3Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Kai Wang Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Shanghai 200040, People’s Republic of ChinaTel +86 (021)-5403-7438Email kaiwangcn@yahoo.comXiaoping Wan Department of Gynecology, Shanghai First Maternity and Infant Hospital, Shanghai 200040, People’s Republic of ChinaTel +86 136-5191-7177Email wanxiaoping62@126.comObjective: To study the characteristics of polymerase epsilon (POLE) exonuclease domain mutations in Chinese patients with endometrial carcinoma (EC).Methods: This study analyzed data from 529 patients with EC in The Cancer Genome Atlas (TCGA) and 467 EC patients evaluated at the Shanghai First Maternity and Infant Hospital (SFMIH). POLE mutation heterogeneity was analyzed in paired curettage and hysterectomy samples from 120 SFMIH patients. Sanger sequencing identified mutations in the POLE exonuclease domain, and correlations between POLE mutation status and various clinicopathological features were determined by chi-squared testing and Cohen’s kappa analysis, with Kaplan–Meier survival curves generated to assess correlations between POLE mutation status and overall survival (OS).Results: Thirty-five mutations were identified in 467 samples (7.5%), and novel mutations were detected in the SFMIH cohort. Compared to the TCGA cohort, the SFMIH cohort had fewer POLE mutations when matched by age (< 60) and histology (endometrioid) (p < 0.001 and p = 0.010, respectively). In our study cohort, POLE mutations were significantly associated with adjuvant treatment (p = 0.029), and patients with POLE mutations who underwent chemoradiotherapy had a poor OS (p < 0.0001). Notably, shorter OS was significantly associated with POLE mutations in hysterectomy samples from patients aged > 60 years or with stage I disease in the paired curettage-hysterectomy group.Conclusion: The significant difference in POLE mutation profiles between the TCGA and SFMIH cohorts, as well as the poor consistency between the curettage and hysterectomy samples, suggests that different parameters need to be applied to determine the prognosis of patients with EC in China.Keywords: endometrial carcinoma, polymerase epsilon mutation, POLE mutation, Chinese cohort, hysterectomy, curettage specimens

Published
2020

500. Triptolide Inhibits the Proliferation of HaCaT Cells Induced by IL22 via Upregulating miR-181b-5p

Author

He Q, Zhang B, Hu F, Long J, Shi Q, Pi X, Chen H, and Li J

Subjects
keratinocytes, mir-181b, lcsh:Therapeutics. Pharmacology, triptolide, lcsh:RM1-950, psoriasis, differentiation
Abstract

Qi He,1,2,* Bo Zhang,1,2,* Feng Hu,3 Jianwen Long,1,2 Quan Shi,1,2 Xianming Pi,1,2 Hongxiang Chen,4 Jiawen Li4 1Department of Dermatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430061, People’s Republic of China; 2Department of Dermatology, Hubei Province Academy of Traditional Chinese Medicine, Wuhan, Hubei 430074, People’s Republic of China; 3Department of Dermatology, Wuhan No.1 Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, People’s Republic of China; 4Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Quan ShiDepartment of Dermatology, Hubei Provincial Hospital of Traditional Chinese Medicine, No. 4 Huanyuanshan, Wuchang District, Wuhan, Hubei 430061, People’s Republic of ChinaEmail shi_quan999@126.comBackground: Evidence has been shown that triptolide was effective in the treatment of psoriasis; however, the mechanisms remain poorly understood. Thus, this study aimed to investigate the role of triptolide on the proliferation and differentiation of HaCaT cells which are treated with IL22 to mimic abnormal proliferation/differentiation in keratinocyte of psoriasis.Materials and Methods: HaCaT cells were transfected with miR-181b-5p antagomir for 24 h, and then exposed to 10 μM Triptolide for 24 h, following by 100 ng/mL of IL22 for 24 h. In addition, the proliferation and cell cycle distribution in HaCaT cells were assessed by immunofluorescence or flow cytometry assays, respectively.Results: Triptolide obviously upregulated the level of miR-181b-5p in HaCaT cells. In addition, triptolide significantly inhibited IL22-induced proliferation of HaCaT cells via inducing cell cycle arrest. Moreover, IL22 markedly inhibited the differentiation of HaCaT cells, and this phenomenon was reversed by triptolide treatment. In contrast, the effects of triptolide on the proliferation and differentiation in IL22-stimulated HaCaT cells were notably reversed by miR-181b-5p antagomir. Moreover, dual-luciferase assay showed that E2F5 was the direct target of miR-181b-5p in HaCaT cells. Meanwhile, upregulation of miR-181b-5p obviously decreased the level of E2F5 in HaCaT cells.Conclusion: In this study, we found that triptolide could inhibit the proliferation and promote the differentiation in IL22-stimulated keratinocytes via upregulating miR-181b-5p. These data indicated that triptolide may be a potential agent for the treatment of psoriasis.Keywords: psoriasis, triptolide, keratinocytes, miR-181b, differentiation

Published
2020

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