639 results on '"Fumihiko Sato"'
Search Results
452. Electrophysiological and morphological properties of rat supratrigeminal premotor neurons targeting the trigeminal motor nucleus.
- Author
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Shiro Nakamura, Kiyomi Nakayama, Ayako Mochizuki, Fumihiko Sato, Tahsinul Haque, Atsushi Yoshida, and Tomio Inoue
- Subjects
ELECTROPHYSIOLOGY ,PREMOTOR cortex ,TRIGEMINAL nerve ,CELL morphology ,CALCIUM channels ,LABORATORY rats - Abstract
The electrophysiological and morphological characteristics of premotor neurons in the supratrigeminal region (SupV) targeting the trigeminal motor nucleus (MoV) were examined in neonatal rat brain stem slice preparations with Ca
2+ imaging, whole cell recordings, and intracellular biocytin labeling. First, we screened SupV neurons that showed a rapid rise in intracellular free Ca2+ concentration ([Ca2+ ]i ) after single-pulse electrical stimulation of the ipsilateral MoV. Subsequent whole cell recordings were generated from the screened SupV neurons, and their antidromic responses to MoV stimulation were confirmed. We divided the antidromically activated premotor neurons into two groups according to their discharge patterns during the steady state in response to 1-s depolarizing current pulses: those firing at a frequency higher (HF neurons, n = 19) or lower (LF neurons, n = 17) than 33 Hz. In addition, HF neurons had a narrower action potential and a larger afterhyperpolarization than LF neurons. Intracellular labeling revealed that the axons of all HF neurons (6/6) and half of the LF neurons (4/9) entered the MoV from its dorsomedial aspect, whereas the axons of the remaining LF neurons (5/9) entered the MoV from its dorsolateral aspect. Furthermore, the dendrites of three HF neurons penetrated into the principal sensory trigeminal nucleus (Vp), whereas the dendrites of all LF neurons were confined within the SupV. These results suggest that the types of SupV premotor neurons targeting the MoV with different firing properties have different dendritic and axonal morphologies, and these SupV neuron classes may play unique roles in diverse oral motor behaviors, such as suckling and mastication. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
453. Asymmetric synthesis of tetrahydroisoquinolines by enzymatic Pictet-Spengler reaction.
- Author
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Masakatsu Nishihachijo, Yoshinori Hirai, Shigeru Kawano, Akira Nishiyama, Hiromichi Minami, Takane Katayama, Yoshihiko Yasohara, Fumihiko Sato, and Hidehiko Kumagai
- Subjects
TETRAHYDROISOQUINOLINES ,PICTET-Spengler reaction ,ESCHERICHIA coli ,COPTIS japonica ,STEREOSELECTIVE reactions - Abstract
The article studies the synthesis of tetrahydroisoquinolines by enzymatic Pictet-Spengler reaction. The stydy uses an N-terminally truncated (S)-norcoclaurine synthase (NCS) from Coptis japonica expressed in Escherichia coli, where NCS catalyzes stereoselective Pictet-Spengler reaction for benzylisoquinoline alkaloid synthesis in plants. The study reflects versatility of the enzyme in synthesizing high yields of 6,7-dihydroxy-l-phenethyl-l,2,3,4-tetrahydroisoquinoline.
- Published
- 2014
- Full Text
- View/download PDF
454. Screening of Isoquinoline Alkaloids for Potent Lipid Metabolism Modulation with Caenorhabditis elegans.
- Author
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Yit-Lai CHOW and Fumihiko SATO
- Subjects
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ISOQUINOLINE , *LIPID metabolism disorders , *NEMATODES , *CAENORHABDITIS elegans , *BERBERINE , *SANGUINARINE - Abstract
The article offers information on screening of presence of isoquinoline alkaloids for determining the potent lipid metabolism modulation with Caenorhabditis elegans, a free-living, transparent nematode. Topics discussed include lipid metabolism modulators with berberine, lipid metabolism and treatment of elegans infection with berberine and sanguinarine.
- Published
- 2013
- Full Text
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455. Simple and Rapid Screening Method for Photosystem II Inhibitory Herbicides Using Photoautotrophically Cultured Plant Cells with Chlorophyll Fluorescence Monitoring
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Noboru Maejima, Katsunori Ichinose, Tadao Asami, Nobuya Tokutake, Koichi Yoneyama, Shigeo Yoshida, Fumihiko Sato, Hajime Iwamura, Makoto Konnai, Masaru Ogasawara, and Yoshihiro Nakajima
- Subjects
biology ,Photosystem II ,Organic Chemistry ,food and beverages ,macromolecular substances ,General Medicine ,biology.organism_classification ,Plant cell ,Photosynthesis ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Thylakoid ,Chlorophyll ,Botany ,polycyclic compounds ,Bioassay ,Chenopodiaceae ,Molecular Biology ,Chlorophyll fluorescence ,Biotechnology - Abstract
The photosystem II inhibitory activity of substituted phenylurea derivatives in liverwort photoautotrophically cultured cells was determined by monitoring chlorophyll fluorescence. Since the inhibitory activities in this assay correlated well with both the Hill inhibitory activity in isolated thylakoids and the herbicidal activity against mung bean plants, this bioassay may be a suitable screening method for photosystem II inhibitory herbicides.
- Published
- 1993
456. Corrigendum to: EM574, an erythromycin derivative, improves delayed gastric emptying of semi-solid meals in conscious dogs [Eur. J. Pharmacol. 395 (2000) 165–172.]
- Author
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Fumihiko Sato, Zen Itoh, Nobuhiro Inatomi, Satoshi Ōmura, and Shogo Marui
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Pharmacology ,chemistry.chemical_compound ,chemistry ,Gastric emptying ,medicine ,Erythromycin ,Derivative (chemistry) ,Semi solid ,medicine.drug - Published
- 2000
457. CYP719A subfamily of cytochrome P450 oxygenases and isoquinoline alkaloid biosynthesis in Eschscholzia californica.
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Kinuko Iwasa and Fumihiko Sato
- Subjects
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CYTOCHROME P-450 , *OXYGENASES , *ISOQUINOLINE , *ALKALOID synthesis , *CALIFORNIA poppy , *GENE expression - Abstract
Abstract Eschscholzia californica produces various types of isoquinoline alkaloids. The structural diversity of these chemicals is often due to cytochrome P450 (P450) activities. Members of the CYP719A subfamily, which are found only in isoquinoline alkaloid-producing plant species, catalyze methylenedioxy bridge-forming reactions. In this study, we isolated four kinds of CYP719A genes from E. californica to characterize their functions. These four cDNAs encoded amino acid sequences that were highly homologous to Coptis japonica CYP719A1 and E. californica CYP719A2 and CYP719A3, which suggested that these gene products may be involved in isoquinoline alkaloid biosynthesis in E. californica, especially in methylenedioxy bridge-forming reactions. Expression analysis of these genes showed that two genes (CYP719A9 and CYP719A11) were preferentially expressed in plant leaf, where pavine-type alkaloids accumulate, whereas the other two showed higher expression in root than in other tissues. They were suggested to have distinct physiological functions in isoquinoline alkaloid biosynthesis. Enzyme assay analysis using recombinant proteins expressed in yeast showed that CYP719A5 had cheilanthifoline synthase activity, which was expected based on the similarity of its primary structure to that of Argemone mexicana cheilanthifoline synthase (deposited at DDBJ/GenBank™/EMBL). In addition, enzyme assay analysis of recombinant CYP719A9 suggested that it has methylenedioxy bridge-forming activity toward (R,S)-reticuline. CYP719A9 might be involved in the biosynthesis of pavine- and/or simple benzylisoquinoline-type alkaloids, which have a methylenedioxy bridge in an isoquinoline ring, in E. californica leaf. [ABSTRACT FROM AUTHOR]
- Published
- 2009
458. Chloroplastic NAD(P)H Dehydrogenase Complex and Cyclic Electron Transport around Photosystem I.
- Author
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Tsuyoshi Endo, Satoshi Ishida, Noriko Ishikawa, and Fumihiko Sato
- Abstract
Recent molecular genetics studies have revealed that cyclic electron transport around photosystem I is essential for normal photosynthesis and growth of plants. Chloroplastic NAD(P)H dehydorgenase (NDH) complex, a homologue of the complex I in respiratory electron transport, is involved in one of two cyclic pathways. Recent studies on the function and structure of the NDH complex are reviewed. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
459. Role of endogenous basic fibroblast growth factor and angiogenesis in the healing of gastric ulcers in rats
- Author
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Fumihiko Sato, Akio Shina, Hiroshi Satoh, Rika Maeda, and Shoichi Asano
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Pharmacology ,chemistry.chemical_compound ,chemistry ,business.industry ,Angiogenesis ,Basic fibroblast growth factor ,Cancer research ,Medicine ,Endogeny ,business - Published
- 1995
460. Knockdown of berberine bridge enzyme by RNAi accumulates ( S )-reticuline and activates a silent pathway in cultured California poppy cells.
- Author
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Nanae Fujii, Takayuki Inui, Kinuko Iwasa, Takashi Morishige, and Fumihiko Sato
- Abstract
Abstract  Reticuline is a key compound in the biosynthetic pathway for isoquinoline alkaloids in plants, which include morphine, codeine and berberine. We established cultured California poppy (Eschscholzia californica) cells, in which berberine bridge enzyme (BBE) was knocked down by RNA interference, to accumulate the important key intermediate reticuline. Both BBE mRNA accumulation and enzyme activity were effectively suppressed in transgenic cells. In these transgenic cells, end-products of isoquinoline alkaloid biosynthesis, such as sanguinarine, were considerably reduced and reticuline was accumulated at a maximum level of 310 μg/g-fresh weight. In addition, 1 g-fresh weight of these cells secreted significant amounts of reticuline into the medium, with a maximum level of 6 mg/20 mL culture medium. These cells also produced a methylated derivative of reticuline, laudanine, which could scarcely be detected in control cells. We discuss the potential application of RNAi technology in metabolic modification and the flexibility of plant secondary metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
461. Overexpression of Coptis japonica Norcoclaurine 6-O-Methyltransferase Overcomes the Rate-Limiting Step in Benzylisoquinoline Alkaloid Biosynthesis in Cultured Eschscholzia californica.
- Author
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Fumihiko Sato
- Subjects
- *
PAPAVERACEAE , *BIOCHEMICAL engineering , *ALKALOIDS , *BIOCHEMISTRY - Abstract
Benzylisoquinoline alkaloids are one of the most important secondary metabolite groups, and include the economically important analgesic morphine and the antimicrobial agent berberine. To improve the production of these alkaloids, we investigated the effect of the overexpression of putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis. We introduced two O-methyltransferase [Coptis japonica norcoclaurine 6-O-methyltransferase (6OMT) and 3′-hydroxy-N-methylcoclaurine 4′-O-methyltransferase (4′OMT)] expression vectors into cultured California poppy cells to avoid the gene silencing effect of endogenous genes. We established 20 independent lines for 6OMT transformants and 15 independent lines for 4′OMT transformants. HPLC/liquid chromatography-mass spectrometry (LC-MS) analysis revealed that the overexpression of C. japonica 6OMT was associated with an average alkaloid content 7.5 times greater than that in the wild type, whereas the overexpression of C. japonica 4′OMT had only a marginal effect. Further characterization of 6OMT in California poppy cells indicated that a 6OMT-specific gene is missing and 4OMT catalyzes the 6OMT reaction with low activity in California poppy, which supports the notion that the 6OMT reaction is important for alkaloid biosynthesis in this plant species. We discuss the importance of 6OMT in benzylisoquinoline alkaloid biosynthesis and the potential for using a rate-limiting step gene to improve alkaloid production. [ABSTRACT FROM AUTHOR]
- Published
- 2007
462. Bowman–Birk Proteinase Inhibitor Confers Heavy Metal and Multiple Drug Tolerance in Yeast.
- Author
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Nobukazu Shitan, Ken-ichi Horiuchi, Fumihiko Sato, and Kazufumi Yazaki
- Subjects
YEAST ,CADMIUM ,GREEN fluorescent protein ,PROTEINASES - Abstract
Cultured Coptis japonica cells show tolerance to various toxic compounds. By yeast functional screening of cadmium (Cd) plates with its cDNA library, we isolated a gene encoding Bowman–Birk proteinase inhibitor (CjBBI). The yeast transformant of CjBBI showed multiple tolerance to various drugs adding to Cd, and revealed reduced Cd accumulation in cells. Preferential organs for Cjbbi expression were aerial parts of intact plants, and the subcellular localization of CjBBI was shown, using its green fluorescent protein fusion, to be the apoplast. Induction of Cjbbi expression by Cd treatment suggested that CjBBI was responsible for the tolerance to Cd observed in C. japonica cells. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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463. Identification of a WRKY Protein as a Transcriptional Regulator of Benzylisoquinoline Alkaloid Biosynthesis in Coptis japonica.
- Author
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Nobuhiko Kato, Emilyn Dubouzet, Yasuhisa Kokabu, Sayumi Yoshida, Yoshimasa Taniguchi, Joseph Gogo Dubouzet, Kazufumi Yazaki, and Fumihiko Sato
- Subjects
COPTIS japonica ,ISOQUINOLINE ,ALKALOIDS ,BIOSYNTHESIS - Abstract
Selected cultured Coptis japonica cells produce a large amount of the benzylisoquinoline alkaloid berberine. Previous studies have suggested that berberine productivity is controlled at the transcript level of biosynthetic genes. We have identified a regulator of transcription in berberine biosynthesis using functional genomics with a transient RNA interference (RNAi) and overexpression of the candidate gene. The 24 primary candidate clones were selected from 1,014 expressed sequence tags (ESTs) that were obtained from a C. japonica cell line producing high levels of berberine. Further characterization of the expression profiles of these ESTs suggested that five ESTs would be good candidates as regulators of berberine production. A newly developed transient RNAi system with C. japonica protoplasts indicated that double-stranded RNA of an EST clone significantly reduced the level of transcripts of 3′-hydroxy N-methylcoclaurine 4′-O-methyltransferase. Sequence analysis showed that this EST encoded a group-II WRKY, and we named it CjWRKY1. When the effects of double-stranded RNA of the CjWRKY1 gene were examined in detail, a marked reduction in the transcripts of all genes involved in berberine biosynthesis was detected, whereas little effect was found in the transcript levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and chorismate mutase (CM) that are associated with primary metabolism. Ectopic expression of CjWRKY1 cDNA in C. japonica protoplasts clearly increased the level of transcripts of all berberine biosynthetic genes examined compared with control treatment, whereas the levels of GAPDH and CM were not affected. The functional role of CjWRKY1 as a specific and comprehensive regulator of berberine biosynthesis is discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
464. In Vivo15N-Enrichment of Metabolites in Suspension Cultured Cells and Its Application to Metabolomics.
- Author
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Kazuo Harada, Eiichiro Fukusaki, Takeshi Bamba, Fumihiko Sato, and Akio Kobayashi
- Subjects
CELLS ,AMINO acids ,HEREDITY ,BIOCHEMICAL engineering - Abstract
The incorporation of stable isotopes in suspension cultured cells is very simple and useful as a preliminary experimental method in the experimental scene of plant metabolomics to elucidate the metabolic profiles of mutants and transformants. Stable isotope methods would afford a dynamic explanation of turnover speed that would concern the metabolic flux. Utilization of suspension cultured cells allows genes to be easily induced or suppressed, culture conditions to be controlled, and samples to be easily prepared. Stable isotope tracing allows an index of metabolic flux to be obtained. Here we present an experiment feeding 15N-labeled inorganic salts to Arabidopsis(cell line T87) and Coptiscultured cells. Results of a comparison of 15N labeling ratios of amino acids derived from T87 cells cultured under light with those cultured in the dark corresponded to transcriptional expressions revealed by microarray experiments published previously, demonstrating the validity of this procedure. Furthermore, 15N labeling ratios of Coptiscultured cells revealed arginine and lysine metabolism inhibition, which should result in inhibition of polyamine biosynthesis and cell division. This very simple experiment allowed us to uncover metabolic dynamic features of the plant cell. Therefore this method is very useful for forming working hypotheses and experimental design. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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465. Heterologous Expression of a Mammalian ABC Transporter in Plant and its Application to Phytoremediation.
- Author
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Kazufumi Yazaki, Nao Yamanaka, Tsugumi Masuno, Satoshi Konagai, Shuji Kaneko, Kazumitsu Ueda, and Fumihiko Sato
- Subjects
PHYTOREMEDIATION ,INORGANIC compounds ,ETOPOSIDE ,CELL membranes - Abstract
Mammalian ATP-binding cassette (ABC) transporters involved in the multidrug-resistance of cancer cells can efflux cytotoxic compounds that show a wide variety of chemical structures and biological activities. Human multidrug resistance-associated protein (hMRP1) is one of the most intensively studied ABC transporters and many substrates have been identified, including both organic and inorganic compounds. In an attempt at novel ‘transport engineering’ using hMRP1 as a molecular pump, we established transgenic tobacco plants that showed clear resistance to cadmium and daunorubicin, although they were not resistant to etoposide, another known substrate of hMRP1. When expressed in tobacco cells, hMRP1 protein was localized at vacuolar membrane, while members of the MRP family are localized at plasma membrane in mammalian cells to reduce the cellular accumulation of various drugs. Thus, the hMRP1-expressing tobacco cells were able to take up these substrates across the tonoplast and sequestrate them in the vacuolar matrix. These results suggest that it may be possible to use the transgenic tobacco in phytoremediation, where a single transformation with an ABC transporter with broad substrate specificity should be effective for extracting various environmental pollutants including both organic and inorganic compounds, and accumulate them in the plant body. This should be advantageous for the remediation of a complex polluted environment, which is commonly found in the real world. [ABSTRACT FROM AUTHOR]
- Published
- 2006
466. Ribosomal RNA processing and an RNase R family member in chloroplasts of Arabidopsis.
- Author
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Masahiro Kishine, Atsushi Takabayashi, Yuri Munekage, Toshiharu Shikanai, Tsuyoshi Endo, and Fumihiko Sato
- Subjects
CHLOROPLASTS ,ARABIDOPSIS ,FLUORESCENCE ,RADIOACTIVITY ,GENOTYPE-environment interaction - Abstract
Abstract An Arabidopsis mutant rnr1, which has a defect in the basic genetic system in chloroplasts, was isolated using the screening of the high chlorophyll fluorescence phenotype. Whereas chlorophyll fluorescence and immunoblot studies showed the mutant had reduced activities of photosystems I and II, molecular characterization of the mutant suggested that a T-DNA insertion impaired the expression of a gene encoding a RNase R family member with a targeting signal to chloroplasts. Since RNase R family members have a 3'5' exoribonuclease activity, we examined the RNA profile in chloroplasts. In rnr1 the intercistronic cleavage between 23S and 4.5S rRNA was impaired, and a significant reduction in rRNA in chloroplasts was found, suggesting that RNR1 functions in the maturation of chloroplast rRNA. The present results suggest that defects in the genetic system in chloroplasts cause high chlorophyll fluorescence, pale green leaf, and marked reduction in the growth rate, whereas the levels of some chloroplast RNA were higher in rnr1 than in the wild-type. [ABSTRACT FROM AUTHOR]
- Published
- 2004
467. CND41, a chloroplast nucleoid protein that regulates plastid development, causes reduced gibberellin content and dwarfism in tobacco.
- Author
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Takeshi Nakano, Noriko Nagata, Takuma Kimura, Masayo Sekimoto, Hiroshi Kawaide, Shinya Murakami, Yasuko Kaneko, Hisashi Matsushima, Yuji Kamiya, Fumihiko Sato, and Shigeo Yoshida
- Abstract
CND41, a DNA binding protein of chloroplast nucleoids, may function as a negative regulator of chloroplast gene expression. The reduction of CND41 in an antisense transformant accelerated plastid development in shoot apex cells and early young leaves, and caused a dwarf phenotype and altered leaf morphology. Plant height and leaf shape could be restored almost to those of the wild type by application of gibberellins (GAs), clearly indicating that a reduction in GA content was a prime cause of the dwarf phenotype in CND41 antisense transformants. The transformants had reduced endogenous levels of active gibberellin (GA
1 ), a biologically active GA, compared to those of wild-type plants. Possible relationships between chloroplast development affected by CND41 function and GA biosynthesis are discussed. [ABSTRACT FROM AUTHOR]- Published
- 2003
- Full Text
- View/download PDF
468. Mechanism of Kinin Release from Human Low-Molecular-Mass-Kininogen by the Synergistic Action of Human Plasma Kallikrein and Leukocyte Elastase.
- Author
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Fumihiko SATO and Shigeharu NAGASAWA
- Published
- 1988
469. Congenital Cervical Cysts and Fistulas, 22 Cases
- Author
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Hitoshi Saito, Fumihiko Sato, Shigeru Miyoshi, Osamu Mizukoshi, and Yuichi Suzuki
- Subjects
medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,Cervical cysts ,medicine ,business ,Surgery - Abstract
(1) 昭和48年1月1日から同52年10月31日までの約5年間に, 当教室外来を受診した患者の約0.066%, 22例に先天性頸部嚢腫を認め, そのうち先天性正中頸嚢腫18例 (0.054%), 先天性側頸嚢腫4例 (0.012%) であった.(2) 年令別分布を見ると, 0~20才に73%の症例が含まれたが, 50才台の症例も見られ, 年令のみで容易に診断できないことも推察された.(3) 治療面では, 正中頸嚢腫手術例10例につき3例の再発を見た (再発率30%). しかし, 舌骨切除を行なわなかった9例中6例け再発せずに治癒しており, 舌骨切除は必ずしも, どの症例にも必要なものではなく, 今回再発した3例のような症例に限り必要であり, その診断が大切である. 側頸嚢腫手術例1例には再発を見ていない.
- Published
- 1978
470. Chondroma of maxilla - A case report
- Author
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Sachio Yoshida, Kazuo Uede, Toshihito Tsubokawa, Takehisa Saito, Hitoshi Saito, and Fumihiko Sato
- Subjects
Nasal cavity ,Right nasal cavity ,business.industry ,Anatomy ,respiratory system ,medicine.disease ,Resection ,medicine.anatomical_structure ,Otorhinolaryngology ,Ethmoid sinus ,Maxilla ,otorhinolaryngologic diseases ,medicine ,business ,Lateral wall ,Chondroma ,Histological examination - Abstract
A five-year-old female visited our hospital because of nasal obstruction and epiphora. The right nasal cavity was occupied by a soft gray tumor. After total resection of the tumor piece by piece, it was considered that the tumor originated from the lateral wall of the right nasal cavity and spread to the right maxillary sinus, ethmoid sinus, and nasal cavity. Histological examination revealed a benign Chondroma. As Chondroma tend to exhibit sarcomatous changes, long-term follow-up is essential.
- Published
- 1987
471. [Untitled]
- Author
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Kunio Yanohara, Fumihiko Sato, Makoto Hyuga, Ikuo Hisa, Yuichi Suzuki, Takashi Matsui, and Osamu Mizukoshi
- Subjects
Otorhinolaryngology - Published
- 1984
472. [Untitled]
- Author
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Sachio Yoshida, Kazuo Uede, Makoto Hyuga, Hideo Nishimura, Masayoshi Tachibana, Hiroshi Takenaka, Kunio Yanohara, Akira Saito, Hitoshi Saito, Hideaki Kida, Fumihiko Sato, Yasuo Hisa, and Osamu Mizukoshi
- Subjects
medicine.medical_specialty ,business.industry ,medicine ,Cancer ,Radiology ,Cervical esophagus ,business ,medicine.disease - Published
- 1983
473. Mass Examination for Early Detection of Laryngeal Cancer
- Author
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Fumihiko Sato
- Subjects
Oncology ,medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,Internal medicine ,medicine ,Early detection ,Cancer ,medicine.disease ,business - Published
- 1987
474. A binding model for phenylurea herbicides based on analysis of a Thr264 mutation in the D-1 protein of tobacco
- Author
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Yasuyuki Yamada, Fumihiko Sato, and Yoshio Shigematsu
- Subjects
Stereochemistry ,Health, Toxicology and Mutagenesis ,Mutant ,General Medicine ,Hill reaction ,Serine ,Hydrophobic effect ,chemistry.chemical_compound ,Membrane ,Bromacil ,chemistry ,Thylakoid ,Binding site ,Agronomy and Crop Science - Abstract
A new mutant thylakoid membrane with threonine substituted for serine at the 264th position of D-1 protein (Thr264 mutant) showed strong resistance to triazines, phenylureas, metribuzin, and bromacil; whereas mutants with glycine substituted at the 264th position (Gly264 mutant) showed little resistance to phenylureas. The mechanism by which phenylureas bind to D-1 protein was investigated by quantitative structure-activity relation (QSAR) analysis of Thr264 mutant thylakoid membranes and the results were compared with those for other thylakoid membranes. Inhibition of the Hill reaction increased with the hydrophobicity of the phenylureas, particularly in both of the resistant (Thr264 and Gly264) membranes, these membranes being more resistant to N -methoxy- N -methylphenylureas than to N,N -dimethyl derivatives. Our results confirm that the binding site for phenylureas considerably overlaps the site for triazines. Based on our experimental data and a model established for triazine binding in a bacterial reaction center, we speculate that hydrophobic interactions of the phenyl ring with D-1 protein and a hydrogen bond of the carbonyl oxygen with the peptide-NH of the 265th position are important for the herbicidal activity of phenylureas. In contrast, an additional hydrogen bond with the oxygen of serine 264 takes part in the binding of triazines, metribuzin, and bromacil.
- Published
- 1989
475. High-quality AlGaAs/GaAs quantum well with a short period (InGaAs)(GaAs) strained superlattice buffer layer
- Author
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Mikihiko Shimura, Koichi Imanaka, Fumihiko Sato, and Hiroshi Imamoto
- Subjects
Materials science ,Period (periodic table) ,business.industry ,Superlattice ,Condensed Matter Physics ,Laser ,Buffer (optical fiber) ,law.invention ,Inorganic Chemistry ,Quality (physics) ,law ,Materials Chemistry ,Optoelectronics ,Quantum efficiency ,business ,Layer (electronics) ,Quantum well - Abstract
The optical quality of AlGaAs/GaAs single quantum wells prepared by MBE has been remarkably improved by the introduction of an (InGaAs)(GaAs) strained superlattice buffer (SSLB) layer and an AlGaAs/GaAs superlattice buffer (SLB) layer. The PL measurement shows more than 5 times higher peak intensity compared with that of the SLB alone. A GRIN-SCH single quantum well 770 nm laser with both SSLB and SLB was also examined. A high internal quantum efficiency of 95% with a small cavity loss of α = 3cm-1 and a high differential quantum efficiency of 50% even for a 3200 μm long cavity have been achieved.
- Published
- 1989
476. Lipid compositions of photomixotrophic green calluses and chlorophyll deficient leaves of tobacco
- Author
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Fumihiko Sato, Toshiake Matsuzaki, Akira Koiwai, Yasuyuki Yamada, and Teruyoshi Nagao
- Subjects
Chloroplast ,Phosphatidylglycerol ,chemistry.chemical_classification ,chemistry.chemical_compound ,chemistry ,Chlorophyll ,Callus ,Botany ,Fatty acid ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
Among photomixotrophic green calluses tested (N. rustica. N. tobacum L. cv. BY-4 and Samsun), the callus of Samsun had the highest contents of chlorophyll and chloroplast lipids, such as monogalactosyldiglyceride (MGDG), digalactosyldiglyceride (DGDG), sulfoquinovosyldigly-ceride (SQDG) and phosphatidylglycerol (PG). However, the chlorophyll and chloroplast lipids in the green callus of Samsun were still 1/6 and 1/3 of that in the parent leaves, respectively. The relative content of a-linolenate in MGDG, DGDG and SQDG of the green calluses were higher than that of the white calluses. The ratios of hexadecatrienoate in MGDG and hexadeceno-ate (Δ3-trans) in PG in the green calluses were trace or less compared with that of the parent leaves. The crude lipids and total fatty acid contents of the chlorophyll deficient leaves (N. taba-cum L. cv. Consolation 402 and Dominant Aurea Su/su) were almost the same as those of the normal leaves (cv. BY-4 and Samsun), although the chlorophyll contents of the chlorophyll...
- Published
- 1984
477. A Clinical Study on Clenching
- Author
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Hiroshi Kato, Fumihiko Sato, Jun Ishikawa, Keiji Asano, and Kazuhiko Hayashi
- Subjects
business.industry ,Dentistry ,Cheek ,Mandibular first molar ,body regions ,Masseter muscle ,Clinical study ,stomatognathic diseases ,medicine.anatomical_structure ,stomatognathic system ,Periodontal disease ,Occlusion ,medicine ,Premolar ,business ,Rest (music) - Abstract
Trauma from occlusion has been considered an important factor of periodontal disease. Tooth clenching which closes the jaws involuntary under heavy pressure is recognized to produce a great destructive force.We must correctly judge whether patients have the habit of clenching for treatment of periodontal disease.However, patients are usually unware of the habit and dental science has not yet developed accurate creteria for diagnosis of clenching as of the present moment. The purpose of this study was to relate the cheek pressure, cheek indentation, and EMG of masseter muscle for clarifying the subject of clenching.The experiments were carried out on 7 subjects from 28 to 45 years. When clinically examined, they seemed to have the habit of clenching, and tooth indentation was recognizable on cheek mucosa. Buccal pressure in the upper first molar and premolar region, and electrical activity of the masseter muscle were messured at: (1) rest, (2) saliva swallow, (3) water swallow, (4) voluntary clenching (closing the jaws voluntarily), and (5) emotional stress.The results and conclusion were as follows:(1) At rest; increase of cheek pressure was recognized during involuntary swallow, but occasionally between each involuntary swallows.(2), (3) During saliva and water swallow (voluntary swallow), cheek pressure was appreciably increased in all subjects.(4) During voluntary clenching, electrical activity of the masseter muscle was recognizable, but cheek pressure was not remarkably increased.(5) During emotional stress, cheek pressure increased more than rest in four subjects. These phenomena seems to be identifiable in the tension of the orofacial muscles by emotional stress.
- Published
- 1978
478. AlGaAs/GaAs superlattice multi-quantum-well laser diode
- Author
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Mikihiko Shimura, Hiroshi Imamoto, Koichi Imanaka, and Fumihiko Sato
- Subjects
Materials science ,Laser diode ,business.industry ,Superlattice ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,Condensed Matter Physics ,law.invention ,Active layer ,Condensed Matter::Materials Science ,Wavelength ,law ,Monolayer ,Optoelectronics ,General Materials Science ,Quantum well laser ,Electrical and Electronic Engineering ,business ,Molecular beam epitaxy ,Diode - Abstract
Short period (AlGaAs) m (GaAs) n (subscripts should stand for the monolayer number) superlattices have been applied to the active layer in a GRIN-SCH multi-quantum well laser diode grown by molecular beam epitaxy. A short wavelength of 720nm and cw operation at temperatures over 100°C were achieved, with a low threshold current of 24mA and high differential quantum efficiency of 75% at room temperature.
- Published
- 1989
479. An Improved Medium for the Photoautotrophic Culture ofCytisus scopariusLink Cells
- Author
-
Fumihiko Sato, Naruhiko Nakagawa, Tadashi Tanio, and Yasuyuki Yamada
- Subjects
General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Published
- 1981
480. [Untitled]
- Author
-
Makoto Hyuga, Kunio Yanohara, Fumihiko Sato, Yuichi Suzuki, Yasuo Hisa, and Satoru Takenouchi
- Subjects
Contraction time ,Glottis ,business.industry ,Anatomical structures ,Biomechanics ,Stimulation ,Anatomy ,respiratory system ,Intrinsic laryngeal muscle ,medicine.anatomical_structure ,Otorhinolaryngology ,Laryngeal Muscle ,Medicine ,Constant voltage ,business - Abstract
A study was made of the mechanical properties of the canine intrinsic laryngeal muscles; thyroarytenoid, cricothyroid, posterior cricoarytenoid, and lateral cricoarytenoid. These muscles were stimulated electrically with constant voltage stimulation of 1 Volt.The contraction time was found to differ between the relatively fast group (thyroarytenoid, 14 msec; lateral cricoarytenoid, 21 msec) and the relatively slow group (cricothyroid, 40 msec; posterior cricoarytenoid, 45 msec). The fusion frequency was also found to differ between the two groups, i. e. higher in the former (95Hz and 90Hz, respectively) and lower in the latter (40Hz and 45Hz, respectively). There was a certain correlation between the contraction time and the fusion frequency; the shorter the contraction time was, the higher was the fusion frequency.The mechanical properties of the laryngeal muscles were compared with the movement and biomechanics of the glottis induced by stimulation of the laryngeal nerve branch distributing to each intrinsic laryngeal muscle. The results indicated that the laryngeal movements and biomechanics, especially the glottic pressure, the size of the glottic chink and the adduction-abduction tension were affected significantly by the mechanical properties of the intrinsic laryngeal muscles and other anatomical structures such as the so-called elsetic recoil.
- Published
- 1982
481. Substance P Nerve Fibres in the Canine Larynx by PAP Immunohistochemistry
- Author
-
Yasuhiko Ibata, Osamu Mizukoshi, Fumihiko Sato, Kenji Fukui, and Yasuo Hisa
- Subjects
Male ,Larynx ,Pathology ,medicine.medical_specialty ,Connective tissue ,Substance P ,Immunoenzyme Techniques ,Superior laryngeal nerve ,chemistry.chemical_compound ,Dogs ,Nerve Fibers ,Recurrent laryngeal nerve ,Animals ,Medicine ,business.industry ,Laryngeal Nerves ,General Medicine ,Anatomy ,Epithelium ,medicine.anatomical_structure ,Otorhinolaryngology ,chemistry ,Laryngeal Mucosa ,Laryngeal Muscle ,Immunohistochemistry ,Female ,Laryngeal Muscles ,business - Abstract
The distribution of the Substance P (SP) immunoreactive nerve fibres in the canine larynx and laryngeal nerves was studied by PAP immunohistochemistry. Many individual SP immunoreactive nerve fibres with varicosities were observed within the epithelial layer and in the connective tissue below the epithelium of the laryngeal mucosa. Small numbers of SP immunoreactive nerve fibres were also found in the submucosal gland region and some of them appeared to terminate in glandular cells. These findings are consistent with the view that SP might be involved in the laryngeal sensory innervation system and the laryngeal glandular secretion. No SP immunoreactive nerve fibres were found in any intrinsic laryngeal muscles. The recurrent laryngeal nerve and the superior laryngeal nerve contained SP immunoreactive nerve fibres and were considered to lie in the pathway of the SP nerve fibres to the larynx.
- Published
- 1985
482. A Case of Congenital External Parotid Grand Fistula
- Author
-
Seiichi Komiya, Fumihiko Sato, and Norio Yasuda
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Fistula ,Physical examination ,medicine.disease ,Oral cavity ,Surgery ,stomatognathic system ,Otorhinolaryngology ,Rare case ,medicine ,Foramen ,Cyst ,Sialography ,Small hole ,business - Abstract
A rare case of congenital external parotid grand fistula was seen in a fourteen-year-old girl admitted with the complaint of salivation from a small hole in the left cheek. The salivation continued without stopping and increased especially after a meal. Stenon's foramen was situated at the normal position in her oral cavity and salivation was normal. She had no other congenital deformity.On physical examination, a cyst of 1.5cm in diameter was palpable at the back of the fistula. Sialography clearly revealed a cyst and fistula.A surgical procedure of extraction of the cyst which had an abnormal salivary duct was performed and the fistula on the left cheek was closed.The postoperative course was good and at present, 10 months after the surgery, sialography shows no fistula and she is free from any recurrence of the problem.
- Published
- 1980
483. [Untitled]
- Author
-
Takashi TAKAHAMA, Eiki JIDAI, and Fumihiko SATO
- Subjects
Polymers and Plastics ,Materials Science (miscellaneous) ,Chemical Engineering (miscellaneous) ,General Environmental Science - Published
- 1980
484. [Untitled]
- Author
-
Kunio Yanohara, Fumihiko Sato, Makoto Hyuga, Satoru Takenouchi, Yuichi Suzuki, and Yasuo Hisa
- Subjects
Glottis ,medicine.anatomical_structure ,Intrinsic laryngeal muscle ,Contraction (grammar) ,business.industry ,Biomechanics ,Medicine ,Anatomy ,business - Published
- 1982
485. Cholesterol Granuloma in the Posterior Ethmoid Sinus
- Author
-
Hitoshi Saito, Takehisa Saito, Hiroshi Takenaka, Akira Saito, Fumihiko Sato, and Kazuo Uede
- Subjects
Cholesterol granuloma ,Otorhinolaryngology ,business.industry ,Medicine ,Anatomy ,business ,Posterior ethmoid sinus - Published
- 1988
486. [Untitled]
- Author
-
Kazuo Uede, Seiichi Komiya, Fumiko Tanimura, Fumihiko Sato, and Hitoshi Saito
- Subjects
Otorhinolaryngology - Published
- 1982
487. [Untitled]
- Author
-
Osamu Mizukoshi, Nobuaki Yamashita, Fumihiko Sato, and Hitoshi Saito
- Subjects
Pathology ,medicine.medical_specialty ,Text mining ,Otorhinolaryngology ,business.industry ,medicine ,Unknown primary ,Cancer ,business ,Bioinformatics ,medicine.disease - Published
- 1983
488. An Electromyographic Study of Aspiration
- Author
-
Fumihiko Sato
- Subjects
Superior laryngeal nerve ,Swallowing ,business.industry ,Vagal nerve ,Laryngeal elevation ,Anesthesia ,Laryngeal Muscle ,Recurrent laryngeal nerve ,Fully conscious ,Medicine ,Anatomy ,business - Abstract
Aspiration may be caused by a variety of inco-ordination of swallowing.In this experimental design, a physiologic and biomechanical method for observation of the laryngopharyngeal motion was devised. That is, electromyographic observations of the laryngeal and pharyngeal constrictor muscles, measurement of glottic preseure and measurement of tensile force by laryngeal elevation were made in fully conscious and slightly anesthetized animals.These electromyographic and biomechanical observations of normal deglutition in the dog were characterized by a co-ordinated pattern of sequent three motions; laryngeal elevation, laryngeal closure and transportation of the bolus.Experimentally, aspiration was brought about by cutting superior laryngeal nerve, recurrent laryngeal nerve, vagal nerve and hypoglossal nerve.In the series of bilaterally sacrificed internal branch of the superior laryngeal nerves, the electrical phasic activity of the intrinsic laryngeal muscles and laryngeal closure were delayed about 90msec. during swallowing in slightly anesthetized condition, and in the series of bilaterally sectioned recurrent laryngeal nerves, degree of the laryngeal closure was markedly disturbed (34%). And also, degree of the laryngeal elevation at swallowing was 48% in the series of bilaterally sectioned hypoglossal nerves.From these experimental observations, mechanism of aspiration was described and discussed in clinical relation to aspiration.
- Published
- 1980
489. An improved medium for the photoautotrophic culture of Cytisus scoparius link cells
- Author
-
Naruhiko Nakagawa, Fumihiko Sato, Tadashi Tanio, and Yasuyuki Yamada
- Subjects
Cytisus scoparius ,chemistry.chemical_classification ,Basal medium ,biology ,fungi ,food and beverages ,Cytisus ,Phosphate ,biology.organism_classification ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,chemistry ,Auxin ,Botany ,Cytokinin ,General Agricultural and Biological Sciences - Abstract
The photoautotrophic culture medium for Cytisus scoparius Link cells was improved. Linsmaier and Skoog medium was modified by adding a 4-fold concentration of phosphate and lowering the auxin concentration (a-naphthaleneacetic acid 10−6M). Concentrations of all the inorganic elements except phosphate in the original basal medium were adequate for the photoautotrophic growth of Cytisus cells. A high concentration of cytokinin compensated for the inhibition of growth caused by a high concentration of auxin.
- Published
- 1981
490. Experimental Studies of the Laryngeal Transpantation
- Author
-
Fumihiko SATO
- Subjects
Otorhinolaryngology - Published
- 1974
491. Phagocytosis in Plant Protoplasts
- Author
-
Yasuyuki Yamada, Fumihiko Sato, Yoko Tan, and Katsumi Ueda
- Subjects
biology ,Physiology ,Nicotiana tabacum ,Phagocytosis ,fungi ,food and beverages ,Cell Biology ,General Medicine ,Vacuole ,biochemical phenomena, metabolism, and nutrition ,Protoplast ,equipment and supplies ,biology.organism_classification ,Cell biology ,Microbiology ,Cytoplasm ,bacteria ,Molecular Biology - Abstract
Protoplasts from mesophyll cells from leaves of Nicotiana tabacum and cultured cells of Rauwolfia serpentina were used. When polyethylene glycol was added to a mixture of these protoplasts, some protoplasts phagocytotically ingested another whole protoplast into their vacuoles. Phagocytosis occurred not only between protoplasts of the same species but also between those of different species. At the initial contact of two protoplasts, the cytoplasm between the plasma membrane and the tonoplast of the ingesting protoplast migrated from the area of contact and an invagination developed containing the other protoplast which was finally incorporated into the vacuole by closure of the invagination.
- Published
- 1978
492. Summary Reconstruction by Cervical Island Skin Flap Following Resection of Floor of Mouth
- Author
-
Hideo Nishimura, Hitoshi Saito, Yuko Kawamura, Noboru Asano, Noriko Kawai, Fumihiko Sato, Akira Saito, and Osamu Mizukoshi
- Subjects
medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,Medicine ,business ,Dermatology - Published
- 1974
493. Reconstructive surgery for recurrent laryngeal nerve paralysis
- Author
-
Taisuke Kurokawa, Toshihito Tsubokawa, Sachio Yoshida, Fumihiko Sato, Hiroshi Takenaka, Osamu Mizukoshi, Takehisa Saito, Nobuo Matsumoto, Hitoshi Saito, Kazuo Uede, Nizo Takanami, and Yasuo Hisa
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Thyroidectomy ,Neurapraxia ,Arytenoid cartilage ,respiratory system ,Vocal process ,medicine.disease ,Surgery ,Neurotmesis ,medicine.anatomical_structure ,Anesthesia ,otorhinolaryngologic diseases ,Paralysis ,medicine ,Recurrent laryngeal nerve ,Axonotmesis ,medicine.symptom ,business - Abstract
As functional reconstructive surgeries for recurrent laryngeal nerve paralysis, there are static reconstruction such as intracordal silicone injection or Woodmann's operation, and dynamic reconstruction such as free nerve grafting and pedicle nerve muscle grafting.This paper is mainly concerned with the present situation and indication of each surgical procedure for recurrent laryngeal nerve paralysis. In order to clear the indication of the surgery, recurrent laryngeal nerve paralysis was classified pathophysiologically into four degrees: I. Condition of neurapraxia or its combination with axonotmesis, in which vocal cord movement might be recovered. II. Condition of axonotmesis or its combination with few neurotmesis, in which atrophy of the vocal cord exists and the movement could not be recovered. III. Condition of neurotmesis, in which atrophy and high level of the vocal cord exist. IV. Condition of severe neurotmesis, in which the vocal cord atrophies progressively.The mediofixation of the vocal cord for unilateral paralysis is indicated to vocal cord atrophy (II grade) and mediofixation of the vocal process by adduction of the arytenoid cartilage is indicated to the high level vocal cord. Dynamic reconstruction is needed for unilateral nerve defect at thyroidectomy. Surgical procedure for bilateral paralysis is selected depending on the degree of dyspnea.
- Published
- 1986
494. [Untitled]
- Author
-
MASAYOSHI TACHIBANA, HITOSHI SAITO, FUMIHIKO SATO, HIDEKI KIDA, and OSAMU MIZUKOSHI
- Subjects
Otorhinolaryngology - Published
- 1981
495. Electric strength of epoxide resins and its relation to the structure
- Author
-
Fumihiko Sato, Osamu Hayashi, and Takashi Takahama
- Subjects
chemistry.chemical_classification ,Electric strength ,Range (particle radiation) ,Materials science ,Polymers and Plastics ,Epoxide ,Ether ,General Chemistry ,Polymer ,Surfaces, Coatings and Films ,Ion ,chemistry.chemical_compound ,Volume (thermodynamics) ,chemistry ,Polymer chemistry ,Materials Chemistry ,Composite material ,Glass transition - Abstract
Epoxide resins having various ratios of ether and ester bonds were investigated as to the relation between electric strength and polymer characteristics. The electric strength over a wide range of temperature is presented here. A marked reduction of strength characteristics of the epoxide resins occurs at a critical temperature indistinguishable from the glass transition temperature Tg, which is related to the free volume and molecular relaxation process. At temperatures exceeding Tg, the electric strength has a strong dependence on polymer structure, film thickness, and applied pulse width. This behavior is considered to obey the thermal breakdown mechanism, and it is assumed that the ion is important in the precursory region of electric breakdown.
- Published
- 1981
496. A comparison of effects of several herbicides on photoautotrophic, photomixotrophic and heterotrophic cultured tobacco cells and seedlings
- Author
-
Satomi Takeda, Yasuyuki Yamada, and Fumihiko Sato
- Subjects
Nicotiana tabacum ,Bialaphos ,Plant Science ,General Medicine ,Biology ,biology.organism_classification ,Photosynthesis ,chemistry.chemical_compound ,chemistry ,Paraquat ,Glyphosate ,Dinoseb ,Botany ,Atrazine ,Agronomy and Crop Science ,Sodium chlorate - Abstract
The effects of herbicides with different primary modes of action were examined on the growth of photoautotrophic, photomixotrophic, and heterotrophic cultures of tobacco cells. These responses were compared with those of tobacco seedlings to the same herbicides. Herbicides, which primarily inhibit or disturb photosynthetic processes, suppressed the growth of photoautotrophic cells most strongly, as compared to photomixotrophic and heterotrophic cells (atrazine, diuron, paraquat). Herbicides having a primary mode of action other than the inhibition of photosynthetic processes, suppressed the growth of all types of cultured cells at similar concentrations (2,4-D, diphenamid, glyphosate, dinoseb, sodium chlorate, bialaphos, DTP), but the photoautotrophic cells were still the most sensitive to all kinds of herbicides except sodium chlorate. Furthermore, photoautotrophic cells responded to most of the herbicides as did the seedlings, with the exception of glyphosate and diphenamid. The possibility of photoautotrophically cultured cells as a model system to study the effects of herbicides are discussed.
- Published
- 1987
497. Functional Reconstruction for Unilateral Recurrent Laryngeal Nerve Paralysis Caused by Thyroid Cancer
- Author
-
Hitoshi Saito and Fumihiko Sato
- Subjects
Adult ,medicine.medical_specialty ,Reconstructive surgery ,medicine.medical_treatment ,Dogs ,Phonation ,Paralysis ,medicine ,Recurrent laryngeal nerve ,Animals ,Humans ,Thyrohyoid muscle ,Thyroid Neoplasms ,Surgery, Plastic ,Thyroid cancer ,Aged ,Recurrent Laryngeal Nerve ,business.industry ,Thyroidectomy ,Laryngeal Nerves ,General Medicine ,Middle Aged ,medicine.disease ,Muscle atrophy ,Surgery ,medicine.anatomical_structure ,Otorhinolaryngology ,Anesthesia ,Female ,medicine.symptom ,business ,Vocal Cord Paralysis ,Reinnervation - Abstract
Surgery for unilateral recurrent laryngeal nerve paralysis, which is caused by thyroid cancer, appearing within the past six months, should be aimed at improvement of the asymmetrical vocal cord and preservation of the stiffness and the mass of the vocal fold. To accomplish these aims, reconstructive surgery of the neuromuscular systems is available and it should be performed at the same time as thyroidectomy. Our surgical procedures for unilateral recurrent laryngeal nerve paralysis caused by thyroid cancer are reported along with some experimental investigations in dogs. Experimentally, muscle atrophy was prevented by neurorrhaphy or by free nerve grafting of the recurrent laryngeal nerve, although movement of the vocals cord was not recovered due to misdirected reinnervation and reduction of the end plates. In our study of functional reconstruction for unilateral recurrent laryngeal nerve paralysis, end-to-end anastomosis or free nerve grafting between the recurrent laryngeal nerve after extirpating the abductor muscle branch of this nerve was the most effective and practical method for improvement of the adductor function. Clinically reconstructive surgery was performed on five females suffering from thyroid cancer with unilateral recurrent laryngeal nerve paralysis. The abductor branch was cut selectively in four cases, followed by free nerve grafting of the ansa cervicalis and pedicle nerve muscle graft of the thyrohyoid muscle implanted in one case. Vocal cord atrophies were not observed and phonations were good in all cases six months after of operation. However, obvious adduction of the operated vocal fold at phonation was obtained only in two cases and other two cases showed slight adduction.
- Published
- 1985
498. A Case of Secondary Posterior Laryngeal Web
- Author
-
Kunio Yanohara, Makoto Hiuga, Fumihiko Sato, Akira Saito, Yuichi Suzuki, and Yasuo Mizuta
- Subjects
medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,General surgery ,Laryngeal web ,Medicine ,business - Published
- 1980
499. [Untitled]
- Author
-
Fumihiko Sato
- Subjects
Contraction (grammar) ,business.industry ,Medicine ,Anatomy ,business - Published
- 1981
500. Factors in the Development of Esophageal Speech
- Author
-
Osamu Mizukoshi, Fumihiko Sato, Fumio Nakamura, Takeshige Nishimura, Keiichi Date, and Hitoshi Saito
- Subjects
Larynx ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Esophageal speech ,Audiology ,Electrolarynx ,Cricopharyngeal muscle ,Laryngectomy ,medicine.anatomical_structure ,Otorhinolaryngology ,Laryngoplasty ,Voice Training ,otorhinolaryngologic diseases ,medicine ,Phonation ,business - Abstract
Patients who undergo laryngectomy lose the facility of phonation. In these patients, compensatory voice may be produced by the use of body structures (ex. esophageal speech or laryngoplasty) or with the help of special aids such as an artificial larynx or an electro-larynx. The majority of patients who go through with voice training after the operation learn to speak with an esophageal type voice. However, it is difficult for these laryngectomized patients to master the esophageal speech which requires extensive training. Those patients who failed to learn this type of substitutive voice may be helped by the use of artificial instruments.We did a statistical study of the compensatory voices and of a few factors in the development of the esophageal speech in the members of KYOTO KOYUKAI (the Society of laryngectomized).The items of the compensatory voice utilized by these alaryngeal members were demonstrated. The esophageal speech was 51% (90 patients) out of 177 patients. A study of factors in the development of the esophageal speech revealed that in those over 70 years of age and in those with a personality such as introversion and neuroticism, it was difficult to master the esophageal speech. However, the surgical procedure of total laryngectomy and whether or not the suture of cricopharyngeal muscle should be done is not directly concerned with the development of esophageal speech.Table 1. COMPENSATORY VOICE IN MEMBERS OF KYOTO KOYUKAIEsophageal speech 79 cases (44.7%)Esophageal speech with artificial larynx 11 (6.3%) 90 cases (51%)Artificial larynx 62 (35.1%)Electrical larynx 2 (1.1%)Writing or sign 18 (9.9%)Unknown 2 (1.1%)Laryngoplasty 3 (1.8%)
- Published
- 1978
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