Search

Electron micrographs of a variably titled specimen can be blended by computer to form an image of any selected specimen plane. Thus, one can examine a tissue section layer by layer with an effective depth of focus less than 30 A. Ribosomes so observed in rat liver have some resemblances to isolated, tungsten-shadowed particles.

With use of an electronic picture-scanning device and a digital computer, electron micrographs taken of a specimen along several different directions can be superimposed to form a montage that is more informative than the component images. Preliminary results indicate that one may thus study unstained, unshadowed biological material at high resolution.